| characterization of a 75-kda epstein-barr virus capsid protein using a new monoclonal antibody h250. | a monoclonal antibody (mab) designated h250, directed against an epstein-barr virus (ebv) capsid antigen, was obtained following immunization of balb/c mice with naked particles from the producer cell line b95.8. this antigen was present in the producer lines b95.8, p3hr1, m81, ri and ca, and absent from the non-producer lines bjab, raji and 1022. h250 did not inhibit the transformation of cord blood lymphocytes by the b95.8 virus, nor did it inhibit ea induction on raji cells by the p3hr1 virus ... | 1989 | 2559442 |
| use of intravenous immunoglobulins in epstein-barr virus induced thrombocytopenia. | | 1989 | 2559477 |
| [the effect of cytomegalovirus and epstein-barr virus infection on immunologic signs]. | patients with active cmv or ebv infection characterized by the presence of specific serum igm and/or high immunofluorescence in igg, have higher levels of circulating immune complexes and a lower phagocytic activity of leucocytes than patients with latent cmv/ebv infections or not infected patients. in active infections at the same time the absolute number of lymphocytes with the surface sign cd8 increases. the amount of lymphocytes with differential antigens cd3 and cd4 does not change substant ... | 1989 | 2559543 |
| cell-mediated immunity in infectious mononucleosis. | | 1989 | 2559613 |
| cd4 as the receptor for retroviruses of the htlv family: immunopathogenetic implications. | | 1989 | 2559614 |
| epstein-barr-virus-related post-bone-marrow-transplant lymphoproliferative disease: association with cytomegalovirus infection and down syndrome donor marrow. | we describe the development of epstein-barr-virus (ebv)-related lymphoproliferative disease (lpd) in the recipient of a histocompatible bone marrow transplant (bmt). although this rare complication is more common in recipients of mismatched bone marrow, several distinguishing features of our case may have contributed to the development of lpd in the recipient of a matched bone marrow transplant. the patient had received marrow from a sibling with trisomy 21, a syndrome associated with variable c ... | 1989 | 2559622 |
| [papular acrodermatitis associated with initial epstein-barr virus infection]. | | 1989 | 2559638 |
| persistent high fever and gall-bladder wall thickening in a child with primary epstein-barr viral infection. | a 3.5 year old girl presented with a history of high fever, rigors, and mild cough for 1 week. physical examination revealed normal chest findings but gross hepatomegaly was detected. liver function tests were abnormal and indicated biliary obstruction. ultrasonography revealed a distended gall-bladder with increased wall thickness up to 0.6 cm. the diagnosis of primary epstein-barr viral infection was eventually made by specific serological study. the patient's fever subsided 2 weeks later and ... | 1989 | 2559686 |
| influence of epstein-barr virus infection on b lymphocyte responses in patients with rheumatoid arthritis. | the influence of epstein-barr virus (ebv) infection on clinical and serological features of rheumatoid arthritis (ra) were studied. patients with in-vivo activated, in-vitro spontaneously proliferating ebv-infected b lymphocytes had higher levels of serum igg and iga, and tended to have more extensive disease. the finding of in-vivo activated ebv-transformed b cells was not specific for ra but was also seen in patients with ankylosing spondylitis. when supernatants of spontaneously proliferating ... | 1989 | 2559741 |
| [optic neuropathy in purtilo's syndrome]. | | 1989 | 2559817 |
| sites of epstein-barr virus replication in acute and chronic active epstein-barr virus infections. | we examined epstein-barr virus (ebv) in saliva samples and peripheral blood mononuclear cells (pbmc) from 9 patients with acute infectious mononucleosis (im) and 4 patients with chronic active ebv infection (cebv). all saliva samples from patients with acute im were positive for ebv by both southern blot hybridization assay and the cord transformation assay. in contrast, ebv dna could not be detected in saliva samples from patients with cebv. only one of the saliva samples from 4 patients with c ... | 1989 | 2559902 |
| generation and characterization of a human monoclonal antibody against phenolic glycolipid-i of m. leprae. | the development of an epstein-barr virus transformed human b-cell line secreting a monoclonal antibody (moab), kr2/b5 is described. kr2/b5 is an igm type of antibody and is highly specific for phenolic glycolipid-i (pgl-i) a component unique to m. leprae. the moab appears to be directed against the terminal sugar residue of the immunodominant trisaccharide component of pgl-i. | 1989 | 2559933 |
| improved sensitivity of detection by avidin-biotin complex (abc) immunocytochemistry in epstein-barr virus serology. | serum antibodies against epstein-barr virus (ebv)-determined antigens have traditionally been titrated by the indirect immunofluorescence (iif) technique. the avidin-biotin complex (abc) immunocytochemical technique was used to determine the serum levels of iga against ebv viral capsid antigen (iga/vca) and iga against ebv early antigen (iga/ea) in sera of 106 nasopharyngeal carcinoma (npc) patients prior to treatment and 100 normal individuals. the sensitivity of the abc technique is enhanced b ... | 1989 | 2559955 |
| fine structure of ebv-infected keratinocytes in oral hairy leukoplakia. | we evaluated biopsy specimens of 42 cases of clinically suspected oral hairy leukoplakia for the pattern and frequency of ultrastructural alterations specific to epithelial cells infected with epstein-barr virus. some structures could clearly be identified as epstein-barr virus at different stages of assembly, but other intranuclear and cytoplasmic alterations were not conclusively identifiable as any known structure. keratinocytes producing epstein-barr virus contained intranuclear particles of ... | 1989 | 2559979 |
| production of carbohydrate-specific human monoclonal antibodies in vitro. | | 1989 | 2560123 |
| effect of alterations of the atg translation start codon of the aprt gene. | | 1989 | 2560336 |
| modification of hla expression as a possible factor in the pathogenesis of burkitt's lymphoma. | our data show that: most bl cells express significantly less hla class i and ii than the corresponding lcls. lymphoblastoid cell lines have a much higher hla class i and ii density than normal peripheral blood b cells. there are qualitative differences in class i and ii expression in most bl/lcl pairs. these qualitative differences are not caused by ebv infection. they might be due to a selective downregulation of hla specifities by c-myc activation. we conclude that these modifications of hla e ... | 1989 | 2560446 |
| epstein-barr virus associated lymphoproliferative diseases (b cell lymphoma) after transplantation. | we report 12 cases of lymphomas which occurred among 1670 patients with kidney or combined renal and pancreatic transplantation. group 1 comprised nine patients presenting with the diffuse form of the disease where immunoblasts or mature plasma cells massively infiltrated all organs. the first symptom was a viral syndrome, associated with a restriction of heterogeneity of immunoglobulins; oligoclonal to monoclonal peaks of immunoglobulins appeared about 50 days after transplantation. all patient ... | 1989 | 2560533 |
| defective immune response to epstein-barr virus in patients with acute lymphocytic leukemia. | thirteen children with acute lymphocytic leukemia (all) receiving maintenance chemotherapy were tested for anti-epstein-barr virus (ebv) antibodies, ebv specific cytotoxic t lymphocyte (ebv-ctl) activity and spontaneous cytotoxicity against raji cells in order to define the defective cellular immunity in all children during complete remission. among 10 seropositive patients, anti-virus capsid antigen (vca) antibody titer varied from 1:20 to 1:320 and anti-ebv nuclear antigen (ebna) antibody was ... | 1989 | 2560606 |
| persistent active herpes virus infection associated with atypical polyclonal lymphoproliferation (apl) and malignant lymphoma. | this study focuses on lymphoproliferative diseases associated with persistent infection by epstein-barr virus (ebv) and human herpes virus 6 (hhv-6). a suggestive premalignant lymphoproliferative state is distinguished from malignant lymphoma and identified as "atypical polyclonal lymphoproliferation" (apl). sixteen cases of herpes virus (hhv)-associated apl are compared with 21 cases of hhv-associated malignant lymphomas (ml), with 108 cases of ebv or hhv-6 related acute infections mononucleosi ... | 1989 | 2560617 |
| [cancer of the rhinopharynx (clinical picture, scanning and statistics)]. | | 1989 | 2560666 |
| secondary lymphomas: a review on lymphoproliferative diseases arising in immunocompromised hosts: prevalence, clinical features and pathogenetic mechanisms. | this paper reviews the major information on lymphoproliferative diseases developing in primary and acquired immunodeficiencies, in organ allograft recipients, and in different diseases with immune impairment such as rheumatoid arthritis, angioimmunoblastic lymphadenopathy, and hodgkin's disease (secondary lymphomas). the hypothetical role of epstein-barr virus (ebv) in the pathogenesis of lymphoproliferative diseases in immunocompromised hosts has come from the examination of lymphoma cells or t ... | 1989 | 2560763 |
| on the origin of antibodies to immunoglobulin genetic markers in rheumatoid arthritis. an outline of a novel concept of the nature of rheumatoid arthritis--transduction in man. | anti-gm's in rheumatoid arthritis patients detect products of the mendelian genes g1ma, g1mx, g3mb, g3mc, g3mg, g3mst. commonly, these anti-gm's are specific for other individuals immunoglobulin allotypes. reasons are given for the contention that such anti-gm's in patients with rheumatoid arthritis are indicative of the expression of nonnominal allotypes, the genes for which have been transferred from one individual to another by means of a b cell virus. this process - akin to transduction and ... | 1989 | 2560934 |
| [effect of eb virus on the pathogenesis of primary sjogren's syndrome]. | | 1989 | 2560944 |
| [mapping and selective expression of nuclear antigens ebna 3, 4 and 6 of epstein-barr virus]. | | 1989 | 2561013 |
| hairy leukoplakia, ebv: an oral history of aids. | | 1989 | 2561057 |
| x-linked lymphoproliferative syndrome. | the x-linked lymphoproliferative (xlp) syndrome is characterized by a selective immunodeficiency to epstein-barr virus (ebv) manifested by severe or fatal infectious mononucleosis and acquired immunodeficiency. prospective studies in males prior to ebv infection have demonstrate vigorous cytotoxic cellular responses, which are predominantly polyclonally activated alloreactive cytotoxic t cells. cytotoxic t cells that recognize ebv-infected autologous b cells have been demonstrated. fatal ebv inf ... | 1989 | 2561059 |
| recent advances on epstein barr virus and associated diseases. | | 1989 | 2561169 |
| immunotherapy and enhanced antibody-dependent cell-mediated cytotoxicity using virally-infected target cells. | we examined the ability of in vitro addition of interleukin-2 (il-2) to differentially enhance antibody-dependent cell mediated cytotoxicity (adcc) utilizing cultured epstein-barr virus infected cells and gammaglobulin (sandoglobulin). we found significant enhancement of adcc when il-2 was added. chronic epstein-barr virus or chronic fatigue syndrome patients in a therapeutic gammaglobulin program may benefit from il-2 given in vivo. | 1989 | 2561291 |
| [expression of the epstein-barr virus p150 viral capsid antigen in escherichia coli for the use as antigen in diagnostic tests]. | we have attempted to produce the p150 viral capsid antigen of epstein-barr virus (ebv) in escherichia coli. this protein was found, by immunoprecipitation, to be a clinically relevant antigen, especially for the determination of the iga-titer in patients with naso pharyngeal carcinoma (npc). since the expression of the entire p150 coding region was unsuccessful, we synthesized only the antigenic parts of this protein. after purification of p150 expressed by pur290cxh580 with column chromatograph ... | 1989 | 2561513 |
| seroepidemiological study of epstein-barr virus infection in children in taipei. | serumigg antibody titer against viral capsid antigen(vca) of epstein-barr virus (ebv) was measured by indirect immunofluorescence antibody method in 1350 children under 15 years of age, 723 males and 627 females, who were living in the taipei area. sera were collected from june through october 1984. under the age of 6 months, the prevalence rate of positive serum vca antibody was 38.4%. it decreased to 6.4% between 6 and 12 months of age, but rose to 65.8% during the second year of life. the rat ... | 1989 | 2561579 |
| neuromyelitis optica (devic's disease) report of one case. | neuromyelitis optica also named devic's disease is an acute combined optic neuritis and transverse myelitis. it is thought to be a variant of multiple sclerosis, but its clinical presentation probably has only one attack without further recurrence and exacerbation. we present a 12-year-old girl who suffered from sudden onset of lower extremeties weakness, sensory loss and blurred vision after a prodromal uri symptom. csf examination showed mild pleocytosis, elevated immunoglobulins, mild elevati ... | 1989 | 2561581 |
| reactivation of antibody genes in epstein-barr virus transformed human b cell lines. a preliminary report. | three immortalised human b cell lines, which had either last their capacity to secrete specific antibody or secreted low levels of antibody were studied in an attempt to reactivate or enhance antibody synthesis. a variety of different stimuli known to cause activation, proliferation and differentiation of normal b cells were used including polyclonal activators and recombinant interferon. four of them, (namely lps, anti-igm, il2 and il6) effectively increased specific antibody synthesis after th ... | 1989 | 2561727 |
| sjögren's syndrome. | | 1989 | 2561729 |
| [cluster headache, epstein-barr and burkitt's lymphoma virus infections]. | thirteen patients affected by burkitt's lymphoma were studied. six out of them had cluster headache associated with burkitt's lymphoma, and in this group we observed the absence of hla-b14 antigen. the presence of hla-b14 antigen in the remaining seven patients, confirm the hypothesis of a protective role of this antigen against cluster headache. | 1989 | 2561749 |
| [bilateral white lesions of the tongue. description of a case]. | | 1989 | 2561885 |
| epstein-barr virus-associated hemophagocytic syndrome: clinical presentation and treatment. | | 1989 | 2561995 |
| a kinetic analysis of lymphocyte 5'-nucleotidase in b-cell clones from control subjects and patients with primary hypogammaglobulinaemia. | the affinity of lymphocyte 5'-nucleotidase (5nt) for its substrate adenosine monophosphate (amp) was studied in epstein-barr virus immortalized b-lymphocyte clones from healthy controls and patients with primary hypogammaglobulinaemia. km values for amp for 5nt in most of the patient clones were found to be significantly increased compared to b-cell clones from normal subjects, whereas vmax values were within the normal range. | 1989 | 2561998 |
| mechanisms of b-lymphocyte suppression induced by acute physical exercise. | blood mononuclear cells from 20 healthy untrained volunteers were isolated before, during, 2 hr and 24 hr after bicycle exercise at 80% of vo2 max for 1 hr. a reverse plaque forming cell assay was used to investigate b-lymphocyte function. stimulation with pokeweed mitogen, recombinant interleukin 2 and epstein-barr virus resulted in significant decreases in numbers of igg-, igm- and iga-secreting blood cells during as well as 2 hr after exercise, with reversal to pre-exercise values 24 hr later ... | 1989 | 2562000 |
| [a case of infectious mononucleosis in a 2-month-old infant]. | | 1989 | 2562164 |
| forward and reverse changes in ig/myc translocation carrying tumors. | translocation of the c-myc protooncogene to an immunoglobulin locus is a rate-limiting step in the genesis of three b cell derived tumors: burkitt lymphoma (bl) in humans, mouse plasmacytoma (mpc), and rat immunocytoma (ric). the translocation appears as a rate-limiting step in the genesis of all three tumors. its consequences have been best analyzed in bl. they involve a non-immunological and an immunological component. the former acts by preventing the b cell from leaving the cycling compartme ... | 1989 | 2562178 |
| [uveitis of viral origin]. | most well-defined viral uveitis cases are due to herpes viruses. thus, hsv and vzv may cause a chronic anterior uveitis, usually with other characteristic signs. cytomegalovirus causes a necrotic and haemorrhagic retinitis in immunosuppressed patients. acute retinal necrosis also seems to be caused by members of the herpes virus family. many cases of intraocular inflammation have been described in association with viral diseases, but this does not necessarily imply the presence of virus in the e ... | 1989 | 2562202 |
| immunoglobulin vh gene expression in human b cell lines and tumors: biased vh gene expression in chronic lymphocytic leukemia. | we have studied frequencies of vh gene utilization in a panel of monoclonal epstein-barr virus (ebv)-transformed b cell lines derived from human adult and fetal tissues as well as in monoclonal b cells obtained from fresh chronic lymphocytic leukemia (cll) samples. the results show that igm-secreting ebv cell lines from both fetal and adult tissues utilize vh genes from particular families roughly in proportion to estimated family size, suggesting that the repertoire of sigm-positive b cells in ... | 1989 | 2562243 |
| evidence for differential expression of cd45 isoforms by precursors for memory-dependent and independent cytotoxic responses: human cd8 memory ctlp selectively express cd45ro (uchl1). | on the basis of differential cd45 expression, human t cells can be separated into approximately reciprocal populations. the mab uchl1 detects a 180 kd molecular mass isoform, cd45r0. the cd45ra cluster of mabs reacts with cd45 isoforms of 205 and 220 kd molecular mass. these reagents subdivide both cd4 and cd8 t cell populations. cd4 t cells that proliferate in response to memory-dependent (recall) antigens have been shown to selectively express cd45r0. we extend these observations to a model fo ... | 1989 | 2562244 |
| other virus infections in aids. iii. epstein-barr virus. | | 1989 | 2562250 |
| chronic infectious mononucleosis; a case report. | a 39-year-old woman had an adenitis colli followed by anaemia, splenomegaly, atypical lymphoid cells in blood with increased b-lymphocytes, reduced t-suppressor/cytotoxic cells, increased polyclonal igm, high titres of eb vca igg, eb ea igd&r +/- and ebna igg-. the disease progressed slowly for 2 years, splenectomy was followed by clinical improvement; spleen morphology was compatible with a benign disease of viral origin. | 1989 | 2562252 |
| molecular biology of lymphoid malignancies. | | 1989 | 2562284 |
| [evaluation of serological criteria for the diagnosis of infectious mononucleosis]. | we have evaluated the application for the diagnosis of infectious mononucleosis of several serologic criteria, including measurement of concentrations of igm against viral capsid antigen (vca) of epstein-bar virus (ebv) by elisa, detection of heterophil antibodies and the presence of anti-vca igg in absence of igg against nuclear antigen of ebv (ebna). such criteria were evaluated by using a board of sera obtained from cases who had clinically been diagnosed of infectious mononucleosis and ident ... | 1989 | 2562304 |
| epstein-barr virus and its antigens. | | 1989 | 2562309 |
| [infections in the newborn infant]. | | 1989 | 2562316 |
| a human-mouse hybridoma which secretes monoclonal thyroglobulin autoantibody with properties similar to those of the donor patient's serum autoantibody. | human monoclonal antibodies produced by epstein barr (eb) virus transformation and/or cell fusion are frequently igm antibodies which tend to cross react with a range of antigens and often bear little relationship to the highly specific igg antibodies associated with human autoimmune disease. by fusing eb virus transformed b lymphocytes from a hashimoto patient with a mouse myeloma line and selecting for synthesis of igg class thyroglobulin (tg) antibody, we have developed a hybridoma (vb/5) sec ... | 1989 | 2562381 |
| monoclonal autoantibodies derived from multiple sclerosis patients and control persons and their reactivities with antigens of the central nervous system. | peripheral blood b lymphocytes of multiple sclerosis (ms) patients and control persons were transformed with epstein-barr virus. antibody production of transformed cells against isolated human myelin was investigated by enzyme-linked immunosorbent assay (elisa). cells producing reactive antibodies were cloned and propagated to produce monoclonal antibodies (mabs). these mabs did also react with acetone fixed frozen sections of normal human white matter, as determined by indirect immunofluorescen ... | 1989 | 2562390 |
| role of different lymphocyte subsets in human anti-viral t cell cultures. | we have systematically studied uncloned human cell lines derived from anti-influenza a virus or anti-epstein-barr virus (ebv) bulk cultures, or from cultures highly enriched for cd4+ or cd8+ lymphocytes. the most noteworthy results are the following: (1) anti-viral bulk cultures consisted of more than 90% of cd8+ cells in all cases. in contrast, anti-hla cell lines are composed of approximately 50% cd8+ and 50% cd4+ cells. all of the cd8+ and cd4+ cells present in the culture were also 4b4+/2h4- ... | 1989 | 2562930 |
| human herpesvirus-6 and cross-reactivity with other herpesviruses. | | 1989 | 2563119 |
| genomic organization and polymorphisms of the human c3d/epstein-barr virus receptor. | the human c3d/epstein-barr virus receptor (cr2/cd21) is a 145-kda protein primarily expressed on mature b lymphocytes. cr2 is a member of the regulators of complement activation (rca) gene family found on band q32 of chromosome 1. the rca proteins are characterized by the presence of 60-70 amino acid short consensus repeats (scr). a full length cr2 cdna was cloned and used to identify overlapping cosmid genomic clones. analysis of cr2 exon-intron junctions revealed the presence of three types of ... | 1989 | 2563370 |
| epstein-barr virus in brain and rasmussen's encephalitis. | | 1989 | 2563444 |
| histiocytic medullary reticulosis: a lethal form of primary ebv infection in young children in taiwan. | | 1989 | 2563546 |
| identification of ebv-dna in tumour cells of aids-related lymphomas by in-situ hybridisation. | | 1989 | 2564083 |
| epstein-barr virus infection and thyroid dysfunction. | | 1989 | 2564972 |
| oncogenic viruses and cervical cancer. | | 1989 | 2564994 |
| epstein-barr virus associated b-cell lymphomas in aids and after organ transplantation. | | 1989 | 2566020 |
| interaction of histidine-rich glycoprotein with human t lymphocytes. | histidine-rich glycoprotein (hrgp), a human plasma and platelet protein, interacts with multiple ligands in vitro, including heparin, plasminogen, thrombospondin, and fibrinogen/fibrin. in this study, the binding of hrgp to human t lymphocytes was characterized. the binding was specific, concentration-dependent, saturable, and reversible. scatchard plot analysis revealed two classes of binding sites: the high affinity class had an apparent dissociation constant (kd) of 1.92 x 10(-8) m, with 0.92 ... | 1989 | 2566603 |
| epstein-barr virus silver anniversary. | | 1989 | 2566738 |
| histiocytic medullary reticulosis, a lethal form of primary ebv infection. | | 1989 | 2566806 |
| high frequency of epstein-barr virus genome in hiv-positive patients with hodgkin's disease. | | 1989 | 2567472 |
| biochemical diagnosis of hunter syndrome on epstein-barr virus-transformed lymphoblastoid cell lines. | long-term lymphoblastoid cell lines have been established from a patient with hunter syndrome, from his mother, an obligate heterozygote, and from several control individuals. biochemical analyses show that lymphoblastoid cells represent a suitable biological material for the diagnosis of hemizygous, affected males and for heterozygous females: clonal analysis demonstrates the mosaicism predicted by the lyon hypothesis. | 1989 | 2567642 |
| epstein-barr virus/complement receptor and epithelial cells. | | 1989 | 2567860 |
| epstein-barr virus/complement receptor and epithelial cells. | | 1989 | 2569630 |
| hla-d typing of heterozygotes using restriction fragment length polymorphism in the hla-dq gene region on the basis of standard band patterns derived from hla homozygotes. | genomic dnas from 37 epstein-barr virus-transformed hla-d homozygous cell lines with different dw specificities, dw1-dw23, and dkt2 were digested with four different restriction endonucleases (ecori, psti, taqi, and mspi) and hybridized to dq alpha and dq beta cdna probes. polymorphic patterns of multiple fragments correlating with dw, dq, and dr haplotypes were detected, and the restriction fragment length polymorphism standard pattern specific for each dw, dq, and dr specificity could be defin ... | 1989 | 2570051 |
| gut perforation after infection with herpes simplex virus and epstein-barr virus. | | 1989 | 2570271 |
| detection of a second widespread strain of epstein-barr virus. | the prevalence of epstein-barr virus (ebv) type b, which was previously found mainly in equatorial africa, was investigated with the polymerase chain reaction in a population of healthy adults in memphis, tennessee. ebv was detected in the throat washings of 34 (22%) of 157 randomly selected donors, 14 (41%) of whom had type b virus and 17 (50%) type a; 3 donors (9%) had both strains. 18 additional adults with human immunodeficiency virus (hiv-1) infection and 6 severely immunocompromised childr ... | 1989 | 2571010 |
| viral genomes in lymphomas of patients with sjögren's syndrome. | the recent isolation of a new member of the herpes virus family (human herpes virus-6, hhv-6) from patients with lymphoproliferative diseases prompted us to examine biopsies from six patients with primary sjögren's syndrome (ss) who developed non-hodgkin's lymphoma. five ss patients developed b-cell lymphoma and one developed a t-cell lymphoma based on immunoglobulin and t-cell antigen receptor (tcar) gene rearrangements. in two ss patients with b-cell lymphomas, viral dnas were detected, includ ... | 1989 | 2571338 |
| [mosquito bite allergies terminating as hemophagocytic histiocytosis: report of a case]. | a 21-year-old woman had suffered from repeated vasculitis and panniculitis with fever and chills after following mosquito bites since age 7. these manifestations were confirmed with a direct mosquito bite test during her admission to another hospital at age 17. the patient presented to our hospital with progressive dyspnea, productive cough and intermittent fever for one week. in addition to the bilateral infiltrative lesions on chest roentgenography, hepatosplenomegaly with an abnormal liver fu ... | 1989 | 2571673 |
| detection of x-linked lymphoproliferative disease using molecular and immunovirologic markers. | detection of males affected with the x-linked lymphoproliferative disease (xlp) was sought using immunovirologic and molecular genetic linkage techniques. the study population consisted of 20 males in six families with xlp. | 1989 | 2572169 |
| oral hairy leucoplakia. | | 1989 | 2572910 |
| assignment of an anonymous dna sequence ib/d22 (dxs371) to xp11.1-11.4. | in densely mapped and characterized chromosomes such as the x chromosome there are still regions which have a paucity of markers. one of these areas lies in the xp11 region. using human-rodent somatic cell hybrids we have assigned a newly isolated clone ib/d22 to the xp11.1-p11.4 region. | 1989 | 2573448 |
| characteristics of a human liver allograft--derived t-cell line that exhibits suppressor activity. | in an attempt to identify predominant cell populations that may mediate liver allograft dysfunction, the phenotypic and functional characteristics of lymphoid cells propagated from needle biopsy specimens of rejecting liver transplants were examined. in one case, a t-cell line of host phenotype propagated from a liver allograft biopsy demonstrated significant in vitro suppressor activity. this t-cell line (designated jb) was maintained for almost one year in culture with medium containing human ... | 1989 | 2573593 |
| non-hodgkin lymphoma and carbamazepine. | | 1989 | 2573783 |
| multipoint linkage mapping of the xq25-q26 region in a family affected by the x-linked lymphoproliferative syndrome. | we have performed, in a large swiss family, a study of linkage between various dna markers in the xq24-27 region and the locus for the x-linked lymphoproliferative syndrome (xlp). our results indicated that the marker dxs37 in xq25-q26 is genetically linked to the xlp syndrome. the multipoint linkage analysis showed that the disease locus is distal to dxs11, but proximal to the hypoxanthine phosphoribosyl-transferase gene (hprt). | 1989 | 2574086 |
| expression of the epstein-barr virus (ebv) receptor on the surface of cells infected with ebv derived from nasopharyngeal carcinoma. | an epstein-barr virus (ebv) genome-positive epithelial hybrid cell line, npc-kt, derived from the fusion of primary nasopharyngeal carcinoma cells with a human epithelial cell line of adenoid origin and a subline of ebv genome-positive ramos cells, ramos/npc, converted after infection with npc-kt ebv have been previously described (takimoto et al., 1984; takimoto et al., 1987). the npc-kt cells produce virus (npc virus) with both transforming and lytic properties. in this study, npc-kt and ramos ... | 1989 | 2574937 |
| antibodies to epstein-barr virus-induced early antigens in blood donors. | igg class antibodies to the early antigen complex (ea; d + r components) of the epstein-barr virus (ebv) were found by indirect immunofluorescence in titres greater than or equal to 1:10 in 196 (49.4%) out of 397 blood donors and titres greater than or equal to 1:20 in 84 (21.2%) of these subjects. anti-ea titres of greater than or equal to 1:2560 were detected in 6 sera, anti-ea(d) only in 17 donors (4.3%). additional ebv-specific antibody tests were performed in sera with anti-ea-igg titres of ... | 1989 | 2574941 |
| the epstein-barr virus receptor on two nasopharyngeal carcinoma model cell lines. | it was reported that the okb7 monoclonal antibody to c3d receptor could directly inhibit epstein-barr virus (ebv) attachment to and infection of b-lymphocytes. so we tested whether the okb7 could inhibit superinfection of two epithelial npc model cell lines (d98/hr-1 and npc-kt) with ebv. pretreatment of b-lymphocytes with the okb7 significantly inhibits ebv infection. however, pretreatment with the okb7 had no effect on superinfection of d98/hr-1 and npc-kt cells. these data suggest that an ebv ... | 1989 | 2574946 |
| interleukin 2 production by alloantigen-stimulated cd4+ and cd8+ human t cell subsets: frequency of hla class i or class ii-reactive precursor cells and clonal specificity of activated t cells. | a recently developed limiting dilution (ld) method was used to analyze the frequency and specificity of il2-producing cells within alloantigen-stimulated human cd4+ and cd8+ t cell subsets. cell sorter-separated cd4+ and cd8+ responder cells were cocultured under ld conditions with hla class i and/or class ii different epstein barr virus (ebv)-transformed lymphoblastoid cells line (lcl) stimulator cells in the absence of additional factors. after 3 days, il2 in cell-free culture supernatants was ... | 1989 | 2575597 |
| some immunological characteristics of subjects suffering from frequent herpes simplex virus recrudescences. | some parameters of specific and non-specific immunity were tested in a group of 44 subjects suffering from frequent herpes simplex type 1 (hsv-1) or herpes simplex type 2 (hsv-2) recrudescences. the tests performed included determinations of (i) hsv complement-independent and complement-dependent neutralizing antibodies, (ii) antibodies to glycoprotein c of hsv-1 and glycoprotein g of hsv-2, (iii) antibodies to viral capsid and early antigens of epstein-barr virus, (iv) antibodies to tetanus tox ... | 1989 | 2576584 |
| the human dna tumor viruses: human papilloma virus and epstein-barr virus. | hpv and ebv are common infectious agents that persist after primary infection in a latent state with occasional shedding of virus. therefore, one of the fundamental questions in the etiology of those cancers that are linked to infection with such ubiquitous viruses is why cancer develops in a few people when many are infected. because only a small subset of infected people will develop specific cancers, it has been suggested that the presence of the viral genomes in the malignancies merely indic ... | 1989 | 2577002 |
| leucoagglutinin induces differential proliferation of lymphocyte subsets. | in this study we have established culture conditions that allow the preferential and rapid expansion of either t cell receptor (tcr)+/cd3+16- t lymphocytes or tcr-/cd3-16+ natural killer (nk) cells, or the non-selective outgrowth of both subsets. optimal proliferation of lymphocytes was obtained using a combination of irradiated allogeneic peripheral blood lymphocytes (pbl) and irradiated epstein barr virus (ebv) transformed lymphoblastoid b cell lines (b-lcl). addition of 1 microgram/ml leucoag ... | 1989 | 2577067 |
| a region of the epstein-barr virus nuclear antigen leader protein and adenovirus e1a are identical. | | 1988 | 2577878 |
| evidence for active epstein-barr virus infection in patients with persistent, unexplained illnesses: elevated anti-early antigen antibodies. | forty-four patients, including 26 adults and 18 children under 15 years of age, were referred for evaluation of recurrent or persistent illnesses, with symptoms including pharyngitis, lymphadenopathy, fever, headaches, arthralgia, fatigue, depression, dyslogia, and myalgia. thirty-nine patients were positive for epstein-barr virus antibody with antibody levels compatible with active infection for at least 1 year. antiviral capsid antigen and anti-early antigen titers of patients were significant ... | 1985 | 2578266 |
| persisting illness and fatigue in adults with evidence of epstein-barr virus infection. | clinical, serologic, virologic, and immunologic evaluations for 31 adults with chronic illness and fatigue suggested that 23 had persisting epstein-barr virus infection. among these 23 patients, cellular immune mechanisms were generally normal, but 4 had mild immunoglobulin deficiencies. however, 20 patients had abnormal serologic profiles specific for epstein-barr virus shown by significantly elevated titers of antibodies to the viral capsid antigen or early antigen, or by a deficiency of late- ... | 1985 | 2578268 |
| amphotropic retrovirus vector transfer of the v-ras oncogene to human hematopoietic and stromal cells in continuous bone marrow cultures. | human continuous bone marrow cultures were established from intraoperative marrow specimens and infected with amphotropic murine leukemia virus (am-mulv) pseudotypes of kirsten or harvey murine sarcoma virus, and the biologic effects were compared with mouse continuous bone marrow cultures. cultures were tested for production of total nonadherent granulocytes and granulocyte-macrophage progenitor cells (gm-cfuc); virus replication by supernatant reverse transcriptase activity; percentage of adhe ... | 1985 | 2578839 |
| generation of epstein-barr virus antigen-specific suppressor t cells in vitro. | immunosuppression is a commonly observed phenomenon in epstein-barr virus (ebv)-associated disorders and malignancies. the purpose of this study was to determine whether ebv antigens could generate suppressor cell activity in vitro. peripheral blood lymphocytes (pbl) were first treated with various concentrations of ebv antigens or culture medium for 5 days and then with mitomycin c. the cells were then washed and tested for their ability to abrogate the blastogenic response of fresh, autologous ... | 1985 | 2579037 |
| b5, a new b cell-restricted activation antigen. | the characterization of a new human b cell-restricted activation antigen (b5) is described in this report. with the use of a monoclonal antibody to b5, we show that b5 can be detected on peripheral blood or splenic b cells after 1 day of stimulation with either anti-immunoglobulin, protein a, epstein barr virus, or pokeweed mitogen. in contrast, b5 was not expressed on resting b, t, or myeloid cells. more important, b5 could not be detected on activated t cells or monocytes. the b5 antigen was e ... | 1985 | 2579134 |
| large-scale purification of epstein-barr virus membrane antigen gp340 with a monoclonal antibody immunoabsorbent. | a purification method has been elaborated to isolate epstein-barr (eb) virus membrane antigen, gp340, in milligram amounts. the gp340 was prepared from detergent extracts of b95-8 cells by affinity chromatography with a monoclonal antibody immunoabsorbent. bound material was eluted and the eluate, consisting of 50% gp340, was then fractionated by gel filtration. the final gp340 product was antigenically active and 95% pure. the purification method was found to be rapid and reproducible with no l ... | 1985 | 2579161 |
| immune function in chronic active epstein-barr virus infection. | the spectrum of illness attributed to epstein-barr virus (ebv) includes patients with symptoms persisting for more than 1 year without any other obvious underlying disease. high titers of antibodies to ebv, either igg antiviral capsid antigen or anti-early antigen, can be demonstrated. in this study, 13 patients diagnosed as having chronic active ebv infection were examined to determine aspects of their immunologic status. morphological examination and fluorescent antibody analysis revealed no a ... | 1985 | 2579970 |
| human monoclonal antibodies produced by epstein-barr virus transformed cell lines bind protein a. | epstein-barr virus (ebv) is a polyclonal t-independent activator of viral receptor positive human b lymphocytes. lymphocytes infected in vitro with the virus are transformed into immortalized cell lines [nilsson, k, and klein, g. (1982) adv. cancer res. 37, 319]. in this way human cell lines that secrete specific igm, igg and iga monoclonal antibodies are established. protein a is also a polyclonal t-independent b cell activator [langone, j. j. (1982) adv. immunol. 32, 157], the targets of which ... | 1985 | 2580779 |
| allospecific t cell recognition of hla-a2 antigens: evidence for group-specific and subgroup-specific epitopes. | interleukin 2-dependent alloreactive cytotoxic t cell lines, with activity predominantly directed against the hla-a2 antigen, have been generated in vitro by stimulating blood mononuclear cells from donors nonimmune to the epstein-barr (eb) virus with appropriate numbers of eb virus-transformed b cells from a2-homozygous individuals. such effector cells were tested against a panel of eb virus-transformed target cell lines all expressing the serologically defined a2 antigen but typed into "common ... | 1985 | 2580783 |
| interferon induction in human leukocytes after in vitro exposure to cytomegalovirus or epstein-barr virus. | interferon (ifn) was produced after exposure of human mononuclear leukocytes and bone marrow cells to infectious or noninfectious cytomegalovirus (cmv) in vitro. the ifn was generated mainly by non-b lymphocytes. both alpha- and gamma-type ifn could be demonstrated. cmv antigens were usually not demonstrable in cmv-exposed leukocytes. addition of anti-ifn antibodies did not induce cmv antigens. thus, it seems that the endogenous production of ifn is not responsible for the difficulties in demons ... | 1985 | 2580812 |
| inhibition of epstein-barr virus (ebv) release from the p3hr-1 burkitt's lymphoma cell line by a monoclonal antibody against a 200,000 dalton ebv membrane antigen. | in raising murine hybridoma antibodies against epstein-barr virus (ebv)-induced membrane antigens (ma), we found one antibody that blocked the release of infectious ebv from cultured p3hr-1 cells. this monoclonal antibody (mab) recognized a 200 kd, phosphonoacetic acid-sensitive (late) ma, and did not directly neutralize virus without complement. when this mab was added to 33 degrees c-cultured, spontaneously ebv-producing p3hr-1 cells, the intracellular expression of viral capsid antigen and in ... | 1985 | 2580934 |
| effects of pure interferons on epstein-barr virus infection in vitro. | pure human gamma-interferon as well as alpha-interferon inhibited induction of immunoglobulin synthesis by epstein-barr virus but not by pokeweed mitogen in b lymphocytes from adult but not from newborn humans. the interferons inhibited the infected b lymphocytes directly, irrespective of the epstein-barr virus immune status of the donor, and their inhibitory effect was synergistic. | 1985 | 2580994 |
| production and characterization of a unique monoclonal antibody against human b cells (33.2.1). | a monoclonal antibody specific for a polymorphic antigen on human b cells (33.2.1) was produced and characterized. by flow cytometry, 33.2.1 was found to react with peripheral blood b cells, monocytes, and epstein-barr virus (ebv)-transformed b-cell lines, but not with peripheral blood t cells, mitogen-activated t cells, or allo- or autoactivated t cells. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analysis showed that 33.2.1 recognizes a noncovalently bound bimolecular ... | 1985 | 2581708 |