| human papillomavirus transmission and carcinogenesis. | | 1990 | 2169235 |
| [diagnosis and follow-up study of human papillomavirus infection in benign epithelium and mild dysplasia of the uterine cervix]. | in this study, we evaluated the sensitivity of cytology, colposcopy and the dot blot method (vira pap, vira type) for the screening of hpv infection in benign epithelium and mild dysplasia of the uterine cervix, and then investigated subsequent changes in hpv dna in the lesion. the hpv infection detection rates for cytology, colposcopy and the dot blot method were 14/46 (30.4%), 30/46 (65.2%) and 35/46 (76.1%), respectively. cases where hpv dna was negative with the dot blot method but hpv infec ... | 1990 | 2169511 |
| biotin and phosphorus-isotopic labelled dna/rna probes for the detection of human papilloma virus sequences. | in this study, the diagnostic accuracy and practicability of different hybridization techniques for the detection of human papilloma virus (hpv) dna were tested. cervical cell scrapes (n = 67) were analysed for hpv-dnas 6/11 and 16, in order to compare a commercially available in situ dna hybridization-assay with the conventional southern-blot analysis. the in situ dna hybridization-assay gave a sensitivity of 81.5%, a specificity of 97.5% and a diagnostic efficiency of 91.0% for hpv-dnas 6/11. ... | 1990 | 2169684 |
| condylomata acuminata in children: frequent association with human papillomaviruses responsible for cutaneous warts. | to identify the papillomavirus types associated with condylomata acuminata in children and to evaluate their mode of transmission, we studied 32 children with anogenital warts. external condylomata were found in 12 of their mothers and in 10 of their fathers. ten mothers, including two without external lesions, had cervical condylomata. blot hybridization studies disclosed a genital human papillomavirus (hpv) in 14 of 27 children (hpv-6 in 12 and hpv-11 in two) and in 8 of 14 patients (hpv-6 in ... | 1990 | 2170467 |
| oral focal epithelial hyperplasia removed with co2 laser. | a case of oral focal epithelial hyperplasia (feh) treated with co2 laser surgery is presented. histological diagnosis is discussed. the association of human papillomavirus (hpv) type 32 with the lesions is demonstrated with dna in situ hybridization technique. laser surgery is suggested as a treatment of choice. | 1990 | 2170540 |
| [study on the localization of human papillomavirus dna in the vulvar disease by means of in situ hybridization]. | we investigated human papillomavirus (hpv) dna of vulvar diseases obtained from 18 cases of condyloma acuminatum, 3 cases of hyperplastic dystrophy without atypia, 4 cases of lichen sclerosus, 4 cases of bowenoid papulosis, one case of bowen disease and 8 cases of squamous cell cancer by in situ hybridization with biotinylated hpv 6, 11, 16 and 18 dna probes. the results of in situ hybridization analysis showed that hpv 6/11 was positive in 94.4% (17/18) of condyloma acuminatum. in 17 cases of h ... | 1990 | 2170544 |
| detection of human papillomavirus-genomic dna in oral epithelial dysplasias, oral smokeless tobacco-associated leukoplakias, and epithelial malignancies. | human papillomavirus (hpv) is an infectious agent that is increasingly associated with mucosal cancers, in particular cancer of the cervix. the present investigation was undertaken in an attempt to determine whether hpv could be easily detected in biopsies of oral tissues, specifically oral squamous cell carcinomas, oral epithelial dysplasias, smokeless tobacco keratoses, verrucous hyperplasia, and verrucous carcinoma. in situ dna hybridization methods were used to isolate specific hpv genomes. ... | 1990 | 2170606 |
| [the role of human papillomaviruses among the risk factors for cervical carcinoma]. | in a prospective study, risk factors of the development of cervical carcinoma in 210 diseased and 100 healthy subjects were investigated. special attention was paid to metaplastic changes in the portio vaginalis cervics and to human papilloma virus which are considered responsible for the development of these changes. it has been found that metaplasias are favoured by adolescence, chronic irritative processes in the cervix (pathologic discharge recorded in 51.9% of the diseased and 38% of the he ... | 1990 | 2170775 |
| the e7 protein of human papillomavirus 8 is a nonphosphorylated protein of 17 kda and can be generated by two different mechanisms. | the e7 protein of human papillomavirus (hpv) 8 shows no in vitro transforming activity, in contrast to e7 of the genital hpvs, but seems to be involved in the control of viral dna replication. to determine whether functional differences between the e7 proteins of hpv16 and hpv8 were reflected in differences in their biochemical properties, we characterized the e7 protein of hpv8 expressed from the late simian virus 40 promoter in cos 7 cells. an e7-specific antiserum was obtained by immunizing r ... | 1990 | 2171214 |
| carbon dioxide laser energy disperses human papillomavirus deoxyribonucleic acid onto treatment fields. | the possibility of dispersing viral deoxyribonucleic acid during carbon dioxide laser treatment of human papillomavirus-containing genital infections has been investigated with a commercially available dot blot hybridization technique. the viral ribonucleic acid probes were specific for groups of human papillomavirus types 6/11, 16/18, and 31/33/35. laser energy was delivered by continuous-wave mode and the plume of smoke was evacuated by a vacuum suction system. samples were taken with dacron s ... | 1990 | 2171340 |
| the presence of human papillomavirus (hpv) in solitary adult laryngeal papillomas demonstrated by in-situ dna hybridization with sulphonated probes. | human papilloma virus (hpv) types 6 and 11 have been repeatedly demonstrated in multiple laryngeal papillomas, and there is little doubt that these lesions are caused by hpv. it has been clearly demonstrated in recent reports that the clinical course of solitary adult onset laryngeal papillomas is entirely different from that of multiple papillomas of juvenile as well as of adult onset. we here report the presence of hpv types 6 and 11 in 19 out of 20 solitary papillomas from 16 patients, while ... | 1990 | 2171818 |
| human cervical cells immortalized in vitro with oncogenic human papillomavirus dna differentiate dysplastically in vivo. | human papillomavirus (hpv) types 16 and 18 are associated with cervical dysplasia and carcinoma. in vitro integration of hpv-16 or hpv-18 dna into cultured human cervical cells results in their immortalization. in this study, in vivo differentiation of human cervical cells immortalized with recombinant hpv-16 or hpv-18 dna was studied by implanting confluent monolayers of hpv-16 and hpv-18 dna immortalized cell lines under dorsal skin flaps of immunodeficient mice. grafts of a human cervical can ... | 1990 | 2172120 |
| sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation. | the sensitivity of digoxigenin and biotin labelled dna probes for the detection of human papillomavirus (hpv) by dot blotting and in situ hybridisation was compared in tissues from cervical, laryngeal, and anogenital neoplasia. probes were either labelled with digoxigenin by the random primer technique and detected with anti-digoxigenin antibody, or labelled with biotin by nick translation and detected with streptavidin, both methods having a common final visualisation procedure using alkaline p ... | 1990 | 2172319 |
| use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. | the polymerase chain reaction was adapted to the amplification of a herpes simplex virus (hsv) dna sequence, common to hsv types 1 and 2 (hsv-1, hsv-2). the amplified product was detectable by ethidium-bromide staining or southern hybridization of gels and by dot hybridization. the hsv polymerase chain reaction detected hsv dna in samples obtained from eight patients with genital lesions from which hsv-2 was isolated in tissue culture and from four patients with labial lesions from which hsv-1 w ... | 1990 | 2172392 |
| [studies on pathogenesis of cervical carcinoma based on the analysis of growth and differentiation mechanism of cervical epithelium]. | recently cervical cancer is defined as a sexually-transmitted disease, and human papillomavirus (hpv) has been focused as one of its etiologic agents. it is known that cervical cancer is extraordinarily rare in non-human mammals that have the estrous cycle. in contrast, cervical cancer is frequent in human beings which have lost the estrous cycle, and subsequently evolved a sexual behavior irrespective of the menstrual phase. therefore, upon the hypothesis that the estrous cycle is a period prot ... | 1990 | 2172418 |
| [molecular biologic study on the carcinogenesis of hpv in uterine cervical cancer and related lesions--analysis of hpv types 16, 18 e6/e7 gene mrna]. | recent molecular biologic studies have shown that some type of human papillomavirus (hpv) is closely associated with uterine cervical cancer. in order to investigate the relationship between hpv dna and cervical cancer, we studied the carcinogenesis of hpv in cervical cancer and related lesions by dot blot hybridization (d.b.h.), southern blot hybridization (s.b.h.), in situ hybridization (i.s.h.) and polymerase chain reaction (p.c.r.). we also followed some hpv dna, mrna positive or negative ca ... | 1990 | 2172419 |
| human papillomavirus type 8 contains cis-active positive and negative transcriptional control sequences. | human papillomavirus type 8 (hpv-8) is one aetiological agent of macular and flat wart-like lesions in patients with epidermodysplasia verruciformis and appears to be closely linked to skin carcinogenesis. a 1.2 kb region of the genome, which was previously shown to contain a viral e2-dependent enhancer, was progressively shortened from both ends with bal 31. the resulting fragments were tested for their ability to stimulate chloramphenicol acetyltransferase (cat) expression from the simian viru ... | 1990 | 2172459 |
| effect of perilesional injection of alpha-interferon on cervical intraepithelial neoplasia and associated human papillomavirus infection. | in this controlled prospective study, 14 patients with cervical intraepithelial neoplasia (cin) were observed for one month to exclude spontaneous regression, and then seven patients were treated twice-weekly with perilesional injections of alpha-interferon. both groups were monitored colposcopically, and advised to use the contraceptive sheath. cervical smears and biopsies for histology and viral studies were taken before and after the study. there was an improvement of six out of seven of the ... | 1990 | 2172535 |
| comparison of southern transfer hybridization and dot filter hybridization for detection of cervical human papillomavirus infection with types 6, 11, 16, 18, 31, 33, and 35. | a commercial dot filter hybridization kit (virapap kit) was compared with southern transfer hybridization for the detection of seven types of human papillomavirus (hpv) in cervical specimens from 450 consecutive females attending a sexually transmitted diseases clinic. in comparison with southern transfer hybridization, performed with the same probes used in the dot filter kit, the sensitivity, specificity, and positive and negative predictive values of dot filter hybridization were 90%, 94%, 74 ... | 1990 | 2173397 |
| survey of histologic specimens of human cancer for human papillomavirus types 6/11/16/18 by filter in situ hybridization. | histologic specimens (317) of genital and nongenital cancers and normal tissue were analyzed for the presence of the dna of human papillomavirus (hpv) 6, 11, 16, and 18 by filter in situ hybridization performed on paraffin-embedded, formalin-fixed tissue (histofish). hpv dna was found in 73 of 172 (42%) anogenital lesions and 17 of 116 (15%) nonanogenital carcinomas. no hpv dna was found in normal mouse skin (five samples), human autopsy liver (two samples), or kidney (eight samples), or in carc ... | 1990 | 2173398 |
| time trends in the prevalence of human papillomavirus infections in archival papanicolaou smears: analysis by cytology, dna hybridization, and polymerase chain reaction. | a retrospective study was undertaken to determine the prevalence of human papillomavirus (hpv) infections in routine papanicolaou (pap) smears collected by general practitioners from western australian women in each of the years 1972, 1982, and 1987. hpv infection was detected by cytology, dot-blot hybridization, or polymerase chain reaction (pcr). it was found that the prevalence of hpv infection remained unchanged over the 15 year study period, was independent of age, and was associated with n ... | 1990 | 2173733 |
| [asymptomatic and subclinical human papillomavirus infections of the genital organs and anus in males]. | | 1990 | 2173853 |
| cytologic correlates of cervical papillomavirus infection. | a fundamental question in papanicolaou smear screening is the specificity of cytologic criteria for the recognition of genital human papillomavirus (hpv) infection. to address this problem, we conducted a two-phase study of routinely screened women to determine the efficiency with which cytologic findings identified the presence of hpv dna, focusing on the criteria for identifying smears as "atypical." in phase 1, 25 of 290 (8.6%) smears were designated atypical, but only 3 (12%) of the samples ... | 1990 | 2174025 |
| genetic strategies of tumor suppression. | the evaluation of the cancer cell is a complex multigene process. tumor suppressor genes that are lost or inactivated, as well as genes that are overexpressed, play key roles in tumor progression. the identification of overexpressed genes has been expedited by the presence of transforming genes in some animal retroviruses. however, tumor suppressor genes have been difficult to identify and isolate because of their loss or inactivation during tumorigenesis. by a variety of methods, summarized in ... | 1990 | 2174661 |
| synthetic peptides derived from e7 region of human papillomavirus type 16 used as antigens in elisa. | nine overlapping peptides (20 amino acid) covering the entire sequence of early antigen e7 of human papillomavirus type 16 (hpv-16) were synthesized and tested as antigens with human sera in elisa. five of these peptides (no. 1 to 5 counting from the n terminus of the e7 protein) reacted with a pool of sera from hpv-16-infected individuals (as determined by molecular hybridization with their biopsy specimens); one (no. 5) was also reactive with pools of hpv-18- and hpv-6- or 11-infected individu ... | 1990 | 2174961 |
| human papillomavirus infection in sexually active adolescent females: prevalence and risk factors. | we examined the prevalence of human papillomavirus (hpv) infection, and associated risk factors for infection with hpv types 6, 11, 16, 18, 31, 33, and 35, in 661 sexually active adolescent females attending family planning clinics. fifteen percent were positive for hpv dna by rna-dna dot-blot hybridization. more than 60% of the hpv-positive subjects harbored at least one of the following cancer-related hpv types: 16, 18, 31, 33, or 35. those with hpv had a mean range of four to 10 lifetime sexu ... | 1990 | 2175024 |
| vestibular papillae of the vulva. lack of evidence for human papillomavirus etiology. | vestibular papillae of the vulva are usually considered as anatomical variants of the vestibular mucosa. clinically, however, they are quite often interpreted as condylomata acuminata and recent studies have suggested that they could be related to human papillomavirus infection. this prompted us to search for human papillomavirus dna using the southern blot hybridization technique, by analyzing biopsy specimens taken from 29 patients who presented with papillae of the vestibular mucosa. human pa ... | 1990 | 2175164 |
| human papillomavirus associated with keratoacanthomas in australian patients. | | 1990 | 2175166 |
| [detection of human papillomavirus dna in the normal genitalia of men]. | the study of human papillomavirus (hpv) in the genitalia of all normal males is difficult, therefore cases of chronic prostatitis, a common disease treated in urological clinics, were chosen to identify hpv dna by using vira type (life technologies inc.). smears of glans and sulcus coronalius of 177 subjects, showed a hpv positive rate of 3.4%, while 86 cases of those cases were negative for hpv in urethral smears. the lack of clinical findings suggests that hpv is an asymptomatic infection. in ... | 1990 | 2175330 |
| cervical intraepithelial neoplasia iii in an adolescent with bowenoid papulosis. | bowenoid papulosis (bp) is a cutaneous condition of the external genitalia seen primarily in young adults. evidence supports an etiologic role of human papillomavirus (hpv), particularly type 16. hpv-16 is also associated with an increased risk for cervical intraepithelial neoplasia (cin) and invasive carcinoma. a 17-year-old female, referred to the adolescent dysplasia clinic with a diagnosis of condyloma acuminatum, was presented. she had multiple smooth, shiny, brown 3- to 4-mm papules on the ... | 1990 | 2175743 |
| fast multiplex polymerase chain reaction on boiled clinical samples for rapid viral diagnosis. | an assessment of optimal conditions for rapid simultaneous amplification of multiple human papillomavirus (hpv) sequences has been made using thermus aquaticus dna polymerase. all variables of practical value were studied by amplifying known target-sequences from ten-fold dilutions of well characterized cell lines. in our hands, fast multiplex pcr (fm-pcr), the technique of running multiple pcr reactions simultaneously with minimum incubation time at each temperature, was highly sensitive (ampli ... | 1990 | 2175751 |
| human papillomavirus in oesophageal squamous cell carcinoma. | thirty seven cases of oesophageal squamous cell carcinoma were studied by applying dna slot blot analysis and in situ hybridisation using type specific probes for hpv 6, 11, 16 and 18. cases of condyloma accuminata, cervical carcinoma, and laryngeal papilloma were used as controls. blocks including areas of invasive carcinoma, intraepithelial neoplasia, and normal epithelium were studied in each case. no hpv genome was detectable in any of the oesophageal cases. it is concluded that these types ... | 1990 | 2175754 |
| cell differentiation-related gene expression of human papillomavirus 33. | the gene expression of human papillomavirus (hpv) 33, which can be detected both in benign and malignant genital tumors, was analyzed in a cervical condyloma acuminatum by in situ hybridization using open reading frame-specific rna probes. viral mrna concentrations increased with the degree of differentiation of the keratinocytes. the probes for reading frames e4 and e5 generated the most intense signals. the patterns of the specific viral mrnas were very similar to those in condylomas induced b ... | 1990 | 2175837 |
| outcome in mild and moderate cervical dysplasias related to the presence of specific human papillomavirus types. | in situ hybridization with biotinylated dna viral probes (ish-b) for human papillomavirus (hpv) types 6/11, 16, 18, 31, and 33 was used to study the outcome in 32 cases of mild and 21 cases of moderate cervical dysplasia with koilocytotic change that were followed for an average of 27 mo. the rates of regression, persistence, and progression for cervical intraepithelial neoplasia (cin) i and cin ii were 50%, 41%, and 9%, and 43%, 48%, and 9%, respectively. while progression of hpv 16 cin i and i ... | 1990 | 2175897 |
| [detection of human papillomavirus (hvp) 16/18 infections of the vaginal uterine cervix in females before and following conisation]. | 280 women have been tested by fish to detect an hpv 16/18-infection of the portio uteri. in a group of 133 women treated by conisation (all with a history of abnormal smear and colposcopy) 67 (50%) were positive for hpv 16/18 dna before the operation. follow up examinations of 20 cases with positive hpv 16/18 test before conisation, showed 6 and 12 months after operation only in 3 cases a persistence of hpv 16/18 infection. only 5 (6.4%) of 78 women with a history of conisation and diagnosis cin ... | 1990 | 2176023 |
| prevalence of human papillomavirus types 16 and 18 in cervical carcinomas: a study by dot and southern blot hybridization and the polymerase chain reaction. | histologically classified biopsies from 83 women with invasive cervical carcinoma were analyzed by dot blot hybridization for human papillomavirus (hpv) types 16 and 18 infection. sixty of the 83 (72.3%) were found to contain hpv dna, of which 43 (51.8%) contained hpv 16 dna, 12 (14.5%) contained hpv 18 dna and 5 (6.0%) contained both hpv 16 and 18 dnas. southern blot analysis on 65 specimens gave similar results. of 23 specimens negative by dot blot, 21 were tested by the polymerase chain react ... | 1990 | 2176202 |
| use of cervical cytobrush samples for dot-blot detection and southern blot typing of human papillomaviruses using subgenomic probes. | the use of cytobrush samples for the detection and typing of cervical human papillomavirus (hpv) infections was analyzed. the average yield of squamous cells from cytobrush samples was on the order of 10(6) cells when the sample was collected in a lytic collection buffer, which was approximately double the content of (1) samples collected in ethanol and (2) an average biopsy specimen. the material obtained could be used for sensitive detection and typing of hpv infections using, respectively, a ... | 1990 | 2176501 |
| colposcopy, punch biopsy, in situ dna hybridization, and the polymerase chain reaction in searching for genital human papillomavirus (hpv) infections in women with normal pap smears. | to assess the prevalence of hpv infection in the genital tracts of women with normal pap smears, a random series of 109 women was reexamined using colposcopy, a further pap smear, and punch biopsies taken from the cervix (in 33 cases), vagina (212 cases), and anus (20 cases). the biopsy material was examined using routine histological investigations, in situ hybridization (ish) with a 35s-labelled dna probe cocktail (hpv 6, 11, 16, 18), and the polymerase chain reaction (pcr) to detect hpv dna. ... | 1990 | 2176670 |
| the detection of human papillomavirus (hpv) in a case of minimal deviation adenocarcinoma of the uterine cervix (adenoma malignum) using in situ hybridization. | hpv-16 and hpv-18 dna sequences were detected in a case of minimal deviation adenocarcinoma (mda), a rare pathological type of uterine cervical cancer. this result suggests hpv to be associated with mda as well as with other pathological types of cervical cancer. | 1990 | 2178197 |
| detection and typing of human papillomavirus infection of uterine cervix in young women by non-isotopic subgenomic probes on southern blot--a report of studies in sweden. | human papillomavirus (hpv) was detected by non-isotopic subgenomic probes on southern blot hybridization (oncor inc., gaithenberg, md). the 108 samples which were obtained from patients below the age of 30 (mean age 19.0 +/- 2.8 yrs). all samples were collected from the ectocervical mucosa by cytobrush (medscand, malmö, sweden) as previously described. hpv-dna was detected in 16 cases (15%). in 36 cases (33%) the patients presented clinical findings of condyloma or cervical intra-epithelial neop ... | 1990 | 2178204 |
| ligneous conjunctivitis: an ophthalmic disease with potentially fatal tracheobronchial obstruction. laryngeal and tracheobronchial features. | ligneous conjunctivitis is a rare disease of unknown cause characterized by pseudomembranous, fibrous, woody, plaquelike deposits on the conjunctiva. the disease appears to be hereditary and/or familial. deposits similar to those found in the eye occur in the larynx, tracheobronchial tree, nose and nasopharynx, and vagina. when these lesions occur in the larynx and tracheobronchial tree, voice change and potentially life-threatening obstruction and pulmonary disease may occur. this paper reports ... | 1990 | 2195957 |
| ki-ras oncogene mutations in non-hpv-associated anal carcinoma. | human papillomavirus 16 (hpv 16) dna is found in a high proportion of anal squamous cell carcinomas in whose genesis it is thought to play an important role. in addition, it can be shown to cooperate in vitro with activated ras oncogenes in cellular transformation. we have therefore screened a series of such tumours for activating mutations of the ras oncogene family using dna amplified in vitro by the polymerase chain reaction (pcr) and a series of synthetic oligonucleotide probes. mutations we ... | 1990 | 2199641 |
| risk of cervical cancer among wives of men with carcinoma of the penis. | the relationship between carcinomas of the penis and cervix uteri was analyzed in married couples. the final series comprised 239 patients with squamous cell carcinoma of the penis and their 224 wives. two wives were found to have squamous cell carcinoma of the cervix uteri. the expected number of cervical cancers within the group of wives was 1.88, the relative risk thus being 1.05 (95% confidence interval 0.13-3.8). the incidence of condylomas has increased during the recent decades, while tha ... | 1990 | 2206567 |
| viral oncogenesis and the immune system. | oncogenic transformation of normal cells and the establishment of transformed cells to form malignant tumors is a complex, multistep process influenced by viruses in multiple ways. the relationship between viruses and the immune system manifests itself, in part, through various roles of viruses in transformation of host cells, including cells of the immune system. a large number of viruses participate in oncogenic transformation of cells in many animal species. candidates for oncogenic transform ... | 1990 | 2224909 |
| human immunodeficiency virus infection and cervical neoplasia. | to determine the relationship between human immunodeficiency virus (hiv) infection and cervical neoplasia, the characteristics of invasive and preinvasive cervical disease in 114 patients of known hiv status were assessed. seven of thirty-seven patients (19%) under age 50 with invasive cervical carcinoma were hiv-positive, including a 16-year-old with stage iiib disease. hiv-positive patients had more advanced invasive cancer than hiv-negative patients. disease persisted or recurred in all hiv-p ... | 1990 | 2227552 |
| clinical significance of hyperkeratosis and parakeratosis in otherwise negative papanicolaou smears. | to ascertain the clinical significance of hyperkeratosis and parakeratosis in otherwise negative papanicolaou smears, we retrospectively reviewed 96 patients with these findings seen during a 32-month period. evaluation included repeating the smear and performing colposcopy in all patients. colposcopically directed biopsy and endocervical curettage were performed when appropriate. only one patient had human papillomavirus (hpv) infection. the isolated finding of hyperkeratosis or parakeratosis w ... | 1990 | 2227586 |
| assessment and management of cervical intraepithelial neoplasia. | although cervical intraepithelial neoplasia (cin) once was thought to be a clinical problem primarily for older women, data reveal an alarming rise of this condition among younger women. the gains made in decreasing the incidence of cervical cancer have been tempered by the steadily increasing incidence of its precursor state, cin. because of increasing evidence of the association of the human papillomavirus (hpv) with cervical cancer and cin, this virus is of particular concern. colposcopy is a ... | 1990 | 2234556 |
| aggressive evaluation for atypical squamous cells in papanicolaou smears. | a retrospective study was done on women who had atypical papanicolaou smears and were referred for immediate colposcopy. the smears were obtained during january 1985 to march 1989 at edwards air force base, california. excluded from the evaluation were abnormal papanicolaou smears with hyperkeratosis, parakeratosis and koilocytotic atypia suggestive of human papillomavirus (hpv) infection. the evaluation included colposcopy, colposcopically directed biopsies, endocervical curettage and repeat pa ... | 1990 | 2246766 |
| estrogen receptor localization in normal and neoplastic epithelium of the uterine cervix. | to investigate the estrogen receptor (er) status of cells during carcinogenesis of the uterine cervix, the immunohistochemical reactivity for a monoclonal anti-er antibody (h 222) was studied in 26 normal cervical specimens, 21 cases of cervical intraepithelial neoplasia (cin), and 21 cases of invasive cervical carcinoma. in addition, the presence of human papillomavirus (hpv) dna (types 6/11, 16/18, or 31/33/35) was analyzed by in situ hybridization. in the normal cervix, basal cells of the squ ... | 1990 | 2249202 |
| evidence against diathermy as a beneficial treatment for human papilloma virus infection of the cervix. | | 1990 | 2256869 |
| a modified terminology for cervical intraepithelial neoplasia. | recent data are consistent with the concept that human papillomavirus (hpv) is etiologically important in the causation of cervical squamous cell cancer. there appear to be certain important events in the process of hpv infection and neoplasia. it is suggested that the terminology of the hpv-related precursor lesions be modified and that two terms, rather than three, would best satisfy the requirements of both science and clinical care. the "early" lesions should be referred to as "low-grade cer ... | 1990 | 2296409 |
| uterine cervical punch biopsy specimens can be analyzed by 1h mrs. | biopsy specimens of the uterine cervix, including colposcopically directed punch biopsy specimens of females with atypical papanicolaou smear tests, are suitable for analysis by magnetic resonance (mr) spectroscopy. a narrow lined lipid mr spectrum, characteristic of malignant tissue, is obtained from a 6-mm3 biopsy specimen of histologically confirmed squamous carcinoma of the cervix. in contrast, specimens containing inflammatory cells generate a broad component only centered at 1.3 ppm with a ... | 1990 | 2314221 |
| genetic screening with exfoliated cervicovaginal cells collected by a noninvasive lavage technique performed during routine gynecologic cancer screening. | we have investigated and established the feasibility of the use of exfoliated cervicovaginal cells as an abundant source of genetic material. we routinely use this material for the detection by molecular hybridization of human papillomavirus infection of the cervix. we have analyzed deoxyribonucleic acid isolated from cervicovaginal cells by southern blot hybridization to detect a common restriction fragment length polymorphism on chromosome 13. we suggest that in the near future most, if not al ... | 1990 | 2316565 |
| colposcopy of patients with cytologic inflammatory epithelial changes. | of 3,308 cervical cytologies performed at a university health service between september 1986 and september 1987, 371 were reported as showing inflammatory epithelial changes (iecs). colposcopy was offered to all patients with this diagnosis and was actually performed on 200 (54%). of these women, 44 had an atypical transformation zone; of the 33 who were biopsied, 23 had histologic diagnoses ranging from human papillomavirus (hpv) infection (4 cases) to grade iii cervical intraepithelial neoplas ... | 1990 | 2321444 |
| detection of human papillomavirus dna in laryngeal squamous cell carcinomas by polymerase chain reaction. | the presence of human papillomavirus genomes-16 and -6b in metastatic cervical lymph nodes was examined in 34 cases of laryngeal carcinomas by means of polymerase chain reaction, which had been fixed in formalin and embedded in paraffin. human papillomavirus dnas extracted from paraffin-embedded tumor tissues were used for polymerase chain reaction with amplification of the e6 region of human papillomavirus genome-16 and the e1 region of human papillomavirus genome-6b. human papillomavirus genom ... | 1990 | 2348745 |
| cancer incidence correlations: genital, urinary and some tobacco-related cancers. | bivariate correlations between sex-specific age-adjusted incidence rates of 10 genital and urinary tract cancer sites and lung cancer were calculated. the data base used was that published in the 5th edition of the series cancer incidence in five continents. the purpose of the analyses was to test the consistency of the data with the hypothesis of a common risk factor for genital cancers and other cancers of the urinary tract and for lung and genital cancers. the results indicate that the incide ... | 1990 | 2384268 |
| flat condylomata of the penis presenting as patchy balanoposthitis. | fifty patients presenting with either primary or recurrent patchy balanoposthitis which became aceto-white after the application of 5% acetic acid underwent biopsy. microbiological screening, including grocott staining of the biopsy material for fungal hyphae was performed. forty nine biopsies showed marked koilocytosis suggestive of human papilloma virus (hpv) infection, and of these, only three showed fungal hyphae. this study suggests that hpv may be associated with some cases of patchy balan ... | 1990 | 2391111 |
| in situ hybridization analysis of hpv 16 dna sequences in early cervical neoplasia. | the authors examined 18 cervical intraepithelial neoplasms (cin) for the presence of human papillomavirus (hpv) dna sequences by southern blot hybridization and dna-dna in situ hybridizations for hpv dna sequences and compared the epithelial distribution of hpv 16 dna sequences with hpv 6/11 sequences in selected condylomas. fifteen of the 18 cin lesions contained hpv 16 dna as determined by southern blot hybridization. with the use of biotinylated hpv 16 dna probes, 10 of the 18 were positive b ... | 1986 | 2421580 |
| association of human papillomavirus with squamous carcinoma of the uterine cervix. | the frequent association of human papillomavirus infection with preinvasive and invasive cancers of the cervix has led to speculation that the viruses may be involved in the neoplastic process. we have carried out some epidemiological studies of the prevalence of human papillomavirus infection in the female genital tract to evaluate more fully the oncogenic potential of these viruses in the cervix. a non-invasive method of detecting the virus has been developed for this purpose using dna hybridi ... | 1986 | 2424677 |
| epidermodysplasia verruciformis. skin carcinoma containing human papillomavirus type 5 dna sequences and primary hepatocellular carcinoma associated with chronic hepatitis b virus infection in a patient. | in a case of epidermodysplasia verruciformis with impaired cell-mediated immunity and multiple skin cancers human papillomavirus type 5 (hpv5) dna sequences were demonstrated in a cutaneous squamous cell carcinoma. hpv5 and hpv8 were detected in the benign disseminated skin lesions together with three newly characterized hpvs: hpv17, hpv19 and hpv24. a chronic infection with hepatitis b virus resulting in macronodular cirrhosis associated with a primary hepatocellular carcinoma was also acquired ... | 1986 | 2426900 |
| the l2 open reading frame of human papillomavirus type 1a encodes a minor structural protein carrying type-specific antigens. | the proteins encoded by the open reading frames of papillomavirus genomes and the minor polypeptides detected in purified virions are still poorly defined. we show here by its expression in escherichia coli that the open reading frame l2 of human papillomavirus type 1a codes for a minor structural protein of mr 76,000. antisera raised against a truncated l2-beta-galactosidase fusion protein in which the conserved n-terminal region of l2 is missing are type specific for human papillomavirus type ... | 1986 | 2430112 |
| transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 dna. | human keratinocytes and fibroblasts isolated from foreskin were transformed by transfection with recombinant human papillomavirus type 16 (hpv16) dna. the transformed cells exhibited an extended (fibroblasts) or indefinite (keratinocytes) life-span compared with that of normal controls. in addition, hs27, a human fibroblast cell line previously transfected with origin-defective simian virus 40, was successfully transfected. hpv16 sequences were stably maintained in the cells, and extensive ampli ... | 1987 | 2434663 |
| laryngeal papilloma cells in culture have an altered cytoskeleton. | we have investigated the effect of human papillomavirus (hpv) transformation on cellular cytoarchitecture. cells from laryngeal papillomas and normal epithelium were cultured in vitro. cytoskeletal components of both types of cells were visualized by immunofluorescence, to determine whether there were any differences in the structure or distribution of the cytoskeleton. there was no significant change in microtubules. two major components of the cytoskeleton, the intermediate filaments and the m ... | 1987 | 2437763 |
| structure, dnasei hypersensitivity and expression of integrated papilloma virus in the genome of hela cells. | three integrated copies of human papilloma virus-18 (hpv-18) have been identified in hela dna as hindiii bands. hpv-18 has no hindiii restriction site in its genome. the three segments: a, 8.4 kb; b, 7.9 kb and c, 5.8 kb, have an incomplete viral genome. all of them have most of the 1.1-kb bamh1 non-coding fragment of hpv-18, which seems to contain the viral origin of replication and regulatory elements. two of the segments (a and b) have a common 5'-end break-point in the viral genome within th ... | 1987 | 2439332 |
| squamous carcinoma of the lung in a nonirradiated, nonsmoking patient with juvenile laryngotracheal papillomatosis. | juvenile laryngotracheal papillomatosis (jltp)--a disease characterized by the growth of multiple, recurrent, koilocytolic papillomas of the upper respiratory tree--occurs in 1,500 to 2,000 infants and children in the united states every year. in rare instances, the papillomas, which are thought to be caused by a human papillomavirus (hpv), may extend into the distal bronchi and lungs. they are often excised surgically or by laser resection, but may regress spontaneously. patients who receive ra ... | 1987 | 2441616 |
| analysis of the biological role of human papilloma virus (hpv)-encoded transcripts in cervical carcinoma cells by antisense rna. | | 1987 | 2450820 |
| cervical lesions in patients with malignant melanoma. | koilocytes were found in the cervical epithelium in association with cervical intra-epithelial neoplasia in 8 out of 9 cases of malignant melanoma. this suggests that the cervical lesion in them was of viral origin. in 62 women with malignant melanoma but without cervical atypia there was also an excess of koilocytosis compared with controls with neither lesion. these findings point to the possibility that human papilloma virus infection may also be involved in the development of malignant melan ... | 1988 | 2453994 |
| condyloma acuminatum of the bladder in two autopsy cases. | condyloma acuminatum is a very rare lesion occurring in the bladder. we report two cases of condyloma acuminatum of the bladder found at autopsy, one in a 96-year-old woman and the other in an 80-year-old man. both had papillary or polypoid tumorous lesions in the trigone and neck of the bladder. the lesions were composed of nodular or papillary growths of squamous epithelium. the epithelium exhibited koilocytosis and the nuclei showed immunohistochemical staining for human papillomavirus antige ... | 1988 | 2455958 |
| characterization of normal human exocervical epithelial cells immortalized in vitro by papillomavirus types 16 and 18 dna. | an in vitro system for studying the interaction between human papillomavirus (hpv) 16 and 18 recombinant dna and normal human exocervical epithelial cells is described. eight hpv-immortalized human exocervical epithelial cell lines were established; all the lines contained either integrated hpv16 or 18 sequences and expressed hpv mrnas. thus, integration and expression appear to be required for immortalization. immortalized cells (greater than 200 population doublings to date) divided rapidly (d ... | 1988 | 2456144 |
| analysis of hpv-1 e4 gene expression using epitope-defined antibodies. | six monoclonal antibodies (mabs) have been raised against the e4 proteins of hpv-1. five of these were found to recognize denaturation-resistant epitopes as determined by western blotting--and their binding sites were identified by determining their reactivity against a panel of bacterial e4--beta-galactosidase fusion proteins which contained progressive deletions at the c-terminal end of the e4 region. the five mabs were found to bind to four distinct sites. by using these epitope-defined mabs, ... | 1988 | 2456213 |
| selective suppression of human papillomavirus transcription in non-tumorigenic cells by 5-azacytidine. | the transcription of human papillomavirus type 18 (hpv 18) is selectively suppressed in non-tumorigenic hela x fibroblast or hela x keratinocyte cell hybrids by 5-azacytidine. in contrast, viral gene expression is not influenced by 5-azacytidine in both tumorigenic hybrid segregants and in the parental hela cells. the suppression mechanism seems to operate at the level of initiation of transcription since nuclear run-on experiments show the absence of elongated nascent viral rna, whereas the tra ... | 1988 | 2457495 |
| immortalization of human foreskin keratinocytes by various human papillomavirus dnas corresponds to their association with cervical carcinoma. | normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (hpv) dnas (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. only transcripts from the intact e6 and e7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization. recombinant hpv ... | 1989 | 2462058 |
| phosphorylation of the human papillomavirus type 1 e4 proteins in vivo and in vitro. | following incubation of hpv 1-induced warts in the presence of [32p] phosphate several of the e4-encoded proteins were found to be radiolabeled. two-dimensional isoelectric focusing sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the 17k e4 polypeptides had incorporated [32p]phosphate whereas those of 16k were unlabeled. purified e4 gene products were separated by ion exchange chromatography into a large number of different species, which were of similar size but of diff ... | 1989 | 2470193 |
| a synthetic peptide defines a serologic iga response to a human papillomavirus-encoded nuclear antigen expressed in virus-carrying cervical neoplasia. | the growing awareness of the role of human papillomavirus (hpv) in cervical carcinoma has triggered a search for uncomplicated detection methods. to define a serologic response to hpv, we synthesized peptides based on sequences deduced from the genome of hpv type 16, the most common malignancy-associated type of hpv. one of these peptides reacted with iga antibodies present in sera from 24 of 33 patients with cervical intraepithelial neoplasia or cervical carcinoma, whereas this peptide reacted ... | 1989 | 2471193 |
| possible cofactors in the etiology of cervical intraepithelial neoplasia. an immunopathologic study. | previous work in our department demonstrated a reduction in the numbers of langerhans' cells in cervical epithelium showing histologic changes of human papillomavirus (hpv) infection and cervical intraepithelial neoplasia (cin). in conjunction with a localized reversal of the t4:t8 ratio of t-lymphocytes, that finding provides evidence of the association of epithelial immunosuppression with both hpv infection and cin. to investigate whether that phenomenon occurs primarily because of hpv alone o ... | 1989 | 2478707 |
| a cell mutant that exhibits temperature-dependent sensitivity to transformation by various oncogenes. | we previously isolated a fisher rat fibroblast mutant, b812, that has the unique property of temperature-dependent transformation by various oncogenic retroviruses. at the permissive temperature (35 degrees c), this mutant was sensitive to oncogenic transformation and formed foci on a dish at the same frequency as did the parental fibroblast cell line. when kirsten murine sarcoma virus (ki-msv) was applied to the cells, the frequency of focus formation decreased more than 25-fold at the nonpermi ... | 1989 | 2479832 |
| transformation of human cells by oncogenic viruses supports permissiveness for parvovirus h-1 propagation. | parvovirus h-1 has been shown to suppress spontaneous and chemically or virally induced tumorigenesis in hamsters. in human cell culture systems propagation of h-1 is restricted to transformed cells, which are killed by h-1 infection, in contrast to normal diploid cells, which are nonpermissive for h-1. by analyzing the permissiveness of a variety of human cells for h-1, it was determined that the majority of tested transformed or immortalized cells which were permissive for h-1 contained the dn ... | 1989 | 2495371 |
| the significance of atypical cervical cytology in an older population. | to ascertain the significance of squamous atypia encountered during routine papanicolaou smear screening in an older population, we reviewed 115 consecutive patients over age 50 seen during a 3-year period. evaluation included repeating the smear and performing colposcopy in all patients. colposcopically directed biopsy and endocervical curettage were performed when appropriate. sixty-seven patients (58.3%) had atrophy, 43 (37.4%) were normal, two (1.7%) had cervical intraepithelial neoplasia gr ... | 1989 | 2535763 |
| human papillomavirus 16 dna in cervical cancers and in lymph nodes of cervical cancer patients: a diagnostic marker for early metastases? | human papillomavirus (hpv) 16 is most prevalent in cervical cancers and also persists in metastases. we examined hpv16-dna-positive primary cancers and several lymph nodes from each of 14 patients to evaluate the use of hpv16 dna as a diagnostic marker for the detection of early node involvement. the hpv16 dna was exclusively integrated in 39% of the primary cancers, predominantly episomal in 36%, and integrated and extrachromosomal to a similar extent in 25%. thirteen of 16 involved lymph nodes ... | 1989 | 2536006 |
| presence of catenated human papillomavirus type 16 episomes in a cervical carcinoma cell line. | human papillomavirus (hpv) is frequently associated with cervical carcinoma and derived cell lines. in primary tissues of the carcinoma, the viral genome may be present in episomal or integrated configuration. in cell lines, however, only integrated hpv sequences have been reported. in this article, we describe the presence of episomal type 16 hpv (hpv16), demonstrated by electron microscopy and two-dimensional agarose gel electrophoresis, in a cervical carcinoma cell line, cc7t/vgh, established ... | 1989 | 2536104 |
| optimization of human papillomavirus genotype detection in cervical scrapes by a modified filter in situ hybridization test. | optimal conditions for the screening of cervical scrapes for human papillomavirus (hpv) were investigated by using filter in situ hybridization. since integrated and episomal hpv can be found, cell lines containing viral dna in an integrated form (hpv in caski) or in an episomal state (bk virus-induced hamster tumor cells) were used for optimization experiments. an increase in sensitivity was achieved by alkaline denaturation and neutralization before the specimens were spotted onto the membrane ... | 1989 | 2536384 |
| prognostic significance of the presence of human papillomavirus dna in patients with invasive carcinoma of the cervix. | cases of invasive carcinoma of the uterine cervix were analyzed to determine whether the presence or absence of human papillomavirus (hpv) dna in the neoplasms was a contributing factor to their outcome. the presence of hpv dna was evaluated using in situ hybridization on formalin-fixed, paraffin-embedded tissue sections. eighty-five patients with cervical carcinoma who had been surgically evaluated were included in the study. data from these patients was analyzed retrospectively to determine su ... | 1989 | 2536587 |
| study of multiple human papillomavirus-related lesions of the lower female genital tract by in situ hybridization. | twenty-six women with multiple human papillomavirus (hpv)-related lesions of the lower genital tract were investigated by immunohistochemistry for the internal genus-specific capsid antigen of hpv and by dna-dna in situ hybridization with 35s-radiolabeled probes for sequences of hpv-6/11, hpv-16, and hpv-18. the vulva was the most frequently affected site in all of these cases; the cervix was the second most frequently affected site. the lesions displayed the features of papillomavirus infection ... | 1989 | 2536630 |
| numerous nuclear proteins bind the long control region of human papillomavirus type 16: a subset of 6 of 23 dnase i-protected segments coincides with the location of the cell-type-specific enhancer. | the long control region of the human papillomavirus type 16 genome is 856 base pairs (bp) long. it contains a cell-type-specific enhancer, a glucocorticoid response element, and sequences mediating the response to the viral gene products of open reading frame e2; all three regulate the promoter p97. we mapped binding sites of trans-acting proteins relevant for the cell-type-specific enhancer and other cis-acting elements by dnase i footprint experiments with nuclear extracts from hela cells. thr ... | 1989 | 2536825 |
| oncogenic and nononcogenic human genital papillomaviruses generate the e7 mrna by different mechanisms. | a new promoter located within e6 was mapped in human papillomavirus type 6b (hpv6b)- and hpv11-containing benign genital condylomata (genital warts). the rna transcribed from this promoter represented the major rna species colinear with open reading frames e6 and e7 and can encode the e7 protein. no equivalent promoter was active in hpv16-containing cancers and cancer-derived cell lines. in those, the major transcripts contained one of two different introns within e6 and the rnas could encode tw ... | 1989 | 2536845 |
| the multicentric nature of disease related to human papillomavirus infection of the female lower genital tract. | two hundred fifty-one consecutive patients from our colposcopy clinic were evaluated to establish the extent to which human papillomavirus (hpv) infection is a disease of the entire female lower genital tract. colposcopic examinations and biopsies of the cervix and vulva were performed on all patients. two hundred two women had cervical disease, of whom 164 (81%) also had vulvar disease. the percentage was the same regardless of the severity of the cervical disease. one hundred ninety-four of th ... | 1989 | 2536908 |
| the polymerase chain reaction: a new epidemiological tool for investigating cervical human papillomavirus infection. | the polymerase chain reaction is an in vitro method for primer directed enzymatic amplification of specific target dna sequences. the technique was used to detect human papillomavirus types 11 and 16 simultaneously in cellular dna recovered from cervical smears in 38 women referred for colposcopy to evaluate cytological abnormality and 10 women with no history of cytological abnormality. the polymerase chain reaction was shown to be both specific and sensitive in detecting human papillomavirus d ... | 1989 | 2537123 |
| human papillomavirus (hpv) type 6 and 16 dna sequences in bronchial squamous cell carcinomas demonstrated by in situ dna hybridization. | a series of 131 routinely processed, paraffin-embedded biopsy specimens derived from the same number of patients with a bronchial squamous cell carcinoma were analyzed using in situ dna-hybridization technique with a probe cocktail containing 35s-labeled human papillomavirus (hpv) dna of types 6, 11, 16, 18, and 30. the 12 carcinomas shown to contain hpv dna by the probe cocktail were subjected to in situ hybridization with the specific hpv dna probes applied separately under high stringency con ... | 1989 | 2537916 |
| human papillomavirus typing of penile condyloma. | infection with human papillomavirus has been identified as a significant risk factor for the development of cervical neoplasia. knowledge of the characteristics of infecting human papillomavirus in male patients is lacking. we studied 37 men who presented with genital condyloma to identify the clinical appearance of the lesion, histological features and human papillomavirus type as determined by deoxyribonucleic acid molecular hybridization analysis. of the patients 32 had penile shaft condyloma ... | 1989 | 2538663 |
| vulvar intraepithelial neoplasia: a clinicopathological study of carcinoma in situ of the vulva. | thirty-two patients with carcinoma in situ of the vulva were analyzed with respect to age-specific incidence rates, associated human papilloma virus (koilocytosis and condyloma) changes, and multifocal, unifocal distribution of the lesions. the association of koilocytosis and condyloma changes in the neoplastic epithelium correlated with a younger mean age compared to those without human papilloma virus (hpv) changes (39 versus 67 years). also, patients with multifocal disease were found to have ... | 1989 | 2539316 |
| [biological significance of hpv sequences integrated into cancer cells]. | in cervical cancer with integrated human papillomavirus (hpv) type 16 or 18 dna, e6 and e7 regions of hpv 16 or 18 were always present, suggesting that these regions play an important role in development of cervical cancer. structure and biological activity of cdnas representing two types of hpv 18 transcripts in hela cells were analyzed. three open reading frames (e6, e6* and e7) were identified. these open reading frames were introduced into the expression vectors. among them, e7 alone provide ... | 1989 | 2539785 |
| correlation of the histologic appearance of intraepithelial neoplasia of the cervix with human papillomavirus types. emphasis on low grade lesions including so-called flat condyloma. | cervical condylomas and intraepithelial neoplasia (cin) were correlated with human papillomavirus (hpv) types and analyzed for the presence of abnormal mitotic figures. colposcopically directed cervical biopsies were divided in half and processed for routine microscopy and southern blot hybridization. of 83 specimens from 71 patients, 70 (84%) contained hpv-dna sequences. the hpv distribution was as follows: hpv 16 in 6/25 flat condylomas (fc), 2/8 cin i, 8/18 cin ii, 12/14 cin iii; hpv 18 in 1/ ... | 1989 | 2540101 |
| human papillomavirus. current concepts. | | 1989 | 2540721 |
| an atypical case of penile carcinoma in situ associated with human papillomavirus dna type 18. | we report an unusual case of penile carcinoma in situ in a heterosexual man. the histologic study showed a severe dysplasia, and the typing of human papillomavirus dna, performed at the pasteur institute, disclosed a type 18, which is usually associated with penile and cervical carcinomas. the identification of an oncogenic human papillomavirus type in the penile lesions, such as type 18 found in this case, should lead to a search for cervical carcinoma in the sexual partner. | 1989 | 2541182 |
| colposcopy of condylomatous men. | the male partners of women diagnosed with human papilloma virus (hpv) or dysplasia were examined colposcopically. anogenital infection was found in 82% of the men, and dysplasia in two of the partners of dysplastic women. men may not be merely asymptomatic carriers of hpv. | 1989 | 2541328 |
| dna of human papillomavirus type 16 in dysplastic and malignant lesions of the conjunctiva and cornea. | human papillomaviruses are receiving attention for their role in the pathogenesis of cancer, especially cancer of the anogenital tract. although strains of human papillomavirus are associated with benign lesions of the conjunctiva, their association with conjunctival dysplastic lesions and carcinomas has remained unclear. we examined a group of neoplastic lesions of the conjunctiva for the presence of dna sequences for human papillomavirus types 16 and 18, using in vitro gene amplification with ... | 1989 | 2541337 |
| detection of the human papillomavirus 6b e2 gene product in genital condyloma and laryngeal papilloma tissues. | we have previously reported that the e2 open reading frame of human papillomavirus type 6b (hpv6b) was expressed in bombyx mori cell line bm-n as a fusion protein with baculovirus polyhedrin. here we show that antibody against this e2-polyhedrin fusion protein recognized a putative gene product as a 49-kda protein in some genital condyloma and laryngeal papilloma tissues, which were associated with hpv6 and hpv11, by immunoblotting analysis. the antibody reacted also with antigens in the nucleus ... | 1989 | 2541557 |
| juvenile and adult laryngeal papillomata: classification by in-situ hybridization for human papillomavirus. | we report the application of an in-situ hybridization technique for the demonstration of human papillomavirus (hpv) employing a biotin-streptavidin-polyalkaline phosphatase complex to paraffin processed tissue from a series of patients with laryngeal papillomata. all cases of juvenile papillomata, whether solitary or multiple, proved positive for hpv types 6 and/or type 11. however, only two cases of adult solitary papillomata and five cases of adult multiple papillomata were positive for hpv ty ... | 1989 | 2541952 |
| increased detection rate of human papillomavirus in cervical scrapes by the polymerase chain reaction as compared to modified fish and southern-blot analysis. | cervical scrapes from 80 women with a positive cytology result were tested for the presence of human papillomavirus (hpv) using the polymerase chain reaction (pcr) and compared to the results obtained with the modified filter in situ hybridisation (fish) and the southern-blot techniques. the sensitivity of the modified fish and the southern-blot was similar, and hpv was detected in 46% of the patients. the sensitivity of the pcr appeared to be higher, and hpv was detected in 70% of the patients. ... | 1989 | 2542439 |
| cloning and partial dna sequencing of two new human papillomavirus types associated with condylomas and low-grade cervical neoplasia. | using low-stringency southern blot analysis and cloning in lambda bacteriophage, two new human papillomavirus types (hpv-43 and hpv-44) were identified and their dnas were cloned from vulvar tissues. the isolates were characterized by restriction endonuclease mapping and shown to be new hpv types on the basis of their minimal hybridization with all other known hpv types at high stringency. both hpvs are most closely related to types 6, 11, and 13. hpv-43 did not exhibit any cross-reactivity with ... | 1989 | 2542593 |