| obligate bacterial endosymbionts of acanthamoeba spp. related to the beta-proteobacteria: proposal of 'candidatus procabacter acanthamoebae' gen. nov., sp. nov. | all obligate bacterial endosymbionts of free-living amoebae currently described are affiliated with the alpha-proteobacteria, the chlamydiales or the phylum cytophaga-flavobacterium-bacteroides. here, six rod-shaped gram-negative obligate bacterial endosymbionts of clinical and environmental isolates of acanthamoeba spp. from the usa and malaysia are reported. comparative 16s rdna sequence analysis demonstrated that these endosymbionts form a novel, monophyletic lineage within the beta-proteobac ... | 2002 | 11931173 |
| a glycogen-debranching enzyme from cytophaga. | | 1970 | 11945550 |
| mutations in flavobacterium johnsoniae gldf and gldg disrupt gliding motility and interfere with membrane localization of glda. | flavobacterium johnsoniae moves rapidly over surfaces by a process known as gliding motility. the mechanism of this form of motility is not known. four genes that are required for f. johnsoniae gliding motility, glda, gldb, gldd, and ftsx, have recently been described. glda is similar to the atp-hydrolyzing components of atp binding cassette (abc) transporters. tn4351 mutagenesis was used to identify two additional genes, gldf and gldg, that are required for cell movement. gldf and gldg appear t ... | 2002 | 11948149 |
| partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces. | distinct partitioning has been observed in the composition and diversity of bacterial communities inhabiting the surface and overlying seawater of three coral species infected with black band disease (bbd) on the southern caribbean island of curaçao, netherlands antilles. pcr amplification and sequencing of bacterial 16s rrna genes (rdna) with universally conserved primers have identified over 524 unique bacterial sequences affiliated with 12 bacterial divisions. the molecular sequences exhibite ... | 2002 | 11976091 |
| response of endophytic bacterial communities in potato plants to infection with erwinia carotovora subsp. atroseptica. | the term endophyte refers to interior colonization of plants by microorganisms that do not have pathogenic effects on their hosts, and various endophytes have been found to play important roles in plant vitality. in this study, cultivation-independent terminal restriction fragment length polymorphism analysis of 16s ribosomal dna directly amplified from plant tissue dna was used in combination with molecular characterization of isolates to examine the influence of plant stress, achieved by infec ... | 2002 | 11976096 |
| spatial distribution of total, ammonia-oxidizing, and denitrifying bacteria in biological wastewater treatment reactors for bioregenerative life support. | bioregenerative life support systems may be necessary for long-term space missions due to the high cost of lifting supplies and equipment into orbit. in this study, we investigated two biological wastewater treatment reactors designed to recover potable water for a spacefaring crew being tested at johnson space center. the experiment (lunar-mars life support test project-phase iii) consisted of four crew members confined in a test chamber for 91 days. in order to recycle all water during the exp ... | 2002 | 11976099 |
| enrichment versus biofilm culture: a functional and phylogenetic comparison of polycyclic aromatic hydrocarbon-degrading microbial communities. | the effect that culture methods have on the diversity of degradative microbial communities is not well understood. we compared conventional batch enrichment with a biofilm culture method for the isolation of polycyclic aromatic hydrocarbon (pah)-degrading microbial communities from a pah-contaminated soil. the two methods were assessed by comparing: (i) the diversity of culturable bacteria; (ii) the diversity of pah-catabolic genes in isolated bacteria; (iii) the inter- and intraspecific diversi ... | 2002 | 12000317 |
| wide bacterial diversity associated with tubes of the vent worm riftia pachyptila. | we carried out a 16s rdna-based molecular survey of the prokaryotic diversity associated with the chitin tubes of the giant vent tubeworm riftia pachyptila (collected at the east pacific rise, 9 degrees n and 13 degrees n). scanning electron microscopy showed dense microbial populations, particularly on the external surface of the middle and upper tube regions, which included very diverse prokaryotic morphotypes. we used archaeal- and bacterial-specific primers for polymerase chain reaction (pcr ... | 2002 | 12010127 |
| community dynamics of free-living and particle-associated bacterial assemblages during a freshwater phytoplankton bloom. | bacterial community dynamics were followed in a 19-day period during an induced diatom bloom in two freshwater mesocosms. the main goal was to compare diversity and succession among free-living (<10 mm) and particle-associated (>10 mm) bacteria. denaturing gradient gel electrophoresis (dgge) of pcr amplified 16s rdna showed the highest number of bands among free-living bacteria, but with a significant phylogenetic overlap in the two size fractions indicating that free-living bacteria were also i ... | 2001 | 12024253 |
| changes in the epilimnetic bacterial community composition, production, and protist-induced mortality along the longitudinal axis of a highly eutrophic reservoir. | we studied changes in the epilimnetic bacterial community composition (bcc), bacterial biomass and production, and protistan succession and bacterivory along the longitudinal axis of the canyon-shaped, highly eutrophic sau reservoir (ne spain) during two sampling campaigns, in april and july 1997. longitudinal changes in bcc from the river inflow to the dam area of the reservoir were detected by using oligonucleotide probes targeted to the kingdom bacteria, to the alpha, beta, and gamma subclass ... | 2001 | 12024261 |
| phylogenetic analysis of microbial diversity in the rhizoplane of oilseed rape (brassica napus cv. westar) employing cultivation-dependent and cultivation-independent approaches. | the structure of the microbial rhizoplane community of the important crop plant oilseed rape was studied by using a culture-dependent as well as a culture-independent approach based on 16s rdna amplification. after isolation of the microbial community from the rhizoplane of oilseed rape (brassica napus cv. westar), the collected suspension was divided into two parts. one part was used for cultivation of bacteria onto three different growth media to establish a culture collection. from the other ... | 2001 | 12024277 |
| microbial community of a saline mud volcano at san biagio-belpasso, mt. etna (italy). | in san biagio of belpasso, approximately 20 km south of mt. etna, in the area of contact between volcanic and sedimentary formations, a number of small (3- 60 cm in diameter) active mud eruptions discharge co2-rich gases, mud and nacl brines. they can be described as mini-volcanoes owing to their typical conic shapes and continuously bubbling peak craters. samples were collected from the active peak craters at a depth of 20 cm and dna was immediately extracted and amplified with universal 16s rr ... | 2002 | 12030850 |
| analysis of endophytic bacterial communities of potato by plating and denaturing gradient gel electrophoresis (dgge) of 16s rdna based pcr fragments. | the diversity of endophytic bacterial populations of potato (solanum tuberosum cv desirée) was assessed using a combination of dilution plating of plant macerates followed by isolation and characterization of isolates, and direct pcr-dgge on the basis of dna extracted from plants. the culturable endophytic bacterial communities detected in potato stem bases as well as in roots were in most cases on the order 103 to 105 cfu g?1 of fresh plant tissue. dilution plating revealed that a range of bact ... | 2001 | 12032611 |
| contribution of cytophaga-like bacteria to the potential of turnover of carbon, nitrogen, and phosphorus by bacteria in the rhizosphere of barley (hordeum vulgare l.). | the functional potential of bacteria isolated from the rhizosphere of barley (hordeum vulgare l.) in may, july, and august and cultivated on nutrient-rich substrate (1/10 tsba) and nutrient-poor substrate (cold soil extract agar) was determined. there was no significant difference in numbers of cfu when counted on nutrient rich or poor substrate. bacterial numbers increased approximately 3-fold in the rhizosphere soil from may to august but was unchanged in bulk soil over the same period. a tota ... | 2002 | 12037608 |
| phylogenetic diversity of numerically important arctic sea-ice bacteria cultured at subzero temperature. | heterotrophic bacteria in sea ice play a key role in carbon cycling, but little is known about the predominant players at the phylogenetic level. in a study of both algal bands and clear ice habitats within summertime arctic pack ice from the chukchi sea, we determined the abundance of total bacteria and actively respiring cells in melted ice samples using epifluorescence microscopy and the stains 4', 6'-diamidino-2-phenylindole 2hcl (dapi) and 5-cyano-2,3-ditolyl tetrazolium chloride (ctc), res ... | 2002 | 12037610 |
| genetic diversity of attached bacteria in the hindgut of the deposit-feeding shrimp neotrypaea (formerly callianassa) californiensis (decapoda: thalassinidae). | microbial colonization of marine invertebrate guts is widespread, but in general the roles that these bacteria play in the nutrition of their hosts are unknown. to examine the diversity and potential nutritional roles of hindgut microbiota in a deposit feeder, pcr-amplified 16s rrna genes were cloned from the bacterial community attached to the hindguts of the thalassinid shrimp neotrypaea californiensis exposed to different feeding treatments. partial 16s rdna sequences were analyzed for 30 clo ... | 2002 | 12043003 |
| the branching order and phylogenetic placement of species from completed bacterial genomes, based on conserved indels found in various proteins. | the presence of shared conserved inserts and deletions (indels or signature sequences) in proteins provides a powerful means for understanding the evolutionary relationships among the bacteria. using such indels, all of the main groups within the bacteria can be defined in clear molecular terms and it has become possible to deduce that they branched from a common ancestor in the following order: low g + c gram-positive --> high g+c gram-positive --> deinococcus thermus --> cyanobacteria --> spir ... | 2001 | 12051562 |
| altamira cave paleolithic paintings harbor partly unknown bacterial communities. | since it has been reported that microorganisms can affect painting pigments, paleolithic painting microbiology deserves attention. the present study is the first report on the bacterial colonization of the valuable paleolithic paintings in the famous altamira cave (spain). one sample taken from a painting area in the polychromes hall was analyzed culture-independently. this was the first time microbiologists were allowed to take sample material directly from altamira paintings. identification me ... | 2002 | 12052543 |
| proposed minimal standards for describing new taxa of the family flavobacteriaceae and emended description of the family. | in this paper minimal standards for the description of new genera and cultivable species in the family flavobacteriaceae are proposed in accordance with recommendation 30b of the bacteriological code (1990 revision). in addition to specified phenotypic characteristics, the description of new species should be based on dna-dna hybridization data, and the placement of new taxa should be consistent with phylogenetic data derived from 16s rrna sequencing. an emended description of the family is also ... | 2002 | 12054224 |
| prokaryotic genetic diversity throughout the salinity gradient of a coastal solar saltern. | bacterial and archaeal assemblages have been studied in a multipond solar saltern using a range of microbial ecology techniques by four laboratories simultaneously. these include 16s rdna sequencing from both denaturing gradient gel electrophoresis (dgge) and clone libraries, and culturing methods. water samples from eight ponds were analysed, covering a salinity range from near sea water (4% salt) to saturated sodium chloride (37% salt; ponds called crystallizers). clone libraries focused on po ... | 2002 | 12071980 |
| cloning of a gene encoding raw-starch-digesting amylase from a cytophaga sp. and its expression in escherichia coli. | a raw-starch-digesting amylase (rsda) gene from a cytophaga sp. was cloned and sequenced. the predicted protein product contained 519 amino acids and had high amino acid identity to alpha-amylases from three bacillus species. only one of the bacillus alpha-amylases has raw-starch-digesting capability, however. the rsda, expressed in escherichia coli, had properties similar to those of the enzyme purified from the cytophaga sp. | 2002 | 12089056 |
| phylogenetic characterization of microbial communities that reductively dechlorinate tce based upon a combination of molecular techniques. | an anaerobic microbial consortium (referred to as anas) that reductively dechlorinates trichloroethene (tce) completely to ethene with the transient production of cisdichloroethene (cdce) and vinyl chloride was enriched from contaminated soil obtained from alameda naval air station. anas uses lactate as its electron donor and has been functionally stable for over 2 years. following a brief exposure to oxygen, a subculture (designated vcc) derived from anas could dechlorinate tce only to vinyl ch ... | 2002 | 12099461 |
| investigation of an anaerobic microbial community associated with a corneal ulcer by denaturing gradient gel electrophoresis and 16s rdna sequence analysis. | the bacterial community manifested in a corneal ulcer was investigated with culture-independent techniques. dna was extracted from the eye swab, 200-bp fragments spanning the hypervariable v3 region of the 16s rrna gene (16s rdna) were amplified by broad-range pcr and genetic fingerprinting of the total bacterial community was performed by denaturing gradient gel electrophoresis (dgge). additionally, 16s rdna clone libraries containing 1500-bp fragments were constructed, clones were screened by ... | 2002 | 12106952 |
| phylogenetic 16s rrna analysis reveals the presence of complex and partly unknown bacterial communities in tito bustillo cave, spain, and on its palaeolithic paintings. | tito bustillo cave (ribadesella, spain) contains valuable palaeolithic paintings, which date back 15 000-20 000 years. since 1969, the cave has been open to the public. rock wall surfaces, spelaeothems and soils are covered by apparent biofilms of phototrophic microorganisms, which develop under artificial lighting. in addition, rock surfaces present conspicuous bacterial growth in the form of round colonies of different colours and about 1-2 mm in diameter. even the famous paintings panel shows ... | 2002 | 12123475 |
| characterization of the bacterial consortium associated with black band disease in coral using molecular microbiological techniques. | the bacterial community associated with black band disease (bbd) of the scleractinian corals diploria strigosa, montastrea annularis and colpophyllia natans was examined using culture-independent techniques. two complementary molecular screening techniques of 16s rdna genes [amplified 16s ribosomal dna restriction analysis (ardra) of clone libraries and denaturing gradient gel electrophoresis (dgge)] were used to give a comprehensive characterization of the community. findings support previous s ... | 2002 | 12123476 |
| cysteine activation is an inherent in vitro property of prolyl-trna synthetases. | aminoacyl-trna synthetases are well known for their remarkable precision in substrate selection during aminoacyl-trna formation. some synthetases enhance the accuracy of this process by editing mechanisms that lead to hydrolysis of incorrectly activated and/or charged amino acids. prolyl-trna synthetases (prorss) can be divided into two structurally divergent groups, archaeal-type and bacterial-type enzymes. a striking difference between these groups is the presence of an insertion domain (appro ... | 2002 | 12130657 |
| primrose: a computer program for generating and estimating the phylogenetic range of 16s rrna oligonucleotide probes and primers in conjunction with the rdp-ii database. | we describe primrose, a computer program for identifying 16s rrna probes and pcr primers for use as phylogenetic and ecological tools in the identification and enumeration of bacteria. primrose is designed to use data from the ribosomal database project (rdp) to find potentially useful oligonucleotides with up to two degenerate positions. the taxonomic range of these, and other existing oligonucleotides, can then be explored, allowing for the rapid identification of suitable oligonucleotides. pr ... | 2002 | 12140334 |
| gelidibacter mesophilus sp. nov., a novel marine bacterium in the family flavobacteriaceae. | two gram-negative, aerobic, heterotrophic, marine bacteria, isolated from mediterranean sea water off the coast near valencia (spain), were the object of this study. these non-motile, yellow-pigmented, rod-shaped strains have been studied by means of dna-dna hybridization, 16s rrna sequencing and cultural and physiological features. phylogenetic analysis showed that both strains belong to the phylum cytophaga-flavobacterium-bacteroides, and their closest neighbour is the psychrophilic bacterium ... | 2002 | 12148647 |
| critical issues in bacterial phylogeny. | to understand bacterial phylogeny, it is essential that the following two critical issues be resolved: (i) development of well-defined (molecular) criteria for identifying the main groups within bacteria, and (ii) to understand how the different main groups are related to each other and how they branched off from a common ancestor. these issues are not resolved at present. we have recently described a new approach, based on shared conserved inserts and deletions (indels or signature sequences) f ... | 2002 | 12167362 |
| inhibition of lotic biofilms by diclofenac. | diclofenac, a common drug, was subjected to degradation studies using river biofilms grown in rotating annular reactors. degradation of diclofenac was possible after acclimatisation as confirmed by liquid chromatography-mass spectrometry analyses. adapted biofilms showed that degradation down to 10-25% of the initial concentration could be achieved within 4 days. in situ observation by confocal laser scanning microscopy, however, revealed slow biofilm development in the presence of diclofenac co ... | 2002 | 12172615 |
| interactions between the unicellular red alga rhodella reticulata (rhodophyta) and contaminated bacteria. | to define the role of the bacterial strains lr1 and lr3 in the rhodella cell destruction caused by cytophaga sp.lr2. | 2002 | 12174050 |
| phylogenetic diversity of winter bacterioplankton of eutrophic siberian reservoirs as revealed by 16s rrna gene sequence. | using 16s rrna gene sequence analyses we investigated the bacterial diversity of winter bacterioplankton of two eutrophic siberian reservoirs. these reservoirs show similarity in phytoplankton community composition in spring and autumn but tend to differ in summer in exhibiting cyanobacterial bloom. forty-eight unique partial 16s rna gene sequences retrieved from two libraries were mostly affiliated with the class actinobacteria, b subdivision of the class proteobacteria, and the phylum cytophag ... | 2002 | 12209253 |
| evaluation of methods to solubilize and analyze cell-associated ectoenzymes. | to quantify the functional diversity of microbes that use hydrolytic ectoenzymes, the feasibility of separating cell-associated proteins on polyacrylamide gels and detecting enzyme activity via fluorescent substrate analogs for aminopeptidases, glucosidases, and esterases was determined. more than 87% of aminopeptidase activity was associated with particulate cell material in all of the 10 gram-negative bacterial strains that were investigated. although 7-amino-4-methylcoumarin-leucine (amc-leuc ... | 2002 | 12223287 |
| cultivation-dependent characterization of bacterial diversity from british columbia forest soils subjected to disturbance. | bacteria from forest surface organic matter and mineral soil horizons were cultivated using four methods and characterized by fatty acid methyl ester (fame) analysis. soil samples from a british columbia ministry of forests long-term soil productivity (ltsp) installation were collected during winter and summer from two disturbance treatments (whole-tree harvesting with no soil compaction (plot n) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (p ... | 2002 | 12224563 |
| molecular characterization of bacterial diversity from british columbia forest soils subjected to disturbance. | bacteria from forest soils were characterized by dna sequence analysis of cloned 16s rrna gene fragments (16s clones). surface organic matter and mineral soil samples from a british columbia ministry of forests long-term soil productivity (ltsp) installation were collected during winter and summer from two disturbance treatments: whole-tree harvesting with no soil compaction (plot n) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (plot s). phylo ... | 2002 | 12224564 |
| limited selection of sodium channel blocking toxin-producing bacteria from paralytic shellfish toxin-contaminated mussels (aulacomya ater). | paralytic shellfish toxins (psts) are sodium channel blocking (scb) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. in a previous study, approximately 70% of the bacteria enriched from pst-contaminated shellfish tissue and isolated on marine agar medium were observed to produce scb toxins. in the study reported here, the high percentage of cultivable toxigenic bacteria is demonstrated ... | 2002 | 12238500 |
| succession of bacterial community structure along the changjiang river determined by denaturing gradient gel electrophoresis and clone library analysis. | bacterial community structure along the changjiang river (which is more than 2,500 km long) was studied by using denaturing gradient gel electrophoresis (dgge) and clone library analysis of pcr-amplified 16s ribosomal dna (rdna) with universal bacterial primer sets. dgge profiles and principal-component analysis (pca) demonstrated that the bacterial community gradually changed from upstream to downstream in both 1998 and 1999. bacterial diversity, as determined by the shannon index (h'), gradual ... | 2002 | 12324365 |
| aequorivita gen. nov., a member of the family flavobacteriaceae isolated from terrestrial and marine antarctic habitats. | several strains isolated from antarctic winter sea water, sea-ice algal assemblages and quartz stone subliths were found to belong to a novel 16s rdna sequence cluster within the family flavobacteriaceae (cytophaga-flavobacterium-bacteroides division). the strains were gram-negative, non-motile, psychrotolerant, strictly aerobic, chemoheterotrophic rod-shaped cells that contained orange or yellow carotenoid pigments and required yeast extract when grown in defined mineral-salts media. the requir ... | 2002 | 12361255 |
| phylogenetic analysis of the human gut microbiota using 16s rdna clone libraries and strictly anaerobic culture-based methods. | the human gut microbiota from three healthy subjects were compared by the use of a sequence analysis of 16s rdna libraries and a culture-based method. direct counts ranged from 1.9 x 10" to 4.0 x 10" cells/g (wet weight), and plate counts totaled 6.6 x 10(10) to 1.2 x 10(11) cfu/g (wet weight). sixty to seventy percent of the bacteria in the human intestinal tract cannot be cultured with currently available methods. the 16s rdna libraries from three subjects were generated from total community d ... | 2002 | 12363017 |
| extra- and intra-cellular lytic effects of cytophaga sp. lr2 on the red microalgae rhodella reticulata. | to evaluate the lytic activities of crude enzymes from cytophaga sp. lr2 on rhodella reticulata cells and isolated algal polysaccharide. | 2002 | 12392519 |
| thermostable aldehyde dehydrogenase from psychrophile, cytophaga sp. kuc-1: enzymological characteristics and functional properties. | we found the occurrence of nad(p)(+)-dependent aldehyde dehydrogenase (ec1.2.1.5) in the cells of a psychrophile from antarctic seawater, cytophaga sp. kuc-1, and purified to homogeneity. about 50% of the enzyme activity remained even after heating at 50 degrees c for 65min and the highest activity was observed in the range of 55-60 degrees c. the enzyme was thermostable and thermophilic, although it was derived from a psychrophile. the circular dichroism at 222nm of the enzyme showed a peak at ... | 2002 | 12419301 |
| analysis of 16s libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. | total genomic dna from samples of intact mouse small intestine, large intestine, caecum and faeces was used as template for pcr amplification of 16s rrna gene sequences with conserved bacterial primers. phylogenetic analysis of the amplification products revealed 40 unique 16s rdna sequences. of these sequences, 25% (10/40) corresponded to described intestinal organisms of the mouse, including lactobacillus spp., helicobacter spp., segmented filamentous bacteria and members of the altered schaed ... | 2002 | 12427955 |
| pharacine, a natural p-cyclophane and other indole derivatives from cytophaga sp. strain am13.1. | from the ethyl acetate extract of the bacterial strain cytophaga sp. am13.1, among many known compounds, the new natural products 2,5-bis(3-indolylmethyl)pyrazine (2) and a highly symmetrical p-cyclophane named pharacine (5) were identified. in addition, tryptamine isovalerate (1) and p-hydroxyphenylacetamide (4), known as plant metabolites, were isolated and characterized from a microorganism for the first time. the new natural products showed no activity against three microalgae, the fungus mu ... | 2002 | 12444694 |
| competitive pcr for quantitation of a cytophaga-flexibacter-bacteroides phylum bacterium associated with the tuber borchii vittad. mycelium. | an uncultured bacterium associated with the ectomycorrhizal fungus tuber borchii vittad. was identified as a novel member of the cytophaga-flexibacter-bacteroides group. utilizing a quantitative pcr targeting the 16s rrna gene, we relatively quantified this bacterium in the host. the estimated number of bacteria was found to be approximately 10(6) cells per 30-day-old t. borchii mycelium culture. this represents the first molecular attempt to enumerate an uncultured bacterium associated with a m ... | 2002 | 12450871 |
| impact of biocontrol strain pseudomonas fluorescens cha0 on rhizosphere bacteria isolated from barley (hordeum vulgare l.) with special reference to cytophaga-like bacteria. | to assess the impact of the biocontrol strain pseudomonas fluorescens cha0 on a collection of barley rhizosphere bacteria using an agar plate inhibition assay and a plant microcosm, focusing on a cha0-sensitive member of the cytophaga-like bacteria (clb). | 2002 | 12452964 |
| rapid detection of columnaris disease in channel catfish (ictalurus punctatus) with a new species-specific 16-s rrna gene-based pcr primer for flavobacterium columnare. | a 16-s rrna gene from the chromosomal dna of the fish-pathogenic bacterium flavobacterium columnare (formerly flexibacter columnaris), strain ars-i, was cloned, sequenced and used to design a polymerase chain reaction (pcr) primer set. the primer set amplified a specific 1193-bp dna fragment from f. columnare strains but not from related bacteria, f. psychrophilum, f. aquatile, f. branchiophilum, or other bacterial pathogens of fish, flexibacter maritimus, cytophaga johnsonae, edwardsiella ictal ... | 2003 | 12459241 |
| a simple method for dna extraction from marine bacteria that produce extracellular materials. | we present a simple method for extracting dna from the marine bacteria hahella chejuensis, a streptomyces sp., and a cytophaga sp. previously, dna purification from these strains was hindered by the presence of extracellular materials. in our extraction method, the marine bacteria are lysed by freezing and grinding in liquid nitrogen, and treated with sds. the extracted dna is purified using a phenol/chloroform mixture, and precipitated in isopropanol. the extracted dna is of high quality and su ... | 2003 | 12459245 |
| phylogenetic structure of the genera flexibacter, flexithrix, and microscilla deduced from 16s rrna sequence analysis. | the 16s rdna sequences of 40 strains of 17 species in the genus flexibacter, 5 strains of 4 species in the genus microscilla, and 1 strain of flexithrix dorotheae, including all type strains of approved and validated species in these genera, were determined to reveal their phylogenetic relationships. the 16s rrna sequence analysis demonstrated the extreme heterogeneity of the genera flexibacter and microscilla. the strains examined diverged into 24 distinct lines of descent (1 group included bot ... | 2002 | 12469298 |
| phylogenetic characterization of microbial mats and streamers from a japanese alkaline hot spring with a thermal gradient. | dense microbial mats and streamers of various colors developed in an alkaline-hot spring water at 48-76 degrees c and ~0.077 mm sulfide in nakabusa, japan. the microbial community structures with a thermal gradient were compared by denaturing gradient gel electrophoresis (dgge) analysis of the pcr-amplified 16s ribosomal rna gene fragments. the sequence analysis revealed that a predominant cyanobacterial dgge band phylogenetically related to synechococcus elongatus was detected only from green m ... | 2002 | 12469320 |
| microbial community structures in foaming and nonfoaming full-scale wastewater treatment plants. | a survey of full-scale activated-sludge plants in illinois revealed that filamentous foaming is a widespread problem in the state, and that the causes and consequences of foaming control strategies are not fully understood. to link microbial community structure to foam occurrence, microbial populations in eight foaming and nine nonfoaming full-scale activated-sludge systems were quantified using oligonucleotide hybridization probes targeting the ribosomal rna (rrna) of the mycolata; gordonia spp ... | 2002 | 12469948 |
| a preliminary report of phylogenetic diversity of bacterial strains isolated from marine creatures. | bacterial diversity among marine creatures, especially molluscs, as a source for searching out novel lineages of bacteria, was studied. marine creatures were collected at the coasts of the kanto area in japan. a total of 116 strains of bacteria were isolated from the intestines of 19 species of marine creatures includings molluscs, pisces and protochordata. partial sequencing of 16s rdna revealed that most of the isolates belonged to the gamma subclass of the proteobacteria and cytophaga-flavoba ... | 2002 | 12501435 |
| runella zeae sp. nov., a novel gram-negative bacterium from the stems of surface-sterilized zea mays. | a gram-negative bacterium, designated ns12(t), was previously isolated from duplicate treatments of surface-sterilized zea mays stems. the plants were grown in synthetic soil under greenhouse conditions and watered with fertilizer containing no nitrogen. strain ns12(t) was not isolated from plants watered with the standard level or 20% (w/v) of the standard level of nitrogen. cells were bent rods that formed chains of irregular shapes in r2a broth. unlike its closest described relative runella s ... | 2002 | 12508869 |
| biodegradation of an alicyclic hydrocarbon by a sulfate-reducing enrichment from a gas condensate-contaminated aquifer. | we used ethylcyclopentane (ecp) as a model alicyclic hydrocarbon and investigated its metabolism by a sulfate-reducing bacterial enrichment obtained from a gas condensate-contaminated aquifer. the enrichment coupled the consumption of ecp with the stoichiometrically expected amount of sulfate reduced. during ecp biodegradation, we observed the transient accumulation of metabolite peaks by gas chromatography-mass spectrometry, three of which had identical mass spectrometry profiles. mass-spectral ... | 2003 | 12514025 |
| a culture-independent survey of the bacterial community in a radon hot spring. | paralana is an active, radon-containing hot spring situated in a region of south australia's flinders ranges with a long history of hydrothermal activity. our aim was to determine the bacterial composition of paralana using a culture-independent, 16s rrna-based technique. the presence of a diverse bacterial community was strongly suggested by the large number (approximately 180) of different ribotypes obtained upon analysis of nine hot spring samples. dna sequencing of paralana 16s rrna genes co ... | 2002 | 12530236 |
| isolation and phylogenetic characterization of bacteria capable of inducing differentiation in the green alga monostroma oxyspermum. | many green algae cannot develop normally when they are grown under axenic conditions. monostroma oxyspermum, for example, proliferates unicellularly in an aseptic culture, but develops into a normal foliaceous gametophyte in the presence of some marine bacteria. more than 1000 bacterial strains were isolated from marine algae and sponges and assayed for their ability to induce the morphogenesis of unicellular m. oxyspermum. fifty bacterial strains exhibiting morphogenesis-inducing activity again ... | 2003 | 12542710 |
| [properties and mechanisms of marine polysaccharidases]. | | 2001 | 12552838 |
| depth distribution of microbial diversity in mono lake, a meromictic soda lake in california. | we analyzed the variation with depth in the composition of members of the domain bacteria in samples from alkaline, hypersaline, and currently meromictic mono lake in california. dna samples were collected from the mixolimnion (2 m), the base of the oxycline (17.5 m), the upper chemocline (23 m), and the monimolimnion (35 m). composition was assessed by sequencing randomly selected cloned fragments of 16s rrna genes retrieved from the dna samples. most of the 212 sequences retrieved from the sam ... | 2003 | 12571026 |
| diversity of 746 heterotrophic bacteria isolated from microbial mats from ten antarctic lakes. | microbial mats, growing in antarctic lakes constitute unique and very diverse habitats. in these mats microorganisms are confronted with extreme life conditions. we isolated 746 bacterial strains from mats collected from ten lakes in the dry valleys (lakes hoare and fryxell), the vestfold hills (lakes ace, druzhby, grace, highway, pendant, organic and watts) and the larsemann hills (lake reid), using heterotrophic growth conditions. these strains were investigated by fatty acid analysis, and by ... | 2002 | 12583721 |
| microbial community and physicochemical analysis of an industrial waste gas biofilter and design of 16s rrna-targeting oligonucleotide probes. | a study was conducted to investigate the microbial community structure, the physicochemical properties, and the relationships between these parameters of a full-scale industrial biofilter used for waste gas abatement in an animal-rendering plant. fluorescence in situ hybridization (fish) was successfully combined with digital image analysis to study the composition of the microbial community. several new nucleic acid probes were designed and established based on published 16s rdna sequences and ... | 2003 | 12588298 |
| use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil. | seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from antarctica. the column was maintained at 10 degrees c and continuously fed with a casein-containing medium to enrich protease producers. twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological a ... | 2003 | 12620829 |
| bacterial rrna genes associated with soil suppressiveness against the plant-parasitic nematode heterodera schachtii. | the goal of this study was to identify bacteria involved in soil suppressiveness against the plant-parasitic nematode heterodera schachtii. since h. schachtii cysts isolated from the suppressive soil can transfer this beneficial property to nonsuppressive soils, analysis of the cyst-associated microorganisms should lead to the identification of the causal organisms. our experimental approach was to identify bacterial rrna genes (rdna) associated with h. schachtii cysts obtained from soil mixture ... | 2003 | 12620845 |
| reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum cytophaga-flavobacterium-bacteroides. | a heterotrophic, pigmented, agarolytic, gliding bacterium was isolated from a seawater sample collected from the gulf of peter the great, sea of japan, during june 2000. 16s rdna sequence analysis indicated that the novel bacterium, strain kmm 3525t, was a member of the phlyum cytophaga-flavobacterium-bacteroides. on the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic data, it is proposed that the marine bacterium represents the sole species of a novel genus, reichenbachia, the t ... | 2003 | 12656156 |
| chryseobacterium defluvii sp. nov., isolated from wastewater. | a gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacterium (strain b2t) isolated from wastewater of a sequence batch reactor showing enhanced phosphorus removal was investigated to determine its taxonomic status. complete 16s rrna gene sequence analysis indicated that the organism should be placed in the genus chryseobacterium. the strain contained a polyamine pattern with sym-homospermidine as the major compound, menaquinone mk-6 as the predominant menaquinone and ai-c15:0, i-c1 ... | 2003 | 12656158 |
| high diversity among feather-degrading bacteria from a dry meadow soil. | the aim of this study was to determine the diversity of cultivable bacteria able to degrade feathers and present in soil under temperate climate. we obtained 33 isolates from soil samples, which clustered in 13 ardra groups. these isolates were able to grow on solid medium with pigeon feathers as sole carbon and nitrogen source. one representative isolate of each ardra group was selected for identification and feather degradation tests. the phylogenetic analysis of 16s rdna gene fragments reveal ... | 2003 | 12658523 |
| genetic profiling of noncultivated bacteria from the rhizospheres of sugar beet (beta vulgaris) reveal field and annual variability but no effect of a transgenic herbicide resistance. | in this field study, we compared the bacterial communities inhabiting the rhizosphere of a transgenic, herbicide-resistant sugar beet (beta vulgaris) cultivar with those of its nonengineered counterpart, using a genetic profiling technique based on pcr amplifications of partial 16s rrna gene sequences and single-strand conformation polymorphism (sscp). as a control for the plasticity of the bacterial community, we also analyzed the influence of herbicides, the field heterogeneity, and the annual ... | 2003 | 12674342 |
| extraordinary proliferation of microorganisms in aposymbiotic pea aphids, acyrthosiphon pisum. | aposymbiotic pea aphids, which were deprived of their intracellular symbiotic bacterium, buchnera, exhibit growth retardation and no fecundity. high performance liquid chromatographic (hplc) analysis revealed that these aposymbiotic aphids, when reared on broad bean plants, accumulated a large amount of histamine. to assess the possibility of extraordinary proliferation of microorganisms other than buchnera, we enumerated eubacteria and fungi in aphids using the real-time quantitative pcr method ... | 2003 | 12676551 |
| spatial and temporal analysis of the microbial community in slow sand filters used for treating horticultural irrigation water. | an experimental slow sand filter (ssf) was constructed to study the spatial and temporal structure of a bacterial community suppressive to an oomycete plant pathogen, phytophthora cryptogea. passage of water through the mature sand column resulted in complete removal of zoospores of the plant pathogen. to monitor global changes in the microbial community, bacterial and fungal numbers were estimated on selective media, direct viable counts of fungal spores were made, and the atp content was measu ... | 2003 | 12676691 |
| bacterioplankton community shifts in an arctic lake correlate with seasonal changes in organic matter source. | seasonal shifts in bacterioplankton community composition in toolik lake, a tundra lake on the north slope of alaska, were related to shifts in the source (terrestrial versus phytoplankton) and lability of dissolved organic matter (dom). a shift in community composition, measured by denaturing gradient gel electrophoresis (dgge) of 16s rrna genes, occurred at 4 degrees c in near-surface waters beneath seasonal ice and snow cover in spring. this shift was associated with an annual peak in bacteri ... | 2003 | 12676708 |
| improving the thermostability of raw-starch-digesting amylase from a cytophaga sp. by site-directed mutagenesis. | a heat-stable raw-starch-digesting amylase (rsda) was generated through pcr-based site-directed mutagenesis. at 65 degrees c, the half-life of this mutant rsda, which, compared with the wild-type rsda, lacks amino acids r178 and g179, was increased 20-fold. while the wild type was inactivated completely at ph 3.0, the mutant rsda still retained 41% of its enzymatic activity. the enhancement of rsda thermostability was demonstrated to be via a ca(2+)-independent mechanism. | 2003 | 12676725 |
| occupational lung disease related to cytophaga endotoxin exposure in a nylon plant. | workers at a nylon plant developed pulmonary disease with systemic symptoms. differentiating between humidifier fever and hypersensitivity pneumonitis (hp) is challenging. cytophaga, an endotoxin-producing bacteria, was isolated from the plant air-conditioning system. a number of workers had systemic and pulmonary symptoms. precipitins to cytophaga endotoxin were identified. several workers underwent lung biopsies demonstrating hp. inhalation challenges with purified cytophaga endotoxin were per ... | 2003 | 12708142 |
| specificity in the settlement -- modifying response of bacterial biofilms towards zoospores of the marine alga enteromorpha. | previous studies have shown that the rate of settlement of zoospores of the green alga enteromorpha is stimulated by mixed microbial biofilms and that the number of zoospores settling is positively correlated with the number of bacteria in the biofilm. in the present study the specificity of this relationship has been investigated. ninety-nine strains of marine bacteria were isolated from natural biofilms on rocks and the surface of enteromorpha plants. isolates were screened by denaturing gradi ... | 2003 | 12713460 |
| microbial diversity in a thermophilic aerobic biofilm process: analysis by length heterogeneity pcr (lh-pcr). | a two-stage pilot-scale thermophilic aerobic suspended carrier biofilm process (scbp) was set up for the on-site treatment of pulp and paper mill whitewater lining. the microbial diversity in this process was analyzed by length heterogeneity analysis of pcr-amplified 16s ribosomal dna. the primer pair selected for pcr amplification was first evaluated by a computational analysis of fragment lengths in ten main phylogenetical eubacterial groups. the fragment contained the first third of the 16s r ... | 2003 | 12727234 |
| are readily culturable bacteria in coastal north sea waters suppressed by selective grazing mortality? | we studied the growth of six culturable bacterial lineages from coastal north sea picoplankton in environmental samples under different incubation conditions. the grazing pressure of heterotrophic nanoflagellates (hnf) was reduced either by double prefiltration through 0.8- micro m-pore-size filters or by 10-fold dilutions with 0.2- micro m (pore-size) prefiltered seawater. we hypothesized that those gamma-proteobacterial genera that are rapidly enriched would also be most strongly affected by h ... | 2003 | 12732530 |
| bacterioplankton community structure in a maritime antarctic oligotrophic lake during a period of holomixis, as determined by denaturing gradient gel electrophoresis (dgge) and fluorescence in situ hybridization (fish). | the bacterioplankton community structure in moss lake, a maritime antarctic oligotrophic lake, was determined with vertical depth in the water column, during the ice-free period on signy island in the south orkney islands. bacterioplankton community structure was determined using a combination of direct counting of 4',6-diamidino-2-phenylindole (dapi) stained cells, pcr amplification of 16s rrna gene fragments, denaturing gradient gel electrophoresis (dgge) and in situ hybridization with group-s ... | 2003 | 12739078 |
| enumeration of bacteroides species in human faeces by fluorescent in situ hybridisation combined with flow cytometry using 16s rrna probes. | bacteroides is a predominant group of the faecal microbiota in healthy adults. to investigate the species composition of bacteroides by fluorescent in situ hybridisation (fish) combined with flow cytometry, we developed five species-specific probes targeting the 16s rrna. probes were designed to identify cells belonging to bacteroides distasonis, b. fragilis, b. ovatus, b. vulgatus and b. putredinis. the species-specificity of the probes was assessed against a collection of reference strains fro ... | 2003 | 12747418 |
| carriage of potentially fish-pathogenic bacteria in sparus aurata cultured in mediterranean fish farms. | a bacteriological survey of gilthead sea bream sparus aurata from different fish farms and culture systems on the spanish mediterranean coast was conducted. three different studies were performed. study a included hatchery-reared larvae; study b, periodic examination of randomly sampled growing fish; and study c, growing fish sampled only during mortality/morbidity events. in studies b and c, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical sign ... | 2003 | 12747637 |
| thermostable aspartase from a marine psychrophile, cytophaga sp. kuc-1: molecular characterization and primary structure. | we found that a psychrophilic bacterium isolated from antarctic seawater, cytophaga sp. kuc-1, abundantly produces aspartase [ec4.3.1.1], and the enzyme was purified to homogeneity. the molecular weight of the enzyme was estimated to be 192,000, and that of the subunit was determined to be 51,000: the enzyme is a homotetramer. l-aspartate was the exclusive substrate. the optimum ph in the absence and presence of magnesium ions was determined to be ph 7.5 and 8.5, respectively. the enzyme was act ... | 2003 | 12761198 |
| combining culture-dependent and -independent methodologies for estimation of richness of estuarine bacterioplankton consuming riverine dissolved organic matter. | three different methods for analyzing natural microbial community diversity were combined to maximize an estimate of the richness of bacterioplankton catabolizing riverine dissolved organic matter (rdom). we also evaluated the ability of culture-dependent quantitative dna-dna hybridization, a 16s rrna gene clone library, and denaturing gradient gel electrophoresis (dgge) to detect bacterial taxa in the same sample. forty-two different cultivatable strains were isolated from rich and poor solid m ... | 2003 | 12788769 |
| sugar metabolism in the extremely halophilic bacterium salinibacter ruber. | growth of salinibacter ruber, a red, extremely halophilic bacterium phylogenetically affiliated with the flavobacterium/cytophaga branch of the domain bacteria, is stimulated by a small number of sugars (glucose, maltose, starch at 1 g l(-1)). glucose consumption starts after other substrates have been depleted. glucose metabolism proceeds via a constitutive, salt-inhibited hexokinase and a constitutive salt-dependent nicotinamide adenine dinucleotide phosphate (nadp)-linked glucose-6-phosphate ... | 2003 | 12799004 |
| survivability and long-term preservation of bacteria in water and in phosphate-buffered saline. | to evaluate the suitability of using sterile water and phosphate-buffered saline (pbs) for preservation of bacteria pathogenic to plants or humans. | 2003 | 12803555 |
| biodegradation of mtbe and btex in an aerobic fluidized bed reactor. | an aerobic fluidized bed reactor (fbr) was operated for the removal of methyl tert-butyl (mbe) and benzene, toluene, ethylbenzene, and p-xylene (btex) from water. the reactor was seeded with a mixed culture adapted to mtbe. granular activated carbon (gac) was used as the biological attachment medium. influent mtbe to the reactor was 7.8 mg/l mtbe, with a flow rate of 22.7 l/day, and an empty bed contact time of 1 hour. the acclimation period required was relatively short, about 30 days before re ... | 2003 | 12830950 |
| heterotrophic bacterial growth efficiency and community structure at different natural organic carbon concentrations. | batch cultures of aquatic bacteria and dissolved organic matter were used to examine the impact of carbon source concentration on bacterial growth, biomass, growth efficiency, and community composition. an aged concentrate of dissolved organic matter from a humic lake was diluted with organic compound-free artificial lake water to obtain concentrations of dissolved organic carbon (doc) ranging from 0.04 to 2.53 mm. the bacterial biomass produced in the cultures increased linearly with the doc co ... | 2003 | 12839735 |
| identification of a new ribosomal protection type of tetracycline resistance gene, tet(36), from swine manure pits. | previously, only one ribosome protection type of a tetracycline resistance gene, tetq, had been identified in bacteroides spp. during an investigation of anaerobic bacteria present in swine feces and manure storage pits, a tetracycline-resistant bacteroides strain was isolated. subsequent analysis showed that this new bacteroides strain, bacteroides sp. strain 139, did not contain tetq but contained a previously unidentified tetracycline resistance gene. sequence analysis showed that the tetracy ... | 2003 | 12839793 |
| novel psychrophilic and thermolabile l-threonine dehydrogenase from psychrophilic cytophaga sp. strain kuc-1. | a psychrophilic bacterium, cytophaga sp. strain kuc-1, that abundantly produces a nad(+)-dependent l-threonine dehydrogenase was isolated from antarctic seawater, and the enzyme was purified. the molecular weight of the enzyme was estimated to be 139,000, and that of the subunit was determined to be 35,000. the enzyme is a homotetramer. atomic absorption analysis showed that the enzyme contains no metals. in these respects, the cytophaga enzyme is distinct from other l-threonine dehydrogenases t ... | 2003 | 12867457 |
| sulfate-reducing bacterial community structure and their contribution to carbon mineralization in a wastewater biofilm growing under microaerophilic conditions. | the community structure of sulfate-reducing bacteria (srb) and the contribution of srb to carbon mineralization in a wastewater biofilm growing under microaerophilic conditions were investigated by combining molecular techniques, molybdate inhibition batch experiments, and microelectrode measurements. a 16s rdna clone library of bacteria populations was constructed from the biofilm sample. the 102 clones analyzed were grouped into 53 operational taxonomic units (otus), where the clone distributi ... | 2003 | 12879306 |
| cloning and functional expression of dipeptidyl peptidase iv from the ruminal bacterium prevotella albensis m384(t). | ruminal bacteria of the genus prevotella play a crucial role in peptide breakdown in the rumen, a component of protein catabolism that leads to the inefficient use of dietary protein by ruminant animals. this is the first report of the cloning of a peptidase gene from a ruminal bacterium. part of the dipeptidyl peptidase type iv (dpp-iv) gene from prevotella albensis m384(t) was cloned using degenerate primers designed from conserved regions found within other known dpp-iv sequences. flanking re ... | 2003 | 12904562 |
| high 16s rdna bacterial diversity in glacial meltwater lake sediment, bratina island, antarctica. | the microbial diversity in maritime meltwater pond sediments from bratina island, ross sea, antarctica was investigated by 16s rdna-dependent molecular phylogeny. investigations of the vertical distribution, phylogenetic composition, and spatial variability of bacteria and archaea in the sediment were carried out. results revealed the presence of a highly diverse bacterial population and a significantly depth-related composition. assessment of 173 partial 16s rdna clones analyzed by amplified rd ... | 2003 | 12910387 |
| molecular characterization of the microbial species that colonize human ileal and colonic mucosa by using 16s rdna sequence analysis. | the aim of this study was to characterize the bacterial community adhering to the mucosa of the terminal ileum, and proximal and distal colon of the human digestive tract. | 2003 | 12911699 |
| application of comparative genomics in the identification and analysis of novel families of membrane-associated receptors in bacteria. | a great diversity of multi-pass membrane receptors, typically with 7 transmembrane (tm) helices, is observed in the eukaryote crown group. so far, they are relatively rare in the prokaryotes, and are restricted to the well-characterized sensory rhodopsins of various phototropic prokaryotes. | 2003 | 12914674 |
| isolation, characterization and comparison of bacteria from swine faeces and manure storage pits. | storage of swine manure is associated with the microbiological production of a variety of odorous chemicals including ammonia, organic acids and alcohols, and sulphides. although largely the product of microbiological activity, little is known about the microorganisms present in swine manure. in order to gain a better understanding of the types and activities of the microorganisms present, representative strains of microorganisms were isolated from faeces and stored manure slurry, identified, an ... | 2003 | 12919409 |
| complete genome sequence of the oral pathogenic bacterium porphyromonas gingivalis strain w83. | the complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium porphyromonas gingivalis strain w83, a major contributor to periodontal disease, was determined. whole-genome comparative analysis with other available complete genome sequences confirms the close relationship between the cytophaga-flavobacteria-bacteroides (cfb) phylum and the green-sulfur bacteria. within the cfb phyla, the genomes most similar to that of p. gingivalis are those of bacteroides thetaiotaom ... | 2003 | 12949112 |
| bacterial diversity and sulfur cycling in a mesophilic sulfide-rich spring. | an artesian sulfide- and sulfur-rich spring in southwestern oklahoma is shown to sustain an extremely rich and diverse microbial community. laboratory incubations and autoradiography studies indicated that active sulfur cycling is occurring in the abundant microbial mats at zodletone spring. anoxygenic phototrophic bacteria oxidize sulfide to sulfate, which is reduced by sulfate-reducing bacterial populations. the microbial community at zodletone spring was analyzed by cloning and sequencing 16s ... | 2003 | 12957951 |
| incidence of a new sex-ratio-distorting endosymbiotic bacterium among arthropods. | many intracellular micro-organisms are now known to cause reproductive abnormalities and other phenomena in their hosts. the endosymbiont wolbachia is the best known of these reproductive manipulators owing to its extremely high incidence among arthropods and the diverse host effects it has been implicated as causing. however, recent evidence suggests that another intracellular bacterium, a cytophaga-like organism (clo), may also induce several reproductive effects in its hosts. here, we present ... | 2003 | 12964989 |
| [the effect of maleic hydrazide on the development of azobacter and cytophaga]. | | 1952 | 12998276 |
| vitellibacter vladivostokensis gen. nov., sp. nov., a new member of the phylum cytophaga-flavobacterium-bacteroides. | a novel heterotrophic, yellow-orange-pigmented, non-motile, asporogenic, strictly aerobic, gram-negative, oxidase and catalase-positive bacterium kmm 3516(t) was isolated from the holothurian apostichopus japonicus collected from troitsa bay in the gulf of peter the great (sea of japan) during november 1997. 16s rdna sequence analysis revealed that strain kmm 3516(t) was a member of the family flavobacteriaceae: the dna g+c content of kmm 3516(t) was 41.3 mol%. major respiratory quinone was mk-6 ... | 2003 | 13130007 |
| [the trace element requirements of cellvibrio and cytophaga]. | | 1953 | 13170582 |
| enzymes involved in the utilization of carbohydrates by two strains of myxobacteria. | | 1954 | 13189439 |
| studies on cytophaga fermentans, n.sp., a facultatively anaerobic lower myxobacterium. | | 1955 | 13278504 |
| [significance of trace elements for cell vibrio and cytophaga species types]. | | 1956 | 13395442 |
| the morphology of a species of the bacterial genus cytophaga winog. in culture. | | 1957 | 13472515 |