| detection of sexually transmitted pathogens in patients with chronic prostatitis/chronic pelvic pain: a prospective clinical study. | in <10% of patients with prostatitis syndrome, a causative uropathogenic organism can be detected. it has been shown that certain organisms that cause sexually transmitted infections can also cause chronic bacterial prostatitis, which can be hard to diagnose and treat appropriately because prostatic samples obtained by prostatic massage are not routinely tested to detect them. we conducted a clinical study to determine the prevalence of chlamydia, mycoplasma, and trichomonas infection in 254 pat ... | 2017 | 28120647 |
| clinical performance of the solana® point-of-care trichomonas assay from clinician-collected vaginal swabs and urine specimens from symptomatic and asymptomatic women. | solana® (quidel) is a new rapid (<40 min.) point-of-care (poc) test for qualitative detection of trichomonas vaginalis (tv) dna. the assay has two steps: 1) specimen preparation, and 2) amplification and detection using isothermal helicase-dependent amplification (hda). the objective was to demonstrate the performance of solana for vaginal swabs and female urines based on comparison to wet mount and tv culture. performance was also compared to the aptima-tv assay. | 2017 | 28092466 |
| multiple hpv infections in female sex workers in western kenya: implications for prophylactic vaccines within this sub population. | whilst the imputed role of high risk (hr) hpv infection in the development of cervical lesions and cancer has been established, the high number of hpv genotypes that female sex workers (fsw) harbour warrants that the synergistic effects of potential hr (phr) and hr hpv genotypes be elucidated to assess the potential impact of prophylactic vaccines. this population in kenya also harbours a number of other vaginal infections and stis, including bacterial vaginosis (bv), trichomonas vaginalis (tv) ... | 2017 | 28070215 |
| epigenetics regulates transcription and pathogenesis in the parasite trichomonas vaginalis. | trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract. infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. different t. vaginalis strains vary greatly in their adherence and cytolytic capacities. these phenotypic differences might be attributed to differentially expressed genes as a consequence of extra-genetic variation, such as epigenetic modifications. in this study, we explored the role ... | 2017 | 28054438 |
| a nonobstructive azoospermic patient with trichomonas vaginalis infection in testes. | | 2017 | 28051042 |
| a study on the prevalence of genital trichomoniasis among female outpatients attending sexually transmitted infection clinic in a tertiary care hospital. | women with high-risk sexual behavior accounts for more than half of the sexually transmitted infection (sti) clinic attendees. the prevalence of trichomoniasis is as low as 5% in the general population to as high as 60% in high-risk population. this infection can pave the way to the acquisition of human immunodeficiency virus and other stis, vice versa and is even associated with cancer. | 2017 | 28042211 |
| trichomonas vaginalis treated with boric acid in a metronidazole allergic female. | | 2017 | 27984554 |
| ginger (zingiber officinale) induces apoptosis in trichomonas vaginalis in vitro. | trichomoniasis is the most common sexually transmitted protozoan diseases in the worldwide. metronidazole is the choice drug for trichomoniasis treatment, however, metronidazole resistant trichomonas vaginalis (t.vaginalis) has been reported. natural products are the source of most new drugs, and zingiber officinale (ginger) is widely used ingredient in the traditional medicine. | 2016 | 27981254 |
| prevalence and associated factors of genital and sexually transmitted infections in married women of iran. | this study aimed to determine the prevalence of genital and sexually transmitted infections and its related factors in married women in iran. | 2016 | 27974960 |
| the caatinga endemic manilkara rufula possesses remarkable activity against trichomonas vaginalis and tritrichomonas foetus. | tritrichomonas foetus infects the bovine urogenital tract, causing bovine trichomoniasis. significant economic losses may occur due to infertility and abortion among cattle. trichomonas vaginalis is the causative agent of trichomoniasis; the most common but overlooked non-viral sexually transmitted disease worldwide. human and bovine trichomoniasis present treatment restrictions and efforts to identify new alternatives are essential. the present study evaluated the anti-trichomonads activities o ... | 2017 | 27956086 |
| epidemiology of trichomoniasis in south korea and increasing trend in incidence, health insurance review and assessment 2009-2014. | trichomoniasis, which is caused by trichomonas vaginalis, is one of the most common non-viral sexually transmitted infections; however, limited population-based data are available that describe patterns and trends of the disease. we summarized insurance claims of trichomoniasis cases reported during 2009-2014 to south korea health insurance review and assessment service. the average annual incidence in south korea was 276.8 persons per 100,000 population, and a substantial sex-associated variati ... | 2016 | 27936227 |
| antiprotozoal effects of the tomato tetrasaccharide glycoalkaloid tomatine and the aglycone tomatidine on mucosal trichomonads. | the present study investigated the inhibitory effects of the commercial tetrasaccharide tomato glycoalkaloid tomatine and the aglycone tomatidine on three mucosal pathogenic protozoa that are reported to infect humans, cattle, and cats, respectively: trichomonas vaginalis strain g3, tritrichomonas foetus strain d1, and tritrichomonas foetus strain c1. a preliminary screen showed that tomatine at 100 μm concentration completely inhibited the growth of all three trichomonads. in contrast, the inhi ... | 2016 | 27934291 |
| in situ forming pluronic® f127/chitosan hydrogel limits metronidazole transmucosal absorption. | the objective of this work is to design topically-applied thermosensitive and mucoadhesive hydrogel containing metronidazole (mtz) for the treatment of trichomonas vaginalis infections. hydrogel composed of pluronic® f127 (20wt%), chitosan (1wt%) and metronidazole mtz (0.7wt%) mixture showed its ability to decrease by a factor 4 mtz flux and apparent permeability absorption through vaginal mucosa. the impact of hydrogel on transmucosal penetration of mtz was evaluated ex vivo on excised porcine ... | 2017 | 27890510 |
| trichomoniasis in older individuals: a preliminary report from iran. | infection with trichomonas vaginalis is one of the most common sexually transmitted diseases (stds) in humans. the prevalence of infection in iran has been reported 0.009-8 % depending on deferent socio-cultural conditions. this study aimed to determine the frequency of t. vaginalis according to age in patients referred to clinics, hospitals and medical diagnostic laboratories in karaj city, iran. for this purpose, fifty positive samples were collected from july 2012 to june 2014 from clinics, m ... | 2016 | 27876991 |
| adenosine, but not guanosine, protects vaginal epithelial cells from trichomonas vaginalis cytotoxicity. | trichomonas vaginalis causes the most common non-viral sexually transmitted disease worldwide. the cytoadherence and cytotoxicity upon the vaginal epithelial cells are crucial for the infection. extracellular nucleotides are released during cell damage and, along with their nucleosides, can activate purinoceptors. the opposing effects of nucleotides versus nucleosides are regulated by ectonucleotidases. herein we evaluated the hemolysis and cytolysis induced by t. vaginalis, as well as the extra ... | 2017 | 27871906 |
| auranofin inactivates trichomonas vaginalis thioredoxin reductase and is effective against trichomonads in vitro and in vivo. | trichomoniasis, caused by the protozoan parasite trichomonas vaginalis, is the most common, non-viral, sexually transmitted infection in the world, but only two closely related nitro drugs are approved for its treatment. new antimicrobials against trichomoniasis remain an urgent need. several organic gold compounds were tested for activity against t. vaginalis thioredoxin reductase (trxr) in cell-free systems as well as for activity against different trichomonads in vitro and in a murine infecti ... | 2016 | 27839893 |
| comparative protein structure modeling using modeller. | comparative protein structure modeling predicts the three-dimensional structure of a given protein sequence (target) based primarily on its alignment to one or more proteins of known structure (templates). the prediction process consists of fold assignment, target-template alignment, model building, and model evaluation. this unit describes how to calculate comparative models using the program modeller and how to use the modbase database of such models, and discusses all four steps of comparativ ... | 2016 | 27801516 |
| benefits of cervical cancer screening by liquid-based cytology as part of routine antenatal assessment. | to determine the prevalence of abnormal cervical cytology, as diagnosed using a liquid-based cytology technique, in pregnant women attending the antenatal care (anc) clinic at siriraj hospital. | 2016 | 27797261 |
| snap23-dependent surface translocation of leukotriene b4 (ltb4) receptor 1 is essential for nox2-mediated exocytotic degranulation in human mast cells induced by trichomonas vaginalis-secreted ltb4. | trichomonas vaginalis is a sexually transmitted parasite that causes vaginitis in women and itself secretes lipid mediator leukotriene b4 (ltb4). mast cells are important effector cells of tissue inflammation during infection with parasites. membrane-bridging snare (soluble n-ethylmaleimide-sensitive factor attachment protein receptor) complexes are critical for fusion during exocytosis. although t. vaginalis-derived secretory products (tvsp) have been shown to induce exocytosis in mast cells, i ... | 2017 | 27795355 |
| a pilot study on trichomonas vaginalis in women with recurrent urinary tract infections. | trichomoniasis and recurrent urinary tract infections (utis) shared similar risk factors, age distribution and overlapping symptoms. the aim of this study was to determine the prevalence of trichomonas vaginalis (tv) in women with recurrent utis, attending a urology clinic in a medical center, in order to inform screening and treatment policies. | 2016 | 27793272 |
| trichomoniasis immunity and the involvement of the purinergic signaling. | innate and adaptive immunity play a significant role in trichomoniasis, the most common non-viral sexually transmitted disease worldwide. in the urogenital tract, innate immunity is accomplished by a defense physical barrier constituted by epithelial cells, mucus, and acidic ph. during infection, immune cells, antimicrobial peptides, cytokines, chemokines, and adaptive immunity evolve in the reproductive tract, and a proinflammatory response is generated to eliminate the invading extracellular p ... | 2016 | 27793265 |
| signalling pathways associated with il-6 production and epithelial-mesenchymal transition induction in prostate epithelial cells stimulated with trichomonas vaginalis. | trichomonas vaginalis (tv) has been found in patient tissue of benign prostatic hyperplasia (bph), and suggested to cause chronic prostatitis. il-6 is known as one of the important factors of chronic inflammation in prostate cancer. patients with chronic prostatitis/chronic pelvic pain syndrome (cp/cpps) had higher levels of il-6 in seminal plasma. furthermore, inflammatory conditions induced by pathogen infections have been shown to promote epithelial-mesenchymal transition (emt). here, we inve ... | 2016 | 27543848 |
| trichomonas vaginalis infection in nigerian pregnant women and risk factors associated with sexually transmitted infections. | trichomoniasis poses a public health threat to pregnant women and neonatal health. this study evaluated trichomonas vaginalis and other common sexually transmitted infections (stis) status in pregnant women, and risk factors associated with them. the study was cross-sectional and descriptive and a total of 198 pregnant women were recruited for t. vaginalis screening by microscopic examination. questionnaires were also administered to 108 pregnant women to access information related to socio-demo ... | 2016 | 26438604 |
| multilocus sequence typing of trichomonas vaginalis clinical samples from amsterdam, the netherlands. | in this cross-sectional epidemiological study we aimed to identify molecular profiles for trichomonas vaginalis and to determine how these molecular profiles were related to patient demographic and clinical characteristics. | 2016 | 27737887 |
| challenges and persistent questions in treatment of trichomoniasis. | trichomoniasis is a sexually transmitted disease (std) caused by infection with the protozoan parasite trichomonas vaginalis. it is considered the most prevalent non-viral sexually transmitted disease worldwide. recently, the infection has been associated with adverse outcomes of pregnancy and increased risks of hiv acquisition and transmission, besides the association with cervical and prostate cancers. the consequences of trichomoniasis are likely much greater than previously recognized, both ... | 2016 | 27697044 |
| trichomonas vaginalis infection is uncommon in the british general population: implications for clinical testing and public health screening. | variable use of new molecular assays, asymptomatic infections and a lack of population data mean that the population burden of trichomonas vaginalis is uncertain. we investigated the age-specific prevalence of t. vaginalis within the sexually active british general population to inform testing strategies. | 2016 | 27686884 |
| anti-trichomonas vaginalis activity of betulinic acid derivatives. | caused by trichomonas vaginalis, trichomoniasis is the most common non-viral std worldwide. currently, metronidazole and tinidazole are the only drugs approved for treatment of the condition. however, problems such as metronidazole-resistant t. vaginalis isolates and allergic reactions have been reported. based on data previously published by our group, structural changes in betulinic acid (1) were performed, generating three new compounds that were tested for in vitro anti-t.vaginalis activity ... | 2016 | 27685791 |
| trichomonas vaginalis prevalence and risk factors for women in southern brazil. | trichomonas vaginalis infections have been associated with other diseases so that epidemiological studies of the parasite are important and help to prevent the spread of the disease. this study aimed to determine the prevalence of t. vaginalis in female patients of 19 counties in southwestern rio grande do sul, brazil. for diagnosis, was used direct examination, followed by applying a socio-epidemiological questionnaire. we analyzed 300 women and 9% were infected by trichomonas vaginalis. the hi ... | 2016 | 27680166 |
| technical note: preservation of trichomonas vaginalis viability in urine for laboratorial diagnosis by the wet mount examination. | this study compared preservative solutions at different temperatures aiming to improve the wet mount for trichomoniasis diagnosis. the glucose-saline ph6.0 solution preserved the trophozoites up to 6h. the urine samples preservation is crucial for diagnosis and we suggest this solution as part of the clinical laboratorial routine. | 2016 | 27666653 |
| 2-methyl-4/5-nitroimidazole derivatives potentiated against sexually transmitted trichomonas: design, synthesis, biology and 3d-qsar study. | trichomoniasis is the most prevalent, non-viral sexually transmitted diseases (std) caused by amitochondriate protozoan trichomonas vaginalis. increased resistance of t. vaginalis to the marketed drug metronidazole necessitates the development of newer chemical entities. a library of sixty 2-methyl-4/5-nitroimidazole derivatives was synthesized via nucleophilic ring opening reaction of epoxide and the efficacies against drug-susceptible and -resistant trichomonas vaginalis were evaluated. all th ... | 2016 | 27643640 |
| diagnosis of trichomoniasis in men by urine cytology. | trichomonas vaginalis is a rare finding in urine cytology specimens, especially those from men; only 2 case reports have been described in the literature. the authors of the current report sought to determine the incidence and clinical significance of this finding in urine cytology in males. | 2017 | 27636204 |
| recombinant trichomonas vaginalis eif-5a protein expressed from a eukaryotic system binds specifically to mammalian and putative trichomonal eif-5a response elements (eres). | trichomonas vaginalis eif-5a-like protein (tveif-5a) belongs to the highly conserved eif-5a family of proteins that contains a unique polyamine-derived amino acid, hypusine. recently, we determined that the polyamine putrescine is required for tveif-5a mrna stability, and it is necessary for stability and maturation of the tveif-5a protein. eukaryotic eif-5a is known to be involved in mrna turnover and is capable of sequence-specific rna binding to eif-5a response elements (eres). these ere sequ ... | 2016 | 27620329 |
| sending a message: extracellular vesicles of pathogenic protozoan parasites. | parasitic unicellular eukaryotes use extracellular vesicles (evs) as vehicles for intercellular communication and host manipulation. by using various mechanisms to generate evs and by transferring a wide range of molecules through evs, pathogenic protozoans are able to establish infective niches, modulate the immune system of the host and cause disease. in addition to effects on the host, evs are able to transfer virulence factors, drug-resistance genes and differentiation factors between parasi ... | 2016 | 27615028 |
| trichomonas vaginalis: a possible foe to prostate cancer. | prostate cancer (pca) is the most common malignancy in men in usa, and the role of trichomonas vaginalis (t. vag) in the development of pca is still controversial. clonogenic assay, pcna staining, tunel staining and caspase-3 activity assay were used to investigate the in vitro role of t. vag in human prostate cancer. we further investigated the possible molecular mechanisms using rt-pcr and immunohistochemical staining. culture supernatant of t. vag inhibits growth of pc-3 prostate cancer cells ... | 2016 | 27613161 |
| effect of nigella sativa alcoholic extract and oil, as well as phaseolus vulgaris (kidney bean) lectin on the ultrastructure of trichomonas vaginalis trophozoites. | trichomonas vaginalis is a parasitic protozoan that is the aetiological agent of trichomoniasis, the most common non-viral sexually transmitted disease worldwide. currently, the compound of choice for the treatment of t. vaginalis infections is metronidazole, however, it has many side effects and an increase in metronidazole-resistant trichomoniasis has been observed. medicinal plants could be a source of new antiprotozoal drugs with high activity, low toxicity and lower price. the present work ... | 2016 | 27605771 |
| a calcium sensor - protein kinase signaling module diversified in plants and is retained in all lineages of bikonta species. | calcium (ca(2+)) signaling is a universal mechanism of signal transduction and involves ca(2+) signal formation and decoding of information by ca(2+) binding proteins. calcineurin b-like proteins (cbls), which upon ca(2+) binding activate cbl-interacting protein kinases (cipks) regulate a multitude of physiological processes in plants. here, we combine phylogenomics and functional analyses to investigate the occurrence and structural conservation of cbl and cipk proteins in 26 species representi ... | 2016 | 27538881 |
| male partner circumcision associated with lower trichomonas vaginalis incidence among pregnant and postpartum kenyan women: a prospective cohort study. | trichomonas vaginalis is the world's most common curable sti and has implications for reproductive health in women. we determined incidence and correlates of t. vaginalis in an hiv-uninfected peripartum cohort. | 2017 | 27519258 |
| corrigendum to "α-actinin tvactn3 of trichomonas vaginalis is an rna-binding protein that could participate in its posttranscriptional iron regulatory mechanism". | [this corrects the article doi: 10.1155/2014/424767.]. | 2017 | 27517042 |
| purification and identification of amylases released by the human pathogen trichomonas vaginalis that are active towards glycogen. | the parasitic protist trichomonas vaginalis is the causative agent of the sexually transmitted infection trichomoniasis. in the laboratory, t. vaginalis is typically cultured in a serum-containing medium with maltose or glucose as the carbon source. the nature of the carbohydrates used by the organism in the environment of its host is unclear. however, the vagina contains substantial amounts of glycogen, which is believed to provide a growth substrate for the vaginal microbiota. we have shown pr ... | 2016 | 27506885 |
| self-testing for trichomonas vaginalis at home using a point-of-care test by women who request kits via the internet. | we offered a point-of-care test for trichomonas vaginalis to women via the internet to determine if it was acceptable to women to perform the test at home. most of the 102 participants felt that it was easy to collect the specimen, follow the instructions, and read and interpret the results for the trichomonas self-testing assay. | 2016 | 27491592 |
| the glycolytic enzyme triosephosphate isomerase of trichomonas vaginalis is a surface-associated protein induced by glucose that functions as a laminin- and fibronectin-binding protein. | triosephosphate isomerase of trichomonas vaginalis (tvtim) is a 27-kda cytoplasmic protein encoded by two genes, tvtim1 and tvtim2, that participates in glucose metabolism. tvtim is also localized to the parasite surface. thus, the goal of this study was to identify the novel functions of the surface-associated tvtim in t. vaginalis and to assess the effect of glucose as an environmental factor that regulates its expression and localization. reverse transcription-pcr (rt-pcr) showed that the tvt ... | 2016 | 27481251 |
| molecular sensitivity threshold of wet mount and an immunochromatographic assay evaluated by quantitative real-time pcr for diagnosis of trichomonas vaginalis infection in a low-risk population of childbearing women. | vaginal trichomoniasis is a sexually transmitted infection caused by trichomonas vaginalis, a flagellated protozoan. diagnosis of t. vaginalis infection is mainly performed by wet mount microscopy, with a sensitivity ranging from 38% to 82%, compared to culture, still considered the gold standard. commercial immunochromatographic tests for monoclonal-antibody-based detection have been introduced as alternative methods for diagnosis of t. vaginalis infection and have been reported in some studies ... | 2016 | 27367320 |
| morphological changes of trichomonas vaginalis treated by ornidazole: an in-vitro study. | nitroimidazoles, which are drugs that are used to effectively treat trichomonas vaginalis, alter the structure of the t. vaginalis cell membrane, penetrate into its cytoplasm and nucleus and block cellular metabolism. in this study, we observed the morphological changes that occurred in t. vaginalis during in vitro exposure to 1.3 μg/ml of ornidazole at various time intervals ranging from 10 minutes to 10 hours. | 2016 | 27349003 |
| aggregation kinetic dataset to determine the stability of the purified and refolded recombinant pptvcp4 protein of trichomonas vaginalis. | the recombinant pptvcp4 (pptvcp4r) protein, a specific inhibitor of the proteolytic activity and virulence properties of trichomonas vaginalis, depending on cathepsin l-like cysteine proteinases (cps) (http:dx.doi.org/ 10.1016/j.biocel.2014.12.001[1], http:dx.doi.org/ 10.1016/j.micinf.2013.09.002[2], http:dx.doi.org/ 10.1155/2015/946787[3]) was stable in the elution buffer up to two months at 4 °c. however, it was prone to aggregate in pbs (functional assay buffer) [1]. therefore, before functio ... | 2016 | 27331109 |
| proliferation of prostate stromal cell induced by benign prostatic hyperplasia epithelial cell stimulated with trichomonas vaginalis via crosstalk with mast cell. | chronic inflammation has a role in the pathogenesis of benign prostatic hyperplasia (bph) and prostate cancer. mast cells have been detected in chronic inflammatory infiltrate of the prostate, and it is possible that the interaction between prostate epithelial cells and trichomonas vaginalis influences the activity of mast cells in the prostate stroma. activated mast cells might influence the biological functions of nearby tissues and cells. in this study, we investigated whether mast cells reac ... | 2016 | 27325623 |
| comparative protein structure modeling using modeller. | comparative protein structure modeling predicts the three-dimensional structure of a given protein sequence (target) based primarily on its alignment to one or more proteins of known structure (templates). the prediction process consists of fold assignment, target-template alignment, model building, and model evaluation. this unit describes how to calculate comparative models using the program modeller and how to use the modbase database of such models, and discusses all four steps of comparativ ... | 2016 | 27322406 |
| a molecular-beacon-based asymmetric pcr assay for easy visualization of amplicons in the diagnosis of trichomoniasis. | the currently available nucleic acid amplification tests (naats) for trichomoniasis are accurate, quick and confirmative with superior sensitivity than traditional culture-based microbiology assays. however, these assays are associated with problems of carry over contamination, false positive results, requirement of technical expertise for performance and detection of end product. hence, a diagnostic assay with easy visualization of the amplified product will be profitable. an in-house, rapid, s ... | 2016 | 27318568 |
| molecular characterization of double-stranded rna virus in trichomonas vaginalis egyptian isolates and its association with pathogenicity. | trichomoniasis is a common human sexually transmitted infection caused by trichomonas vaginalis. the parasite can be infected with double-stranded rna viruses (tvv). this viral infection may have important implications on trichomonal virulence and disease pathogenesis. this study aimed to determine the prevalence of t. vaginalis virus among isolates obtained from infected (symptomatic and asymptomatic) women in ismailia city, egypt, and to correlate the virus-infected isolates with the clinical ... | 2016 | 27316695 |
| maldi-tof mass spectrometry as a potential tool for trichomonas vaginalis identification. | trichomonas vaginalis is a flagellated protozoan causing trichomoniasis, a sexually transmitted human infection, with around 276.4 million new cases estimated by world health organization. culture is the gold standard method for the diagnosis of t. vaginalis infection. recently, immunochromatographic assays as well as pcr assays for the detection of t. vaginalis antigen or dna, respectively, have been also available. although the well-known genome sequence of t. vaginalis has made possible the a ... | 2016 | 27282151 |
| novel hydrogenosomes in the microaerophilic jakobid stygiella incarcerata. | mitochondrion-related organelles (mros) have arisen independently in a wide range of anaerobic protist lineages. only a few of these organelles and their functions have been investigated in detail, and most of what is known about mros comes from studies of parasitic organisms such as the parabasalid trichomonas vaginalis here, we describe the mro of a free-living anaerobic jakobid excavate, stygiella incarcerata we report an rnaseq-based reconstruction of s. incarcerata's mro proteome, with an a ... | 2016 | 27280585 |
| in response to 'successful treatment of refractory trichomonas vaginalis infection using intravenous metronidazole' by hawkins et al. | | 2016 | 27278450 |
| evaluation of presto(plus) assay and lightmix kit trichomonas vaginalis assay for detection of trichomonas vaginalis in dry vaginal swabs. | this is an evaluation study of the presto(plus) assay for t. vaginalis by comparing to the tib molbiol lightmix kit trichomonas vaginalis assay using 615 dry collected vaginal and rectal swabs. discordant samples were analyzed by the qiagen® microbial dna qpcr for tv assay. both assays showed comparable performances (mcnemar p>0.05). | 2016 | 27268968 |
| rapid diagnosis of trichomonas vaginalis by testing vaginal swabs in an isothermal helicase-dependent amplivue assay. | the amplivue trichomonas assay (quidel) is a new federal drug administration-cleared rapid test for qualitative detection of trichomonas vaginalis (tv) dna in female vaginal specimens. the assay is based on biohelix's helicase-dependent amplification isothermal technology in conjunction with a disposable lateral-flow detection device, with a total turnaround time of approximately 45 minutes. | 2016 | 27196258 |
| optimization of trichomonas vaginalis diagnosis during pregnancy at a university hospital, argentina. | the aim of this study was to evaluate different methods for trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. a total of 386 vaginal exudates from pregnant women were analyzed. t. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged may-grunwald giemsa (mgg) staining, and wet mount with sodium-acetate-formalin (saf)/methylene blue method. pcr for 18s rrna gene as wel ... | 2016 | 27180578 |
| prevalence of trichomoniasis by pcr in women attending health screening in korea. | trichomoniasis is the most common curable sexually-transmitted infection (sti) worldwide. there are few reports on the prevalence of trichomonas vaginalis in korea. the purpose of this study was to examine the prevalence of trichomoniasis by pcr in guri city, korea. all adult women who visited hanyang university guri hospital for health screening within the national health care service were invited to participate in the study, and 424 women were enrolled between march and june 2011. pcr was used ... | 2016 | 27180577 |
| inflammatory responses in a benign prostatic hyperplasia epithelial cell line (bph-1) infected with trichomonas vaginalis. | trichomonas vaginalis causes the most prevalent sexually transmitted infection worldwide. trichomonads have been detected in prostatic tissues from prostatitis, benign prostatic hyperplasia (bph), and prostate cancer. chronic prostatic inflammation is known as a risk factor for prostate enlargement, benign prostatic hyperplasia symptoms, and acute urinary retention. our aim was to investigate whether t. vaginalis could induce inflammatory responses in cells of a benign prostatic hyperplasia epit ... | 2016 | 27180569 |
| detecting asymptomatic trichomonas vaginalis in females using the bd probetec™ trichomonas vaginalis q(x) nucleic acid amplification test. | trichomonas vaginalis (tv) rates in women are increasing and many are asymptomatic. nucleic acid amplification tests (naats) are becoming the 'gold standard' for diagnosis. we aimed to establish our asymptomatic tv rates by testing all women attending oxfordshire's sexual health service, regardless of symptoms, using the bd probetec™ tv q(x) naats (bdq(x)). during bdq(x)'s verification process, the sensitivity and specificity were calculated using results of 220 endocervical samples from symptom ... | 2017 | 27150360 |
| trichomonas vaginalis ntpdase and ecto-5'-nucleotidase hydrolyze guanine nucleotides and increase extracellular guanosine levels under serum restriction. | trichomonas vaginalis is the aethiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease in the world. the purinergic signaling pathway is mediated by extracellular nucleotides and nucleosides that are involved in many biological effects as neurotransmission, immunomodulation and inflammation. extracellular nucleotides can be hydrolyzed by a family of enzymes known as ectonucleotidases including the ecto-nucleoside triphosphate diphosphohydrolases (e-ntpdases) fam ... | 2016 | 27150347 |
| trichomonas vaginalis repair of iron centres proteins: the different role of two paralogs. | trichomonas vaginalis, the causative parasite of one of the most prevalent sexually transmitted diseases is, so far, the only protozoan encoding two putative repair of iron centres (ric) proteins. homologs of these proteins have been shown to protect bacteria from the chemical stress imposed by mammalian immunity. in this work, the biochemical and functional characterisation of the t. vaginalis rics revealed that the two proteins have different properties. expression of ric1 is induced by nitros ... | 2016 | 27124376 |
| structural investigation and inhibitory response of halide on phosphoserine aminotransferase from trichomonas vaginalis. | phosphoserine aminotransferase (psat) catalyses the second reversible step of the phosphoserine biosynthetic pathway in trichomonas vaginalis, which is crucial for the synthesis of serine and cysteine. | 2016 | 27102280 |
| in vitro susceptibility of iranian isolates of trichomonas vaginalis to metronidazole. | metronidazole, a 5-nitroimidazole derivative, is the main antitrichomonal agent of choice for treatment of trichomoniasis. since 1962, some cases of treatment failure with metronidazole have been reported and recently drug resistance is now on the rise in the world. this study was aimed to determine current susceptibility of iranian isolates of trichomonas vaginalis to metronidazole. | 2017 | 27095968 |
| a prospective study of trichomonas vaginalis and prostate cancer risk among african american men. | african americans (aa) have a higher prevalence of trichomonas vaginalis (tv) infection and a higher prostate (pc) risk. past studies suggest an association between tv seropositivity and pc, and therefore we prospectively investigated this association among aa men. | 2016 | 27091219 |
| role of disulfide linkage in action of bis(dialkylaminethiocarbonyl)disulfides as potent double-edged microbicidal spermicide: design, synthesis and biology. | trichomoniasis and candidiasis are amongst the most common morbidity-causing reproductive tract infections, generally treated by metronidazole and fluconazole respectively. poor vaginal efficacy, drug-resistance and non-spermicidal nature limit their use as topical microbicidal contraceptives. bis(dialkylaminethiocarbonyl)disulfides (4-38) were designed as dually active, non-surfactant molecules capable of eliminating trichomonas vaginalis and candida strains as well as irreversibly immobilizing ... | 2016 | 27084496 |
| evaluation of the in vitro activity of ceragenins against trichomonas vaginalis. | trichomonosis, caused by the protozoan parasite trichomonas vaginalis, is a curable sexually transmitted disease that is most commonly encountered worldwide. increasing importance of trichomoniasis and emerging of resistance against metronidazole lead to search for alternative drugs with different mode of activity. the purpose of this study was to determine in vitro activity of ceragenins (csa-13, csa-44, csa-13, and csa-138) against the metronidazole-susceptible (atcc 30001) and metronidazole-r ... | 2016 | 27078662 |
| are the fatty acids responsible for the higher effect of oil and alcoholic extract of nigella sativa over its aqueous extract on trichomonas vaginalis trophozoites? | trichomoniasis, the disease caused by the flagellate protozoan trichomonas vaginalis is the sexually transmitted infection with the largest annual incidence. metronidazole is the drug of choice recommended for the treatment of human trichomoniasis but it can lead to drug resistance and many other adverse effects. so, it is necessary for new alternatives for the treatment of this infection. medicinal plants or herbs could be good alternative regimens to be inexpensive, effective and safe to use. ... | 2016 | 27065592 |
| caatinga plants: natural and semi-synthetic compounds potentially active against trichomonas vaginalis. | trichomonas vaginalis causes trichomoniasis; the most common but overlooked non-viral sexually transmitted disease worldwide. the treatment is based at 5'-nitroimidazoles, however, failure are related to resistance of t. vaginalis to chemotherapy. caatinga is a uniquely brazilian region representing a biome with type desert vegetation and plants present diverse biological activity, however, with few studies. the aim of this study was to investigate the activity against t. vaginalis of different ... | 2016 | 27020521 |
| antichagasic and trichomonacidal activity of 1-substituted 2-benzyl-5-nitroindazolin-3-ones and 3-alkoxy-2-benzyl-5-nitro-2h-indazoles. | two series of new 5-nitroindazole derivatives, 1-substituted 2-benzylindazolin-3-ones (6-29, series a) and 3-alkoxy-2-benzyl-2h-indazoles (30-37, series b), containing differently functionalized chains at position 1 and 3, respectively, have been synthesized starting from 2-benzyl-5-nitroindazolin-3-one 5, and evaluated against the protozoan parasites trypanosoma cruzi and trichomonas vaginalis, etiological agents of chagas disease and trichomonosis, respectively. many indazolinones of series a ... | 2016 | 27017556 |
| trichomonas vaginalis induces il-1β production in a human prostate epithelial cell line by activating the nlrp3 inflammasome via reactive oxygen species and potassium ion efflux. | trichomonas vaginalis is a sexually transmitted protozoan parasite that causes vaginitis in women, and urethritis and prostatitis in men. il-1β is synthesized as immature pro-il-1β, which is cleaved by activated caspase-1. caspase-1 is, in turn, activated by a multi-protein complex known as an inflammasome. in this study, we investigated the inflammatory response of a prostate epithelial cell line (rwpe-1) to t. vaginalis and, specifically, the capacity of t. vaginalis to activate the nlrp3 infl ... | 2016 | 26959386 |
| influence of 120 kda pyruvate:ferredoxin oxidoreductase on pathogenicity of trichomonas vaginalis. | trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. however, the exact mechanism was not clarified. in this study, the author investigated whether the 120 kda protein of t. vaginalis may be involved in pathogenicity of trichomonads. antibodies against 120 kda protein of t. vaginalis, which was identifie ... | 2016 | 26951982 |
| correlation between trichomonas vaginalis and concurrency: an ecological study. | objective. there is a large variation in the prevalence of trichomonas vaginalis (tv) between different countries and between racial groups within countries. sexual partner concurrency may play a role. we investigate the correlation between the prevalence of sexual partner concurrency and tv prevalence. methods. spearman's correlation to assess relationship between tv prevalence in women and point prevalence of concurrency in men in (1) 11 countries with comparable data (concurrency data from wh ... | 2016 | 26949392 |
| mechanisms of tritrichomonas foetus pathogenicity in cats with insights from venereal trichomonosis. | almost 20 years has passed since trichomonosis was first recognized as a potential cause of diarrhea in domestic cats. despite progress in confirming disease causation, developing means for diagnosis, and identifying approaches to treatment of the infection, we still know very little about how this parasite causes diarrhea. with increasing recognition of resistance of trichomonosis to treatment with 5-nitroimidazole drugs, new treatment strategies based on an understanding of disease pathogenesi ... | 2017 | 26946069 |
| recent advances in the trichomonas vaginalis field. | the microaerophilic protist parasite trichomonas vaginalis is occurring globally and causes infections in the urogenital tract in humans, a condition termed trichomoniasis. in fact, trichomoniasis is the most prevalent non-viral sexually transmitted disease with more than 250 million people infected every year. although trichomoniasis is not life threatening in itself, it can be debilitating and increases the risk of adverse pregnancy outcomes, hiv infection, and, possibly, neoplasias in the pro ... | 2016 | 26918168 |
| comparison of permanent staining methods for the laboratory diagnosis of trichomoniasis. | trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (std) in the world. the diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. the aim of this study was to compare the performance of different fixing and staining techniques used in the detection of t. vaginalis in urine. the smears were fixed and submitted to different methods of permanent staining and then, the morphologica ... | 2016 | 26910452 |
| hiv risk and behaviour among part-time versus professional fsw: baseline report of an interventional cohort in burkina faso. | to readjust hiv control programmes in africa, we assessed the factors associated with high-risk behaviours and hiv infection among young female sex workers (fsw) in burkina faso. | 2016 | 26834073 |
| inflammatory response of a prostate stromal cell line induced by trichomonas vaginalis. | while trichomonas vaginalis, a cause of sexually transmitted infection, is known as a surface-dwelling protozoa, trichomonads have been detected in prostatic tissue from benign prostatic hyperplasia and prostatitis by immunoperoxidase assay or pcr. however, the immune response of prostate stromal cells infected with t. vaginalis has not been investigated. our objective was to investigate whether t. vaginalis could induce an inflammatory response in prostate stromal cells. incubation of a human p ... | 2016 | 26832322 |
| a new strategy for trichomonas testing female adolescents in the emergency department. | sensitive trichomonas diagnostic testing has become available, including nucleic acid amplification tests (naats) and a rapid antigen test. the study purpose was to determine if adding sensitive trichomonas testing to routine female sexually transmitted infection (sti) evaluations would increase trichomonas identification and treatment. | 2016 | 26820440 |
| trichomonas vaginalis infection and risk of advanced prostate cancer. | the epidemiologic evidence for an association of trichomonas vaginalis (tv) with overall prostate cancer is mixed, but some studies suggest tv may increase risk of more aggressive disease. the aim of this study was to assess whether tv serostatus is associated with advanced or fatal prostate cancer. | 2016 | 26818005 |
| osmc and incomplete glycine decarboxylase complex mediate reductive detoxification of peroxides in hydrogenosomes of trichomonas vaginalis. | osmotically inducible protein (osmc) and organic hydroperoxide resistance protein (ohr) are small, thiol-dependent peroxidases that comprise a family of prokaryotic protective proteins central to the defense against deleterious effects of organic hydroperoxides, which are reactive molecules that are formed during interactions between the host immune system and pathogens. trichomonas vaginalis, a sexually transmitted parasite of humans, possesses osmc homologues in its hydrogenosomes, anaerobic m ... | 2016 | 26794804 |
| trichomonas vaginalis: an updated overview towards diagnostic improvement. | the protozoan trichomonas vaginalis (tv) is responsible for trichomonosis, a sexually transmitted disease (std) with a significant incidence worldwide. this infection is one of the most common non-viral stds, representing almost 50% of all curable stds. trichomonosis has an incidence of 180 million new cases worldwide. nowadays, the 'gold standard' for tv diagnosis remains the use of in vitro cultures combined with daily visual microscopic evaluations, which is a time-consuming and low sensitive ... | 2016 | 26751868 |
| amaurocine: anti-trichomonas vaginalis protein produced by the basidiomycete amauroderma camerarium. | trichomonas vaginalis is the causative agent of trichomoniasis, the most common nonviral std worldwide. this infection can lead to severe health conditions, especially when women are affected. metronidazole and tinidazole are the only choices of treatment. in this sense, natural bioactive compounds against t. vaginalis are an interesting approach in the search for more efficient therapies. herein, amaurocine, a 12 kda protein, produced by the mushroom amauroderma camerarium was purified and test ... | 2016 | 26706604 |
| rna-binding proteins in trichomonas vaginalis: atypical multifunctional proteins. | iron homeostasis is highly regulated in vertebrates through a regulatory system mediated by rna-protein interactions between the iron regulatory proteins (irps) that interact with an iron responsive element (ire) located in certain mrnas, dubbed the ire-irp regulatory system. trichomonas vaginalis, the causal agent of trichomoniasis, presents high iron dependency to regulate its growth, metabolism, and virulence properties. although t. vaginalis lacks irps or proteins with aconitase activity, po ... | 2015 | 26703754 |
| a preliminary evaluation of a new genexpert (gx) molecular point-of-care test for the detection of trichomonas vaginalis. | global concerns regarding the prevalence, asymptomatic nature and burden of disease associated with trichomonas vaginalis (tv) continue. the lack of a portable molecular point-of-care assay to detect this infectious disease has meant that many remote or low-resource settings still need to rely on delayed results from central laboratories and/or syndromic management as treatment strategies. we evaluated the new genexpert (gx) tv nucleic acid amplification test (naat) compared with an in-house lab ... | 2016 | 26702132 |
| design, synthesis and biological evaluation of chalconyl blended triazole allied organosilatranes as giardicidal and trichomonacidal agents. | a series of chalconyl blended triazole allied silatranes (7a-g/8a-g/9a-g) were synthesized in good yields using a simple, economical and biocompatible synthetic route. the blend of three different pharmacologically active moieties into a single scaffold resulted into synergistic effect in their bio-activity. various substitutions were tried to study the structure activity relationship (sar) of the synthesized compounds on the basis of biological results. all the newly synthesized compounds were ... | 2016 | 26695730 |
| a trichomonas vaginalis rhomboid protease and its substrate modulate parasite attachment and cytolysis of host cells. | trichomonas vaginalis is an extracellular eukaryotic parasite that causes the most common, non-viral sexually transmitted infection worldwide. although disease burden is high, molecular mechanisms underlying t. vaginalis pathogenesis are poorly understood. here, we identify a family of putative t. vaginalis rhomboid proteases and demonstrate catalytic activity for two, tvrom1 and tvrom3, using a heterologous cell cleavage assay. the two t. vaginalis intramembrane serine proteases display differe ... | 2015 | 26684303 |
| novel structural features of the immunocompetent ceramide phospho-inositol glycan core from trichomonas vaginalis. | the ceramide phosphoinositol glycan core (cpi-gc) of the lipophosphoglycan of trichomonas vaginalis is a major virulent factor of this common genitourinary parasite. while its carbohydrate composition has been reported before, its structure has remained largely unknown. we isolated the glycan portions of cpi-gc by nitrous acid deamination and hydrofluoric acid treatment and investigated their structures by methylation analysis and 1- and 2-d nmr. we found that the α-anomer of galactose is a majo ... | 2016 | 26671321 |
| the past, present and future of fluorescent protein tags in anaerobic protozoan parasites. | the world health organization currently recognizes diarrhoeal diseases as a significant cause of death in children globally. protozoan parasites such as giardia and entamoeba that thrive in the oxygen-deprived environment of the human gut are common etiological agents of diarrhoea. in the urogenital tract of humans, the anaerobic protozoan parasite trichomonas vaginalis is notorious as the most common non-viral, sexually transmitted pathogen. even with high medical impact, our understanding of a ... | 2016 | 26653973 |
| [vulvovaginal trichomoniasis: epidemiology, clinical and parasitological characteristics]. | trichomonas vaginalis infection is the most prevalent nonviral sexual transmitted infection. the world health organization estimates that its prevalence is 170 million cases worldwide each year. in women, he represents the third cause of vaginitis. | 2015 | 26644101 |
| advances in the prevention of infection-related preterm birth. | infection-related preterm birth (ptb) is more common at early gestational ages and is associated with major neonatal mortality and morbidity. abnormal genital tract microflora in early pregnancy predicts late miscarriage and early ptb. accordingly, it is logical to consider antibiotics as an intervention. unfortunately, the conclusions of systematic reviews and meta-analyses (sr&mas) carried out in an attempt to explain the confusion over the heterogeneity of individual studies are flawed by the ... | 2015 | 26635788 |
| a diagnostic and symptomatological study on trichomoniasis in symptomatic pregnant women in rafsanjan, south central iran in 2012-13. | trichomonas vaginalis, the causative agent of trichomoniasis, is responsible for more than half of all sexually transmitted infections (stis). the present study aimed to determine the frequency of t. vaginalis infection and its clinical manifestations in symptomatic pregnant women in the area based on four different diagnostic methods. | 2017 | 26622305 |
| prevalence, clinical criteria and sociodemographic predictors of trichomonas vaginalis infection in suspected egyptian women, using direct diagnostic techniques. | the present study aimed to determine the prevalence and associated risk factors of vaginal trichomoniasis in women referred to gynecologic clinic in benha university hospital, egypt. | 2017 | 26622298 |
| substrate-induced dimerization of engineered monomeric variants of triosephosphate isomerase from trichomonas vaginalis. | the dimeric nature of triosephosphate isomerases (tims) is maintained by an extensive surface area interface of more than 1600 å2. tims from trichomonas vaginalis (tvtim) are held in their dimeric state by two mechanisms: a ball and socket interaction of residue 45 of one subunit that fits into the hydrophobic pocket of the complementary subunit and by swapping of loop 3 between subunits. tvtims differ from other tims in their unfolding energetics. in tvtims the energy necessary to unfold a mono ... | 2015 | 26618356 |
| adenosine/guanosine preferring nucleoside ribohydrolase is a distinct, druggable antitrichomonal target. | nucleoside salvage pathway enzymes used by trichomonas vaginalis are distinct from the pathway involved in activation of existing 5-nitroimidazole drugs. they thus represent excellent targets for developing novel, mechanism-based antitrichomonal agents. the purine-specific adenosine/guanosine preferring ribohydrolase (agnh) was screened against the nih clinical collection to assess its druggability. eight compounds, including five flavonoids, were identified with ic50 values ⩽10 μm and confirmed ... | 2015 | 26592812 |
| five putative nucleoside triphosphate diphosphohydrolase genes are expressed in trichomonas vaginalis. | trichomonas vaginalis is a protozoan that parasitizes the human urogenital tract causing trichomoniasis, the most common non-viral sexually transmitted disease. the parasite has unique genomic characteristics such as a large genome size and expanded gene families. ectonucleoside triphosphate diphosphohydrolase (e-ntpdase) is an enzyme responsible for hydrolyzing nucleoside tri- and diphosphates and has already been biochemically characterized in t. vaginalis. considering the important role of th ... | 2016 | 26590960 |
| trichomonas vaginalis lipophosphoglycan exploits binding to galectin-1 and -3 to modulate epithelial immunity. | trichomoniasis is the most common non-viral sexually transmitted infection caused by the vaginotropic extracellular protozoan parasite trichomonas vaginalis. the infection is recurrent, with no lasting immunity, often asymptomatic, and linked to pregnancy complications and risk of viral infection. the molecular mechanisms of immune evasion by the parasite are poorly understood. we demonstrate that galectin-1 and -3 are expressed by the human cervical and vaginal epithelial cells and act as patho ... | 2016 | 26589797 |
| correlates of trichomonas vaginalis among middle age and older adults who use drugs. | recent studies have reported high rates of trichomonas vaginalis among middle age and older adults. though trichomoniasis risk factors in this age cohort remain largely unknown, illicit drug use has been associated with increased incidence of sexually transmitted infections (stis). the number of mid-older adults using illicit drugs has increased significantly in recent years suggesting the need to understand the relationship between drug use and stis in this age cohort. | 2015 | 26583203 |
| genital trichomonas vaginalis is rare among female attendees at a sydney metropolitan sexual health clinic. | | 2016 | 26558330 |
| exploring the mechanism of how tvmyb2 recognizes and binds ap65-1 by molecular dynamics simulations and free energy calculations. | tvmyb2, one of the myb-like transcriptional factors in trichomonas vaginalis, binds to two closely spaced promoter sites, mre-1/mre-2r and mre-2f, on the ap65-1 gene. however, detailed dynamical structural characteristics of the tvmyb2-ap65-1 complex and a detailed study of the protein in the complex have not been done. focused on a specific tvmyb2-mre-2-13 complex (pdb code: ) and a series of mutants k51a, r84a and r87a, we applied molecular dynamics (md) simulation and molecular mechanics gene ... | 2016 | 26548411 |
| trichomonas vaginalis: pathogenicity and potential role in human reproductive failure. | trichomonas vaginalis, which colonizes the genitourinary tract of men and women, is a sexually transmitted parasite causing symptomatic or asymptomatic trichomoniasis. the host-parasite relationship is very complex, and clinical symptoms cannot likely be attributed to a single pathogenic effect. among the many factors responsible for interactions between t. vaginalis and host tissues, contact-dependent and contact-independent mechanisms are important in pathogenicity, as is the immune response. | 2016 | 26546373 |
| molecular typing of the actin gene of trichomonas vaginalis isolates by pcr-rflp in iran. | trichomonas vaginalis is a human urogenital pathogen that causes trichomoniasis, the most common nonviral, parasitic sexually transmitted infection in the world. at present, little is known regarding the degree of strain variability of t. vaginalis. a classification method for t. vaginalis strains would be a useful tool in the study of the epidemiology, drug resistance, pathogenesis and transmission of t. vaginalis. eight different types of actin genes have been identified by pcr-rflp in t. vagi ... | 2015 | 26542260 |
| activation of mapk is required for ros generation and exocytosis in hmc-1 cells induced by trichomonas vaginalis-derived secretory products. | trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with t. vaginalis. mitogen-activated protein kinase (mapk), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. in this study, we investigated whether mapk is invo ... | 2015 | 26537039 |