| in vitro antimicrobial effects of two antihalitosis mouth rinses on oral pathogens and human tongue microbiota. | the aim of the study was to compare the antimicrobial activity of a mouth rinse containing chlorhexidine and cetylpyridinium chloride (mr1) with a stannous fluoride-based mouth rinse (mr2) in vitro. | 2013 | 23368885 |
| detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women. | the aim of this study was to use the fluorescence in situ hybridization (fish) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. this cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque samples were collected and the fish ... | 2012 | 23018230 |
| bacterial antagonism against periodontopathogens. | the aim of the current study is to compare the prevalence of commensal bacteria, with beneficial properties, for healthy and diseased individuals and additionally to examine the inhibitory effect of some commercial dietary probiotics on periodontopathogens, comparing this inhibitory effect to that of orally derived beneficial bacteria. | 2013 | 22897652 |
| antibiotic susceptibility profiles of oral pathogens. | periodontitis is a bacterial disease that can be treated with systemic antibiotics. the aim of this study was to establish the antibiotic susceptibility profiles of five periodontal pathogens to six commonly used antibiotics in periodontics. a total of 247 periodontal bacterial isolates were tested for susceptibility to the six antibiotics using the etest method. mic(50) and mic(90) values (minimum inhibitory concentrations for 50% and 90% of the organisms, respectively) were calculated. both eu ... | 2012 | 22890195 |
| periodontal pathogen load and increased antibody response to heat shock protein 60 in patients with cardiovascular disease. | to determine the relationship between periodontal pathogen load and anti-human heat shock protein 60 (hhsp60) antibodies in patients with established cardiovascular disease (cvd). | 2012 | 22882677 |
| antimicrobial activity of nanoparticulate metal oxides against peri-implantitis pathogens. | dental plaque accumulation may result in peri-implantitis, an inflammatory process causing loss of supporting bone that may lead to dental implant failure. the antimicrobial activities of six metal and metal oxide nanoparticles and two of their composites against bacterial pathogens associated with peri-implantitis were examined under anaerobic conditions. the activities of nanoparticles of silver (ag), cuprous oxide (cu(2)o), cupric oxide (cuo), zinc oxide (zno), titanium dioxide (tio(2)), tung ... | 2012 | 22727529 |
| subgingival microbiota from cebus apella (capuchin monkey) with different periodontal conditions. | this present study evaluated the subgingival microbiota of the cebus apella with different periodontal conditions kept by the tufted capuchin monkey procreation center (são paulo state university - unesp) or free-ranging monkeys. for this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. the primates were submitted to periodontal evaluation and biofilm samples were transferred to vmga iii transpor ... | 2012 | 22710412 |
| effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. | emingil g, han b, özdemir g, tervahartiala t, vural c, atilla g, baylas h, sorsa t. the effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. j periodont res 2012; 47: 729-739. © 2012 john wiley & sons a/s background and objective: to study the effectiveness of azithromycin in combination with nonsurgical periodontal therapy on clinical and microbiological parame ... | 2012 | 22571226 |
| quantification of subgingival bacterial pathogens at different stages of periodontal diseases. | anaerobic gram-negative oral bacteria such as treponema denticola, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsythia, campylobacter rectus, and fusobacterium nucleatum are closely associated with periodontal diseases. we measured the relative population (bacterial levels) of these oral pathogens in subgingival tissues of patients at different stages of korean chronic periodontal diseases. we divided the individuals into those with chronic gingivitis (g), moder ... | 2012 | 22526568 |
| specificity of antimicrobial peptide ll-37 to neutralize periodontopathogenic lipopolysaccharide activity in human oral fibroblasts. | the antimicrobial peptide ll-37 is known to have a potent lipopolysaccharide (lps)-neutralizing activity in various cell types. because of observed heterogeneity within periodontopathogenic lps, the authors hypothesized that ll-37 had specificity to neutralize such lps activity. the present study, therefore, aims to investigate the lps-neutralizing activity of ll-37 to various periodontopathogenic lps in interleukin-8 (il-8) production after challenging them in human oral fibroblasts. | 2013 | 22443521 |
| comparative evaluation of the effects of different photoablative laser irradiation protocols on the gingiva of periodontopathic patients. | we aimed at quantifying the presence of periodontopathogens in gingival biopsies from periodontitis patients treated with different photoablative lasers (diode gaas, er:yag, nd:yag, and co(2) lasers) and histologically analyzing their effects on the gingiva. | 2012 | 22401128 |
| [effect of periodontal surgery on the subgingival periodontal microbiota in artificial class iii furcation defects: an experimental study in monkeys]. | this study was to longitudinally evaluate the change of prevalence of five periodontal putative pathogens in the subgingival plaque of artificial class iii furcation defects at the three time-points, including before the establishment of furcation defects, before and 6 months after periodontal surgery. | 2012 | 22353894 |
| azithromycin as an adjunctive treatment of generalized severe chronic periodontitis: clinical, microbiologic, and biochemical parameters. | this study examines the efficacy of azithromycin in combination with non-surgical periodontal therapy on clinical and microbiologic parameters and gingival crevicular fluid (gcf) matrix metalloproteinases-8 (mmp-8) levels over 6 months in patients with severe generalized chronic periodontitis (cp). | 2012 | 22324488 |
| release of metronidazole from electrospun poly(l-lactide-co-d/l-lactide) fibers for local periodontitis treatment. | we aimed to achieve detailed biomaterials characterization of a drug delivery system for local periodontitis treatment based on electrospun metronidazole-loaded resorbable polylactide (pla) fibers. | 2012 | 22226009 |
| investigation and quantification of key periodontal pathogens in patients with type 2 diabetes. | diabetes is a recognized risk factor for periodontitis. there are conflicting data regarding whether healthy diabetic patients or diabetic patients with chronic periodontitis have an altered subgingival microbiota compared with nondiabetic individuals. the aim of the present study was to detect quantitative differences in selected periodontopathogens in the subgingival plaque of diabetic patients using taqman quantitative pcr. | 2012 | 22220967 |
| adhesion of aggregatibacter actinomycetemcomitans and fusobacterium nucleatum on bioactive tio(2) surfaces. | bioactive tio(2) coatings have been found to enhance fibroblast adhesion and gingival attachment on the titanium surfaces, but no information is available whether the coatings also promote the adhesion of periodontal pathogens. | 2013 | 22220677 |
| the antibacterial activity of various saturated and unsaturated fatty acids against several oral pathogens. | the antibacterial activity of various saturated fatty acids (sfa) and unsaturated fatty acids (usfa) against different oral pathogens which are implicated in the cause of dental caries, stomatitis, gingivitis, and periodontitis was examined. the saturated fatty acids pa, sta and ara, and the unsaturated omega-7 fatty acids pla and omega-9 fatty acids oa showed either none to low antimicrobial activity against all of the 12 oral pathogenic strains used in this study. in contrast, the omega-3 pufa ... | 2013 | 24640241 |
| reactivation of latent hiv-1 by a wide variety of butyric acid-producing bacteria. | latently infected cells harbor human immunodeficiency virus type 1 (hiv-1) proviral dna copies integrated in heterochromatin, allowing persistence of transcriptionally silent proviruses. it is widely accepted that hypoacetylation of histone proteins by histone deacetylases (hdacs) is involved in maintaining the hiv-1 latency by repressing viral transcription. hiv-1 replication can be induced from latently infected cells by environmental factors, such as inflammation and co-infection with other m ... | 2012 | 22322557 |
| distinct polymicrobial populations in a chronic foot ulcer with implications for diagnostics and anti-infective therapy. | polymicrobial infections caused by combinations of different bacteria are being detected with an increasing frequency. the evidence of such complex infections is being revealed through the use of novel molecular and culture-independent methods. considerable progress has been made in the last decade regarding the diagnostic application of such molecular techniques. in particular, 16s rdna-based sequencing and even metagenomic analyses have been successfully used to study the microbial diversity i ... | 2014 | 24679105 |
| randomized in vivo evaluation of photodynamic antimicrobial chemotherapy on deciduous carious dentin. | the aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (rmgic)], ledtb [photodynamic antimicrobial chemotherapy (pact) with light-emitting diode associated with toluidine blue solution and rmgic], and lmb ... | 2015 | 26502235 |
| development of quantitative real-time pcr primers for detecting 42 oral bacterial species. | in this study, we introduced species-specific quantitative real-time pcr (qpcr) primers designed based on a dna-dependent rna polymerase beta-subunit gene (rpob) for detecting 42 oral bacterial species. the specificity of the qpcr primers was confirmed by conventional pcr with the genomic dnas of 73-79 strains regarding 73-75 bacterial species including the type strain for the target species. the standard curves revealed the lower detection limits of 42 bacterial species-specific qpcr primers ra ... | 2013 | 23689247 |
| influence of biofilm formation by gardnerella vaginalis and other anaerobes on bacterial vaginosis. | bacterial vaginosis (bv) is the worldwide leading vaginal disorder among women of reproductive age. bv is characterized by the replacement of beneficial lactobacilli and the augmentation of anaerobic bacteria. gardnerella vaginalis is a predominant bacterial species, but bv is also associated with other numerous anaerobes, such as atopobium vaginae, mobiluncus mulieris, prevotella bivia, fusobacterium nucleatum, and peptoniphilus species. currently, the role of g. vaginalis in the etiology of bv ... | 2015 | 26080369 |
| [noma/cancrum oris: a neglected disease]. | noma is an aggressive orofacial gangrenous pathology that damages hard and soft tissues of the mouth and the face. throughout the centuries it has been present around the globe, but nowadays it has practically disappeared from developed countries and mainly affects children from the most disadvantaged places, especially in africa. noma disease is a multifactorial process; malnutrition, debilitating diseases (bacterial or viral systemic diseases, hiv-associated immunosuppression, etc.) and intrao ... | 2015 | 26437752 |
| identification of an antibacterial protein by functional screening of a human oral metagenomic library. | screening of a bacterial artificial chromosome (bac) library containing metagenomic dna from human plaque and saliva allowed the isolation of four clones producing antimicrobial activity. three of these were pigmented and encoded homologues of glutamyl-trna reductase (glutr), an enzyme involved in the c5 pathway leading to tetrapyrole synthesis, and one clone had antibacterial activity with no pigmentation. the latter contained a bac with an insert of 15.6 kb. initial attempts to localize the ge ... | 2015 | 26347298 |
| modulation of quaternary structure and enhancement of ligand binding by the k-turn of tandem glycine riboswitches. | most known glycine riboswitches have two homologous aptamer domains arranged in tandem and separated by a short linker. the two aptamers associate through reciprocal "quaternary" interactions that have been proposed to result in cooperative glycine binding. recently, the interaptamer linker was found to form helix p0 with a previously unrecognized segment 5' to the first aptamer domain. p0 was shown to increase glycine affinity, abolish cooperativity, and conform to the k-turn motif consensus. w ... | 2013 | 23249744 |
| fecal bacteria act as novel biomarkers for noninvasive diagnosis of colorectal cancer. | gut microbiota have been implicated in the development of colorectal cancer. we evaluated the utility of fecal bacterial marker candidates identified by our metagenome sequencing analysis for colorectal cancer diagnosis. | 2016 | 27697996 |
| collateral damage: insights into bacterial mechanisms that predispose host cells to cancer. | infections are estimated to contribute to 20% of all human tumours. viruses are known to induce cell transformation, but evidence has also linked bacteria, such as helicobacter pylori and salmonella enterica subsp. enterica serovar typhi, to different cancer types. in addition, chlamydia trachomatis, fusobacterium nucleatum and bacteroides fragilis are associated with the development of cancer, although a causal relationship has not yet been established. bacterial effectors such as colibactin an ... | 2017 | 28045107 |
| gut microbiota, inflammation, and colorectal cancer. | colorectal cancer is the second-leading cause of cancer-related deaths in the united states and fourth-leading cause of cancer-related deaths worldwide. while cancer is largely considered to be a disease of genetic and environmental factors, increasing evidence has demonstrated a role for the microbiota (the microorganisms associated with the human body) in shaping inflammatory environments and promoting tumor growth and spread. herein, we discuss both human data from meta'omics analyses and dat ... | 2016 | 27607555 |
| could gut microbiota serve as prognostic biomarker associated with colorectal cancer patients' survival? a pilot study on relevant mechanism. | evidences have shown that dysbiosis could promote the progression of colorectal cancer (crc). however, the association of dysbiosis and prognosis of crc is barely investigated. therefore, we used 16s rrna gene sequencing approach to determine differences in microbiota among tumor tissues of different prognosis and found that fusobacterium nucleatum and bacteroides fragilis were more abundant in worse prognosis groups, while faecalibacterium prausnitzii displayed higher abundance in survival grou ... | 2016 | 27323816 |
| the myeloid immune signature of enterotoxigenic bacteroides fragilis-induced murine colon tumorigenesis. | enterotoxigenic bacteroides fragilis (etbf), a human commensal and candidate pathogen in colorectal cancer (crc), is a potent initiator of interleukin-17 (il-17)-dependent colon tumorigenesis in min(apc+/-) mice. we examined the role of il-17 and etbf on the differentiation of myeloid cells into myeloid-derived suppressor cells (mdscs) and tumor-associated macrophages, which are known to promote tumorigenesis. the myeloid compartment associated with etbf-induced colon tumorigenesis in min mice w ... | 2017 | 27301879 |
| [how some commensal bacteria would exacerbate colorectal carcinogenesis?]. | the gut microbiota maintains a relationship with its host with strong mutual benefits. changes in the composition of the intestinal microbiota have been detected in colorectal cancer patients to the extent that it is now considered as a real contributing factor in this pathology. in this review, we focus on three commensal bacterial species, namely bacteroides fragilis, fusobacterium nucleatum, and escherichia coli, which seem to emerge as pathogens and to contribute to colorectal carcinogenesis ... | 2016 | 26936175 |
| high occurrence of fusobacterium nucleatum and clostridium difficile in the intestinal microbiota of colorectal carcinoma patients. | colorectal carcinoma is considered the fourth leading cause of cancer deaths worldwide. several microorganisms have been associated with carcinogenesis, including enterococcus spp., helicobacter pylori, enterotoxigenic bacteroides fragilis, pathogenic e. coli strains and oral fusobacterium. here we qualitatively and quantitatively evaluated the presence of oral and intestinal microorganisms in the fecal microbiota of colorectal cancer patients and healthy controls. seventeen patients (between 49 ... | 2016 | 26691472 |
| the gut microbial community in metabolic syndrome patients is modified by diet. | intestinal microbiota changes may be involved in the development of metabolic syndrome (mets), which is a multicomponent disorder frequently associated with obesity. the aim of this study was to test the effect of consuming two healthy diets: a mediterranean diet and a low-fat high-carbohydrate diet, for 2years in the gut microbiota of mets patients and those in the control group. we analyzed the differences in the bacterial community structure between the groups after 2years of dietary interven ... | 2016 | 26376027 |
| in vitro activity of biapenem plus rpx7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria. | biapenem is a carbapenem being developed in combination with rpx7009, a new inhibitor of serine β-lactamases. biapenem was tested alone and in combination with fixed concentrations of rpx7009 by agar dilution against 377 recent isolates of anaerobes. a separate panel of 27 isolates of bacteroides spp. with decreased susceptibility or resistance to imipenem was also tested. comparator drugs included meropenem, piperacillin-tazobactam, ampicillin-sulbactam, cefoxitin, ceftazidime, metronidazole, c ... | 2013 | 23529731 |
| bacterial pathogens related to chronic suppurative otitis media in individuals with cleft palate: bacteriological culture and polymerase chain reaction. | to characterize the microbial etiology of chronic suppurative otitis media comparing the methods of classical bacteriological culture and polymerase chain reaction. | 2014 | 22594401 |
| stress hormone epinephrine (adrenaline) and norepinephrine (noradrenaline) effects on the anaerobic bacteria. | microbial endocrinology is a relatively new research area that already encompasses the anaerobes. stress hormones, epinephrine and norepinephrine, can affect the growth of anaerobic bacteria such as fusobacterium nucleatum, prevotella spp., porhyromonas spp., tanerella forsythia and propionibacterium acnes and can increase virulence gene expression, iron acquisition and many virulence factors of some anaerobic species such as clostridium perfringens, porphyromonas gingivalis and brachyspira pilo ... | 2017 | 28077337 |
| immunologic characteristics of human gingival fibroblasts in response to oral bacteria. | there is ample evidence that gingival fibroblasts (gfs) participate in the immune response to oral bacteria and serve as immune-regulatory cells. the objective of this study was to investigate the innate immune response of gfs to oral bacteria. | 2016 | 27558278 |
| a microbiological profile of unexposed and exposed pulp space of primary endodontic infections by checkerboard dna-dna hybridization. | the aim of this study was to evaluate, by checkerboard dna-dna hybridization, the composition of the microbiota of primary endodontic infections in cases associated with exposed (n = 30) and unexposed (n = 30) pulp space. | 2012 | 22703649 |
| energetics of pathogenic bacteria and opportunities for drug development. | the emergence and spread of drug-resistant pathogens and our inability to develop new antimicrobials to overcome resistance has inspired scientists to consider new targets for drug development. cellular bioenergetics is an area showing promise for the development of new antimicrobials, particularly in the discovery of new anti-tuberculosis drugs where several new compounds have entered clinical trials. in this review, we have examined the bioenergetics of various bacterial pathogens, highlightin ... | 2014 | 25476763 |
| common polymorphisms in the ifi16 and aim2 genes are associated with periodontal disease. | we investigated the single nucleotide polymorphism (snp) context of a previously identified periodontitis-associated locus and examined its association with microbial, biological and periodontal disease clinical parameters. | 2017 | 28387608 |
| dental plaque microbial profiles of children from khartoum, sudan, with congenital heart defects. | few studies have focused on the bacterial species associated with the deterioration of the dental and gingival health of children with congenital heart defects (chd). the aims of this study were (1) to examine the dental plaque of children with chd in order to quantify bacterial load and altered bacterial composition compared with children without chd; and (2) to investigate the correlation between the level of caries and gingivitis and dental biofilm bacteria among those children. in this cross ... | 2017 | 28326155 |
| periodontal pathogens and risk of incident cancer in postmenopausal females: the buffalo osteoperio study. | extraoral translocation of oral bacteria may contribute to associations between periodontal disease and cancer. the associations among the presence of three orange-complex periodontal pathogens (fusobacterium nucleatum, prevotella intermedia, and campylobacter rectus), two red-complex periodontal pathogens (porphyromonas gingivalis and tannerella forsythia), and cancer risk were investigated. | 2016 | 26513268 |
| microbiota at teeth and implants in partially edentulous patients. a 10-year retrospective study. | to determine the microbiota at implants and adjacent teeth 10 years after placement of implants with a sandblasted and acid-etched surface. | 2016 | 25827437 |
| relationship between halitosis and periodontal disease - associated oral bacteria in tongue coatings. | the objective of our study was to investigate the relationship between halitosis and oral bacteria in tongue coating (tc) and saliva samples from patients with halitosis, and to evaluate the effect of tongue cleaning on halitosis. | 2014 | 23890391 |
| validation of antibiotic efficacy on in vitro subgingival biofilms. | systemic antibiotics are often used as adjunctive treatment modalities for periodontal diseases. testing of antimicrobial efficacy can be relevant only if the bacteria are in the form of biofilms rather than the planktonic state, and at concentrations of physiologic relevance, i.e., reachable in the periodontal pocket. the aim of the present study is to test the antimicrobial efficacy of five common antibiotic schemes at physiologically relevant concentrations on a multispecies in vitro biofilm ... | 2014 | 23659420 |
| preventive effects of houttuynia cordata extract for oral infectious diseases. | houttuynia cordata (hc) (saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in japan. our recent survey showed that hc poultice (hcp) prepared from smothering fresh leaves of hc had been frequently used for the treatment of purulent skin diseases with high effectiveness. our experimental study also demonstrated that ethanol extract of hcp (ehcp) has antibacterial, antibiofilm, and anti-inflammatory effects against s. aureus which ca ... | 2016 | 27413739 |
| effects of repeated screw tightening on implant abutment interfaces in terms of bacterial and yeast leakage in vitro: one-time abutment versus the multiscrewing technique. | screw loosening can damage the interfaces of implant components, resulting in susceptibility to contamination of the internal parts by microorganisms. the aim of this study was to investigate the impact of abutment screw retightening on the leakage of two different types of bacteria, streptococcus sanguinis and fusobacterium nucleatum, and of the yeast candida albicans. two types of implant-abutment systems with tube-in-tube interfaces were tested. groups a and b each used a different type of sy ... | 2016 | 26901305 |
| effects of surface reaction-type pre-reacted glass ionomer on oral biofilm formation of streptococcus gordonii. | streptococcus gordonii, a bacterium involved in the initial colonization of tooth surfaces, contributes to dental biofilm formation and is an important cause of infective endocarditis. this study aimed to investigate the influence of surface reaction-type pre-reacted glass ionomer (s-prg) filler on oral bacterial growth and aggregation of s. gordonii. the effect of various concentrations of s-prg eluate on the growth and the biofilm formation of s. gordonii and other oral microorganisms (strepto ... | 2016 | 26319990 |
| in vitro growth-inhibitory effect of ethanol gras plant and supercritical co₂ hop extracts on planktonic cultures of oral pathogenic microorganisms. | conventional chemical antiseptics used for treatment of oral infections often produce side-effects, which restrict their long-term use. plants are considered as perspective sources of novel natural antiseptics. however, little is still known about their inhibitory properties against oral pathogens. the objective of this study was to test in vitro antimicrobial activities of generally recognized as safe (gras) species against planktonic cultures of cariogenic, periodontal and candidal microorgani ... | 2015 | 26232134 |
| cellular components mediating coadherence of candida albicans and fusobacterium nucleatum. | candida albicans is an opportunistic fungal pathogen found as part of the normal oral flora. it can be coisolated with fusobacterium nucleatum, an opportunistic bacterial pathogen, from oral disease sites, such as those involved in refractory periodontitis and pulp necrosis. the physical coadherence between these 2 clinically important microbes has been well documented and suggested to play a role in facilitating their oral colonization and colocalization and contributing to polymicrobial pathog ... | 2015 | 26152186 |
| microflora in oral ecosystems and salivary secretion rates--a 3-year follow-up after radiation therapy to the head and neck region. | results indicate that late improvements of radiation therapy (rt) in the head and neck region may diminish the long-term effects on salivary glands and oral microflora. the aim was therefore to analyze salivary secretion rates and oral microflora over time in rt subjects. | 2015 | 26058004 |
| [features of adhesion of anaerobic periodontopathogenic bacteria and candida albicans fungi to experimental samples of basis dental plastic depending on surface roughness and polishing method]. | study the main surface parameters of milled polyacrylic materials using atomic force microscopy and primary microbial adhesion of periodontopathogenic group bacteria and candida albicans fungi taking into consideration the method of sample polishing. | 2016 | 25816509 |
| antimicrobial and immunomodulatory properties of pgla-am1, cpf-am1, and magainin-am1: potent activity against oral pathogens. | cationic amphipathic α-helical peptides are intensively studied classes of host defence peptides (hdps). three peptides, peptide glycine-leucine-amide (pgla-am1), caerulein-precursor fragment (cpf-am1) and magainin-am1, originally isolated from norepinephrine-stimulated skin secretions of the african volcano frog xenopus amieti (pipidae), were studied for their antimicrobial and immunomodulatory activities against oral and respiratory pathogens. minimal effective concentrations (mecs), determine ... | 2014 | 25447193 |
| action of essential oils from brazilian native and exotic medicinal species on oral biofilms. | essential oils (eo) obtained from twenty medicinal and aromatic plants were evaluated for their antimicrobial activity against the oral pathogens candida albicans, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus sanguis and streptococcus mitis. | 2014 | 25407737 |
| a comparative antimicrobial analysis of various root canal irrigating solutions on endodontic pathogens: an in vitro study. | evolution in understanding the poly-microbial environment of both endodontic infections and that of failed root canal treatments has been debatable over the years. the present study was designed to compare and analyze the effect of various root canal irrigation solutions on certain endodontic pathogens in vitro. | 2014 | 25095835 |
| microbial community in persistent apical periodontitis: a 16s rrna gene clone library analysis. | to characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. | 2015 | 25088120 |
| microbiological profile resistant to different intracanal medications in primary endodontic infections. | this clinical study aimed to determine the microbiological profile resistant to different intracanal medications in primary endodontic infections by using both microbiological culture and the checkerboard dna-dna hybridization technique. | 2015 | 25892513 |
| phototargeting human periodontal pathogens in vivo. | the effects of blue light at 455 nm were investigated on the bacterial composition of human dental plaque in vivo. eleven subjects who refrained from brushing for 3 days before and during phototherapy participated in the study. light with a power density of 70 mw/cm(2) was applied to the buccal surfaces of premolar and molar teeth on one side of the mouth twice daily for 2 min over a period of 4 days. dental plaque was harvested at baseline and again at the end of 4 days from eight posterior tee ... | 2015 | 24346334 |
| subgingival microbiome in smokers and non-smokers in korean chronic periodontitis patients. | smoking is a major environmental factor associated with periodontal diseases. however, we still have a very limited understanding of the relationship between smoking and subgingival microflora in the global population. here, we investigated the composition of subgingival bacterial communities from the pooled plaque samples of smokers and non-smokers, 134 samples in each group, in korean patients with moderate chronic periodontitis using 16s rrna gene-based pyrosequencing. a total of 17,927 reads ... | 2015 | 25283067 |
| advancement in the routine identification of anaerobic bacteria by maldi-tof mass spectrometry. | we evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) biotyper as a tool for the identification of anaerobic bacteria compared with 500 base-pair (bp) 16s ribosomal ribonucleic acid (rrna) gene sequencing analysis, which is considered to be the "gold standard" method. a total of 484 anaerobic bacteria were retrieved from the clinical specimens of 318 pediatric patients. molecular identification resulted in 18 genera and 51 species. the most prev ... | 2013 | 23584672 |
| comprehensive analysis of bacterial flora in postoperative maxillary cyst fluid by 16s rrna gene and culture methods. | intracystic fluid was aseptically collected from 11 patients with postoperative maxillary cyst (pomc), and dna was extracted from the pomc fluid. bacterial species were identified by sequencing after cloning of approximately 580 bp of the 16s rrna gene. identification of pathogenic bacteria was also performed by culture methods. the phylogenetic identity was determined by sequencing 517-596 bp in each of the 1139 16s rrna gene clones. a total of 1114 clones were classified while the remaining 25 ... | 2012 | 22685668 |
| bacteria in the apical root canals of teeth with apical periodontitis. | bacteria in the tooth root canal may cause apical periodontitis. this study examined the bacterial species present in the apical root canal of teeth with apical periodontitis. antibiotic sensitivity tests were performed to evaluate whether these identified bacterial species were susceptible to specific kinds of antibiotics. | 2016 | 27745799 |
| bacterial diversity of symptomatic primary endodontic infection by clonal analysis. | the aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. samples were collected from 10 patients and submitted to bacterial dna isolation, 16s rrna gene amplification, cloning, and sequencing. a bacterial genomic library was constructed and bacterial diversity was estimated. the mean number of taxa per canal was 15, ranging from 11 to 21. a total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) w ... | 2016 | 27737357 |
| advanced caries microbiota in teeth with irreversible pulpitis. | bacterial taxa in the forefront of caries biofilms are candidate pathogens for irreversible pulpitis and are possibly the first ones to invade the pulp and initiate endodontic infection. this study examined the microbiota of the most advanced layers of dentinal caries in teeth with irreversible pulpitis. | 2015 | 26187422 |
| microbial diversity in failed endodontic root-filled teeth. | persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment. this study used 16s rrna sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment. | 2012 | 22613548 |
| molecular analysis of 16s rrna genes identifies potentially periodontal pathogenic bacteria and archaea in the plaque of partially erupted third molars. | small subunit rrna sequencing and phylogenetic analysis were used to identify cultivable and uncultivable microorganisms present in the dental plaque of symptomatic and asymptomatic partially erupted third molars to determine the prevalence of putative periodontal pathogens in pericoronal sites. | 2012 | 22326171 |
| brain abscess potentially resulting from odontogenic focus: report of three cases and a literature review. | odontogenic foci can rarely cause intracranial infection. hematogenous spread is considered to be the most important pathophysiological mechanism of intracranial infection of odontogenic origin. to investigate the oral origin of intracranial infections, oral surgeons should understand the underlying mechanisms by which oral bacteria spread to the central nervous system. however, there have been very few reports of intracranial infection resulting from odontogenic infection. | 2017 | 28286386 |
| profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws. | microbial etiology for anti-osteoclastic drug-related osteonecrosis of the jaw (aronj) was suggested. this study investigates any link between bacteria colonizing aronj sites and other oral cavity sites. microbiota samples of 10 aronj patients were collected from the exposed bone, adjacent teeth, contralateral teeth, and tongue. dna checkerboard hybridization was used for microbiota analysis with 43 genomic dna probes prepared from human oral bacterial (38) and candida (5) species, using socrans ... | 2016 | 27419922 |
| assessment of periodontopathogens in subgingival biofilm of banded and bonded molars in early phase of fixed orthodontic treatment. | to assess the prevalence and occurrence of eleven periodontopathogens in subgingival biofilm of banded and bonded molars during the first period of fixed orthodontic treatment. subjects were selected from patients referred to orthodontic treatment and were divided in two groups: group a comprised fifteen patients (14.4±2.45 years of age) who received orthodontic bands on first permanent molars and group b of ten patients (15.7±1.87 years of age) with directly bonded tubes on the labial surface o ... | 2016 | 27020873 |
| an endodontic-periodontal lesion with primary periodontal disease: a case report on its bacterial profile. | the microflora in the periodontal pockets can affect the dental pulp and cause endodontic-periodontal lesions or retrograde pulpitis. here we report an endodontic-periodontal lesion together with its bacterial profile. the lesion occurred in the maxillary right first molar of a 40-year-old woman who presented at our hospital complaining of a violent toothache since the previous night. clinically, the tooth was caries-free and an electric pulp test showed it to be vital. the tooth showed signs of ... | 2014 | 24717928 |
| the effects of stress hormones on growth of selected periodontitis related bacteria. | the focus of this study was to examine in vitro the effects of stress hormones (catecholamines: epinephrine, norepinephrine, dopamine and hydrocortisone: cortisol) on the growth of four anaerobic species of periodontitis-related bacteria (fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and tannerella forsythia) and one facultative anaerobic species (eikenella corrodens). bacterial growth was determined by two different methods: fluorescence in situ hybridization (fish), ... | 2013 | 24036419 |
| subgingival biodiversity in subjects with uncontrolled type-2 diabetes and chronic periodontitis. | there is a bidirectional relationship between periodontal disease and type-2 diabetes mellitus (dm). inflammatory mediators may negatively affect glycemic control, and increased glucose levels and resultant glycation end-products may alter the host response against bacterial infection. however, no agreement has been reached regarding the effect of dm on periodontal subgingival microbiota. therefore, the purpose of the present study was to compare the subgingival biodiversity in deep periodontal ... | 2013 | 22762355 |
| anaerobic and microaerophilic pathogens in the biofilm formation on voice prostheses: a pilot study. | voice rehabilitation with voice prostheses is a standard therapy in laryngectomized patients. biofilm formation on the surface of the voice prostheses causes device failure and requires frequent replacements. studies analyzing the biofilm of voice prostheses have mainly focused on aerobic bacteria. anaerobic bacteria as an integral part of the biofilms on voice prostheses have not been investigated yet. | 2012 | 22374743 |
| isolation of alkaline-tolerant bacteria from primary infected root canals. | alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. the aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (s1), after chemomechanical root canal preparation (s2), and after calcium hydroxide dressing (s3). | 2015 | 25638530 |
| murine experimental root canal infection: cytokine expression in response to f. nucleatum and e. faecalis. | the aim of this study was to evaluate the gene expression of proinflammatory (rankl, tnf-a and ifn-g) and regulatory (tgf-b and il-10) cytokines as reaction to experimental infection by mono or bi-association of fusobacterium nucleatum (atcc 10953) and enterococcus faecalis (atcc 19433). f. nucleatum and e. faecalis, either in mono- or bi-association were inoculated into the root canal system (rcs) of balb/c mice. animals were sacrificed at 10 and 20 days after infection and periapical tissues s ... | 2016 | 27982237 |
| rose bengal uptake by e. faecalis and f. nucleatum and light-mediated antibacterial activity measured by flow cytometry. | antibacterial photodynamic therapy (apdt) using rose bengal (rb) and blue-light kills bacteria through the production of reactive oxygen derivates. however, the interaction mechanism of rb with bacterial cells remains unclear. this study investigated the uptake efficiency and the antibacterial activity of blue light-activated rb against enterococcus faecalis and fusobacterium nucleatum. spectrophotometry and epifluorescence microscopy were used to evaluate binding of rb to bacteria. the antibact ... | 2016 | 27394008 |
| bimix antimicrobial scaffolds for regenerative endodontics. | eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. the aim of this study was to synthesize and determine in vitro both the antimicrobial effectiveness and cytocompatibility of bimix antibiotic-containing polydioxanone-based polymer scaffolds. | 2014 | 25201643 |
| the effect of sodium hypochlorite on enterococcus faecalis when grown on dentine as a single- and multi-species biofilm. | enterococcus faecalis is often involved in the aetiology of apical periodontitis after endodontic treatment. this project aimed to establish, on dentine in vitro, a multi-species biofilm containing e. faecalis, and to determine if the organism had an increased resistance to sodium hypochlorite compared with an axenic biofilm. biofilms were established on dentine discs in flow cells with either e. faecalis alone (axenic) or together with fusobacterium nucleatum and streptococcus sanguinis. follow ... | 2014 | 25195495 |
| susceptibility of methacrylate-based root canal filling to degradation by bacteria found in endodontic infections. | to present a case of endodontic failure obturated with a methacrylate-based root filling material, resilon/realseal (rs). to determine if rs is susceptible to biodegradation by endodontically relevant microbes by a method known to show rs degradation. | 2014 | 25019109 |
| [evaluation on antimicrobial efficacy of fufang bingpeng irrigant: an in vitro study]. | to evaluate the antimicrobial efficacy of fufang bingpeng irrigant and bingpeng irrigant on four endodontic pathogen bacteria. | 2013 | 23991570 |
| antibacterial effect of different root canal sealers on three bacterial species. | bacteria persisting in the root canal system may thwart endodontic therapy. it is therefore interesting to know whether clinically available root canal sealers have an antimicrobial effect. the objective of the present in vitro study was to investigate the antibacterial effect of various sealers on the endodontologically detectable species enterococcus faecalis (e. faecalis), fusobacterium nucleatum (f. nucleatum) and porphyromonas gingivalis (p. gingivalis). | 2013 | 23523285 |
| efficacy of berberine, an antimicrobial plant alkaloid, as an endodontic irrigant against a mixed-culture biofilm in an in vitro tooth model. | berberine, a plant alkaloid isolated from many medicinal plants, has shown antimicrobial activity against selected oral pathogens. the purpose of this investigation was to evaluate the antimicrobial efficacy of berberine solution against selected endodontic pathogens using a multispecies biofilm tooth model. | 2012 | 22794217 |
| characterization of microbiota of root canal-treated teeth with posttreatment disease. | this study evaluated the microbiota of root canals undergoing retreatment. the most prevalent taxa detected by checkerboard included propionibacterium species, fusobacterium nucleatum, streptococci, and pseudoramibacter alactolyticus. quantitative real-time pcr detected enterococcus faecalis and streptococci in 38% and 41% of the cases, comprising 9.76% and 65.78% of the total bacterial counts, respectively. the findings call into question the status of e. faecalis as the main pathogen and sugge ... | 2012 | 22403423 |
| discovery and characterization of de novo sialic acid biosynthesis in the phylum fusobacterium. | sialic acids are nine-carbon backbone carbohydrates found in prominent outermost positions of glycosylated molecules in mammals. mimicry of sialic acid (n-acetylneuraminic acid, neu5ac) enables some pathogenic bacteria to evade host defenses. fusobacterium nucleatum is a ubiquitous oral bacterium also linked with invasive infections throughout the body. we employed multidisciplinary approaches to test predictions that f. nucleatum engages in de novo synthesis of sialic acids. here we show that f ... | 2016 | 27613803 |
| fad-i, a fusobacterium nucleatum cell wall-associated diacylated lipoprotein that mediates human beta defensin 2 induction through toll-like receptor-1/2 (tlr-1/2) and tlr-2/6. | we previously identified a cell wall-associated protein from fusobacterium nucleatum, a gram-negative bacterium of the oral cavity, that induces human beta defensin 2 (hbd-2) in primary human oral epithelial cells (hoecs) and designated it fad-i (fusobacterium-associated defensin inducer). here, we report differential induction of hbd-2 by different strains of f. nucleatum; atcc 25586 and atcc 23726 induce significantly more hbd-2 mrna than atcc 10953. heterologous expression of plasmid-borne fa ... | 2016 | 26930710 |
| peripheral blood neutrophil cytokine hyper-reactivity in chronic periodontitis. | pro-inflammatory cytokine release (il-8, il-6, tnf-α, il-1β) by peripheral blood neutrophils, isolated from periodontitis patients (before/after therapy) and matched controls, was determined after 18 h culture in the presence/absence of escherichia coli lps, opsonised staphylococcus aureus, heat-killed fusobacterium nucleatum and porphyromonas gingivalis. all cultures demonstrated differences in the amounts of each cytokine detected (p < 0.0001), with a clear release pattern (il-8 > il-6 > tnf-α ... | 2015 | 26055820 |
| fusobacterium and escherichia: models of colorectal cancer driven by microbiota and the utility of microbiota in colorectal cancer screening. | intestinal microbiota has emerging roles in the development of colorectal cancer (crc). intestinal dysbiosis, with altered levels of specific bacteria, is consistently seen in crc. the heart of the debate lies in whether these bacteria are a cause or consequence of crc. two bacteria in particular, fusobacterium nucleatum and escherichia coli, have consistently been associated with crc. this review will examine evidence supporting oncogenic roles of f. nucleatum and e. coli. the proposed mechanis ... | 2015 | 25582922 |
| fusobacterium and enterobacteriaceae: important players for crc? | the gut microbiota plays an essential role in regulating intestinal homeostasis through its capacity to modulate various biological activities ranging from barrier, immunity and metabolic function. not surprisingly, microbial dysbiosis is associated with numerous intestinal disorders including inflammatory bowel diseases (ibd) and colorectal cancer (crc). in this piece, we will review recent evidence that gut microbial dysbiosis can influence intestinal disease, including colitis and crc. we wil ... | 2014 | 24972311 |
| intestinal dysbiosis: novel mechanisms by which gut microbes trigger and prevent disease. | new research has identified specific intestinal colonizing microbes that can have significant influence on health and disease. evidence is reviewed supporting an association between fusobacterium nucleatum and colon cancer and for a protective role of faecalibacterium prausnitzii in inflammatory bowel disease, of escherichia coli nissle 1917 in acute intestinal inflammation, of bifidobacterium infantis in neonatal necrotizing enterocolitis, and of akkermansia muciniphila in obesity and diabetes. ... | 2014 | 24857830 |
| dna-based adaptive immunity protect host from infection-associated periodontal bone resorption via recognition of porphyromonas gingivalis virulence component. | porphyromonas gingivalis (pg) is one of a constellation of oral organisms associated with human chronic periodontitis. while adaptive immunity to periodontal pathogen proteins has been investigated and is an important component of periodontal bone resorption, the effect of periodontal pathogen dna in eliciting systemic and mucosal antibody and modulating immune responses has not been investigated. | 2014 | 24051159 |
| oxygen deprivation affects the antimicrobial action of ll-37 as determined by microplate real-time kinetic measurements under anaerobic conditions. | some agents, including escherichia coli and group a streptococcus pyogenes cause infections in oxygen depleted sites. ll-37 is a human host defence peptide shown previously to play an important role in controlling infections caused by these bacteria. however, the effect of oxygen levels on the antimicrobial activity of ll-37 remains obscure. in order to test the effect of oxygen (or lack thereof) on ll-37's activity against e. coli and s. pyogenes, a method for adapting commonly used microtiter ... | 2013 | 23664904 |
| the lipid bilayer-inserted membrane protein bama of escherichia coli facilitates insertion and folding of outer membrane protein a from its complex with skp. | folding of β-barrel membrane proteins, either from a urea-unfolded form or from chaperone-bound aqueous forms, has been characterized for pure lipid bilayers. the impact of preinserted integral proteins from biomembranes has not been examined in biophysical comparisons, but this knowledge is important for the characterization of protein assembly machinery in membranes to distinguish specific effects from unspecific effects. here, folding was studied for a β-barrel membrane protein, outer membran ... | 2013 | 23641708 |
| comparative microbial analysis of paired amniotic fluid and cord blood from pregnancies complicated by preterm birth and early-onset neonatal sepsis. | 16s rrna-based genomic analyses have revolutionized our understanding of infectious diseases. many cases which were recognized as "idiopathic" are now known to have an infectious etiology. here, we present a proof-of-concept study to examine the microbial link between intra-amniotic infection (iai) and early-onset neonatal sepsis (eons). | 2013 | 23437088 |
| folding and stability of outer membrane protein a (ompa) from escherichia coli in an amphipathic polymer, amphipol a8-35. | amphipols are a class of amphipathic polymers designed to maintain membrane proteins in aqueous solutions in the absence of detergents. denatured β-barrel membrane proteins, like outer membrane proteins ompa from escherichia coli and foma from fusobacterium nucleatum, can be folded by dilution of the denaturant urea in the presence of amphipol a8-35. here, the folding kinetics and stability of ompa in a8-35 have been investigated. folding is well described by two parallel first-order processes, ... | 2013 | 23370791 |
| porphyromonas gingivalis mediates inflammasome repression in polymicrobial cultures through a novel mechanism involving reduced endocytosis. | the interleukin (il)-1β-processing inflammasome has recently been identified as a target for pathogenic evasion of the inflammatory response by a number of bacteria and viruses. we postulated that the periodontal pathogen, porphyromonas gingivalis may suppress the inflammasome as a mechanism for its low immunogenicity and pathogenic synergy with other, more highly immunogenic periodontal bacteria. our results show that p. gingivalis lacks signaling capability for the activation of the inflammaso ... | 2012 | 22843689 |
| neutrophil superoxide production in the presence of cigarette smoke extract, nicotine and cotinine. | to determine the effect of cigarette smoke extract, nicotine and cotinine on lucigenin-detectable neutrophil superoxide production. | 2012 | 22607095 |
| influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes. | this study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (dm) and generalized chronic periodontitis (chp). | 2017 | 28198980 |
| evaluation of bacteriological profile in the apical root segment of the patients with primary apical periodontitis. | apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. the reason for this disparity might be the technique used for collecting the samples from the pulp canals. hence, we carried out the present study to evaluate the microbial flora in the apical part ... | 2017 | 28050984 |
| hydrogen sulfide production from subgingival plaque samples. | periodontitis is a polymicrobial anaerobe infection. little is known about the dysbiotic microbiota and the role of bacterial metabolites in the disease process. it is suggested that the production of certain waste products in the proteolytic metabolism may work as markers for disease severity. hydrogen sulfide (h2s) is a gas produced by degradation of proteins in the subgingival pocket. it is highly toxic and believed to have pro-inflammatory properties. we aimed to study h2s production from su ... | 2015 | 25280920 |
| an investigation of the presence of specific anaerobic species in necrotic primary teeth. | different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. the aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (pcs) and root canals (rcs) of infected primary teeth using pcr methods. paired pc and rc samples were collected from 15 subjects and analyzed by pcr for the presence of filifactor alocis, fusobacterium nucleatum, parvimonas micra, porphyromonas endod ... | 2013 | 23538426 |