| identification of a 43-kda outer membrane protein of fusobacterium necrophorum that exhibits similarity with pore-forming proteins of other fusobacterium species. | a pair of primers was designed in an attempt to amplify outer membrane protein (omp) gene of fusobacterium necrophorum based on nucleotide sequence of the omp of fusobacterium nucleatum. further analysis was performed to characterize its molecular properties and phylogeny in the genus fusobacterium. we identified a predicated 43kda outer membrane protein (43k omp) in f. necrophorum, which showed the same properties as other pore-forming proteins of gram-negative anaerobic bacteria according to a ... | 2013 | 23433684 |
| infections caused by fusobacterium in children: a 14-year single-center experience. | this study aimed at reviewing our experience with infections caused by fusobacterium in children. | 2015 | 25929419 |
| lemierre's syndrome: a rare case of pulmonic valve vegetation. | lemierre's syndrome is an uncommon complication of pharyngitis commonly associated with an anaerobic gram negative bacterium, fusobacterium necrophorum. the syndrome usually affects young healthy adults with the mean age of 20 and is characterized by recent pharyngitis followed by ipsilateral internal jugular vein thrombosis and septic thromboembolism. the treatment is at least 6 weeks of antibiotics; the role of anticoagulation is unclear. the following presentation is a case of lemierre's synd ... | 2013 | 23573433 |
| [lemierre's syndrome as differential diagnosis of lung cancer]. | lemierre's syndrome is a disseminated infection which is usually caused by fusobacterium necrophorum. an oropharyngeal infection progresses to a septic thrombophlebitis of the internal jugular vein and later metastatic infections throughout the body occur. we present a clinical case in which a patient, initially presenting with symptoms characteristic of pulmonary cancer, turned out to have a rare variant of lemierre's syndrome caused by fusobacterium nucleatum. | 2012 | 22668649 |
| microbial colonization at the implant-abutment interface and its possible influence on periimplantitis: a systematic review and meta-analysis. | the aim of this systematic review and meta-analysis was to evaluate the microbial colonization at the implant-abutment interfaces (iai) on bone-level implants and to identify possible association with peri-implant conditions. | 2017 | 28359872 |
| inhibition of the mura enzyme in fusobacterium nucleatum by potential inhibitors identified through computational and in vitro approaches. | fusobacterium nucleatum plays a key role in several diseases such as periodontitis, gingivitis, appendicitis, and inflammatory bowel disease (ibd). the development of antibiotic resistance by this bacterium demands novel therapeutic intervention. our recent study has reported udp-n-acetylglucosamine 1-carboxyvinyltransferase (mura) as one of the potential target proteins in f. nucleatum. in this study, we proposed two novel mura inhibitors through in silico screening and evaluated their mode of ... | 2017 | 28358152 |
| tea polyphenols inhibit the growth and virulence properties of fusobacterium nucleatum. | fusobacterium nucleatum plays a key role in creating the pathogenic subgingival biofilm that initiates destructive periodontitis. it is also a common resident of the human gastrointestinal tract and has been associated with inflammatory bowel disease. the aim of the present study was to investigate the effects of green and black tea extracts as well as two of their bioactive components, egcg and theaflavins, on the growth and virulence properties of f. nucleatum. the tea extracts and components ... | 2017 | 28322293 |
| changes in salivary microbiota increase volatile sulfur compounds production in healthy male subjects with academic-related chronic stress. | to investigate the associations among salivary bacteria, oral emanations of volatile sulfur compounds, and academic-related chronic stress in healthy male subjects. | 2017 | 28319129 |
| pyogenic liver abscess due to fusobacterium nucleatum in a patient with liver hemangiomas. | | 2017 | 28317637 |
| effect of gallium-arsenic laser on photosensitized periodontopathic anaerobic organisms: an in vitro study. | the mainstay of periodontal therapy is mechanical removal of subgingival plaque. there is considerable interest in supplementing it with the use of antibiotics and antiseptics. many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. hence, alternate means of killing subgingival bacteria are clearly desirable. one such method is the use of laser. | 2017 | 28298816 |
| carcinogenesis mechanisms of fusobacterium nucleatum. | transformed cells of cancers may be related to stromal cells, immune cells, and some bacteria such as fusobacterium nucleatum. this review aimed to evaluate carcinogenesis mechanisms of fusobacterium spp. in the oral cavity, pancreatic and colorectal cancers. these cancers are the three of the ten most prevalence cancer in the worldwide. recent findings demonstrated that f. nucleatum could be considered as the risk factor for these cancers. the most important carcinogenesis mechanisms of f. nucl ... | 2017 | 28292019 |
| quantitative proteomic analysis of extracellular matrix extracted from mono- and dual-species biofilms of fusobacterium nucleatum and porphyromonas gingivalis. | the gram-negative bacteria fusobacterium nucleatum and porphyromonas gingivalis are members of a complex dental biofilm associated with periodontal disease. in this study, we cultured f. nucleatum and p. gingivalis as mono- and dual-species biofilms, and analyzed the protein composition of the biofilms extracellular polymeric matrix (epm) by high-resolution liquid chromatography-tandem mass spectrometry. label-free quantitative proteomic analysis was used for identification of proteins and seque ... | 2017 | 28285095 |
| community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients. | mechanically ventilated patients are at risk for developing ventilator-associated pneumonia, and it has been reported that dental plaque provides a reservoir of respiratory pathogens that may aspirate to the lungs and endotracheal tube (ett) biofilms. for the first time, metataxonomics was used to simultaneously characterize the microbiome of dental plaque, etts, and non-directed bronchial lavages (nbls) in mechanically ventilated patients to determine similarities in respective microbial commun ... | 2017 | 28259058 |
| [microbial biomarkers for early cancer detection]. | the human microbiome - the vast amount of microbes that colonize our body - play an important role in maintaining our health. changes in microbiome composition have been linked to multiple diseases including cancer. although mechanisms and causalities of these associations still have to be uncovered, microbiome alterations across various stages of disease can be utilized for novel diagnostic and prognostic tests. research on biomarkers extracted from the gut microbiome has in particular focused ... | 2017 | 28235227 |
| the role of il-1 gene polymorphisms (il1a, il1b, and il1rn) as a risk factor in unsuccessful implants retaining overdentures. | implant-supported overdentures are an alternative predictable rehabilitation method that has a high impact on improving the patient's quality of life. however, some biological complications may interfere with the maintenance and survival of these overdenture implants. the goal of this article was to assess the factors that affect peri-implant success, through a hypothetical prediction model for biological complications of implant overdentures. | 2017 | 28223139 |
| structure of the lps o-chain from fusobacterium nucleatum strain 10953, containing sialic acid. | fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. recently, it has been gaining attention as a potential causative agent for colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. little is known about virulence factors of this organism and thus we have initiated studies to examine the bacterial surface glycochemistry. consistent with a recent paper suggesting that f. nucleatum strain 1059 ... | 2017 | 28199859 |
| in vitro evaluation of the biological compatibility and antibacterial activity of a bone substitute material consisting of silver-doped hydroxyapatite and bio-oss(®). | this study evaluated biological compatibility and antibacterial activity of a bone substitute material consisting of silver-doped hydroxyapatite (agha) and bio-oss(®) with different mixture ratios in vitro and investigated its antibacterial mechanism. agha was synthesized by a chemical precipitation method. after characterization, agha was mixed with bio-oss(®) at three ratios: 1:1, 1:2, and 1:4 by weight. then, porphyromonas gingivalis (pg) and fusobacterium nucleatum (fn) were used to test the ... | 2017 | 28186709 |
| identification and characterization of a novel fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with streptococcus gordonii. | to successfully colonize the oral cavity, bacteria must directly or indirectly adhere to available oral surfaces. fusobacterium nucleatum plays an important role in oral biofilm community development due to its broad adherence abilities, serving as a bridge between members of the oral biofilm that cannot directly bind to each other. in our efforts to characterize the molecular mechanisms utilized by f. nucleatum to physically bind to key members of the oral community, we investigated the involve ... | 2017 | 28173636 |
| fusobacterium nucleatum bacteremia presenting with portal vein thrombosis: an abdominal lemierre syndrome? | | 2017 | 28163049 |
| mining the proteome of fusobacterium nucleatum subsp. nucleatum atcc 25586 for potential therapeutics discovery: an in silico approach. | the plethora of genome sequence information of bacteria in recent times has ushered in many novel strategies for antibacterial drug discovery and facilitated medical science to take up the challenge of the increasing resistance of pathogenic bacteria to current antibiotics. in this study, we adopted subtractive genomics approach to analyze the whole genome sequence of the fusobacterium nucleatum, a human oral pathogen having association with colorectal cancer. our study divulged 1,499 proteins o ... | 2016 | 28154519 |
| tumor potentiating mechanisms of fusobacterium nucleatum, a multifaceted microbe. | | 2017 | 28143770 |
| the structure of the lps o-chain of fusobacterium nucleatum strain 25586 containing two novel monosaccharides, 2-acetamido-2,6-dideoxy-l-altrose and a 5-acetimidoylamino-3,5,9-trideoxy-gluco-non-2-ulosonic acid. | fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. recently, it has been gaining attention as a potential causative agent for colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. little is known about the virulence factors of this organism, and thus we have initiated studies to examine the bacterium's surface glycochemistry. we isolated lipopolysaccharide (lps) from f. nucleatum strain 25 ... | 2017 | 28135570 |
| association of dietary patterns with risk of colorectal cancer subtypes classified by fusobacterium nucleatum in tumor tissue. | fusobacterium nucleatum appears to play a role in colorectal carcinogenesis through suppression of the hosts' immune response to tumor. evidence also suggests that diet influences intestinal f nucleatum. however, the role of f nucleatum in mediating the relationship between diet and the risk of colorectal cancer is unknown. | 2017 | 28125762 |
| oral microbiome and history of smoking and colorectal cancer. | the equilibrium of oral microbiome may be altered by environmental factors, including cigarette smoking. several recent studies also suggest that oral pathogens causing periodontal disease, such as fusobacterium nucleatum, are involved in pathogenesis of colorectal cancer. | 2016 | 28111632 |
| antibacterial efficacy of triple-layered poly(lactic-co-glycolic acid)/nanoapatite/lauric acid guided bone regeneration membrane on periodontal bacteria. | a guided bone regeneration (gbr) membrane has been extensively used in the repair and regeneration of damaged periodontal tissues. one of the main challenges of gbr restoration is bacterial colonization on the membrane, constitutes to premature membrane degradation. therefore, the purpose of this study was to investigate the antibacterial efficacy of triple-layered gbr membrane composed of poly(lactic-co-glycolic acid) (plga), nanoapatite (nap) and lauric acid (la) with two types of gram-negativ ... | 2017 | 28111388 |
| sequential colonization of periodontal pathogens in induction of periodontal disease and atherosclerosis in ldlrnull mice. | periodontal disease (pd) and atherosclerotic vascular disease (asvd) are both chronic inflammatory diseases with a polymicrobial etiology and have been epidemiologically associated. the purpose is to examine whether periodontal bacteria that infect the periodontium can also infect vascular tissues and enhance pre-existing early aortic atherosclerotic lesions in ldlrnull mice. mice were orally infected with intermediate bacterial colonizer fusobacterium nucleatum for the first 12 weeks followed b ... | 2017 | 28104616 |
| novel reuterin-related compounds suppress odour by periodontopathic bacteria. | halitosis is caused by volatile sulphur compounds including methyl mercaptan (ch3 sh) in the oral cavity and is a serious problem that limits interpersonal social communication. the aim of study was to evaluate the effects of reuterin-related compounds (rrcs) on halitosis-related periodontopathic bacteria in vitro. | 2017 | 28083982 |
| detection of fusobacterium nucleatum in two cases of empyema and lung abscess using paromomycin-vancomycin supplemented brucella hk agar. | fusobacterium nucleatum was found in patients with empyema or pulmonary abscess, using paromomycin-vancomycin brucella hk agar. in vitro examination revealed that growth of the strains differed significantly in different media. clinicians should be aware that suboptimal f. nucleatum cultivation methods may result in an underestimation of its frequency. | 2017 | 28034636 |
| selective persistence of propionibacterium species fma5 following sealing of infected dentinal matrix. | the objective of the present study was to test the hypothesis that nutrient deprivation by effective isolation should inactivate causative saccharolytic bacteria occupying carious lesions. vital maxillary third molar teeth were prepared by removing only the superficial necrotic material, leaving behind infected dentinal matrix, before the cavity was sealed with glass ionomer cement (gic). before sealing, lesions were biopsied to provide reference bacterial dna for microbial analysis. after an in ... | 2016 | 28013413 |
| comparative study on the characteristics of weissella cibaria cmu and probiotic strains for oral care. | probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. the present work was conducted to compare the characteristics of oral care probiotics: weissella cibaria cmu (chonnam medical university) and four commercial probiotic strains. survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and i ... | 2016 | 27999400 |
| streptococcus mutans spap binds to radd of fusobacterium nucleatum ssp. polymorphum. | adhesin-mediated bacterial interspecies interactions are important elements in oral biofilm formation. they often occur on a species-specific level, which could determine health or disease association of a biofilm community. among the key players involved in these processes are the ubiquitous fusobacteria that have been recognized for their ability to interact with numerous different binding partners. fusobacterial interactions with streptococcus mutans, an important oral cariogenic pathogen, ha ... | 2016 | 27976528 |
| fusobacterium nucleatum: an unusual cause of subdural empyema. | this article describes an uncommon presentation of a subdural empyema in a young man. because the patient's initial presentation consisted of nonspecific abdominal complaints, subdural empyema was not initially considered. however, a thorough medical history, including recent travel history, and appropriate multidisciplinary consultation, were key to reaching the correct diagnosis. | 2016 | 27898560 |
| quantitative detection of periodontopathic bacteria in lower respiratory tract specimens by real-time pcr. | the presence of common periodontopathic bacteria, the fusobacterium nucleatum-periodonticum-simiae group, prevotella intermedia, and porphyromonas gingivalis was determined from respiratory tract specimens of bacterial pneumonia by real-time pcr using universal and species-specific taqman probe/primer sets. 42 patients with infectious pneumonia and 45 patients without infectious pneumonia were retrospectively enrolled in clinical studies. periodontopathic bacterial dna was found in 57.1% cases o ... | 2017 | 27894820 |
| porphyromonas gingivalis capsule-mediated coaggregation as a virulence factor in mixed infection with fusobacterium nucleatum. | porphyromonas gingivalis capsule enables evasion from phagocytosis, invasion of keratinocytes and bacterial survival. in mixed infection, the capsule also participates in coaggregation, which may lead to characteristic virulence not present in the mono-infection. the aim of the study was to evaluate the role of p.gingivalis capsule as a virulence factor in co-aggregated mixed infection with fusobacterium nucleatum. | 2016 | 27885964 |
| fusobacterium nucleatum increases proliferation of colorectal cancer cells and tumor development in mice by activating toll-like receptor 4 signaling to nuclear factor-κb, and up-regulating expression of microrna-21. | nearly 20% of the global cancer burden can be linked to infectious agents. fusobacterium nucleatum promotes tumor formation by epithelial cells via unclear mechanisms. we aimed to identify micrornas (mirnas) induced by f nucleatum and evaluate their ability to promote colorectal carcinogenesis in mice. | 2017 | 27876571 |
| inducible expression of a disintegrin and metalloproteinase 8 in chronic periodontitis and gingival epithelial cells. | the expression of a disintegrin and metalloproteinase 8 (adam8) is associated with several inflammatory diseases. elevated adam8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. the objective of this study was to investigate adam8 expression in chronic periodontitis tissues compared with that in normal tissues. adam8 expression and its inductive mechanism were examined in human gingival epithelial cells (hgecs) and human gingival fibroblasts. | 2016 | 27859260 |
| cross-sectional evaluation of periodontal status and microbiologic and rheumatoid parameters in a large cohort of patients with rheumatoid arthritis. | this study evaluates periodontal conditions and microbiologic findings and their influence on rheumatologic disease parameters in patients with rheumatoid arthritis (ra). | 2017 | 27858553 |
| fusobacterium nucleatum in colorectal carcinoma tissue according to tumor location. | evidence suggests a possible role of fusobacterium nucleatum in colorectal carcinogenesis, especially in right-sided proximal colorectum. considering a change in bowel contents and microbiome from proximal to distal colorectal segments, we hypothesized that the proportion of colorectal carcinoma enriched with f. nucleatum might gradually increase along the bowel subsites from rectum to cecum. | 2016 | 27811909 |
| effects by periodontitis on pristane-induced arthritis in rats. | an infection-immune association of periodontal disease with rheumatoid arthritis has been suggested. this study aimed to investigate the effect of pre-existing periodontitis on the development and the immune/inflammatory response of pristane-induced arthritis. | 2016 | 27809921 |
| | background nonsurgical periodontal treatment implicates bacteraemia. in rare cases oral pathogens can be found associated with abscesses of brain or liver case description a brain abscess was found in a patient after several periodontal treatments causing neurological seizures. in the drain masses of fusobacterium nucleatum were found. an oral examination revealed a severe periodontally damaged dentition. the woman had been in a generally healthy condition before the event. after neurosurgical t ... | 2016 | 27808349 |
| quantitation of faecal fusobacterium improves faecal immunochemical test in detecting advanced colorectal neoplasia. | there is a need for an improved biomarker for colorectal cancer (crc) and advanced adenoma. we evaluated faecal microbial markers for clinical use in detecting crc and advanced adenoma. | 2016 | 27797940 |
| human microbiome fusobacterium nucleatum in esophageal cancer tissue is associated with prognosis. | fusobacterium nucleatum (f. nucleatum) is a component of the human microbiome that primarily inhabits the oral cavity. it causes periodontal disease and has also been implicated in the development of human cancers. although there are several reports of the relationship between f. nucleatum and the clinical outcome in human cancers, its prognostic significance in esophageal cancer remains unclear. | 2016 | 27769987 |
| consequences of orthodontic treatment in malocclusion patients: clinical and microbial effects in adults and children. | malocclusion is a common disease of oral and maxillofacial region. the study was aimed to investigate levels changes of periodontal pathogens in malocclusion patients before, during and after orthodontic treatments, and to confirm the difference between adults and children. | 2016 | 27793138 |
| [analysis of causes and whole microbial structure in a case of rampant caries]. | to analyze the whole microbial structure in a case of rampant caries to provide evidence for its prevention and treatment. | 2016 | 27777193 |
| strain-specific impact of fusobacterium nucleatum on neutrophil function. | neutrophil function is critical for initiation and progression of infecto-inflammatory diseases. key quorum-sensing plaque bacteria, such as fusobacterium nucleatum, act as bridging species between early and late colonizer pathogens, such as porphyromonas gingivalis, as the biofilm ages and periodontal inflammation increases. this study is designed to determine impact of different f. nucleatum strains on neutrophil function. | 2017 | 27762731 |
| efficacy of various side-to-side toothbrushes and impact of brushing parameters on noncontact biofilm removal in an interdental space model. | the objective of this study was to evaluate the efficacy of four different side-to-side toothbrushes and the impact of various brushing parameters on noncontact biofilm removal in an adjustable interdental space model. | 2016 | 27757550 |
| light activated antimicrobial agents can inactivate oral malodour causing bacteria. | oral malodour is a common condition which affects a large proportion of the population, resulting in social, emotional and psychological stress. certain oral bacteria form a coating called a biofilm on the tongue dorsum and degrade organic compounds releasing volatile sulfur compounds that are malodourous. current chemical treatments for oral malodour such as mouthwashes containing chlorhexidine or essential oils, are not sufficiently effective at reducing the bacterial load on the tongue. one p ... | 2016 | 27753430 |
| tea polyphenols inhibit the activation of nf-κb and the secretion of cytokines and matrix metalloproteinases by macrophages stimulated with fusobacterium nucleatum. | fusobacterium nucleatum has been associated with both periodontal disease and inflammatory bowel disease. this gram-negative bacterium possesses a high inflammatory potential that may contribute to the disease process. we hypothesized that green and black tea polyphenols attenuate the inflammatory response of monocytes/macrophages mediated by f. nucleatum. we first showed that the tea extracts, egcg and theaflavins reduce the nf-κb activation induced by f. nucleatum in monocytes. since nf-κb is ... | 2016 | 27694921 |
| role of oral microbiome on oral cancers, a review. | the oral cavity is inhibited by many of the bacterial species. some of them have a key role in the development of oral disease. interrelationships between oral microbiome and systemic conditions such as head-and-neck cancer have become increasingly appreciated in recent years. emerging evidence also suggests a link between periodontal disease and oral cancer, and the explanation being that chronic inflammation could be a major factor in both diseases. squamous cell carcinoma is that the most fre ... | 2016 | 27693964 |
| evaluation of antibody level against fusobacterium nucleatum in the serological diagnosis of colorectal cancer. | fusobacterium nucleatum (f. nucleatum, fn) is associated with the colorectal cancer (crc). fn-infection could induce significant levels of serum fn-specific antibodies in human and mice. the objective of this study was to identify fn-infection that elicit a humoral response in patients with crc and evaluate the diagnostic performance of serum anti-fn antibodies. in this work, we showed the mean absorbance value of anti-fn-iga and -igg in the crc group were significantly higher than those in the ... | 2016 | 27678333 |
| antibacterial and antibiofilm activities of docosahexaenoic acid (dha) and eicosapentaenoic acid (epa) against periodontopathic bacteria. | docosahexaenoic acid (dha) and eicosapentaenoic acid (epa) are two major omega-3 polyunsaturated fatty acids (n-3 pufas) with antimicrobial properties. in this study, we evaluated the potential antibacterial and antibiofilm activities of dha and epa against two periodontal pathogens, porphyromonas gingivalis (p. gingivalis) and fusobacterium nucleatum (f. nucleatum). mtt assay showed that dha and epa still exhibited no cytotoxicity to human oral tissue cells when the concentration came to 100 μm ... | 2016 | 27565090 |
| bacterial colonization of the peri-implant sulcus in dentate patients: a prospective observational study. | the aim of the present study was to compare the composition of the periodontal microflora at baseline (t0) with the submucosal microflora at least 1 year after implant placement (t1) in periodontally healthy patients. | 2017 | 27558381 |
| comparative genome analysis of fusobacterium nucleatum. | fusobacterium nucleatum is considered to be a key oral bacterium in recruiting periodontal pathogens into subgingival dental plaque. currently f. nucleatum can be subdivided into five subspecies. our previous genome analysis of f. nucleatum w1481 (referred to hereafter as w1481), isolated from an 8-mm periodontal pocket in a patient with chronic periodontitis, suggested the possibility of a new subspecies. to further investigate the biology and relationships of this possible subspecies with othe ... | 2016 | 27540086 |
| oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study. | polymorphonuclear neutrophils (pmns) have a major role in the innate immune system. however, little is known about pmn contribution in relation to oral health. the objective of this study was to investigate the numbers and functional characteristics of oral pmns (opmns) compared with circulatory pmns (cpmns). oral rinse and venous blood samples were obtained from 268 systemically and orally healthy volunteers in a cross-sectional observational study. pmn counts, cell cycle analysis and cellular ... | 2016 | 27515277 |
| fap2 mediates fusobacterium nucleatum colorectal adenocarcinoma enrichment by binding to tumor-expressed gal-galnac. | fusobacterium nucleatum is associated with colorectal cancer and promotes colonic tumor formation in preclinical models. however, fusobacteria are core members of the human oral microbiome and less prevalent in the healthy gut, raising questions about how fusobacteria localize to crc. we identify a host polysaccharide and fusobacterial lectin that explicates fusobacteria abundance in crc. gal-galnac, which is overexpressed in crc, is recognized by fusobacterial fap2, which functions as a gal-gal ... | 2016 | 27512904 |
| [intramedullary abscess of the cervical spinal cord caused by advanced periodontitis:case report]. | we describe the case of a 60-year-old man with an intramedullary abscess of the cervical spinal cord caused by advanced periodontitis. he suddenly developed severe neck pain and rapidly progressive palsy of the left upper arm. t2-weighted sagittal magnetic resonance imaging(mri)revealed a hyperintense area extending from c1 to c6. gadolinium-enhanced t1-weighted mri showed a ring-enhanced lesion at the c3-4 level that was hyperintense on diffusion-weighted mri. the patient underwent drainage of ... | 2016 | 27506846 |
| bacteria prevalence in a large italian population sample: a clinical and microbiological study. | the present study detects those bacterial species which are more strongly related to bleeding on probing, suppuration and smoking in periodontal-affected patients. nine hundred and fifty-one patients with periodontal diseases were admitted to the department of periodontology and implantology, dental school of bologna university where they underwent microbiological tests for six periodontal pathogens (actinomyces actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, treponema de ... | 2016 | 27469569 |
| impact of implant-abutment connection on osteoimmunological and microbiological parameters in short implants: a randomized controlled clinical trial. | the study aimed to determine the levels of soluble receptor activator of nuclear factor-кb ligand (srankl) and osteoprotegerin (opg) as well as their relative calculated ratio in peri-implant crevicular fluid (picf) obtained around two different types of implant-abutment connection on short implants following a 12-month monitoring period. moreover, the levels of a number of oral bacterial species were investigated in the corresponding submucosal biofilm samples. | 2016 | 27469171 |
| anti-biofilm activities from resveratrol against fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative, anaerobic bacterium that plays an important role in dental plaque biofilm formation. in this study, we evaluate the effect of resveratrol, a phytoalexin compound, on f. nucleatum biofilm formation. the effects of different concentrations of resveratrol on biofilms formed on 96-well microtiter plates at different time points were determined by the mtt assay. the structures and thicknesses of the biofilm were observed by confocal laser scanning microscop ... | 2016 | 27458454 |
| broad-range pcr coupled with electrospray ionization time of flight mass spectrometry for detection of bacteremia and fungemia in patients with neutropenic fever. | infection is an important complication in patients with hematologic malignancies or solid tumors undergoing intensive cytotoxic chemotherapy. in only 20 to 30% of the febrile neutropenic episodes, an infectious agent is detected by conventional cultures. in this prospective study, the performance of broad-range pcr coupled with electrospray ionization time of flight mass spectrometry (pcr/esi-ms) technology was compared to conventional blood cultures (bc) in a consecutive series of samples from ... | 2016 | 27440820 |
| alteration of neutrophil reactive oxygen species production by extracts of devil's claw (harpagophytum). | harpagophytum, devil's claw, is a genus of tuberiferous xerophytic plants native to southern africa. some of the taxa are appreciated for their medicinal effects and have been traditionally used to relieve symptoms of inflammation. the objectives of this pilot study were to investigate the antioxidant capacity and the content of total phenols, verbascoside, isoverbascoside, and selected iridoids, as well as to investigate the capacity of various harpagophytum taxa in suppressing respiratory burs ... | 2016 | 27429708 |
| differential matrix metalloprotease (mmp) expression profiles found in aged gingiva. | the periodontium undergoes age-related cellular and clinical changes, but the involved genes are not yet known. here, we investigated age-related genetic changes in gingiva at the transcriptomic level. genes that were differentially expressed between young and old human gingiva were identified by rna sequencing and verified by real-time pcr. a total of 1939 mrna transcripts showed significantly differential expression between young and old gingival tissues. matrix metalloprotease (mmp) regulatio ... | 2016 | 27391467 |
| bone remodeling around implants with external hexagon and morse-taper connections: a randomized, controlled, split-mouth, clinical trial. | to evaluate clinical, radiographic, microbiologic, and biomechanical parameters related to bone remodeling around implants with external hexagon (eh) and morse-taper (mt) connections. | 2017 | 27353076 |
| rapid detection of nusg and fada in fusobacterium nucleatum by loop-mediated isothermal amplification. | fusobacterium nucleatum is associated with various human diseases such as periodontal disease and colorectal cancer (crc); thus, f. nucleatum detection might serve as a novel diagnostic tool. here, we describe the development of a sensitive and rapid molecular method for detecting two f. nucleatum genes: the highly conserved nusg and fada, which encode a critical host colonization factor. loop-mediated isothermal amplification (lamp) primer sets for the rapid detection of nusg and fada were desi ... | 2016 | 27339262 |
| caveolin-1 serves as a negative effector in senescent human gingival fibroblasts during fusobacterium nucleatum infection. | it is well established that aging is associated with increased susceptibility to infectious diseases. fusobacterium nucleatum is a well-known bacterial species that plays a central bridging role between early and late colonizers in the human oral cavity. further, the ability of f. nucleatum to invade gingival fibroblasts (gfs) is critical to the development of periodontal diseases. however, the mechanisms underlying the age-related infection of gfs by f. nucleatum remain unknown. we used young ( ... | 2016 | 27315395 |
| metabolic and community synergy of oral bacteria in colorectal cancer. | the oral periodontopathic bacterium fusobacterium nucleatum has been repeatedly associated with colorectal tumors. molecular analysis has identified specific virulence factors that promote tumorigenesis in the colon. however, other oral community members, such as members of the porphyromonas spp., are also found with f. nucleatum on colonic tumors, and thus, narrow studies of individual pathogens do not take community-wide virulence properties into account. a broader view of oral bacterial physi ... | 2017 | 27303740 |
| molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease. | dogs commonly serve as a model for various human conditions, including periodontal diseases. the aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (rt-pcr)-based tests and to compare the results obtained in each species. bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. five samples were c ... | 2017 | 27297417 |
| 5-fluorouracil sensitivity varies among oral micro-organisms. | 5-fluorouracil (5-fu), a commonly used chemotherapeutic agent, often causes oral mucositis, an inflammation and ulceration of the oral mucosa. micro-organisms in the oral cavity are thought to play an important role in the aggravation and severity of mucositis, but the mechanisms behind this remain unclear. although 5-fu has been shown to elicit antibacterial effects at high concentrations (>100 µm), its antibacterial effect at physiologically relevant concentrations in the oral cavity is unknow ... | 2016 | 27296270 |
| 24-hour evaluation of dental plaque bacteria and halitosis after consumption of a single placebo or dental treat by dogs. | objective to determine whether consumption of a single dental treat with specific mechanical properties and active ingredients would provide a 24-hour effect on dental plaque bacteria and halitosis in dogs. animals 10 dogs of various breeds from a privately owned colony that had received routine dental scaling and polishing 4 weeks before the study began. procedures dogs were randomly assigned to receive 1 placebo or dental treat first. a 4-week washout period was provided, and then dogs receive ... | 2016 | 27227499 |
| global tlr2 and 4 deficiency in mice impacts bone resorption, inflammatory markers and atherosclerosis to polymicrobial infection. | toll-like-receptors (tlrs) play a significant role in the generation of a specific innate immune response against invading pathogens. tlr2 and tlr4 signaling contributes to infection-induced inflammation in periodontal disease (pd) and atherosclerosis. observational studies point towards a relationship between pd and atherosclerosis, but the role of tlr2 and tlr4 in the recognition of multiple oral pathogens and their modulation of host response leading to atherosclerosis are not clear. we evalu ... | 2016 | 27224005 |
| fusobacterium nucleatum: atypical organism of pyogenic liver abscess might be related to sigmoid diverticulitis. | pyogenic liver abscesses (plas) are the most common form of liver abscesses in the united states. most cases are caused by enteric bacteria and anaerobes. we report a case of pla caused by a rare pathogen, fusobacterium nucleatum, from an unusual primary site of infection. | 2016 | 27213146 |
| endothelial cell response to fusobacterium nucleatum. | vascular response is an essential aspect of an effective immune response to periodontal disease pathogens, as new blood vessel formation contributes to wound healing and inflammation. gaining a greater understanding of the factors that affect vascular response may then contribute to future breakthroughs in dental medicine. in this study, we have characterized the endothelial cell response to the common bacterium fusobacterium nucleatum, an important bridging species that facilitates the activity ... | 2016 | 27185790 |
| neutrophil superoxide release and plasma c-reactive protein levels pre- and post-periodontal therapy. | to determine peripheral blood neutrophil superoxide release and c-reactive protein (crp) concentration in chronic periodontitis patients, before and after non-surgical periodontal treatment. | 2016 | 27168055 |
| microbial colonization of the peri-implant sulcus and implant connection of implants restored with cemented versus screw-retained superstructures: a cross-sectional study. | the aim of this study is to investigate peri-implant and intraconnection microflora of healthy implants restored with cemented and screwed superstructures. | 2016 | 27153291 |
| a case of liver abscess with systemic infection caused by fusobacterium. | a 43-year-old man was admitted to our hospital because of an abnormal shadow on chest x-ray. blood testing showed elevated levels of c-reactive protein and white blood cells. computed tomography revealed multilocular masses of the right hepatic lobe, reticulonodular shadowing on both lungs, left kidney masses, and aortic arch aneurysm. fusobacterium nucleatum was isolated from the hepatic abscess after percutaneous transhepatic drainage. because of severe dental caries, he was diagnosed with liv ... | 2016 | 27151479 |
| effects of lactoferrin and lactoperoxidase-containing food on the oral hygiene status of older individuals: a randomized, double blinded, placebo-controlled clinical trial. | lactoferrin and lactoperoxidase have antimicrobial effects against oral pathogens. this randomized, double-blinded, placebo-controlled parallel group study tested the efficacy of a lactoferrin and lactoperoxidase-containing tablet (lf + lpo tablet) in improving the oral hygiene status of older individuals. | 2016 | 27150460 |
| invasive fusobacterium nucleatum may play a role in the carcinogenesis of proximal colon cancer through the serrated neoplasia pathway. | the prevalence of invasive fusobacterium nucleatum (fn) within the serrated neoplasia pathway of the proximal colon has seldom been investigated. we examined the invasive fn and bacterial biofilms in 35 proximal hyperplastic polyps (hps), 33 sessile serrated adenomas (ssas), 48 proximal colorectal cancers (crcs) and 10 matched metastatic lymph nodes using 16s rrna fluorescence in situ hybridization (fish). samples of normal mucosa, traditional adenomas (tas), distal hps, distal crcs and matched ... | 2016 | 27130618 |
| highly sensitive stool dna testing of fusobacterium nucleatum as a marker for detection of colorectal tumours in a japanese population. | background accumulating evidence shows an over-abundance of fusobacterium nucleatum in colorectal tumour tissues. although stool dna testing of fusobacterium nucleatum might be a potential marker for the detection of colorectal tumours, the difficulty in detecting fusobacterium nucleatum in stool by conventional methods prevented further explorations. therefore, we developed a droplet digital polymerase chain reaction (pcr) assay for detecting fusobacterium nucleatum in stool and investigated it ... | 2017 | 27126270 |
| copaifera reticulata oleoresin: chemical characterization and antibacterial properties against oral pathogens. | oral infections such as periodontitis and tooth decay are the most common diseases of humankind. oleoresins from different copaifera species display antimicrobial and anti-inflammatory activities. copaifera reticulata is the commonest tree of this genus and grows abundantly in several brazilian states, such as pará, amazonas, and ceará. the present study has evaluated the chemical composition and antimicrobial potential of the copaifera reticulata oleoresin (cro) against the causative agents of ... | 2016 | 27118478 |
| two foxp3(+)cd4(+) t cell subpopulations distinctly control the prognosis of colorectal cancers. | cd4(+) t cells that express the forkhead box p3 (foxp3) transcription factor function as regulatory t (treg) cells and hinder effective immune responses against cancer cells. abundant treg cell infiltration into tumors is associated with poor clinical outcomes in various types of cancers. however, the role of treg cells is controversial in colorectal cancers (crcs), in which foxp3(+) t cell infiltration indicated better prognosis in some studies. here we show that crcs, which are commonly infilt ... | 2016 | 27111280 |
| nox1/2 activation in human gingival fibroblasts by fusobacterium nucleatum facilitates attachment of porphyromonas gingivalis. | periodontal diseases are infectious polymicrobial inflammatory diseases that lead to destruction of the periodontal ligament, gingiva, and alveolar bone. sequential colonization of a broad range of bacteria, including fusobacterium nucleatum and porphyromonas gingivalis, is an important phenomenon in this disease model. f. nucleatum is a facultative anaerobic species thought to be a key mediator of dental plaque maturation due to its extensive coaggregation with other oral bacteria, while p. gin ... | 2016 | 27071620 |
| in vitro evaluation of planktonic growth on experimental cement-retained titanium surfaces. | background the purpose of this study was to compare the effects of selected cements, or their combination with titanium, on the growth of two periodontopathic bacteria: prevotella intermedia (pi) and fusobacterium nucleatum (fn). material and methods this study was comprised of several experimental groups: 1) dental luting cements (glass ionomer cement, methacrylate-based resin cement, zinc-oxide eugenol cement, eugenol-free zinc oxide cement; 2) titanium discs; and 3) titanium combination cemen ... | 2016 | 27058704 |
| comparison of fusobacterium nucleatum and porphyromonas gingivalis lipopolysaccharides clinically isolated from root canal infection in the induction of pro-inflammatory cytokines secretion. | the aim of this study was to compare the biological activity of lipopolysaccharides (lps) purified from fusobacterium nucleatum and porphyromonas gingivalis strains, both isolated from primary endodontic infection (pei) in the levels of il-1β and tnf-α released by macrophage cells. moreover, lps was purified from f. nucleatum and p. gingivalis american type collection (atcc) and its biological activity was compared to respectively clinical isolates strains. f. nucleatum and p. gingivalis strains ... | 2017 | 27058385 |
| microbiota-based model improves the sensitivity of fecal immunochemical test for detecting colonic lesions. | colorectal cancer (crc) is the second leading cause of death among cancers in the united states. although individuals diagnosed early have a greater than 90% chance of survival, more than one-third of individuals do not adhere to screening recommendations partly because the standard diagnostics, colonoscopy and sigmoidoscopy, are expensive and invasive. thus, there is a great need to improve the sensitivity of non-invasive tests to detect early stage cancers and adenomas. numerous studies have i ... | 2016 | 27056827 |
| high-throughput dental biofilm growth analysis for multiparametric microenvironmental biochemical conditions using microfluidics. | dental biofilm formation is not only a precursor to tooth decay, but also induces more serious systematic health problems such as cardiovascular disease and diabetes. understanding the conditions promoting colonization and subsequent biofilm development involving complex bacteria coaggregation is particularly important. in this paper, we report a high-throughput microfluidic 'artificial teeth' device offering controls of multiple microenvironmental factors (e.g. nutrients, growth factors, dissol ... | 2016 | 27045372 |
| a dual-species microbial model for studying the dynamics between oral streptococci and periodontal pathogens during biofilm development on titanium surfaces by flow cytometry. | the association of the pioneer organisms streptococcus mutans atcc 25175 or streptococcus oralis atcc 9811 with secondary colonizers fusobacterium nucleatum atcc 25586 or porphyromonas gingivalis atcc 33277 during biofilm development on titanium surfaces was evaluated by flow cytometry (fcm) using specific polyclonal antibodies. elisa and fcm were employed, revealing high antibody sensitivity and specificity. biofilm formation of four dual-species combinations was analyzed by crystal violet stai ... | 2016 | 27032997 |
| association of fusobacterium nucleatum infection with colorectal cancer in chinese patients. | to investigate fusobacterium nucleatum (f. nucleatum) abundance in colorectal cancer (crc) tissues and its association with crc invasiveness in chinese patients. | 2016 | 27004000 |
| effect of alendronate on the progression of periodontitis induced by porphyromonas gingivalis and fusobacterium nucleatum: a study in rats. | the study aimed to investigate the effect of alendronate (aln) on the inhibition of alveolar bone loss in experimental periodontitis in wistar rats. | 2016 | 26955836 |
| [association between oral conditions and colorectal cancer: a literature review and synthesis]. | chronic periodontitis and tooth loss are chronic oral conditions that, in recent decades, have been implicated in the occurrence of certain types of cancer. in this review, we address the question of whether colorectal cancer is associated with these oral conditions. | 2016 | 26923863 |
| morphological and functional adaptations of fusobacterium nucleatum exposed to human neutrophil peptide-1. | we recently demonstrated that fusobacterium nucleatum can resist to human neutrophil peptide (hnp)-1 by decreasing its membrane permeability and increasing its proliferation and biofilm formation. in this continuation study, we aimed to further evaluate and explain these resistance properties by determining the morphological and functional adaptations of f. nucleatum, using transmission electron microscopy (tem). | 2016 | 26923748 |
| registered report: fusobacterium nucleatum infection is prevalent in human colorectal carcinoma. | the reproducibility project: cancer biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. the papers, which were published between 2010 and 2012, were selected on the basis of citations and altmetric scores (errington et al., 2014). this registered report describes the proposed replication plan of key experiments from 'fusobacterium nucleatum in ... | 2016 | 26882501 |
| symptoms of periodontitis and antibody responses to porphyromonas gingivalis in juvenile idiopathic arthritis. | the association between rheumatoid arthritis (ra) and periodontitis is well established. some children with juvenile idiopathic arthritis (jia) phenotypically resemble adults with ra, characterized by the presence of anti-cyclic citrullinated peptide (ccp) antibodies. we sought to investigate an association between ccp-positive jia and symptoms of periodontitis and antibodies to oral microbiota. | 2016 | 26861944 |
| case series of patients with fusobacterium nucleatum bacteremia with emphasis on the presence of cancer. | fusobacterium nucleatum is anaerobic oral microbiota that might be associated with cancer. we reported 22 consecutive cases of patients (mean age of 63.8 years (range 34-89), 59.1% male) with f. nucleatum bacteremia that were admitted to a university hospital over a 10-year period. in 17 (77.2%) of these patients, f. nucleatum was the sole possible pathogen. seven of the 22 patients (31.8%) had active cancer: esophagus carcinoma (n = 3), hematologic malignancies (n = 1), gastrointestinal stromal ... | 2016 | 26853831 |
| periodontitis induced by porphyromonas gingivalis drives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune response. | to identify a causal mechanism responsible for the enhancement of insulin resistance and hyperglycaemia following periodontitis in mice fed a fat-enriched diet. | 2017 | 26838600 |
| association of fusobacterium nucleatum with immunity and molecular alterations in colorectal cancer. | the human intestinal microbiome plays a major role in human health and diseases, including colorectal cancer. colorectal carcinogenesis represents a heterogeneous process with a differing set of somatic molecular alterations, influenced by diet, environmental and microbial exposures, and host immunity. fusobacterium species are part of the human oral and intestinal microbiota. metagenomic analyses have shown an enrichment of fusobacterium nucleatum (f. nucleatum) in colorectal carcinoma tissue. ... | 2016 | 26811607 |
| fusobacterium nucleatum infections: clinical spectrum and bacteriological features of 78 cases. | few series describe the clinical spectrum of fusobacterium spp. infections. among them, fewer discuss f. nucleatum, even though there are many clinical cases. | 2016 | 26783023 |
| selective responses of human gingival fibroblasts and bacteria on carbon fiber reinforced polyetheretherketone with multilevel nanostructured tio2. | the long-term success of dental implants relies not only on stable osseointegration but also on the integration of implant surfaces with surrounding soft tissues. in our previous work, titanium plasma immersion ion implantation (piii) technique was applied to modify the carbon-fiber-reinforced polyetheretherketone (cfrpeek) surface, constructing a unique multilevel tio2 nanostructure thus enhancing certain osteogenic properties. however, the interactions between the modified surface and soft-tis ... | 2016 | 26774566 |
| effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility. | periodontitis is a chronic inflammatory disease induced by a biofilm that forms on the tooth surface. increased periodontal disease is associated with aging. we investigated the effect of aging on challenge by oral pathogens, examining the host response, colonization, and osteoclast numbers in aged versus young mice. we also compared the results with mice with lineage-specific deletion of the transcription factor foxo1, which reduces dendritic cell (dc) function. periodontitis was induced by ora ... | 2016 | 26762510 |
| fusobacterium nucleatum, inflammation, and immunity: the fire within human gut. | fusobacterium nucleatum is an identified proinflammatory autochthonous bacterium implicated in human colorectal cancer. it is also abundantly found in patients suffering from chronic gut inflammation (inflammatory bowel disease), consequently contributing to the pathogenesis of colorectal cancer. majority of the studies have reported that colorectal tumors/colorectal adenocarcinomas are highly enriched with f. nucleatum compared to noninvolved adjacent colonic tissue. during the course of multis ... | 2016 | 26718210 |
| fusobacterium nucleatum infection of gingival epithelial cells leads to nlrp3 inflammasome-dependent secretion of il-1β and the danger signals asc and hmgb1. | fusobacterium nucleatum is an invasive anaerobic bacterium that is associated with periodontal disease. previous studies have focused on virulence factors produced by f. nucleatum, but early recognition of the pathogen by the immune system remains poorly understood. although an inflammasome in gingival epithelial cells (gecs) can be stimulated by danger-associated molecular patterns (damps) (also known as danger signals) such as atp, inflammasome activation by this periodontal pathogen has yet t ... | 2016 | 26687842 |