| inhibition of neomycin phosphorotransferase expression in entamoeba histolytica with antisense peptide nucleic acid (pna) oligomers. | | 2000 | 11070312 |
| entamoeba histolytica hybrids. | | 2000 | 11070313 |
| cis-elements upregulate the activity of the entamoeba histolytica ehpgp1 gene promoter. | | 2000 | 11070314 |
| the multidrug-resistance phenotype of entamoeba histolytica-transfected trophozoites with the ehpgp5 gene. | | 2000 | 11070315 |
| algorithm to predict minichef electrophoresis patterns of entamoeba histolytica dna. | | 2000 | 11070316 |
| an initial characterization of the 3' untranslated region of the ehpgp5 mrna in entamoeba histolytica. | | 2000 | 11070317 |
| expression of entamoeba histolytica ehpgp5 gene in xenopus laevis oocytes. | | 2000 | 11070318 |
| expression and immunodetection of a p-glycoprotein in emetine-resistant trophozoites of entamoeba histolytica. | | 2000 | 11070319 |
| possible role of the ccaat/enhancer binding protein in the expression regulation of the ehpgp1 multidrug resistance gene in entamoeba histolytica. | | 2000 | 11070320 |
| dna cloning of the entamoeba histolytica prp6 gene: a putative u4/u6 small nuclear ribonucleoprotein particle (snrnp). | | 2000 | 11070321 |
| presence of sequences homologous to the universal minicircle sequence (ums) of trypanosomatids in the dna circles of entamoeba histolytica. | | 2000 | 11070322 |
| dna binding activity and predicted tertiary structure of the tata binding protein of entamoeba histolytica. | | 2000 | 11070323 |
| the tail domain of entamoeba histolytica gef: a guanine nucleotide exchange factor. | | 2000 | 11070324 |
| structural characterization of the 5'-flanking regions of the entamoeba histolytica ehcp112 and ehadh112 genes. | | 2000 | 11070325 |
| isolation and identification of l10 gene from entamoeba histolytica homologous to wilms' tumor suppressor. | | 2000 | 11070326 |
| immunogenicity of an optimized entamoeba histolytica gal-lectin dna vaccine. | | 2000 | 11070327 |
| isolation of phage mimotopes mimicking a protective epitope of gpi-linked proteophosphoglycan antigens of entamoeba histolytica. | | 2000 | 11070328 |
| bacterial expression of a human monoclonal antibody that inhibits in vitro adherence of entamoeba histolytica trophozoites. | | 2000 | 11070329 |
| cloning and functional expression of rpn1, a regulatory-particle non-atpase subunit 1, of proteasome from trypanosoma cruzi. | non-lysosomal protein degradation in eukaryotic cells involves a proteolytic complex referred to as 26s proteasome that consists of a 20s core particle and one or two 19s regulatory particles. we have cloned the gene rpn1 encoding rpnl (regulatory-particle non-atpase subunit 1), one of the largest subunits of proteasome, from trypanosoma cruzi. it contains 2712 bp and encodes 904 amino acid residues with a calculated molecular mass of 98.2 kda and an isoelectric point of 5.2. the predicted amino ... | 2000 | 11071286 |
| a simple and reliable 5'-race approach. | a novel approach for the amplification of cdna ends is described. it requires only minimal amounts of material, a simple cdna synthesis reaction and a single pcr reaction to amplify the desired 5'- or 3'-ends of a certain cdna of interest. it combines the so called capfinder approach with solid phase cdna synthesis, thus almost eliminating background problems usually associated with 5'-race protocols. this approach could be used to generate complete 5'-ends of numerous cdnas using only one cdna ... | 2000 | 11071950 |
| [diagnostic image (8). amebic colitis]. | a 34-year-old woman suffered from progressive abdominal pain and diarrhoea. laparotomy revealed colonic perforation due to amebic colitis. | 2000 | 11072508 |
| the evolution of entamoeba, a cautionary tale. | in addition to its medical importance, the parasite entamoeba histolytica has been studied by evolutionary biologists because of its unusual structural and biochemical features. recent molecular data have revealed that many of our assumptions regarding the relationships of the organism and its relatives have been incorrect. | 2000 | 11081575 |
| acute appendicitis caused by amebiasis. | we report a case of appendicitis caused by amebiasis in a 45-year-old japanese man. he presented to our hospital with bloody stools in june 1998. sigmoidoscopy disclosed erosion, and a biopsy of the erosion showed colitis caused by entamoeba histolytica infection. four months later, he was admitted to our hospital with a small elastic mass and severe pain in the lower quadrant of the abdomen, which was diagnosed as acute appendicitis. he underwent appendectomy. histopathological examination reve ... | 2000 | 11085496 |
| amebiasis: clinical implications of the recognition of entamoeba dispar. | entamoeba histolytica was recently reclassified to recognize the existence of two genetically distinct but morphologically indistinguishable species: e. histolytica, the protozoan parasite that causes amebic dysentery and liver abscess, and entamoeba dispar, a nonpathogenic intestinal parasite. acceptance of this redefinition has dramatically changed both our understanding of the true epidemiology of e. histolytica and the optimal methods for diagnosing amebiasis. molecular-based diagnostic test ... | 1999 | 11095821 |
| the endocytic pathway in entamoeba histolytica. | we studied the endocytic pathway of entamoeba histolytica using (a) confocal laser scanning microscopy to observe living cells labeled with fluorescent probes and (b) transmission electron microscopy of cells incubated in the presence of gold-labeled proteins or in a cytochemical medium designed for the localization of acid phosphatase activity. images of acridine-orange-labeled cells showed that most of the intracellular vacuoles were acidic and were also labeled with lucifer yellow, a fluoresc ... | 2000 | 11097295 |
| comparative study of the efficacy and tolerability of secnidazole suspension (single dose) and tinidazole suspension (two days dosage) in the treatment of amebiasis in children. | amebiasis caused by entamoeba histolytica may be considered the most aggressive parasitic disease affecting human intestine, causing acute amoebic colitis and extra-intestinal diseases of high morbidity and mortality. 5-nitroimidazoles are the drugs of choice. in this multicenter, open and random clinical trial, the efficacy and tolerability of secnidazole suspension in a single oral dose of 1ml/kg was compared with 0.5ml/kg doses of tinidazole suspension given for 2 consecutive days to 303 enta ... | 1999 | 11098194 |
| the application of the atomic force microscope to studies of medically important protozoan parasites. | both living and fixed specimens of the medically-important parasitic protozoa, trypanosoma cruzi, toxoplasma gondii, giardia lamblia, entamoeba histolytica, and acanthamoeba spp. were studied by atomic force microscopy (afm). the preparation of fixed specimens was similar to methods used for either scanning or transmission electron microscopy. afm scanning was performed using both contact and tapping modes. a classical fixation procedure utilizing glutaraldehyde followed by ethanol dehydration w ... | 2000 | 11108031 |
| two novel calcium-binding proteins from cytoplasmic granules of the protozoan parasite entamoeba histolytica. | we report on the molecular characterisation of two novel granule proteins of the protozoon and human pathogen entamoeba histolytica. the proteins, which were named grainin 1 and 2, show a considerable structural similarity to calcium-binding proteins, particularly within ef-hand motifs. each grainin possesses three of these putative calcium-binding sites. based on careful inspection of known structures of protein families containing ef-hands, a domain of grainin 1 covering two ef-hand motifs was ... | 2000 | 11113449 |
| [bacteriological quality of human drinking water in high-margination zones in chiapas]. | to analyze the bacteriological quality of drinking water (bqdw) and its association with diarrhoea and intestinal parasites in children 1 to 14 years old, in areas of high levels of poverty in chiapas, mexico. | 2000 | 11125624 |
| socioeconomic status, clinical features, laboratory and parasitological findings of hepatic amebiasis patients--a hospital based prospective study in bangladesh. | socioeconomic status, clinical, laboratory and parasitological features of 31 hospitalized amebic liver abscess (ala) and 8 amebic hepatitis (ah) patients were studied. thirty-seven (94.9%) of the total 39 cases were from low socioeconomic class and 2 (5.1%) were from middle class (p<0.001). sixteen (51.6%) ala and 5 (62.5%) ah patients were admitted with duration of disease for 3 weeks or more. twenty-one (67.7%) ala and 3 (37.5%) ah cases gave no previous history of diarrhea or dysentery. epig ... | 2000 | 11127347 |
| auxotrophy to lipoproteins of entamoeba histolytica cultivated under axenic conditions. | entamoeba histolytica grows in media without serum but with a mixture of aminoacids, vitamins, lipoproteins, free cholesterol, phospholipids and fatty acids called pacsr. the ability of lipoproteins and free lipids to support growth of three e. histolytica strains (hk9, hmi:imss and hm3:imss) was analysed. tubes containing 5 ml culture medium, amino acids, vitamins and either 120-1,200 microg lipoproteins/ml or 0.017-0.10 mg free lipids/ml (predissolved in absolute ethanol) were inoculated with ... | 2000 | 11133106 |
| evaluation of the triage micro parasite panel for detection of giardia lamblia, entamoeba histolytica/entamoeba dispar, and cryptosporidium parvum in patient stool specimens. | a study comparing the triage micro parasite panel (biosite diagnostics, inc., san diego, calif.) to conventional o&p examination (o&p) was performed using patient fecal specimens. five hundred twenty-three stool samples were compared. nineteen specimens were found to be positive by triage, and 29 were found to be positive by o&p. seven specimens were positive for giardia lamblia, four were positive for entamoeba histolytica/e. dispar, and three were positive for cryptosporidium parvum as determi ... | 2001 | 11136793 |
| recent developments in amoebiasis:the gal/galnac lectins of entamoeba histolytica and entamoeba dispar. | amoebiasis is responsible for 50000-100000 deaths annually. invasive amoebic disease begins with the attachment of entamoeba histolytica trophozoites to colonic mucin, a process mediated by the amoebic gal/galnac lectin. the non-pathogenic counterpart, e. dispar, is morphologically identical but genetically distinct. investigations comparing the gal/galnac lectin from these two organisms are under way. | 2000 | 11137050 |
| [a case of aids complicated with liver tuberculosis]. | we experienced a double infection of tuberculosis and amebiasis of the liver. a 28 year old male with aids was admitted to our hospital because of severe diarrhea and liver abscess by entamoeba histolytica. in spite of improvement of the diarrhea and liver abscess by the therapy against e. historicica, serum levels of gamma-gtp and alp remained high and hepatosplenomegaly gradually increased. a liver biopsy was performed. pathology showed a granulomatous lesion with langhans' giant cells. from t ... | 2000 | 11140084 |
| experimental amebiasis: immunohistochemical study of immune cell populations. | distribution of immune cell populations was studied in a c3h/hej mouse model of intestinal amebiasis from 5 to 60 days post-inoculation with entamoeba histolytica, using immunoperoxidase techniques. at various time intervals, the ceca from mice were fixed in 10% formalin, dehydrated, embedded and sectioned at 5 microm. sections were incubated with conjugated peroxidase-labelled antibodies to mouse iga, igm, and igg. color was developed with 3, 3'-diaminobenzidine tetrahydrochloride (dab)/h2o2 so ... | 2000 | 11140454 |
| in vitro antiplasmodial, antiamoebic, and cytotoxic activities of some monomeric isoquinoline alkaloids. | twenty-one alkaloids have been assessed for activities against plasmodium falciparum (multidrug- resistant strain k1) in vitro; 18 of these are reported for the first time. two protoberberine alkaloids, dehydrodiscretine and berberine, were found to have antiplasmodial ic(50) values less than 1 m, while seven alkaloids-allocrytopine, columbamine, dehydroocoteine, jatrorrhizine, norcorydine, thalifendine, and ushinsunine-had values between 1 and 10 m. these results are discussed in the context of ... | 2000 | 11141105 |
| drug targets and mechanisms of resistance in the anaerobic protozoa. | the anaerobic protozoa giardia duodenalis, trichomonas vaginalis, and entamoeba histolytica infect up to a billion people each year. g. duodenalis and e. histolytica are primarily pathogens of the intestinal tract, although e. histolytica can form abscesses and invade other organs, where it can be fatal if left untreated. t. vaginalis infection is a sexually transmitted infection causing vaginitis and acute inflammatory disease of the genital mucosa. t. vaginalis has also been reported in the ur ... | 2001 | 11148007 |
| development of monoclonal antibodies which specifically recognize entamoeba histolytica in preserved stool samples. | we report the generation of monoclonal antibodies against a recombinant 170-kda subunit of the gal or galnac lectin of entamoeba histolytica that specifically recognize e. histolytica but not entamoeba dispar in preserved stool samples. these antibodies do not cross-react with other bowel protozoa, including entamoeba coli, giardia lamblia, and dientamoeba fragilis. | 2001 | 11158133 |
| partial purification and characterization of dolichol phosphate mannose synthase from entamoeba histolytica. | dolichol phosphate mannose synthase, an essential enzyme in glycoprotein biosynthesis, was partially purified from e.histolytica by hydrophobic interaction and affinity chromatography with octyl sepharose cl-4b and affi-gel 501, respectively. reducing agents, particularly dithiothreitol, positively influenced enzyme activity and stability, indicating a role of sulfhydryl groups on the transferase function. activity did not depend on phospholipids; however, it was significantly stimulated by phos ... | 2000 | 11159922 |
| trichomonas vaginalis: chromatin and mitotic spindle during mitosis. | the mitotic phases and the changes that the chromatin and mitotic microtubules undergo during mitosis in the sexually transmitted parasite trichomonas vaginalis are described. parasites arrested in the gap 2 phase of the cell cycle by nutrient starvation were induced to mitosis by addition of fresh whole medium. [(3)h] thymidine labeling of trichomonad parasites for 24 h showed that parasites have at least four synchronic duplications after mitosis induction. fixed or live and acridine orange (a ... | 2000 | 11162363 |
| molecular cloning and cellular localization of an unusual bipartite entamoeba histolytica polypeptide with similarity to actin binding proteins. | | 2000 | 11163453 |
| the unusual transhydrogenase of entamoeba histolytica. | we have expressed and purified a protein fragment from entamoeba histolytica. it catalyses transhydrogenation between analogues of nad(h) and nadp(h). the characteristics of this reaction resemble those of the reaction catalysed by a complex of the nad(h)- and nadp(h)-binding subunits of proton-translocating transhydrogenases from bacteria and mammals. it is concluded that the complete en. histolytica protein, which, along with similar proteins from other protozoan parasites, has an unusual subu ... | 2001 | 11163794 |
| granulysin: a novel antimicrobial. | granulysin is a novel lytic molecule produced by human cytolytic t-lymphocytes (ctls) and natural killer (nk) cells. it is active against a broad range of microbes, including gram-positive and -negative bacteria, parasites and mycobacterium tuberculosis. it is functionally related to other antibacterial peptides, like defensins and magainins, but is structurally distinct. it has structural similarity to porcine nk-lysin and to amoebapores made by entamoeba histolytica. synthetic peptides derived ... | 2001 | 11178344 |
| a review of plant species assessed in vitro for antiamoebic activity or both antiamoebic and antiplasmodial properties. | the resurgence of the protozoal diseases amoebiasis and malaria has been known to occur, from time to time, in endemic and epidemic proportions all over the world. furthermore, the import of these individual pathogens to other areas from tropical regions encourages these protozoal diseases to occur on a global scale with considerable associated mortality and morbidity. from time immemorial, the cure of these diseases has been attempted with the use of traditional plant products, derived from suc ... | 2001 | 11180515 |
| ruptured liver abscess with fulminant amoebic colitis: case report with review. | amoebic liver abscess is the commonest extra intestinal manifestation of amoebiasis. intraperitoneal rupture of liver abscess and fulminant necrotizing amoebic colitis are rare occurrences which complicate a severe form of invasive disease caused by entamoeba histolytica. these complications are associated with a high morbidity and mortality. synchronous pathological lesions in colon and liver are rare. still rare is the occurrence of complicated colonic and hepatic invasive amoebiasis presentin ... | 2000 | 11194588 |
| high prevalence of infection with entamoeba dispar, but not e. histolytica, in captive macaques. | a total of 268 nonhuman primates (20 species) kept in the primate research institute, kyoto university, japan, were surveyed for intestinal amebas. total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: entamoeba histolytica/e. dispar, 53%; e. coli, 34%; e. hartmanni, 34%; iodamoeba buetschlii, 25%; endolimax nana, 8%; and e. chattoni, 3%. positive rates were higher in old world monkeys and lower in new world monkeys. all the 1 ... | 2001 | 11199843 |
| entamoeba histolytica: production of nitric oxide and in situ activity of nadph diaphorase in amebic liver abscess of hamsters. | entamoeba histolytica trophozoites were inoculated into the liver of hamsters and serum nitrate/nitrite levels [expressed as nitric oxide (no) production] were determined at different times during amebic liver abscess (ala) development. we also tested the effects of no synthase (nos) inhibitors such as n(g)-nitro-l-arginine methyl ester (l-name), aminoguanidine, and dexamethasone during ala production. since nos activity has been correlated with expression of reduced nicotinamide adenine dinucle ... | 2001 | 11199849 |
| protection of hamsters from amebic liver abscess formation by immunization with the 150- and 170-kda surface antigens of entamoeba histolytica. | a monoclonal antibody, eh3015, prevents in vitro adherence of entamoeba histolytica trophozoites to mammalian cells and inhibits amebic liver abscess formation in hamsters. by immunoaffinity chromatography with the monoclonal antibody, purified e. histolytica antigens with molecular masses of 150 and 170 kda under non-reduced conditions were obtained. hamsters were immunized with these antigens (group i) or with fractions further purified by polyacrylamide gel electrophoresis (group ii). pooled ... | 2001 | 11206109 |
| synthesis and antiparasitic activity of 2-(trifluoromethyl)-benzimidazole derivatives. | 2-(trifluoromethyl)benzimidazole derivatives substituted at the 1-, 5-, and 6-positions have been synthesized and in vitro tested against the protozoa giardia lamblia, entamnoeha histolytica. and the helminth trichinella spiralis. results indicate that all the compounds tested are more active as antiprotozoal agents than albendazole and metronidazole. one compound (20) was as active as albendazole against t. spiralis. these compounds were also tested for their effect on tubulin polymerization an ... | 2001 | 11206455 |
| new insights into the role of the cytoskeleton in phagocytosis of entamoeba histolytica. | entamoeba histolytica, a human parasite, crosses the natural barriers of the intestine and, in turn, spreads into the deeper organs, resulting in amoebiasis. the motility of the parasite and its ability to lyse or phagocytose human cells facilitates passage of the amoeba through the intestinal epithelium. little is known about the uptake of material by this parasite; nevertheless, the cytoskeleton is believed to play a role in phagocytosis. myosin ib, an actin-binding protein, localizes to the p ... | 1999 | 11207552 |
| actin induction during pma and camp-dependent signal pathway activation in entamoeba histolytica trophozoites. | activation of pkc or camp-dependent signalling pathways in entamoeba histolytica triggers the phosphorylation of proteins involved in actin rearrangements necessary for adhesion and locomotion. analogous motifs to sre and cre sequences--known to respond to pma and camp--were identified within the 5' regulatory region (5'rr) of one of the parasite actin genes. these sequences could be involved in the actin transcriptional upregulation reported during signalling. to test this hypothesis, a plasmid ... | 2000 | 11207595 |
| caspase 3-dependent killing of host cells by the parasite entamoeba histolytica. | the parasite entamoeba histolytica is named for its ability to lyse host tissues. to determine the factors responsible, we have initiated an examination of the contribution of parasite virulence factors and host caspases to cellular destruction by the parasite. amoebic colitis in c3h/hej mice was associated with extensive host apoptosis at sites of e. histolytica invasion. in vitro studies of e. histolytica-jurkat t-cell interactions demonstrated that apoptosis required contact via the amoebic g ... | 2000 | 11207613 |
| overexpression of cysteine proteinase 2 in entamoeba histolytica or entamoeba dispar increases amoeba-induced monolayer destruction in vitro but does not augment amoebic liver abscess formation in gerbils. | to study the role of cysteine proteinases in the pathogenicity of entamoeba histolytica, we have attempted to overexpress the three main cysteine proteinases (ehcp1, ehcp2, ehcp5) of this parasite in trophozoites of e. histolytica as well as in non-pathogenic entamoeba dispar by episomal transfection. although each of the corresponding coding sequences were cloned in identical expression plasmids, we were unable to overexpress ehcp1 and ehcp5, respectively, but could substantially induce express ... | 2001 | 11207616 |
| secondary infections of aids autopsy cases in japan with special emphasis on mycobacterium avium-intracellulare complex infection. | in order to study the frequency of secondary infections of aids autopsy cases in japan, especially the frequency of mycobacterium aviumintracellulare complex (mac) infection, retrospective autopsy study was conducted between 1986 and 1997 at the affiliated hospital of institute of medical sciences, university of tokyo. secondary infections of various organs from 43 aids autopsy cases were examined using histopathology, genetic diagnosis of tuberculosis, ziehl-neelsen stain for acid-fast bacilli ... | 2000 | 11211316 |
| a novel anti-inflammatory oligopeptide produced by entamoeba histolytica. | the monocyte locomotion inhibitory factor (mlif), a heat-stable oligopeptide found in the supernatant fluid of entamoeba histolytica axenic cultures was isolated by ultra-filtration, gel-sieve chromatography and high powered liquid chromatography (hplc), and its primary structure (met-gln-cys-asn-ser) established by edman sequencing and mass-spectrometry (ms). a synthetic peptide had the same selective anti-inflammatory features as the native material in comparable concentrations: in vitro inhib ... | 2001 | 11223127 |
| the cell cycle of entamoeba invadens during vegetative growth and differentiation. | the cell division cycle of entamoeba invadens was studied during vegetative growth of trophozoites and during their differentiation into cysts. during vegetative growth of trophozoites, it was observed that dna synthesis typically continued after one genome content had been duplicated. during encystation, dna synthesis was arrested after 4n genome content had been synthesised. using multi-parameter flow cytometry, the light scattering properties of cysts and trophozoites were studied. the cytopl ... | 2001 | 11223134 |
| [diagnostic image (8). intestinal amoebiasis]. | | 2001 | 11225255 |
| a plasma membrane-type ca(2+)-atpase co-localizes with a vacuolar h(+)-pyrophosphatase to acidocalcisomes of toxoplasma gondii. | ca(2+)-atpases are likely to play critical roles in the biochemistry of toxoplasma gondii, since these protozoa are obligate intracellular parasites and the ca(2+) concentration in their intracellular location is three orders of magnitude lower than in the extracellular medium. here, we report the cloning and sequencing of a gene encoding a plasma membrane-type ca(2+)-atpase (pmca) of t.gondii (tga1). the predicted protein (tga1) exhibits 32-36% identity to vacuolar ca(2+)-atpases of trypanosoma ... | 2001 | 11226155 |
| current problems of amebiasis in japan and recent advances in amebiasis research. | amebiasis is epidemic in two major populations in japan: male homosexuals and institutionalized people. currently available diagnostic, chemotherapeutic, and prophylactic measures and their problems are discussed. recent advances in basic research on amebiasis are also described with new findings of unique metabolisms and intracellular structures and organization. discoveries and analyses of the unique features presented by this parasitic protist help in our elucidation of the pathogenic mechani ... | 2000 | 11227020 |
| revisiting amebiasis. | | 2001 | 11228009 |
| role of dna probes in characterization of pathogenic and non-pathogenic e. histolytica. | various tests have been described to differentiate the pathogenic and non-pathogenic types of e. histolytica. recently dna hybridization has been described to differentiate between the two subtypes. using common hmc probe the presence of e. histolytica in stool was confirmed. then on the basis of hybridization with dna probe p 145 (pathogenic) and b 133 (non-pathogenic) e. histolytica was characterized as being pathogenic and non pathogenic respectively. out of 137 patients studied 88 were sympt ... | 1998 | 11229277 |
| isolation and characterization of polymorphic dna from entamoeba histolytica. | an important gap in our understanding of the epidemiology of amebiasis is what determines the outcome of entamoeba histolytica infections. to investigate the possible existence of invasive and noninvasive strains as one factor, the ability to differentiate individual isolates of e. histolytica is necessary. two new loci containing internal repeats, locus 1-2 and locus 5-6, have been isolated. each contains a single repeat block with two types of related direct repeats arranged in tandem. souther ... | 2001 | 11230401 |
| identification of enteropathogenic escherichia coli in simian immunodeficiency virus-infected infant and adult rhesus macaques. | enteropathogenic escherichia coli (epec) was recognized as a common opportunistic pathogen of simian immunodeficiency virus-infected rhesus macaques (macaca mulatta) with aids. retrospective analysis revealed that 27 of 96 (28.1%) animals with aids had features of epec infection, and epec was the most frequent pathogen of the gastrointestinal tract identified morphologically. in 7.3% of animals dying with aids, epec represented the sole opportunistic agent of the gastrointestinal tract at death. ... | 2001 | 11230413 |
| specific detection of entamoeba histolytica dna by hemolysin gene targeted pcr. | diagnostic differentiation of pathogenic entamoeba histolytica from non-pathogenic entamoeba dispar is of great clinical importance. we have developed and evaluated a new polymerase chain reaction (pcr) assay (haemo-pcr) based on the novel e. histolytica hemolysin gene hly6. the specificity of this assay was confirmed by analyzing different entamoeba species, faeces samples, human and bacterial dna, and digestion of amplification products with appropriate restriction enzymes. the sensitivity was ... | 2001 | 11230821 |
| getting sense and finding function in protozoa. | | 2001 | 11231546 |
| inhibition of gene expression in entamoeba histolytica with antisense peptide nucleic acid oligomers. | peptide nucleic acids (pnas) may be a potent tool for gene function studies in medically important parasitic organisms, especially those that have not before been accessible to molecular genetic knockout approaches. one such organism is entamoeba histolytica, the causative agent of amebiasis, which infects about 500 million people and is the cause of clinical disease in over 40 million each year, mainly in the tropical and subtropical world. we used pna antisense oligomers to inhibit expression ... | 2001 | 11231555 |
| gastrointestinal parasites of mountain gorillas (gorilla gorilla beringei) in the parc national des volcans, rwanda. | ninety-eight fecal samples were collected from 74 free-living mountain gorillas (gorilla gorilla beringei) from the parc national des volcans, rwanda, between july 1995 and january 1997 and examined for parasites by sheather's sugar and zinc sulfate flotation methods, trichrome staining, and larval cultures. all samples contained at least one parasite. seventeen endoparasites were identified, including eight protozoa, seven nematodes, one cestode, and one trematode. two species of arthropod mite ... | 2000 | 11237138 |
| amebiasis. | human amebiasis is caused by the protozoan parasite entamoeba histolytica. invasion of the colonic mucosa by the trophozoite form of this parasite generally results in the clinical syndrome of amebic dysentery, although there is a wide range of clinical presentations. some patients will progress to disseminated disease, with resulting amebic liver abscesses. although it is estimated that 10% of the world's population is infected with e. histolytica, only 1% of these patients will manifest diseas ... | 2001 | 11246030 |
| a role for a galactose lectin and its ligands during encystment of entamoeba. | in the life cycle of entamoeba parasites alternate between the colon-dwelling trophozoite and the infectious cyst forms. the physiologic stimuli that trigger differentiation of trophozoites into cysts remain undefined. on the surface of the human-infecting entamoeba, parasites express a galactose/n-acetylgatactosamine (gal/galnac)-binding lectin, which plays demonstrated roles in contact-dependent lysis of target cells and resistance to host complement. using a reptilian parasite, entamoeba inva ... | 2001 | 11249188 |
| the two phosphofructokinase gene products of entamoeba histolytica. | two phosphofructokinase genes have been described previously in entamoeba histolytica. the product of the larger of the two genes codes for a 60-kda protein that has been described previously as a pyrophosphate (pp(i))-dependent enzyme, and the product of the second, coding for a 48-kda protein, has been previously reported to be a pp(i)-dependent enzyme with extremely low specific activity. here it is found that the 48-kda protein is not a pp(i)-dependent enzyme but a highly active atp-requirin ... | 2001 | 11262402 |
| new tools for genetic analysis of entamoeba histolytica: blasticidin s deaminase and green fluorescence protein. | | 2001 | 11267932 |
| cyclospora infection in acquired immunodeficiency syndrome. | diarrhea is a common clinical manifestation of human immunodeficiency virus (hiv) infection. the important protozoan pathogens causing diarrhea include cryptosporidium, microsporidium, isospora belli and cyclospora besides giardia lamblia and entamoeba histolytica. | 2000 | 11273176 |
| the bifunctional entamoeba histolytica alcohol dehydrogenase 2 (ehadh2) protein is necessary for amebic growth and survival and requires an intact c-terminal domain for both alcohol dahydrogenase and acetaldehyde dehydrogenase activity. | the intestinal protozoan pathogen entamoeba histolytica lacks mitochondria and derives energy from the fermentation of glucose to ethanol with pyruvate, acetyl enzyme co-a, and acetaldehyde as intermediates. a key enzyme in this pathway may be the 97-kda bifunctional e. histolytica alcohol dehydrogenase 2 (ehadh2), which possesses both alcohol dehydrogenase (adh) and acetaldehyde dehydrogenase activity (aldh). ehadh2 appears to be a fusion protein, with separate n-terminal aldh and c-terminal ad ... | 2001 | 11274185 |
| identification and characterization of an entamoeba histolytica upstream regulatory element 3 sequence-specific dna-binding protein containing ef-hand motifs. | the hgl5 gene of entamoeba histolytica is negatively regulated through the upstream regulatory element 3 (ure3) dna motif tattctatt. this motif is also present and significant in the function of the e. histolytica fdx gene promoter. a yeast one-hybrid screen was used to identify an e. histolytica cdna encoding a protein (ure3-bp) that recognized this dna motif. analysis of the predicted amino acid sequence demonstrated the presence of two ef-hand motifs but identified no canonical dna binding mo ... | 2001 | 11278344 |
| unusual presentation of amebic liver abscess with thrombocytopenia and splenomegaly. | | 2001 | 11285161 |
| comparison of antigen-capture elisa to stool-culture methods for the detection of asymptomatic entamoeba species infection in kafer daoud, egypt. | we performed a prospective field study in the village of kafer daoud in menofia, egypt to compare the fecal culture method with enzyme linked immuno assay (elisa) for detection of 170 kda lectin antigen in feces for diagnosis of asymptomatic entamoeba histolytica and entamoeba dispar infection. all subjects with e. histolytica or e. dispar infection detected by culture also had positive elisa for amebic antigen in their feces and an additional 57 entameoba infections missed by culture were detec ... | 2000 | 11289667 |
| amebic infection in the human colon induces cyclooxygenase-2. | we sought to determine if infection of the colon with entamoeba histolytica induces the expression of cyclooxygenase-2 and, if it does, to determine the contribution of prostaglandins produced through cyclooxygenase-2 to the host response to amebic infection. human fetal intestinal xenografts were implanted subcutaneously in mice with severe combined immunodeficiency and allowed to grow; the xenografts were then infected with e. histolytica trophozoites. infection with e. histolytica resulted in ... | 2001 | 11292761 |
| amoebicidal and giardicidal compounds from the leaves of zanthoxylum liebmannianun. | the crude ethanol extract from the leaves of zanthoxylum liebmannianum exhibited inhibitory effect on the reproduction of trophozoites of entamoeba histolytica (ic(50)=3.48 microg/ml) and giardia lamblia (ic(50)=58.00 microg/ml). from this extract, asarinin, hyperin, beta-sitosterol, and beta-sitosterol glucoside were isolated. among them, asarinin was the most active with ic(50) values of 19.86 microg/ml for e. histolytica and 35.45 microg/ml for g. lamblia. the remaining compounds showed moder ... | 2001 | 11295310 |
| effects of miltefosine and other alkylphosphocholines on human intestinal parasite entamoeba histolytica. | the protozoan parasite entamoeba histolytica is the cause of amoebic dysentery and liver abscess. it is therefore responsible for significant morbidity and mortality in a number of countries. infections with e. histolytica are treated with nitroimidazoles, primarily with metronidazole. at this time, there is a lack of useful alternative classes of substances for the treatment of invasive amoebiasis. alkylphosphocholines (alkyl-pcs) such as hexadecyl-pc (miltefosine) were originally developed as ... | 2001 | 11302818 |
| effect of jasplakinolide on the growth, encystation, and actin cytoskeleton of entamoeba histolytica and entamoeba invadens. | the effect of jasplakinolide. an actin-polymerizing and filament-stabilizing drug, on the growth, encystation, and actin cytoskeleton of entamoeba histolytica and entamoeba invadens was examined. jasplakinolide inhibited the growth of e. histolytica strain hm-1:imss and e. invadens strain ip-1 in a concentration-dependent manner, the latter being more resistant to the drug. the inhibitory effect of jasplakinolide on the growth of e. histolytica trophozoites was reversed by removal of the drug af ... | 2001 | 11318572 |
| selective 'unlabeling' of amino acids in fractionally 13c labeled proteins: an approach for stereospecific nmr assignments of ch3 groups in val and leu residues. | a novel methodology for stereospecific nmr assignments of methyl (ch3) groups of val and leu residues in fractionally 13c-labeled proteins is presented. the approach is based on selective 'unlabeling' of specific amino acids in proteins while fractionally 13c-labeling the rest. a 2d [13c-1h] hsqc spectrum recorded on such a sample is devoid of peaks belonging to the 'unlabeled' amino acid residues. such spectral simplification aids in unambiguous stereospecific assignment of diastereotopic ch3 g ... | 2001 | 11330814 |
| microbes and microbial toxins: paradigms for microbial-mucosal interactions. vi. entamoeba histolytica: parasite-host interactions. | the protozoan intestinal parasite entamoeba histolytica remains a significant cause of morbidity and mortality worldwide. e. histolytica causes two major clinical syndromes, amebic colitis and amebic liver abscess. recent advances in the development of in vitro and in vivo models of disease, new genetic approaches, the identification of key e. histolytica virulence factors, and the recognition of crucial elements of the host response to infection have led to significant insights into the pathoge ... | 2001 | 11352795 |
| intestinal amebiasis: a diagnosis not to be missed. | entamoeba histolytica is a well-recognized cause of infectious colitis and disseminated amebic abscesses. most prevalent in the tropics and subtropics, e. histolytica infections may also occur in the developed world. we describe a case of a north american traveler with intestinal amebiasis, a diagnosis first made by colonic biopsy. we review the available diagnostic tools and the role of the surgical pathologist in the detection of this infection. | 2001 | 11358014 |
| amoeboma of ascending colon with multiple amoebic liver abscesses. | a case of amoeboma of the ascending colon with multiple amoebic liver abscess is being presented; which was mistakenly diagnosed as carcinoma of ascending colon with multiple secondaries in liver. awareness of this previously unreported association is important because it adds to our knowledge of the spectrum of intestinal amoebiasis. | 2001 | 11361280 |
| amebiasis and mucosal iga antibody against the entamoeba histolytica adherence lectin in bangladeshi children. | amebiasis is the third leading parasitic cause of death worldwide, and it is not known whether immunity is acquired from a previous infection. an investigation was done to determine whether protection from intestinal infection correlated with mucosal or systemic antibody responses to the entamoeba histolytica galnac adherence lectin. e. histolytica colonization was present in 0% (0/64) of children with and 13.4% (33/246) of children without stool iga anti-galnac lectin antibodies (p= .001). chil ... | 2001 | 11372032 |
| antiprotozoal activity of the constituents of conyza filaginoides. | bioassay-guided fractionation of the antiprotozoal extract of conyza filaginoides led to the isolation of three new flavonol caffeoyl glycosides, namely, kaempferol 3-o-(6' '-o-e-caffeoyl)-beta-d-galactopyranoside (1), isorhamnetin 3-o-(6' '-o-e-caffeoyl)-beta-d-galactopyranoside (2), and quercetin 3-o-(6' '-o-e-caffeoyl)-beta-d-glucopyranoside (3). in addition, seven known compounds, erythrodiol (4), beta-caryophyllene-4,5-alpha-oxide (5), astragalin (6), isoquercitrin (7), nicotiflorin (8), na ... | 2001 | 11374974 |
| evaluation of genetic damage induced by a nitroimidazole derivative in human lymphocytes: tinidazole (tnz). | one of the useful drugs in the treatment against infestations caused by trichomonas vaginalis, entamoeba histolytica and giardia lamblia is tinidazole (tnz) 1-[2-(ethylsulfonyl) ethyl]-2-methyl-5-nitroimidazole) (gilman r.h., marquis g.s., miranda e., vestegui m., martinez h., 1988. rapid reinfection by giardia lamblia after treatment in a hyperendemic third world community. lancet i, 343-345). we decided to evaluate the potential genetic damage induced by tnz using different biological biomarke ... | 2001 | 11377093 |
| pathophysiology of amoebiasis. | few organisms are more aptly named than entamoeba histolytica, an intestinal protozoan parasite that can lyse and destroy human tissue. within the past four years, new models of e. histolytica infection have begun to illuminate how amoebic trophozoites cause intestinal disease and liver abscess, and have expanded our understanding of the remarkable killing ability of this parasite. here, i summarize recent work on the interactions between e. histolytica and human intestine, and between e. histol ... | 2001 | 11378035 |
| differential gene expression by iron-limitation in entamoeba histolytica. | | 2001 | 11378206 |
| effect of an electric field on the motility of entamoeba histolytica examined by multiple-beam interference microscopy. | a recently developed multiple-beam interference microscopic technique has been used to visualize submicroscopic structures of entamoeba histolytica and their movements in applied external electric fields. the movements were videorecorded and it was found that at low current (120 microa) pseudopods are filled with hyaline ectoplasm. at slightly higher current (about 150 microa), the amoeba stops extending the pseudopods and loosens its attachment to the surface. at higher currents (200 microa), i ... | 2001 | 11384161 |
| prevalence of viral, bacterial and parasitic enteropathogens among young children with acute diarrhoea in jeddah, saudi arabia. | the prevalence of viral, bacterial and parasitic pathogens among children of jeddah, saudi arabia, was investigated. during december 1995-october 1996, 576 faecal samples were collected from children (0-5 year(s) old) suffering from acute diarrhoea and attending hospitals and outpatient clinics in jeddah. one or more enteropathogen(s) were identified in 45.6% of the stool specimens. mixed infections were detected in 12.2% of the diarrhoeal cases. rotavirus was detected in 34.6% of the specimens ... | 2001 | 11394180 |
| [training for diagnosis of intestinal parasitic diseases in the national laboratory system of cuba]. | a national training project in the diagnosis of intestinal parasites was conducted in 1997. an initial national course was followed respectively by courses in the central, eastern, and western provinces. our results showed that cryptosporidium parvum, cyclospora cayetanensis, and leukocytes showed a significantly lower percentage of errors after the training than before (p < 0.01). the same occurred with entamoeba histolytica/e. dispar and chilomastix mesnilii (p < 0.05). among the helminths, ta ... | 2001 | 11395809 |
| disseminated acanthamoebiasis presenting as lobular panniculitis with necrotizing vasculitis in a patient with aids. | disseminated acanthamoebiasis is a rare entity, almost exclusively occurring in the immunocompromised host. | 2001 | 11401678 |
| inhibition of encystation of entamoeba invadens by wortmannin. | using an axenic encystation system in vitro, we examined the effect of wortmannin, a potent inhibitor of phosphatidylinositol 3-kinase (pi 3-kinase), which is a signaling molecule responsible for numerous cellular responses, on the encystation of entamoeba invadens. wortmannin inhibited both encystation and growth of e. invadens strain ip-1 in a dose-dependent manner, the former being more resistant to the drug than the latter. there was little decrease in the number of trophozoites after 3 days ... | 2001 | 11403379 |
| amebic liver abscess and human immunodeficiency virus infection: a report of three cases. | invasive amebiasis rarely occurs in homosexual men and human immunodeficiency virus (hiv)-infected individuals and has not been regarded as a beacon for concomitant hiv infection. we encountered a bisexual man with a protracted course of amebic liver abscess and amebic colitis. in the presence of fever, generalized lymphadenopathy, and elevated serum aminotransferase levels, hiv infection was suspected and then confirmed by a de novo seroconversion of hiv antibody. subsequently, we noted two con ... | 2001 | 11418795 |
| modifications of kohn's chlorazol black e staining and wheatley's trichrome staining for temporary wet mount and permanent preparation of entamoeba histolytica. | preparation of stained smears of entamoeba histolytica has several drawbacks. we therefore tried to simplify the staining procedures by modifing kohn's chlorazol black e staining and wheatley's trichrome staining techniques. trophozoites and cysts of axenically cultured e. histolytica and entamoeba invadens, respectively, and trophozoites and cysts of e. histolytica in stools of patients were used. karyosomes and peripheral chromatin of nuclei and chromatoid bodies became distinctly visible afte ... | 2001 | 11426739 |
| serodiagnosis of amoebiasis using a recombinant protein fragment of the 29 kda surface antigen of entamoeba histolytica. | to develop an improved serodiagnostic test for amoebiasis, we performed a detailed analysis of the immunodominant epitopes of the 29 kda surface antigen and evaluated its sensitivity and specificity. enzyme-linked immunosorbent assay (elisa) based on the fragment containing the immunodominant epitope was evaluated further and compared with full-length recombinant 29 kda protein. specificity and sensitivity of the two elisas were assessed using 55 human sera of parasitic protozoa infection cases ... | 2000 | 11428340 |
| a political asylum seeker with an amoebic empyema in birmingham. | | 2001 | 11436450 |
| purification and identification of major soluble 40-kda antigenic protein from entamoeba histolytica: its application for serodiagnosis of asymptomatic amebiasis. | one of the major soluble antigenic proteins of entamoeba histolytica was purified to homogeneity and identified on a molecular basis. its recombinant protein was expressed in escherichia coli as a fusion protein with shistosoma japonicum glutathione s-transferase. apparent molecular weight of the purified antigenic protein was estimated to be 40-kda and molecular-based analysis indicated that the purified protein was nadp+-dependent alcohol dehydrogenase (ehadh1). the application of the purified ... | 2001 | 11438429 |
| molecular cloning and characterization of peroxiredoxin from toxoplasma gondii. | a cdna of 1.1 kb comprising the gene encoding the peroxiredoxin of toxoplasma gondii (tgprx) has been cloned. the open reading frame of 591 bp was translated into a protein of 196 amino acids with a molecular mass of 25 kda. conserved 2 cysteine domains of phe-val-cys-pro and glu-val-cys-pro indicated tgprx belonged to 2-cys prx families. tgprx showed the highest homology with that of arabidopsis thaliana by 53.9% followed by entamoeba histolytica with 39.5% by the amino acid sequence alignment. ... | 2001 | 11441500 |