the purification and some properties of h-lysin from aeromonas salmonicida. | h-lysin from aeromonas salmonicida has been purified 1770-fold by freeze fractionation, ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography. the purified material was predominantly h-lysin, devoid of detectable t-lysin, caseinase or gelatinase activity, although glycerophospholipid: cholesterol acyltransferase (gcat) activity was present. the results suggested that h-lysin and gcat activities were due to different extracellular products. studies of the ... | 1985 | 4045422 |
piracetam does not change the anaesthetic requirement for halothane. | mac of halothane was determined in rats before and after the injection of piracetam 0.5 or 2.5 g kg-1, or both. no consistent changes in mac were found. we conclude that piracetam is unlikely to influence halothane requirement appreciably. | 1985 | 4052302 |
a mouse model for entamoeba histolytica infection. | 2 strains of entamoeba histolytica, saw 760 and saw 408 (non-invasive zymodeme ix and invasive zymodeme ii respectively, sargeaunt & williams 1979) were administered intra-intestinally to 15 inbred and 3 outbred strains of mouse. all were known to be free of the mouse amoeba e. muris. during the investigation, all animals were maintained in plastic film isolator units, under gnotobiotic conditions. both zymodemes were found to be capable of infecting all 18 strains of mice for varying periods of ... | 1985 | 4068657 |
a comparison of the growth curves of the nq bacillus (mycobacterium sp.) derived by photometric turbidity, microscopic counting, and viability in a tube-dilution-series. | | 1971 | 4106082 |
detection of r factors in naturally occurring aeromonas salmonicida strains. | r factors were detected in aeromonas salmonicida strains isolated from diseased salmonid fish. one of such r(+) strains was the one isolated in the united states as early as 1959. | 1971 | 4108649 |
hydroperoxide peroxidase activity in liver microsomes. | | 1974 | 4152625 |
characterization of two different agglutinators in the latex fixation test, occurring in normal human sera. | | 1966 | 4160336 |
localization in the kidney of antibodies to human skin and malignant melanoma. | | 1966 | 4161935 |
virus disease in pregnancy. | | 1969 | 4236595 |
[studies on the variability of content and composition of volatile oil in leaf- and herb-drugs of thymus vulgaris l]. | | 1970 | 4245001 |
on the active site of myosin a-adenosine triphosphatase. x. functions of two subfragments, s-1, of the myosin molecule. | | 1970 | 4249516 |
[mosaic of g-g translocation observed in a girl with faint symptoms of mongolism]. | | 1971 | 4257569 |
the isolation of viruses from acute respiratory infections. v. the use of organ cultures of human embryonic nasal and tracheal ciliated epithelium. | | 1966 | 4292507 |
the isolation of viruses from acute respiratory infections. v. the use of organ cultures of human embryonic nasal and tracheal ciliated epithelium. | | 1966 | 4292507 |
effect of polynucleotides on aminoacyl transfer ribonucleic acid synthetases. 3. inhibition of glutamyl transfer ribonucleic acid synthetase by natural polynucleotides. | | 1968 | 4299520 |
the production and effect of interferon in organ cultures of calf trachea. | | 1968 | 4302497 |
response of hyperplastic alveolar nodule outgrowth-line d1 to mammary tumor virus, nodule-inducing virus, and prolonged hormonal stimulation acting singly and in combination. | | 1969 | 4303882 |
simian virus 40 transformation and the period of cellular deoxyribonucleic acid synthesis. | the antiviral agents interferon and statolon protected cells of the mouse line 3t3 against the transforming effect of simian virus 40. loss of ability of these agents to protect when added some time after infection indicated that the transformation was already fixed. the cells of exponentially growing cultures became resistant to the protective effect of interferon at a linear rate after infection; after one cell generation, the whole population was resistant. by use of synchronous cultures, it ... | 1967 | 4318942 |
simian virus 40 transformation and the period of cellular deoxyribonucleic acid synthesis. | the antiviral agents interferon and statolon protected cells of the mouse line 3t3 against the transforming effect of simian virus 40. loss of ability of these agents to protect when added some time after infection indicated that the transformation was already fixed. the cells of exponentially growing cultures became resistant to the protective effect of interferon at a linear rate after infection; after one cell generation, the whole population was resistant. by use of synchronous cultures, it ... | 1967 | 4318942 |
sarcoma blockade in vivo. rabies-rous system in chickens. | | 1970 | 4320567 |
determination of the gene sequence of poliovirus with pactamycin. | by examination of the virus-specific polypeptides formed after the addition of pactamycin, an inhibitor of protein chain initiation, to infected cells, it has been possible to tentatively locate the virus coat proteins at the amino terminus of the large, virus-specific protein precursor, and, therefore, to assign the coat protein cistron to the 5' end of the rna. | 1971 | 4330946 |
avian leukosis-sarcoma virus antibodies in wildfowl, domestic chickens and man in kenya. | | 1973 | 4358887 |
cardiovascular effects of pancuronium in man. | | 1971 | 4396862 |
simple method of 24-hour spleen preservation: further studies relating to hemophilia. | | 1971 | 4398505 |
simple method of 24-hour spleen preservation: further studies relating to hemophilia. | | 1971 | 4398505 |
intravenously administered doxycycline in urological and surgical infections. | | 1972 | 4402952 |
pseudomonas cepacia in the west of scotland. | | 1974 | 4418941 |
[bacteriological diagnosis of aeromonas salmonicida infections in fishes]. | | 1974 | 4441360 |
proceedings: the reversibility of the isomerization of the delta 8 to delta 7 bond in cholesterol biosynthesis. | | 1974 | 4443210 |
[current problems in bcg vaccination]. | | 1974 | 4445318 |
digestion of autologous igg by acid lysosomal protease (cathepsin d) and its role in immune complex formation and inflammation. | | 1974 | 4468717 |
toxicity of metrizamide and metrizoate in selective coeliac angiography. an experimental investigation in rabbits. | | 1973 | 4534552 |
toxicity of metrizamide and metrizoate in selective coeliac angiography. an experimental investigation in rabbits. | | 1973 | 4534552 |
[homage to jirovec. criticism of the taxonomic position of pneumocystis carinii]. | | 1972 | 4539134 |
[detection of enterococci on plants]. | | 1973 | 4589037 |
persistence and dissemination in the community of r-factors of nosocomial origin. | | 1974 | 4590005 |
partial similarities between yeast and liver alcohol dehydrogenases. | the primary structure of about half of the protein chain of yeast alcohol dehydrogenase has been determined and compared with the amino-acid sequences of other dehydrogenases. the enzyme is found to be distantly related to horse-liver alcohol dehydrogenase, although these two proteins have different quaternary structures and subunit sizes. some regions show no significant similarities, but long segments within the n-terminal parts of the molecules are homologous, suggesting a common and importan ... | 1973 | 4599620 |
[brief review on assessment of myocardial contractility (author's transl)]. | | 1974 | 4614352 |
identical increases in blood sialic acid of dogs given actinomycin d and dexamethasone. | | 1972 | 4641216 |
acute effect of neural deafferentation on timing of gonadotropin secretion before proestrus in the female rat. | | 1972 | 4678854 |
effect of various carbohydrates in a repletion diet after protein depletion on liver lipid content of rats. | | 1973 | 4684357 |
biological activity of proinsulin and related polypeptides in the fat tissue. | | 1973 | 4708090 |
interaction of estradiol and testosterone in the regulation of growth and development of the chicken (gallus domesticus) oviduct. | | 1973 | 4709979 |
mutations affecting meiosis in podospora anserina. ii. effect of mei2 mutants on recombination. | | 1973 | 4731428 |
mutations affecting meiosis in podospora anserina. ii. effect of mei2 mutants on recombination. | | 1973 | 4731428 |
stimulation of growth of new axonal sprouts from lesioned monoamine neurones in adult rat brain by nerve growth factor. | | 1973 | 4744759 |
a hypocalcemic agent containing gastrin from distal portions of the stomach in man and in the rat. | | 1974 | 4808689 |
characterization of anti-aeromonas salmonicida antibodies from coho salmon. | coho salmon (oncorhynchus kisutch) anti-aeromonas salmonicida agglutinins were excluded from sephadex g-200 and their activity was lost after partial reduction and alkylation followed by exposure to 2 m urea. immunoelectrophoretic analysis of coho antiserum revealed anodic and cathodic populations of immunoglobulin (ig), and the anti-a. salmonicida antibodies present 12 weeks after parenteral immunization were primarily anodic ig. antibodies were specifically purified by dissociation of a. salmo ... | 1974 | 4816458 |
proceedings: determination of ovulation time in the domestic fowl by radio-telemetry. | | 1974 | 4819351 |
pathogenesis for viral infections of the nervous system. | | 1968 | 4870529 |
partial reactivation of irradiated phage p1 by strain bs2 of escherichia coli. | | 1968 | 4880558 |
fluorescent antibody studies of isolates of aeromonas salmonicida. | | 1968 | 4888717 |
pituitary activation by bacterial endotoxins in rainbow trout (salmo gairdneri). | endotoxins from escherichia coli and aeromonas salmonicida caused marked cortisol production in the rainbow trout (salmo gairdneri). | 1969 | 4899008 |
purification from calf thymus of an inhibitor of deoxyribonuclease i. | | 1970 | 4909306 |
the use of zonal centrifugation in the preparation of glomeruli and tubular fragments from rat kidney. | | 1971 | 4940378 |
[survey of entero-infections in urban and rural areas of the algerois]. | | 1970 | 4950343 |
[survey of entero-infections in urban and rural areas of the algerois]. | | 1970 | 4950343 |
[measure of the sub orbital torus of human fossils]. | | 1967 | 4964610 |
purification and characterization of an immunologically active glycoprotein from asperigillus fumigatus. | | 1968 | 4969607 |
[activation by heat of bacterial enzymes acting on phospholipids]. | | 1969 | 4975881 |
[bacteriophages and lysotyping of aeromonas salmonicida]. | | 1970 | 4987117 |
physical, chemical, and serological studies on carnation mottle virus. | | 1970 | 4991706 |
collie eye anomaly. | | 1971 | 4994718 |
[proteins and enzymes of fetal blood and liver following administration of the teratogenic drug chloridine to pregnant rats]. | | 1972 | 5013001 |
lipid metabolism of human brain tumours. | | 1972 | 5054536 |
morphometry of structures in choroid plexus of man and some vertebrates. | | 1972 | 5066257 |
[transferable resistance factors in aeromonas salmonicida]. | | 1971 | 5129958 |
[on the taxonomy of aeromonas salmonicida subsp. achromogenes (smith 1963) schubert 1967 and aeromonas salmonicida subsp. masoucida kimura 1969]. | | 1969 | 5386249 |
the pathogenicity of aeromonas salmonicida (griffin) in sea and brackish waters. | | 1968 | 5640232 |
serological studies on proteinases produced by aeromonas salmonicida and other aeromonads. | | 1968 | 5653618 |
[serologic studies on aeromonas salmonicida]. | | 1964 | 5833568 |
isolation of an anaerogenic bacterium resembling aeromonas salmonicida in spawning lake trouts. | | 1966 | 6007060 |
the effect of primary and secondary immunization on the lymphoid tissues of the carp, cyprinus carpio l. | mirror carp immunized with human gamma globulin (hgg) in freund's complete adjuvant (fca) show a proliferative response involving cells whose cytoplasm stains deep red with methyl green-pyronin (pyroninophilic cells). this response occurs particularly in the haemopoietic parenchyma of the pronephros and mesonephros. it peaks at week 3, with the formation of clusters of pyroninophilic cells in the pronephros. immunization with aeromonas salmonicida elicited a less intense pyroninophilic response ... | 1982 | 6177828 |
structural studies on the o-antigen of aeromonas salmonicida. | lipopolysaccharide from a strain of aeromonas salmonicida salmonicida was isolated from cells by the aqueous phenol method in 2.3% yield (based on dry weight of bacteria). hydrolysis of the lipopolysaccharide in 1% acetic acid afforded o-polysaccharide (19% by weight), core-oligosaccharide (12.2%) and lipid a (44.6%). analysis indicated that 3-deoxy-d-manno-2-octulosonic acid was absent from the lipopolysaccharide and that no low-molecular-weight compounds were released by the mild hydrolysis. t ... | 1983 | 6188611 |
induction of nonspecific immunosuppression in a primitive teleost, rainbow trout (salmo gairdneri). | induction of immunological non-reactivity to goldfish red blood cells (gfrbc), sheep red blood cells (srbc) and to formalin-killed aeromonas salmonicida cells was obtained in yearling rainbow trout raised under laboratory aquarium conditions (at 10 degrees c). haemolysin and agglutinin titres were measured 1 to 4 weeks after immunization. while trout immunized with low and high doses of antigen by intramuscular (im) and intraperitoneal (ip) injections produced high antibody titres, administratio ... | 1984 | 6238855 |
purification and reconstitution in lipid bilayer membranes of an outer membrane, pore-forming protein of aeromonas salmonicida. | we have purified a major outer membrane protein from aeromonas salmonicida. this 42-kilodalton protein shared several physical characteristics with enterobacterial porins in that it was noncovalently associated with the peptidoglycan, it was released from the peptidoglycan in the presence of 0.1 m nacl and sodium dodecyl sulfate, and its mobility on sodium dodecyl sulfate-polyacrylamide gels was dependent on the solubilization temperature before electrophoresis. when added to the aqueous solutio ... | 1983 | 6315674 |
purification and characterization of a new proteolytic enzyme produced by aeromonas salmonicida. | a proteolytic enzyme produced by the fish pathogen aeromonas salmonicida was isolated and purified. it showed the following characteristics: temperature optimum at 48 degrees c, ph optimum at ph 9 and a molecular weight of 87500. the enzyme was inhibited by phenylmethanesulphonylfluoride (pmsf) indicating it to be a serine protease. | 1983 | 6343335 |
[study of various application methods for active immunization of fishes with aeromonas salmonicida, aeromonas punctata and vibrio anguillarum. 1. immunization with aeromonas salmonicida strain 314]. | | 1983 | 6352234 |
structural and immunochemical homogeneity of aeromonas salmonicida lipopolysaccharide. | sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides of typical and atypical strains of the fish pathogen aeromonas salmonicida. 32p intrinsically radiolabeled lipopolysaccharide in sarcosinate-extracted outer membrane preparations, lipopolysaccharide stained by silver in proteinase k-digested outer membrane preparations and whole cell lysates, as well as purified lipopolysaccharide, displayed o-polysaccharide chains which were unusually homogeneo ... | 1984 | 6370955 |
furunculosis in rainbow trout (salmo gairdneri) raised in sea water. | ulcerative skin lesions were encountered in rainbow trout raised in sea water by a commercial concern in the western cape, south africa. grossly, the lesions resembled furunculosis but, histopathologically, they differed from typical furunculosis in that bacterial colonies were rarely found in the organs, and also the kidneys and spleens were minimally involved. the causative organism was identified as an achromogenic aeromonas salmonicida that shared characteristics with all 3 subspecies, salmo ... | 1984 | 6493730 |
surface layer virulence a-proteins from aeromonas salmonicida strains. | superficial surface layer proteins (a-proteins) were present on diverse isolates of aeromonas salmonicida which differed both physiologically and in pathogenesis. three of these proteins were purified directly from the surface of whole cells or from outer membrane preparations. these a-proteins were unusually hydrophobic (45-47%) and of similar but not identical molecular mass (49, 50, and 51 kdaltons). they were nearly identical in amino acid composition and were highly conserved, but not ident ... | 1984 | 6525563 |
phylogenetic relationships among vibrio anguillarum plasmids. | results of restriction endonuclease analysis and southern blot hybridization suggest that the r-plasmids from vibrio anguillarum strains isolated in japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. molecular cloning experiments allowed identification of specific restriction endonuclease fragments carrying the genes for either cmr or tcr as the common sequences between some of these groups of r-plasmids. the cmr region fr ... | 1984 | 6528000 |
acquisition of iron by aeromonas salmonicida. | the ability of six typical and three atypical strains of aeromonas salmonicida to sequester fe3+ from the high-affinity iron chelators ethylenediaminedihydroxy-phenylacetic acid, lactoferrin, and transferrin was determined. typical strains were readily able to sequester fe3+ and used two different mechanisms. one mechanism was inducible and appeared to involve production of a low-molecular-weight soluble siderophore(s). iron uptake by this mechanism was strongly inhibited by ferricyanide. one vi ... | 1983 | 6630151 |
a lipopolysaccharide-specific bacteriophage for aeromonas salmonicida. | cell wall lipopolysaccharide (lps) was identified as the receptor for the aeromonas salmonicida bacteriophage strain 55r-1. mutants of a. salmonicida resistant to phage 55r-1 were unable to adsorb phage 55r-1 and were shown to be defective in lps structure. purified a. salmonicida lps inactivated phage 55r-1, but the o-polysaccharide and the core oligosaccharide portions of the lps were ineffective. these results suggest that lipid a was required for receptor activity. antibodies directed agains ... | 1983 | 6661705 |
enhancement of bacterial adhesion by shear forces: characterization of the haemagglutination induced by aeromonas salmonicida strain 438. | application of a viscometric assay to the haemagglutination induced by aeromonas salmonicida strain 438 showed that shear forces can enhance the strength of bacterial adhesion. the d-mannose/l-fucose-sensitive reaction proceeded in two phases, an initial phase in which the degree of aggregation remained constant during shearing and a second stage, induced by shear, in which agglutination was enhanced as shear was maintained. the results strongly paralleled those found in studies of concanavalin ... | 1983 | 6668467 |
characterization of plasmids in bacterial fish pathogen. | plasmid profiles of representative fish pathogens, aeromonas salmonicida, aeromonas hydrophila, vibrio anguillarum, pasteurella piscicida, yersinia ruckeri, edwardsiella tarda, and renibacterium salmoninarum, were determined by agarose gel electrophoresis with four different plasmid detection methods. a combination of two methods was required to detect the plasmids present in these strains and to calculate precisely the molecular weights of the plasmids. of 38 strains, 28 harbored one or more pl ... | 1983 | 6822413 |
purification and characterization of the cell surface virulent a protein from aeromonas salmonicida. | the predominant cell surface protein (a protein) of aeromonas salmonicida has been purified in high yield from outer membranes by using a combination of detergent and chaotropic extraction methods as well as exclusion and ion-exchange chromatography. the a protein was primarily monomeric, mr 50 000, but readily formed oligomers at high protein or low salt concentrations. several isoelectric forms were distinguishable with purified protein as well as in situ on the cell surface. neither phosphate ... | 1983 | 6871174 |
fish biologics: vaccine standards for sport fisheries and commercial aquaculture. | biologics for serodiagnostic identification of fish diesease agents and for determination of efficacy and potency of fish bacterins are now being offered on a limited basis to governmental hatchery biologists and commercial aquaculture farms by the national fish health research laboratory - leetown. difficulties arise when biologics are used for determining standard parameters for fish bacterins because there may be variations in the fishes' immune response due to population differences or effec ... | 1980 | 6995216 |
[direct immunofluorescence in the diagnosis of aeromonas salmonicida infections in fresh water fishes]. | | 1981 | 7018491 |
cell surface of the fish pathogenic bacterium aeromonas salmonicida. i. relationship between autoagglutination and the presence of a major cell envelope protein. | a comparison was made of membrane protein patterns of various aeromonas salmonicida strains, initially isolated from different habitats with respect to fish species affected, pathological entity, and geographic location of the outbreak of the disease. a major protein with a molecular weight of 54 000 was found in all autoagglutinating strains, whereas this protein is present in low amounts, or not at all, in non-autoagglutinating strains. evidence for a causal relationship between the presence o ... | 1982 | 7055565 |
cell surface of the fish pathogenic bacterium aeromonas salmonicida. ii. purification and characterization of a major cell envelope protein related to autoagglutination, adhesion and virulence. | the purification of the major protein of the membrane fraction of an autoagglutinating strain of aeromonas salmonicida is described. this protein, designated as additional cell envelope protein, is water-insoluble, has a molecular weight of about 54 000 and its amino terminal sequence is h2n-asp-val-leu-leu. neither sulphur-containing amino acids nor sugar residues were detected. its amino acid composition, which shows that the additional cell envelope protein is hydrophobic in nature, is remark ... | 1982 | 7055566 |
purification and partial characterization of a bacterial phospholipid: cholesterol acyltransferase. | a glycerophospholipid:cholesterol acyltransferase has been purified to near homogeneity from cell-free culture supernatants of aeromonas salmonicida. the characteristics of the enzyme distinguish it from bacterial phospholipases; however, it shares several properties with the lecithin:cholesterol acyltransferase of mammalian plasma. thus, the enzyme inhibits 2-positional specificity as an acyltransferase and it will act as a phospholipase a2 in the absence of cholesterol. furthermore, it has no ... | 1982 | 7061477 |
role of surface components in serum resistance of virulent aeromonas salmonicida. | the ability of virulent strains of aeromonas salmonicida to resist the bactericidal activity of serum was quantitated. the a. salmonicida strains tested included virulent strains, mutants lacking the major surface a-protein, and mutants lacking a-protein and having a modified lipopolysaccharide structure. the sera evaluated included normal human, rabbit, and trout sera, immune trout serum, and immune rabbit serum containing antibodies to a-protein and lipopolysaccharide. virulent strains of a. s ... | 1982 | 7095843 |
purification and disposition of a surface protein associated with virulence of aeromonas salmonicida. | virulent strains of aeromonas salmonicida observed by electron microscopy were characterized by an outer layer exhibiting a tetragonal repeat pattern. attenuated strains had a 2.5 x 10(3)- to 5 x 10(3)-fold reduction in virulence and lost the outer layer, autoaggregating properties, and a 49-kilodalton protein (a protein) simultaneously. the a protein is the major protein component of outer membrane fractions of virulent strains. a variety of radiolabeling studies showed that this protein was su ... | 1981 | 7275932 |
loss of virulence during culture of aeromonas salmonicida at high temperature. | the effect of growth temperature on the loss of virulence of the fish pathogen aeromonas salmonicida was investigated. three virulent strains were grown in trypticase soy broth at temperatures ranging from 22 to 30 degrees c. growth at a higher-than-optimal temperature (26 to 27 degrees c for the three strains studied) resulted in the selection of spontaneous attenuated derivatives in the initial bacterial population. for example, virulent bacteria represented less than 10% of the population of ... | 1981 | 7287625 |
persistence of the virulence of aeromonas salmonicida strains kept in river sediments. | the persistence of pathogenicity in two strains of aeromonas salmonicida maintained in river sediments was studied at different times. cultures were kept about nine months, and the virulence was compared to its initial value exhibited by the same strain, subcultivated in rainbow trout fingerlings. trials involved intramuscular injection of comparable doses of bacteria to batches of 30 fish held at 15 degrees c, and recording of subsequent mortalities. no difference was found during the first six ... | 1980 | 7337394 |
relationships between selected strains of typical and atypical aeromonas salmonicida, aeromonas hydrophila, and haemophilus piscium. | serological analyses of typical and atypical aeromonas salmonicida divided the representative strains into two distinguishable groups based on their antigenic composition. however, the serological groupings do not coincide with divisions constructed on the basis of physiological reactions. all of the a. salmonicida strains and the two a. hydrophila strains examined share at least one antigen and are sensitive to a. salmonicida bacteriophage 40 rr2.8t. these data reinforce the reasons for placing ... | 1980 | 7397604 |
enterotoxigenicity of aeromonas strains in suckling mice. | the enterotoxigenicity of 170 aeromonas strains isolated from different sources (food poisoning, random food sampling, water, faeces) was examined by the suckling mouse test. the strains were grown on syncaye culture medium covered with sterilized membrane for kiil-kidney. the culture supernatants were inoculated orally. ileal loop dilatation was compared to that produced by the international standard enterotoxic escherichia coli b7a (o148 : h28) and b2c (o6 : h16) strains. of the 87 aeromonas h ... | 1980 | 7415872 |
identification and characterization of outer membrane fragments released by aeromonas sp. | aeromonas hydrophila and aeromonas salmonicida were shown to release "blebs" or fragments averaging 20-30 nm in diameter. the protein composition of the fragments was very similar to that of the corresponding outer membrane by sodium dodecyl sulphate--polyacrylamide gel electrophoresis and the fragments were shown to contain phospholipid and lipopolysaccharide. the results therefore indicate that the blebs are derived from the outer membrane of these organisms. fragments isolated directly by dif ... | 1980 | 7459670 |
recombinant infectious hematopoietic necrosis virus and viral hemorrhagic septicemia virus glycoprotein epitopes expressed in aeromonas salmonicida induce protective immunity in rainbow trout (oncorhynchus mykiss). | fragments of the glycoprotein genes of viral hemorrhagic septicemia virus (vhsv) and infectious hematopoietic necrosis virus (ihnv) were cloned into a bacterial broad-host-range expression vector under the control of the plac promoter. western blot (immunoblot) analysis with monoclonal antibodies specific to the glycoproteins demonstrated the inducible expression of the fusion proteins in escherichia coli. aeromonas salmonicida is the causative agent of furunculosis in salmonid fish. it was conf ... | 1995 | 7486994 |