| nitrogen-limited behaviour of micro-organisms growing in the presence of large concentrations of ammonium ions. | cells of klebsiella pneumoniae nctc 418 grown at low culture ph values (4.5-5) in a glucose-limited chemostat culture contained elevated levels of glutamate synthase (ec 2.6.1.53). this can be taken as an indication that these cells show the physiology of nitrogen-limited cells, in spite of the fact that high concentrations (about 80 mm) of ammonium ions were present in the culture extracellular fluids. this phenomenon can be explained by the rapid diffusion of ammonia (nh3) through the cell mem ... | 1989 | 2663635 |
| trehalose dimycolate enhances resistance to infection in neutropenic animals. | bacterial infections are lethal complications of neutropenia, and antibiotics alone are inadequate therapy for these infections. irradiated mice become severely neutropenic and remain susceptible to infection for 2 to 3 weeks, depending on the dose and quality of radiation. some bacterial cell wall derivatives stimulate nonspecific host defense mechanisms against a variety of microbes which might cause postirradiation infection. in this study we determined if the cell wall glycolipid trehalose d ... | 1989 | 2663726 |
| trimethoprim resistance in enterobacteria isolated in chile. | four hundred strains of enterobacteria, isolated before the introduction of trimethoprim as a single agent in chile, were studied. one hundred and thirty six strains (34%) were resistant to greater than or equal to 8 mg/l of trimethoprim and 118 of these (87%) were also resistant to sulphamethoxazole (156 mg/l). plasmids were detected in 79% of the trimethoprim-resistant strains; 56% of the strains carried two or more plasmids. trimethoprim resistance could be transferred by conjugation in 66% o ... | 1989 | 2663804 |
| [clinical experience of chemotherapy with cefmetazole for severe infections accompanying malignant hematological disorders]. | we made an attempt to treat with cefmetazole (cmz) 25 patients who developed severe infectious diseases while suffering with granulocytopenia associated with the treatment of malignant hematological disorders. 1. determination of bacteriological efficacy while 20 strains were isolated and identified from 15 patients, no significant bacteria were detected in 9 patients. isolates obtained were: 5 strains of enterococcus faecalis, 3 strains of haemophilus influenzae, 2 strains of staphylococcus epi ... | 1989 | 2664248 |
| [susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates]. | susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ... | 1989 | 2664255 |
| construction of chimaeric promoter regions by exchange of the upstream regulatory sequences from fdhf and nif genes. | hybrid 5' regulatory regions were constructed in which the upstream activator sequence (uas) and promoter of various nif genes were exchanged with the upstream regulatory sequence (urs) of the fdhf gene from escherichia coli. they were analysed for their regulatory response under different growth conditions with the aid of fdhf'-'lacz or nif'-'lacz fusions. placement of the uas from the bradyrhizobium japonicum nifh gene in front of the spacer (dna region between urs and promoter) plus promoter ... | 1989 | 2664422 |
| the azorhizobium caulinodans nitrogen-fixation regulatory gene, nifa, is controlled by the cellular nitrogen and oxygen status. | the nucleotide sequence of the azorhizobium caulinodans ors571 nifa locus was determined and the deduced nifa amino acid sequence compared with that of nifa from other nitrogen-fixing species. highly conserved domains, including helix-turn-helix and atp-binding motifs, and specific conserved residues, such as a cluster of cysteines, were identified. the nifa 5' upstream region was found to contain dna sequence motifs highly homologous to promoter elements involved in nifa/ntr-mediated control an ... | 1989 | 2664425 |
| the q-linker: a class of interdomain sequences found in bacterial multidomain regulatory proteins. | evidence is presented that establishes a novel class of interdomain linkers, named q-linkers, as a defined element of protein structure. q-linkers occur at the boundaries of functionally distinct domains in a widespread set of bacterial regulatory and sensory transduction proteins, typified by the nitrogen regulatory proteins, ntrb, ntrc, nifa and nifl. q-linkers are not strongly conserved in sequence in otherwise homologous proteins, are approximately 15-25 residues long and relatively rich in ... | 1989 | 2664763 |
| [klebocins k6 and k150: production and activity in various experimental conditions]. | klebsiella is a common agent in hospital-acquired infections and its importance in disease transmission was evident in the isolation obtained in a health center in the city of rio cuarto. bacteriocinogenic strains by the cross-streak method in tryptic-soy agar were investigated. two klebsiella produced bacteriocins with broad patterns of sensitivity among the tested strains. the k150 klebocin was more active than the k6 klebocin, but this one was more heat-stable than k150. the klebocins were no ... | 1989 | 2664876 |
| [the effect of thiocyanate on bacterial plasmids using the plasmid screening test]. | a plasmid screening test (alkaline lysis and agarose gel electrophoresis) was used to assess the effect of thiocyanate on the plasmid replication on two bacterial strains with plasmids of different size, klebsiella pneumoniae and acinetobacter calcoaceticus. at concentrations physiologic for mammals (5-50 mg scn-/l) no effect on the replication of extrachromosomal dna was noted. this reaffirms the concept that a physiologic anion may be responsible for biological regulatory processes but will no ... | 1989 | 2665338 |
| [evaluation of a new semi-automatic method of identifying enterobacteriaceae, m.i.s.-enterobacteriaceae]. | m.i.s.-enterobacteriaceae is a new kit for identifying enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. the validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the classical method of identification in test-tubes. results showed a diagnosis accuracy of 96 p. cent at the species level and 97.7 p. cent at the genus level. diagnosis accuracy reac ... | 1989 | 2665580 |
| simulation of human serum pharmacokinetics of ticarcillin-clavulanic acid and ceftazidime in rabbits, and efficacy against experimental klebsiella pneumoniae meningitis. | the penetration into cerebrospinal fluid (csf) and efficacy of ticarcillin-clavulanic acid, ticarcillin alone, and ceftazidime were compared in rabbits with experimentally induced klebsiella pneumoniae meningitis. the compounds were administered to simulate in rabbit plasma the concentration-versus-time curves observed in humans after 30-min infusions of timentin (3 g of ticarcillin plus 100 mg of clavulanic acid), ticarcillin (3 g), and ceftazidime (2 g). single- and multiple-dosing schedules w ... | 1989 | 2665641 |
| plasmid-mediated beta-lactamases in clinical isolates of klebsiella pneumoniae and escherichia coli resistant to ceftazidime. | low-level transferable resistance to ceftazidime was detected in seven strains of klebsiella pneumoniae and one strain of escherichia coli. six of the klebsiella strains and the e. coli strain were shown to produce a novel beta-lactamase (caz-lo) with a pi of 5.6 that hydrolyzed broad-spectrum cephalosporins at low but comparable levels. one strain of k. pneumoniae was of a serotype different from that of the other strains and produced a plasmid-encoded cefuroximase (fur) with a pi of 7.5 that m ... | 1989 | 2665644 |
| studies of humoral and cell-mediated immunity to peptides shared by hla-27.1 and klebsiella pneumoniae nitrogenase in ankylosing spondylitis. | one-hundred and twenty-four patients with spondylarthropathies were studied for antibodies to the peptides from hla-b27.1 and klebsiella pneumoniae nitrogenase which share a qtdred hexamer sequence. of 60 male norwegian ankylosing spondylitis (as) patients 23.3% showed positive elisa reactivity for b27.1 peptide compared with 4% of norwegian male controls (p less than 0.10). this difference was not observed among patients and controls from new mexico. all patients with anti-b27.1 antibody were h ... | 1989 | 2665975 |
| ciprofloxacin-induced interstitial nephritis. | | 1989 | 2666013 |
| susceptibility of multidrug-resistant isolates of klebsiella pneumoniae to silver nitrate. | multidrug-resistant (mdr) clinical isolates of klebsiella pneumoniae were checked for their sensitivity toward silver nitrate by the tube-dilution method. nearly 75% of mdr strains could be successfully inhibited by 5 mg/l of silver nitrate. a significant correlation was observed between incidence of silver and trimoxazole resistance and silver and kanamycin resistance in these isolates. the genetic linkage of these two properties could not be proved since simultaneous curing and co-transfer stu ... | 1989 | 2666288 |
| effect of medium on the bacteriocin production by klebsiella pneumoniae. | the influence of growth media and media constituents on bacteriocin production by klebsiella pneumoniae was studied. among the standard laboratory media used trypticase soy broth (tsb) showed the maximum production and poor yields resulted from growth in peptone water and nutrient broth. a number of peptones differed in their bacteriocin production. best yields were observed in tryptone and proteose peptone water. addition of 1% yeast extract to tsb further stimulated bacteriocin production. how ... | 1989 | 2666289 |
| initial cloning and sequencing of hydhg, an operon homologous to ntrbc and regulating the labile hydrogenase activity in escherichia coli k-12. | to isolate genes from escherichia coli which regulate the labile hydrogenase activity, a plasmid library was used to transform hydl mutants lacking the labile hydrogenase. a single type of gene, designated hydg, was isolated. this gene also partially restored the hydrogenase activity in hydf mutants (which are defective in all hydrogenase isoenzymes), although the low hydrogenase 1 and 2 levels were not induced. therefore, hydg apparently regulates, specifically, the labile hydrogenase activity. ... | 1989 | 2666400 |
| incidence of catheter-associated gram-negative bacteremia in children with short bowel syndrome. | children with catheter-associated bacteremia were evaluated for the type of bacteria recovered and the relationship of the bacteria to the predisposing disease. a previously unrecognized observation was that gram-negative isolates, namely, escherichia coli and klebsiella sp., were almost exclusively recovered (11 of 12 isolates [92%]) from children with short bowel syndrome (sbs) compared with those from children with other underlying diseases, such as inflammatory bowel disease, malignancies, a ... | 1989 | 2666441 |
| klebsiella pneumoniae osteomyelitis: demonstration by three-phase radionuclide bone imaging. | the klebsiella pneumoniae bacillus is a rare cause of acute hematogenous osteomyelitis of long bones. bony involvement usually develops from a bacteremia associated with a klebsiella pulmonary or urinary tract infection. diabetes mellitus, alcoholism, or cirrhosis are predisposing conditions to the development of this form of osteomyelitis. a case report follows in which two sites of klebsiella osteomyelitis were demonstrated by three-phase bone imaging in a patient with both diabetes and alcoho ... | 1989 | 2666597 |
| synthetic oligonucleotide probes complementary to the hla-b variable region hybridize with klebsiella pneumoniae. | three oligonucleotide probes complementary to base sequences of the hla-b variable region were used to probe clinical and foodborne isolates of klebsiella spp. by dna colony hybridization. one oligonucleotide (rr-3) corresponding to amino acid residues 66-74 of the hla-b27.1 sequence and one corresponding to residues 66-74 of the hla-b7 sequence (rr-5) hybridized with k. pneumoniae under conditions of high stringency. a genomic library was constructed using k. pneumoniae k43 chromosomal dna, and ... | 1989 | 2666813 |
| presumptive antibiotics for penetrating abdominal wounds. | the optimal antimicrobial agent or agents for penetrating abdominal injuries remains undetermined. during the three year period ending august 1987, 317 consecutive patients undergoing celiotomy for penetrating abdominal trauma were prospectively randomized to receive either mezlocillin, 4 grams every six hours, or clindamycin, 600 milligrams every six hours, and gentamicin, loading dose of 2.0 milligrams per kilogram, then 1.5 kilograms every eight hours. antibiotics were begun in the emergency ... | 1989 | 2667180 |
| interleukin 2 as an inducer of proliferation in klebsiella-stimulated human b cells. | klebsiella membranes (klebs m), a membrane preparation from klebsiella pneumoniae, acts as a t-cell independent polyclonal b-cell activator (pba), stimulating b cells to mature into ig-secreting cells without prior proliferation. the klebs m-activated b cells express tac antigen. recombinant interleukin 2, used in costimulation with klebs m, induces proliferation and increases the ig secretion of b cells. on the other hand, klebs m-activated b cells do not respond to human bcgf. the lack of prol ... | 1989 | 2667821 |
| postpartum bacteriuria. a multicenter evaluation of different screening procedures and a controlled short-course treatment trial with amoxycillin. | a total of 10,909 puerperal women from 6 different hospitals were screened for bacteriuria by culture of voided midstream urine (msu), and a significant growth was found in 881 patients (8.1%). in 731 cases the urine was reexamined by using suprapubic aspiration (spa), and in only 354 (48%) of the samples the diagnosis of bacteriuria was confirmed. the contamination rate of the msu samples varied from 46 to 69% between the different hospitals, indicating that in the postpartum period positive ms ... | 1989 | 2668060 |
| two-dimensional gas chromatography with electron-capture detection used in the determination of specific peptidoglycan and lipopolysaccharide constituents of gram-negative bacteria in infected human urine. | gas chromatography with electron-capture detection was used in the determination of diaminopimelic acid (as the n-heptafluorobutyryl isobutyl derivative) and 3-hydroxymyristic acid (as the o-pentafluorobenzoyl methyl derivative) in gram-negative bacterial cells in infected human urine. use of the column-switching (two-dimensional gas chromatography) technique greatly enhanced the selectivity of the detection and simplified the processing of samples. the system described should prove useful for t ... | 1989 | 2668312 |
| biologically active casein peptides implicated in immunomodulation. | maternal milk should not only be considered as a nutrient, but also as a protecting agent against aggressions from the neonate's new environment. breast-feeding facilitates transmission of a passive immunity by multifunctional factors which have a direct effect on the neonate's resistance to bacterial and viral infections. among these factors are the main milk proteins, the caseins: during enzymic digestion of human and bovine caseins, immunomodulating peptides are released. corresponding synthe ... | 1989 | 2668358 |
| klebsiella pneumoniae strain k21: evidence for the rapid secretion of an unacylated form of pullulanase. | klebsiella pneumoniae strain pap996 was previously shown to secrete fatty acylated, aggregated (micellar) pullulanase only after the end of exponential growth. here we show that the closely related strain k21 secretes large amounts of unacylated, non-aggregated (monomeric) pullulanase during exponential growth. only a small amount (less than 10%) of the secreted pullulanase was initially retained by the exponentially growing cells to be subsequently secreted in a fatty acylated, aggregated form. ... | 1989 | 2668690 |
| purification and characterization of the nifn and nife gene products from azotobacter vinelandii mutant uw45. | the nifn and -e gene products are involved in the synthesis of the iron-molybdenum cofactor of dinitrogenase, the enzyme responsible for the reduction of dinitrogen to ammonia. by using the in vitro iron-molybdenum cofactor biosynthesis assay, we have followed the purification of these gene products 450-fold to greater than 95% purity. an overall recovery of 20% was obtained with the purified protein having a specific activity of 6900 units/mg of protein. the protein (hereafter referred to as ni ... | 1989 | 2668954 |
| resistance to gentamicin and amikacin of gram-negative organisms isolated from horses. | resistance of gram-negative bacteria to gentamicin has become an increasingly common problem among clinical isolates from human beings. susceptibility of isolates from horses to gentamicin and amikacin was evaluated for the period from july, 1983 to june, 1985. all isolates of escherichia coli, and species of enterobacter, klebsiella, proteus, and pseudomonas examined were susceptible to amikacin, except 2 of the 46 pseudomonas isolates. in contrast, 13 to 50% of isolates were resistant to genta ... | 1989 | 2669575 |
| shv-5, a novel shv-type beta-lactamase that hydrolyzes broad-spectrum cephalosporins and monobactams. | shv-5 (pi 8.2), a novel broad-spectrum beta-lactamase encoded by a ca. 150-kilobase plasmid, was found in klebsiella pneumoniae 160. shv-5 beta-lactamase caused decreased susceptibility to most penicillins, cephalosporins, and monobactams, except imipenem and compounds which have a c6 or c7 alpha-methoxy substituent. beta-lactamase inhibitors (clavulanic acid, sulbactam, and tazobactam) inhibited its activity and showed a synergistic effect when associated with different hydrolyzable beta-lactam ... | 1989 | 2669628 |
| replacement of potassium ions by ammonium ions in different micro-organisms grown in potassium-limited chemostat culture. | the biomass concentration extant in potassium-limited cultures of either klebsiella pneumoniae or bacillus stearothermophilus (when growing at a fixed temperature and dilution rate in a glucose/ammonium salts medium) increased progressively as the medium ph value was raised step-wise from 7.0 to 8.5. because the macromolecular composition of the organisms did not vary significantly, this increase in biomass could not be attributed to an accumulation of storage-type polymers but appeared to refle ... | 1989 | 2669673 |
| "spontaneous" pneumocephalus associated with aerobic bacteremia. | three cases of "spontaneous" pneumocephalus suspected to have resulted from aerobic bacteremia caused by enterobacter cloacae, escherichia coli, and klebsiella aerogenes are reported. in two cases, the e. cloacae and k. aerogenes were isolated from the cerebrospinal fluid. these cases were characterized by a rapid accumulation of air, without niveau, in the subarachnoid space and ventricles. | 1989 | 2670144 |
| the influence of lipopolysaccharide on the transformation efficiency of klebsiella pneumoniae. | the presence of o antigen of the lipopolysaccharide on klebsiella pneumoniae is responsible for the low transformation frequencies observed. no significant influence of capsular polysaccharide could be observed. this phenomenon was independent of the transformation procedure and plasmid size. | 1989 | 2670154 |
| prevention of clinical coliform mastitis in dairy cows by a mutant escherichia coli vaccine. | a prospective cohort study was undertaken in two commercial california dairies. the treatment group, 246 cows, received three doses of a whole cell bacterin of j5 escherichia coli (mutant of e. coli o111:b4) plus freund's incomplete adjuvant vaccine (two in the dry period and one after calving) while 240 unvaccinated cows served as controls. thirty-five cases of clinical coliform mastitis were diagnosed, six in vaccinated cows and 29 in unvaccinated cows. bacteria isolated from the clinical case ... | 1989 | 2670166 |
| importance of intestinal mucosal immunity and luminal bacterial cell wall polymers in the aetiology of inflammatory joint diseases. | the distal intestine contains bacterial cell wall polymers capable of inducing acute and chronic polyarthritis if systemically distributed. parenteral injection of peptidoglycan-polysaccharide (pg-ps) polymers from certain bacterial species produces spontaneously relapsing erosive synovitis in susceptible rat strains, and normally subarthropathic amounts of pg-ps and lipopolysaccharide (endotoxin) can reactivate arthritis initially induced by pg-ps. these experimental results illustrate the infl ... | 1989 | 2670253 |
| the relationship between klebsiella infection and ankylosing spondylitis. | ankylosing spondylitis (as) is probably produced by repeated episodes of klebsiella-reactive arthritis, usually in hla-b27-positive individuals. this concept is based on immunological, microbiological and serological considerations. immunological studies based on anti-b27 tissue typing sera and anti-b27 monoclonal antibodies indicate that hla-b27 cross-reacts with antigens found in klebsiella, salmonella, shigella and yersinia micro-organisms. salmonella, shigella and yersinia gut infections are ... | 1989 | 2670258 |
| alkaline phosphatase capture test for the rapid identification of escherichia coli and shigella species based on a specific monoclonal antibody. | a specific monoclonal antibody for escherichia coli and shigella sp. alkaline phosphatase was used in an immunocapture assay and allowed identification of e. coli either in culture isolates or directly in clinical specimens. the assay was easy and required only four steps: (i) alkaline phosphatase was released within 10 min by using a gentle lysis procedure, (ii) cell lysates were transferred to antibody-coated tubes for 45 min, (iii) p-nitrophenyl phosphate substrate was added, and (iv) alkalin ... | 1989 | 2671016 |
| third generation cephalosporins. | the third generation cephalosporins demonstrate greater potency, broader antibacterial spectrum, and more favorable pharmacologic characteristics than other cephalosporins. the majority of strains of e. coli, klebsiella pneumoniae, and proteus are susceptible, including strains resistant to aminoglycosides, anti-pseudomonas penicillins, and other cephalosporins. pseudomonas aeruginosa is susceptible to a subgroup of third generation agents including ceftazidime, cefoperazone, and the experimenta ... | 1989 | 2671141 |
| oral carriage of candida species and coliforms in patients with burning mouth syndrome. | the oral carriage of candida species and coliforms in a healthy adult population and a group of patients with burning mouth syndrome (bms) was investigated. the intra-oral prevalence of candida species and coliforms was higher in the bms group compared with the controls. the most frequent yeast isolated from the bms group was candida albicans while enterobacter and klebsiella species were the most prevalent coliforms. the possible reasons and the significance of the above findings are discussed. | 1989 | 2671340 |
| penetration of newer cephalosporins into cerebrospinal fluid. | a review of the world literature on the penetration (ratio of concentrations of cerebrospinal fluid to concentrations of serum) and attainable antibiotic concentrations in the cerebrospinal fluid in humans for cefuroxime, cefoxitin, cefotaxime, ceftizoxime, cefmenoxime, cefamandole, cefoperazone, moxalactam, ceftazidime, and ceftriaxone indicates that, with the exceptions of cefamandole and cefoperazone, all agents appear equivalent. we conclude that the choice of cephalosporin for the treatment ... | 1989 | 2672238 |
| klebsiella pneumoniae bacteremia in children. fifty-seven cases in 10 years. | a review was performed of the 57 cases of klebsiella pneumoniae bacteremia that occurred in children at our institution during a 10-year period. the rate of k pneumoniae bacteremia relative to all blood cultures in which bacteria were isolated was 1.1%. children younger than 12 months of age accounted for 38 cases (67%). there were 8 children (14%) who were afebrile at the time bacteremia was documented; other presenting clinical features were generally indistinguishable from those that characte ... | 1989 | 2672784 |
| molecular mimicry in hla-b27-related arthritis. | a unique feature of patients with ankylosing spondylitis and reactive arthritis is that almost all share the hla type b27. the primary structures of the hla-b27 antigens have been determined. at least six variants exist. however, disease predisposition does not appear to be restricted to a particular variant. one hypothesis about the pathogenesis of arthritis is that the bacteria that cause the arthritis carry components that are cross-reactive with hla-b27 antigens. several reactive bacterial c ... | 1989 | 2672931 |
| isolation and characterization of an alginate lyase from klebsiella aerogenes. | the bacterium klebsiella aerogenes (type 25) produced an inducible alginate lyase, whose major activity was located intracellularly during all growth phases. the enzyme was purified from the soluble fraction of sonicated cells by ammonium sulfate precipitation, anion- and cation-exchange chromatography and gel filtration. the apparent molecular weight of purified alginate lyase of 28,000 determined by gel filtration and of 31,600 determined by sodium dodecyl sulfate-polyacrylamide gel electropho ... | 1989 | 2673122 |
| oxidation of nitrogenase iron protein by dioxygen without inactivation could contribute to high respiration rates of azotobacter species and facilitate nitrogen fixation in other aerobic environments. | the kinetics of oxidation of the fe proteins of nitrogenases from klebsiella pneumoniae (kp2) and azotobacter chroococcum (ac2) by o2 and h2o2 have been studied by stopped-flow spectrophotometry at 23 degrees c, ph 7.4. with excess o2, one-electron oxidation of kp2 and ac2 and their 2 mgatp or 2 mgadp bound forms occurs with rate constants (k) in the range 5.3 x 10(3) m-1.s-1 to 1.6 x 10(5) m-1.s-1. a linear correlation between log k and the mid-point potentials (em) of these protein species ind ... | 1989 | 2673213 |
| [microbial contamination of water by pipe and tube materials. 3. behavior of e. coli, citrobacter freundii and klebsiella pneumoniae]. | materials water comes into contact with can promote the microbial growth as it could be shown before. the reaction of an unspecific microorganism flora and of legionella pneumophila in pipes and hoses has been described in the two previous communications. the investigation with l. pneumophila has shown that even a pathogen organism can grow upon the materials. therefore it was of special interest to prove whether indicator organisms for the testing of drinking water can grow in pipes and hoses a ... | 1989 | 2673263 |
| investigation of the structure of the capsular polysaccharide of escherichia coli k55 using klebsiella bacteriophage phi 5. | the structure of the capsular polysaccharide from escherichia coli o9:k55 (n 24c) has been studied, using methylation analysis, degradation by bacteriophage, and n.m.r. spectroscopy. depolymerisation of the k55 polysaccharide, using the lyase enzyme borne by klebsiella phi 5, yielded a tri- and a hexa-saccharide, analysis of which indicated the following repeating unit. (formula; see text) this structure differs from that for the repeating unit of the capsular polysaccharide of klebsiella k5 onl ... | 1989 | 2673507 |
| the influence of cefepime on opsonophagocytosis of bacteria. | the effect of subminimal inhibitory concentrations (sub-mics) of a semi-synthetic beta-lactam antibiotic, cefepime (bmy 28142), was studied on the opsonophagocytosis by human polymorphonuclear leukocytes (pnms) of strains of escherichia coli, klebsiella oxytoca and staphylococcus aureus. cefepime-treated gram-negative bacteria were not better phagocytosed by pmns; however the uptake of one of three s. aureus strains was enhanced. the effect of cefepime on the uptake of strains of e. coli was com ... | 1989 | 2673713 |
| identification of two novel tem-derivative ceftazidimases, caz-6 and caz-7, from klebsiella pneumoniae isolates. | | 1989 | 2674104 |
| analysis of the molecular relatedness of four extended spectrum beta-lactamases (shv-2, shv-3, shv-4 and shv-5) by comparative protein titration curves. | six beta-lactamases from klebsiella pneumoniae, five of which (shv-1, shv-2, shv-3, shv-4 and shv-5) were plasmid-encoded and one which (beta 1a gn 11-03) was chromosomally-encoded, were compared by analysis of their isoelectric points (pi), electrophoresis mobilities (mf) and titration curves or ph gradient electrophoresis. four groups were defined by their pi and mf, namely shv-1 and shv-2 (pi = 7.6, mf approximately 14), shv-3 and beta 1a gn 11-03 (pi = 7.0, mf approximately 20), shv-4 (pi = ... | 1989 | 2674105 |
| competitive inhibitors of klebsiella aerogenes urease. mechanisms of interaction with the nickel active site. | we examined several compounds for their mechanisms of inhibition with the nickel-containing active site of homogeneous klebsiella aerogenes urease. thiolate anions competitively inhibit urease and directly interact with the metallocenter, as shown by the ph dependence of inhibition and by uv-visible absorbance spectroscopic studies. cysteamine, which possesses a cationic beta-amino group, exhibited a high affinity for urease (ki = 5 microm), whereas thiolates containing anionic carboxyl groups w ... | 1989 | 2674118 |
| separation by high-performance liquid chromatography of oligosaccharides obtained after mild acid hydrolysis of klebsiella pneumoniae o1k2 (nctc 5055) lipopolysaccharides. | | 1989 | 2674176 |
| serum susceptibility of coliforms isolated from bovine intramammary infections. | a total of 35 coliforms isolated from bovine intramammary infections were tested for serum susceptibility. growth responses of klebsiella pneumoniae isolated from intramammary infections originating during the dry period were inhibited more than were growth responses of klebsiella pneumoniae isolated from infections originating during lactation. escherichia coli growth responses did not differ between isolates originating from dry period and lactational intramammary infections. mean growth respo ... | 1989 | 2674230 |
| products of defective lysogeny in serratia marcescens smg 38 and their activity against escherichia coli and other enterobacteria. | from a series of serratia marcescens clinical isolates analysed with respect to bacteriocin production, one strain (smg 38) was exceptional in that it produced two distinct phage-tail-like bacteriocins differing in morphology, sedimentation, heat sensitivity, and host range. the more active component (bc25) was effective against serratia, while the other component (mcg) inhibited growth of escherichia coli, salmonella typhimurium and shigella sonnei, but not serratia. plaque formation on tested ... | 1989 | 2674324 |
| quantitation and properties of fecal and upper small intestinal aerobic microflora in infants and young children with persistent diarrhea. | the duodenal juice and fecal aerobic microflora was investigated in 54 patients with persistent diarrhea (age less than or equal to 2 years). the duodenal aspirates yielded increased aerobic bacteria (greater than 10(5) organisms/ml) in 28 (51.9%) of the patients. established enteric pathogens were isolated from the duodenal aspirates of 12 (22.2%) of the 54 patients, viz., enteroadherent escherichia coli (eaec) (5), enterotoxigenic e. coli (etec) (3), enteropathogenic e. coli (epec) (1), nontyp ... | 1989 | 2674383 |
| comparison of sequences from the malb regions of salmonella typhimurium and enterobacter aerogenes with escherichia coli k12: a potential new regulatory site in the interoperonic region. | the male and malk genes from salmonella typhimurium, and the malefg operon and a portion of malk from enterobacter aerogenes were cloned and sequenced. plasmid-borne male genes from both species and the malf and malg genes from e. aerogenes were expressed normally in escherichia coli, and their products function in maltose transport. this shows that the malb products from the three species are interchangeable, at least in the combinations tested. the general genetic organization of the malb regi ... | 1989 | 2674653 |
| mutagenic dna repair genes on plasmids from the 'pre-antibiotic era'. | resistance transfer factors are natural conjugative plasmids encoding antibiotic resistance. some also encode mutagenic dna repair genes giving resistance to dna damage and induced mutagenesis. it has been shown that antibiotic resistance has been acquired by recent transposition events; however, we show here that mutagenic repair genes existed much earlier on these types of plasmids. conjugative plasmids from eight incompatibility groups from the murray collection of 'pre-antibiotic era' entero ... | 1989 | 2674658 |
| [in vitro and in vivo activity of a combination of clavulanic acid and amoxicillin against klebsiella pneumoniae producing extended broad-spectrum betalactamase (ctx-1, shv-3, shv-4)]. | in vitro activity of an aminopenicillin (amoxicillin or ampicillin) combined with a betalactamase inhibitor (clavulanic acid or sulbactam, 2 mg/l) was examined against 39 strains of k. pneumoniae producing 3 types of extended broad spectrum betalactamases (ctx-1, shv-3, shv-4). clavulanic acid produces the best synergistic effect. the in vivo activity of augmentin (amoxicillin + clavulanic acid), 1 g once or twice a day, during 10 +/- 3 days was evaluated on 10 cases urinary tract infections in ... | 1989 | 2674869 |
| [frequency of germ isolation from urinary infections in community practice; their sensitivity to 7 antibiotics including a combination of amoxicillin and clavulanic acid. evaluation on 1611 samples]. | a multicenter study including 10 outpatient private laboratories (hospital laboratories excluded) was carried out in france. 1,611 urines samples from patients with uti were collected during the forth trimester of 1987. the most frequently recovered pathogens were: e. coli (71%), proteus mirabilis (9%), staphylococcus coagulase (6%), klebsiella (6%), enterobacter (2%). other sorts (streptococcus d, proteus sp, pseudomonas aeruginosa, enterobacter sp) were infrequent (less than 1%). the sensitivi ... | 1989 | 2674870 |
| case report 558: multicentric klebsiella pneumoniae (friedländers bacillus) osteomyelitis in sickle cell anemia. | | 1989 | 2675323 |
| [antibiotherapy in biliary surgery. current status]. | without antibiotherapy, biliary surgery is often followed by infectious complications, possibly serious, indeed life-threatening. biliary bacteria do are responsible of these complications; mainly e. coli, streptococcus faecalis (whose pathogenicity is disputed) and klebsiella. bacteroides fragilis and pseudomonas aeruginosa are restricted to special circumstances. it is often difficult to presee whether bile is infected: some risk factors were emerged by keighley but their specificity is not ex ... | 1989 | 2675657 |
| comparative in vitro activity of a new quinolone, am-1091. | the in vitro activity of a new quinolone, am-1091 [7-(3-amino-1-pyrrolidinyl)-8-chloro-1-cyclopropyl-6-fluoro-1,4-dihydro- 4-oxo- 3-quinoline carboxylic acid hydrochloride], was compared with those of ciprofloxacin, ofloxacin, beta-lactams, and gentamicin. am-1091 inhibited 90% of the isolates of the family enterobacteriaceae at less than or equal to 0.12 micrograms/ml. for many species am-1091 was 2-fold more active than ciprofloxacin and 2- to 32-fold more active than ofloxacin. it inhibited e ... | 1989 | 2675753 |
| [antibodies to klebsiella pneumoniae in ankylosing spondylitis]. | the authors investigated the incidence of antibodies against klebsiella pneumoniae and e. coli in a group of patients with ankylosing spondylitis and in a group of healthy controls in all main immunoglobulin classes. the results revealed that in patients with ankylosing spondylitis there is a significantly higher incidence of specific antibodies of class iga against klebsiella pneumoniae and e. coli. this supports the thesis on the aetiopathogenetic interrelationship between enteric bacteria and ... | 1989 | 2676185 |
| penetration of third-generation cephalosporins into human peritoneal tissue. | each of 40 patients underwent elective laparotomy following administration of a single 1.0-gram intravenous dose of ceftizoxime, ceftriaxone, cefoperazone or cefotaxime. therapeutic concentrations of cefoperazone and ceftriaxone were achieved in peritoneal tissue in 20/20 patients. only 9/20 samples from patients receiving the other two antibiotics had detectable antibiotic activity. the antibiotic concentration in peritoneal fluid (7 samples) was 2.36-11.15 times higher than that of concurrentl ... | 1989 | 2676403 |
| potencies of ceftriaxone and cefotaxime in a single chamber pharmacokinetic system simulating their in vivo half-lives. | ceftriaxone and cefotaxime are third-generation cephalosporins with similar in vitro potencies and spectra. however, previous studies have shown that ceftriaxone had superior in vivo activity (mouse pd50 values greater than or equal to 2-fold lower) compared to cefotaxime in 23 of 46 tested enterobacteriaceae. this superior activity was thought to be due to ceftriaxone's 5- to 8-fold longer half-life. the relationship between half-life (ceftriax-one 6 h, cefotaxime 1 h) and potency was examined ... | 1989 | 2676404 |
| septic shock and bacteremia associated with laparoscopic guided liver biopsy, report on two cases. | two cases of infectious complications after liver biopsy are reported. klebsiella pneumoniae and beta-hemolytic streptococcus were cultured. in both cases the biopsy was obtained under laparoscopic control. a 28-year-old woman with liver cirrhosis died 24 hours after liver biopsy as a result of septic shock and disseminated intravascular coagulation. a 67-year-old man with hepatic fibrosis suffered from transient bacteremia and recovered uneventfully after antibiotic therapy. in these patients, ... | 1989 | 2676494 |
| mutants of the tem-1 beta-lactamase conferring resistance to ceftazidime. | spontaneous ceftazidime resistant mutants were obtained from an escherichia coli k12 j62-2 expressing the tem-1 beta-lactamase (mutation frequency = 10(-9). these mutants produced beta-lactamases with similar molecular weights, kinetic parameters and isoelectric points (pi) to the beta-lactamases produced by ceftazidime resistant clinical isolates which have recently been identified in this laboratory. mutant enzyme a focused as a doublet band at pi 5.3 with an additional weak pi 5.4 band. the d ... | 1989 | 2676935 |
| in-vitro activity of fce 22101 and other beta-lactam antibiotics against enterobacteriaceae resistant to third generation cephalosporins. | the in-vitro activity of fce 22101 was compared with those of imipenem, aztreonam, cefotaxime, ceftriaxone and latamoxef against 225 enterobacteriaceae (209 clinical isolates and 16 derivatives of escherichia coli k12) chosen for their resistance (mic greater than or equal to 8 mg/l) to third-generation cephalosporins. the beta-lactamases produced by the strains of enterobacter cloacae, klebsiella pneumoniae and esch. coli were identified. the mean mics of fce 22101 were 2-8 mg/l for ent. cloaca ... | 1989 | 2676939 |
| tigemonam activity against clinical isolates of enterobacteriaceae and enterobacteriaceae with known mechanisms of resistance to beta-lactam antibiotics. | tigemonam, a new oral monobactam, was at least as active as aztreonam or carumonam against clinical isolates of enterobacteriaceae (mic90:0.06-16 mg/l). tigemonam was very stable in the presence of classical plasmid mediated beta-lactamases but its mics were increased (4-256 mg/l) in the presence of new broad-spectrum plasmid mediated beta-lactamases (either tem of shv derivatives). increased mics (0.25-8 mg/l) were also observed for different isogenic mutants of enterobacteriaceae, which either ... | 1989 | 2676940 |
| structure-function relationships in the alpha subunit of klebsiella pneumoniae nitrogenase mofe protein from analysis of nifd mutants. | crude extracts of wild-type, nitrogenase-derepressed klebsiella pneumoniae fractionated by nondenaturing gel electrophoresis contain, in addition to the major form of the mofe protein, two minor variants of lower electrophoretic mobility. of seven nif- mutants of k. pneumoniae with nonpolar point mutations in nifd (encoding the alpha subunit of kp1), three exhibit a wild-type-like electrophoretic pattern, whereas in the remaining four, the slowest-migrating form becomes the predominant species. ... | 1989 | 2676989 |
| protein secretion by gram-negative bacteria. characterization of two membrane proteins required for pullulanase secretion by escherichia coli k-12. | pullulanase secretion in escherichia coli depends on the expression of a malt-regulated operon called pulc. characterization of the first two genes of this operon showed that they encode, respectively, a 31,000-da protein (pulc) and a 70,600-da protein (puld) which has a putative signal peptide and that these two proteins are required for pullulanase secretion. the analysis of alkaline phosphatase hybrid proteins generated by tnphoa mutagenesis of pulc and puld showed that both pulc and puld con ... | 1989 | 2677007 |
| biosynthesis and secretion of pullulanase, a lipoprotein from klebsiella aerogenes. | we constructed, by site-directed mutagenesis, a mutant pullulanase gene in which the cysteine residue in a pentapeptide sequence, leu16-leu-ser-gly-cys20 within the nh2-terminal region of pullulanase from klebsiella aerogenes, is replaced by serine (ser20). the modification, processing, and subcellular localization of the mutant pullulanase were studied. labeling studies with [3h]palmitate and immunoprecipitation with mouse antiserum raised against pullulanase showed that the wild form of both t ... | 1989 | 2677008 |
| endogenous klebsiella endophthalmitis: case report. | a case of endogenous klebsiella pneumoniae endophthalmitis, cryptogenic in origin, is presented. it was treated initially with success, only to recur three months later with loss of the eye. common sites of klebsiella infection are reviewed. this organism rapidly destroys retinal photoreceptors. a link between klebsiella and acute anterior uveitis has been suggested but is not supported by prospective data. | 1989 | 2677699 |
| [klebsiella pneumoniae resistant against 3d-generation cephalosporins]. | | 1989 | 2677797 |
| [bactericidal effect of cefotaxime-sulbactam and imipenem combined with gentamicin and/or ciprofloxacin against ctx-1 producing klebsiella pneumoniae]. | bactericidal activity of gentamicin, imipenem, ciprofloxacin and cefotaxime associated with sulbactam are studied on 36 beta lactamase ctx-1 (tem-3) producing klebsiella pneumoniae strains isolated of sputum samples and urines, among patients of intensive care units, in rangueil hospital (toulouse). gentamicin, imipenem, cefotaxime associated with sulbactam have a bactericidal activity at serum levels, obtained with usual therapeutic doses. ciprofloxacin has a bactericidal activity at concentrat ... | 1989 | 2677925 |
| [broad spectrum beta-lactamases and the api atb 244 system: the need for detection]. | api atb 24h is an automated system designed to test the sensitivity of bacteria to antibiotics. using this system we found that it was not fully able to detect acquired resistance to oxy-iminocephalosporins in enterobacteriaceae producing extended broad spectrum betalactamase (ctx-1, shv-3, shv-4). however, the frequency of detection varied with the type of api system (atb g-, atb pse), the nature of beta lactam antibiotic (cefotaxime, ceftazidime) and the type of beta lactamase produced. consid ... | 1989 | 2677926 |
| in vitro activity of the nitrogen fixation regulatory protein nifa. | we have detected activity of the nitrogen fixation regulatory protein nifa of klebsiella pneumoniae in vitro. to do so we directed synthesis of nifa in a coupled transcription-translation system and detected its ability to activate expression of a translational fusion between the nifh and lacz genes. we infer that nifa stimulates initiation of transcription by sigma 54 holoenzyme from the nifhdk promoter. the activity of nifa was lost rapidly under both aerobic and anaerobic conditions at 30 deg ... | 1989 | 2678099 |
| pulmonary disease caused by mycobacterium terrae complex. | a 64-year-old alcoholic man had a chronic cavitary pulmonary infiltrate. initial sputum smears and cultures yielded abundant acid-fast organisms subsequently identified as mycobacterium terrae. treatment with rifampin and ethambutol resulted in progressive clinical and roentgenographic resolution with an apparent cure after 18 months of therapy. | 1989 | 2678502 |
| in vitro and in vivo activities of qa-241, a new tricyclic quinolone derivative. | the in vitro susceptibilities of 1,310 clinical isolates to qa-241, a novel tricyclic quinolone, were evaluated in comparison with susceptibilities to norfloxacin, ofloxacin, enoxacin, and ciprofloxacin. the mics of qa-241 for 90% of staphylococci, enterococcus faecalis isolates, and streptococcal species ranged from 1.56 to 6.25 micrograms/ml, and the activity of qa-241 was similar to those of norfloxacin and enoxacin but two to four times less potent than those of ofloxacin and ciprofloxacin. ... | 1989 | 2679369 |
| [reciprocal effect of klebsiella pneumoniae and lactobacillus on their cytadhesion]. | reciprocal effect of 4 strains of klebsiella pneumoniae and 6 lactobacillus strains on their cytadhesion in mixed populations was studied on a model of formalinized human erythrocytes. the lactobacillus strains included 2 strains of lactobacillus casei subsp. casei, 2 strains of l. plantarum and 2 strains of l. fermentum. it was shown that adhesion of both the klebsiella and the lactobacillus strains changed under their reciprocal effect. the changes were characterized by the strain differences ... | 1989 | 2679471 |
| study on possible deleterious effects induced in white mice and rabbits by prolonged administration of polidin in different concentrations. | there were investigated possible deleterious effects induced in white mice and rabbits by prolonged administration of polidin in different concentrations, as well as of polidin associated with membrane fraction of klebsiella pneumoniae. in the experimental model used all the animals evolved normally presenting some transient blood modifications which occurred after administration of comparatively much higher doses than those usually administered in man. there were not noticed significant macrosc ... | 1989 | 2679488 |
| a double-blind controlled trial of norfloxacin versus cotrimoxazole in the treatment of urinary tract infections. | a double-blind controlled trial of norfloxacin versus cotrimoxazole in the treatment of urinary tract infections was conducted. eighty-eight patients were recruited but data from 80 patients were analysed. norfloxacin cured 93 per cent of the infections while the cure rate achieved by cotrimoxazole was only 70 per cent (p = 0.03, fisher's exact test). the difference was attributable to a higher incidence of resistance to cotrimoxazole among the bacterial isolates. escheria coli was the commonest ... | 1989 | 2679836 |
| [epidemiology of klebsiella pneumoniae infections in 2 colonies of squirrel monkeys and lemurs]. | after a short summary of the occurrence, sources, phenotypic characteristics, epidemiologic markers, virulence factors and pathogenicity of the 7 species of klebsiella, the author reported (1) an infection due to k. pneumoniae k5 in a breed of squirrel monkey. these animals were suffering from sub-cutaneous abcesses (pasteur institute of cayenne, french guyana). (2) the second example refers a fatal infection due to k. pneumoniae k2 in a colony of lemurs, at mulhouse zoological garden (east of f ... | 1989 | 2680130 |
| klebsiella pneumoniae and hla b27-associated diseases of reiter's syndrome and ankylosing spondylitis. | | 1989 | 2680296 |
| yersinia pseudotuberculosis infection as a cause of reactive arthritis as seen in a genitourinary clinic: case report. | patients are often referred to the genitourinary clinic for screening for urethral infections after a clinical diagnosis of reiter's syndrome or reactive arthritis. we report a case of reactive polyarthritis in which serological evidence of yersinia pseudotuberculosis infection was found in the absence of the other common precipitating organisms, such as chlamydia spp, klebsiella spp, shigella spp, campylobacter spp, or yersinia enterocolitica. | 1989 | 2680895 |
| reduction of capsular polysaccharide production in klebsiella pneumoniae by sodium salicylate. | heavily encapsulated klebsiella pneumoniae (serotypes 1 and 2) was cultured in the presence of sodium salicylate. the addition of salicylate (2 to 30 micrograms/ml) progressively decreased the amount of capsular polysaccharide produced by all strains without significantly inhibiting cell growth. further addition of salicylate (50 to 200 micrograms/ml) was progressively inhibitory to cell growth and decreased the production of polysaccharide only slightly. the optimal concentration of salicylate ... | 1989 | 2680983 |
| cause, occurrence, and clinical signs of mastitis and anorexia in cows in a wisconsin study. | microbiologic culture revealed the following cause of mastitis and anorexia in 145 cows in wisconsin to be escherichia coli, 66 cows; klebsiella spp, 3; corynebacterium pyogenes, 27; streptococci, 21; staphylococci, 20; yeasts, 1; and no bacterial growth, 7. mastitis was detected with approximately equal frequency throughout the year. escherichia coli was isolated throughout the year, but was more common and was the predominant organism during the summer. corynebacterium pyogenes was isolated mo ... | 1989 | 2681108 |
| in-vitro activity of meropenem against clinical isolates in a multicentre study in italy. | a multicentre in-vitro study was undertaken to evaluate the susceptibility of bacterial pathogens isolated in different italian hospitals to meropenem. a total of 1399 aerobic and 452 anaerobic strains was analysed. comparative agents were imipenem, cefotaxime, ceftazidime, ceftriaxone, piperacillin, ciprofloxacin, gentamicin, amikacin, plus vancomycin when appropriate. the mic ranges (mg/l) of meropenem were: 0.015-2 for klebsiella spp., proteus spp., morganella morganii and providencia spp.; l ... | 1989 | 2681128 |
| epidemic of serratia marcescens bacteremia in a cardiac intensive care unit. | from 16 july through 27 september 1988, seven cases of nosocomial serratia marcescens bacteremia occurred in a cardiac care unit. in all seven case patients, s. marcescens was isolated from blood cultures. two of the seven had other microorganisms identified in the blood culture in which s. marcescens was recovered; one had enterobacter cloacae, and one had klebsiella pneumoniae. a case-control study was conducted to identify risk factors for bloodstream infection. case patients were more likely ... | 1989 | 2681247 |
| colonization of neonates in a nursery ward with enteropathogenic escherichia coli and correlation to the clinical histories of the children. | stool samples were examined from 30 preterm neonates admitted to a nursery ward; 16 neonates had diarrhea, 12 constituted an age-matched control group without diarrhea, and 2 had an unknown history regarding diarrhea. variable numbers of enteropathogenic escherichia coli serotype o111:hnt strains possessing the gene coding for the enteroadherence factor (eaf) were found in stool samples from 13 of the neonates. no other microbiological enteropathogen was found. a total of 294 strains (9 or 10 fr ... | 1989 | 2681255 |
| preservation of myocardial ultrastructure after 24 hours of klebsiella sepsis: histologic, functional, and biochemical correlations. | myocardial function with ultrastructure and high energy phosphate levels in dogs was correlated after 24 hours of sepsis using live klebsiella aerogenes. all animals developed progressive hemodynamic deterioration over a 24 hour period. mean arterial pressure decreased from 148 +/- 7 mmhg to 85 (p less than 0.01) and cardiac output decreased from 3.43 +/- .31 to 1.6 +/- 0.5 l/min. left ventricular stroke work decreased from 48.2 +/- 5 to 18.1 +/- 6 gm-meters (p less than 0.001). systemic and pul ... | 1989 | 2681798 |
| [duration of the preventive use of antibiotics in colorectal surgery--single administration versus short-term prevention]. | the effect of a combination of 4 g mezlocillin and 0.5 g metronidazole for the prophylaxis against infections in a one-shot dose immediately preoperatively compared to a short-time dose of 2 days given to 90 patients with resection of colorectal carcinoma was investigated in a prospective and randomized study. 6 patients developed a wound infection in the early postoperative phase; 4 of these infections (3 were severe, 1 was mild) occurred in the one-shot group and 2 in the short-time prophylaxi ... | 1989 | 2682096 |
| cefixime versus trimethoprim/sulfamethoxazole in treatment of patients with acute, uncomplicated lower urinary tract infections. | one hundred six patients with acute, uncomplicated lower urinary tract infections participated in a randomized study that compared cefixime (one 400-mg tablet once daily) with trimethoprim (160 mg)/sulfamethoxazole (800 mg) (one tablet every 12 hours). two cefixime recipients and 3 patients given trimethoprim/sulfamethoxazole had courses that were not evaluable for efficacy. at five to nine days post-therapy, 98 percent of the patients in each treatment group had clinical cure and bacteriologic ... | 1989 | 2683326 |
| phase ii study of d.651, an oral vaccine designed to prevent recurrences of vulvovaginal candidiasis. | a vaccine has been prepared with ribosomes of candida albicans serotypes a and b plus, as adjuvant, membrane proteoglycan from a nonencapsulated klebsiella pneumoniae. a preliminary phase ii trial without placebo control was conducted in 22 women with a history of frequent recurrences of vulvovaginal candidiasis (vvc). initially, all the patients were treated locally with an antimycotic cream. beginning at the same time, vaccine was taken orally in capsules containing 0.55 microgram active compo ... | 1989 | 2683457 |
| infected ventriculostomy: bacteriology and treatment. | during a period of 6 years 256 patients underwent ventriculostomy as their first operative procedure. twenty-seven patients (11%) developed csf-infections, in almost 90% caused by gram-positive cocci compatible with normal skin flora. the incidence of infection was distributed evenly in the postoperative period. all patients received intravenous and intrathecal antibiotic treatment simultaneously according to the sensitivity test. the treatment was highly effective, and without serious adverse e ... | 1989 | 2683602 |
| novel plasmid-mediated beta-lactamase (tem-10) conferring selective resistance to ceftazidime and aztreonam in clinical isolates of klebsiella pneumoniae. | two clinical isolates of klebsiella pneumoniae from seriously ill patients in chicago, ill., have been identified as resistant to ceftazidime and aztreonam but susceptible to other cephalosporins. this unusual antibiogram was shown to be due to a novel plasmid-mediated beta-lactamase which readily hydrolyzed ceftazidime and aztreonam in addition to penicillins such as piperacillin and carbenicillin. this enzyme and its attendant resistance were transferred to escherichia coli by conjugation on a ... | 1989 | 2684007 |
| transcriptional activation of the klebsiella pneumoniae nifla promoter by ntrc is face-of-the-helix dependent and the activator stabilizes the interaction of sigma 54-rna polymerase with the promoter. | activation of transcription at the klebsiella pneumoniae nifla promoter requires the phosphorylated form of the positive control protein ntrc, together with rna polymerase modified by the alternative sigma factor sigma 54. dimethylsulphate and potassium permanganate were used as probes to analyse the interaction of ntrc and sigma 54-rna polymerase with supercoiled nifla promoter dna in vitro. in contrast to the glnap2 promoter, sigma 54 holoenzyme did not protect guanine residues in the nifla pr ... | 1989 | 2684643 |
| cadmium resistance in some members of enterobacteriaceae. | strains of members of enterobacteriaceae, namely escherichia coli (18), klebsiella aerogenes (16), and serratia marcescens (16) were screened for cd resistance or sensitivity. only one strain each of these was resistant to high levels (25 n moles/0.05 ml) cdcl2. the minimal inhibitory concentration (mic) of sensitive strains ranged from 0.8-5 micrograms/ml. all the resistant strains were simultaneously resistant to a number of antibiotics. treatment with sodium dodecyl sulfate eliminated resista ... | 1989 | 2684849 |
| asymptomatic bacteriuria in school children. | we studied 2447 asymptomatic school children by microscopic examination of urine and culture of a clear voided midstream specimen of urine. only three children had bacteriuria (0.12%). urographic abnormalities were detected in both the cases, which could be investigated. this study has shown that asymptomatic bacteriuria is comparatively rare in our community; however where there is bacteriuria, renal damage is common. | 1989 | 2684850 |
| klebsiella pneumoniae osteomyelitis in sickle cell anemia. | a case of klebsiella pneumoniae osteomyelitis in a patient with sickle cell anemia is reported. the literature is briefly reviewed. the case is presented because of the rarity of klebsiella pneumonia osteomyelitis in sickle cell disease. | 1989 | 2684851 |
| in vivo studies on the interaction of rna polymerase-sigma 54 with the klebsiella pneumoniae and rhizobium meliloti nifh promoters. the role of nifa in the formation of an open promoter complex. | transcription from the klebsiella pneumoniae and rhizobium meliloti nifh promoters requires the positive control protein nifa and the alternative sigma factor sigma 54, encoded by the rpon gene. transcription from the k. pneumoniae nifh promoter is fully dependent upon nifa bound at the upstream activator sequence (uas) whereas the r. meliloti nifh promoter can be efficiently activated in the absence of this sequence and can also be activated by a mutant form of nifa unable to bind the uas. the ... | 1989 | 2685331 |