| substructure within salmonella enterica subsp. enterica isolates from australian wildlife. | multilocus sequence typing of 56 salmonella enterica subsp. enterica strains isolated from australian wildlife hosts was performed. the results of population assignment algorithms revealed that the 56 strains could be subdivided into two distinct clades. strains belonging to the two clades were further distinguished phenotypically, genotypically, and with respect to host distribution. | 2011 | 21378038 |
| [investigation of potential risk factors for salmonella enterica subsp enterica serotype napoli: a nested case-control study in lombardia region]. | enter-net surveillance system has detected the re-emergence of salmonella enterica subsp enterica serotype napoli (s. napoli) since 2002. preliminary data show that food vehicle could not be the only one implicated in the transmission of this serotype. a nested case-control study has been conducted using data form a prospective cohort: for each salmonellosis notification in lombardia from may 2004 to december 2005 an ad hoc epidemiological investigation has been performed. cases have been define ... | 2010 | 21425643 |
| antimicrobial resistance, virulence-associated genes, and pulsed-field gel electrophoresis profiles of salmonella enterica subsp. enterica serovar typhimurium isolated from piglets with diarrhea in korea. | salmonella enterica subsp. enterica serovar typhimurium was isolated from diarrheic piglets in 2 periods, 2000-2001 (n = 25) and 2005-2006 (n = 17). to compare the characteristics of the isolates collected during the 2 periods, all isolates were tested for antimicrobial resistance, the presence of virulence genes, and pulsed-field gel electrophoresis (pfge) patterns. all 42 isolates were resistant to at least 1 of the 20 antimicrobials tested, and 39 (93%) were resistant to 2 or more antimicrobi ... | 2011 | 21461195 |
| monoclonal antibodies to lipopolysaccharide antigens of salmonella enterica serotype typhimurium dt104. | salmonella enterica subsp. enterica serotype typhimurium is one of the major causative agents of human gastroenteritis. here we raised a panel of 45 monoclonal antibodies (mabs) against ser. typhimurium dt104 by immunizing mice with formalin-killed bacteria and demonstrated that all the mabs recognized the bacterial lipopolysaccharide (lps) antigen. these mabs were specific for group o:4 salmonella with very little or no cross-reactivity with other closely related bacteria and were able to bind ... | 2011 | 21466285 |
| culling decisions of dairy farmers during a 3-year salmonella control study. | salmonella enterica subsp. enterica-serotypes lead to periodically increased morbidity and mortality in cattle herds. the bacteria can also lead to serious infections in humans. consequently, denmark has started a surveillance and control programme in 2002. the programme focuses on salmonella dublin which is the most prevalent and most persistent serotype in the danish cattle population. a field study in 10 dairy herds with persistent salmonella infections was carried out over three years to gai ... | 2011 | 21481960 |
| russian tortoises (agrionemys horsfieldi) as a potential reservoir for salmonella spp. | a total of 80 russian tortoises brought in poland were examined for presence of salmonella. salmonella was detected in 15 out of all the animals tested (18.75%). of the total of 56 strains, 30 (53.57%) belonged to salmonella enterica subsp. enterica (i) and 26 to salmonella enterica subsp. salamae (ii). the predominant serotype within subspecies i was s. newport, which is one of the most serotypes causing salmonellosis in humans and warm-blooded animals. in vitro determination of the susceptibil ... | 2011 | 21486674 |
| ultra-fast and sensitive detection of non-typhoidal salmonella using microwave-accelerated metal-enhanced fluorescence ("mamef"). | certain serovars of salmonella enterica subsp. enterica cause invasive disease (e.g., enteric fever, bacteremia, septicemia, meningitis, etc.) in humans and constitute a global public health problem. a rapid, sensitive diagnostic test is needed to allow prompt initiation of therapy in individual patients and for measuring disease burden at the population level. an innovative and promising new rapid diagnostic technique is microwave-accelerated metal-enhanced fluorescence (mamef). we have adapted ... | 2011 | 21494634 |
| equivalence of quality control strains of microorganisms used in the compendial microbiological tests: are national culture collection strains identical? | the pharmacopoeias list a number of microorganisms to be used in the compendial microbiological tests for confirming the growth-promoting, indicative, and inhibitory properties of the media and demonstrating the suitability of the test for a specific test article. major national culture collections are specified as the sources for these test strains based on their history of deposition and maintenance and use in the compendial tests. using these microorganisms, it has long been assumed that thes ... | 2010 | 21502014 |
| national outbreak of salmonella enteritidis phage type 14b in england, september to december 2009: case-control study. | we conducted an unmatched retrospective case–control study to investigate an upsurge of non-travel-related sporadic cases of infection with salmonella enterica subsp. enterica serotype enteritidis phage type 14b with antimicrobial resistance to nalidixic acid and partial resistance to ciprofloxacin (s. enteritidis pt 14b nxcp(l)) that was reported in england from 1 september to 31 december 2009. we analysed data from 63 cases and 108 controls to determine whether cases had the same sources of in ... | 2011 | 21507321 |
| rapid screening of epidemiologically important salmonella enterica subsp. enterica serovars using whole-cell maldi-tof mass spectrometry. | currently, 2,610 different salmonella serovars are described according to the white-kauffmann-le minor scheme. they are routinely differentiated by serotyping, which is based on the antigenic variability at lipopolysaccharide moieties (o antigens), flagellar proteins (h1 and h2 antigens), and capsular polysaccharides (vi antigens). the aim of this study was to evaluate the potential of maldi-tof mass spectrometry for rapid screening and identification of epidemiologically important salmonella en ... | 2011 | 21515723 |
| inappropriate use of d-values for determining biocidal activity of various antimicrobials. | the objective of this study was to investigate the application of established d-value calculations to survival curves for various bacteria using the following antimicrobials: acidified sodium chlorite, triclosan, octanoic acid, and sodium hydroxide. d-values can be calculated in 3 ways, a linear regression, an endpoint calculation, or an average of multiple endpoint calculations. the assumption made in calculating a d-value is that the rate of kill follows 1st-order kinetics under specified trea ... | 2011 | 21535698 |
| dry-resistance of salmonella enterica subsp. enterica serovar enteritidis is regulated by both sep22, a novel pathogenicity-related factor of salmonella, and nutrients. | environmental isolates of salmonella enterica serover enteritidis (s. enteritidis) clones were grown to the logarithmic phase, washed and re-suspended in saline or luria-bertani (lb) medium, and then 10-µl aliquots of the suspensions were dried overnight at room temperature. the dried bacteria were mixed with 1 ml of ice-cold pbs, suspended and examined for colony-forming activity. all of the pathogenic clones with high levels of sep22, identical to salmonella dps, maintained good viability if s ... | 2009 | 21566364 |
| onset of diarrhea and pyrexia and time to detection of salmonella enterica subsp enterica in feces in experimental studies of cattle, horses, goats, and sheep after infection per os. | to determine time to first detection of salmonella organisms in feces of animals after experimental infection po and times to onset of diarrhea and pyrexia to evaluate a common method for identifying nosocomial infections on the basis of time of admission and onset of clinical signs (ie, the 3-day criterion). | 2011 | 21568780 |
| application of multiple locus variable number of tandem repeat analysis (mlva), phage typing and antimicrobial susceptibility testing to subtype salmonella enterica serovar typhimurium isolated from pig farms, pork slaughterhouses and meat producing plants in ireland. | salmonella enterica subsp. enterica serovar typhimurium is a common zoonotic pathogen encountered in irish pigs and the pork industry and its characterisation using highly discriminatory typing methods is necessary for epidemiological studies, outbreak investigation and control. multiple locus variable number of tandem repeat analysis (mlva), phage typing and antimicrobial susceptibility testing were applied to characterise 301 s. typhimurium isolates of porcine origin isolated from farms, slaug ... | 2011 | 21569956 |
| subtyping salmonella serovar enteritidis isolates from different sources using sequence typing based on virulence genes and crisprs. | salmonella enterica subsp. enterica serovar enteritidis is a major cause of foodborne salmonellosis in the united states. two major food vehicles for ser. enteritidis are contaminated eggs and chicken meat. improved subtyping methods are needed to accurately track specific strains of ser. enteritidis related to human salmonellosis throughout the chicken and egg food system. a sequence typing scheme based on virulence genes (fimh and ssel) and crisprs (clustered regularly interspaced short palind ... | 2011 | 21571881 |
| prevalence and counts of salmonella spp. in minimally processed vegetables in são paulo, brazil. | minimally processed vegetables (mpv) may be important vehicles of salmonella spp. and cause disease. this study aimed at detecting and enumerating salmonella spp. in mpv marketed in the city of são paulo, brazil. a total of 512 samples of mpv packages collected in retail stores were tested for salmonella spp. and total coliforms and escherichia coli as indication of the hygienic status. salmonella spp. was detected in four samples, two using the detection method and two using the counting method ... | 2011 | 21645825 |
| multi-locus sequence typing of salmonella enterica subsp. enterica serovar enteritidis strains in japan between 1973 and 2004. | salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. this study determined s. enteritidis lineages before and after this pandemic event in japan using multilocus sequence typing (mlst). thirty s. enteritidis strains were collected in japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovi ... | 2011 | 21672260 |
| effects of two simulated oxytetracycline dosing regimens on horizontal transfer of antimicrobial resistance plasmids in an in vitro pharmacodynamic model. | to evaluate the impact of oxytetracycline exposure on horizontal transfer of an antimicrobial resistance plasmid. | 2011 | 21728847 |
| a multiplex real-time pcr assay targeting virulence and resistance genes in salmonella enterica serotype typhimurium. | abstract: | 2011 | 21707966 |
| specific detection of campylobacter jejuni using the bacteriophage nctc 12673 receptor binding protein as a probe. | campylobacter jejuni is found in the intestines of poultry, cattle, swine, wild birds and pet animals and is the major cause of foodborne gastroenteritis in developed countries. we report the use of the receptor binding protein (rbp) of campylobacter bacteriophage nctc 12673 for the specific capture of campylobacter jejuni bacteria using rbp-derivatized capturing surfaces. the gp48 rbp was expressed as a glutathione s-transferase-gp48 (gst-gp48) fusion protein and immobilized onto surface plasmo ... | 2011 | 21955997 |
| comparison of two possible routes of pathogen contamination of spinach leaves in a hydroponic cultivation system. | the route of pathogen contamination (from roots versus from leaves) of spinach leaves was investigated with a hydroponic cultivation system. three major bacterial pathogens, escherichia coli o157:h7, salmonella, and listeria monocytogenes, were inoculated into the hydroponic solution, in which the spinach was grown to give concentrations of 10⁶ and 10³ cfu/ml. in parallel, the pathogens were inoculated onto the growing leaf surface by pipetting, to give concentrations of 10⁶ and 10³ cfu per leaf ... | 2011 | 21902924 |
| Detecting non-typhoid Salmonella in humans by ELISAs: a literature review. | Non-typhoid salmonellosis is one of the most common causes of foodborne illness throughout the world. Serological methods for the diagnosis of Salmonella infections vary widely and the most commonly used test is limited by high running costs as well as low sensitivity and specificity. Fast and reliable immunoassays which detect subunit antigens for Salmonella enterica subsp. enterica serovar Typhi are commercially available but at present there is no international consensus on similar tests for ... | 2012 | 22034162 |
| Identification of environmental reservoirs of nontyphoidal salmonellosis: aptamer-assisted bioconcentration and subsequent detection of salmonella typhimurium by quantitative polymerase chain reaction. | In this study, identification of environmental reservoirs of Salmonella enterica subsp. enterica serovar Typhimurium (abbreviated as Salmonella Typhimurium) in sediments, water, and aquatic flora collected from the Ganges River (Ganges riverine material) was carried out by adopting a two-step strategy. Step 1 comprised a selective serovar-specific capture of Salmonella Typhimurium from potential reservoirs. Step 2 involved culture-free detection of selectively captured Salmonella Typhi ... | 2011 | 21875107 |
| pulsed-field gel electrophoresis supports the presence of host-adapted salmonella enterica subsp. enterica serovar typhimurium strains in the british garden bird population. | salmonellosis is a frequently diagnosed infectious disease of passerine birds in garden habitats within great britain with potential implications for human and domestic animal health. postmortem examinations were performed on 1,477 garden bird carcasses of circa 50 species from england and wales, 1999 to 2007 inclusive. salmonellosis was confirmed in 263 adult birds of 10 passerine species in this 11-year longitudinal study. a subset of 124 fully biotyped salmonella enterica subsp. enterica sero ... | 2011 | 21948838 |
| tn1548-associated arma is co-located with qnrb2, aac(6')-ib-cr and blactx-m-3 on an incfii plasmid in a salmonella enterica subsp. enterica serovar paratyphi b strain isolated from chickens in china. | | 2012 | 21965429 |
| Phenotypic and molecular characterization of human salmonella enterica serovar 4,[5],12:i:- isolates in Slovakia. | Forty-three epidemiologically unrelated emerging Salmonella enterica subsp. enterica serovar 4,[5],12:i:- strains isolated during the period 2009-2010 in Slovakia were characterized by phenotypic and genotypic methods. Thirty-one isolates (72.1%) expressed resistance to ampicillin, streptomycin, sulfizoxazole, and tetracycline [R-type ASSuT]. The majority of the strains belonged to both definitive phage types DT193 (30.2%) and U311 (27.9%). Other phage types identified were U302 (6.9%), DT18 (4. ... | 2011 | 21909783 |
| methodologies for salmonella enterica subsp. enterica subtyping: gold standards and alternatives. | for more than 80 years, subtyping of salmonella enterica has been routinely performed by serotyping, a method in which surface antigens are identified based on agglutination reactions with specific antibodies. the serotyping scheme, which is continuously updated as new serovars are discovered, has generated over time a data set of the utmost significance, allowing long-term epidemiological surveillance of salmonella in the food chain and in public health control. conceptually, serotyping provide ... | 2011 | 21856826 |
| Extended-spectrum ß-lactamases in German isolates belonging to the emerging monophasic Salmonella enterica subsp. enterica serovar Typhimurium 4,[5],12:i:- European clone. | | 2011 | 22058374 |
| a whole-genome single nucleotide polymorphism-based approach to trace and identify outbreaks linked to a common salmonella enterica subsp. enterica serovar montevideo pulsed-field gel electrophoresis type. | in this study, we report a whole-genome single nucleotide polymorphism (snp)-based evolutionary approach to study the epidemiology of a multistate outbreak of salmonella enterica subsp. enterica serovar montevideo. this outbreak included 272 cases that occurred in 44 states between july 2009 and april 2010. a case-control study linked the consumption of salami made with contaminated black and red pepper to the outbreak. we sequenced, on the solid system, 47 isolates with xbai pfge pattern jixx01 ... | 2011 | 22003026 |
| Prevalence of Salmonella enterica and the hygienic indicator Escherichia coli in raw meat at markets in Ouagadougou, Burkina Faso. | This study investigated the hygienic status and prevalence of Salmonella and Escherichia coli in retail meat sold at open markets in Ouagadougou, Burkina Faso. A total of 150 samples of beef meat (n = 45), beef intestine (n = 45), mutton (n = 30), and chicken (n = 30) were collected from four local markets for investigation. The prevalence of Salmonella enterica subsp. enterica was 9.3%, and six serotypes, all previously unreported in Burkina Faso, were identified: Derby, Tilene, Hato, Bredeney, ... | 2011 | 21902926 |
| Genome sequencing reveals diversification of virulence factor content and possible host adaptation in distinct subpopulations of Salmonella enterica. | Divergence of bacterial populations into distinct subpopulations is often the result of ecological isolation. While some studies have suggested the existence of Salmonella enterica subsp. enterica subclades, evidence for these subdivisions has been ambiguous. Here we used a comparative genomics approach to define the population structure of Salmonella enterica subsp. enterica, and identify clade-specific genes that may be the result of ecological specialization. | 2011 | 21859443 |
| antimicrobial and safety properties of lactobacilli isolated from two cameroonian traditional fermented foods. | twenty-one lactobacillus isolates from "sha'a" (a maize - based fermented beverage) and "kossam" (traditionally fermented cow milk) were selected in accordance with their antagonistic activities and tested for their bacteriocinogenic potential as well as safety properties. these isolates were preliminarily identified as lactobacillus plantarum (62%), lactobacillus rhamnosus (24%), lactobacillus fermentum (10%) and lactobacillus coprophilus (4%) based on phenotypic characteristics and rep-pcr gen ... | 2011 | 22396914 |
| the combination of crispr-mvlst and pfge provides increased discriminatory power for differentiating human clinical isolates of salmonella enterica subsp. enterica serovar enteritidis. | salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) is a major cause of foodborne salmonellosis. rapid, efficient and accurate methods for identification are required to track specific strains of s. enteritidis during outbreaks of human salmonellosis. by exploiting the hypervariable nature of virulence genes and clustered regularly interspaced short palindromic repeats (crisprs), we previously developed a powerful sequence-based subtyping approach, designated crispr-mvlst. t ... | 2012 | 23498194 |
| specific discrimination of three pathogenic salmonella enterica subsp. enterica serotypes by carb-based oligonucleotide microarray. | it is important to rapidly and selectively detect and analyze pathogenic salmonella enterica subsp. enterica in contaminated food to reduce the morbidity and mortality of salmonella infection and to guarantee food safety. in the present work, we developed an oligonucleotide microarray containing duplicate specific capture probes based on the carb gene, which encodes the carbamoyl phosphate synthetase large subunit, as a competent biomarker evaluated by genetic analysis to selectively and efficie ... | 2014 | 24185846 |
| comparative genomics of closely related salmonella enterica serovar typhi strains reveals genome dynamics and the acquisition of novel pathogenic elements. | typhoid fever is an infectious disease of global importance that is caused by salmonella enterica subsp. enterica serovar typhi (s. typhi). this disease causes an estimated 200,000 deaths per year and remains a serious global health threat. s. typhi is strictly a human pathogen, and some recovered individuals become long-term carriers who continue to shed the bacteria in their faeces, thus becoming main reservoirs of infection. | 2014 | 25412680 |
| genomic characterization of salmonella cerro st367, an emerging salmonella subtype in cattle in the united states. | within the last decade, salmonella enterica subsp. enterica serovar cerro (s. cerro) has become one of the most common serovars isolated from cattle and dairy farm environments in the northeastern us. the fact that this serovar is commonly isolated from subclinically infected cattle and is rarely associated with human disease, despite its frequent isolation from cattle, has led to the hypothesis that this emerging serovar may be characterized by reduced virulence. we applied comparative and popu ... | 2014 | 24898914 |
| salmonella utilizes d-glucosaminate via a mannose family phosphotransferase system permease and associated enzymes. | salmonella enterica is a globally significant bacterial food-borne pathogen that utilizes a variety of carbon sources. we report here that salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) uses d-glucosaminate (2-amino-2-deoxy-d-gluconic acid) as a carbon and nitrogen source via a previously uncharacterized mannose family phosphotransferase system (pts) permease, and we designate the genes encoding the permease dgaabcd (d-glucosaminate pts permease components eiia, eiib, e ... | 2013 | 23836865 |
| loss of culturability of salmonella enterica subsp. enterica serovar typhimurium upon cell-cell contact with human fecal bacteria. | loss of culturability of salmonella enterica subsp. enterica serovar typhimurium has been observed in mixed cultures with anaerobic fecal bacteria under conditions that allow local interaction between cells, such as cell contact. a reduction of a population of culturable s. typhimurium on the order of ∼10(4) to 10(5) cfu/ml was observed in batch anaerobic mixed cultures with fecal samples from different human donors. culturability was not affected either in supernatants collected at several time ... | 2013 | 23503308 |
| effects of norspermidine and spermidine on biofilm formation by potentially pathogenic escherichia coli and salmonella enterica wild-type strains. | polyamines are present in all living cells. in bacteria, polyamines are involved in a variety of functions, including biofilm formation, thus indicating that polyamines may have potential in the control of unwanted biofilm. in the present study, the effects of the polyamines norspermidine and spermidine on biofilms of 10 potentially pathogenic wild-type strains of escherichia coli serotype o103:h2, salmonella enterica subsp. enterica serovar typhimurium, and s. enterica serovar agona were invest ... | 2015 | 25595767 |
| lipopolysaccharides belonging to different salmonella serovars are differentially capable of activating toll-like receptor 4. | salmonella enterica subsp. enterica serovar (serotype) abortusovis is a member of the enterobacteriaceae. this serotype is naturally restricted to ovine species and does not infect humans. limited information is available about the immune response of sheep to s. abortusovis. s. abortusovis, like salmonella enterica subsp. enterica serovar typhi, causes a systemic infection in which, under natural conditions, animals are not able to raise a rapid immune response. failure to induce the appropriate ... | 2014 | 25135686 |
| antibiotic resistance in salmonella typhimurium associates with crispr sequence type. | salmonella enterica subspecies enterica serovar typhimurium is a leading cause of foodborne salmonellosis in the united states. the number of antibiotic resistant isolates identified in humans is steadily increasing, suggesting that the spread of antibiotic resistant strains is a major threat to public health. s. typhimurium is commonly identified in a wide range of animal hosts, food sources, and environments, but little is known about the factors mediating the spread of antibiotic resistance i ... | 2013 | 23796925 |
| a constitutively mannose-sensitive agglutinating salmonella enterica subsp. enterica serovar typhimurium strain, carrying a transposon in the fimbrial usher gene stbc, exhibits multidrug resistance and flagellated phenotypes. | static broth culture favors salmonella enterica subsp. enterica serovar typhimurium to produce type 1 fimbriae, while solid agar inhibits its expression. a transposon inserted in stbc, which would encode an usher for stb fimbriae of a non-flagellar salmonella enterica subsp. enterica serovar typhimurium lb5010 strain, conferred it to agglutinate yeast cells on both cultures. rt-pcr revealed that the expression of the fimbrial subunit gene fima, and fimz, a regulatory gene of fima, were both incr ... | 2012 | 22654583 |
| identification of bapa in strains of salmonella enterica subsp. enterica isolated from wild animals kept in captivity in sinaloa, mexico. | bapa, previously named stm2689, encodes the bapa protein, which, along with cellulose and fimbriae, constitutes biofilms. biofilms are communities of microorganisms that grow in a matrix of exopolysaccharides and may adhere to living tissues or inert surfaces. biofilm formation is associated with the ability to persist in different environments, which contributes to the pathogenicity of several species. we analyzed the presence of bapa in 83 strains belonging to 17 serovars of salmonella enteric ... | 2016 | 27379195 |
| aneurysm of the cranial mesenteric artery as a site of carriage of salmonella enterica subsp. enterica serovar abortusequi in the horse. | salmonella enterica subsp. enterica serovar abortusequi is a pathogen restricted to horses. our investigation targeted 4 draft horses (9-10 months old) kept on a japanese farm that had suffered an outbreak of s. abortusequi abortion. the 4 horses were suspected to be carriers of the bacterium owing to their high agglutination titers (≥1:2,560) in tube agglutination testing. the owners' on-farm observations confirmed that the horses had no apparent abnormalities, and s. abortusequi was not isolat ... | 2016 | 27271985 |
| co-occurrence of free-living protozoa and foodborne pathogens on dishcloths: implications for food safety. | in the present study, the occurrence of free-living protozoa (flp) and foodborne bacterial pathogens on dishcloths was investigated. dishcloths form a potentially important source of cross-contamination with flp and foodborne pathogens in food-related environments. first various protocols for recovering and quantifying flp from dishcloths were assessed. the stomacher technique is recommended to recover flagellates and amoebae from dishcloths. ciliates, however, were more efficiently recovered us ... | 2014 | 25260173 |
| radiation-treated ready-to-eat (rte) chicken breast adobo for immuno-compromised patients. | usually in hospitals low-bacterial diets are served to immuno-compromised patients (icps). however, low-bacterial diets still pose a high risk of microbial infections and limit the food selection of the patients. thus, pathogen-free dishes must be made available. this study presents the development of pathogen-free ready-to-eat (rte) filipino ethnic food chicken breast adobo, sterilized by exposure to high-dose gamma rays (25 kgy) in combination with conventional treatments. frozen vacuum-packed ... | 2014 | 24912709 |
| potential probiotic attributes of a new strain of bacillus coagulans cgmcc 9951 isolated from healthy piglet feces. | a new strain of bacillus coagulans cgmcc 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including escherichia coli o8, staphylococcus aureus, salmonella enterica subsp. enterica serovar enteritidis, streptococcus suis, listeria monocytogenes and pasteurella multocida, was isolated from healthy piglet feces. in adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of b. subtilis jt143 and l. acidophilus ly24 res ... | 2015 | 25752235 |
| collateral damage: insights into bacterial mechanisms that predispose host cells to cancer. | infections are estimated to contribute to 20% of all human tumours. viruses are known to induce cell transformation, but evidence has also linked bacteria, such as helicobacter pylori and salmonella enterica subsp. enterica serovar typhi, to different cancer types. in addition, chlamydia trachomatis, fusobacterium nucleatum and bacteroides fragilis are associated with the development of cancer, although a causal relationship has not yet been established. bacterial effectors such as colibactin an ... | 2017 | 28045107 |
| mortality and translocation assay to study the protective capacity of bifidobacterium lactis inl1 against salmonella typhimurium infection in mice. | the mouse has been largely used for the study of the protective capacity of probiotics against intestinal infections caused by salmonella. in this work we aimed at comparing the mortality and translocation assay for the study of the protective capacity of the human breast milk-derived strain bifidobacterium animalis subsp. lactis inl1 on a model of gut infection by salmonella enterica subsp. enterica serovar typhimurium. different doses of s. typhimurium funed and b. animalis subsp. lactis inl 1 ... | 2014 | 24902954 |
| bifidobacterium thermophilum rbl67 impacts on growth and virulence gene expression of salmonella enterica subsp. enterica serovar typhimurium. | bifidobacterium thermophilum rbl67 (rbl67), a human fecal isolate and health promoting candidate shows antagonistic and protective effects against salmonella and listeria spec. in vitro. however, the underlying mechanisms fostering these effects remain unknown. in this study, the interactions of rbl67 and salmonella enterica subsp. enterica serovar typhimurium n-15 (n-15) were explored by global transcriptional analysis. | 2016 | 26988691 |
| synergistic effects of bifidobacterium thermophilum rbl67 and selected prebiotics on inhibition of salmonella colonization in the swine proximal colon polyferms model. | probiotics and prebiotics are promising strategies to counteract salmonella prevalence in swine. in the present study, we investigated the effects of prebiotics (fructo- (fos), galacto- (gos) and mannan- (mos) oligosaccharides) and the bacteriocinogenic bifidobacterium thermophilum rbl67 (rbl67) on salmonella enterica subsp. enterica serovar typhimurium n-15 (n-15) colonization using the polyferms in vitro continuous fermentation model simulating the swine proximal colon. | 2014 | 25364390 |
| prevalence and characteristics of salmonella and campylobacter in retail poultry meat in japan. | this study was undertaken to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of salmonella enterica subsp. enterica and campylobacter spp. in poultry meat, and to analyze the association of genetic types of these bacteria with their geographical distribution and antimicrobial resistance profiles. fifty-four and 71 of 100 samples were found to be contaminated with salmonella and campylobacter, respectively. nine salmonella serotypes were found, including s. enteric ... | 2016 | 27580577 |
| decay of genetic markers for fecal bacterial indicators and pathogens in sand from lake superior. | beach sands impact water quality and pathogen loads, however, the comparative decay of the fecal indicator bacteria (fib) enterococcus spp. and escherichia coli, and pathogens in freshwater sand have not been examined. in this study, freshwater sand microcosms were inoculated with sewage and pure cultures of bacterial pathogens to compare relative decay rates. the abundance of culturable enterococcus spp. and e. coli, genetic markers for enterococcus spp. (entero1), total bacteroides (allbac), a ... | 2014 | 24793108 |
| a modified molecular beacons-based multiplex real-time pcr assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application. | foodborne disease outbreaks are often caused by one of the major pathogens. early identification of the causal pathogen is crucial for disease control and prevention. we describe a real-time polymerase chain reaction (rtpcr) assay that can identify, in a single reaction, up to eight common foodborne bacterial pathogens, including salmonella enterica subsp. enterica, listeria monocytogenes, escherichia coli o157, vibrio parahaemolyticus, v. vulnificus, campylobacter jejuni, enterobacter sakazakii ... | 2014 | 24328501 |
| prevalence and characterization of foodborne pathogens in dairy cattle in the eastern part of japan. | to investigate the prevalence and characterization of foodborne pathogens [campylobacter spp., shiga toxin-producing escherichia coli (stec), listeria monocytogenes and salmonella spp.] in dairy cows, rectal content grab samples were collected from 250 dairy cows reared on 25 dairy farms in eastern japan from december 2010 through february 2011. campylobacter jejuni was isolated from 106 (42%) cows on 23 (92%) farms, stec o157 from three cows on one farm, l. monocytogenes from three cows on anot ... | 2013 | 23208291 |
| recovery estimation of dried foodborne pathogens is directly related to rehydration kinetics. | drying is a common process which is used to preserve food products and technological microorganisms, but which is deleterious for the cells. the aim of this study is to differentiate the effects of drying alone from the effects of the successive and necessary rehydration. rehydration of dried bacteria is a critical step already studied in starter culture but not for different kinetics and not for pathogens. in the present study, the influence of rehydration kinetics was investigated for three fo ... | 2016 | 27494169 |
| internalisation potential of escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium and staphylococcus aureus in lettuce seedlings and mature plants. | the internalisation potential of listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7 and salmonella enterica subsp. enterica serovar typhimurium in lettuce was evaluated using seedlings grown in vermiculite in seedling trays as well as hydroponically grown lettuce. sterile distilled water was spiked with one of the four human pathogenic bacteria (10(5) cfu/ml) and used to irrigate the plants. the potential for pathogen internalisation was investigated over time using light mi ... | 2013 | 23708570 |
| probiotic properties of lactic acid bacteria isolated from human milk. | the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties. | 2016 | 27159339 |
| characterization of lactobacillus fermentum pl9988 isolated from healthy elderly korean in a longevity village. | in this work, we wanted to develop a probiotic from famous longevity villages in korea. we visited eight longevity villages in korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. isolated colonies that appeared on mrs agar containing bromophenol blue were identified by means of 16s rrna sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 ... | 2015 | 26095384 |
| prevalence of the aminoglycoside phosphotransferase genes aph(3')-iiia and aph(3')-iia in escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus aureus isolates in austria. | the aminoglycoside phosphotransferase aph(3')-iia primarily inactivates kanamycin and neomycin, whilst aph(3')-iiia also inactivates amikacin. the aim of this study was to determine the frequency of both resistance genes in major human pathogens to obtain their baseline prevalence in the gene pool of these bacterial populations in austria. in total, 10 541 escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus ... | 2014 | 24194558 |
| immunostimulatory effects of recombinant erysipelothrix rhusiopathiae expressing porcine interleukin-18 in mice and pigs. | interleukin-18 (il-18), which was originally called gamma interferon (ifn-γ)-inducing factor, has been shown to play an important role in innate and acquired immune responses. in this study, attenuated erysipelothrix rhusiopathiae strains were engineered to produce porcine il-18 (poil-18) and evaluated for their potential immunostimulatory effect in animals. recombinant poil-18 was successfully expressed in the recombinant e. rhusiopathiae strains ys-1/il-18 and ko/il-18. the culture supernatant ... | 2012 | 22761300 |
| detection of brucellosis in sika deer ( cervus nippon ) through loop-mediated isothermal amplification (lamp). | brucellosis (brucella bovis) in sika deer ( cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. it also poses a threat to humans because it is a zoonotic disease. use of the loop-mediated isothermal amplification (lamp) assay has been poorly described in the diagnosis of brucellosis in deer. we developed a lamp assay targeting the omp25 gene sequence to detect brucellosis (brucella bovis) in sika deer. the react ... | 2017 | 28318383 |
| evaluation of molecular typing of foodborne pathogens in european reference laboratories from 2012 to 2013. | in 2012, the european centre for disease prevention and control (ecdc) initiated external quality assessment (eqa) schemes for molecular typing including the national public health reference laboratories in europe. the overall aim for these eqa schemes was to enhance the european surveillance of food-borne pathogens by evaluating and improving the quality and comparability of molecular typing. the eqas were organised by statens serum institut (ssi) and included salmonella enterica subsp. enteric ... | 2016 | 28006653 |
| metagenomics of two severe foodborne outbreaks provides diagnostic signatures and signs of coinfection not attainable by traditional methods. | diagnostic testing for foodborne pathogens relies on culture-based techniques that are not rapid enough for real-time disease surveillance and do not give a quantitative picture of pathogen abundance or the response of the natural microbiome. powerful sequence-based culture-independent approaches, such as shotgun metagenomics, could sidestep these limitations and potentially reveal a pathogen-specific signature on the microbiome that would have implications not only for diagnostics but also for ... | 2017 | 27881416 |
| whole-genome sequencing identifies in vivo acquisition of a blactx-m-27-carrying incfii transmissible plasmid as the cause of ceftriaxone treatment failure for an invasive salmonella enterica serovar typhimurium infection. | we report a case of ceftriaxone treatment failure for bacteremia caused by salmonella enterica subsp. enterica serovar typhimurium, due to the in vivo acquisition of a blactx-m-27-encoding incfii group transmissible plasmid. the original β-lactamase-susceptible isolate st882s was replaced by the resistant isolate st931r during ceftriaxone treatment. after relapse, treatment was changed to ciprofloxacin, and the patient recovered. isolate st931r could transfer resistance to escherichia coli at 37 ... | 2016 | 27671066 |
| detection of a novel qnrb19-carrying plasmid variant mediating decreased fluoroquinolone susceptibility in salmonella enterica serovar hadar. | thirty salmonella enterica subsp. enterica serovar hadar isolates of avian origin collected between 2007 and 2010 from chicken carcasses in five geographically spread abattoirs in germany were investigated for plasmid-mediated quinolone resistance determinants. four isolates were identified by pcr analysis and hybridization experiments to carry qnrb genes. the isolates were indistinguishable by their xbai macrorestriction patterns and did not exhibit a mutation in the quinolone resistance-determ ... | 2017 | 27404660 |
| loop-to-helix transition in the structure of multidrug regulator acrr at the entrance of the drug-binding cavity. | multidrug transcription regulator acrr from salmonella enterica subsp. enterica serovar typhimurium str. lt2 belongs to the tetracycline repressor family, one of the largest groups of bacterial transcription factors. the crystal structure of dimeric acrr was determined and refined to 1.56å resolution. the tertiary and quaternary structures of acrr are similar to those of its homologs. the multidrug binding site was identified based on structural alignment with homologous proteins and has a di(hy ... | 2016 | 26796657 |
| label-free mapping of single bacterial cells using surface-enhanced raman spectroscopy. | here we presented a simple, rapid and label-free surface-enhanced raman spectroscopy (sers) based mapping method for the detection and discrimination of salmonella enterica and escherichia coli on silver dendrites. the sample preparation was first optimized to maximize sensitivity. the mapping method was then used to scan through the bacterial cells adsorbed on the surface of silver dendrites. the intrinsic and distinct sers signals of bacterial cells were used as the basis for label-free detect ... | 2016 | 26750611 |
| whole genome sequencing provides possible explanations for the difference in phage susceptibility among two salmonella typhimurium phage types (dt8 and dt30) associated with a single foodborne outbreak. | phage typing has been used for decades as a rapid, low cost approach for the epidemiological surveillance of salmonella enterica subsp. enterica serovar typhimurium. although molecular methods are replacing phage typing the system is still in use and provides a valuable model for study of phage-host interaction. phage typing depends on the pattern of bacterial resistance or sensitivity to a panel of specific bacteriophages. in the phage typing scheme, s. typhimurium definitive phage types (dt) 8 ... | 2015 | 26613761 |
| removal of pathogenic bacterial biofilms by combinations of oxidizing compounds. | bacterial biofilms are commonly formed on medical devices and food processing surfaces. the antimicrobials used have limited efficacy against the biofilms; therefore, new strategies to prevent and remove these structures are needed. here, the effectiveness of brief oxidative treatments, based on the combination of sodium hypochlorite (naclo) and hydrogen peroxide (h2o2) in the presence of copper sulfate (cuso4), were evaluated against bacterial laboratory strains and clinical isolates, both in p ... | 2015 | 25864510 |
| partition locus-based classification of selected plasmids in klebsiella pneumoniae, escherichia coli and salmonella enterica spp.: an additional tool. | the dissemination of antimicrobial resistance genes in enterobacteriaceae has been largely attributed to plasmids, circular dna molecules capable of autonomous replication. whereas high-copy-number plasmids primarily rely on passive diffusion for plasmid maintenance, low-copy-number plasmids utilize so-called partition (par) systems. plasmid partition relies on three structures, i.e. a centromere like dna site, a centromere-binding protein and an atpase or a gtpase motor protein for plasmid posi ... | 2015 | 25623509 |
| depuration times of donax trunculus and tapes decussatus. | the present study was performed to determine the depuration time and ability of donax trunculus (wedge clam) and tapes decussatus (carpet shell) contaminated with escherichia coli, salmonella enterica subsp. enterica serovar typhimurium and vibrio parahaemolyticus. clams were contaminated with each bacterium at the level of 7.0 - 8.0 log10 cfu/g. after contamination, clams were analyzed every 3 h in the first 24 h time period and every 6 h until the 72nd hour. during the depuration process of bo ... | 2014 | 25477938 |
| investigating the physiological roles of low-efficiency d-mannonate and d-gluconate dehydratases in the enolase superfamily: pathways for the catabolism of l-gulonate and l-idonate. | the sequence/function space in the d-mannonate dehydratase subgroup (mand) of the enolase superfamily was investigated to determine how enzymatic function diverges as sequence identity decreases [wichelecki, d. j., et al. (2014) biochemistry 53, 2722-2731]. that study revealed that members of the mand subgroup vary in substrate specificity and catalytic efficiency: high-efficiency (kcat/km = 10(3)-10(4) m(-1) s(-1)) for dehydration of d-mannonate, low-efficiency (kcat/km = 10-10(2) m(-1) s(-1)) ... | 2014 | 25145794 |
| development of ssdna aptamers for the sensitive detection of salmonella typhimurium and salmonella enteritidis. | salmonella enterica subsp. enterica ser. enteritidis and salmonella enterica subsp. enterica ser. typhimurium are the most common and severe food-borne pathogens responsible for causing salmonellosis in humans and animals. the development of an early and ultra-sensitive detection system is the first critical step in controlling this disease. to accomplish this, we used the cell systematic evolution of ligands by exponential enrichment (cell-selex) technique to identify single-stranded dna (ssdna ... | 2014 | 25096391 |
| targeting relaxase genes for classification of the predominant plasmids in enterobacteriaceae. | plasmids are the main vectors of antimicrobial drug resistance and virulence genes, especially in enterobacteriaceae. identification and classification of plasmids is essential for analysis of their distribution. the most widely used typing method is pcr-based replicon typing (pbrt). a new classification scheme based on relaxase gene typing has been described recently. we propose a practical application of this method, with the development of a multiplex pcr set targeting relaxase genes found on ... | 2014 | 24342269 |
| combining de novo and reference-guided assembly with scaffold_builder. | genome sequencing has become routine, however genome assembly still remains a challenge despite the computational advances in the last decade. in particular, the abundance of repeat elements in genomes makes it difficult to assemble them into a single complete sequence. identical repeats shorter than the average read length can generally be assembled without issue. however, longer repeats such as ribosomal rna operons cannot be accurately assembled using existing tools. the application scaffold_ ... | 2013 | 24267787 |
| bioprotection of ready-to-eat probiotic artichokes processed with lactobacillus paracasei lmgp22043 against foodborne pathogens. | the survival of 3 pathogens listeria monocytogenes atcc19115, salmonella enterica subsp. enterica atcc13311, and escherichia coli atcc8739 was evaluated over time in ready-to-eat (rte) artichoke products processed or not with the probiotic strain lactobacillus paracasei lmgp22043. both probiotic and standard products (final ph about 4.0; aw = 0.98) dressed with oil and packaged in modified atmosphere were inoculated with pathogens at a level of about 3 log cfu/g and stored at 4 ºc for 45 d. path ... | 2013 | 24245894 |
| extracellular proteins from lactobacillus plantarum bmcm12 prevent adhesion of enteropathogens to mucin. | the aim of this study was to study the interference of the extracellular proteins produced by lactobacillus plantarum bmcm12 with the adhesion of some well-known gut pathogens. the extracellular proteins secreted by l. plantarum bmcm12 in mrs broth were precipitated, resolved by sds-page, and identified by tandem mass spectrometry. discordances between the observed and the theoretical molecular masses of several proteins suggested the presence of protein glycosylation, corroborated with specific ... | 2012 | 22461079 |
| anti-salmonella activity and probiotic trends of kluyveromyces marxianus s-2-05 and kluyveromyces lactis s-3-05 isolated from a french cheese, tomme d'orchies. | kluyveromyces marxianus s-2-05 and kluyveromyces lactis s-3-05 were recently isolated from a traditional french cheese, tomme d'orchies, and characterized here for their advantages using a different application perspective. first, we established their anti-salmonella activity and downregulation of the virulence sopd gene of salmonella enterica subsp. enterica serovar typhimurium, mainly in the presence of k. marxianus s-2-05. in addition to their antagonism, these non-saccharomyces yeasts were a ... | 2017 | 28366839 |
| non-saccharomyces yeasts protect against epithelial cell barrier disruption induced by salmonella enterica subsp. enterica serovar typhimurium. | the human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. whereas invasive pathogens cause epithelial barrier disruption, probiotic micro-organisms modulate tight junction regulation and improve epithelial barrier function. in addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. the aim of this study was to explore non-saccharomyces yeast modulation of epithelial cell barrier func ... | 2015 | 26280244 |
| isolation, identification, and evaluation of novel probiotic strains isolated from feces of breast-fed infants. | to isolate, identify, and evaluate the probiotic properties of lactic acid bacteria (lab) isolated from the feces of breast-fed infants. | 2016 | 26817236 |
| effect of temperature and nutrient concentration on survival of foodborne pathogens in deciduous fruit processing environments for effective hygiene management. | temperature and good sanitation practices are important factors for controlling growth of microorganisms. fresh produce is stored at various temperatures to ensure quality and to prolong shelf life. when foodborne pathogens survive and grow on fresh produce at storage temperatures, then additional control strategies are needed to inactivate these pathogens. the aim of this study was to determine how temperatures associated with deciduous fruit processing and storage facilities (0.5, 4, and 21°c) ... | 2016 | 28221909 |
| pectin and xyloglucan influence the attachment of salmonella enterica and listeria monocytogenes to bacterial cellulose-derived plant cell wall models. | minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. these pathogens must attach to the produce in order to be transmitted. cut surfaces of produce that expose cell walls are particularly vulnerable. little is known about the roles that different structural components (cellulose, pectin, and xyloglucan) of plant cell walls play in the attachment of foodborne bacterial pathogens. using bacterial cellulose-derived plant cell wall models ... | 2015 | 26567310 |
| natural antimicrobials and high-pressure treatments on the inactivation of salmonella enteritidis and escherichia coli o157:h7 in cold-smoked salmon. | high hydrostatic pressure (hhp) combined with reuterin and lactoperoxidase system (lps) has exerted antimicrobial activity against listeria monocytogenes in cold-smoked salmon at chilled temperatures. therefore the purpose of this work was to evaluate the effect of hhp combined with reuterin, lps and lactoferrin (lf) on the survival of salmonella enterica subsp. enterica serovar enteritidis and escherichia coli o157:h7 in cold-smoked salmon stored at 4 and 10 °c. | 2016 | 26268416 |
| combined treatments of high-pressure with the lactoperoxidase system or lactoferrin on the inactivation of listeria monocytogenes, salmonella enteritidis and escherichia coli o157:h7 in beef carpaccio. | the effect of high hydrostatic pressure (hhp) treatments in combination with the lactoperoxidase system (lpos) or activated lactoferrin (alf) on listeria monocytogenes, salmonella enterica subsp. enterica serovar enteritidis and escherichia coli o157:h7 was investigated in cured beef carpaccio stored at 8 °c or 22 °c during 7 d. hhp (450 mpa for 5 min) reduced pathogen levels by 1-3 log units and the antimicrobial effect remained during 7 d of storage under temperature abuse conditions at 8 °c a ... | 2014 | 24750810 |
| evaluation of aerated steam treatment of alfalfa and mung bean seeds to eliminate high levels of escherichia coli o157:h7 and o178:h12, salmonella enterica, and listeria monocytogenes. | sprouts contaminated with human pathogens are able to cause food-borne diseases due to the favorable growth conditions for bacteria during germination and because of minimal processing steps prior to consumption. we have investigated the potential of hot humid air, i.e., aerated steam, to treat alfalfa and mung bean seeds which have been artificially contaminated with escherichia coli o157:h7, salmonella enterica subsp. enterica serovar weltevreden, and listeria monocytogenes scott a. in additio ... | 2013 | 23709507 |
| shedding of zoonotic pathogens and analysis of stomach contents in great cormorants (phalacrocorax carbo sinensis) from switzerland between 2007 and 2012. | 208 healthy great cormorants (phalacrocorax carbo sinensis) shot during 5 consecutive hunting seasons from 2007/2008 until 2011/2012 were tested for newcastle disease virus (apmv-1), avian influenza virus (aiv), chlamydiae, and salmonella spp. in addition, stomach contents were gross macroscopically examined. none of the birds was positive for apmv1, aiv or chlamydiae. twice salmonella enterica subsp. enterica serovar typhimurium and once a rough mutant of salmonella typhimurium were found. stom ... | 2014 | 25082636 |
| saccharomyces boulardii prevention of the hepatic injury induced by salmonella enteritidis infection. | salmonella enterica subsp. enterica serovar enteritidis (salmonella enteritidis) is the predominant cause of serovar-associated food-borne outbreaks in many countries and causes significant clinical symptoms of liver injury, enteritis, and diarrheal diseases. saccharomyces boulardii is used in clinical application for prophylaxis and the treatment of a variety of diseases caused by bacterial infection. we used a mouse model of salmonella enteritidis infection, which included pretreatment with s. ... | 2014 | 25231732 |
| whole-genome comparative analysis of salmonella enterica serovar newport strains reveals lineage-specific divergence. | salmonella enterica subsp. enterica serovar newport has been associated with various foodborne outbreaks in humans and animals. phylogenetically, serovar newport is one of several salmonella serovars that are polyphyletic. to understand more about the polyphyletic nature of this serovar, six food, environment, and human isolates from different newport lineages were selected for genome comparison analyses. whole genome comparisons demonstrated that heterogeneity mostly occurred in the prophage re ... | 2017 | 28379364 |
| comparative subproteomic analysis of clinically acquired fluoroquinolone resistance and ciprofloxacin stress in salmonella typhimurium dt104b. | antimicrobial resistance is a worldwide public health threat and salmonella enterica subsp. enterica serotype typhimurium phage type dt104b multiresistant strains with additional quinolone resistance have been responsible for global outbreaks and high mortality. quinolone resistance is known to be multifactorial but is still far from a complete understanding. to give new insights about the resistance mechanisms involved, this work aimed to evaluate subproteome changes between a s. typhimurium dt ... | 2017 | 28314077 |
| complete genome and methylome sequences of salmonella enterica subsp. enterica serovars typhimurium, saintpaul, and stanleyville from the sara/sarb collection. | in this announcement, we report the complete genome and methylome sequences of three salmonella enterica strains from the sara and sarb collection: s. enterica subsp. enterica serovar typhimurium (sara13), s. enterica subsp. enterica serovar saintpaul (sara26), and s. enterica subsp. enterica serovar stanleyville (sarb61). | 2017 | 28302778 |
| kidney allograft pyelonephritis caused by salmonella enterica serovar schwarzengrund. | kidney transplant recipients (ktrs) taking immunosuppressive drugs have a 20-fold greater risk of nontyphoidal salmonella (nts) infection than the healthy adult population. among ktrs, the urinary tract is the most common site of infection. however, few cases of urinary tract infection caused by nts have been documented in ktrs, and only one in japan. furthermore, it frequently induces acute allograft rejection with high mortality. salmonella enterica subsp. enterica serovar schwarzengrund (s. s ... | 2017 | 28302440 |
| genome sequence of the thermotolerant foodborne pathogen salmonella enterica serovar senftenberg atcc 43845 and phylogenetic analysis of loci encoding increased protein quality control mechanisms. | salmonella enterica subsp. enterica bacteria are important foodborne pathogens with major economic impact. some isolates exhibit increased heat tolerance, a concern for food safety. analysis of a finished-quality genome sequence of an isolate commonly used in heat resistance studies, s. enterica subsp. enterica serovar senftenberg 775w (atcc 43845), demonstrated an interesting observation that this strain contains not just one, but two horizontally acquired thermotolerance locus homologs. these ... | 2017 | 28293682 |
| mlva for salmonella enterica subsp. enterica serovar dublin: development of a method suitable for inter-laboratory surveillance and application in the context of a raw milk cheese outbreak in france in 2012. | salmonella enterica subspecies enterica serovar dublin (s. dublin) figures among the most frequently isolated salmonella strains in humans in france. this serovar may affect production and animal health mainly in cattle herds with corresponding high economic losses. given that the current gold standard method, pulsed-field gel electrophoresis (pfge), provides insufficient discrimination for epidemiological investigations, we propose a standard operating procedure in this study for multiple-locus ... | 2017 | 28289408 |
| draft genome sequences of 18 salmonella enterica subsp. enterica serovar oranienburg strains isolated from rivers in northwestern mexico. | salmonella enterica subsp. enterica serovar oranienburg is recognized as a foodborne pathogen widely distributed in the environment. here, we report 18 draft genomes of s oranienburg strains isolated from rivers in the northwestern region of mexico. | 2017 | 28280020 |
| genome sequences of salmonella enterica subsp. enterica serovar infantis strains from hungary representing two peak incidence periods in three decades. | four strains of salmonella enterica subsp. enterica serovar infantis isolated from humans (1980 to 1982) and broiler chickens (2016) have been sequenced. they represent the early and recent peak incidences of this serovar in hungary. genome sequences of these isolates provide comparative data on the evolution and rise of an endemic s infantis clone in hungary. | 2017 | 28254986 |
| development and validation of a cultural method for the detection and isolation of salmonella in cloves. | detection of salmonella in some spices, such as cloves, remains a challenge due to their inherent antimicrobial properties. the purpose of this study was to develop an effective detection method for salmonella from spices using cloves as a model. two clove varieties, ceylon and madagascar, were used in the study. cloves were inoculated with salmonella enterica subsp. enterica serotypes montevideo, typhimurium, or weltevreden at about 1, 3, or 6 log cfu/25 g. two test portion sizes, 10 and 25 g, ... | 2017 | 28199150 |
| rapid concentration detection and differentiation of bacteria in skimmed milk using surface enhanced raman scattering mapping on 4-mercaptophenylboronic acid functionalized silver dendrites. | a novel method was developed to rapidly concentrate, detect, and differentiate bacteria in skimmed milk using surface enhanced raman scattering (sers) mapping on 4-mercaptophenylboronic acid (4-mpba) functionalized silver (ag) dendrites. the 4-mpba functionalized ag dendritic sers substrate was used to capture the bacterial cells and enhance the bacterial signal. salmonella, a significantly important food pathogen, was used as the representative strain to optimize and evaluate the developed meth ... | 2017 | 28091716 |
| fowl typhoid in laying hens cause hepatic oxidative stress. | the aim of this study was to analyses nitric oxide, antioxidant status, and oxidative profile in the liver of laying hens naturally infected by salmonella enterica subsp enterica serovar gallinarum (s. gallinarum). the nitrite/nitrate (nox), reactive oxygen species (ros), thiobarbituric acid-reactive substances (tbars), catalase (cat), glutathione reductase (gr), glutathione peroxidase (gpx) and glutathione s-transferase (gst) activities were measured in liver samples, as well the biomarkers of ... | 2017 | 28027943 |
| draft genome sequence of a multidrug-resistant cmy-2-producing salmonella enterica subsp. enterica serovar minnesota st3088 isolated from chicken meat. | | 2017 | 28017672 |