| spike-and-wave neocortical patterns in rats: genetic and aminergic control. | spontaneously occurring and drug-induced high voltage spike-and-wave electroencephalogram patterns were examined in inbred rats of the fischer 344 and buffalo strains and of the random-bred sprague-dawley strain at different ages. in addition, tyrosine hydroxylase activity and dopamine d2 receptor density were determined in the substantia nigra, corpus striatum, olfactory tubercle and ponsmedulla areas of fisher 344 and buffalo animals. high voltage spike-and-wave episodes were present in 87.5% ... | 1990 | 2263319 |
| analysis of interleukin 2 and various effector cell populations in adoptive immunotherapy of 9l rat gliosarcoma: allogeneic cytotoxic t lymphocytes prevent tumor take. | recombinant interleukin 2 (ril-2) and various effector cell populations were used for adoptive immunotherapy in the fischer strain 9l rat gliosarcoma model. the in vivo cytotoxicities of nonspecifically activated lymphocytes and specifically activated cytotoxic t lymphocytes (ctls) were assessed in a modified in vivo neutralization (winn) assay. effector cells (10(6)) and 9l tumor cells (10(5] were combined with 10(4) units of ril-2 and stereotactically implanted into the right frontal centrum s ... | 1990 | 2263613 |
| [guillain-barré syndrome. 15 pediatric cases]. | clinical features and outcome of 15 children with guillain-barré syndrome were retrospectively analyzed. in 53% of the patients there was an infectious illness previous to the neurologic symptoms and in five children the etiologic agent was demonstrated. motor deficit affecting limbs was the most common clinical presentation and facilal paralysis was the most frequent accompanying sign. one of these children developed the fisher syndrome variant. the outcome was excellent except in one case. we ... | 1990 | 2275491 |
| no difference in expression of chromosomal fragile sites in patients with solid malignant tumours and normal controls. | the frequency and distribution of rare and common chromosomal fragile sites in metaphases derived from peripheral lymphocytes were compared in 26 patients with malignant solid tumours and 24 normal controls. in order to avoid bias in evaluation, the identity of each individual as patient or control was disclosed only after the study was completed. rare heritable folic acid inducible fragile sites were found in five patients (2q13; 6p23; 8q22; 16p12) and two controls (8q22). common fragile sites ... | 1990 | 2278966 |
| spiral ganglion cell endings in the cochlear nucleus of young and old rats. | the spiral ganglion cells (sgcs) forming the auditory nerve have been shown to degenerate with age in both human and animal models, presumably resulting in post-synaptic sites in the cochlear nucleus (cn) that have lost their inputs. the present study examined the morphological changes in the surviving sgc endings in the cn of aged animals. the auditory nerves of 2-3 mo and 25-26 mo male, fisher 344 rats were anterogradely labeled with horseradish peroxidase and the cn prepared histochemically. ... | 1990 | 2292496 |
| [morphological changes in fetal small intestine and pancreas implanted in fisher and august rats]. | fragments of fetal small intestine of fischer rats aged 16-20 days were implanted to rats aged 3 months of the same fischer strain (syngeneic implantation). the morphological changes in the implants were investigated. it has been shown that serious modifications of the implant including the neoplasm formation are possible during the evolution process. after syngeneic implantation of fetal pancreas to fischer and august rats under identical conditions no malignization of the implants was observed ... | 1990 | 2298141 |
| continuous nimodipine treatment attenuates cortical infarction in rats subjected to 24 hours of focal cerebral ischemia. | focal cerebral infarction and edema were measured in rats (wistar, fisher 344, and spontaneously hypertensive strains) pretreated with nimodipine (2 micrograms/kg/min i.v.) or its vehicle and subjected to the tandem occlusion of the middle cerebral and common carotid arteries. animals awoke from anesthesia 10-15 min after onset of ischemia and continued to receive treatment over a 24-h survival period. cortical infarction and edema were quantified by image analysis of frozen brain sections proce ... | 1990 | 2298839 |
| enhancement of pancreatic and hepatic glutathione levels in rats during cyanohydroxybutene intoxication. | 1-cyano-2-hydroxy-3-butene (chb), a cruciferous plant product, is hepatotoxic, pancreatotoxic, and elevates glutathione (gsh) in liver and pancreas. whether gsh elevation is preceded by a depletion related to toxic insult, or whether toxicity and gsh elevation are unrelated, is not known. to evaluate the temporal relationship between toxicity and gsh levels, male fisher 344 rats (6/group) were given chb (200 mg/kg po) and killed up to 96 hr after dosing. at death, histological and ultrastructura ... | 1990 | 2307313 |
| effects of 1,3-dichloropropene on the kidney of fisher 344 rats after pretreatment with diethyl maleate, buthionine sulfoximine, and aminooxyacetic acid. | acute nephrotoxicity of cis/trans-1,3-dichloropropene (dcp) was assessed in male fisher 344 rats. pretreatment of rats with corn oil, aminooxyacetic acid (aoa), buthionine sulfoximine (bso), or diethyl maleate (dem) was given intraperitoneally 1 h or 4 h prior to injection of dcp. doses of dcp were 0, 25, 50, and 75 mg/kg intraperitoneally (4-5 animals per dose/pretreatment group). urine was collected for 24 h. excretion of creatinine, phosphorus, protein, n-acetylglucosaminidase (nag), and the ... | 1990 | 2313738 |
| a model of reversible obstructive jaundice in the rat. | a model of reversible, extrahepatic biliary obstruction is described. vessel loop blockade of the biliary tree results in obstructive jaundice while removal of the exteriorized vessel loop provides internal biliary drainage without subsequent laparotomy. this technique combined with a system for chronic venous infusion and arterial blood sampling in the unrestrained rat is ideal for long-term metabolic studies of obstructive jaundice. male fisher 344 rats (275-350 g) underwent either the combine ... | 1990 | 2314093 |
| effect of aging on intestinal biotin transport in the rat. | the influence of aging of biotin intestinal transport was examined in fisher 344 rats (3- 12- and 24-month old) using a brush border membrane vesicle (bbmv) technique. in all age groups examined, transport of biotin as a function of concentration was saturable in the presence of a na+ gradient (out greater than in), but was lower and linear in the presence of a choline gradient (out greater than in.). the vmax of the biotin transport process was found to be significantly (p less than 0.01) highe ... | 1990 | 2318284 |
| a second thyroglobulin messenger rna species (rtg-2) in rat thyrocytes. | a 0.95-kilobase (kb) thyrocyte rna, initially detected in our 1b-6 subclone of fisher rat thyrocytes (frtl-5) using an oligonucleotide probe complementary to the 5' end of rat thyroglobulin (tg) mrna, was also detected in cultured thyrocytes of the wistar rat (wrt cells) and in freshly isolated normal rat thyroid tissue. this transcript was thyroid specific and as abundant as the previously characterized 9.0-kb tg mrna in the cultured thyroid cells under the growth conditions employed. the small ... | 1990 | 2325666 |
| genitalia in human figure drawings: childrearing practices and child sexual abuse. | to replicate and explore the associations of drawing genitalia on a human figure, child-rearing practices, and a history of alleged sexual abuse, we designed a cross-sectional study of 109 alleged child sexual abuse victims, ages 3 through 8 years, and a group of 109 comparison children matched for age, sex, race, and socioeconomic status but with no history of abuse. a standardized format was used to collect drawings, administer the peabody picture vocabulary test, and gather background data on ... | 1990 | 2329432 |
| evidence that cyclosporine prevents rejection and recurrent diabetes in pancreatic transplants in the bb rat. | cyclosporine prevents the development of diabetes in spontaneously diabetic bb rats and nod mice. however, islet transplants have been shown to be subject to immunologic destruction in hosts treated with csa and anti-cd4 antibody or those rendered tolerant to donor antigens. this study determines whether the minimum dose of csa necessary to prevent rejection of pancreatic transplants will also prevent recurrent diabetes in pancreas transplants in bb rats. lewis recipients promptly reject bn (n = ... | 1990 | 2336700 |
| mri characterization of 9l-glioma in rat brain at 4.7 tesla. | in vivo estimation of intracranial tumor progression is important in tumor treatment response studies in animal models. high resolution mr images at 4.7 t of 9l-gliomas stereotactically implanted in fisher-344 rat brains were obtained. due to elongation of t1 at higher fields, tissue contrast is diminished in t1-weighted images. however, normal anatomy and vasogenic edema are clearly discerned in t2-weighted images (echo times of greater than 50 ms and recycle times of greater than 2 sec). tumor ... | 1990 | 2338899 |
| loss of muscarinic m1 receptors with aging in the cerebral cortex of fisher 344 rats. | age-related changes in central cholinergic muscarinic receptors were measured in young (3-6 month), middle-aged (15-17 month), and aged (22-26 month) male fisher 344 rats by receptor binding techniques. using [3h]-quinuclidinyl benzilate as the ligand, a significant decrease (14-19%) in the number of muscarinic cortical receptors was measured in aged rats compared to both young and middle-aged rats. with the selective m1 antagonist, [3h]-pirenzepine, a 17% decrease in receptor density was observ ... | 1990 | 2339151 |
| dose-response relationship between o6-methylguanine formation in clara cells and induction of pulmonary neoplasia in the rat by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. | the relationship between the formation of o6-methylguanine (o6mg) and the induction of lung, liver, and nasal tumors in the fisher 344 rat by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) was examined in a dose-response study. animals were treated for 20 wk (3 times/wk) with concentrations of nnk ranging from 0.03 to 50 mg/kg to induce tumors. steady-state concentrations of o6mg were quantitated, and cytotoxicity was assessed in target cells and tissues af ... | 1990 | 2340522 |
| glutathione s-transferase subunit induction patterns of brussels sprouts, allyl isothiocyanate and goitrin in rat liver and small intestinal mucosa: a new approach for the identification of inducing xenobiotics. | effects of brussels sprouts (2.5-30%), allyl isothiocyanate (0.03 and 0.1%) and goitrin (0.02%), in the diet, on the glutathione s-transferase subunit pattern in the liver and small intestinal mucosa of male fisher rats were investigated. a statistically significant linear relationship was found between the amount of brussels sprouts in the diet and the induction of glutathione s-transferase subunits in two experiments. increases in total activity of glutathione s-transferases towards 1-chloro-2 ... | 1990 | 2341092 |
| regrowth of damaged neurosecretory axons to fenestrated vessels of implanted peripheral tissues. | a major target of neurosecretory axons (nsa) is the basal lamina around fenestrated blood vessels (fbv) in the neural lobe of the pituitary gland. we have posed the question of whether there is neurovascular specificity. do mature, regenerating nsa terminate selectively on the fbv of the neural lobe compared with the fbv of other tissues that normally are not innervated by nsa? three types of tissue were transplanted between inbred fisher rats. fragments, about 1 mm3, of pineal, adrenal medulla, ... | 1990 | 2343374 |
| root morphology of mandibular premolars in human 45,x females. | the teeth of 87 finnish 45,x females, 60 of their first-degree relatives and 87 normal females were examined. the presence of separate mesial and distal root canals and separate root apices was noted from orthopantomograms. of the 45,x females, 40 (or 46%) had lower premolars with separate root canals; 30 individuals in first and 28 in second premolars. the number of first and second premolars with separate mesial and distal root canals was 40 and 43, and of those also showing separate apices, 2 ... | 1990 | 2344286 |
| in vivo dental plaque-forming ability and cariogenicity of the bacterium streptococcus bovis in gnotobiotic rats. | germ-free fisher rats, fed on a high sucrose diet, were monoinfected with streptococcus bovis strains. high levels of fissure caries were formed by three strains; one strain produced moderate levels; another produced only very low levels. no strain could produce approximal or buccal/lingual lesions. all strains could colonize the fissures of teeth in the lower jaw, as shown by scanning electron microscopy. the most cariogenic strains were associated with caries scores similar to those of strep. ... | 1990 | 2344292 |
| proteoglycans synthesized by human glomerular mesangial cells in culture. | human fetal kidney mesangial cells were cultured for 24 h in the presence of 3h-amino acids and [35s] sulfate and chased for 24 h in nonradioactive medium. incubation medium and cell layer proteoglycans were purified twice by high performance liquid chromatography-deae chromatography followed by gel filtration chromatography. the major medium 35s-macromolecules were chondroitin/dermatan-35so4 proteoglycans. a small, sepharose cl-6b kav 0.14 dermatan-35so4 proteoglycan was detected in the labelin ... | 1990 | 2345181 |
| substrate alterations in a sarcoma-bearing rat model: effect of tumor growth and resection. | the time course of plasma substrate level alterations in the tumor-bearing (tb) host and their relationship to tumor burden or weight loss has not been well defined. plasma glucose, lactate, pyruvate, cholesterol, triglycerides, acetoacetate, beta-hydroxy butyrate, and glycerol levels were measured in postabsorptive tb and nontumor-bearing (ntb) male fisher 344 rats at weekly intervals for 5 weeks. chronically starved rts (cs) had plasma substrates measured at week 3 and compared with tb and ntb ... | 1990 | 2352423 |
| chimeric analysis of the site of lurcher gene action with respect to glial enzyme expression. | immunocytochemical analysis of aggregation chimeras made using either of the mutants, lurcher or purkinje cell degeneration, previously showed that only bergmann glia close to surviving purkinje cells expressed an apparently normal level of the enzyme, glycerol-3-phosphate dehydrogenase (gpdh) (fisher and mullen, 1988). in the present study, aggregation chimeras were made using embryos from a b6d2 hybrid mouse strain carrying the lurcher (lc/+) mutation and homozygous for an allele specifying a ... | 1990 | 2358967 |
| role of calcium in adrenergic neurochemical transmission in the aging heart. | the effect of modification of extracellular ca2+ concentration ([ca2+]0), the administration of the neuronal ca2+ channel blocker, omega-conotoxin, and the calcium (ca2+) ionophore, ionomycin, were examined in the isolated heart of male fisher-344 rats 6 and 24 months of age. hearts with the right cardiac sympathetic nerve intact were isolated and perfused with krebs-ringer solution containing cocaine (10(-6) m) to block uptake 1, metanephrine (10(-6) m) to block uptake 2 and yohimbine (3 x 10(- ... | 1990 | 2359031 |
| pancreatic hepatocytes in fischer and wistar rats induced by repeated injections of cadmium chloride. | the effects of multiple sc injections of cadmium chloride (cdcl2) on the pancreas of two rat strains (wistar [wf/ncr] and fischer [f344/ncr]) were studied by histological and immunohistochemical examinations. a high incidence of hepatocytic foci occurred within the pancreata of both strains that was associated with this cadmium exposure. although pancreatic hepatocytes (ph) were found in both strains, wistar rats were more susceptible to cadmium-induced ph formation while showing more tolerance ... | 1990 | 2360204 |
| analysis of capillary geometry in rat subepicardium and subendocardium. | the sustained high-energy turnover of cardiac muscle presents a formidable challenge to the o2 delivery systems. one major determinant of blood-tissue gas exchange potential is capillary surface area per volume of muscle fiber, sv(c,f). estimation of sv(c,f) necessitates quantification of capillary orientation. capillary geometry was analyzed systematically in subepi- (epi) and subendocardium (endo) of glutaraldehyde perfusion-fixed rat heart (n = 4). on 1-micron sections cut rigorously transver ... | 1990 | 2375407 |
| exogenous nerve growth factor increases the activity of high-affinity choline uptake and choline acetyltransferase in brain of fisher 344 male rats. | the objective of this study was to determine the effect of age and chronic intracerebral administration of nerve growth factor (ngf) on the activity of the presynaptic cholinergic neuronal markers hemicholinium-sensitive high-affinity choline uptake (hacu) and choline acetyltransferase (chat) in the brain of fisher 344 male rats. in 24-month-old rats, a substantial decrease in chat activity (30%) was measured in striatum, and decreases in hacu were found in frontal cortex (28%) and hippocampus ( ... | 1990 | 2384747 |
| miller fisher syndrome associated with q fever. | a 51 year old woman with pneumonia developed miller fisher syndrome. indirect immunofluorescence tests showed antibodies against coxiella burnetti. miller fisher syndrome associated with q fever has not been described previously. | 1990 | 2391529 |
| mutational activation of the cellular harvey ras oncogene in rat esophageal papillomas induced by methylbenzylnitrosamine. | epidemiological studies have demonstrated a strong association between human esophageal cancer and exposure to n-nitroso carcinogens. esophageal tumors can be induced in experimental animals, especially in rats, by many n-nitroso carcinogens. in the present study, rat esophageal tumors induced by methylbenzylnitrosamine (mbna) and mbna-transformed esophageal cell lines were analyzed for activated protooncogenes. dnas from four fisher 344 rat esophageal papillomas were examined for their ability ... | 1990 | 2406014 |
| somatic antigens of pseudomonas aeruginosa. the structure of the o-specific polysaccharide chains of lipopolysaccharides of p. aeruginosa serogroup o4 (lányi) and related serotype o6 (habs) and immunotype 1 (fisher). | acidic o-specific polysaccharides were isolated on mild acidic degradation of lipopolysaccharides of pseudomonas aeruginosa serotypes o4a,b, o4a,c, o4a,d (lányi classification) and serologically related to them serotype o6 (habs classification) and immunotype 1 (fisher classification). the polysaccharides had identical monosaccharide composition and were built up of l-rhamnose, 2-acetamido-2,6-dideoxy-d-glucose,2-formamido-2-deoxy-d-galacturonic acid and 2-acetamido-2-deoxy-d-galactouronamide re ... | 1985 | 2410275 |
| effects of interferon on differentiation of normal and tumor cells. | as described in this review, both partially purified and recombinant interferons are potent modulators of differentiation in diverse cell culture systems (table 2). depending on the target cell, interferon exerts either an inhibitory or an inductive effect on cell differentiation. in certain myeloid leukemic cells, such as hl-60, interferon by itself is growth suppressive but does not induce cell maturation, whereas in combination with inducers of differentiation, such as dmso, tpa or retinoic a ... | 1985 | 2412243 |
| rat lung macrophage tumor cytotoxin production: impairment by chronic in vivo cigarette smoke exposure. | macrophages in the presence of bacteria-derived lipopolysaccharide (lps) stimuli produce a soluble cytotoxin which is toxic to tumor cells. in this study, we examined various parameters of cytotoxin production from pulmonary lavage cells obtained from fisher 344 cesarean-derived rats. cultures of macrophages were derived from pulmonary lavage cells and stimulated in vitro with lps. cytotoxin production was assayed in vitro using an l-929 cell target assay. pulmonary lavage preparations contained ... | 1985 | 2413988 |
| characteristics of normal rat mammary epithelial cells and n-ethyl-n-nitrosourea-induced mammary adenocarcinoma cells grown in culture. | the characteristics of normal mammary epithelial cells derived from lewis and sprague-dawley rats and n-ethyl-n-nitrosourea (enu)-induced mammary gland adenocarcinoma cells derived from sprague-dawley (cd) and fisher (cdf) rats and grown in culture were compared. after collagenase treatment, the rat mammary epithelial cell aggregates were placed in a hormone-supplemented medium. the normal mammary epithelial cells (ne) attached to the surface of the dish within 50 hours, whereas the mammary aden ... | 1985 | 2415045 |
| friend virus-specific cytotoxic t lymphocytes recognize both gag and env gene-encoded specificities. | we have constructed a series of "synthetic" target cell lines for an analysis of the specificity of anti-friend virus (fv) ctl. our results show that murine h-2 genes and individual retroviral genes can be stable expressed in fisher rat embryo (fre) cells, and that their products have the potential to form target structures recognized by mouse ctl. cells expressing h-2db and either the env or gag genes of one component of fv, helper friend murine leukemia virus (fmulv), were lysed by anti-fv ctl ... | 1986 | 2425028 |
| studies on high-affinity [3h]substance p binding sites in bovine pineal gland. | the presence of substance p in numerous mammalian pineal glands prompted us to search for its binding sites in the bovine pineal gland. the binding assays to pineal membrane were carried out in polypropylene microcentrifuge tubes in a final volume of 500 microliters of 50 mm tris-hcl buffer (ph 7.4) containing aliquots of 200-500 micrograms protein, 0.02% bsa, 6 micrograms/ml chymostatin, 4 micrograms/ml leupeptin, 40 micrograms/ml bacitracin, 5 mm mncl2, and 50 microliters of [3h]substance p (3 ... | 1986 | 2430788 |
| growing familiarity with the test situation augments the vigilance deficit of aged rats. | quantitative eeg profiles of aged and of young-adult fisher-344 rats were studied over 10 consecutive recording (3 h) sessions. the individual recording sessions were spaced regularly over a 6-week period. in the aged rats, in recordings from both the frontal cortex and dorsal hippocampus, the eeg characteristics of a vigilance deficit state increased in prominence up through 8 recording sessions before a stable level was reached. a similar trend was not seen in the young-adult rats; instead, th ... | 1987 | 2431885 |
| immunochemical studies of human erythropoietin using site-specific anti-peptide antibodies. identification of a functional domain. | anti-peptide antibodies that bind to the amino terminus of human erythropoietin (residues 1-26) do not inhibit the hormone's biological activity, indicating that this region of the protein does not play a role in receptor recognition (sytkowski, a. j., and fisher, j. w. (1985) j. biol chem. 260, 14727-14731). we have now identified six other regions of the primary sequence that are relatively hydrophilic and, therefore, have a higher probability of being accessible to such antibody probes. antib ... | 1987 | 2433274 |
| effects of chemistry, manufacturing and concentration of the dye basic fuchsin regarding its use in quantitative cytophotometry. | four separately manufactured preparations of basic fuchsin were compared to determine the effects of concentration, chemistry, and the manufacturing process for their quantitative value in the nuclear feulgen reaction. in order to make the necessary comparisons, the two wavelength method of quantitative cytophotometry was employed to analyse each stain application regarding dna measurements. chicken erythrocytes, and myxamoebae and plasmodia of didymium iridis were employed as experimental tissu ... | 1987 | 2438091 |
| reversing the detrimental effect of adriamycin on skin grafts in rats. | we evaluated in a rat model the effects of a homologous fibrin glue in reversing the effects of adriamycin on adherence and take of skin grafts. a total of 40 male fisher rats were used in the study. during the first phase of the experiment, the animals were assigned to either group i (n = 10) receiving normal saline or group ii (n = 10) receiving 6 mg/kg adriamycin by tail vein injection 24 hours before surgery. skin grafts with and without fibrin glue were placed over wounds in the backs of th ... | 1987 | 2443938 |
| therapeutic efficacy of antiglioma mesenchymal extracellular matrix 131i-radiolabeled murine monoclonal antibody in a human glioma xenograft model. | the development of mabs, particularly those reactive with primary brain tumors but not with normal brain, provides a potential means of delivering therapeutic agents selectively to human malignant gliomas. mab 81c6, an igg2b immunoglobulin, which defines an epitope of the glioma-associated extracellular matrix protein tenascin, has been shown to bind to human glioma cell lines, glioma xenografts in nude mice, and primary human gliomas, but not to normal adult or fetal brain. to test the therapeu ... | 1988 | 2446747 |
| identification of kallikrein in cultures of adult renal cells. | cortical tubular cultures enriched with distal segments were prepared from the kidney of adult fisher 344 rats bearing thyroid tumors. after two years of cultivation (20 passages) cell monolayers contained immunocytochemically detectable cytokeratin and kallikrein material in their cytoplasm. furthermore cell pellets showed a true renal type of kininogenase activity corresponding to active kallikrein which ranged from 22.3 to 1.5, and total kallikrein from 29.9 to 2.8 pg kinins/min per mg of pro ... | 1987 | 2446783 |
| a sexual dimorphism of mucous cells in the submandibular salivary gland of rat. | submandibular glands of both sexes from one week to six months-old were serially sectioned, and mucous cells showing a strong positive stain to alcian blue ph 2.5, first detected at two weeks, showed a high degree of inter-sectional and inter-individual variability. in males, the percentage of animals having mucous cells increased with sexual maturation and attained 100 per cent at age six months. in females, the maximum (60 per cent) was reached by four weeks and then decreased with sexual matu ... | 1987 | 2451903 |
| generation of thyroglobulin-specific t-cell clones derived from f1 fisher rats. | we established t-cell clones from 'poor-responder' fisher rats specific for thyroglobulin (tg) with a view to examining tg presentation by cloned fisher rat thyroid (frtl) cells. from the screening of 60 t-cell clones, three high-responding tg-specific clones (b21.01, b21.04 and b21.05) were isolated from the lymph nodes of f1 generation (fisher x 'high-responder' buffalo) female rats immunized with murine tg in complete freund's adjuvant (cfa). three t-cell clones expressed a w3/25+, ox-8- phen ... | 1988 | 2454882 |
| [structure of the o-specific polysaccharides and the protective activity of the lipopolysaccharides in pseudomonas aeruginosa of 7 immunotypes (after fisher)]. | the structure of o-specific polysaccharides and the protective activity of lipopolysaccharides (lps) obtained from seven p. aeruginosa immunotypes (according to fisher's classification) have been studied. the structure of o-specific polysaccharides, immunotypes 2, 3, 4, 5 and 6, is identical to that of polysaccharides of serotypes 011; 0(2a), 2c; 01; 010a, 10b; 07a, 7d respectively. no structural analogs of o-polysaccharide characteristic of immunotypes 1 and 7 have been detected among serotypes ... | 1988 | 2459868 |
| seroma prevention using fibrin glue during modified radical neck dissection in a rat model. | seroma is a frequent sequelae of neck dissection involving cervical lymphadenectomy. the incidence is correlated with flap elevation, lymphovascular interruption, and tissue removal. current methods of resolving seroma, such as vacuum drainage, are not risk free. a novel approach to this problem was the use of intraoperative topical fibrin glue. a model producing seromas was developed by modified radical neck dissection on sprague-dawley rats. forty rats underwent this procedure. twenty rats wer ... | 1988 | 2459980 |
| the mechanism of tachykinin-induced bronchoconstriction in the rat. | the mammalian tachykinins substance p (sp) and neurokinin a (nka) are known to be present in sensory airway nerves of animals and humans. we studied the effect of mammalian and nonmammalian tachykinins on the conducting airways of anesthetized, mechanically ventilated fisher 344 rats. dose-dependent increases in lung resistance and decreases in dynamic compliance occurred after the intravenous administration of eledoisin (e), kassinin (k), nka, and sp. e, k, and nka were more potent bronchoconst ... | 1988 | 2461668 |
| enhanced induction of thyroid cell mhc class ii antigen expression in rats highly responsive to thyroglobulin. | initial experiments demonstrated that the degree of autoantibody and proliferative t cell responses to syngeneic rat thyroglobulin differed markedly between buffalo (high responder) and fisher (low responder) rats after classical immunization schedules. while varying immune responsiveness may be due to qualitative and quantitative t and b cell differences, the role of thyroid cell mhc class ii antigens may be pivotal to the onset of autoimmune thyroiditis in such animal models. we, therefore, ex ... | 1989 | 2466637 |
| lithium increases rat striatal beta- and gamma-preprotachykinin messenger rnas. | previous reports indicate that the antimanic drug, lithium, increases substance p-like immunoreactivity (sp-li) in the basal ganglia. the aim of this study was to use lithium as a pharmacological tool to further understand the mechanism of this process. we have used solution hybridization-nuclease protection assays to quantitate the specific preprotachykinin (ppt) mrnas and radioimmunoassays and immunocytochemistry to assess sp-li levels in the striatum of male fisher f-344 rats. a regimen of su ... | 1989 | 2467980 |
| [a study on accumulation of cholesterol in rat prostate]. | an experimental model of prostatic hypertrophy was created by freezing the urethra at the opening of the ventral prostate in rats and administering a cholesterol-rich diet to induce cholesterol accumulation. four-week-old fisher rats were divided into a control group, a cholesterol-rich diet group (by adding 1% cholesterol to the diet), a frozen group, and a frozen and cholesterol-rich diet group, and were reared for 5 months. the animals were killed, and the ventral prostate was removed, weighe ... | 1989 | 2472053 |
| [anthrax toxin dynamics in the body]. | anthrax toxin introduced subcutaneously into white rats fisher-344 caused specific intoxication accompanied by characteristic pathomorphological changes and, when studied by the immunoperoxidase method in histological sections, could be detected in the blood stream (in plasma) and in the cytoplasm of macrophages (in the lungs and the spleen). the content of the preparation introduced into the animals in the blood stream is directly related to its toxicity, being probably indicative of the relati ... | 1989 | 2472713 |
| penetrating keratoplasty in the rat: a model for the study of immunosuppressive treatment of graft rejection. | the present study reports corneal allogeneic transplantation in a rat model. the technique used was full-thickness penetrating keratoplasty. fisher rats were used as donors and lewis rats were used as recipients. the rate of acute rejection obtained in this model with this combination of strains was 100%. it therefore seems to be a very useful model for the study of rejection and for the study of new immunosuppressive treatment. the effect of systemic cyclosporine (csa) treatment on graft reject ... | 1989 | 2474684 |
| traveling waves of in vitro evolving rna. | populations of short self-replicating rna variants have been confined to one side of a reaction-diffusion traveling wave front propagating along thin capillary tubes containing the q beta viral enzyme. the propagation speed is accurately measurable with a magnitude of about 1 micron/sec, and the wave persists for hundreds of generations (of duration less than 1 min). evolution of rna occurs in the wavefront, as established by front velocity changes and gel electrophoresis of samples drawn from a ... | 1989 | 2479013 |
| a cell mutant that exhibits temperature-dependent sensitivity to transformation by various oncogenes. | we previously isolated a fisher rat fibroblast mutant, b812, that has the unique property of temperature-dependent transformation by various oncogenic retroviruses. at the permissive temperature (35 degrees c), this mutant was sensitive to oncogenic transformation and formed foci on a dish at the same frequency as did the parental fibroblast cell line. when kirsten murine sarcoma virus (ki-msv) was applied to the cells, the frequency of focus formation decreased more than 25-fold at the nonpermi ... | 1989 | 2479832 |
| nicotinamide enhances skin flap survival. | the effects of nicotinamide in an abdominal island pedicle skin flap were examined. a 7 x 7 cm island pedicle skin flap ligating the left inferior neurovascular pedicle was created on 50 male sprague dawley rats (250-275 grams) that were divided into five groups. animals received either 0.6 cc of saline or doses of nicotinamide for 16 days (14 days preoperatively and 2 days postoperatively): 25 mg b.i.d., 50 mg b.i.d., 100 mg b.i.d. or 200 mg b.i.d. forty-eight hours postoperatively each animal ... | 1989 | 2482537 |
| use of verbal descriptors, thermal scores and electrical pulp testing as predictors of tooth pain before and after application of benzocaine gels into cavities of teeth with pulpitis. | a double-blind pilot study was conducted on 27 consenting human volunteers who had irreversible pulpitis associated with persistent toothache pain from open carious lesions. formulations tested contained either 0, 10%, or 20% benzocaine and were identified only by a numbered code. before the experiment started, a small amount of a known 5% benzocaine gel was placed for 1 minute on the tongue of each patient to assure a sensation of numbness within the oral cavity. then the test tooth was washed ... | 1989 | 2490060 |
| reovirus induction of mhc class ii antigen in rat thyroid cells. | we have previously demonstrated that cultured rat thyroid cells do not exhibit constitutive expression of major histocompatibility (mhc) class ii antigens. using reovirus types 1 and 3, we infected 1b-6 cells (a cloned derivative of the fisher rat cell line frtl-5), and found a dose-dependent induction of thyroid cell mhc class ii antigen expression as determined by laser flow cytometry and fitc-labelled ox-6 anti-rt1.b. as the number of viral particles/cell used for the infections increased fro ... | 1989 | 2491810 |
| isolation, structure, and immunogenicity of pseudomonas aeruginosa immunotype 4 high-molecular-weight polysaccharide. | a high-molecular-weight, immunogenic form of the lipopolysaccharide o side chain of pseudomonas aeruginosa fisher immunotype 4 (type 4, international antigenic typing system 1, lanyi o:6) was isolated and characterized. analysis by nuclear magnetic resonance spectroscopy confirmed the structural similarity of this high-molecular-weight polysaccharide and the type 4 o side chain. the polysaccharide was immunogenic in rabbits and mice, eliciting opsonophagocytic killing antibodies. immunization wi ... | 1989 | 2492260 |
| high specific activity [samarium-153] edta for imaging of experimental tumor models. | enriched samarium oxide (98.2% 152sm2o3) was irradiated in low neutron flux and high neutron flux reactors to produce 153sm with specific activities of 14.3 gbq and 22.1 tbq mmol-1 sm, respectively, at the time of use. formulation of 153sm as [153sm]edta, with a 1:10 molar ratio of sm:edta, provided a stable radiotracer in vitro and in vivo. high specific activity [153sm]edta showed superior uptake in cell culture (20.8 +/- 0.9% vs. 5.5 +/- 0.1% for 6 and 600 pmol sm per 10(6) cells, respectivel ... | 1989 | 2500500 |
| a reappraisal of the epidemiology of phenotypes of neisseria gonorrhoeae. | we analyzed published data from studies of the epidemiology of gonococcal auxotype/serovar phenotypes in seattle and king county, washington; denver, colorado; and miami and dade county, florida, to determine whether the numbers of new phenotypes were within the ranges of numbers predicted from previous observations in the same populations. for this analysis, we used the model of fisher and good and turing. in all the analyses, the observed number of new species was within the 95% confidence reg ... | 1989 | 2500719 |
| thyrotropin-releasing hormone (trh)-gly conversion to trh in rat ventral prostate is inhibited by castration and aging. | trh levels in rat prostate are very high in the 2-month-old rat and decline at least 90% during the next 2 yr. this decline in prostatic trh levels with aging may be a significant factor in benign prostatic hypertrophy in man and other animals. because prostatic trh correlates positively with serum testosterone and negatively with serum thyroid hormone levels, we have examined the possibility that the age-related decline in prostatic trh is hormonally regulated. trh and a trh precursor peptide, ... | 1989 | 2502375 |
| recognition and binding of template-primers containing defined abasic sites by drosophila dna polymerase alpha holoenzyme. | human dna polymerase alpha holoenzyme follows an ordered sequential terreactant mechanism of substrate recognition and binding (wong, s. w., paborsky, l. r., fisher, p. a., wang, t. s.-f., and korn, d. (1986) j. biol. chem. 261, 7958-7968). we confirmed this mechanism for the dna polymerase alpha holoenzyme purified from drosophila melanogaster embryos and studied the interaction of drosophila pol alpha with synthetic oligonucleotide template-primers containing modified tetrahydrofuran moieties ... | 1989 | 2502545 |
| the structure and serologic distribution of an extracellular neutral polysaccharide from pseudomonas aeruginosa immunotype 3. | previous work has described small molecular weight neutral polysaccharides from isolates of pseudomonas aeruginosa that appear to be associated with the lipopolysaccharide (lps) and distributed across serologic barriers defined by antibody to the o side chain. we have isolated and characterized another of these structures obtained from culture supernatants of an immunotype 3 strain of p. aeruginosa. the isolated neutral polysaccharide has a tetrasaccharide repeat unit: (formula; see text) where ... | 1989 | 2504722 |
| difference in catalepsy response in inbred rats during chronic haloperidol treatment is not predictive of the intensity of behavioral hypersensitivity which subsequently develops. | an animal model was utilized to explore the observed clinical association between a history of significant neuroleptic-induced parkinsonism and an increased incidence for the subsequent development of tardive dyskinesia. catalepsy-sensitive fisher rats and catalepsy-resistant brown norway rats were treated for 14 days with haloperidol at a dose of either 1 mg/kg or 5 mg/kg daily. following a 7-day drug withdrawal period, rats were tested for behavioral hypersensitivity to acute challenge with ap ... | 1989 | 2505286 |
| blockade of conditioned avoidance responding by trazodone, etoperidone, and mcpp. | phenylpiperazines, such as meta-chlorophenylpiperazine (mcpp) a serotonin agonist, have recently been reported to block conditioned avoidance responding (car) in the rat, which is an indication of possible antipsychotic utility. since mcpp is a major metabolite of both antidepressant drugs trazodone (tz) and etoperidone (et), both were examined for activity in blocking car in a single-trial lever press task in fisher 344 rats. both tz and et produced dose-related falls in car with ed50 values (9 ... | 1989 | 2506609 |
| growth hormone (gh) secretion in the pituitary-grafted male rat: in vivo effects of gh-releasing hormone and isoproterenol and in vitro release by individual somatotropes. | although the pituitary-grafted rat is a classic model of chronic prl excess, the presence of somatotropes in grafted pituitary tissue indicates a potential for gh secretion. the current study was designed to investigate gh-releasing hormone (grh)-induced gh secretion and beta-adrenergic inhibition of gh release in animals bearing ectopic pituitary tissue free from hypothalamic control. positive findings with regard to these in vivo experiments led us to an initial determination of gh secretion b ... | 1989 | 2507290 |
| lectin typing of pseudomonas aeruginosa strains of different serogroups, habs and fisher types. | sixteen habs and three fisher types of pseudomonas aeruginosa were typed with lectins of know specificity resulting from their interaction with bacterial cell surface carbohydrates as evidenced by agglutination-inhibition assay with simple carbohydrates. lipopolysaccharides of few strains of pseudomonas are precipitated with different lectins and the results are corroborated by those of agglutination suggesting that pseudomonas aeruginosa can be characterized intraspecifically by lectins. | 1989 | 2508658 |
| isolation and characterization of a human monoclonal antibody that recognizes epitopes shared by pseudomonas aeruginosa immunotype 1, 3, 4, and 6 lipopolysaccharides. | a hybridoma line secreting a human monoclonal antibody (hmab) capable of recognizing fisher immunotype (it) 1, 3, 4, and 6 lipopolysaccharide (lps) in vitro was isolated. peripheral blood lymphocytes (pbl) were obtained from volunteers immunized with an experimental pseudomonas aeruginosa o polysaccharide-toxin a vaccine. pbl-expressing surface antibodies able to bind to p. aeruginosa lps were isolated by adsorption onto lps-coated plastic wells. such pbl were transformed with epstein-barr virus ... | 1989 | 2509371 |
| sarcocystis sp. in mountain goats (oreamnos americanus) in washington: prevalence and search for the definitive host. | during october and november 1986, sarcocystis sp. was detected in 24 of 56 (43%) tongues from hunter-killed mountain goats (oreamnos americanus) in washington (usa). sarcocysts had an unusual sessile polyp-shaped branched wall. mean size of 154 sarcocysts was 71.3 x 37.8 microns (range, 20 to 248 x 10 to 120 microns), and the mean intensity was 2.3 (range, 1 to 28). in an attempt to identify the definitive host, infected tongues were fed to four coyotes (canis latrans), eight domestic dogs, four ... | 1989 | 2509738 |
| [specific intoxication in anthrax infection]. | the pathomorphological picture of experimental b. anthracis infection in white rats (strain fisher-344) essentially corresponds to experimental anthracic intoxication with very moderately pronounced morphological manifestation of b. anthracis invasion. this indicates that specific anthracic intoxication is an essential component of the pathological process in b. anthracis infection. | 1989 | 2511710 |
| effects of diethylstilboestrol on testicular function and luteinizing hormone receptors. | adult male fisher-344 rats were implanted with des-filled or empty silastic capsules. after 14 weeks, capsules were removed and a second group of rats received des capsules. seven weeks later, all the rats were sacrificed. des treatment decreased body, testes and seminal vesicle weights, and removal of the capsules partially restored the weight of these organs. the concentration of testicular lh receptors was increased by des treatment. circulating prl levels were increased and gonadotropin leve ... | 1989 | 2515561 |
| [results of cross-species transplantation of substantia nigra to the lateral ventricle of the brain of rats with experimentally-induced parkinson disease]. | the method of compensation of dopamine (da) deficit in the striatum by living, da producing cells implanted into the brain, creates new hopes for patients with parkinson's disease. in view of many ethical and legal problems, connected in some countries with obtaining human fetal tissue for transplantation, cross-species transplants would be an attractive alternative. the subject of our investigation was the problem of survival and functioning of embryonal substantia nigra (sn) neurons, taken fro ... | 1989 | 2518574 |
| the calmodulin binding domain of the plasma membrane ca2+ pump interacts both with calmodulin and with another part of the pump. | synthetic peptides corresponding to the calmodulin-binding domain of the human erythrocyte ca2+ pump were prepared representing residues 2-29 (c28w), 2-21 (c20w), 2-16 (c15w), and 16-29 (c14) of the sequence (james, p., maeda, m., fisher, r., verma, a. k., krebs, j., penniston, j. t., and carafoli, e. (1988) j. biol. chem. 263, 2905-2910). peptides c28w, c20w, and c15w bound to calmodulin with an apparent 1:1 stoichiometry in the presence of ca2+ and inhibited the activation of the ca2+ pump by ... | 1989 | 2526124 |
| interleukins production and responsiveness in fisher rats with adjuvant-induced disease: role of suppressor cells. | splenic macrophages from aid fisher rats produce normal levels of il-1, in contrast to il-2 which is significantly lower than normal. a state of hyporesponsiveness of spleen cells to con-a and pha occurs with similar kinetics to and is correlated with the development of an articular arthritis; il-1 addition to aid spleen cell cultures does restore to normal their deficient response, in contrast to addition of il-2 or ril-2. the spleen cells from normal or aid rats are equally sensitive to the in ... | 1989 | 2526223 |
| complementation of enteric adenovirus type 40 for lytic growth in tissue culture by e1b 55k function of adenovirus types 5 and 12. | the enteric adenovirus type 40 strain dugan (ad40) cannot be passaged in hela cells, but will grow in 293 cells, which express ad5 e1 functions. to determine the reason for this limited host range, kb cell lines expressing ad2 e1a, e1b, or e1a + e1b (l. e. babiss, c. s. h. young, p. b. fisher, and h. s. ginsberg, 1983, j. virol. 46, 454-465) have been tested for their ability to support ad40 replication. only cell lines which supply e1b functions, but not those expressing e1a alone, are permissi ... | 1989 | 2527440 |
| functional analysis of lymphocytes subpopulations in experimental cocaine abuse. i. dose-dependent activation of lymphocyte subsets. | the potential role of substance abuse, especially cocaine and alcohol as co-factor in hiv infection and in the development and expression of aids has been suggested, but the possible biological role of substance abuse in the development of aids is not known. in order to better understand immune system function in chronic cocaine abuse, we have assessed primary b cell responses to helper t-cell independent (ti) and dependent (td) antigens in inbred fisher male rats injected with 1.25-5 mg cocaine ... | 1989 | 2528433 |
| effect of cyproterone acetate in comparison to flutamide on the ventral prostate of adult male castrated copenhagen-fisher rats and on dunning r-3327 h tumors. | in this investigation the effect of cpa was tested in comparison to fl after the procedure of double blinding on the ventral prostate of 70 adult male castrated copenhagen-fisher rats and on the dunning r-3327 h tumor. total androgen blockade by castration plus cpa or by castration plus fl induced significant decrease in prostate weight compared to the androgen deprivation by castration alone. no significant difference between cpa and fl was observed. furthermore it was impossible to exaggerate ... | 1989 | 2530921 |
| normal brain tissue response to photodynamic therapy: histology, vascular permeability and specific gravity. | the response of photodynamic therapy on normal brain was investigated in 140 fisher rats. the rats were injected i.p. with photofrin ii (12.5 mg/kg) and 48 h later the dural area over the frontal cortex was photoactivated with red light (630 +/- 1 nm) from an argon dye laser. treatment was performed with optical energy densities of 140 and 70 j/cm2. histopathology, vascular permeability and specific gravity measurements were conducted on different populations of rats at 4 h, 24 h, 72 h and 1 wee ... | 1989 | 2533693 |
| immunological functions in food-restricted rats: enhanced expression of high-affinity interleukin-2 receptors on splenic t cells. | several immunological functions of b and t cells including il-2 receptor expression on t cells were measured in 12-month-old fisher-344 male rats maintained from 6 weeks of age on an ad libitum (al) or a 40% food-restricted (fr) diet. direct anti-srbc plaque-forming cell (pfc) assays revealed a higher response in fr rats than in al rats when splenocytes were cultured with or without recombinant interleukin-2 (ril-2). b cell functions were studied by using nylon wool-purified splenic b cells stim ... | 1989 | 2534390 |
| triethyllead-induced peroxidative damage in various regions of the rat brain. | adult male fisher 344 rats (8-10 wk old) were dosed ip with 1.75 mg/kg body weight of triethyllead chloride (tel) for 5 consecutive days. rats were sacrificed 1, 7, or 21 d after the last injection. the rate of lipid peroxidation was significantly elevated in frontal cortex at all three time points assayed (1, 7, or 21 d). however, hippocampal and cerebellar membranes showed no changes in peroxidative capacity at these time points. in order to determine whether cortical membrane damage was refle ... | 1989 | 2537905 |
| vip receptors and content after bowel transplantation. | advances in immunosuppressive therapy have renewed interest in small bowel transplantation. little is known, however, about the functional capacity of transplanted intestine. to clarify the potential for normal function, we investigated whether elements of the enteric nervous system are preserved after denervation in our rat model of intestinal transplantation. we investigated whether vip, a major peptide neurotransmitter of the enteric nervous system, and its receptors are preserved in the bowe ... | 1989 | 2541280 |
| diagnostic usefulness of mycobacterial skin test antigens in childhood lymphadenitis. | nontuberculous mycobacterial (ntm) infections are a frequent cause of chronic lymphadenitis in children. previous studies of ntm antigen skin testing were inconclusive as a result of problems with study design and antigen formulation. the present study was undertaken with the centers for disease control to determine whether newly formulated ntm skin test antigens applied in a double blind manner with a standard purified protein derivative could accurately distinguish ntm infections from those ca ... | 1989 | 2541397 |
| fbl-reactive cd8+ cytotoxic and cd4+ helper t lymphocytes recognize distinct friend murine leukemia virus-encoded antigens. | immunization of c57bl/6 (b6) mice with fbl, a friend murine leukemia virus (f-mulv), induces both tumor-specific cytolytic cd8+ (ctl) and lymphokine-producing cd4+ th that are effective in adoptive therapy of b6 mice bearing disseminated fbl leukemia. the current study evaluated the f-mulv antigenic determinants expressed on fbl that are recognized by fbl-reactive cd8+ and cd4+ t cells. to identify the specificity of the fbl-reactive cd8+ ctl, fisher rat embryo fibroblast (fre) cells transfected ... | 1989 | 2562982 |
| [ergonomic analysis of the work sites and living conditions of the crew on board the b-418/111 t/h "bonito" ppd and ur "gryf" fishing trawlers]. | the paper is an ergonomic study of the sanitary and living conditions at the working place of fishers on long-distance fishing vessels, such as the trawler b-418/111 t/h "bonito". the research was performed during the trip in the region of the falkland islands (malvinas). prophylactic examinations were carried out during 162 days on 77 members of the crew and the hygienic-sanitary conditions on the vessel were analysed. the ergonomic research included all the working places of the members of the ... | 1989 | 2571193 |
| activation of a ribosomal s6 protein kinase in rapidly emerging diethylnitrosamine-induced gamma-glutamyltranspeptidase-positive hyperplastic liver lesions of the rat. | the gamma-glutamyl transpeptidase (ggt)-positive hyperplastic liver lesions which developed in the fisher 344 rat 7 and 60 days following a single carcinogenic dose of diethylnitrosamine (dena, 200 mg/kg body weight), short-term dietary exposure to 0.02% 2-acetylaminofluorene (aaf) to suppress the growth of normal hepatocytes, and partial hepatectomy to actuate rapid growth of dena altered hepatocytes not suppressed by aff, showed an increased activity of a kinase which specifically phosphorylat ... | 1989 | 2575815 |
| distribution of the c-myc oncoprotein in normal and neoplastic tissues of the rat colon. | a rat model of 5-azoxymethane induced colon cancer was studied in order to correlate histopathological changes and the differential distribution of the c-myc protein. weanling fisher 344 rats were injected with three, one week apart, subcutaneous injections of 5-azoxymethane (aom) (15 mg kg-1) and the animals were divided into low and high fat diet groups. nine colon tumors, of varying degrees of malignancy, that developed in the aom-treated rats, and sections of normal colonic mucosa were exami ... | 1988 | 2577872 |
| pausing of rna polymerase during in vitro transcription through the ilvb and ilvgeda attenuator regions of escherichia coli k12. | synchronized single-round transcriptions, in vitro, from templates encoding the leader rna of the ilvb and ilvgeda operons result in the accumulation of a transcript consistent with rna polymerase pausing after the 1:2 stem that could form in each of the leader rnas. addition of l-factor or guanosine 5'-diphosphate,3-diphosphate extended the pause half-life obtained with the ilvb template; addition of l-factor and guanosine 5'-diphosphate,3'-diphosphate together had an additive effect on the pau ... | 1985 | 2578464 |
| some simple methods for linkage analysis. | a method of analysis of suitable many-locus linkage data used by fisher in 1922 is re-examined in detail, and it is pointed out that it could be used also in human genetics as a simple method for preliminary examination of data. | 1989 | 2596834 |
| alteration of tissue magnesium levels in rats by dietary vitamin b6 supplementation. | tissues from female fisher rats fed varying levels of magnesium (100 ppm or 700 ppm) and pyridoxine hydrochloride (pn.hcl) (7, 35, or 1500 mg/kg diet for six weeks) were dry ashed and analyzed for magnesium using atomic absorption spectroscopy. in tissues from rats consuming magnesium diets, tissue magnesium levels increased as levels of dietary pn.hcl increased. increases in plasma, liver, kidney, and brain magnesium levels were statistically significant (p less than 0.05). with the exception o ... | 1989 | 2599796 |
| cholesterol esterification plays a major role in determining low-density-lipoprotein receptor activity in primary monolayer cultures of rat hepatocytes. | 1. we have previously shown that the capacity for specific binding of human 125i-labelled low-density lipoprotein (ldl) to rat hepatocytes increases with time in culture [salter, bugaut, saxton, fisher & brindley (1987) biochem. j. 247, 79-84]. 2. in the present study we show that this up-regulation is accompanied by a rise in the cholesterol ester content of the cells. 3. inhibition of cholesterol esterification with the drug 58-035 (sandoz) significantly decreases the time-dependent 'up-regula ... | 1989 | 2604696 |
| changes of blood flow in the adenohypophysis of normal and estrogen pretreated fisher rats by tamoxifen. | blood flow was measured in the adenohypophysis and in the cerebral cortex of female f344 rats over a period of 90 min using the hydrogen clearance method. tamoxifen, 1 mg/kg, administered iv reduced the blood flow in the adenohypophysis by 35%, whereas cerebral blood flow and arterial pressure remained unchanged. seven days sc treatment with tamoxifen (1 mg/kg daily) had no demonstrable effect on blood flow. anterior pituitary hyperplasia was induced in 15 rats with diethylstilbestrol containing ... | 1989 | 2609903 |
| comparative cariogenicity and dental plaque-forming ability in gnotobiotic rats of four species of mutans streptococci. | the four species, each represented by two strains, all produced high levels of fissure caries in the upper and lower jaws of gnotobiotic fisher rats. streptococcus rattus strains and streptococcus mutans nctc 10832 gave low levels of approximal caries, whereas streptococcus cricetus, streptococcus sobrinus and strep. mutans nctc 10449 gave high levels. the only strains to produce buccal surface lesions were strep. sobrinus and strep. cricetus. all strains were able to form dense plaque in the ce ... | 1989 | 2610617 |
| iodination of newly synthesized thyroglobulin by frtl-5 cells is selective and thyrotropin dependent. | this study shows that the fisher rat thyroidal cell line (frtl-5) can iodinate newly synthesized thyroglobulin. iodinated thyroglobulin was found intra- and extracellularly. both the synthesis of thyroglobulin and its subsequent iodination were found to be thyrotropin (tsh) dependent, with optimal activity at 10-100 microu tsh/ml. thyroglobulin was the only protein in the culture medium, that was iodinated with high specificity and in a tsh-dependent fashion. albumin, which was abundantly presen ... | 1989 | 2612732 |
| anatomical and functional effects of estrogen-induced prolactinomas on the rat hypothalamus. | although estrogen-induced prolactinomas have been widely studied, little attention has been accorded to local pressure effects of the tumor on the hypothalamus and portal vasculature. to portray the magnitude of this phenomenon, four groups of 12-13-week-old female fisher 344 rats were studied. group 1 was an intact control receiving a subcutaneously (sc) placed placebo pellet; group 2 was an ovariectomized control with a sc placed placebo pellet; group 3 was ovariectomized with a 10 mg sc place ... | 1989 | 2614531 |
| the effect of marijuana smoke exposure on murine sarcoma 180 survival in fisher rats. | fisher rats were treated for 28 or 60 days to multiple exposures to the smoke of marijuana or marijuana placebo cigarettes. primary, secondary and in some instances tertiary tumor implants were performed. murine sarcoma 180 tumor cells (7.5 x 10(7)) were implanted subcutaneously on day 1, 14 and 28 following initiation of smoke exposure (28 day studies) or on day 1, 14 after cessation of smoke exposure (60 day studies). tumor areas were measured on alternate days beginning on the second or third ... | 1989 | 2621317 |
| phorbol esters and forskolin infused into midbrain central gray facilitate lordosis. | several peptides, when infused into the mcg, facilitate lordosis in estrogen-primed female rats. since these peptides can act through camp and/or protein kinase c, and these second messenger systems have been implicated in neuromodulation, this study examined if pharmacological agents which stimulate these systems would facilitate lordosis. ovariectomized female fisher rats were given bilateral cranial cannulae targeted to the mcg, or cortex dorsal to mcg, and allowed at least a week to recover. ... | 1989 | 2623023 |
| evaluation of cortisone-heparin and cortisone-maltose tetrapalmitate therapies against rodent tumors. i. biological studies. | the antitumor activity of either cortisone-heparin or cortisone-maltose tetrapalmitate combination or both was tested against two animal tumor models. the first model was orthotopically implanted bladder tumor established in syngeneic fisher 344 rats. shrinkage and growth arrest of the tumors were induced by cortisone and amplified by its combination with either heparin or maltose tetrapalmitate (mtp). the second model was trocar implanted c3hba mammary tumor piece s.c. in syngeneic lps and mtp ... | 1989 | 2627134 |
| evaluation of cortisone-heparin and cortisone-maltose tetrapalmitate therapies against rodent tumors. ii. pathological studies. | pathological studies were undertaken in three tumor-host models which were subjected to cortisone based treatments. the first model was fisher 344 rats with established orthotopically implanted syngeneic bladder tumor. cortisone-herapin and cortisone-maltose tetrapalmitate (mtp) treatments induced focal areas of tumor necrosis and necrobiosis, whereas cortisone alone caused necrobiosis. the second model was c3hba mammary tumor fragments implanted subcutaneously in syngeneic mtp responder c3h/hen ... | 1989 | 2627135 |
| natural killer cell activity in rats infused with intralipid. | the effect of intralipid on the natural killer (nk) cell activity of healthy male fisher 344 adult rats was investigated. they were cannulated via the right jugular vein and continuously infused for five days with: normal saline plus heparin, 5% intralipid plus heparin, or 10% intralipid plus heparin. control groups comprised of cannulated rats receiving no infusion and rats undergoing no operative procedures. following the five-day infusion, rats were exsanguinated under ether anesthesia and mo ... | 1989 | 2628583 |
| endogenous glutamate release from frontal cortex of adult and aged rats. | glutamate (glu) is a major excitatory neurotransmitter in the frontal cortex. alterations in glu neurotransmission are present in a number of neurodegenerative diseases, however, little is known about the normal aging process of glu utilizing neurons. glu release, uptake and content were examined in the frontal cortex of adult (6 months old) and aged (24 months old) male, fisher 344 rats. these markers were used to assess the functional integrity of intrinsic and extrinsic glu utilizing pathways ... | 1989 | 2628777 |