| [a case of idiopathic portal hypertension complicated with sepsis caused by aeromonas hydrophila]. | a 53 year old man with idiopathic portal hypertension (iph) was admitted because of high fever and diarrhea. nineteen years before admission, he had received splenic hilar renal shunt operation with proximal flush ligation of splenic vein due to gastric varices. three months before admission, he had been admitted to our hospital for evaluation of liver dysfunction. liver biopsy examination had revealed peri-portal fibrosis consistent with iph. aeromonas hydrophila was isolated from blood. althou ... | 2005 | 16119770 |
| plasma proteomic analysis of the acute phase response of rainbow trout (oncorhynchus mykiss) to intraperitoneal inflammation and lps injection. | few acute phase proteins are known in fish and better knowledge of them would provide a basis for more reliable methods to objectively assess fish health and welfare. an acute phase response was induced in rainbow trout (oncorhynchus mykiss, walbaum) by inflammation triggered by intraperitoneal administration of purified aeromonas salmonicida lipopolysaccharide emulsified in freund's incomplete adjuvant (lps/fia) or a commercial oil-based multivalent vaccine. acute phase proteins were characteri ... | 2006 | 16139357 |
| purification and characterization of alpha2-macroglobulin from grass carp ctenopharyngodon idellus: cloning a segment of the corresponding gene. | the plasma protein alpha2-macroglobulin (alpha2m) was purified by gel filtration and anion-exchange chromatography from grass carp plasma. the alpha2m consists of two different subunits of molecular weight 95 kda and 80 kda, respectively. the characteristics of grass carp alpha2m are similar to mammalian alpha2m, in that grass carp alpha2m exists in two forms: a fast-form and a slow-form. the former is complexed with protease. the sequence of grass carp alpha2m-conserved region and a region cont ... | 2006 | 16139520 |
| in vitro susceptibility of antibiotics against vibrio spp. and aeromonas spp. isolated from penaeus monodon hatcheries and ponds. | susceptibility patterns to 16 different antibiotics were investigated against pathogenic vibrio spp. and aeromonas spp. isolated from shrimp culture hatcheries and ponds in india. thirteen species of vibrio (n = 90) and two species of aeromonas (n = 7) isolates were tested by agar disk diffusion. the results show that 100% of the isolates were resistant to ampicillin, and that 43.2% and 47.4% were sensitive to chlortetracycline and erythromycin, respectively. susceptibility patterns of another 1 ... | 2005 | 16139992 |
| kinetic, spectroscopic, and x-ray crystallographic characterization of the functional e151h aminopeptidase from aeromonas proteolytica. | glutamate151 (e151) has been shown to be catalytically essential for the aminopeptidase from vibrio proteolyticus (aap). e151 acts as the general acid/base during the catalytic mechanism of peptide hydrolysis. however, a glutamate residue is not the only residue capable of functioning as a general acid/base during catalysis for dinuclear metallohydrolases. recent crystallographic characterization of the d-aminopeptidase from bacillus subtilis (dppa) revealed a histidine residue that resides in a ... | 2005 | 16142900 |
| new gammaproteobacteria associated with blood-feeding leeches and a broad phylogenetic analysis of leech endosymbionts. | many monophagous animals have coevolutionary relationships with bacteria that provide unavailable nutrients to the host. frequently, these microbial partners are vertically inherited and reside in specialized structures or tissues. here we report three new lineages of bacterial symbionts of blood-feeding leeches, one from the giant amazonian leech, haementeria ghilianii, and two others from placobdelloides species. these hosts each possess a different mycetome or esophageal organ morphology wher ... | 2005 | 16151107 |
| predictive modelling of growth and measurement of enzymatic synthesis and activity by a cocktail of selected enterobacteriaceae and aeromonas hydrophila. | the possibility was examined of developing a predictive model that would predict food spoilage by combining microbial growth (increase in cellular number) with extracellular enzymatic activity of a cocktail of five strains of enterobacteriaceae: escherichia coli, enterobacter agglomerans, klebsiella oxytoca, klebsiella pneumoniae and proteus vulgaris and one aeromonas hydrophila strain. estimations of growth and enzyme activity were made within a three-dimensional matrix of conditions: temperatu ... | 2005 | 16154655 |
| aeromonas culicicola pidiyar et al. 2002 is a later subjective synonym of aeromonas veronii hickman-brenner et al. 1987. | we re-investigated the taxonomic affiliation between aeromonas culicicola pidiyar et al. 2002 and aeromonas veronii hickman brenner et al. 1987, two species that have previously been shown to be closely related on the basis of gyrb and rpod gene sequencing and amplified fragment length polymorphism fingerprinting. from extended biochemical characterization and fatty acid analysis, it was concluded that a. culicicola did not constitute a unique phenotypic group in the genus aeromonas but instead ... | 2005 | 16156118 |
| isolation of vibrio cholerae from neonates admitted to an urban diarrhoeal diseases hospital in bangladesh. | very little is known about vibrio cholerae infection in neonates with diarrhoea. | 2005 | 16156982 |
| passive transfer of maternal antibodies and their existence in eggs, larvae and fry of indian major carp, labeo rohita (ham.). | lack of immune competence in the early stages of life leads to severe mortality in larval stages of different fish species including indian major carp (imc). investigation through indirect enzyme linked immunosorbent assay (elisa) and agglutination test revealed a significant increase in specific serum antibody response in the brood fish of indian major carp, labeo rohita (ham.) following immunisation with a virulent aeromonas hydrophila bacterin 1 month prior to breeding, which was transferred ... | 2006 | 16157486 |
| purification and characterization of the n-terminal domain of exea: a novel atpase involved in the type ii secretion pathway of aeromonas hydrophila. | aeromonas hydrophila secretes a number of degradative enzymes and toxins into the external milieu via the type ii secretory pathway or secreton. exea is an essential component of this system and is necessary for the localization and/or multimerization of the secretin exed. exea contains two sequence motifs characteristic of the walker superfamily of atpases. previous examination of substitution derivatives altered in these motifs suggested that atp binding or hydrolysis is required for exeab com ... | 2005 | 16159770 |
| evolutionary and diagnostic implications of intragenomic heterogeneity in the 16s rrna gene in aeromonas strains. | sequencing 16s rrna genes (ssu) cloned from aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. the ssu copies from aeromonas veronii lmg13695 clustered with sequences from four aeromonas species. these results demonstrate intragenomic heterogeneity of ssu and suggest caution when using ssu to identify aeromonads. | 2005 | 16159790 |
| antibacterial and antifungal lysozyme-type activity in cameraria ohridella pupae. | lysozyme-type antibacterial and antifungal activity in pupae of cameraria ohridella was studied. activity against micrococcus luteus and bacillus megaterium was detected in pupae extract. also antifungal activity from c. ohridella pupae extract directed against saccharomyces cerevisiae strain w 303 was shown. during immunoblotting two bands in pupae extract, with molecular mass of about 15 and 28 kda were recognized by antibodies directed against hewl. after acid electrophoresis followed by bioa ... | 2005 | 16169556 |
| experimental handling stress as infection-facilitating factor for the goldfish ulcerative disease. | experimental handling stress (ehs) was applied to clinically asymptomatic farmed goldfish (carassius auratus l.). ehs affected the gills and skin integrity of the fish and was accompanied by increased levels of plasma glucose, cortisol and interleukin-10 (il-10). ehs application was followed by highly significant enhancement of the rate of infection with a virulent aeromonas salmonicida isolate. cumulative ulceration at the initial phase of the ensuing goldfish ulcerative disease (gud) evidenced ... | 2006 | 16174536 |
| high pressure processing for food safety. | food preservation using high pressure is a promising technique in food industry as it offers numerous opportunities for developing new foods with extended shelf-life, high nutritional value and excellent organoleptic characteristics. high pressure is an alternative to thermal processing. the resistance of microorganisms to pressure varies considerably depending on the pressure range applied, temperature and treatment duration, and type of microorganism. generally, gram-positive bacteria are more ... | 2005 | 16175246 |
| [synthesis of medium-chain-length polyhydroxyalkanoate (mcl pha) in type i pha synthase negative mutant of aeromonas hydrophila]. | aeromonas hydrophila cgmcc 0911 possessing type i polyhydroxyalkanoate (pha) synthase gene (phac) only accumulate copolyesters consisting of 3-hydroxybutyrate (3hb) and 3-hydroxyhexanoate (3hhx), abbreviated as phbhhx, from lauric acid as sole carbon source but not from glucose. the gene encoding type i pha synthase was interrupted by insertion of a chloramphenicol resistance gene (cm). conjugation of suicide plasmid pfh10 transformed a. hydrophila cgmcc 0911 into a recombinant organism with the ... | 2005 | 16176086 |
| the development of a matrix-assisted laser desorption/ionization mass spectrometry-based method for the protein fingerprinting and identification of aeromonas species using whole cells. | this report describes the development of a method to detect the waterborne pathogen aeromonas using matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms). the genus aeromonas is one of several medically significant genera that have gained prominence due to their evolving taxonomy and controversial role in human diseases. in this study, maldi-ms was applied to the characterization of seventeen species of aeromonas. these seventeen species were represented by thirty-two strains, ... | 2006 | 16176841 |
| the type iii secretion system and cytotoxic enterotoxin alter the virulence of aeromonas hydrophila. | many gram-negative bacteria use a type iii secretion system (ttss) to deliver effector proteins into host cells. here we report the characterization of a ttss chromosomal operon from the diarrheal isolate ssu of aeromonas hydrophila. we deleted the gene encoding aeromonas outer membrane protein b (aopb), which is predicted to be involved in the formation of the ttss translocon, from wild-type (wt) a. hydrophila as well as from a previously characterized cytotoxic enterotoxin gene (act)-minus str ... | 2005 | 16177316 |
| recurrent dialysis-associated aeromonas hydrophila peritonitis: reports of two cases and review of the literature. | | 2005 | 16178485 |
| hydrolytic activity of alpha-amylase in anaerobic digested sludge. | hydrolysis is usually considered to be a rate-limiting step in anaerobic digestion. for improving anaerobic solid waste treatments, it is essential to elucidate the mechanism of hydrolysis. in this study, alpha-amylase, one of the hydrolytic enzymes, was investigated for the elucidation of more precise mechanism of hydrolysis. alpha-amylase activity of solid starch-degrading bacteria (sdb) was estimated through batch experiments with several different substrates and with distinction between cell ... | 2005 | 16180437 |
| granulomatous inflammation of salt glands in ducklings (anas platyrhynchos) associated with intralesional gram-negative bacteria. | the "nasal glands" occur in many bird species and are powerful sodium ion-excretory organs. in ducks, they are located in supraorbital bony recesses. granulomatous inflammation of these glands occurs with an incidence of approximately 1% in ducklings (anas platyrhynchos), and is not associated with specific clinical symptoms. we investigated nine glands of eight animals with granulomas by gross pathology and histopathology, and compared results of bacteriology with 20 non-lesioned nasal glands. ... | 2005 | 16191707 |
| [detection of multiresistance aeromonas with tem type resistant genes in patients with cirrhosis]. | to study the status of beta-lactamase produced by multiresistant aeromonas selected from cirrhosis patients to provide reference for treatment and reduce resistance and control spreading. | 2005 | 16201471 |
| arge-encoded n-acetyl-l-ornithine deacetylase from escherichia coli contains a dinuclear metalloactive site. | the catalytic and structural properties of the arge-encoded n-acetyl-l-ornithine deacetylase (arge) from escherichia coli were investigated. on the basis of kinetic and itc (isothermal titration calorimetry) data, zn(ii) binds to arge with kd values that differ by approximately 20 times. moreover, arge exhibits approximately 90% of its full catalytic activity upon addition of one metal ion. therefore, arge behaves similarly to the aminopeptidase from aeromonas proteolytica (aap) in that one meta ... | 2005 | 16201833 |
| population-based epidemiological study of infections caused by carbapenem-resistant pseudomonas aeruginosa in the calgary health region: importance of metallo-beta-lactamase (mbl)-producing strains. | a study was conducted in the calgary health region between may 2002 and april 2004 to define the population-based epidemiological characteristics of infections caused by imipenem-resistant pseudomonas aeruginosa and to explore the clinical outcomes due to metallo- beta -lactamase (mbl)-producing and non-mbl-producing strains. | 2005 | 16206075 |
| numerical analysis of electrophoretic periplasmic protein patterns of aeromonas sp. strains. | a total of 103 strains of aeromonas spp. isolated from clinical and from environmental samples was compared by using sds-page of periplasmic proteins patterns. strains isolated from polish children suffering from gastroenteritis did not appear similar to strains isolated from human living in hong-kong. aeromonas sp. strains did not show a tendency to cluster according to their origin. our results have demonstrated no species-specific periplasmic protein profiles. a significant protein electropho ... | 2005 | 16209101 |
| purification of bovine milk lactoperoxidase and investigation of antibacterial properties at different thiocyanate mediated. | bovine lactoperoxidase (lpo) was purified with amberlite cg 50 h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography from skim milk. the activity of lactoperoxidase was measured by using 2.2-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (abts) as a choromogenic substrate at ph 6.0. purification degree for the purified enzyme was controlled with sds-page and rz value (a412/a280). rz value for the purified lpo was 0.8. km value ... | 2005 | 16212035 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from gluconate and glucose by recombinant aeromonas hydrophila and pseudomonas putida. | aeromonas hydrophila 4ak4 and pseudomonas putida gpp104 were genetically engineered to synthesize poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx) using gluconate and glucose rather than fatty acids. a truncated tesa gene, encoding cytosolic thioesterase i of escherichia coli which catalyzes the conversion of acyl-acp into free fatty acids, was introduced into a. hydrophila 4ak4. when grown in gluconate, the recombinant a. hydrophila 4ak4 synthesized 10% (w/w) phbhhx containing 14% (mol/mo ... | 2005 | 16215853 |
| biosynthesis and characterization of poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) from palm oil products in a wautersia eutropha mutant. | palm kernel oil, palm olein, crude palm oil and palm acid oil were used for the synthesis of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] by a mutant strain of wautersia eutropha (formerly ralstonia eutropha) harboring the aeromonas caviae polyhydroxyalkanoate (pha) synthase gene. palm kernel oil was an excellent carbon source for the production of cell biomass and p(3hb-co-3hhx). about 87% (w/w) of the cell dry weight as p(3hb-co-3hhx) was obtained using 5 g palm kernel oil/l ... | 2005 | 16215858 |
| comparison of elisa and pcr vis-à-vis cultural methods for detecting aeromonas spp. in foods of animal origin. | the present study was conducted to assess the best method of the most commonly used methods for detection of aeromonads in foods of animal origin. with this objective an omp based indirect plate elisa and a duplex-pcr using primers targeting aerolysin gene and 16s rrna gene and yielding amplicons of 252 bp and 599 bp, respectively, were standardized. the standardized protocols and the conventional cultural method were then compared for their respective sensitivities and specificities for detecti ... | 2006 | 16216375 |
| atypical mri findings in canavan disease: a patient with a mild course. | canavan disease is a severe, progressive leukodystrophy with an autosomal recessive inheritance, caused by aspartoacylase (aspa) deficiency. the characteristic mri features include diffuse, symmetrical white matter degeneration in the subcortical areas, with bilateral involvement of the globus pallidus. proton magnetic resonance spectroscopy of the brain shows an increase in the concentration of n-acetylaspartic acid (naa). the altered naa metabolism has been traced to mutations in the gene enco ... | 2005 | 16217711 |
| characterization of aeromonads isolated from the river narmada, india. | thirty isolates of aeromonas species were isolated from river narmada at jabalpur during the period of january 2002-december 2002. diversity of these fresh water isolates were determined by randomly amplified polymorphic dna (rapd) analysis. these environmental isolates were found to be positive for virulence factors, i.e. protease, amylase, lipase and dnase. isolates were also positive for beta-hemolytic activity. all aeromonas species were tested for antibiotic resistance patterns and were fou ... | 2005 | 16217927 |
| influence of high-m alginate on the growth and survival of atlantic cod (gadus morhua l.) and spotted wolffish (anarhichas minor olafsen) fry. | atlantic cod and spotted wolffish fry were fed high-m alginate containing feed for 59 and 55 days, respectively. during this period the fry showed a higher specific growth rate compared to controls. uptake and distribution of alginate was studied by inclusion of the (125)i-labelled molecule in the feed. the stomach and intestine contained the highest amount while the kidney, liver and spleen contained some, indicating that the alginate was taken up by the gut and transported to internal organs. ... | 2006 | 16219475 |
| antibiotic binding to monozinc cpha beta-lactamase from aeromonas hydropila: quantum mechanical/molecular mechanical and density functional theory studies. | the active-site dynamics of apo cpha beta-lactamase from aeromonas hydropila and its complex with a beta-lactam antibiotic molecule (biapenem) are simulated using a quantum mechanical/molecular mechanical (qm/mm) method and density functional theory (dft). the quantum region in the qm/mm simulations, which includes the zn(ii) ion and its ligands, the antibiotic molecule, the catalytic water, and an active-site histidine residue, was treated using the self-consistent charge density functional tig ... | 2005 | 16220984 |
| a comparative immunopathological study of injection site reactions in salmonids following intraperitoneal injection with oil-adjuvanted vaccines. | an adequate understanding of the inflammatory mechanisms following intraperitoneally-injected oil-adjuvanted vaccines in fish is required for the improvement of the quality of vaccines. we report the findings of a study in which the importance of neutrophils, macrophages, lymphocytes and mast cells (mcs) in early inflammatory reactions of atlantic salmon were examined in comparison with those of rainbow trout and grayling. the inflamed areas in the pylorus/pancreas area (injection site) were seq ... | 2006 | 16221512 |
| skin and soft-tissue infections among tsunami survivors in southern thailand. | among 777 patients transferred to 4 hospitals in bangkok from southern thailand after the tsunami of 26 december 2004, there were 515 with skin and soft-tissue infections. the most common organisms isolated were aeromonas species (145 [22.6%] of 641 isolates from 305 patients). most isolates were susceptible to aminoglycosides, third- and fourth-generation cephalosporins, quinolones, and imipenem but were resistant to amoxicillin-clavulanate and first-generation cephalosporins. | 2005 | 16231248 |
| characterisation of atypical aeromonas salmonicida infection in arctic charr salvelinus alpinus and european grayling thymallus thymallus. | cultured stocks of arctic charr salvelinus alpinus and european grayling thymallus thymallus are vulnerable to infection by achromogenic atypical aeromonas salmonicida (aas). in finland, natural stocks of both fish species have to be supported by restocking, and aas infection poses a threat to successful restocking because no preventive means are available. in this study, we analysed aas isolates from arctic charr and european grayling and from other sources genetically, and characterised the si ... | 2005 | 16231637 |
| cloning, nucleotide sequence and expression of the beta-n-acetylglucosaminidase gene from aeromonas sp. no. 10s-24. | the beta-glcnacase gene of aeromonas sp. no. 10s-24 was cloned and sequenced. the gene consisted of 2505 bp encoding 835 amino acid residues. the amino acid sequence of the gene shares sequence homology with beta-glcnacases of vibrio vulnificus (37.4%), serratia marcescens (36.4%), v. harveyi (36.4%), v. parahemolyticus (33.5%), alteromonas sp. strain o-7 (27.0%), and candida albicans (28.6%). the beta-glcnacase gene of aeromonas sp. no. 10s-24 was about 630-fold overexpressed in escherichia col ... | 2000 | 16232720 |
| isolation and characterization of aeromonas sp. b-5 capable of decolorizing various dyes. | aeromonas sp. b-5, which has the ability to decolorize azo dyes, was isolated from soil. aeromonas sp. b-5 completely decolorized 100 mg/l of bordeaux s by reductive cleavage of azo bonds under static conditions in 24 h. though the decolorization of bordeaux s by aeromonas sp. b-5 was suppressed under shaking, rapid decolorization was observed when the culture was changed to static conditions after cultivation with shaking. the indigoid dye, acid blue 74, was decolorized by aeromonas sp. b-5 und ... | 2000 | 16232913 |
| expression of a gene encoding chitinase (pca 8 orf) from aeromonas sp. no. 10s-24 in escherichia coli and enzyme characterization. | a gene encoding chitinase from aeromonas sp. no. 10s-24 was expressed using ptrc99a in escherichia coli jm 105 which yielded a 5-fold higher activity than when puc19 was used. three different truncated enzymes (sa-1, sa-2 and sa-3) were obtained after purification. their isoelectric points were 7.0, 6.9, and 6.7, respectively. the enzymes showed two optimum phs, 4.0 and 7.0, when incubated with ethylene glycol chitin as the substrate, and were stable over a wide ph range (3.0-9.0). the optimum t ... | 2001 | 16233047 |
| the processing of high-molecular-weight xylanase (xyne, 110 kda) from aeromonas caviae me-1 to 60-kda xylanase (xyne60) in escherichia coli and purification and characterization of xyne60. | a xylanase gene (xyne) encoding xyne (110 kda) was cloned from a lambda phage genomic library of aeromonas caviae me-1 which is a multiple-xylanase-producing bacterium. upon nucleotide sequence analysis, we found that xyne comprises 2823 by and encodes a protein of 941 amino acid residues (104,153 da), which was similar to endo-beta-1,4-xylanases which are categorized to glycosyl hydrolase family 10. an escherichia coli transformant that harbored pxed30 carrying xyne produced 110-, 84-, 72-, and ... | 2003 | 16233373 |
| a cytoplasmic xylanase (xynx) of aeromonas caviae me-1 is released from the cytoplasm to the periplasm by osmotic downshock. | aeromonas caviae me-1 is a multiple xylanase-producing gram-negative bacterium which was isolated from the gut contents of a wild silkworm, samia cynthia pryeri. one of the xylanases produced by a. caviae me-1, xynx (38 kda, family 10 xylanase), hydrolyzes xylan to xylobiose and xylotetraose as final degradation products. generally, xylanases are extracellular or cell surface enzymes. however, xynx is not exported to the extracellular fluid by a. caviae me-1 and an escherichia coli transformant ... | 2003 | 16233445 |
| processing of xyne (110-kda) of aeromonas caviae me-1 to 72-kda xylanase in escherichia coli transformant. | an extracellular 72-kda xylanase from an escherichia coli transformant that harbored pxed30 carrying xyne of aeromonas caviae me-1 was purified by extraction following sds-page to electrophoretic homogeneity. the analysis of n-terminal 10 amino acid residues (gly-ala-arg-ala-gln-ala-ala-ala-asp-val) revealed a 72-kda product derived by the cleavage of gly26-gly27 at the n-terminal region of 110 kda xyne. the 72-kda xylanase from a. caviae me-1 was found to have the same sequence as that of the n ... | 2003 | 16233547 |
| aeromonas hydrophila exotoxin induces cytoplasmic vacuolation and cell death in vero cells. | many organisms are able to cause cell vacuolation, but it is unclear if this can be considered a step of apoptosis or necrosis, or a distinct form of cell death. in this study vero cells were used to evaluate the relationship between vacuolation and cell death pattern caused by exotoxins produced by environmental strains of a. hydrophila. cell damage has been evaluated morphologically as well as biochemically. cytotoxic and vacuolating titres were strictly correlated and the vacuolation has to b ... | 2005 | 16240698 |
| immunoreactive antigens of the outer membrane protein of aeromonas hydrophila, isolated from goldfish, carassius auratus (linn.). | aeromonas hydrophila causes disease under stress conditions or in concert with infection by other pathogens in goldfish. sero-diagnostic and/or immunoprophylactic tools against aeromonas infection in goldfish are not available so far. the present study was undertaken to fractionate and characterise the outer membrane proteins (omp) of a. hydrophila and to identify suitable immunoreactive components. a total of 10 fractions were generated from crude omp antigens upon gel permeation and subsequent ... | 2006 | 16243540 |
| the major aeromonas veronii outer membrane protein: gene cloning and sequence analysis. | the gene encoding the major outer membrane protein (omp) from aeromonas veronii, omp38, was cloned and characterized. sequence analysis revealed an open reading frame of 1,047 nucleotides coding for a primary protein of 349 amino acids with a 20-amino-acid signal peptide at the n-terminal and the consensus sequence ala-x-ala (ala-asn-ala) as the signal peptidase i recognition site. the mature protein is composed of 329 amino acids with a calculated molecular mass of 36,327 da. the degree of iden ... | 2005 | 16252131 |
| effect of sex ratio on the immune system of oreochromis mossambicus (peters). | the social environment of fish has a crucial role to play on the immune system and hence on the overall health status. stressors of social origin such as dominance, subordination, and fight for mate have a major impact on the immune system of fish. the present study was designed with the objective of finding the effect of sex ratio of the population on the immune system of oreochromis mossambicus. groups of fish were maintained for 28 days in three different sex ratios i.e., (i) all-male (ii) al ... | 2006 | 16256305 |
| immunostimulatory nature of beta-glucans and baker's yeast in gnotobiotic artemia challenge tests. | the use of new preventive approaches such as immunostimulants to reduce stress and mortalities, to maintain good health of cultured organisms and to stimulate the non-specific defence mechanism, is becoming increasingly important in aquaculture. yet detailed analysis reveals that in most experiments the validity of some conclusions with respect to the benefit of immunostimulation is still doubtful, especially in invertebrates. the use of standardized trials under controlled rearing conditions, c ... | 2006 | 16257233 |
| synthesis and evaluation of new quinazolone derivatives of nalidixic acid as potential antibacterial and antifungal agents. | in continuation of our work on synthesis of biheterocycles carrying the biodynamic heterocyclic systems at position 3, a series of new nalidixic acid derivatives having quinazolones moiety were synthesised to achieve enhanced biological activity and wide spectrum of activity. nalidixic acid was first converted into its acid chloride using thionyl chloride as an acylating agent at laboratory temperature. later it was converted to methyl ester. nalidixoyl chloride formed vigorously reacts with met ... | 2006 | 16260068 |
| modulation of juvenile brook trout (salvelinus fontinalis) cellular immune system after aeromonas salmonicida challenge. | in fish, the first line of defence against infectious microorganisms is based on non-specific cellular immune mechanisms (innate immunity). in this study, we measured the non-specific immune parameters (natural cytotoxic cells (ncc) activity, lymphoproliferation, percentage of phagocytosis and phagocytic activity) in brook trout (salvelinus fontinalis) infected by a virulent strain of aeromonas salmonicida. eight days post-infection, the mortality of infected fish reached 70%. a transient immuno ... | 2006 | 16263179 |
| aerobic bacterial flora of the nasal cavity in gulf of california sea lion (zalophus californianus) pups. | nasal swab samples from clinically healthy california sea lions pups (zalophus californianus) from six different reproductive rookeries in the gulf of california were collected to determine the type and frequency of the representative aerobic bacterial microflora of their nasal mucosa. a total of 114 samples were examined and 100 bacterial isolates were identified and typified by microbiological and biochemical standard tests. fifty four isolates corresponded to gram positive bacteria (54%) and ... | 2005 | 16266850 |
| recognition of individual genes in diverse microorganisms by cycling primed in situ amplification. | cycling primed in situ amplification-fluorescent in situ hybridization (cprins-fish) was developed to recognize individual genes in a single bacterial cell. in cprins, the amplicon was long single-stranded dna and thus retained within the permeabilized microbial cells. fish with a multiply labeled fluorescent probe set enabled significant reduction in nonspecific background while maintaining high fluorescence signals of target bacteria. the ampicillin resistance gene in escherichia coli, chloram ... | 2005 | 16269764 |
| putative exposed aromatic and hydroxyl residues on the surface of the n-terminal domains of chi1 from aeromonas caviae cb101 are essential for chitin binding and hydrolysis. | chitinase chi1 of aeromonas caviae cb101 possesses chitin binding sites at both its n and c termini. four putative exposed residues aligned in a line on the surface of the n-terminal domains of chi1 were found to contribute to the enzyme-chitin binding and hydrolysis via site-directed mutagenesis. also, it was found that chi1 requires the cooperation of the n- and c-terminal domains to bind fully with crystalline and colloidal chitin. | 2005 | 16269803 |
| spectroscopic and thermodynamic characterization of the e151d and e151a altered leucine aminopeptidases from aeromonas proteolytica. | previous kinetic characterization of the glutamate 151 (e151)-substituted forms of the leucine aminopeptidase from aeromonas proteolytica (vibrio proteolyticus; aap) has provided critical evidence that this residue functions as the general acid/base. the close proximity of similar glutamate residues to the bridging water/hydroxide of the dinuclear active sites of metalloenzymes (2.80 and 3.94 angstroms in carboxypeptidase g2 and 3.30 and 3.63 angstroms in aap), suggests it may also be involved i ... | 2005 | 16270998 |
| microbiological evaluation of bottled non-carbonated ("still") water from domestic brands in greece. | the microbiological quality of 1,527 samples of bottled non-carbonated ('still') mineral water, purchased from retail outlets and derived from 10 manufacturing companies in greece, was investigated during the period 1995-2003. applying the membrane filter technique, the aliquots of water samples (250 ml) were analyzed for the presence and enumeration of total coliforms, escherichia coli, enterococcus spp. and pseudomonas aeruginosa. also, aerobic bacteria were counted as heterotrophic plate coun ... | 2006 | 16271413 |
| diversity of denitrifying bacteria isolated from daejeon sewage treatment plant. | the diversity of the denitrifying bacterial populations in daejeon sewage treatment plant was examined using a culture-dependent approach. of the three hundred and seventy six bacterial colonies selected randomly from agar plates, thirty-nine strains that showed denitrifying activity were selected and subjected to further analysis. according to the morphological and biochemical properties, the thirty nine isolates were divided into seven groups. this grouping was supported by an unweighted pair ... | 2005 | 16273028 |
| [synthesis of copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate by aeromonas hydrophila wq and its molecular basis]. | aeromonas hydrophila wq isolated from lake water was found to be able to synthesize polyhydroxyalkanoates (pha) copolymer consisting of 3-hydroxybutyrate (hb) and 3-hydroxyhexanoate (hhx) (phbhhx). lauric acid was found to be the most suitable carbon source for cell growth and phbhhx accumulation. the bacteria accumulated 49% phbhhx containing 6% hhx in terms of cell dry weight when grown on lauric acid for 72 h. 42% phbhhx consisting of 14% hhx was obtained with 5 g/l glucose and 10 g/l lauric ... | 2003 | 16276907 |
| [invasion of aeromonas hydrophila in cells involving signal transduction and cytoskeleton]. | invasion of aeromonas hydrophila strain ah j-1 isolated from diseased fish to cultured hep-2cells monolayer was evaluated by the recovery of gentamincin-resistant (gm') bacteria from triton x-100 cell lysates. the invasive efficiency (ie) could reach to 0.2% at 37 degrees c. in addition, potential signal transduction pathways that precede bacterial internalization were studied by using signal transduction inhibitors. bacterial internalization of ah j-1 involved microfilaments and protein tyrosin ... | 2003 | 16276909 |
| [effects of aeromonas hydrophila biofilm on the drug resistance]. | aeromonas hydrophila (ah) biofilm (bf) model in vitro was established and antibiotic effects of 11 antimicrobials on bf and free-cell(fc) bacteria were tested. after the ah j-1 on the surfaces of the silicon pieces was incubated for 7 days in tsb, the intact bf was formed by detection of agno3 solution staining. the fc bacteria were resistant to penicillin but susceptible to enrofloxacin and fulgram. their minimum bactericidal concentrations (mbcs) were 256 microg/ml, 0.03 microg/ml and 0.25 mic ... | 2003 | 16276926 |
| wound infections in tsunami survivors: a commentary. | survivors of the december 2004 indian ocean tsunami sustained a variety of wound infections, ranging from common pathogens to rarely seen organisms. this article discusses the likely microbiology potentially seen in wound infections with exposure to freshwater, seawater, soil, faecal or other contamination, and attempts to provide an organising framework for choosing empiric antibiotics for such infections. therapy for less frequently encountered clinical entities is also discussed, including te ... | 2005 | 16284684 |
| the immunomodulatory effects of tuftsin on the non-specific immune system of indian major carp, labeo rohita. | the purpose of this study was to determine if injections of different dosages of tuftsin would enhance the immune response and disease resistance against the infections due to the opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of tuftsin in pbs suspension at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l. rohita at 2-week intervals for four times. after ... | 2006 | 16293422 |
| prevalence of aeromonas in different clinical and water samples with special reference to gastroenteritis. | a hospital based study was carried out in the department of microbiology, tribhuvan university teaching hospital, institute of medicine with the aim to initiate the isolation and identification of aeromonas spp. from the stool samples of gastroenteritic patients and to determine the prevalence of aeromonas spp. in other clinical samples and water. altogether 293 samples were investigated that include 172 stool samples, 60 pus/wound swabs, 20 body fluids and 41 water samples. the samples were col ... | 2004 | 16295748 |
| carriage by the housefly (musca domestica) of multiple-antibiotic-resistant bacteria that are potentially pathogenic to humans, in hospital and other urban environments in misurata, libya. | using standard microbiological procedures, bacteria that are potentially pathogenic to humans were isolated from 150 houseflies collected in the libyan city of misurata (50 flies each from the central hospital, streets and abattoir). salmonella spp., yersinia enterocolitica and edwardsiella tarda were isolated from flies collected on the streets and in the abattoir but not from those collected in the hospital. shigella sonnei was detected in just one fly, which was collected in the abattoir. of ... | 2005 | 16297293 |
| structural studies of the core region of aeromonas salmonicida subsp. salmonicida lipopolysaccharide. | the core oligosaccharide structure of the in vivo derived rough phenotype of aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, ce-ms and one- and two-dimensional nmr analyses and established as the following: [carbohydrate: see text] where r=alpha-d-galp-(1-->4)-beta-d-galpnac-(1--> or alpha-d-galp-(1--> (approx. ratio 4:3). comparative ce-ms analysis of a. salmonicida subsp. salmonicida core oligosaccharides from strains a449, 80204-1 and ... | 2006 | 16297894 |
| etiology of childhood diarrhea in zliten, libya. | to determine the etiological agents of diarrhea in children from a small semi-urban city in libya and the association of age, gender, seasonal variation, breast-feeding, source of water for drinking, or antibiotic use with the isolation of enteropathogens and whether such agents are community or hospital acquired. | 2005 | 16311662 |
| high dietary vitamin c affects growth, non-specific immune responses and disease resistance in asian catfish, clarias batrachus. | studies were conducted to determine the immunomodulatory effects of high dietary ascorbic acid (vitamin c) on growth, serum concentration, non-specific immune response and disease resistance of a commercially important asian catfish, clarias batrachus. four practical diets were formulated to contain 0, 500, 1000 and 2000 mg ascorbic acid (aa) equivalent/kg diet, supplied as l-ascorbyl-2-polyphosphate (lapp) and were fed for 2, 4, 6 and 8 weeks. each diet was fed to triplicate groups of catfish w ... | 2005 | 16311902 |
| transcriptome response following administration of a live bacterial vaccine in atlantic salmon (salmo salar). | antibacterial responses have been studied in atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroa(-)) strain of aeromonas salmonicida known to elicit protective immunity. three tissues were studied for transcriptional changes, the liver, head kidney and the gill. rna was collected from fish 6, 12, 24 and 48 h following infection or at the same time points from fish injected with pbs as non-infected control. pcr-select cdna subtraction libraries were con ... | 2006 | 16313960 |
| identification of unusual bacterial glycosylation by tandem mass spectrometry analyses of intact proteins. | the characterization of protein glycosylation can be a complex and time-consuming procedure, especially for prokaryote o-linked glycoproteins, which often comprise unusual oligosaccharide structures with no known glycosylation motif. in this report, we describe a "top-down" approach that provides information on the extent of glycosylation, the molecular masses, and the structure of oligosaccharide residues on bacterial flagella, important structural proteins involved in the motility of pathogeni ... | 2005 | 16316188 |
| adjuvant effect of propolis on immunisation by inactivated aeromonas hydrophila in carp (carassius auratus gibelio). | | 2006 | 16325428 |
| food selection by bacterivorous protists: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization. | a modified fluorescence in situ hybridization (fish) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-proteobacteria -- aeromonas hydrophila and pseudomonas fluorescens, respectively) to grazing by three protists: the flagellates bodo saltans and goniomonas sp., and the ciliate cyclidium glaucoma. both flagellate species prefer ... | 2005 | 16329920 |
| determination of the viability of aeromonas hydrophila in different types of water by flow cytometry, and comparison with classical methods. | the presence of aeromonas spp. in water can represent a risk for human health. therefore, it is important to know the physiological status of these bacteria and their survival in the environment. we studied the behavior of a strain of aeromonas hydrophila in river water, spring water, brackish water, mineral water, and chlorinated drinking water, which had different physical and chemical characteristics. the bacterial content was evaluated by spectrophotometric and plate count techniques. flow c ... | 2005 | 16332772 |
| cell adsorption and selective desorption for separation of microbial cells by using chitosan-immobilized silica. | cell adsorption and selective desorption for separation of microbial cells were conducted by using chitosan-immobilized silica (cis). when chitosan was immobilized onto silica surfaces with glutaraldehyde, bacterial cells adsorbed well and retained viability. testing of the adsorption and desorption ability of cis using various microbes such as escherichia coli, aeromonas hydrophila, pseudomonas aeruginosa, bacillus subtilis, micrococcus luteus, staphylococcus aureus, staphylococcus epidermidis, ... | 2005 | 16332887 |
| production of acylated homoserine lactones by aeromonas and pseudomonas strains isolated from municipal activated sludge. | up to now, the production and role of n-acyl homoserine lactones (ahls) in activated sludge have been poorly understood. in this study, cross-feeding assays with the reporter strains agrobacterium tumefaciens ntl4 and chromobacterium violaceum cv026 were used to investigate ahl signal production by municipal activated sludge samples. ahl signal production was consistently detected from municipal activated sludge when different samples were incubated on nutrient media. from one municipal activate ... | 2005 | 16333331 |
| development of a rapid identification method for aeromonas species by multiplex-pcr. | existing biochemical methods cannot distinguish among some species of aeromonads, while genetic methods are labor intensive. in this study, primers were developed to three genes of aeromonas: lipase, elastase, and dna gyraseb. in addition, six previously described primer sets, five corresponding to species-specific signature regions of the 16s rrna gene from a. veronii, a. popoffii, a. caviae, a. jandaei, and a. schubertii, respectively, and one corresponding to a. hydrophila specific lipase (hy ... | 2005 | 16333335 |
| use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, oncorhynchus mykiss (walbaum). | from a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, oncorhynchus mykiss, and carp, cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by lactococcus garvieae and streptococcus iniae when used as a feed additive. the culture, aeromonas sobria gc2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. whereas the untre ... | 2005 | 16336470 |
| engulfed pathogen-induced apoptosis in haemocytes of giant freshwater prawn, macrobrachium rosenbergii. | haemocytes of the giant freshwater prawn, macrobrachium rosenbergii, were investigated for the induction of apoptosis after phagocytosis of pathogenic yeasts, bacteria and non-pathogenic latex beads in vitro. isolated haemocytes of m. rosenbergii were cultured at a ratio of 1:50 haemocytes to pathogen with the yeast debaryomyces hansenii, the bacteria aeromonas hydrophila or enterococcus faecium, or with latex beads at 25 degrees c for 2 h, followed by washing to remove free particles. at least ... | 2005 | 16336474 |
| macrolide inactivation gene cluster mpha-mrx-mphr adjacent to a class 1 integron in aeromonas hydrophila isolated from a diarrhoeic pig in oklahoma. | to characterize a multidrug-resistant aeromonas hydrophila isolate (cvm861) that possesses a high-level macrolide inactivation gene cluster (mpha-mrx-mphr), previously only reported in escherichia coli. | 2006 | 16339607 |
| impact of non-legionella bacteria on the uptake and intracellular replication of legionella pneumophila in acanthamoeba castellanii and naegleria lovaniensis. | in aquatic environments, legionella pneumophila survives, in association with other bacteria, within biofilms by multiplying in free-living amoebae. the precise mechanisms underlying several aspects of the uptake and intracellular replication of l. pneumophila in amoebae, especially in the presence of other bacteria, remain unknown. in the present study, we examined the competitive effect of selected non-legionella bacteria (escherichia coli, aeromonas hydrophila, flavobacterium breve, and pseud ... | 2005 | 16341636 |
| [identification and phylogenetic study of pathogenic bacteria causing ulcer disease of cultured turbot (scophthalmus maximus)]. | an outbreak of ulcer disease of the cultured turbot scophthalmus maximus l. occurred in an indoor farm in haiyang, shandong province in the sweltering august of 2004 and caused significant mortality. at the beginning, infected turbots displayed sluggish swimming and anorexia. several days later, the turbot eyes swelled, the fins and tail turned red, and the back gradually ulcerated. dissection of the moribund fish showed that the ulcerative gills paled, the liver becamed bloodshot, the kidney an ... | 2005 | 16342752 |
| aeromonas distribution and survival in a thermally altered lake. | par pond is a thermally enriched monomictic southeastern lake which receives heated effluent from a production nuclear reactor. fish populations in the lake have lesions of epizooty from which aeromonas spp. are readily isolated. distribution and population densities of aeromonas in the water column were measured along an oxygen and temperature gradient as well as seasonally. greater population densities of aeromonas occurred below the oxygen chemocline when the lake was stratified. survival of ... | 1977 | 16345182 |
| partial purification and some properties of tyrosine phenol-lyase from aeromonas phenologenes atcc 29063. | tyrosine phenol-lyase was purified 32-fold from aeromonas phenologenes atcc 29063, the organism that produces phenol in refrigerated haddock. the purification procedure included ammonium sulfate fractionation, protamine sulfate treatment, and column chromatography with sephadex g-200, diethyl-aminoethyl-cellulose, and hydroxyapatite. the enzyme was found to be thermally inactivated at temperatures above 40 degrees c. the optimum ph of the enzyme was found to be ph 8.5. the michaelis constants fo ... | 1977 | 16345185 |
| effects of naturally occurring aquatic organic fractions on am uptake by scenedesmus obliquus (chlorophyceae) and aeromonas hydrophila (pseudomonadaceae). | naturally occurring organics were extracted from water collected from skinface pond near aiken, s.c. organics were separated into four nominal diameter size fractions (i, >0.0183; ii, 0.0183 to 0.0032; iii, 0.0032 to 0.0009; iv, <0.0009 mum) by membrane ultrafiltration and introduced into scenedesmus obliquus and aeromonas hydrophila cultures to determine their effects on am availability for uptake. effects on am uptake were determined in actively growing s. obliquus cultures after 96 h of growt ... | 1977 | 16345193 |
| growth of aeromonas hydrophila at low concentrations of substrates added to tap water. | the ability of an aeromonas hydrophila isolate obtained from filtered river water to grow at low substrate concentrations was studied in batch experiments with tap water supplied with low concentrations of substrates. growth was assessed by colony count determinations. the isolate only multiplied in the used tap water (2 to 3 mg of dissolved organic carbon per liter) after the addition of a small amount of an assimilable carbon compound. d-glucose especially caused growth of the organism even at ... | 1980 | 16345582 |
| effects of pharmaceutical wastes on microbial populations in surface waters at the puerto rico dump site in the atlantic ocean. | a series of cruises during 1979 and 1980 to the pharmaceutical dump site located 64 km north of arecibo, puerto rico, in the atlantic ocean, was carried out to evaluate effects of wastes on the ecology of the microflora of surface waters of the dump site. in addition to bacteriological monitoring of the waste plume created by the release of wastes from the disposal barge, stations along a series of transects, extending north from coastal waters through and beyond the dump site, were sampled. lar ... | 1981 | 16345752 |
| nonselectivity of rimler-shotts medium for aeromonas hydrophila in estuarine environments. | thirty presumptive aeromonas hydrophila isolates were collected from estuarine sources using rimler-shotts media. many of the isolates, especially the strains from a low-salinity site, were also identified as a. hydrophila using the api 20e system and two differential techniques devised for the identification of a. hydrophila. all of the isolates, however, had deoxyribonucleic acid base compositions with guanine-plus-cytosine ratios between 40 and 49 mol%, which excludes these strains from the g ... | 1981 | 16345850 |
| external microflora of a marine wood-boring isopod. | bacteria associated with the marine wood-boring isopod limnoria lignorum were enumerated by acridine orange epifluorescence microscopy and by plate counts on several media; the plate-viable bacteria were isolated and identified. similar procedures were followed to enumerate and identify bacteria associated with the wood substrate from which the isopods were collected and with the surrounding water from the isopod habitat. approximately 1.4 x 10 bacterial cells were associated with each individua ... | 1981 | 16345871 |
| diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters. | of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. the isolates were characterized as aeromonas sp., chromobacterium cf. lividum, vibrio sp., and two pseudomonas spp. specific antibodies were produced by immunization of rabbits, and bacterial cells were stained on black uni-pore membrane filters by an indirect immunofluorescent staining procedure. the method proved to be very specific and prac ... | 1982 | 16345920 |
| isolation and characterization of ultramicrobacteria from a gulf coast estuary. | viable bacteria were recovered from estuarine waters passed through a 0.2-mum polycarbonate membrane filter. the recovery method included the use of a dilute nutrient broth for primary enrichment followed by conditioning of the organism to a dilute nutrient solid medium. these bacteria were gram-negative rods and coccobacilli having an nacl requirement and, upon initial culturing, low nutritional requirements. in response to increased nutrient preparations, these microorganisms underwent an incr ... | 1982 | 16345964 |
| isolation and partial characterization of two aeromonas hydrophila bacteriophages. | two aeromonas hydrophila bacteriophages, aeh1 and aeh2, were isolated from sewage. both phages showed binal symmetry. the dimensions of a. hydrophila phages aeh1 and aeh2 differed from those of the other aeromonas phages. also, phage aeh2 was the largest aeromonas phage studied to date. phage aeh1 formed small, clear plaques, and phage aeh2 formed turbid plaques with clear centers. both phages were sensitive to chloroform treatment, being totally inactivated after treatment for 1 h at 60 degrees ... | 1983 | 16346300 |
| predominant bacteria in an activated sludge reactor for the degradation of cutting fluids. | for the first time, an activated sludge reactor, established for the degradation of cutting fluids, was examined for predominant bacteria. in addition, both total and viable numbers of bacteria in the reactor were determined so that the percentage of each predominant type in the total reactor population could be determined. three samples were studied, and a total of 15 genera were detected. in each sample, the genus pseudomonas and the genus microcyclus were present in high numbers. three other ... | 1983 | 16346426 |
| starch-ampicillin agar for the quantitative detection of aeromonas hydrophila. | interest in aeromonas hydrophila as a food-borne and human pathogen is increasing. isolation media from the clinical laboratory were evaluated for food use and either did not give quantitative recovery of a. hydrophila or did not permit ready differentiation of a. hydrophila from the background microflora. a new medium was developed which permitted quantitative recovery of a. hydrophila from foods. the medium consisted of phenol red agar base (difco laboratories), soluble starch (10 g/liter), an ... | 1985 | 16346899 |
| significance of extracellular protease for growth of a heterotrophic bacterium, aeromonas salmonicida. | the role of protease produced by a heterotrophic bacterium during growth was investigated with aeromonas salmonicida, the pathogen of fish furunculosis, strain a-7301 and its protease-deficient mutant ntg-1 induced by mutagenesis. strain a-7301 produced extracellular protease in a mixed amino acid medium (composed of gly, ala, val, ile, leu, thr, ser, cys, met, phe, tyr, lys, arg, pro, his, try, asp, asn, glu, and gln at equal concentrations of 0.1 g/liter). its multiplication rate was limited b ... | 1985 | 16346900 |
| temporal occurrence of vibrio species and aeromonas hydrophila in estuarine sediments. | marine sediments were assayed for their concentration of vibrio spp. and aeromonas hydrophila over 1 year. a temporal variation was observed in which a. hydrophila, and to a lesser degree v. fluvialis, were found in the winter months, v. parahaemolyticus and v. vulnificus predominated in the spring and summer, with non-o-1 v. cholerae and v. alginolyticus detected in the late summer and fall. these organisms were found in greatest numbers in the top 5 cm of sediment, but were detectable down to ... | 1985 | 16346948 |
| evaluation of different assay systems for identification of environmental aeromonas strains. | important biochemical reactions in conventional tests were compared with counterpart reactions in two multiple test systems, api-20e (analytab products, plainview, n.y.) and aeromonas hydrophila medium, to evaluate their accuracy for the identification of motile aeromonas spp. isolated from fish. in a total of 49 aeromonas spp. isolates and 10 a. hydrophila reference strains, false-negative or -positive reactions were detected in the voges-proskauer test, indole production, gelatinase activity, ... | 1986 | 16347025 |
| evaluation of different assay systems for identification of environmental aeromonas strains. | [this corrects the article on p. 655 in vol. 51.]. | 1986 | 16347094 |
| electrostatic mechanism of survival of virulent aeromonas salmonicida strains in river water. | [this corrects the article on p. 1343 in vol. 51.]. | 1986 | 16347195 |
| bacterial colonization of cod (gadus morhua l.) and halibut (hippoglossus hippoglossus) eggs in marine aquaculture. | aquaculture has brought about increased interest in mass production of marine fish larvae. problems such as poor egg quality and mass mortality of fish larvae have been prevalent. the intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. little information about the adherent microflor ... | 1989 | 16347937 |
| purification and properties of beta-1, 4-xylanase from aeromonas caviae w-61. | aeromonas caviae w-61, which was isolated from water samples at the faculty of agriculture, tohoku university, produced beta-1, 4-xylanase (1,4-beta-d-xylan xylanohydrolase; ec 3.2.1.8) extracellularly. the xylanase was purified to homogeneity by using deae-sephadex a-50, cm-sephadex c-50, and sephadex g-100 column chromatographies. the molecular weight of the purified enzyme was estimated to be 22,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the isoelectric point of the enz ... | 1991 | 16348410 |
| mineralization of linear alkylbenzene sulfonate by a four-member aerobic bacterial consortium. | a bacterial consortium capable of linear alkylbenzene sulfonate (las) mineralization under aerobic conditions was isolated from a chemostat inoculated with activated sludge. the consortium, designated kjb, consisted of four members, all of which were gram-negative, rod-shaped bacteria that grew in pairs and short chains. three isolates had biochemical properties characteristic of pseudomonas spp.; the fourth showed characteristics of the aeromonas spp. cell suspensions were grown together in min ... | 1991 | 16348496 |
| evidence for bacterial chemotaxis to cyanobacteria from a radioassay technique. | lyngbya birgei and aphanizomenon flos-aquae elicited a significant chemotactic attraction of aeromonas hydrophila compared with controls lacking cyanobacteria. there was a positive exponential relationship between biomass (chlorophyll a) of l. birgei and a. flos-aquae and chemotactic attraction of a. hydrophila. the assay equipment was simple and reliable and could be used to study bacterial chemotaxis in other species in situ. | 1991 | 16348544 |
| molecular cloning of the tryptophan operon from an aeromonas hydrophila freshwater isolate. | a genomic library of aeromonas hydrophila f9 was constructed by using pbr322 as a vector. from that, two dna fragments (5.8 and 11.6 kb) were isolated containing genetic information to complement trpa and trpb defects (5.8-kb fragment) and to complement trpa, trpb, trpc, trpd, and trpe defects (11.6-kb fragment) in escherichia coli mutants. evidence of the existence of a secondary promoter is given. | 1992 | 16348662 |