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bioaccumulation, oxidative stress and hsp70 expression in cyprinus carpio l. exposed to microcystin-lr under laboratory conditions.microcystin-lr (mc-lr) produced by cyanobacteria are potent specific hepatotoxins. so far the pathogenesis of environmental mc-lr toxicity to aquatic organisms has not been fully elucidated. in the present study the accumulation of mc-lr was investigated in various organs/tissues of cyprinus carpio l. (c. carpio) following exposure to mc-lr for 14 d at environmentally relevant concentrations (0.1 to 10 μg l(-1)). results showed that the presence of mc-lr enhanced toxin accumulation in all invest ...201222240489
determination of hypoxia and dietary copper mediated sub-lethal toxicity in carp, cyprinus carpio, at different levels of biological organisation.hypoxic events frequently occur in the aquatic environment in association with micro pollutants, including heavy metals. only a few studies are however available on the uptake and biological responses of heavy metals under hypoxic conditions. to elucidate the phenomenon, mirror carp cyprinus carpio l. (16.13-16.22 g) were exposed chronically to dietary copper (cu; 250 and 500 mg kg dry wt.(-1)) for 30 d under normoxic (8.25 mg o(2) l(-1)) and hypoxic (~3 mg o(2) l(-1)) conditions and adopting an ...201222239943
editorial focus: myoglobin-2, an adaptation for life at low oxygen pressure. focus on: "functional differentiation of myoglobin isoforms in the hypoxia-tolerant carp". 201222237594
effects of atrazine and chlorpyrifos on activity and transcription of glutathione s-transferase in common carp (cyprinus carpio l.).glutathione s-transferase isoenzymes (gsts) play a critical role in detoxification pathways. here we report the tissue distribution of four antioxidant gsts gene in common carp, and their expression profiles. we also investigated the gsts activity in different tissues after exposure to the agricultural chemicals atrazine (atr), chlorpyrifos (cpf), and their mixture. relative changes in the mrna abundance of the gst isoforms were examined by real time pcr in liver, brain, kidney and gill of commo ...201222236720
effects of microcystin-containing cyanobacterial extract on hematological and biochemical parameters of common carp (cyprinus carpio l.).the aim of the study was to assess the effects of a cyanobacterial extract containing microcystins (mcs) on selected hematological and biochemical parameters in common carp (cyprinus carpio l.), as well as to determine the accumulation of toxins in fish tissues. the fish were immersed for 5 days in water containing toxins at a final concentration of 12 μg/l of microcystin lr equivalent. microcystin lr residues were detected in fish liver, reaching 207, 238 and 260 ng/g f.w. of the tissues taken ...201222228075
molecular cloning and expression of two β-defensin and two mucin genes in common carp (cyprinus carpio l.) and their up-regulation after β-glucan feeding.in this study, we described the partial structure, mrna tissue distribution and regulation of two carp mucin and two β-defensin genes. this is the first description of these genes in fish. the genes might provide relevant tools to monitor feed-related improvements of fish health under aquaculture conditions. carp mucin 2 and mucin 5b genes show a high similarity to their mammalian and avian counterparts. the carp β-defensin 1 and β-defensin 2 genes cluster together well with their piscine family ...201222227003
risk-benefit evaluation of fish from chinese markets: nutrients and contaminants in 24 fish species from five big cities and related assessment for human health.the risks and benefits of fish from markets in chinese cities have not previously been fully evaluated. in the present study, 24 common fish species with more than 400 individual samples were collected from markets from five big chinese cities in 2007. the main nutrients and contaminants were measured and the risk-benefit was evaluated based on recommended nutrient intakes and risk level criteria set by relevant authorities. the comprehensive effects of nutrients and contaminants in marine oily ...201222225822
morphological, physiological and behavioural response patterns of carp gudgeon hypseleotris spp. to food deprivation: implications for assessing health.morphological (growth, fulton's condition factor), physiological (per cent dry mass, total lipid content) and behavioural (activity levels) response patterns of carp gudgeon hypseleotris spp. were examined in response to food deprivation during a 56 day experiment. considerable variability in the nature and magnitude of these response patterns was observed, suggesting that caution should be taken when interpreting changes in the health of small-bodied fishes based on individual response variable ...201222220900
phenotypic variation and associated predation risk of juvenile common carp cyprinus carpio.juvenile common carp cyprinus carpio were collected from 10 lakes with variable predator abundance over 4 months to evaluate if morphological defences increased with increasing predation risk. cyprinus carpio dorsal and pectoral spines were longer and body depth was deeper when predators were more abundant, with differences becoming more pronounced from july to october. to determine if morphological plasticity successfully reduced predation risk, prey selection of largemouth bass micropterus sal ...201222220889
evolutionary history of c-myc in teleosts and characterization of the duplicated c-myca genes in goldfish embryos.c-myc plays an important role during embryogenesis in mammals, but little is known about its function during embryonic development in teleosts. in addition, the evolutionary history of c-myc gene in teleosts remains unclear, and depending on the species, a variable number of gene duplicates exist in teleosts. to gain new insight into c-myc genes in teleosts, the present study was designed to clarify the evolutionary history of c-myc gene(s) in teleosts and to subsequently characterize dna methyl ...201222213278
effects of 2,4-dinitrotoluene exposure on enzyme activity, energy reserves and condition factors in common carp (cyprinus carpio).in this study relative condition factor (rcf) and hepatosomatic index (hsi) as well as the available energy reserves of common carp (cyprinus carpio) by 2,4-dnt semi-static bioassay were determined and linked to effects of enzymes in liver tissues. fish were exposed at sublethal concentrations of 2,4-dnt (0.13 μg/l, 0.1, 0.5, 1.0mg/l) for 7 and 15 d. based on the results, there was no significant change in all parameters measured in fish exposed to 2,4-dnt at environmental related concentration, ...201222209323
diversity of cd2 subfamily receptors in cyprinid fishes.cd2 family receptor (cd2f) is evolutionarily conserved and is widely expressed by various types of leukocytes. to elucidate the phylogenetic diversity of the cd2f, we characterized cd2f in teleosts using ginbuna crucian carp and zebrafish. the identified cd2f isoforms of the ginbuna carp (caaucd2f) exhibited high sequence similarity to the mammalian cd2 subsets cd48, cd244, and cd319, but it was difficult to classify them into their respective mammalian cd2f based on sequence similarity, the pre ...201224371564
[quantitative trait locus analysis of standard length, body depth and body thickness in mirror carp (cyprinus carpio l.)].based on a full-sib family, the genetic linkage map was constructed with 246 microsatellite and 306 snp markers, which was used to detect the qtls for standard length (sl), body depth (h), body thickness (bt), and the ratio of standard length and body depth (slh) in mirror carp by gridqtl software. the results indicated that a total of 14 related qtls distributed on the 7 linkage groups were obtained. seven qtls were related to standard length, of which the linkage groups of lg6, lg17, lg21, lg2 ...201122207383
distribution of mono-, di- and trisialo gangliosides in the brain of actinopterygian fishes.mono-, di- and trisialo gangliosides are major glycosphingolipids in the brain of higher vertebrates involved in lipid raft assembly. in contrast, the fish brain is abundant in polisialo-gangliosides, whose function is implicated in the modulation of repulsive and attractive intercellular interactions during embryonic development and a temperature adaptation process. the histological distribution of gangliosides is usually studied in rodent and mammalian brains, but to date it has not been descr ...201222206893
patterns of metal composition and biological condition and their association in male common carp across an environmental contaminant gradient in lake mead national recreation area, nevada and arizona, usa.there is a contaminant gradient in lake mead national recreation area (lmnra) that is partly driven by municipal and industrial runoff and wastewater inputs via las vegas wash (lvw). adult male common carp (cyprinus carpio; 10 fish/site) were collected from lvw, las vegas bay (receiving lvw flow), overton arm (oa, upstream reference), and willow beach (wb, downstream) in march 2008. discriminant function analysis was used to describe differences in metal concentrations and biological condition o ...201222206697
cytochrome p450 1c1 complementary dna cloning, sequence analysis and constitutive expression induced by benzo-a-pyrene in nile tilapia (oreochromis niloticus).cyp1c is the newest member of the cyp1 family of p450s; however, its physiological significance, inducers, and metabolic functions are unknown. in this study, a new complementary dna of the cyp1c subfamily encoding cyp1c1 was isolated from nile tilapia (oreochromis niloticus) liver after intracoelomic injection with benzo-a-pyrene (bap). the full-length cdna was 2223 base pair (bp) long and contained an open reading frame of 1581 bp encoding a protein of 526 amino acids and a stop codon. the seq ...201222204985
kinetics and thiol requirements of iodothyronine 5'-deiodination are tissue-specific in common carp (cyprinus carpio l.).iodothyronine deiodinases determine the biological activity of thyroid hormones. despite the homology of the catalytic sites of mammalian and teleostean deiodinases, in-vitro requirements for the putative thiol co-substrate dithiothreitol (dtt) vary considerably between vertebrate species. to further our insights in the interactions between the deiodinase protein and its substrates: thyroid hormone and dtt, we measured enzymatic iodothyronine 5'-deiodination, dio1 and dio2 mrna expression, and d ...201222198122
novel positive-sense, single-stranded rna (+ssrna) virus with di-cistronic genome from intestinal content of freshwater carp (cyprinus carpio).a novel positive-sense, single-stranded rna (+ssrna) virus (halastavi árva rna virus, halv; jn000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (cyprinus carpio) fished by line-fishing from fishpond "lőrinte halastó" located in veszprém county, hungary. the complete nucleotide (nt) sequence of the genomic rna is 9565 nt in length and contains two long--non-in-frame--open reading frames (orfs), which are separated by an interge ...201122195010
molecular cloning and mrna expression of the vitellogenin and nuclear receptor gene induced by 17β-estradiol in the mud carp, cirrhinus molitorella.vitellogenins (vtgs), the precursors of yolk proteins, are crucial for the embryonic development of teleosts, and have also been studied extensively as biomarkers for environmental estrogenic mimics. the cdna of a vtg was isolated from the liver of male mud carp (cirrhinus molitorella) following induction by 17β-estradiol (e(2)) using 3'- and 5'-race methods. it was 4,216 bp in length, and encoded a putative protein of 1,342 amino acids. this putative vtg contained complete portions of lipovitel ...201222193541
muscle ankyrin repeat proteins: their role in striated muscle function in health and disease.remodeling is a stringently controlled process that enables adequate response of muscle cells to constant physical stresses. in this process, different kinds of stimuli have to be sensed and converted into biochemical signals that ultimately lead to alterations of muscle phenotype. several multiprotein complexes located in the sarcomere and organized on the titin molecular spring have been identified as stress sensors and signal transducers. in this review, we focus on ankrd1/carp and ankrd2/arp ...201622185618
pro-inflammatory functions of carp cxcl8-like and cxcb chemokines.numerous cxc chemokines have been identified in fish, however, their role in inflammation is not well established. here, cxc chemokines of the cxcl8-like (cxca_l1 and cxcl8_l2) and cxcl9/10/11-like (cxcb) subset were investigated in carp. recombinant cxca_l1, cxcl8_l2 and cxcb all stimulated chemotaxis of macrophages and granulocytes in vitro. cxcb also attracted lymphocytes. distinct effects on phagocyte activation were observed: the cxcl8-like chemokines increase respiratory burst activity, bu ...201222182503
[the consumption of dishes and/or fishy snacks by school youth from miedzychodzko-sierakowskie lakes].the school youth inhabiting miedzychodzko-sierakowskie lakes consumed dishes and/or fishy snacks on the similar low level as their peers in other regions of country, mainly breaded fried fishes and partly or highly manufactured products i.e., herrings in sour cream as well as pastes from smoked fishes and canned fishes. relatively high ranks in rows of preference (liking) and frequency intake were found for carp, herring and cheap cultured panga from import. the presence of bones and specific od ...201122171525
gastrointestinal absorption, metabolic debromination, and hydroxylation of three commercial polybrominated diphenyl ether mixtures by common carp.the gastrointestinal absorption, metabolic debromination, and hydroxylation of three commercial brominated diphenyl ether (bde) mixtures were separately studied in juvenile common carp. the absorption rate of penta-bde was higher than that of octa- and deca-bde, likely because of the lower molecular volumes of its major congeners. however, no significantly positive relationships were found between the number of bromine atoms and the absorption rate, especially for congeners with a bromine atom n ...201222170638
functional differentiation of myoglobin isoforms in hypoxia-tolerant carp indicates tissue-specific protective roles.because of a recent whole genome duplication, the hypoxia-tolerant common carp and goldfish are the only vertebrates known to possess two myoglobin (mb) paralogs. one of these, mb1, occurs in oxidative muscle but also in several other tissues, including capillary endothelial cells, whereas the other, mb2, is a unique isoform specific to brain neurons. to help understand the functional roles of these diverged isoforms in the tolerance to severe hypoxia in the carp, we have compared their o(2) equ ...201222170621
the effects of click 500 sc (terbuthylazine) on common carp cyprinus carpio under (sub)chronic conditions.effects of the herbicide formulation click 500 sc (terbuthylazine 500 g/l) on common carp cyprinus carpio were assessed through biometric, biochemical, haematological and antioxidant indices, induction of xenobiotic metabolizing enzymes and histological examination of selected tissues.201122167222
effects of neurotoxin - anatoxin-a on common carp (cyprinus carpio l.) innate immune cells in vitro.the aim of this study was to determine if cyanoneurotoxin - anatoxin-a (antx-a) alters the essential functions of innate immune cells such as free radicals generation in phagocytic cells and phagocytosis.201122167212
[toxicity of niclosamide with plastic film mulching to fish].to observe the toxicity of niclosamide with plastic film mulching to fish.201122164373
[tissue specificity of antioxidant system functioning and lipid peroxidation in different age groups of amur carp].key features of tissue enzymes functioning in antioxidant system (aos) in sexually mature and immature individuals of amur carp were studied. the activity of antioxidant enzymes was highest in the myocardium and subjected to age-related changes. it was concluded that changes in the functioning of aos and intensity of lipid peroxidation processes are characterized by organ-tissue metabolic features and age peculiarities of metabolism that is most expressed in the myocardium.201522145412
growth hormone promotes hair cell regeneration in the zebrafish (danio rerio) inner ear following acoustic trauma.previous microarray analysis showed that growth hormone (gh) was significantly upregulated following acoustic trauma in the zebrafish (danio rerio) ear suggesting that gh may play an important role in the process of auditory hair cell regeneration. our objective was to examine the effects of exogenous and endogenous gh on zebrafish inner ear epithelia following acoustic trauma.201122140580
dramatic increase of nitrite levels in hearts of anoxia-exposed crucian carp supporting a role in cardioprotection.nitrite (no(2)(-)) functions as an important nitric oxide (no) donor under hypoxic conditions. both nitrite and no have been found to protect the mammalian heart and other tissues against ischemia (anoxia)-reoxygenation injury by interacting with mitochondrial electron transport complexes and limiting the generation of reactive oxygen species upon reoxygenation. the crucian carp naturally survives extended periods without oxygen in an active state, which has made it a model for studying how evol ...201222129619
unique physicochemical properties of perfluorinated compounds and their bioconcentration in common carp cyprinus carpio l.carp (cyprinus carpio l.) was exposed to perfluorinated compounds (pfcs)-perfluoroalkyl carboxylic acids (number of carbon atoms, c = 8, 11, 12, 14, 16, and 18) and perfluorooctane sulfonate (pfos)-in bioconcentration tests to compare the bioconcentration factors (bcfs) and physicochemical properties of each specific compound. despite having the same number of carbon atoms (c = 8), the bcfs of perfulorooctanoic acid (pfoa) and pfos differed by more than two orders of magnitude (pfoa bcf = < 5.1 ...201222127646
sensory and textural attributes and fatty acid profiles of fillets of extensively and intensively farmed eurasian perch (percafluviatilis l.).sensory attributes, texture and fatty acid profiles of fillets of eurasian perch (percafluviatilis l.) reared under two conditions were compared. perch were reared either in an extensive pond-based (ec) system in polyculture with carp, or intensively cultured (ic) in a recirculation system. attributes of raw and cooked fillets of marketable perch (120-150g) were compared. no significant differences were found between groups for odour, flavour, aftertaste, or consistency in subjective evaluation ...201125212336
the role of neuromedin u during inflammatory response in the common carp.in the current study, we cloned and characterized the neuromedin u (nmu) gene from the common carp cyprinus carpio l., and identified its participation in immune responses in the teleost. five isoforms of the prepronmu genes were generated by alternative splicing and isolated from carp. the longest form of the carp prepronmu1 (isoform 1) cdna was composed of 803 bp, and contained an 18 bp 5'-utr, a 212 bp 3'-utr and a 573 bp open reading frame, which translates into a peptide comprising 190 amin ...201222122861
[mapping and genetic effect analysis of quantitative trait loci related to body length in mirror carp (cyprinus carpio l.)].a group of f2 hybrids of mirror carp including 68 individuals was used to construct a linkage map using joinmap4.0 software. of 560 markers genotyped, 507 markers (143 ssr markers, 33 est-ssr markers, and 321 snp markers) were assigned to the map, which comprised of 62 linkage groups. quantitative traits loci (qtls) associated with body length were identified by interval mapping of the software mapqtl 5.0. a linkage group wide permutation test (1 000 replicates) determined the significance of th ...201122120081
the consumption of the carp meat and plasma lipids in secondary prevention in the heart ischemic disease patients.omega-3 fatty acids (fa) have been shown to be protective against cardiovascular diseases (cvd). the effect of the consumption of carp meat on cvd risk factors has not yet been examined in detail. we ascertained the influence of a diet enriched with carp meat with an elevated content of omega-3fa (200 g twice weekly for 4 weeks) in a group of subjects after cardiac revascularization surgery for ischemic heart disease with a follow-up spa treatment.201122101876
pyrosequencing of 16s rrna gene amplicons to study the microbiota in the gastrointestinal tract of carp (cyprinus carpio l.).the microbes in the gastrointestinal (gi) tract are of high importance for the health of the host. in this study, roche 454 pyrosequencing was applied to a pooled set of different 16s rrna gene amplicons obtained from gi content of common carp (cyprinus carpio) to make an inventory of the diversity of the microbiota in the gi tract. compared to other studies, our culture-independent investigation reveals an impressive diversity of the microbial flora of the carp gi tract. the major group of obta ...201122093413
extracellular ph dynamics of retinal horizontal cells examined using electrochemical and fluorometric methods.extracellular h(+) has been hypothesized to mediate feedback inhibition from horizontal cells onto vertebrate photoreceptors. according to this hypothesis, depolarization of horizontal cells should induce extracellular acidification adjacent to the cell membrane. experiments testing this hypothesis have produced conflicting results. studies examining carp and goldfish horizontal cells loaded with the ph-sensitive dye 5-hexadecanoylaminofluorescein (haf) reported an extracellular acidification on ...201222090459
in vitro and in vivo hepatoprotective and antioxidant effects of astragalus polysaccharides against carbon tetrachloride-induced hepatocyte damage in common carp (cyprinus carpio).the present study is aiming at evaluating the hepatoprotective and antioxidant effects of astragalus polysaccharide (aps) on the carbon tetrachloride (ccl(4))-induced hepatocyte and liver injury in common carp in vitro and in vivo. in vitro, aps (200, 400 and 800 μg/ml) was added to the carp primary hepatocytes before (pre-treatment), after (post-treatment) and both before and after (pre- and post-treatment) the incubation of the hepatocytes with ccl(4) at 8 mm in the culture medium. aps at conc ...201222089693
atr-x syndrome in two siblings with a novel mutation (c.6718c>t mutation in exon 31).atr-x syndrome is an x-linked mental retardation syndrome characterized by mental retardation, alpha thalassaemia and distinct facial features which include microcephaly, frontal hair upsweep, epicanthic folds, small triangular nose, midface hypoplasia and carp-shaped mouth. here we report two brothers with clinical features of atr-x syndrome, in whom a novel missense (c>t) mutation was identified in exon 31 of the atrx gene.201122089611
molecular characterization and expression analysis of two new c-reactive protein genes from common carp (cyprinus carpio).c-reactive protein (crp) plays an important role in the acute phase response. transcripts encoding two new crp-like molecules (cccrp1 and cccrp2) from european common carp have been characterized which has enabled seven crp-like genes to be identified in zebrafish. 79.3% (cccrp1) and 74.5% (cccrp2) identity to crp from east-asian common carp occurs and fish crp genes form a distinct clade. cccrp2 gene organization comprises four exons and three introns, in contrast to the two exons/one intron or ...201222079493
effects of the commercial formulation containing fipronil on the non-target organism cyprinus carpio: implications for rice-fish cultivation.the aim of this research was to evaluate possible toxic effects of commercial formulation containing fipronil on cyprinus carpio tissues under rice field conditions. antioxidant profile (sod, catalase, glutathione s-transferase), oxidative stress parameters (thiobarbituric acid-reactive substances, protein carbonyl), and growth were investigated in carp exposed to fipronil under rice field conditions for 7, 30, and 90 days. waterborne insecticide concentrations were measured and the detectable c ...201222078114
influences of incorporating detoxified jatropha curcas kernel meal in common carp (cyprinus carpio l.) diet on the expression of growth hormone- and insulin-like growth factor-1-encoding genes.jatropha curcas is a drought-resistant shrub or small tree widespread all over the tropics and subtropics. the use of j. curcas (l) kernel meal in fish feed is limited owing to the presence of toxic and antinutritional constituents. in this study, it was detoxified using heat treatment and organic solvent extraction method. the detoxification process was carried out for 60 min to obtain the detoxified meal. cyprinus carpio l. fingerlings (n = 180; avg. wt. 3.2 ± 0.07 g) were randomly distributed ...201322077318
khawia japonensis (cestoda: caryophyllidea): another invasive parasite of carp, cyprinus carpio l., imported to europe. 201122074021
organochlorine pesticides and polychlorinated biphenyls in sediments and fish from freshwater cultured fish ponds in different agricultural contexts in north-eastern france.organochlorine pesticides (hcb, hch with α-, β-, and γ isomers, heptachlor, cis-heptachlor epoxyde, trans-heptachlor epoxyde, endosulfan with α- and β isomers, sulfate endosulfan, o,p'-ddt, p,p'-ddt, o,p'-dde, p,p'-dde, o,p'-ddd, p,p'-ddd, chlorothalonil, alachlor, aldrin, dieldrin, methoxychlor, oxychlordane, chlordane with α- and γ isomers, p,p'-dicofol and o,p'-dicofol) and indicators pcbs (iupac nos. 28, 52, 101, 118, 138, 153, and 180) were studied both in sediments and muscles of farmed fi ...201222071217
generation of the first bac-based physical map of the common carp genome.common carp (cyprinus carpio), a member of cyprinidae, is the third most important aquaculture species in the world with an annual global production of 3.4 million metric tons, accounting for nearly 14% of the all freshwater aquaculture production in the world. apparently genomic resources are needed for this species in order to study its performance and production traits. in spite of much progress, no physical maps have been available for common carp. the objective of this project was to genera ...201122044723
effects of long-term exposure to simazine in real concentrations on common carp (cyprinus carpio l.).the effects of a 90 day simazine exposure at concentrations of 0.06 (reported concentration in czech rivers), 1, 2, and 4 μg l⁻¹ were assessed in one-year-old common carp (cyprinus carpio l.). its influence on biometric parameters, hematology, blood biochemistry, liver biomarkers, and histology was investigated. biometric parameters of common carp exposed to simazine at 0.06 μg l⁻¹ showed no differences from untreated fish. simazine concentrations of 1, 2, and 4 μg l⁻¹ caused significant (p<0.01 ...201222036208
gill remodeling in crucian carp during sustained exercise and the effect on subsequent swimming performance.gill remodeling can be extensive in crucian carp, where up to a 7.5-fold increase in gill surface area has been observed during exposure to hypoxia through a reduction in the interlamellar cell mass (ilcm) and increased lamellar protrusion that has been hypothesized to be signaled by the need to maximize oxygen uptake under a given condition. sustained aerobic exercise may have the greatest influence on oxygen demand in fish; however, its effect on gill remodeling in crucian carp has not been in ...201122030846
distribution of cuo nanoparticles in juvenile carp (cyprinus carpio) and their potential toxicity.adverse effect of engineered nanoparticles (nps) on the aquatic environment and organisms has recently drawn much attention. this paper reports on the toxicity of cuo nps to juvenile carp (cyprinus carpio) and their distribution in the fish. cuo nps and its counterpart bulk particles (bps) (10, 50, 100, 200, 300, 500 and 1000 mg l(-1)) exhibited no acute toxicity (96 h), while during the 30 day sub-acute toxicity test, carp growth was significantly inhibited by cuo nps (100 mg l(-1)) in comparis ...201122014442
[correlation and path analyses of phenotypic traits and body mass of transgenic carp with growth hormone gene of salmon].thirty 2-year old transgenic carp individuals with growth hormone gene of salmon were randomly selected to study the affecting degree of their phenotypic traits on their body mass by the methods of correlation and path analyses, with 30 individuals of non-transgenic carp as the control, aimed to ascertain the main phenotypic parameters affecting the body mass of the transgenic and non-transgenic carps. the test phenotypic traits were total length, body length, body height, least height of caudal ...201122007470
modulation of cytochrome p450 and induction of dna damage in cyprinus carpio exposed in situ to surface water treated with chlorine or alternative disinfectants in different seasons.epidemiological studies have shown an association between consumption of disinfected drinking water and adverse health outcomes. the chemicals used to disinfect water react with occurring organic matter and anthropogenic contaminants in the source water, resulting in the formation of disinfection by-products (dbps). the observations that some dbps are carcinogenic in animal models have raised public concern over the possible adverse health effects for humans. here, the modulation of liver cytoch ...201222001235
comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring.exposure to estrogenic chemicals discharged into the aquatic environment has been shown to induce feminization in wild freshwater fish and although fish species have been reported to differ in their susceptibility for these effects, empirical studies that directly address this hypothesis are lacking. in this study, in vitro erα activation assays were applied in a range of fish species used widely in chemical testing (including, zebrafish, fathead minnow, medaka) and/or as environmental monitorin ...201222000336
autoantibodies recognizing carbamylated proteins are present in sera of patients with rheumatoid arthritis and predict joint damage.autoimmune responses against posttranslationally modified antigens are a hallmark of several autoimmune diseases. for example, antibodies against citrullinated protein antigens (acpa) have shown their relevance for the prognosis and diagnosis of rheumatoid arthritis (ra), and have been implicated in disease pathogenesis. it is conceivable that other autoantibody systems, recognizing other posttranslationally modified proteins, are also present in ra. here, we describe the presence of an autoanti ...201121987802
fish out of water: terrestrial jumping by fully aquatic fishes.many teleosts that live at the water's edge will voluntarily strand themselves to evade predators or escape poor conditions-this behavior has been repeatedly observed in the field for killifishes (cyprinodontiformes). although most killifishes are considered fully aquatic and possess no obvious morphological specializations to facilitate terrestrial locomotion, individuals from several different species have been observed moving across land via a "tail flip" behavior that generates a terrestrial ...201121972177
an observer study for a computer-aided reading protocol (carp) in the screening environment for digital mammography.the aims of this study were to investigate improving work flow efficiency by shortening the reading time of digital mammograms using a computer-aided reading protocol (carp) in the screening environment and to increase detection sensitivity using carp, compared to the current protocol, commonly referred to as the quadrant view (qv).201121971259
sequence of the pearl oyster carbonic anhydrase-related protein and its evolutionary implications.carbonic anhydrases are conserved in vertebrates and invertebrates, and a noncatalytic carbonic anhydrase-related protein viii (carp viii) has been found in deuterostomes and the phylum placozoa. i isolated a cdna encoding a noncatalytic carp from the mantle of the pearl oyster pinctada fucata. the polypeptide (carp-1) predicted from the nucleotide sequence shares 44-60% identity with known carp viii sequences, and its phylogenetic analysis showed that p. fucata formed a single group with deuter ...201221964518
comparative study of mercury accumulation in two fish species, (cyprinus carpio and sander lucioperca) from anzali and gomishan wetlands in the southern coast of the caspian sea.anzali and gomishan wetlands are considered as two of the most important wetlands in southern coast of caspian sea. to investigate mercury accumulation in these ecosystems, total mercury concentrations were measured in the muscle tissue of two fish species. higher mercury concentrations were detected in c. carpio, an omnivorous benthic/pelagic species (anzali wetland: 0.2 μg g(-1) wet weight; gomishan wetland: 0.2 μg g(-1) wet weight), than in s. lucioperca, a carnivorous pelagic species (anzali ...201121956764
accumulation of ²⁴¹am by crucian carp from food and water. 201621953235
immunotoxicity of 1-methyl-3-octylimidazolium bromide on brocarded carp (cyprinus carpio l.).in the present study, the immunotoxicity of 1-methyl-3-octylimidazolium bromide ([c(8)mim]br) on brocarded carp was evaluated by an acute exposure of 100-300mgl(-1) of [c(8)mim]br for 7 days. the results showed 300mgl(-1) of [c(8)mim]br exposure caused activity inhibition of specific and non-specific immune systems, mainly including igm level, lysozyme activity, and complement c3 content, while 100mgl(-1) of [c(8)mim]br activated fish immune system during the early periods of exposure (2-5 days) ...201221944958
reprint of: concentrations, distribution, and bioaccumulation of synthetic musks in the haihe river of china.seven typical synthetic musks (sms) in the samples from the surface water, sediment and fish of the haihe river were measured. the sm concentrations in the sediment and surface water of the haihe river were significantly lower than those in the dagu drainage river and chentaizi drainage river (p<0.05). along the flow direction, the sm concentrations in surface water and sediment tended to increase from the upstream to the downstream of dagu drainage river. the bioaccumulation factors (bafs) of g ...201121944040
toxicological responses of cyprinus carpio exposed to a commercial formulation containing glyphosate.the effects of commercial glyphosate herbicide formulation on the activity of acetylcholinesterase (ache) enzyme and oxidative stress were studied in cyprinus carpio exposed for 96 h to 0.0, 0.5, 2.5, 5.0 and 10.0 mg/l and then allowed to equal recovery period in water without herbicide. the activity of ache was inhibited in the brain and in the muscle after exposure. however, after recovery period brain and muscle ache activity increased. brain thiobarbituric acid reactive species (tbars) were ...201121931962
decabromodiphenyl ether (bde-209) enters the food web of the river po and is metabolically debrominated in resident cyprinid fishes.decabromodiphenyl ether (bde-209), the primary constituent of a widely used flame retardant formulation, is present at relatively high levels in sediments and macroinvertebrates of the river po. since it was demonstrated that bde-209 can be biotransformed to smaller and more toxic polybrominated dipheyl ethers (pbdes), the main objective of this study was to assess whether the large quantities of bde-209 present in the river po are bioavailable to the higher levels of the food web and are biotra ...201121925710
sublethal toxicity of esbiothrin relationship with total antioxidant status and in vivo genotoxicity assessment in fish (cyprinus carpio l., 1758) using the micronucleus test and comet assay.esbiothrin, synthetic pyrethroid with quick activity against insects, is widely used against household pests and in public health. despite widespread use, data on ecotoxicity and genotoxic effects are extremely scarce. the aim of the present study is to evaluate the genotoxic potential of esbiothrin on a model fish species cyprinus carpio l., 1758 (pisces: cyprinidae, koi) using the micronucleus test and comet assay in peripheral blood erythrocytes. effects of two sublethal exposure concentratio ...201321913301
identification of common carp innate immune genes with whole-genome sequencing and rna-seq data.the common carp is a candidate model system for immunology research. using next-generation sequencing technology, we have generated a huge amount of sequence reads from the carp genome and transcriptome. currently, our aim is to identify carp genes involved in the development of the innate immune response, particularly tir domain-containing genes, from a preliminary genome assembly. to achieve this, we developed a comprehensive gene identification pipeline. this analysis allowed us to estimate t ...201121908900
evaluations of the nutritional value of jatropha curcas protein isolate in common carp (cyprinus carpio l.).jatropha curcas seeds are rich in oil and protein. the oil is used for biodiesel production. jatropha seed cake (jsc) obtained after oil extraction is rich in protein; however, it is toxic (phorbol esters content 1.3 mg/g) and consists of 50-60% shells, which are indigestible. the principle of isoelectric precipitation was used to obtain jatropha protein isolate (jpi) from jsc and it was detoxified (djpi). carp (n = 45, 20.3 ± 0.13 g) were randomly distributed into five groups with three replica ...201221895778
principles of calcium-based biomineralization.the chapter provides some basic information on the formation principles of calcium carbonate in biological systems in marine environment in the point of view of materials science in order to provide strategies for biomimetic design and preparation of new functional materials. many researchers try to explain the principles of biomineralization and get some valuable conclusions. this chapter introduces some calcium-based biominerals in aquatic organisms which mainly include calcium carbonate and c ...201121877266
a genetic linkage map and comparative genome analysis of common carp (cyprinus carpio l.) using microsatellites and snps.a genetic linkage map is a powerful research tool for mapping traits of interest and is essential to understanding genome evolution. the aim of this study is to provide an expanded genetic linkage map of common carp to effectively carry out quantitative trait loci analysis and conduct comparative mapping analysis between lineages. here, we constructed a genetic linkage map of common carp (cyprinus carpio l.) using microsatellite and single-nucleotide polymorphism (snp) markers in a 159 sibling f ...201121870156
tissue distribution of dechlorane plus and its dechlorinated analogs in contaminated fish: high affinity to the brain for anti-dp.information on tissue distribution of dechlorane plus (dp) and its dechlorinated analogs in wildlife is scarce. dp isomers and two dechlorinated compounds, anti-cl(11)-dp and anti-cl(10)-dp, were examined in the muscle, liver, and brain tissues of two bottom fish species collected from an electronic waste recycling site, south china. the median levels of syn-, anti-, and anti-cl(11)-dp isomers in the tissues ranged 0.18-39.1, 0.22-52.9, and 0.01-5.63 ng/g wet wt, respectively. anti-cl(10)-dp was ...201121864960
do temporal and geographical patterns of hbcd and pbde flame retardants in u.s. fish reflect evolving industrial usage?polybrominated diphenyl ethers (pbdes) and hexabromocyclododecane (hbcd) are common flame retardants in polymers and textiles. recognition of the persistent, bioaccumulative, and toxic properties of pbdes has prompted reductions in their use. in contrast, hbcd has received less scrutiny. the u.s has historically been a dominant bfr consumer. however, the few publications on hbcd in wildlife here suggest modest levels compared to asian and european studies. in contrast, the hbcd concentrations we ...201121863854
[study on contamimation of endocrine disrupting chemicals in aquatic environment of qiantang river].to study contamination of endocrine disrupting chemicals (edcs) in aquatic environment of qiantang river.201121861354
effects of biologically-active chemical mixtures on fish in a wastewater-impacted urban stream.stream flow in urban aquatic ecosystems often is maintained by water-reclamation plant (wrp) effluents that contain mixtures of natural and anthropogenic chemicals that persist through the treatment processes. in effluent-impacted streams, aquatic organisms such as fish are continuously exposed to biologically-active chemicals throughout their life cycles. the north shore channel of the chicago river (chicago, illinois) is part of an urban ecosystem in which >80% of the annual flow consists of e ...201121849205
management implications of the response of two tilapiine cichlids to long-term changes in lake level, allodiversity and exploitation in an equatorial lake.the tilapiine cichlids oreochromis leucostictus and tilapia zillii were introduced into lake naivasha, kenya, in 1956. previous studies on data collected to 1987 revealed they were persistent following establishment, despite environmental variability and exploitation. recent data, however, suggest this persistence is under threat as data indicate some significant declines in aspects of their abundance since 1999. the influence of changes in lake level, allodiversity and fishing effort on this de ...201121848136
can pharmaceuticals interfere with the synthesis of active androgens in male fish? an in vitro study.the in vitro interference of fibrate (gemfibrozil, clofibrate, clofibric acid), anti-inflammatory (ibuprofen, diclofenac), and anti-depressive (fluoxetine, fluvoxamine) drugs with key enzymatic activities-c17,20-lyase and cyp11β-involved in the synthesis of active androgens in gonads of male carp have been investigated. among the tested compounds, fluvoxamine and fluoxetine were the strongest inhibitors of c17,20-lyase and cyp11β enzymes, with ic50s in the range of 321-335 μm and 244-550 μm, res ...201121820682
extending the use of spanish computer-assisted anomia rehabilitation program (carp-2) in people with aphasia.to extend the use of the spanish computer-assisted anomia rehabilitation program (carp-2) for anomia from a single case to a group of 15 people with aphasia. to evaluate whether the treatment is active (phase 1) for this group (robey & schultz, 1998), providing potential explanations as to why.201421794873
effects of atrazine and chlorpyrifos on acetylcholinesterase and carboxylesterase in brain and muscle of common carp.the aim of this study was to investigate the effects of chlorpyrifos (cpf), atrazine (atr) and the mixture of them on acetylcholinesterase (ache) and carboxylesterase (cbe) in brain and muscle of common carp, respectively. 220 carps were averagely divided into 11 groups according to the different treatments and concentration, including the exposure and recovery experiments. the activities of ache and cbe of the brain and muscle were determined at the end of the exposure and the recovery. the res ...201021787625
cytonuclear evidence for hybridogenetic reproduction in natural populations of the australian carp gudgeon (hypseleotris: eleotridae).although most vertebrates reproduce sexually, a small number of fishes, amphibians and reptiles are known in which reproduction is asexual, i.e. without meiotic recombination. in fishes, these so-called unisexual lineages usually comprise only females and utilize co-occurring males of a related sexual species to reproduce via gynogenesis or hybridogenesis. here, we examine patterns of microsatellite and mitochondrial dna (mtdna) variation in a widespread group of freshwater fishes (carp gudgeons ...201121777320
the development of myxobolus pavlovskii (myxozoa: myxobolidae) includes an echinactinomyxon-type actinospore.echinactinomyxon-type actinospores were found in a mixed-species oligochaete culture originating from the temperate water fish hatchery near budapest, hungary. on the basis of dna sequence analysis, the actinospores were identified as myxobolus pavlovskii (akhmerov, 1954), the 18s rdna sequence from myxospores of which is available in genbank. silver carp hypophthalmichthys molitrix (valenciennes) fry specimens were successfully infected by cohabitation with the echinactinomyxon-releasing oligoc ...201121776895
a memetic algorithm for periodic capacitated arc routing problem.this paper investigates the periodic capacitated arc routing problem (pcarp), which is often encountered in the waste collection application. pcarp is an extension of the well-known capacitated arc routing problem (carp) from a single period to a multi-period horizon. pcarp is a hierarchical optimization problem which has a primary objective (minimizing the number of vehicles ) and a secondary objective (minimizing the total cost ). an important factor that makes pcarp challenging is that its pr ...201121768050
biochemical markers of contamination in fish toxicity tests.markers of xenobiotic metabolization (cytochrome p450, ethoxyresorufin-o-deethylase, glutathione and glutathione-s-transferase) were investigated in the liver of the common carp cyprinus carpio after 28-day exposure to different pesticide formulations.the fish exposed to herbicide sencor 70 wg (metribuzin 700 g/kg) of 0.25 and 2.5 mg/l showed no change in cytochrome p450 and activity of ethoxyresorufin-o-deethylase when compared to control.successor 600 (pethoxamid 600 g/l) of 0.06; 0.22 and 0.6 ...201121753904
elevated serum igg4 is associated with chronic antibiotic-refractory pouchitis.we recently reported mucosal infiltration of igg4-expressing plasma cells in a patient with chronic antibiotic-refractory pouchitis (carp). the role of serum igg4 in the pathogenesis and clinical course of ileal pouch disorders has not been investigated. we hypothesized that igg4-mediated autoimmunity may be a contributing factor in for carp. the aims of the study were to investigate the prevalence of elevated serum igg4 in symptomatic patients with ileal pouches and to characterize clinical fea ...201121725838
resetting our priorities in environmental health: an example from the south-north partnership in lake chapala, mexico.lake chapala is a major source of water for crop irrigation and subsistence fishing for a population of 300,000 people in central mexico. economic activities have created increasing pollution and pressure on the whole watershed resources. previous reports of mercury concentrations detected in fish caught in lake chapala have raised concerns about health risks to local families who rely on fish for both their livelihood and traditional diet. our own data has indicated that 27% of women of childbe ...201121722889
in vivo treatment with progestogens causes immunosuppression of carp cyprinus carpio leucocytes by affecting nitric oxide production and arginase activity.in this study, carp cyprinus carpio were injected with various steroid compounds, including synthetic and natural progestogens and the glucocorticoid cortisol, to investigate effects on leucocytes isolated from their kidneys. injection of cortisol led to an increased spleeno-somatic index (i(s)) on day 21 post-injection (pi) and immunosuppressive effects measured as decreased nitric oxide (no) production and increased arginase activity in isolated leucocytes on days 14 and 21 pi, respectively. m ...201121722110
cloning, expression and functional characterization of carp, cyprinus carpio, estrogen receptors and their differential activations by estrogens.sex-steroid hormones are essential for normal reproductive activity in both sexes. estrogens are necessary for ovarian differentiation during a critical developmental stage in vertebrates and promote the growth and differentiation of the female reproductive system. importantly, environmental estrogens can influence the reproductive system and have been shown to disrupt gametogenesis in males. to understand the molecular mechanisms of estrogen actions and to evaluate estrogen receptor ligand inte ...201321721020
prevalence and clinical implications of positive serum anti-microsomal antibodies in symptomatic patients with ileal pouches.autoimmune disorders (aid) have been shown to be associated with chronic antibiotic-refractory pouchitis (carp). the role of anti-microsomal antibodies in ileal pouch disorders has not been investigated. the aims of the study were to investigate the prevalence of positive anti-microsomal antibody in symptomatic patients with ileal pouches and to investigate its clinical implications.201121717284
effects of ph on copper accumulation and toxicity in the common carp, cyprinus carpio.static, short-term acute toxicity tests were performed over a period of 96h, using different concentrations of copper on common carp (cyprinus carpio) fingerlings (3.2 +/- 1.1 cm and 0.42 g +/- 0.2 g) at ph 6.5, ph 7.5 and ph 8.5. the effect of copper on carp was significantly high (determined as lc50) at ph 6.5 followed by ph 7.5 and ph 8.5. the lethal toxic effect of copper on common carp was directly proportional to ph. exposure to sub-lethal doses of 0.25, 0.3, 0.35, 0.45 ppm for 15 days ind ...201123029935
comparison of nitrofen uptake via water and food and its distribution in tissue of common carp, cyprinus carpio l.carp (cyprinus carpio l.) were exposed to nitrofen (nip) by different routes (via water or food) to compare bioaccumulation parameters and tissue distribution. the bioconcentration factor of nip was 5,100, and the lipid-corrected biomagnification factor was 0.137. growth-corrected elimination half lives were 2.1-3.0 days via aqueous exposure and 2.7-2.9 days via dietary exposure. from either uptake route, the tissue distribution of nip was highest in the head, followed by muscle, viscera, dermis ...201121713387
are fish immune systems really affected by parasites? an immunoecological study of common carp (cyprinus carpio).the basic function of the immune system is to protect an organism against infection in order to minimize the fitness costs of being infected. according to life-history theory, energy resources are in a trade-off between the costly demands of immunity and other physiological demands. concerning fish, both physiology and immunity are influenced by seasonal changes (i.e. temporal variation) associated to the changes of abiotic factors (such as primarily water temperature) and interactions with path ...201121708010
defining global gene expression changes of the hypothalamic-pituitary-gonadal axis in female sgnrh-antisense transgenic common carp (cyprinus carpio).the hypothalamic-pituitary-gonadal (hpg) axis is critical in the development and regulation of reproduction in fish. the inhibition of neuropeptide gonadotropin-releasing hormone (gnrh) expression may diminish or severely hamper gonadal development due to it being the key regulator of the axis, and then provide a model for the comprehensive study of the expression patterns of genes with respect to the fish reproductive system.201121695218
antioxidant properties of peptide fractions from silver carp (hypophthalmichthys molitrix) processing by-product protein hydrolysates evaluated by electron spin resonance spectrometry.silver carp processing by-product protein is usually discarded as an industrial solid waste. in this study the protein was recovered using a ph-shift method, after which seven commercial proteases were separately employed to prepare antioxidative hydrolysates. among the hydrolysates, pepsin hydrolysates, which had the highest free radical-scavenging activity, were further separated into five peptide fractions, scph-i (>10kda), scph-ii (5-10kda), scph-iii (3-5kda), scph-iv (1-3kda), and scph-v (< ...201125213938
transmural inflammation is not pathognomonic for crohn's disease of the pouch.transmural inflammation shown by imaging and histology has been considered a hallmark of crohn's disease (cd). however, the diagnostic and prognostic value of this feature in cd of the pouch has not been evaluated. this study aimed to evaluate the clinical utility of transmural inflammation in patients with ileal pouch-anal anastomosis (ipaa) using in vivo optical coherence tomography (oct) and histopathology.201121660630
hypoxia-induced oxidative dna damage links with higher level biological effects including specific growth rate in common carp, cyprinus carpio l.both hypoxia and hyperoxia, albeit in different magnitude, are known stressors in the aquatic environment. adopting an integrated approach, mirror carp (cyprinus carpio l.), were exposed chronically (i.e. 30 days) to hypoxic (1.8 ± 1.1 mg o(2) l(-1)) and hyperoxic (12.3 ± 0.5 mg o(2) l(-1)) conditions and resultant biological responses or biomarkers were compared between these two treatments as well as with fish held under normoxic conditions (7.1 ± 1.04 mg o(2) l(-1)). the biomarkers determined ...201121656038
polar metabolites synergize the activity of prostaglandin f2α in a species-specific hormonal sex pheromone released by ovulated common carp.many species of teleost fish detect and release f prostaglandins (pgfs), but the specific identities of these compounds and how they function as species-specific pheromones have yet to be resolved. this study addressed these questions in the common carp. an initial set of experiments established that mature male common carp were attracted to chemicals released by ovulated conspecifics, whereas the odor of female goldfish, a close relative, was less attractive. tests of fractionated holding water ...201121647722
molecular characterization of cart, agrp, and mc4r genes and their expression with fasting and re-feeding in common carp (cyprinus carpio).cocaine and amphetamine-regulated transcript (cart), agouti-related proteins (agrp) and melanocortin 4 receptor (mc4r) involves in the control of appetite. the genes were cloned and characterized, and their regulation was studied in common carp. the carti and cartii genes encode 117- and 120-amino acids, respectively. the agrp-1 and agrp-2 genes encode 128- and 136-amino acids, respectively. carti was principally expressed in the brain, eye and ovary, while cartii was highly expressed in the bra ...201221643748
adrenergic regulation of the innate immune response in common carp (cyprinus carpio l.).catecholamines exert their physiological actions through α and β adrenergic receptors (ars). as ars are not exclusively expressed on neuroendocrine cells, but also on leukocytes, they may facilitate neuroendocrine modulation of immune responses. we sequenced the β(2a)-ar in common carp, and studied its expression profile and involvement in the regulation of teleost innate immune responses. β(2a)-ar messenger rna was found to be constitutively expressed in brain areas, especially in the preoptic ...201221641927
assessment of the genotoxic impact of pesticides on farming communities in the countryside of santa catarina state, brazil.the aim of this study was to assess the use of pesticides on farms located in the lambedor river watershed in guatambu, state of santa catarina, as well as to determine, by micronucleus testing, the risk of genotoxic impact. samples from locally collected cyprinus carpio, hypostomus punctatus, rhamdia quelen and oreochromis niloticus gave evidence of a mean increase in micronuclei frequency from 6.21 to 13.78 in 1,000 erythrocytes, a clear indication of the genotoxic potenciality of pesticide re ...201121637554
pilot investigation of perfluorinated compounds in river water, sediment, soil and fish in tianjin, china.detectable pfcs could be found in all samples. perfluorooctanoic acid (pfoa) was the major pfc in river water, while perfluorooctane sulfonate (pfos) was dominant in sediment and were 17- to 153-fold higher than those in water. pfcs concentrations in soil were little higher than those in sediment. in fish muscles pfos showed the highest concentrations. generally, pfc concentrations in fish were in the following rank order: crucian carp > silver carp> common carp. we suggested that there may be c ...201121626112
concentrations, distribution, and bioaccumulation of synthetic musks in the haihe river of china.seven typical synthetic musks (sms) in the samples from the surface water, sediment and fish of the haihe river were measured. the sm concentrations in the sediment and surface water of the haihe river were significantly lower than those in the dagu drainage river and chentaizi drainage river (p<0.05). along the flow direction, the sm concentrations in surface water and sediment tended to increase from the upstream to the downstream of dagu drainage river. the bioaccumulation factors (bafs) of g ...201121624633
redescription and molecular analysis of myxobolus shantungensis hu, 1965 (myxozoa: myxosporea) infecting common carp cyprinus carpio haematopterus.myxobolus shantungensis hu, 1965 infects gill arches of common carp cyprinus carpio haematopterus (temminck and schlegel), causing serious pathological effects on host fish. an inadequate original description and absence of molecular data make accurate early diagnosis challenging. to augment the original description, m. shantungensis is redescribed here using morphological and molecular biological methods. mature spores of m. shantungensis were ellipsoidal or apple shaped in frontal view and lem ...201121614546
chemoprotection of lipoic acid against microcystin-induced toxicosis in common carp (cyprinus carpio, cyprinidae).this paper evaluated the chemoprotective effect of lipoic acid (la) against microcystin (mc) toxicity in carp cyprinus carpio. to determine the la dose and the time necessary for the induction of three different classes (alpha, mu and pi) of glutathione s-transferase (gst) gene transcription, carp were i.p. injected with 40mg/kg lipoic acid solution. a group was killed 24h after the first i.p. injection (condition 1); another group received two i.p. injections with a 24h of interval between each ...201121586338
glycophorin in red blood cell membranes of healthy and diseased carp, cyprinus carpio l. 201121585396
effect of photoperiod on growth and feed conversion of juvenile wild carp, cyprinus carpio.because of the effect of photoperiod on physiological and biochemical processes in fish, this study aimed to evaluate the effect of manipulated photoperiod on growth, feed conversion and survival of wild carp, cyprinus carpio. fish received six photoperiod regimes (light:dark cycle) including: natural photoperiod (control), 24l:0d, 16l:8d, 12l:12d, 8l:16d and 0l:24d by the three replications. regulated photoperiods as a 16l:8d or 12l:12d light/dark cycle significantly improved growth rate and fo ...201121555274
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