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competition and coexistence in a small carnivore guild.the potential for strong competition among small sympatric carnivores results in a need for coexistence strategies whereby competitors partition along spatial, temporal and dietary axes as a means to reduce ecological overlaps. we determined spatial and temporal partitioning patterns of a guild of small african carnivores: the african wildcat felis silvestris lybica, grey mongoose galerella pulverulenta, small-spotted genet genetta genetta, striped polecat ictonyx striatus, and the yellow mongoo ...201728733835
alterations in selected serum biochemistry values of free-ranging bornean leopard cats (prionailurus bengalensis borneoensis) captured by box traps.box-traps for capturing wild cats are widely used by researchers since it is one of the most effective methods for trapping these species. although they are extensively utilised, the effects on the physiology of trapped felids remain unclear. researchers frequently make judgements regarding the safety of such capture devices by examination of external injuries but often fail to take into consideration other physiological parameters. to assess the effects of capture events on selected serum bioch ...201424839119
natural pet food: a review of natural diets and their impact on canine and feline physiology.the purpose of this review is to clarify the definition of "natural" as it pertains to commercial pet food and to summarize the scientific findings related to natural ingredients in pet foods and natural diets on the impact of pet health and physiology. the term "natural," when used to market commercial pet foods or pet food ingredients in the united states, has been defined by the association of american feed control officials and requires, at minimum, that the pet food be preserved with natura ...201425006071
[major histocompatibility complex (mhc) in mammals' and its importance for studies of rare species (with felidae family as an example)].major histocompatibility complex (mhc) appears to be a suitable tool for solving various tasks of popu- lationgenetics. information on genetic basis of immunity facilitates understanding of evolutionary his- tory and assessment of current state and prospects of studied population/species survival. on the one hand, mhc variability is maintained through pathogen dependent mechanisms, i.e., directional selection of individuals resistant to diseases, that are present in the environment and balancing ...201525786311
nanog is an essential factor for induction of pluripotency in somatic cells from endangered felids.nanog has an important role in pluripotency induction in bovines and snow leopards. to examine whether it was required for wild felids globally, we examined the induction of pluripotency in felids from asia (bengal tiger, panthera tigris), africa (serval, leptailurus serval), and the americas (jaguar, panthera onca). dermal fibroblasts were transduced with genes encoding the human transcription factors oct4, sox2, klf4, and cmyc with or without nanog. both four- and five-factor induction resulte ...201323514873
on a species of babesia in an indian wild cat (felis catus). 195024537581
comparative assessment of genetic and morphological variation at an extensive hybrid zone between two wild cats in southern brazil.increased attention towards the neotropical cats leopardus guttulus and l. geoffroyi was prompted after genetic studies identified the occurrence of extensive hybridization between them at their geographic contact zone in southern brazil. this is a region where two biomes intersect, each of which is associated with one of the hybridizing species (atlantic forest with l. guttulus and pampas with l. geoffroyi). in this study, we conducted in-depth analyses of multiple molecular markers aiming to c ...201425250657
molecular data reveal complex hybridization and a cryptic species of neotropical wild cat.hybridization among animal species has recently become more recognized as an important phenomenon, especially in the context of recent radiations. here we show that complex hybridization has led to contrasting patterns of genomic composition among closely related species of the neotropical cat genus leopardus. we show strong evidence of ancient hybridization and introgression between the pampas cat (l. colocolo) and northeastern populations of tigrina (l. tigrinus), leading to remarkable cytonuc ...201324291091
laparoscopic oviductal embryo transfer and artificial insemination in felids--challenges, strategies and successes.embryo transfer (et) and artificial insemination (ai) are potentially invaluable techniques for the propagation and management of genetically valuable domestic cat and endangered nondomestic cat populations. many of the challenges that impair the effective application of et and ai in felids may be overcome by using laparoscopic oviductal (lo) approaches. lo-et and lo-ai are minimally-invasive procedures, requiring only two small skin incisions for insertion of a laparoscope and grasping forceps ...201223279483
nonregenerative immune-mediated anemia associated with a diffuse large b-cell lymphoma in a captive jaguar (panthera onca).an 18-year-old male castrated jaguar (panthera onca) was presented with anorexia and continuous bleeding from the oral cavity after a history of fighting with the partner animal. clinical evaluation revealed ulcerating lesions on the gingiva and hard palate and a hematoma on the tongue. computed tomography of the head and endoscopic examination of the esophagus and stomach were unremarkable. hematology and clinical chemistry revealed severe nonregenerative anemia, mild thrombocytopenia, and mode ...201728902958
facultative myiasis of domestic cats by sarcophaga argyrostoma (diptera: sarcophagidae), calliphora vicina and lucilia sericata (diptera: calliphoridae) in northern italy.we describe five cases of myiasis of domestic cats, felis silvestris catus l. (carnivora: felidae), reported in 2016 in northern italy and caused by three diptera species: sarcophaga argyrostoma (robineau-desvoidy) (sarcophagidae), calliphora vicina robineau-desvoidy and lucilia sericata (meigen) (calliphoridae). three were cases of traumatic myiasis, one by s. argyrostoma and two by l. sericata, one was a case of auricular myiasis by c. vicina and one was a case of ophthalmomyiasis caused by an ...201728803354
scotopic electroretinography in fishing cat (prionailurus viverrinus) and leopard cat (prionailurus bengalensis).to establish baseline normal scotopic electroretinograpic (erg) parameters for two wild cat species: fishing cats (fc) and leopard cats (lc).201727471166
ocelot (leopardus pardalis) density in central amazonia.ocelots (leopardus pardalis) are presumed to be the most abundant of the wild cats throughout their distribution range and to play an important role in the dynamics of sympatric small-felid populations. however, ocelot ecological information is limited, particularly for the amazon. we conducted three camera-trap surveys during three consecutive dry seasons to estimate ocelot density in amanã reserve, central amazonia, brazil. we implemented a spatial capture-recapture (scr) model that shared det ...201627191598
the first estimates of marbled cat pardofelis marmorata population density from bornean primary and selectively logged forest.the marbled cat pardofelis marmorata is a poorly known wild cat that has a broad distribution across much of the indomalayan ecorealm. this felid is thought to exist at low population densities throughout its range, yet no estimates of its abundance exist, hampering assessment of its conservation status. to investigate the distribution and abundance of marbled cats we conducted intensive, felid-focused camera trap surveys of eight forest areas and two oil palm plantations in sabah, malaysian bor ...201627007219
molecular identification of the trematode paragonimus in faecal samples from the wild cat prionailurus bengalensis in the da krong nature reserve, vietnam.conventional identification of paragonimus species and their natural definitive hosts is based on the morphological features of adult parasites isolated from the lungs of wild mammalian hosts. however, wild animals are protected by strict regulations and sampling is not always possible. recently, molecular techniques have been developed to identify the internal transcribed spacer (its) sequences of paragonimus eggs in faeces/sputum of human patients. also, mammalian hosts can be identified using ...201626388560
identification and characterization of diverse groups of endogenous retroviruses in felids.endogenous retroviruses (ervs) are genetic elements with a retroviral origin that are integrated into vertebrate genomes. in felids (mammalia, carnivora, felidae), ervs have been described mostly in the domestic cat, and only rarely in wild species. to gain insight into the origins and evolutionary dynamics of endogenous retroviruses in felids, we have identified and characterized partial pro/pol erv sequences from eight neotropical wild cat species, belonging to three distinct lineages of felid ...201525808580
compound mechanism of fatal neck injury: a case report of a tiger attack in a zoo.fatal injuries caused by attacks by large wild cats are extremely rare in forensic medical practice in europe. there are very few cases described in the forensic medical literature concerning incidents in zoos similar to the tiger attack on a 58-year-old male zoo employee that we present here. while preparing a runway for tigers, the man was attacked by a male sumatran tiger. another zoo employee was an eyewitness to the accident; in his testimony he described the sequence of events in detail. t ...201728579157
cell cycle synchronization and analysis of apoptosis-related gene in skin fibroblasts from domestic cat (felis silvestris catus) and kodkod (leopardus guigna).the kodkod population is in constant decrease and the somatic cell nuclear transfer (scnt) might help to preserve the genetic pool of this species. the cell cycle synchronization of donor cells plays a crucial role in scnt. the objective of this research was to evaluate two different methods for quiescence induction, serum starvation (ss) and contact inhibition (ci), both for 1, 3 and 5 days, on skin fibroblast from domestic cat and kodkod. flow cytometry analysis revealed that in domestic cat, ...201728544240
can but don't: olfactory discrimination between own and alien offspring in the domestic cat.mammalian maternal care usually comes at a large energetic cost. to maximize their fitness, mothers should preferentially care for their own offspring. however, the majority of studies of mother-offspring recognition have focused on herd- or colony-living species and there is little information on maternal discrimination in more solitary-living species. olfaction has been found to play a major role in mother-offspring recognition across various taxa. therefore, our aim was to study this in a spe ...201728540504
inconsistency of allometric scaling for dissociative anesthesia of wild felids.to evaluate allometric scaling for ketamine-xylazine (kx) anesthesia in wild felids using domestic cats for reference.201626376437
bobcats (lynx rufus) are natural definitive host of besnoitia darlingi.bovine besnoitiosis, caused by besnoitia besnoiti, is an economically important disease of cattle in many countries but its transmission remains a mystery. wild felids are suspected to be its definitive hosts. the domestic cat (felis catus) is known experimental definitive host for besnoitia species of rodents. here, we report for besnoitia darlingi the first identification of a natural definitive host, the bobcat (lynx rufus). oocysts resembling toxoplasma gondii (unsporulated; 10.9±0.8×12.1±0. ...201729173547
intraspecific variation in the domestic cat bony labyrinth revealed by different measurement techniques.the knowledge of intraspecific variation is important to make assumptions on an interspecific level. to study intraspecific variation in the bony labyrinth morphology of the domestic cat, eleven specimens of felis silvestris catus and two additional subspecies (f. s. lybica, f. s. ornata) were investigated. the sample comprises skulls of adult males and females, as well as juvenile cats. each bony labyrinth endocast was virtually reconstructed based on µct scans. to estimate the radius of curvat ...201829194713
cats and owners interact more with each other after a longer duration of separation.little is known about the cat's (felis silvestris catus) need for human contact, although it is generally believed that cats are more independent pets than e.g. dogs. in this study, we investigated the effect of time left alone at home on cat behaviour (e.g. social and distress-related) before, during and after separation from their owner. fourteen privately owned cats (single-housed) were each subjected to two treatments: the cat was left alone in their home environment for 30 min (t0.5) and fo ...201729045424
specifying and sustaining pigmentation patterns in domestic and wild cats.color markings among felid species display both a remarkable diversity and a common underlying periodicity. a similar range of patterns in domestic cats suggests a conserved mechanism whose appearance can be altered by selection. we identified the gene responsible for tabby pattern variation in domestic cats as transmembrane aminopeptidase q (taqpep), which encodes a membrane-bound metalloprotease. analyzing 31 other felid species, we identified taqpep as the cause of the rare king cheetah pheno ...201222997338
temperament in domestic cats: a review of proximate mechanisms, methods of assessment, its effects on human-cat relationships, and one welfare.temperament can be defined as interindividual differences in behavior that are stable over time and in different contexts. the terms 'personality', 'coping styles', and 'behavioral syndromes' have also been used to describe these interindividual differences. in this review, the main aspects of cat temperament research are summarized and discussed, based on 43 original research papers published between 1986 and 2020. we aimed to present current advances in cat temperament research and identify po ...202032867072
molecular and serological survey of the cat-scratch disease agent (bartonella henselae) in free-ranging leopardus geoffroyi and leopardus wiedii (carnivora: felidae) from pampa biome, brazil.the genus bartonella comprises emerging bacteria that affect humans and other mammals worldwide. felids represent an important reservoir for several bartonella species. domestic cats are the main reservoir of bartonella henselae, the agent of cat scratch disease (csd). it can be transmitted directly by scratches and bites from infected cats and via cat fleas. this study aims to investigate the circulation of bartonella spp. in free-ranging neotropical wild felids from southern brazil using serol ...202032974747
high diversity of blood-associated parasites and bacteria in european wild cats in bosnia and herzegovina: a molecular study.wild felids may play a significant role in the transmission of various pathogens to domestic cats, in particular, but also to other animals and humans. however, since data on the occurrence of blood-borne organisms in european wild cats (felis silvestris silvestris) are scarce, the present study aimed to provide an insight into the genetic diversity of the agents carried by this sylvatic species in bosnia and herzegovina and to elucidate their pathogenic impact. tissue samples from 18 adult wild ...201829422447
evaluation of mercury contamination in iranian wild cats through hair analysis.due to its environmental persistence and bioaccumulative properties, mercury is considered highly toxic to humans, ecosystems, and wildlife. the present study aimed to investigate the total mercury (hg) concentrations in hair samples of 40 wild cats belonging to eight different species collected from various provinces of iran and to characterize their risk of hg exposure. total hg levels in iranian wild cats ranged from 62 to 3670 ng/g dw hair, with a median value of 488 ng/g dw hair. the lowest ...202032306286
exploring the gaps in practical ethical guidance for animal welfare considerations of field interventions and innovations targeting dogs and cats.domestic dogs (canis lupus familiaris) and cats (felis silvestris catus) are common species targeted by nongovernmental or intergovernmental organizations, veterinarians and government agencies worldwide, for field interventions (e.g., population management, rabies vaccination programs) or innovations (e.g., development of technologies or pharmaceuticals to improve animal welfare). we have a moral responsibility to ensure that the conduct of this work is humane for dogs or cats, and to consider ...201829382052
testing aggressive behaviour in a feeding context: importance of ethologically relevant stimuli.the choice of stimuli used in tests of animal behaviour can have a critical effect on the outcome. here we report two experiments showing how different foods influence aggressive behaviour in competition tests at weaning among littermates of the domestic cat. whereas in experiment 1 canned food elicited almost no overt competition, a piece of raw beef rib elicited clearly aggressive behaviour among littermates. in experiment 2 the food stimuli were chosen to differ from raw beef rib in various c ...201829454644
a comparison of cats (felis silvestris catus) housed in groups and single cages at a shelter: a retrospective matched cohort study.the merits of various housing options for domestic cats in shelters have been debated. however, comparisons are difficult to interpret because cats are typically not able to be randomly assigned to different housing conditions. in the current study, we attempted to address some of these issues by creating a retrospective matched cohort of cats in two housing types. cats in group housing (gh) were matched with cats in single housing (sh) that were the same age, sex, breed, coat color, and size. a ...201829443953
[investigation on epidemic foci of paragonimiasis in eastern part of zhenghe county, fujian province].to investigate the species and hosts of paragonimus and its infection rate in eastern part of zhenghe county, fujian province, so as to determine the local foci of paragonimus.201629376284
molecular detection and identification of bartonella in the cat flea ctenocephalides felis felis collected from companion animals in a border area in northeastern argentina.molecular methods were used to detect and identify bartonella species in the cat fleas ctenocephalides felis felis from puerto iguazú, a border area in northeastern argentina. the fleas were collected from 12 household animals, 9 dogs (canis lupus familiaris) and 3 cats (felis silvestris catus) during july 2016. out of 15c. f. felis analyzed for pcr, only one flea collected from a cat was positive (6.66%) in screened for bartonella spp. based on the glta gene. bartonella clarridgeiae was identif ...202032057388
a single-parasite transcriptional atlas of toxoplasma gondii reveals novel control of antigen expression.toxoplasma gondii, a protozoan parasite, undergoes a complex and poorly understood developmental process that is critical for establishing a chronic infection in its intermediate hosts. here, we applied single-cell rna-sequencing (scrna-seq) on >5,400 toxoplasma in both tachyzoite and bradyzoite stages using three widely studied strains to construct a comprehensive atlas of cell-cycle and asexual development, revealing hidden states and transcriptional factors associated with each developmental ...202032065584
genetic characterization of carnivore parvoviruses in spanish wildlife reveals domestic dog and cat-related sequences.the impact of carnivore parvovirus infection on wild populations is not yet understood; disease signs are mainly developed in pups and assessing the health of litters in wild carnivores has big limitations. this study aims to shed light on the virus dynamics among wild carnivores thanks to the analysis of 213 samples collected between 1994 and 2013 in wild ecosystems from spain. we determined the presence of carnivore parvovirus dna by real-time pcr and sequenced the vp2 gen from 22 positive sam ...202031581349
effects of pcb exposure on serum thyroid hormone levels in dogs and cats.polychlorinated biphenyls (pcbs) and their hydroxylated metabolites (oh-pcbs) might disrupt thyroid function. however, there is no clear evidence of pcb exposure disrupting thyroid hormone (th) homeostasis in dogs and cats. the present study conducted in vivo experiments to evaluate the effects of a mixture of 12 pcb congeners (cb18, 28, 70, 77, 99, 101, 118, 138, 153, 180, 187 and 202, each congener 0.5 mg/kg bw, i.p. administration) on serum th levels in male dogs (canis lupus familiaris) and ...201931726548
serological survey of toxoplasma gondii and besnoitia besnoiti in a wildlife conservation area in southern portugal.toxoplasma gondii and besnoitia besnoiti are closely related apicomplexan protozoa. t. gondii is a zoonotic pathogen which may cause serious disease in man and warm-blooded animals, including wild species. b. besnoiti causes bovine besnoitiosis, an emergent disease in europe, which is linked to important production losses. unlike t. gondii, the life cycle of b. besnoiti remains a mystery, since the definitive host has not yet been identified. the aim of this work was to determine the seroprevale ...201631014504
projecting introgression from domestic cats into european wildcats in the swiss jura.hybridization between wild and domesticated organisms is a worldwide conservation issue. in the jura mountains, threatened european wildcats (felis silvestris) have been demographically spreading for approximately the last 50 years, but this recovery is coupled with hybridization with domestic cats (felis catus). here, we project the pattern of future introgression using different spatially explicit scenarios to model the interactions between the two species, including competition and different ...202032908607
endoparasites of wild mammals sheltered in wildlife hospitals and rehabilitation centres in greece.wildlife parasitic diseases represent an important field of investigation as they may have a significant impact on wild animals' health and fitness, and may also have zoonotic implications. this study aimed to investigate the occurrence of endoparasites in wild mammals admitted to wildlife hospitals and rehabilitation centres in greece. sixty-five animals belonging to 17 species and originated from various areas of continental and insular greece were included in the survey. the most numerous ani ...201729326954
mesocarnivore community structure under predator control: unintended patterns in a conservation context.across the mediterranean, conservation programmes often operate concomitantly with hunting interests within game-lands. carnivore guilds lie at the interface between contrasting management goals, being simultaneously fundamental components of ecosystems and targets of predator control to reduce predation on game species. here, we evaluate the composition and spatial structure of a mesocarnivore community in a protected area of southeast portugal, with high economic investment in conservation and ...201930653547
characterization of mesenchymal stem cells derived from adipose tissue of a cougar (puma concolor).adipose derived mesenchymal stem cells (amscs) have been isolated from domestic and wild cats. for wild cats, the isolation of amscs has been reported in the black-footed cats (felis nigripes) and guigna (leopardus guigna). stromal vascular fraction (svf) isolated from cougar adipose tissue have been used to restore elbow functionality in the cougar (puma concolor) but multipotent characteristics of these cells have not been described. the present study describes for the first time the isolation ...202032714450
is angiostrongylosis a realistic threat for domestic cats?three species of angiostrongylus have been found in felids thus far, i.e., angiostrongylus chabaudi, angiostrongylus felineus and angiostrongylus vasorum. angiostrongylus chabaudi lives in the right heart and pulmonary arteries of the definitive natural host, the european wildcat (felis silvestris), and non-patent infections have been reported in domestic cats (felis catus). angiostrongylus felineus, described in the puma yaguarondi (herpailurus yagouaroundi), has never been reported in domestic ...202032351980
attachment bonds between domestic cats and humans.worldwide, domestic cats (felis silvestris catus) outnumber domestic dogs (canis familiaris). despite cats' success in human environments, dog social cognition has received considerably more scientific attention over the last several decades [1-3]. a key aspect of what has been said to make dogs unique is their proclivity for forming attachment bonds, including secure attachments to humans [1,3], which could provide scaffolding for the development of human-like socio-cognitive abilities and cont ...201931550468
all about toxoplasmosis in cats: the last decade.toxoplasma gondii infections are common in humans and animals worldwide. toxoplasmosis continues to be of public health concern. cats (domestic and wild felids) are the most important host in the epidemiology of toxoplasmosis because they are the only species that can excrete the environmentally resistant oocysts in feces. cats can excrete millions of oocysts and a single cat can spread infection to many hosts. the present paper summarizes information on prevalence, persistence of infection, cli ...202032645556
tracking the fate of endogenous retrovirus segregation in wild and domestic cats.endogenous retroviruses (ervs) of domestic cats (erv-dcs) are one of the youngest feline erv groups in domestic cats (felis silvestris catus); some members are replication competent (erv-dc10, erv-dc18, and erv-dc14), produce the antiretroviral soluble factor refrex-1 (erv-dc7 and erv-dc16), or can generate recombinant feline leukemia virus (felv). here, we investigated erv-dc in european wildcats (felis silvestris silvestris) and detected four loci: erv-dc6, erv-dc7, erv-dc14, and erv-dc16. erv ...201931534037
cellular characterization and effects of cryoprotectant solutions on the viability of fibroblasts from three brazilian wild cats.preserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. however, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. considering this, fibroblasts from jaguar (panthera onca), oncilla (leopardus tigrinus), and pampas cat (leopardus colocolo) were subjected to cell characterization and then cryopreservation i ...202033035068
molecular survey of bartonella spp. and haemoplasmas in american minks (neovison vison).the aim of this study was to perform a molecular survey and characterize bartonella spp. and haemotropic mycoplasma (haemoplasmas) in invasive american minks (neovison vison) from southern chile. additionally, we addressed risk factors for positivity in both groups of agents. blood and/or tissue samples from 246 minks were analysed by qpcr targeting the nuog gene for bartonella spp. and conventional (c)pcr for 16s rrna for haemotropic mycoplasma spp. nuog qpcr-positive bartonella spp. samples we ...202032985137
first report of a troglostrongylus brevior case in a domestic cat in turkeyparasitic bronchopneumonia plays an important role, especially in the respiratory diseases of cats. troglostrongylus species have been reported as lung parasites of wild cats; however, in recent years, they have also been found in domestic cats, especially from the mediterranean and balkan countries. in this report, troglostrongylus brevior was found during the examination of lung samples of a tabby cat who was euthanized at the age of 2 years. to the best of our knowledge, this is the first rep ...202032928727
a combined morphological and molecular approach for hair identification to comply with the european ban on dog and cat fur trade.animal furs are encountering more and more the detriment of public opinion, that is increasingly sensitive to animals, their welfare and protection. the feeling of outrage against animal suffering is particularly intense when cats and dogs are involved, since these are the most popular pets in western countries. however, in some asian countries breeding of dogs and cats for the fur industry is a common practice. these furs and their finished garments are often mislabelled in order to be imported ...201931737444
sars-cov-2 natural transmission from human to cat, belgium, march 2020.in march 2020, a severe respiratory syndrome developed in a cat, 1 week after its owner received positive test results for severe acute respiratory syndrome coronavirus 2. viral rna was detected in the cat's nasopharyngeal swab samples and vomitus or feces; immunoglobulin against the virus was found in convalescent-phase serum. human-to-cat transmission is suspected.202032788033
streptobacillus felis, a member of the oropharynx microbiota of the felidae, isolated from a tropical rusty-spotted cat.streptobacillus felis is a fastidious microorganism and a novel member of the potentially zoonotic bacteria causing rat bite fever. since its description, this is the second isolation of s. felis in a diseased member of the felidae. interestingly, the strain from this study was isolated from a zoo held, rusty-spotted cat (prionailurus rubiginosus), with pneumonia, thereby indicating a possible broader host range in feline species. a recent preliminary sampling of domestic cats (felis silvestris ...202032776203
molecular detection of gurltia paralysans by semi-nested pcr in cerebrospinal fluid and serum samples from domestic cats (felis catus).gurltia paralysans is an angio-neurotropic metastrongyloid nematode that infects domestic and wild cats, invading the veins of the subarachnoid space of the spinal cord and mainly causing progressive paralysis of the pelvic limbs. the definitive diagnosis of feline gurltiosis can only be achieved by post-mortem examination that reveals the presence of the nematode in the spinal cord vein vasculature. an early diagnosis with conclusive results is required since laboratory and imaging findings are ...202032660139
intermediate gastropod hosts of major feline cardiopulmonary nematodes in an area of wildcat and domestic cat sympatry in greece.the metastrongyloid nematodes aelurostrongylus abstrusus, troglostrongylus brevior and angiostrongylus chabaudi are cardiopulmonary parasites affecting domestic cats (felis catus) and wildcats (felis silvestris). although knowledge on these nematodes has been improved in the past years, gaps in our knowledge of their distribution and role of gastropods as intermediate hosts in europe still exist. this study reports on the presence of these nematodes and their intermediate hosts in an area in gre ...202032650821
distribution of infectious endogenous retroviruses in mixed-breed and purebred cats.endogenous retroviruses of domestic cats (erv-dcs) are members of the genus gammaretrovirus that infect domestic cats (felis silvestris catus). uniquely, domestic cats harbor replication-competent proviruses such as erv-dc10 (erv-dc18) and erv-dc14 (xenotropic and nonecotropic viruses, respectively). the purpose of this study was to assess invasion by two distinct infectious erv-dcs, erv-dc10 and erv-dc14, in domestic cats. of a total sample of 1646 cats, 568 animals (34.5%) were positive for er ...202031748876
genetic instability of a polydactyl hypopigmented cat with squamous cell carcinoma-a case report.polydactyly, hypopigmentation, and squamous cell carcinoma are common in cats. however, a cat exhibiting all of these conditions has not yet been reported. this study presents the case of a 14- year-old male mexican cat, hypopigmented, with supernumerary fingers, two preaxial and one on each posterior limb, admitted to the clinic with a lesion in the left periocular region. the cat was subjected to a general physical examination, blood, and urine chemistry, as well as a biopsy and genomic instab ...202032528983
evidence for individual differences in behaviour and for behavioural syndromes in adult shelter cats.consistent inter-individual differences in behaviour have been previously reported in adult shelter cats. in this study, we aimed to assess whether repeatable individual differences in behaviours exhibited by shelter cats in different situations were interrelated, forming behavioural syndromes. we tested 31 adult cats in five different behavioural tests, repeated three times each: a struggle test where an experimenter restrained the cat, a separation/confinement test where the cat spent 2 min in ...202032492877
characterization of amino acid substitutions in feline coronavirus 3c-like protease from a cat with feline infectious peritonitis treated with a protease inhibitor.feline infectious peritonitis (fip) is a highly fatal disease caused by a virulent feline coronavirus in domestic and wild cats. we have previously reported the synthesis of potent coronavirus 3c-like protease (3clpro) inhibitors and the efficacy of a protease inhibitor, gc376, in client-owned cats with fip. in this study, we studied the effect of the amino acid changes in 3clpro of feline coronavirus from a feline patient who received antiviral treatment for prolonged duration. we generated rec ...201931585653
illegal trade in wild cats and its link to chinese-led development in central and south america.seizures of hundreds of jaguar heads and canines in central and south america from 2014 to 2018 resulted in worldwide media coverage suggesting that wildlife traffickers are trading jaguar body parts as substitutes for tiger parts to satisfy the demand for traditional asian medicine. we compiled a data set of >1000 seized wild cats (jaguar [panthera onca], puma [puma concolor], and ocelot [leopardus pardalis]) from 19 central and south american countries and china. we ran generalized additive mi ...202032484587
arts in wild felid conservation programmes in poland and in the world.with the exception of the domestic cat, all felid species (felidae) are currently threatened with extinction in their natural habitat. to develop effective and optimal wild cat conservation programmes with assisted reproductive technology (art) it is necessary to combine advances from different disciplines of science, starting from the biology of the species, through research into the population and habitat, assisted reproductive technologies, establishment of gene banks, developing bioinformati ...201931572828
anatomical description of the origin and distribution of the lumbosacral plexus in one puma (puma concolor).wild felids often suffer spinal and limb disorders; however, their nervous system anatomy is poorly studied. herein, the lumbosacral plexus (plexus lumbosacralis) of an adult puma and the motor and sensitive innervation of the pelvic limb is described. we found anatomical similarities to other felids, but also some differences. branches l4-s3 form the lumbosacral plexus (plexus lumbosacralis) in the puma. the femoral nerve (n. femoris) arises from the union of l4-l5, while in other felids, it is ...202032452572
genomic approaches to identify hybrids and estimate admixture times in european wildcat populations.the survival of indigenous european wildcat (felis silvestris silvestris) populations can be locally threatened by introgressive hybridization with free-ranging domestic cats. identifying pure wildcats and investigating the ancestry of admixed individuals becomes thus a conservation priority. we analyzed 63k cat single nucleotide polymorphisms (snps) with multivariate, bayesian and gene-search tools to better evaluate admixture levels between domestic and wild cats collected in europe, timing an ...201931406125
structural and mechanistic studies of the rare myristoylation signal of the feline immunodeficiency virus.all retroviruses encode a gag polyprotein containing an n-terminal matrix domain (ma) that anchors gag to the plasma membrane and recruits envelope glycoproteins to virus assembly sites. membrane binding by the gag protein of hiv-1 and most other lentiviruses is dependent on n-terminal myristoylation of ma by host n-myristoyltransferase enzymes (nmts), which recognize a six-residue "myristoylation signal" with consensus sequence: m1gxxx[st]. for unknown reasons, the feline immunodeficiency virus ...202032442659
coping styles in the domestic cat (felis silvestris catus) and implications for cat welfare.identifying coping styles in cats may lead to improved health and welfare. the aims of this study were to (1) identify individual differences in response to acute confinement, and (2) to assess the predictability of guardian-rated personality traits on behavior. adult cats (n = 55) were singly housed in enriched cages and behavioral observations were recorded for three days. on day 3, familiar and unfamiliar person approach tests were conducted. fecal glucocorticoid metabolites (fgm) were quanti ...201931216726
reproductive hormones monthly variation in free-ranging european wildcats: lack of association with faecal marking.integrating ecophysiological and behavioural discoveries in conservation and management plans is essential to preserve scarce and elusive species such as the european wildcat (felis silvestris). the purpose of this study was to characterise the monthly variation in the steroid reproductive hormone metabolite levels (estradiol, progesterone and testosterone) in this species, and to test its possible association with a monthly pattern of faecal marking. by collecting fresh faecal samples in montes ...202033070401
margay (leopardus wiedii) in the southernmost atlantic forest: density and activity patterns under different levels of anthropogenic disturbance.the margay (leopardus wiedii) is a small neotropical arboreal wild cat. this species is thought to be forest-dependent, although few studies so far have directly evaluated the relationships between spatiotemporal aspects of its ecology and landscape characteristics. the aim of this study was to estimate margay population density and activity patterns in six areas with different habitat types and levels of anthropogenic disturbance in the southernmost atlantic forest of brazil. our working hypoth ...202032374736
black cat bias: prevalence and predictors.there is anecdotal and empirical evidence for black cat bias, the phenomenon where cats (felis silvestris catus) with black coats are viewed more negatively, adopted less often, and euthanized more often than lighter colored cats. despite the anecdotal claims, there is scarce empirical evidence for black cat bias. using evaluations of cat photos, the researchers examined differences in people's attitudes toward black and non-black cats of various colorations on measures of perceived aggression, ...202031033416
identification of selected wild felids using hair morphology and forensically informative nucleotide sequencing (fins): wildlife forensics prospective.we describe the identification of the leopard cat (prionailurus bengalensis), jungle cat (felis chaus) fishing cat (prionailurus viverrinusis), wild cat (felis silvestris) and caracal (caracal caracal) using combined approach of morphological and molecular based analysis. three mitochondrial genes [12s rrna, 16s rrna and cytochrome b (cyt b)] and hair characteristics (cuticle and medulla patterns) were analysed and variation was observed among few selected wild felids. we did not find conclusive ...202032208347
feline adult adipose tissue-derived multipotent stromal cell isolation and differentiation.the cat, as a species, is somewhat new to the field of adult multipotent stromal cells. despite the relative phylogenetic distance between the domestic cat, felis silvestris catus, and humans, they share some similar health challenges like diabetes, kidney disease and asthma. there is a plethora of current investigative efforts focused on adult adipose tissue-derived multipotent stromal cell (asc) therapies to address these and other conditions. given the small size of domestic cats, particular ...201829687390
reproductive biology and biotechnologies in wild felids.conservation strategies in natural habitats as well as in breeding centers are necessary for maintaining and reinforcing viable populations of wild felids. among the fundamental knowledge that is required for conservation breeding, a solid understanding of reproductive biology is critical for improving natural breeding and enhance genetic diversity. additionally, it offers the opportunity to develop assisted reproductive technologies (arts) in threatened and endangered species. conservation bree ...202032102745
pattern of latrine use by domestic cats on dairy farms and the implications for toxoplasma gondii transmission.toxoplasma gondii is the parasite responsible for toxoplasmosis, a highly prevalent zoonosis that affects humans and warm-blooded animals. faeces of infected cats can contain millions of t. gondii oocysts, which remain infectious in the environment for months. sites repeatedly used by cats for defecation ('latrines') are recognised as hotspots of t. gondii soil contamination, but this contamination varies from one latrine to another. to understand this spatial heterogeneity, camera traps were de ...201931476666
clinical investigations and treatment outcome in a european wildcat (felis silvestris silvestris) infected by cardio-pulmonary nematodes.recently, feline cardio-pulmonary nematodes have attracted high scientific interest, as they are increasingly reported from various areas. most of these parasites have similar transmission patterns and/or host reservoirs, thus they may affect domestic and wild felids living in sympatry. in the present study, a case of multiple cardio-pulmonary parasitism in co-infection with other parasites in a european wildcat is presented. the animal, found exhausted, was hospitalised for recovery and parasit ...202032057385
assessment of persistent organic pollutants in hair samples collected from several iranian wild cat species.persistent organic pollutants (pops), including the organochlorine pesticides (ocps), such as dichlorodiphenyltrichloroethane (ddt) and its metabolites, hexachlorobenzene (hcb), α, β and γ-hexachlorocyclohexane (hch) isomers, and the polychlorinated biphenyl (pcb) congeners (iupac nos. 28, 52, 101, 138, 153, and 180), were determined in hair samples collected from 41 iranian wild cats belonging to 8 different species. this is the first report on the presence of selected pops in feline hair from ...202032044571
molecular survey of metastrongyloid lungworms in domestic cats (felis silvestris catus) from romania: a retrospective study (2008-2011).: background: lungworms are recognized as important agents in the pathology of the respiratory system in domestic cats. while aelurostrongylus abstrusus is worldwide known and studied, troglostrongylus brevior has gained the attention of the scientific community only in the last decade. the pathogenicity of this species seems to be higher than a. abstrusus, causing more severe clinical presentations and being potentially fatal, especially in young animals.202031991881
fragmentation and low density as major conservation challenges for the southernmost populations of the european wildcat.knowledge of population dynamics of threatened species in the wild is key to effective conservation actions. however, at present, there are many examples of endangered animals for which their current situation is unknown, and not just in remote areas and less developed countries. we have explored this topic by studying the paradigmatic case of the european wildcat (felis silvestris silvestris), an endangered small carnivore whose status has been subjectively established on the basis of non-syste ...202031990935
hybridization between felis silvestris silvestris and felis silvestris catus in two contrasted environments in france.european wildcat (felis silvestris silvestris) populations are fragmented throughout most of the whole range of the subspecies and may be threatened by hybridization with the domestic cat f.s. catus. the underlying ecological processes promoting hybridization remain largely unknown. in france, wildcats are mainly present in the northeast and signs of their presence in the pyrenees have been recently provided. however, no studies have been carried out in the french pyrenees to assess their exposu ...202031988727
applying genomic data in wildlife monitoring: development guidelines for genotyping degraded samples with reduced single nucleotide polymorphism panels.the genomic era has led to an unprecedented increase in the availability of genome-wide data for a broad range of taxa. wildlife management strives to make use of these vast resources to enable refined genetic assessments that enhance biodiversity conservation. however, as new genomic platforms emerge, problems remain in adapting the usually complex approaches for genotyping of noninvasively collected wildlife samples. here, we provide practical guidelines for the standardized development of red ...202031925943
granulosa cells in three-dimensional culture: a follicle-like structure for domestic cat vitrified oocytes.follicle-like structures are three-dimensional matrices joint with living cells that allow the in vitro development of female gametes in more physiological conditions. they have been shown to be beneficial to fresh oocytes in different species, and in this study, domestic cat (felis silvestris catus) granulosa cells were used to create a functional follicle-like structure aimed at supporting the in vitro maturation of conspecific vitrified oocytes, key players of fertility preservation programme ...202031913547
assessing cross-species transmission of hemoplasmas at the wild-domestic felid interface in chile using genetic and landscape variables analysis.the co-occurrence of domestic cats (felis silvestris catus) and wild felids in rural landscapes can facilitate pathogen transmission. however, in the relatively-isolated regions of southern south america there have been no comprehensive studies to assess disease transmission risks between domestic cats and forest-dwelling wild felids such as guigna (leopardus guigna). we evaluated hemoplasma infection and the possibility of transmission between domestic cats and guignas by comparing spatial and ...201931727935
feline immunodeficiency virus in puma: estimation of force of infection reveals insights into transmission.determining parameters that govern pathogen transmission (such as the force of infection, foi), and pathogen impacts on morbidity and mortality, is exceptionally challenging for wildlife. vital parameters can vary, for example across host populations, between sexes and within an individual's lifetime.feline immunodeficiency virus (fiv) is a lentivirus affecting domestic and wild cat species, forming species-specific viral-host associations. fiv infection is common in populations of puma (puma co ...201931641451
infection by platynosomum illiciens (= p. fastosum) in domestic cats of araguaína, tocantins, northern brazil.platynosomiasis is a hepatopathy caused by platynosomum illiciens(= p. fastosum) (trematoda: dicrocoelidae), which occurs mainly in domestic and wild cats in tropical and subtropical areas. the objective of this study was to verify the occurrence of p. illiciens infection in domestic cats in the city of araguaína, tocantins, brazil, using necropsy and coproparasitological tests. additionally, we aimed to evaluate the use of two different techniques to diagnose p. illiciens infection in domestic ...202031576973
myiasis in domestic cats: a global review.myiasis is an infestation caused by larvae of diptera in humans and other vertebrates. in domestic cats, felis silvestris catus l. (carnivora: felidae), four dipteran families have been reported as agents of obligatory and facultative myiasis: oestridae, calliphoridae, sarcophagidae and muscidae. among agents of obligatory myiasis, the most frequent genus is cuterebra clark (oestridae) and the most frequent species is cochliomyia hominivorax (coquerel) (calliphoridae). among the agents of facult ...201931358036
troglostrongylus brevior: a new parasite for romania.the genus troglostrongylus includes nematodes infecting domestic and wild felids. troglostrongylus brevior was described six decades ago in palestine and subsequently reported in some european countries (italy, spain, greece, bulgaria, and bosnia and herzegovina). as the diagnosis by the first-stage larvae (l1) may be challenging, there is a possibility of confusion with aelurostrongylus abstrusus. hence, the knowledge on the distribution of this neglected feline parasite is still scarce. the pr ...201729208009
molecular detection of arthropod-borne pathogens in eurasian badgers (meles meles) from the united kingdom.arthropod-borne diseases (abd) are of increasing interest in veterinary and public health. eurasian badgers (meles meles) are known to harbor a wide range of pathogens, but information on their role as abd reservoirs and their potential epidemiological relevance is limited. this study aimed to investigate the occurrence of arthropod-borne pathogens, specifically piroplasmids and the bacteria anaplasma phagocytophilum, ehrlichia canis, coxiella burnetii, francisella tularensis and bartonella spp. ...202032155963
anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms.feline immunodeficiency virus (fiv) causes aids-like symptoms in domestic and wild cats. treatment of infected cats has been performed using human anti-hiv drugs, which showed some limitations. this study aimed to determine the anti-fiv potential of some mushrooms.202033132590
can jaguar (panthera onca) ovulate without copulation?threatened of extinction in brazil, the jaguar is the largest predator in latin america, playing an important role in the ecosystem where it is inserted. despite of some important studies in this species, its reproductive physiology needs to be better understood for the development of more effective reproductive biotechnologies. one well studied biological aspect is the occurrence of ovulation following stimulation of the vaginal floor during copulation. this mechanical stimulation is responsibl ...202032092606
the global serological prevalence of toxoplasma gondii in felids during the last five decades (1967-2017): a systematic review and meta-analysis.felids (domestic and wild cats) are important in the epidemiology of the parasite toxoplasma gondii because they are the only hosts that can excrete the environmentally resistant oocysts. we conducted a systematic review and meta-analysis to estimate the global prevalence of t. gondii in species of the family felidae.202032066495
laterality as a tool for assessing breed differences in emotional reactivity in the domestic cat, felis silvestris catus.cat breeds differ enormously in their behavioural disposition, a factor that can impact on the pet-owner relationship, with indirect consequences for animal welfare. this study examined whether lateral bias, in the form of paw preference, can be used as a tool for assessing breed differences in emotional reactivity in the cat. the paw preferences of 4 commonly owned breeds were tested using a food-reaching challenge. cats were more likely to be paw-preferent than ambilateral. maine coons, ragdol ...201931484446
gc × gc-ms-based volatile profiling of male domestic cat urine and the olfactory abilities of cats to discriminate temporal changes and individual differences in urine.domestic cats (felis silvestris catus) are solitary and territorial, and mark their territories by spraying urine, which emits a strong odor produced by volatile organic compounds (vocs). previous studies have focused on identifications of specific vocs, such as 3-mercpto-3-methyl-1-butanol, a cat-specific vocs. however, little is known about how whole volatile profiles of their sprayed urine change over time or how the profiles differ among individuals. this study investigated temporal changes ...201931256322
stable individual differences in vocalisation and motor activity during acute stress in the domestic cat.the behavioural assessment of individual animals in stressful situations should consider measures which are consistent across repeated testing, and therefore truly representative of an individual's behaviour. here we report a study conducted on 40 neutered adult cats (felis silvestris catus) of both sexes, originating from two animal shelters in mexico and hungary. we recorded the responses of the cats to repeated brief confinement trials that mimicked a common situation (confinement in a pet ca ...201931132445
emerging feline vector-borne pathogens in italy.the epidemiology of feline vector-borne pathogens (fevbps) has been less investigated in cats than in dogs. the present study assessed the prevalence of rickettsia spp., babesia spp., cytauxzoon spp. and leishmania infantum infections in cat populations living in central italy, by molecular and serological tools.201931046822
pathogenesis of oral type i feline infectious peritonitis virus (fipv) infection: antibody-dependent enhancement infection of cats with type i fipv via the oral route.feline infectious peritonitis virus (fipv) causes a severe, immune-mediated disease called fip in domestic and wild cats. it is unclear whether fip transmits from cat to cat through the oral route of fipv infection, and the reason for this includes that fip is caused by oral inoculation with some fipv strains (e.g., type ii fipv wsu 79-1146), but is not caused by other fipv (e.g., type i fipv ku-2 strain: fipv-i ku-2). in this study, when cats passively immunized with anti-fipv-i ku-2 antibodies ...201931019150
a comparative survey of microsatellites among wild and domestic cat provides valuable resources for marker development.information on the level and distribution of genetic variation is important for conservation plan of captive population of an endangered species such as tiger and cheetah. we assayed the frequency of microsatellites in the genomic and genic sequences of wild cats (panthera tigris, acinonyx jubatus) and compared it with the domestic cat (felis catus). frequency, relative abundance and density of microsatellites were highest in the domestic cat when compared with wild cats. the frequency of micros ...201930864111
morphology, morphometry, ultrastructure, and mitochondrial activity of jaguar (panthera onca) sperm.the jaguar is categorized as "near threatened". conservation strategies, therefore, are needed which include use of reproductive biotechniques. for implementation of biotechnique use, the reproductive characteristics of the species must be understood, which is currently not the case. this study, therefore, aimed to describe the detailed morphology of jaguar sperm, and to evaluate the sperm mitochondrial activity. five male adults were used. slides stained with rose bengal were used for morphomet ...201930853119
hybridization and introgression during density-dependent range expansion: european wildcats as a case study.interbreeding between historically allopatric species with incomplete reproductive barriers may result when species expand their range. the genetic consequences of such hybridization depend critically on the dynamics of the range expansion. hybridization models during range expansion have been developed but assume dispersal to be independent from neighboring population densities. however, organisms may disperse because they are attracted by conspecifics or because they prefer depopulated areas. ...201930815854
severe verminous pneumonia caused by natural mixed infection with aelurostrongylus abstrusus and angiostrongylus chabaudi in a european wildcat from western balkan area.aelurostrongylus abstrusus (railliet, 1898) and angiostrongylus chabaudi (biocca, 1957) are important cardiopulmonary metastrongyloids in felids. this case report describes, for the first time, a natural and patent mixed infection caused by a. abstrusus and a. chabaudi in a european wildcat (felis silvestris silvestris) from bosnia and herzegovina. most important, this is a rare report of fatal parasitism in wildlife, involving a severe verminous bronchopneumonia and gastrointestinal nematode an ...201930756237
cytauxzoon felis infection in domestic cats, yunnan province, china, 2016.we performed a molecular survey for cytauxzoon felis infection in 311 domestic cats in yunnan province, china, in 2016 and found a prevalence of 21.5%. c. felis infection in domestic and wild cats in other provinces should be investigated to determine parasite prevalence and genetic diversity among cats throughout china.201930666952
antiviral activity of itraconazole against type i feline coronavirus infection.feline coronaviruses (fcovs) are the causative agents of severe systemic disease (feline infectious peritonitis: fip) in domestic and wild cats. fcovs have been classified into serotypes i and ii. type i fcov is the dominant serotype (approximately 70-90%) worldwide. therefore, it is necessary to provide antiviral agents for type i fcov infection. in this study, we demonstrated that itraconazole (icz), practically used for fungal infections in cats, inhibits the type i fcov infection. icz also e ...201930658691
canine distemper virus may affect european wild cat populations in central spain.the main objective of this brief communication is to inform about the exposure to certain pathogens of interest for mesocarnivores in wildcats (felis silvestris silvestris) that inhabit a human-domestic-wild ecotone located in a natural park (serranía de cuenca, central spain). blood and mucosal swabs (nasal, conjunctival and rectal) samples were collected from nine alive animals to detect canine distemper virus (cdv), parvovirus (cpv/fpv), feline leukaemia virus (felv), feline coronavirus (fcov ...201932218716
evaluating metabarcoding to analyse diet composition of species foraging in anthropogenic landscapes using ion torrent and illumina sequencing.dna metabarcoding of faecal samples is being successfully used to study the foraging niche of species. we assessed the ability of two benchtop high-throughput sequencing (hts) platforms, to identify a large taxonomic array of food items from domestic cats felis silvestris catus, including prey and human-related food taxa (pet food and leftovers leaving undetectable solid remains in faeces). scats from a captive feeding trial (n = 41) and from free-ranging individuals (n = 326) were collected and ...201830459313
perception of the delboeuf illusion by the adult domestic cat (felis silvestris catus) in comparison with other mammals.the comparative study of the perception of visual illusions between different species is increasingly recognized as a useful noninvasive tool to better understand visual perception and its underlying mechanisms and evolution. the aim of the present study was to test whether the domestic cat is susceptible to the delboeuf illusion in a manner similar to other mammalian species studied to date. for comparative reasons, we followed the methods used to test other mammals in which the animals were te ...201930394784
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