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cloning and characterization of photobacterium damselae ssp. piscicida phospholipase: an enzyme that shows haemolytic activity.a phospholipase gene of photobacterium damselae ssp. piscicida (ppp) was cloned from a genomic library and its nucleotide sequence was determined. the open reading frame consisted of 1218 bp encoding a protein of 405 amino acids with a predicted molecular mass of 46 kda. the ppp had identities (53-55%) with phospholipase and haemolysin of vibrio spp., while it showed low identities (23-26%) with glycerophospholipid cholesterol acyltransferase of aeromonas spp. a recombinant ppp (rppp) with a his ...200717958612
analysis of type iii effector genes in typical and atypical aeromonas salmonicida. 200717958615
tracheobronchitis caused by aeromonas veronii biovar sobria after near-drowning.a 19-year-old man developed an acute tracheobronchitis shortly after having been rescued from a near-drowning in a river where previous investigations had demonstrated the presence of 500 c.f.u. ml(-1) of aeromonas sp. in the water. an isolate of aeromonas veronii biovar sobria was identified as the causative agent of the tracheobronchitis. the causality was supported by the massive growth of a. veronii in bronchial secretion, the presence of a type iii secretion system in the bacterial isolate, ...200717965361
[factors of virulence associated with enteropathogenicity in strains of aeromonas spp. isolated from children with diarrhea in mérida, venezuela].the feces of 397 patients with acute diarrheal disease (add) and of other 121 patients without diarrea (control group) were studied in the state of mérida, venezuela, from june 1993 to december 1994. the genus aeromonas was identified in patients with add in 11.83% and in 5.78% of the patients from the control group. on studying the virulence factors described for aeromonas (enterotoxin, cytotoxin, hemaglutinins, cellular hydrofibrosity, and hemolytic activity) in the isolated strains, it was de ...200517966577
cloning, expression and characterization of aerolysin from aeromonas hydrophila in escherichia coli.aerolysin is a toxin (protein in nature) secreted by the strains of aeromonas spp. and plays an important role in the virulence of aeromonas strains. it has also found several applications such as for detection of glycosylphosphatidylinositol (gpi)-anchored proteins etc. a. hydrophila is a ubiquitous gram-negative bacterium which causes frequent harm to the aquaculture. to obtain a significant amount of recombinant aerolysin in the active form, in this study, we expressed the aerolysin in e. col ...200717970277
febrile response to infection in the american alligator (alligator mississippiensis).temperature probes were inserted into the stomachs of juvenile american alligators (alligator mississippiensis) maintained outdoors at ambient fluctuating temperatures. internal body temperatures (t(b)) were measured every 15 min for two days, and then the alligators were injected with bacterial lipopolysaccharide (lps), pyrogen-free saline, or left untreated. alligators injected intraperitoneally with lps exhibited maximum t(b)s 2.6+/-1.1 degrees c and 3.5+/-1.2 degrees c higher than untreated ...200717977038
proteomic analysis of rainbow trout (oncorhynchus mykiss, walbaum) serum after administration of probiotics in diets.the response of rainbow trout (oncorhynchus mykiss, walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (apr). proteomic analysis by two-dimensional electrophoresis (2d) concurrently with mass spectrometry was used to detect apr related proteins in rainbow trout serum following feeding with probiotics aeromonas sobria gc2 and bacillus sp. jb-1. three candidate proteins increased following use of gc2, and wer ...200817981340
isolation of aeromonas salmonicida from sea lamprey (petromyzon marinus) with furuncle-like lesions in lake ontario.for the past six decades, parasitic sea lampreys (petromyzon marinus) have caused devastating losses to salmonid fisheries in the great lakes. to reduce the number of sea lampreys, the great lakes fishery commission began a large-scale program based on trapping male sea lampreys, sterilizing them, and releasing sterile males back into streams to compete with fertile males for spawning females. the transfer of lampreys among lakes can potentially lead to the transfer of various pathogens, and thi ...200717984256
aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains.aeromonads are inhabitants of aquatic ecosystems and are described as being involved in intestinal disturbances and other infections. a total of 200 drinking water samples from domestic and public reservoirs and drinking fountains located in são paulo (brazil), were analyzed for the presence of aeromonas. samples were concentrated by membrane filtration and enriched in apw. ada medium was used for aeromonas isolation and colonies were confirmed by biochemical characterization. strains isolated w ...200817998612
drinking water microbiological survey of the northwestern state of sinaloa, mexico.a potable water survey, in two important municipalities of the state of sinaloa, mexico was conducted. culiacan, capital city of sinaloa and its neighboring municipality, navolato were selected to enumerate aeromonas hydrophila, escherichia coli, fecal and total coliforms, pseudomonas aeruginosa, and heterotrophic plate count bacteria from 100 households' taps. manganese; residual chlorine; ph; temperature and turbidity were also examined. overall, aeromonas hydrophila was not detected in any of ...200817998613
a distinct cause of necrotizing fasciitis: aeromonas veronii biovar sobria.aeromonas veronii biovar sobria (veronii var. sobria) has not been implicated in the pathogenesis of necrotizing fasciitis in north america.200717999586
[neutropenic enterocolitis in adult patients with acute leukemia or stem cell transplant recipients: study of 7 cases].neutropenic enterocolitis (ne) is a complication arising in neutropenic patients with acute leukemia or solid tumours while treated with intensive chemotherapy. the optimal therapeutic procedures have not been well established.200718005634
dietary korean mistletoe enhances cellular non-specific immune responses and survival of japanese eel (anguilla japonica).the present study was performed to investigate the immunostimulatory effects of korean mistletoe extract (km-110; viscum album coloratum) on the non-specific immune response and protection against aeromonas hydrophila infection in japanese eel (anguilla japonica). eels were fed under 4 regimes, 0%, 0.1%, 0.5% and 1.0% km-110 mixed diet. on day 14 after feeding, 15 fish from each group were injected i.p. with live a. hydrophila (3 x 10(6)cfu) and the remaining unchallenged fish from each group we ...200818023593
sensitive and rapid detection of aeromonas caviae in stool samples by loop-mediated isothermal amplification.in this study, a loop-mediated isothermal amplification assay targeting the 16s-23s intergenic spacer regions (internal transcribed spacer) of aeromonas caviae was developed. eighteen reference strains and 109 clinical samples were analyzed. the results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods.200818024054
accumulation of silver(i) ion and diamine silver complex by aeromonas sh10 biomass.the biomass of aeromonas sh10 was proven to strongly absorb ag+ and [ag(nh3)2]+. the maximum uptake of [ag(nh3)2]+ was 0.23 g(ag) g(-1)(cell dry weight), higher than that of ag+. fourier transform infrared spectroscopy spectra analysis indicated that some organic groups, such as amide and ionized carboxyl in the cell wall, played an important role in the process of biosorption. after sh10 cells were suspended in the aqueous solution of [ag(nh3)2]+ under 60 degrees c for more than 12 h, [ag(nh3)2 ...200718025596
combination of n149s and d171g mutations in aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis.aeromonas caviae polyhydroxyalkanoate synthase (phac(ac)) is an important biocatalyst for the synthesis of practically useful two-component polyhydroxyalkanoate copolymer, poly[(r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate] [p(3hb-co-3hhx)]. in a previous study, two phac(ac) mutants that have a single amino acid substitution of either asparagine 149 by serine (n149s) or aspartate 171 by glycine (d171g) were isolated as higher active enzymes by means of evolutionary engineering. in this study, ...200718031343
aeromonas sobria prostatitis and septic shock in a healthy man with chronic alcoholic consumption.prostate infection caused by aeromonas sobria has not been reported in the literature. we presented a 44-year-old healthy man with a habit of alcoholic consumption who was admitted because of primary a. sobria prostatitis with septicemia. the patient was successfully treated with intravenous ofloxacin and was uneventful after a half-year follow-up. based on our rare case, we suggest to clinical physicians that prostatitis might be considered in healthy male patients with a. sobria bacteremia and ...200718032845
establishment and characterization of dual-species biofilms formed from a 3,5-dinitrobenzoic-degrading strain and bacteria with high biofilm-forming capabilities.in this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-dnba)-degrading bacterium, comamonas testosteroni a3, was investigated. our results showed that the combinations of strain a3 with each of five strains with a high biofilm-forming capability (pseudomonas sp. m8, pseudomonas putida m9, bacillus cereus m19, pseudomonas plecoglossicida m21 and aeromonas hydrophila m22) presented different l ...200818034834
development of a pcr protocol for the detection of aeromonas salmonicida in fish by amplification of the fsta (ferric siderophore receptor) gene.the aims of the study were to evaluate a new pcr protocol designed to detect aeromonas salmonicida in fish tissues and to develop a non-destructive method for the diagnosis of furunculosis. a set of primers (fer3, fer4), flanking a fragment of the fsta gene (coding for the ferric-siderophore receptor) was designed, showing to be sensitive and specific. when compared to pcr methods previously reported, the new protocol recognized all the 69 a. salmonicida strains evaluated, with no cross-reaction ...200818035507
molecular characterization of a functional type vi secretion system from a clinical isolate of aeromonas hydrophila.our laboratory recently molecularly characterized the type ii secretion system (t2ss)-associated cytotoxic enterotoxin (act) and the t3ss-secreted aexu effector from a diarrheal isolate ssu of aeromonas hydrophila. the role of these toxin proteins in the pathogenesis of a. hydrophila infections was subsequently delineated in in vitro and in vivo models. in this study, we characterized the new type vi secretion system (t6ss) from isolate ssu of a. hydrophila and demonstrated its role in bacterial ...200818037263
aeromonas hydrophila wound infection following a tiger bite in nepal.aeromonas hydrophila is a rare human pathogen. reports of zoonotic infection developing after large feline bites are even rarer. we are documenting the first case of human wound infection with a. hydrophila following a tiger bite. the patient responded well following wound debridement, secondary suturing and combination antibiotic therapy.200718041304
antibacterial activity of guava (psidium guajava l.) and neem (azadirachta indica a. juss.) extracts against foodborne pathogens and spoilage bacteria.the antibacterial activity of guava (psidium guajava) and neem (azadirachta indica) extracts against 21 strains of foodborne pathogens were determined--listeria monocytogenes (five strains), staphylococcus aureus (four strains), escherichia coli o157:h7 (six strains), salmonella enteritidis (four strains), vibrio parahaemolyticus, and bacillus cereus, and five food spoilage bacteria: pseudomonas aeroginosa, p. putida, alcaligenes faecalis, and aeromonas hydrophila (two strains). guava and neem e ...200718041957
arsenic-resistant bacteria isolated from contaminated sediments of the orbetello lagoon, italy, and their characterization.the aim of this study was to isolate arsenic-resistant bacteria from contaminated sediment of the orbetello lagoon, italy, to characterize isolates for as(iii), as(v), heavy metals resistance, and from the phylogenetic point of view.200718045414
isolation of a novel strain of aeromonas media producing high levels of dopa-melanin and assessment of the photoprotective role of the melanin in bioinsecticide applications.to isolate a bacterium that produces high yield of melanin and to examine the effect of this bacterial pigment on the efficacy of a bioinsecticide.200718045437
in vitro studies of lactobacillus delbrueckii subsp. lactis in atlantic salmon (salmo salar l.) foregut: tissue responses and evidence of protection against aeromonas salmonicida subsp. salmonicida epithelial damage.probiotic bacteria increase the host health status and protect mucosal tissue against pathogen-caused damage in mammalian models. using an in vitro (intestinal sac) method this study aimed to address (a) the in vitro ability of lactobacillus delbrueckii subsp. lactis to remain in the gastrointestinal tract of atlantic salmon (salmo salar l.) and (b) its ability to prevent cellular damage caused by successive incubation with aeromonas salmonicida subsp. salmonicida the causative agent of furuncul ...200818054448
antibacterial action of l-amino acid oxidase from the skin mucus of rockfish sebastes schlegelii.l-amino acid oxidase (lao) shows broadly antibacterial activity against gram-positive and gram-negative bacteria by h(2)o(2) generated in the oxidative process of l-amino acids. however, lao (termed ssap) isolated from the rockfish sebastes schlegelii skin mucus acted selectively on gram-negative bacteria. therefore, this study was undertaken to clarify the antibacterial action of ssap as compared with h(2)o(2). ssap inhibited potently the growth of aeromonas salmonicida, photobacterium damselae ...200818055243
the involvement of teta and tete tetracycline resistance genes in plasmid and chromosomal resistance of aeromonas in brazilian strains.this study analyzed the involvement of teta and tete genes in the tetracycline resistance of 16 strains of genus aeromonas, isolated from clinical and food sources. polymerase chain reactions revealed that 37.5% of the samples were positive for teta, and also 37.5% were tete positive. one isolate was positive for both genes. only the isolate a. caviae 5.2 had its resistance associated to the presence of a plasmid, pss2. the molecular characterization of pss2 involved the construction of its rest ...200718060315
enhanced innate immune parameters in labeo rohita (ham.) following oral administration of bacillus subtilis.the present study assessed the use of bacillus subtilis in fish as a probiotic. the bacterium was administered orally at three different doses 0.5 x 10(7) (t(2)), 1 x 10(7) (t(3)), 1.5 x 10(7) (t(4)) cfu/g feed to labeo rohita for two weeks. the positive control group (t(1)) and negative control group (t(5,)) were fed feed without b. subtilis for the same period. on the 15th day blood and serum were sampled to determine respiratory burst activity (nbt assay), differential leukocyte counts (dlc) ...200818060807
[microbial diversity in scorpion intestine (buthus martensii karsch)].scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ...200718062268
cortisol response and immune-related effects of atlantic salmon (salmo salar linnaeus) subjected to short- and long-term stress.it is generally considered that stress causes decreased immune function in fish. in this study we examined in atlantic salmon (salmo salar linnaeus) the effects of both short- (a single 15s out of water) and long-term (4 weeks of daily handling 15s out of water) stress on plasma cortisol (free and total) and glucose levels, expression of interleukin-1beta (il-1beta) and survival of head kidney (hk) macrophages under culture with aeromonas salmonicida. in the short-term study, samples were collec ...200818065240
quorum sensing by n-acylhomoserine lactones is not required for aeromonas hydrophila during growth with organic particles in lake water microcosms.it was investigated whether quorum sensing (qs) mediated by n-acylhomoserine lactones (ahls) was important for heterotrophic bacteria from the littoral zone of the oligotrophic lake constance for growth with organic particles. more than 900 colonies from lake water microcosms with artificial organic aggregates consisting of autoclaved unicellular algae embedded in agarose beads were screened for ahl-production. ahl-producing bacteria of the genus aeromonas enriched in the microcosms but ahls cou ...200818066531
[identification of closely related bacteria via phylogenetic methods].to differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrb gene sequences.200718067224
activation of prothrombin by asp, a serine protease released from aeromonas sobria.the effect of a serine protease (asp) secreted from aeromonas sobria on plasma coagulation was investigated. proteolytically active asp promoted human plasma coagulation in a dose-dependent manner. consistent with the preference for a factor xa-specific oligo-peptide substrate, asp produced enzymatic activity from human prothrombin but not from factors ix and x. asp cleaved prothrombin to produce enzymatically active 37 kda-fragment displaying the same molecular mass as alpha-thrombin. asp is th ...200718067862
structural characterization of the o-chain polysaccharide of aeromonas caviae atcc 15468 lipopolysaccharide.the o-chain polysaccharide produced by a mild acid degradation of aeromonas caviae atcc 15468 lipopolysaccharide was found to be composed of l-rhamnose, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose and phosphoglycerol. subsequent methylation and ce-esims analyses and 1d/2d nmr ((1)h, (13)c and (31)p) spectroscopy showed that the o-chain polysaccharide is a high-molecular-mass acidic branched polymer of tetrasaccharide repeating units with a phosphoglycerol substituent having th ...200818068695
occurrence of an sme-2-producing serratia marcescens isolate in canada. 200818083009
the effect of specific mhc class i and class ii combinations on resistance to furunculosis in atlantic salmon (salmo salar).the aim was to evaluate the performance of selected individual major histocompatibility complex (mhc) class i and class ii alleles and combinations of these on disease resistance against furunculosis. the material, consisting of 2,145 atlantic salmon (salmo salar) from seven families, contained four mhc class i (uba) alleles and two mhc class ii alpha (daa) alleles. the included alleles were previously shown to influence resistance towards furunculosis. the fish were challenged with the bacteriu ...200818086261
fluid secretion caused by aerolysin-like hemolysin of aeromonas sobria in the intestines is due to stimulation of production of prostaglandin e2 via cyclooxygenase 2 by intestinal cells.to clarify the mechanisms of diarrheal disease induced by aeromonas sobria, we examined whether prostaglandin e2 (pge2) was involved in the intestinal secretory action of a. sobria hemolysin by use of a mouse intestinal loop model. the amount of pge2 in jejunal fluid and the fluid accumulation ratio were directly related to the dose of hemolysin. the increase over time in the level of pge2 was similar to that of the accumulated fluid. in addition, hemolysin-induced fluid secretion and pge2 synth ...200818086811
antimicrobial activity of an endophytic xylaria sp.yx-28 and identification of its antimicrobial compound 7-amino-4-methylcoumarin.an endophytic xylaria sp., having broad antimicrobial activity, was isolated and characterized from ginkgo biloba l. from the culture extracts of this fungus, a bioactive compound p3 was isolated by bioactivity-guided fractionation and identified as 7-amino-4-methylcoumarin by nuclear magnetic resonance, infrared, and mass spectrometry spectral data. the compound showed strong antibacterial and antifungal activities in vitro against staphylococcus aureus [minimal inhibitory concentrations (mic) ...200818092158
temperate bacteriophage phio18p from an aeromonas media isolate: characterization and complete genome sequence.a group of 74 aeromonas isolates from surface water of three ponds in bielefeld, germany was screened for prophage induction after uv irradiation. the phage phio18p was induced from the aeromonas media isolate o18. phio18p belongs to the myoviridae phage family. the complete nucleotide sequence of the double stranded dna genome of bacteriophage phio18p consists of 33,985 bp. the genome has 5' protruding cohesive ends of 16 bases. on the phio18p genome 46 open reading frames (orfs) were identifie ...200818096197
antibiotic resistance of motile aeromonads in indoor catfish and eel farms in the southern part of the netherlands.the prevalence and degree of antibiotic resistance in catfish and eel farms in the southern part of the netherlands was examined using motile aeromonads as indicator bacteria. a total of 29 water samples were collected, originating from six catfish farms, one catfish hatchery and three eel farms, and were plated on an aeromonas-selective agar with and without antibiotics. from each plate, one colony was screened for presumptive motile aeromonads and tested for antibiotic susceptibility. the prev ...200818160266
comparison of the bactec blood culture system versus conventional methods for culture of normally sterile body fluids.this study compared the bactec blood culture system (becton dickinson diagnostic instrument systems, sparks, md) with conventional culture methods for recovery and time to detection of significant isolates from normally sterile body fluids. a total of 412 specimens were included in the study. half of the specimens were inoculated directly into the automated blood culture system. the remaining specimens were centrifuged at 3000 rpm for 10 min and were inoculated onto conventional media. clinicall ...200718165209
fulminant epiglottitis with evolution to necrotizing soft tissue infections and fasciitis due to aeromonas hydrophila. 200818165860
microbiologic study of soft contact lenses after laser subepithelial keratectomy for myopia.to evaluate the extent and agents of bacterial contamination of bandage disposable soft contact lenses after laser subepithelial keratectomy (lasek) and to correlate the findings with clinical data.200818180679
study on the biocompatibility of novel terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate).terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) (phbvhhx) containing 5.3 mol % 3-hydroxyvalerate (3-hv) and 10.2 mol % 3-hydroxyhexanoate was obtained via microbial synthesis using recombinant aeromonas hydrophila. for the first time in vitro biocompatibility of the terpolyester was evaluated in comparison with poly(l-lactic acid), poly (3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx). sc ...200818186048
a possible new cause of spa bath folliculitis: aeromonas hydrophila.a 34-year-old man presented with a pubic eruption of 4 weeks duration, affecting both himself and his partner. he had been treated unsuccessfully with intravenous and oral dicloxacillin. the eruption was a severely inflammatory folliculitis, with haemo-serous exudate and marked oedema. a clinical diagnosis of herpes simplex virus folliculitis was disproved when routine bacteriology isolated aeromonas hydrophila and multiple viral polymerase chain reaction studies were negative. histology demonst ...200818186847
aeromonas-acanthamoeba interaction and early shift to a viable but nonculturable state of aeromonas by acanthamoeba.to investigate the hypothesis that amoeba may comprise a significant environmental reservoir for aeromonas, acanthamoeba-aeromonas interaction experiments were performed.200818194253
chemical markers for the evaluation of raw material hygienic quality in egg products.the aim of this research was to study uracil and lactic and acetic acids as chemical markers for hygienic quality evaluation of raw material in liquid pasteurized egg products. uracil, absent in sound whole eggs, was formed in raw and pasteurized egg products as a consequence of high microbial contamination (>10(6) cfu/g) after a sufficient lag time, remaining stable at 4 degrees c but disappearing after 7 days of storage at 25 degrees c. both lactic and acetic acids, starting from initial value ...200818211016
contribution of type iv pili to the virulence of aeromonas salmonicida subsp. salmonicida in atlantic salmon (salmo salar l.).aeromonas salmonicida subsp. salmonicida, a bacterial pathogen of atlantic salmon, has no visible pili, yet its genome contains genes for three type iv pilus systems. one system, tap, is similar to the pseudomonas aeruginosa pil system, and a second, flp, resembles the actinobacillus actinomycetemcomitans flp pilus, while the third has homology to the mannose-sensitive hemagglutinin pilus of vibrio cholerae. the latter system is likely nonfunctional since eight genes, including the gene encoding ...200818212071
pathogenic vibrio, aeromonas and arcobacter spp. associated with copepods in the straits of messina (italy). 200818215401
[cellulitis caused by aeromonas hydrophila in an immunocompromised old woman]. 200718232124
the involvement of aeromonas salmonicida virulence factors in bacterial translocation across the rainbow trout, oncorhynchus mykiss (walbaum), intestine.the pathogenic bacterium aeromonas salmonicida is the causative agent of furunculosis, a lethal disease in salmonids. the mode of lateral transmission has not been conclusively defined, but a. salmonicida is able to translocate across the intestinal epithelium of salmonids, making the intestinal route a probable candidate. this study investigated some of the virulence mechanisms used by the bacteria to promote translocation. intestinal segments were placed in modified ussing chambers to investig ...200818234022
inactivation of host akt/protein kinase b signaling by bacterial pore-forming toxins.uropathogenic escherichia coli (upec) are the major cause of urinary tract infections (utis), and they have the capacity to induce the death and exfoliation of target uroepithelial cells. this process can be facilitated by the pore-forming toxin alpha-hemolysin (hlya), which is expressed and secreted by many upec isolates. here, we demonstrate that hlya can potently inhibit activation of akt (protein kinase b), a key regulator of host cell survival, inflammatory responses, proliferation, and met ...200818234841
application of antimicrobial ice for extending shelf life of fish.in this study, we investigated whether wild-thyme (thymus serpyllum) hydrosol had a preserving effect against spoilage of freshwater fish. sensorial characteristics, chemical freshness indicator contents, and microbial counts (total aerobes, psychrotrophics, enterobacteriaceae, fecal coliform bacteria, aeromonas spp., and pseudomonas spp.) of whole ungutted and gutted transcaucasian barb (capoeta capoeta capoeta guldenstaedt, 1772) stored on ice produced from wild-thyme hydrosol and tap water at ...200818236688
aeromonas-related diarrhoea in nasarawa, nigeria.two hundred and fifty volunteer patients attending 5 major hospitals in nasarawa town, nasarawa state, nigeria were involved in this study to determine the prevalence of aeromonas spp in persons with diarrhoea. the study population consisted of 133 males and 117 females between the ages of 0-70 years.200718240708
peptide hydrolysis by the binuclear zinc enzyme aminopeptidase from aeromonas proteolytica: a density functional theory study.aminopeptidase from aeromonas proteolytica (aap) is a binuclear zinc enzyme that catalyzes the cleavage of the n-terminal amino acid residue of peptides and proteins. in this study, we used density functional methods to investigate the reaction mechanism of this enzyme. a model of the active site was constructed on the basis of the x-ray crystal structure of the native enzyme, and a model dipeptide was used as a substrate. it was concluded that the hydroxide is capable of performing a nucleophil ...200818247603
comparison of the hemagglutination of formalinized american channel catfish (ictalurus punctatus) erythrocytes between formalinized and live aeromonas hydrophila isolated from the catfish rearing in kasumigaura lake in japan.formalinized aeromonas (a.) hydrophila agglutinated loosely with the formalinized american channel catfish erythrocytes (facce), while live a. hydrophila agglutinated tightly with the facce. there was a significant difference on the number of attaching bacterial cells to the facce (p<0.01) (n=40 erythrocytes) between formalinized and live a. hydrophila. the other bacteria such as salmonella (s.) typhimurium st-5, escherichia (e.) coli v-517 and staphylococcus (s.) hyicus atcc1249 used in this ex ...200818250583
the influence of selected antibiotics on aquatic microorganisms.this paper presents the influence of penicillin, erythromycin, oxytetracycline and streptomycin on aquatic microorganisms isolated from three reservoirs with varied extent of environmental pollution (sulej6w reservoir, zegrze reservoir and vistula river). the experiments were conducted in especially prepared microcosms (aerated 151 aquariums). from among the examined antibiotics streptomycin showed the longest activity in the water environment (27 days), followed by oxytetracycline (22 days). er ...200718254497
severe diarrhoea caused by aeromonas veronii biovar sobria in a patient with metastasised gist.this report describes the isolation of aeromonas veronii biovar sobria as the causative enteropathogen of diarrhoea in an oncological patient after failure of detection of other infectious agents. the case points out the severe and long course of the infection, the diagnostic dilemma, and the prompt recovery after antibiotic treatment.200718254499
gene cloning, expression, and characterization of a second beta-n-acetylglucosaminidase from the chitinolytic bacterium aeromonas hydrophila strain suwa-9.a gene coding for a second beta-n-acetylglucosaminidase (nagb) was isolated from the chitinolytic bacterium aeromonas hydrophila strain suwa-9. the nagb open reading frame encoded a polypeptide of 618 amino acid residues with a molecular mass of 68.8 kda. it did not contain a sequence characteristic of a signal peptide at the n-terminus. the deduced amino acid sequence showed high similarity to those of bacterial beta-n-acetylhexosaminidases classified into family 20 of glycosyl hydrolases. the ...200818256480
extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation.the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ...200818256966
unexpected occurrence of plasmid-mediated quinolone resistance determinants in environmental aeromonas spp.we searched for plasmid-mediated quinolone resistance determinants of the qnr type in several water samples collected at diverse locations from the seine river (paris, france). the qnrs2 genes were identified from aeromonas punctata subsp. punctata and a. media. the qnrs2 gene was located on incu-type plasmids in both isolates, which resulted in increased mic values of quinolones and fluoroquinolones, once they were transferred into escherichia coli. the qnrs2 gene identified in a. punctata was ...200818258115
characterization of vibrio cholerae isolated from the aquatic basins of the state of pernambuco, brazil.through a continuous bacteriological monitoring programme carried out by the health secretariat of the state of pernambuco, brazil, two isolates of vibrio cholerae o1 el tor ogawa were discovered in an endemic area in 2001, during a cholera inactive period, along with six v. cholerae non-o1/non-o139 strains and two aeromonas veronii biovar sobria strains showing an unusual characteristic of agglutination with o1 antiserum. between that time and 2005, eight other o1 isolates were found. the virul ...200818258274
biokinetic parameters and behavior of aeromonas hydrophila during anaerobic growth.the biokinetics of glucose metabolism were evaluated in aeromonas hydrophila during growth in an anaerobic biosystem. after approx 34 h growth, a. hydrophila metabolized 5,000 mg glucose l(-1) into the end-products ethanol, acetate, succinate and formate. the maximum growth rate, mu(m), half saturation coefficients, k(s), microbial yield coefficient, y, cell mass decay rate coefficient, k(d), and substrate inhibition coefficient, k(si) were 0.25 +/- 0.03 h(-1), 118 +/- 31 mg glucose l(-1), 0.12 ...200818265942
role of the aheabc efflux pump in aeromonas hydrophila intrinsic multidrug resistance.gene inactivation and complementation experiments showed that the tripartite aheabc efflux pump of aeromonas hydrophila extruded at least 13 substrates, including nine antibiotics. the use of phenylalanine-arginine-beta-naphthylamide (pabetan) revealed an additional system(s) contributing to intrinsic resistance. this is the first analysis of the role of multidrug efflux systems in aeromonas spp.200818268083
predictors of mortality in patients with necrotizing fasciitis.necrotizing fasciitis is an uncommon and life-threatening soft tissue infection with high mortality. though early aggressive surgical intervention is important for improving survival, the impact of mortality from different microorganisms remains uncertain. our study aims to identify the association of mortality and different microorganisms, and the positive and negative predictors of mortality in patients with necrotizing fasciitis.200818272096
recombinant production and characterization of the carbohydrate recognition domain from atlantic salmon c-type lectin receptor c (sclrc).the atlantic salmon c-type lectin receptor c (sclrc) locus encodes a potential oligomeric type ii receptor. c-type lectins recognize carbohydrates in a ca(2+)-dependent manner through structurally conserved, yet functionally diverse, c-type lectin-like domains (ctlds). many conserved amino acids in animal ctlds are present in sclrc, with the notable exception of an asparagine crucially involved in ca(2+)- and carbohydrate-binding, which is tyrosine in sclrc. sclrc also contains six cysteines tha ...200818272393
16s rrna gene sequences from bacteria associated with adult anopheles darlingi (diptera: culicidae) mosquitoes.the microbial flora associated with anopheles darlingi root (diptera: culicidae), a major neotropical malaria vector, was investigated for the development of a paratransgenesis-based approach to control malaria transmission in brazil. female mosquitoes were collected using human land catches and captured insects provided a bloodmeal. the controlled blood feeding resulted in increased detection of mosquito bacterial population because it was possible to retrieve bacterial dna from all blood-fed m ...200818283961
quorum sensing signals are produced by aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor.many pathogens control production of virulence factors by self-produced signals in a process called quorum sensing (qs). we demonstrate that acyl homoserine lactone (ahl) signals, which enable bacteria to express certain phenotypes in relation to cell density, are produced by a wide spectrum of aeromonas salmonicida strains. all 31 typical strains were ahl producers as were 21 of 26 atypical strains, but on a strain population basis, production of virulence factors such as protease, lipase, a-la ...200718286807
isolation, identification and application of novel bacterial consortium tj-1 for the decolourization of structurally different azo dyes.a novel bacterial consortium (tj-1), which could decolorize acid orange 7 (ao7) and manyother azo dyes, was developed. in tj-1 three bacterial strains were identified as aeromonas caviae, proteus mirabilis and rhodococcus globerulus by 16s rrna gene sequence analysis. ao7 decolorization was significantly higher with the use of consortium as compared to the use of individual strains, indicating complementary interactions among these strains. ao7 decolorization was observed under microaerophilic c ...200818289845
identification of siderophore biosynthesis genes essential for growth of aeromonas salmonicida under iron limitation conditions.aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, produces a catechol-type siderophore under iron-limiting conditions. in this study, the fur titration assay (furta) was used to identify a cluster of six genes, asbg, asbf, asbd, asbc, asbb, and asbi, encoding proteins similar to components of the siderophore biosynthetic machinery in other bacteria. reverse transcriptase pcr analyses showed that this cluster consists of four iron-regulated transcriptional u ...200818296539
multiresistant waterborne pathogens isolated from water reservoirs and cooling systems.to determine the incidence of multiple antibiotic-resistant strains of the emergent human pathogens legionella pneumophila, pseudomonas aeruginosa and mesophilic aeromonas species among those isolated from water reservoirs and industrial cooling systems.200818298535
culturable heterotrophic bacteria in seawater and mytilus galloprovincialis from a mediterranean area (northern ionian sea-italy).although mar piccolo of taranto (ionian sea, italy) is one of the most important mytilus galloprovincialis farming areas, data concerning the natural bacterial microbiota of these mussels and their surrounding environment are still scant. this study was carried out seasonally, throughout a year, to determine culturable heterotrophic bacteria both in the water and mussels samples collected at three sampling sites in the northern ionian sea: s. vito, lido gandoli and lido silvana. culturable heter ...200918301997
molecular analysis of three aeromonas hydrophila ah-3 (serotype o34) lipopolysaccharide core biosynthesis gene clusters.by the isolation of three different aeromonas hydrophila strain ah-3 (serotype o34) mutants with an altered lipopolysaccharide (lps) migration in gels, three genomic regions encompassing lps core biosynthesis genes were identified and characterized. when possible, mutants were constructed using each gene from the three regions, containing seven, four, and two genes (regions 1 to 3, respectively). the mutant lps core structures were elucidated by using mass spectrometry, methylation analysis, and ...200818310345
vibrio cholerae and aeromonas: do they share a mutual host?species of the genus aeromonas are native inhabitants of aquatic environments and have recently been considered as an emergent human pathogen. it is estimated that aeromonads cause up to 13% of reported gastroenteritis cases in the united states. although the autochthonous existence of aeromonas in the aquatic environment has been established, its natural reservoir is as yet unknown. chironomids are closely related to mosquitoes except they do not bite and they are the most widely distributed in ...200818317460
functionality of a bacillus cereus biological agent in response to physiological variables encountered in aquaculture.the potential of a bacillus cereus isolate (nrrl 100132) as a biological agent for aquaculture has been demonstrated in vitro and in vivo. the functionality of this isolate across a range of physiological conditions, including salinity, ph and temperature, based on rearing of high-value ornamental cyprinus carpio, was investigated. temperature had a significant influence on germination, specific growth rate and increase in cell number of b. cereus in shake-flask cultures, whilst salinity and ph ...200818317748
survival of freeze-dried bacteria.the aim of this study was to investigate the survival of freeze-dried bacterial species stored at the international patent organism depository (ipod) and to elucidate the characteristics affecting survival. bacterial strains were freeze-dried, sealed in ampoules under a vacuum (<1 pa), and stored in the dark at 5 degrees c. the survival of a variety of species following storage for up to 20 years was analyzed. the survival of freeze-dried species was analyzed in terms of two stages, freeze-dryin ...200818323678
[aeromonas spp. infections: retrospective study in nîmes university hospital, 1997-2004].retrospective study of patients presenting aeromonas spp. infections admitted to nîmes hospital from january 1997 to december 2004.200818329823
molecular cloning of liver angiotensinogen gene in ayu (plecoglossus altivelis) and mrna expression changes upon aeromonas hydrophila infection.angiotensinogen (agt) is thought to be an acute phase protein, because in mammals its plasma concentrations increase during inflammation induced by lipopolysaccharides (lps). a full-length cdna clone of the agt gene, 1828bp in size, was isolated from the fish ayu (plecoglossus altivelis). its deduced amino acid sequence of 462 residues had 57.7-68.6%, 30.2% and 23.3-27.4% identity to those sequenced from other fish, chicken and mammals, respectively. ayu-agt is most closely related to that of si ...200818337123
role of virulence plasmid of aeromonas hydrophila in the pathogenesis of ulcerative disease syndrome in clarias batrachus.pathogenic aeromonas hydrophila (strain vb21), a multiple-drug resistance strain contains a plasmid of about 21 kb. after curing of plasmid, the isolates became sensitive to antimicrobials, to which they were earlier resistant. the cured bacteria exhibited significant alterations in their surface structure, growth profile and virulence properties, and failed to cause ulcerative disease syndrome (uds) when injected into the indian catfish clarias batrachus. routine biochemical studies revealed th ...200718341217
cold shock exoribonuclease r (vacb) is involved in aeromonas hydrophila pathogenesis.in this study, we cloned and sequenced a virulence-associated gene (vacb) from a clinical isolate ssu of aeromonas hydrophila. we identified this gene based on our recently annotated genome sequence of the environmental isolate atcc 7966(t) of a. hydrophila and the vacb gene of shigella flexneri. the a. hydrophila vacb protein contained 798 amino acid residues, had a molecular mass of 90.5 kda, and exhibited an exoribonuclease (rnase r) activity. the rnase r of a. hydrophila was a cold-shock pro ...200818344363
evaluation of reca sequencing for the classification of aeromonas strains at the genotype level.to evaluate the usefulness of partial reca sequences for the identification of aeromonas strains at the genotype level.200818346137
development and application of real-time pcr assays for quantifying total and aerolysin gene-containing aeromonas in source, intermediate, and finished drinking water.aeromonas spp., opportunistic pathogens, are listed as a microbiological contaminant on the environmental protection agency's (epa) drinking water contaminant candidate list. culture-based methods for identification and quantification of aeromonas in drinking water are time-consuming and often fail to differentiate pathogenic species from nonpathogenic ones. this study reports successful development and applications of two real-time pcr assays, based on 16s rrna gene sequences and a virulence ge ...200818351092
identification of bacteria from the normal flora of perch, perca fluviatilis l., and evaluation of their inhibitory potential towards aeromonas species.pathogenic aeromonas sobria has been identified as a causative agent of ulcerative disease in farmed european perch, perca fluviatilis l. to study the effect of the normal intestinal bacterial flora of perch against a. sobria, we sampled 193 bacterial isolates from the perch digestive tract. the isolates were identified by sequence analysis of the 16s rrna gene and their inhibitory potential against a. sobria was evaluated in vitro. nineteen of the strains isolated showed inhibition and were als ...200818355180
chemotaxis towards, adhesion to, and growth in carp gut mucus of two aeromonas hydrophila strains with different pathogenicity for common carp, cyprinus carpio l.characteristics that promote bacterial colonization of the intestinal mucosal surface were examined in two strains of the common fish pathogen aeromonas hydrophila, with different pathogenicity. the characteristics examined were chemotactic activity towards mucus, bacterial adherence to mucus and growth in mucus. intestinal gut mucus of healthy common carp was used. the results indicate that chemotaxis is not necessary for a bacterium to become pathogenic, but it may be a necessary parameter for ...200818355183
degradation of linear alkylbenzene sulfonate by a bacterial consortium isolated from the aquatic environment of argentina.to isolate and identify linear alkylbenzene sulfonate (las)-degrading bacteria from río de la plata and adjacent waters, and to assay their degradation capability as a consortium and as single organisms.200818355233
effect of packaging atmosphere on the microbial attributes of pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c.effect of packaging atmosphere (air and under different modified atmospheres (mas), 40% co2/60% o2, 50%/50% o2, 60% co2/40% o2, 70% co2/30% o2 and 40% co2/30% o2/30% n2) on the microbial and biochemical attributes of fresh pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c was investigated. trimethylamine nitrogen (tma-n) and thiobarbituric acid (tba) values remained lower than the proposed acceptability limits throughout the storage period. results demonstrated that storage of pearls ...200818355677
identification of the first vim metallo-beta-lactamase-producing multiresistant aeromonas hydrophila strain.a vim metallo-beta-lactamase-producing aeromonas hydrophila strain carrying an integron-borne bla(vim-4) gene was isolated from a cirrhotic patient's fecal sample in a budapest hospital. the variable region of this integron is identical with that of a previously characterized integron from pseudomonas aeruginosa clinical isolates in pécs in southern hungary.200818367570
transferable, multiple antibiotic and mercury resistance in atlantic canadian isolates of aeromonas salmonicida subsp. salmonicida is associated with carriage of an inca/c plasmid similar to the salmonella enterica plasmid psn254.the aim of this study was to examine the molecular basis for multiple antibiotic and mercury resistance in canadian isolates of aeromonas salmonicida subsp. salmonicida.200818375380
[microbiological aspects of pool water].it is well known that there is an elevated risk of diffusion of pathogenic micro-organisms in swimming pools. preventive measures aimed at protecting the health of swimmers are complex because of the variety of micro-organisms involved, the different ways in which these can be transmitted and the involvement of both aquatic and environmental factors. in industrialised countries, the circulation of many pathogens which were common in the past has progressively decreased in recent years; in contra ...200818379609
necrotizing skin and soft-tissue infections associated with septicemia: 7 cases report and review.the authors report seven cases of necrotizing skin and soft-tissue infections, with clinical presenting as hemorrhagic bullae, gangrenous cellulitis or necrotizing fasciitis, in association with septicemia, between january 2003 and january 2007 in hat yai hospital. six were male and the majority of the lesions, six cases, occurred in the lower extremities. the average age of the patients was 50.0 +/- 11.019 years old. all patients presented with watery diarrhea, severe abdominal pain, high fever ...200818386555
prevalence of bacterial pathogens and their anti-microbial resistance in tilapia and their pond water in trinidad.in trinidad, tilapia (oreonchromis spp.) is one of the most important fresh water food fish and the number of farms has been increasing annually. a study was conducted in the local tilapia industry to determine the microbial quality of pond water, prevalence of bacterial pathogens and their anti-microbial resistance using the disk diffusion method. seventy-five apparently healthy fish and 15 pond water samples from three of the four commercial tilapia fish farms in the country were processed. th ...200818387142
pathogenic potential of a collagenase gene from aeromonas veronii.the role of collagenase as a mechanism of bacterial pathogenicity in some pathogenic bacteria has been reported. the information on the role of collagenase in aeromonas spp. pathogenesis is scant. in the present study, a mutant aeromonas veronii ry001 that is deficient in the putative collagenase gene acg was constructed and compared with the wild-type strain for virulence factors. bacterial cells and cell-free extracellular products of the mutant had significantly less collagenolytic activity, ...200818388966
cloning, expression, and transglycosylation reaction of paenibacillus sp. strain w-61 xylanase 1.a xylanase gene, xyn1, which encodes paenibacillus sp. strain w-61 xylanase 1 (xyn1), was cloned in escherichia coli. xyn1 encodes 211 amino acid residues, including 28 amino acid residues of a signal peptide. the deduced amino acid sequence of the mature xyn1 showed 95.7%, 84.0%, and 83.7% identity to family 11 xylanases of aeromonas caviae me-1, paenibacillus sp., and bacillus stearothermophilus respectively. the physico-chemical properties of recombinant xyn1 were very similar to those of int ...200818391461
viscoelastic properties of an exopolysaccharide: aeromonas gum, produced by aeromonas nichidenii 5797.the viscoelastic properties of aeromonas (a) gum in water were investigated by using the rheometric scientific ares controlled strain rheometer. an intrinsic viscosity of 8336 ml/g was obtained according to the fuoss-straus equation. the effect of salt concentration on intrinsic viscosity revealed that the a gum exists as semiflexible chain. typical shear-thinning (pseudoplastic) behavior was observed at concentrations higher than 0.52%. the zero shear viscosity (eta(0)) increased with increasin ...200718401077
prevalence, pathogenesis, antibiotic susceptibility profiles, and in-vitro activity of selected medicinal plants against aeromonas isolates from stool samples of patients in the venda region of south africa.the prevalence, pathogenic indices, such as haemolytic and haemagglutinating activities, antibiograms, and in-vitro activities of local medicinal plants against aeromonas isolates in vhembe district of limpopo province, south africa, were studied using standard microbiological methods. in total, 309 diarrhoeic stool samples were collected from patients attending five health centres in the region during december 2004-may 2005. aeromonas species were identified using the api 20e system. the haemag ...200718402186
antimicrobial activity of tissue and associated bacteria from benthic sea anemone stichodactyla haddoni against microbial pathogens.associated bacteria from stichodactyla haddoni are found maximum in tentacle tissues than the body tissue. there are eight associated bacterial species viz., alcaligenes sp, corynebacterium sp, aeromonas sp, sporosarcina sp, renibacterium sp, camobacterium sp1, camobacterium sp2 and salinococcus sp were recorded. the culture extracts from the associated bacterial species showed sensitivity against human bacterial and fungalpathogens. however, the hexane tissue extract of sea anemone showed maxim ...200718405113
cloning, binding properties, and tissue localization of rainbow trout (oncorhynchus mykiss) ladderlectin.the present paper describes the primary structure, expression and immunohistochemical localization of rainbow trout ladderlectin (rtll), a multimeric serum lectin that binds sepharose and lps of aeromonas salmonicida. two rainbow trout cdnas (504 and 546bp) and a genomic sequence (2kb) were amplified using ladderlectin-specific primers. the sequences were identified as group vii mannose-binding c-type lectins from predicted amino acid sequences and showed highest identity with the atlantic salmo ...200818407519
the aeromonas hydrophila wb*o34 gene cluster: genetics and temperature regulation.the aeromonas hydrophila wb*(o34) gene cluster of strain ah-3 (serotype o34) was cloned and sequenced. this cluster contains genes necessary for the production of o34-antigen lipopolysaccharide (lps) in a. hydrophila. we determined, using either mutation or sequence homology, roles for the majority of genes in the cluster by using the chemical o34-antigen lps structure obtained for strain ah-3. the o34-antigen lps export system has been shown to be a wzy-dependent pathway typical of heteropolysa ...200818408022
complete sequence of the flor-carrying multiresistance plasmid pab5s9 from freshwater aeromonas bestiarum.a multiresistant aeromonas bestiarum strain, shown to be persistent and spreading in a freshwater stream, was investigated for the presence, location and organization of antimicrobial resistance genes.200818413319
aeromonas hydrophila bacteraemia and portal pyaemia.the aeromonas species uncommonly cause disease in humans. we report portal pyaemia secondary to aeromonas hydrophila bacteraemia occurring in a 71-year-old chinese man with no history of hepatobiliary disease or malignancy. he presented with fever, rigors and abdominal bloating for four days and was subsequently found to have aeromonas hydrophila bacteraemia, portal vein thrombosis and a psoas abscess. he was treated with ciprofloxacin and had a good recovery. aeromonas hydrophila infection is a ...200818418529
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