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migration of newly generated neurons upon ependymally derived radial guide cells in explant cultures of the adult songbird forebrain.the adult songbird forebrain undergoes neuronal production throughout adulthood, with the production of new neurons in discrete regions of the neostriatal ventricular zone. upon mitogenesis, these new neurons migrate into the subjacent brain parenchyma along radially directed guide fibers. in long-term ventricular zone explant cultures, derived from the higher vocal center of the adult canary, newly migratory neurons were found to associate preferentially with a characteristic substrate cell typ ...19938225557
[hemoglobinopathies and thalassemias in the hindu settlement established in the canary islands]. 19938235954
yolk is a source of maternal testosterone for developing birds.the sex steroid hormones that affect development in birds have been thought to be produced exclusively by the embryo or neonate. i used radioimmunoassay to measure the amounts of androstenedione, 5 alpha-dihydrotestosterone, testosterone, 17 beta-estradiol, and corticosterone in the yolk of freshly laid canary (serinus canaria) and zebra finch (poephila guttata) eggs. testosterone was found in both canary and zebra finch eggs, but its contents were much higher in the former than in the latter. t ...19938265571
hb porto alegre or alpha 2 beta 29(a6)ser->cys in unrelated families of the canary islands. 19938294204
antibiotic and cytotoxic activity of brominated compounds from the marine sponge verongia aerophoba.analysis of the marine sponge verongia aerophoba from the canary islands afforded eight brominated secondary metabolites including the small molecular weight compounds aeroplysinin-1 (5) and the dienone (7) which were previously shown to arise by enzymatically catalyzed degradation of aerophobin-2 (4) and isofistularin-3 (1) following breakdown of the cellular compartmentation of the sponge. all compounds were identified from their nmr and mass spectra. aeroplysinin-1 as well as dienone which ar ...19938297426
seasonal and tissue-specific regulation of canary androgen receptor messenger ribonucleic acid.natural seasonal fluctuations in androgen levels appear to cause changes in physiology and reproductive behavior, such as singing, in canaries. little is known, however, about the cellular mechanisms underlying these changes. because androgens act principally through nuclear receptors in other species, we have isolated and sequenced a cdna likely to encode the canary androgen receptor and used this cdna to examine the regulation of ar mrna levels in the testis, kidney, and liver of the canary. t ...19948299561
[perceived morbidity and the use of medical consultations in telde (grand canary)].to find the extent of perceived morbidity and the population's main reasons for seeking medical care.19938329549
[intake of pollutants (heavy metals) via total diet at the national level as a whole and in autonomous communities: a new approach].as an answer to the request of information on the real intake of pollutants, both who and fao recommend its study through the study of total diet. following their criteria we have determined the contents of arsenic, cadmium, mercury and lead in the total mean spanish diet and in the seventeen autonomous regions, comparing them with the acceptable daily intakes (adi). at the same time it is studied the contribution of the different groups of nutrients to the total pollutant charge. there is no de ...19938341819
the gastrointestinal tract. the canary of the body? 19938355390
direct evidence for loss and replacement of projection neurons in adult canary brain.normally occurring projection neuron loss and replacement were quantified over a 6 month period in the pathway from the high vocal center (hvc) to the robust nucleus of the archistriatum (ra) in adult male canaries. fluorescent latex microspheres were injected into ra in april--a procedure resulting in long-term retrograde labeling of ra-projecting hvc neurons. labeled cell densities were then obtained 4 and 20 d later in april and 195 d later in october. we found that 41-49% of the ra-projectin ...19938385206
implication of central neurohypophyseal hormone receptor-mediated action in timing of reproductive events: evidence from novel observations on the effect of a vasotocin analogue on singing behaviour of the canary.a highly discrete distribution of neurohypophyseal hormone receptors was discovered in the mammalian and avian brain. these receptors are heterogeneous. in rat brain oxytocin (ot) and v1a receptors can be distinguished which bind ot with an order of magnitude difference in affinity and which are located in discrete sites of the limbic-midbrain circuitry. in the brain of the canary low and high affinity vasotocin (vt) sites were identified; the latter putative vt receptors were found exclusively ...19938390083
estrogen receptors in the avian brain: survey reveals general distribution and forebrain areas unique to songbirds.estrogens play an important role in the control and differentiation of species-typical behavior and in endocrine homeostasis of birds, but the distribution and evolution of cells that contain estrogen receptors in the avian brain are poorly understood. this study therefore surveys 26 species in the avian orders anseriformes (1 species), galliformes (2), columbiformes (3), psittaciformes (1), apodiformes (2), and passeriformes (3 suboscines, 14 oscines). indirect immunocytochemistry with the estr ...19938432903
a frequent tyrosinase gene mutation associated with type i-a (tyrosinase-negative) oculocutaneous albinism in puerto rico.we have determined the mutations in the tyrosinase gene from 12 unrelated puerto rican individuals who have type i-a (tyrosinase-negative) oculocutaneous albinism (oca). all but one individual are of hispanic descent. nine individuals were homozygous for a missense mutation (g47d) in exon i at codon 47. two individuals were heterozygous for the g47d mutation, with one having a missense mutation at codon 373 (t373k) in the homologous allele and the other having an undetermined mutation in the hom ...19938434585
toxin detection using a tyrosinase-coupled oxygen electrode.an enzyme-based "electrochemical canary" is described for the detection of cyanide. the sensing system imitates cyanide's site of toxicity in the mitochondria. the terminal sequence of electron transfer in aerobic respiration is mimicked by mediator coupling of tyrosinase catalysis to an electro-chemical system. an enzyme-coupled oxygen electrode is created which is sensitive to selective poisoning. biocatalytic reduction of oxygen is promoted by electrochemically supplying tyrosinase with elect ...19938439010
cystic fibrosis in spain: high frequency of mutation g542x in the mediterranean coastal area.we have determined the frequency of deletion delta f508 and mutation g542x, a nonsense mutation in exon 11 of the cystic fibrosis (cf) gene, in a sample of 400 spanish cf families. mutation g542x represents 8% of the total number of cf mutations in spain, making it the second most common mutation after the delta f508 deletion, which accounts for 48% of cf chromosomes. g542x has a higher frequency in the mediterranean coastal area (14%) and in the canary islands (25%). about 70% of g542x chromoso ...19938454290
effect of heliotherapy on skin and joint symptoms in psoriasis: a 6-month follow-up study.the effect of heliotherapy on psoriasis skin lesions and arthritis was studied in a trial comprising 4 weeks of therapy in the canary islands and a 6-month follow-up period. a total of 373 patients participated in the heliotherapy and 361 patients completed the follow-up period. the severity of skin lesions was evaluated using a psoriasis severity index (psi), and that of the arthropathy by using an arthritis index (ai). during heliotherapy, the psi decreased significantly from the initial media ...19938457451
neurogenesis in adult canary telencephalon is independent of gonadal hormone levels.neurons generated in adulthood are found throughout the canary telencephalon. we are interested in the factors that control the rate of proliferation of stem cells that give rise to these new neurons. the rate of incorporation of newly generated neurons into vocal-control regions varies seasonally. this difference could reflect a higher rate of neurogenesis, a lower rate of cell death, or an altered migration. we examined the incidence of thymidine-labeled cells in the telencephalic ventricular ...19938478689
geographical variations in the prevalence of hiv infection among drug users receiving ambulatory treatment in spain.to identify the factors responsible for the regional differences in hiv-positive serostatus among drug users in spain receiving outpatient treatment, the 17 autonomous regions into which spain is divided were classified as high (mean 52%) or low prevalence groups (mean 34%) depending on the prevalence of seropositivity. in regions where the prevalence of positive serostatus was high, unemployment was more markedly associated with hiv infection than in low prevalence regions, while other potentia ...19938505189
[prevalence of ischemic cardiopathy risk factors on the island of lanzarote].as a fruit of complex interaction of factors, coronary atherosclerosis constitutes the first cause of death in the developed countries.19938510475
cloning, sequencing and expression in escherichia coli of pha a 1 and four isoforms of pha a 5, the major allergens of canary grass pollen.the pollen of canary grass, which was introduced as a pasture grass from europe, is a major allergen source in the external environment of southern australia. this study was performed to characterize the major recombinant allergens of canary grass pollen. it is anticipated that recombinant allergens may be useful in diagnosis and immunotherapy of grass pollen induced allergies.19958564724
occurrence of salmonella in cold-blooded animals in gran canaria, canary islands, spain.the occurrence of salmonella in endemic and subendemic species of lizard and frog of gran canaria, gallotia stehlini and rana perezi, as well as captive reptiles from other regions of the world was investigated. the occurrence of salmonella was statistically higher in endemic and subendemic species than in captive animals (p < 0.001). seventy strains of salmonella were isolated. s. berta and s. gran canaria were the most frequently isolated serotypes. the study of salmonella in gall-bladder cont ...19958572675
thyroxine reduces the production rate of brdu-labeled cells in the ventricular zone of the adult canary brain.when the canary reaches adulthood, neuron production continues in the lateral ventricular zone (vz) of the forebrain. the effect of thyroxine injection on the cell production rate in the vz during adulthood was studied immunohistochemically using bromodeoxyuridine (brdu). the rate of occurrence of brdu-labeled cells was reduced by thyroxine injection (1 mg/body). hyperthyroidism usually promotes increased mitotic activity, but in the present study this was not the case. the decrease in the numbe ...19958592639
phylogeny of the genus hegeter (tenebrionidae, coleoptera) and its colonization of the canary islands deduced from cytochrome oxidase i mitochondrial dna sequences.the genus hegeter comprises 23 species of darkling beetles (tenebrionidae) endemic to the macaronesian archipelagos, with 21 of them exclusive to the canary islands. we have sequenced 438 bp of the mitochondrial cytochrome oxidase i gene in 17 species (24 taxa) of canarian hegeter. estimates of nucleotide composition, transition/transversion ratios and nucleotide change frequencies are very similar to those found in another tenebrionid canarian genus pimelia, indicating that similar molecular me ...19968626224
improved gastric tonometry for monitoring tissue perfusion: the canary sings louder. 19968659716
apolipoprotein e polymorphism influence on lipids, apolipoproteins and lp(a) in a spanish population underexpressing apo e4.the apolipoprotein e (apo e) polymorphism in a spanish working population of tenerife (canary islands, spain) was analyzed. the distribution of apo e alleles (epsilon 3, 0.850; epsilon 2, 0.075; epsilon 4, 0.075) and phenotypes (e3/3, 72.6%; e3/4, 13%; e3/2, 11.5%; e4/4, 0.8%; e2/2, 1.5%; e4/2, 0.5%) was significantly different from those of a combined caucasian population owing to a lower frequency of apo e4. we have also investigated the effect of apo e polymorphism on serum levels of choleste ...19968678918
candida glabrata: in vitro susceptibility of 84 isolates to eight antifungal agents.the in vitro susceptibility of 84 isolates of candida glabrata from patients treated at the university hospital of the canary islands to eight antifungal agents (amphotericin b, itraconazole, fluconazole, miconazole, clotrimazole, tioconazole and econazole) has been studied using the broth dilution micro-method. among the eight antifungal agents tested, the smaller geometric mean corresponded to tioconazole, econazole, clotrimazole and miconazole. in contrast with fluconazole, a greater geometri ...19968697885
canary-pox (alvac) virus-mediated cytokine gene therapy induces tumor specific and non-specific immunity against mouse prostate tumor. 19968701820
daily surface ozone maximum concentrations at taliarte, canary islands.two different methods for using air quality data to estimate daily maximum concentrations along a year of surface ozone concentration in a new measurement station in canary islands are described. it is usually assumed that sequences of pollutant concentration show stationary behaviour. in this paper, it is shown that the application of such procedure could be useful to estimate probabilities of maximum concentration of surface ozone. nevertheless, this method does not take into account the time ...19958712581
mycobacterium genavense infection in canaries.a case of mycobacteriosis in a collection of canaries (serinus canarius) is described. the affected birds showed nonspecific clinical signs and macroscopic lesions (slight splenomegaly). histologic lesions found in liver, spleen, lungs, and kidneys consisted of noncaseous nodules containing accumulations of large macrophages that showed a highly vacuolated cytoplasm in which numerous acid-fast organisms were detected. attempts to isolate and culture the organisms using löwenstein-jensen and cole ...19968713044
anatomical basis of a congenital hearing impairment: basilar papilla dysplasia in the belgian waterslager canary.recent investigations into the anatomy of the inner ear of belgian waterslager canaries (bwc) have demonstrated myriad malformations associated with dysgenesis of the pars inferior of the otocyst. in those studies, the surface anatomy of bwc's basilar papilla and sacculus was examined utilizing scanning electron microscopy. in the present investigation, we utilized both light microscopy and transmission electron microscopy to describe the cross sectional anatomical pathology of the bwc's basilar ...19968727001
sub-saharan influence on the canary islands population deduced from g6pd gene sequence analysis.in a screening of glucose-6-phosphate dehydrogenase (g6pd) a variants in the canary islands and northwest african populations by electrophoresis and posterior gene sequencing, the common a+ 376g and a- 202a/376g and the rare a- 376g/968c mutations were found. in addition, three new silent c-->t transitions have been detected at nucleotides 759 (exon 7), 1338 (exon 11), and 1573 (exon 13). canary island and north african samples share sub-saharan haplotypes with equatorial guineans. the slave tra ...19968754258
a common origin for woody sonchus and five related genera in the macaronesian islands: molecular evidence for extensive radiation.woody sonchus and five related genera (babcockia, taeckholmia, sventenia, lactucosonchus, and prenanthes) of the macaronesian islands have been regarded as an outstanding example of adaptive radiation in angiosperms. internal transcribed spacer region of the nuclear rdna (its) sequences were used to demonstrate that, despite the extensive morphological and ecological diversity of the plants, the entire alliance in insular macaronesia has a common origin. the sequence data place lactucosonchus as ...19968755546
maternal testosterone in the avian egg enhances postnatal growth.the eggs of the canary (serinus canaria) contain variable doses of maternal testosterone. the reported experiments investigated whether testosterone influences nestling growth and how this interacts with differences of the growth of nest mates that are caused by asynchronous hatching. injections of testosterone into the yolk of unincubated eggs enhanced the growth after hatching compared to nestlings that had hatched simultaneously from control eggs. these differences were established within 22 ...19968759148
the king solomon lectures in neuroethology. a white canary on mount acropolis. 19968765555
[continuous proliferation of supporting cells and indications for hair cell differentiation in the inner ear of adult song birds with genetic cochlear hearing loss].our previous investigations demonstrated that the belgian waterslagers (bws) canary (serinus canarius) was affected by an inherited sensorineural hearing loss. compared to normal canaries of others strains, hair cell numbers in these birds were reduced on average by 30%. since other birds are able to replace similar hair cell numbers after cochlear trauma, we investigated if bws have the potential for supporting cell proliferation with subsequent hair cell differentiation or if they lack the rep ...19968767126
an epornitic of avian pox in houbara bustards (chlamydotis undulata macqueenii).an epornitic of avian pox occurred in a flock of 123 houbara bustards (chlamydotis undulata macqueenii) received at the sulman falcon hospital in the state of bahrain in february 1993. birds displayed conjunctivitis, excessive lacrimation and papilloma-like growths forming amorphous clusters on the third eyelid and on the conjunctiva. examination of eyelid samples under transmission electron microscopy revealed pox virus particles displaying the classical morphology of vaccinia-avipox virions. t ...19968779810
polycyclic aromatic hydrocarbons and n-alkanes in osilinus attratus from the coast of tenerife (canary islands). 19968791557
human enzyme polymorphism in the canary islands. vii. g6pd seattle in canarians and north african berbers.sequence analysis of the glucose-6-phosphate dehydrogenase (g6pd) variant gc, characterized by slower electrophoretic mobility than g6pd-b, in 14 canarian and 2 berber males, has revealed that all of them share the g-->c mutation at nucleotide 844 in exon 8 leading to an asp-->his substitution at amino acid 282 known as g6pd seattle. no additional differences have been detected among them nor with the common haplotype previously found for this variant.19968807321
phylogenetic relationships of the canary islands endemic lizard genus gallotia (sauria: lacertidae), inferred from mitochondrial dna sequences.phylogenetic relationships among species and subspecies of the canary island endemic lizard genus gallotia are inferred based on nucleotide sequences of fragments of 12s ribosomal rna and cytochrome b mitochondrial genes. the four morphologically established species have also been recognized at the molecular level. relative affinities among species follow an eastern-western geographic transect. the nearly extinct species gallotia simonyi from the most western island of el hierro is closely relat ...19968812306
phlebotomus sergenti parrot, 1917: morphological and isoenzymatic comparisons of two natural populations from tenerife (canary islands, spain) and crete (greece).samples of two phlebotomus sergenti natural populations from san juan (tenerife), representing the western edge of the distribution area of this species, and axos (crete) were collected. the morphological comparison showed marked differences in the lengths of parts of the male genitalia, female pharynx, and spermathecae. the isoenzyme study revealed characteristic monomorphic phenotypes for glucose phosphate isomerase, hexokinase, and phosphoglucomutase in the canarian specimens as compared with ...19968825445
canary finches (serinus canaria) as an avian infection model for lyme borreliosis.to investigate if passerine birds can be used as an avian model for lyme borreliosis, the borrelia burgdorferi sensu stricto strain sh2-82 was inoculated subcutaneously on canary finches (serinus canaria). spirochaetes could be detected in the blood by direct microscopy and an immunofluorescence assay (ifa) up to 2 weeks after the inoculation. dna amplification (pcr) revealed b. burgdorferi dna in the serum for up to 3 weeks, in the faeces up to 4 weeks, and from several internal organs at autop ...19968831827
genetic relationship between the canary islanders and their african and spanish ancestors inferred from mitochondrial dna sequences.nucleotide sequences of the hypervariable segment i of the control region of the mtdna were determined in 101 individuals: 54 canary islanders, 18 north african berbers, 18 spanish mainlanders and 11 sub-saharan guineans. in spite of the fact that only members of the fang tribe were analysed, nucleotide diversity in guineans (theta x 100 = 2.33) is one of the highest found in african populations. estimates of genetic contribution to the canarians from their putative parental populations based on ...19968865992
island colonization and evolution of the insular woody habit in echium l. (boraginaceae).numerous island-inhabiting species of predominantly herbaceous angiosperm genera are woody shrubs or trees. such "insular woodiness" is strongly manifested in the genus echium, in which the continental species of circummediterranean distribution are herbaceous, whereas endemic species of islands along the atlantic coast of north africa are woody perennial shrubs. the history of 37 echium species was traced with 70 kb of noncoding dna determined from both chloroplast and nuclear genomes. in all, ...19968876207
environment modifies the testosterone levels of a female bird and its eggs.past studies have shown that the yolk of the canary (serinus canaria) egg contains maternal testosterone, that its concentrations increase in the subsequently formed eggs of a clutch, and that testosterone influences development. the present study investigated 1) if the testosterone levels vary in the female during yolk formation; 2) how such putative variations may be related to the concentrations of maternal testosterone in the yolk; and 3) if environmental factors, such as day length, can mod ...19968900078
inhibitory properties coexist with plasticity in the song system of the canary.the expression of neuronal growth regulating factors (ni 35/250, gap-43) were studied immunohistochemically in the plastic telencephalic song system and in the cerebellum of the canary. in the myelinated song nuclei (hvc and ra) growth promoting and inhibiting molecules could be detected, whereas in the white matter tracts of the cerebellum only the neurite growth inhibitor could be detected. the morphodynamic effects of these molecules were assessed by in vitro assays on cryosections. neurites ...19968905681
genetic heterogeneity in hispanic families with autosomal dominant juvenile glaucoma.a gene for autosomal dominant, juvenile-onset, primary open angle glaucoma (glcia) has been previously mapped to 1q21-31 in several caucasian pedigrees. we studied two hispanic families with this disease to determine if their disease genes also map to this region. individuals were considered as being affected if they had 1op > 30 mmhg (without treatment) and glaucomatous optic nerve damage or visual field defects. persons older than 40 years with intraocular pressures < or = 21 mmhg and no evide ...19968905848
host range, survival in dead hosts, cryopreservation, periodicity and morphology of plasmodium durae herman in experimental infections.in experimental infections, fowl, duck, guineafowl and canary were refractory to plasmodium durae, which in japanese quail produced low and transient infections, but a high and long-lasting parasitaemia in a lady amherst pheasant. heart, blood and brain of dead hosts injected into turkeys, allowed the recovery and further passaging of the live parasite. this technique could be useful for the recovery of malaria parasites from suspect postmortem material. intravenous infection produced parasitaem ...19968917860
skeletal muscle ultrastructural pathology in serinus canarius infected with plasmodium cathemerium.serinus canarius infected with plasmodium cathemerium was used as an animal model in order to study the skeletal muscle compromise in malaria. pectoral muscle biopsies were obtained from 7 infected female birds. the transmission electron microscopic study showed alterations of contractile and sarcotubular systems, mitochondrial abnormalities, lysosomal proliferation and nuclear pyknosis. the sarcolemma looked disrupted and separated from the necrotic fibres. capillary abnormalities included endo ...19968929630
house dust mites and der p i in tenerife (canary islands, spain): the relative importance of other non dermatophagoides spp mites.house dust mites constitute the major etiologic factor causing sensitization and asthma its geographical distribution map is quite well known, although it is not yet complete. home environments world-wide show mites that mainly belong to the piroglyphidae family, the d. pteronyssinus specie being the predominant one. however others have also been considered of interest.19968939268
blockade of nmda receptors in the anterior forebrain impairs sensory acquisition in the zebra finch (poephila guttata).juvenile zebra finches (poephila guttata) learn song in two stages: during sensory acquisition, they memorize the song of an adult tutor, and during sensorimotor learning, they alter their vocalizations to match the stored song model. like many other forms of neural plasticity and memory formation, vocal learning in zebra finches is impaired by pharmacological blockade of nmda receptors, but the relevant nmda receptors have not yet been localized. during song development, one neural region that ...19968946423
clinical presentation of acute q fever in lanzarote (canary islands): a 2-year prospective study.the clinical manifestations of acute q fever may differ markedly from country to country. in this regard, fever and hepatitis seem to be the dominant clinical features of acute coxiella burnetii infection in lanzarote, canary islands. a possible interaction between environmental factors and some strains of c. burnetii could explain the different clinical presentations of acute q fever.19968953689
genetic study in panaquire, a venezuelan population.panaquire is a population in venezuela founded in 1733 by spaniards from the canary islands. in order to define the genetic characteristics of this population the following loci were studied: abo, rh (cc, d, ee), fy, k, jk, hb, esd, acp1, hp, tf, cp and alb. its sociohistorical features were also investigated. historic censuses reveal that ethnically blacks were the most important group in this village during the year 1800 (76%), followed by amerindians (9%), mulattoes (5%) and whites (7%). the ...19968956028
seroprevalence of toxocariasis in children and adults in madrid and tenerife, spain.a study on the seroprevalence of toxocariasis, using elisa with toxocara larval excretory-secretory antigens, was carried out on human populations in two regions of spain. sera from a population of 195 children from madrid and 143 children from santa cruz de tenerife (canary isles), showed a prevalence of 0% and 4.2% respectively. sera from a population of 272 adults from madrid and 803 adults from santa cruz de tenerife showed a prevalence of 3.6% and 17.4%. reasons for these differences in the ...19968960205
mitochondrial dna sequence variation and phylogeography of pimelia darkling beetles on the island of tenerife (canary islands).four morphological taxa of the beetle genus pimelia (coleoptera, tenebrionidae) are known to exist on the atlantic island of tenerife. we have obtained dna sequences for 61 individuals from these taxa across the island for a 200 bp long fragment of the mitochondrial coi gene. in addition, a restriction site polymorphism in the nuclear rrna its-1 sequence was identified and screened in a sample of these individuals using the enzyme kpn2i. the results were analysed using approaches which allow inf ...19968972079
evaluation of osilinus attratus as a bioindicator organism to monitor oil pollution in the canary islands.concentrations of n-alkanes and pahs in the visceral mass of the marine winkle osilinus attratus taken from three locations on the southeast coast of tenerife (canary islands, spain) over a two-year period, were evaluated at monthly intervals in order to assess if this small marine winkle may be used as bioindicador organism. factor analysis and cluster analysis were applied in order to determine if it was possible to relate sampling areas to potential sources of petroleum contamination.19968975815
cross-protection test of an avian poxvirus isolated from houbara bustards.an avian poxvirus was isolated previously from the houbara bustard (chlamydotis undulata). we carried out a cross-protection test on 66 captive-bred canaries. thirty-five canaries were vaccinated with a commercial canary poxvirus (cp) vaccine. three weeks later all 66 birds were assigned randomly to six different groups: group ia (n = 14) was vaccinated and challenged with houbara bustard poxvirus (hp) strain; group ib (n = 13) was vaccinated and challenged with a cp strain; group ic (n = 7) was ...19968980803
cloning and expression pattern of hor v 9, the group 9 pollen isoallergen from barley.in this study we report the cloning, sequence, and characterization of hor v 9 allergen cdnas from barley (hordeum vulgare) pollen. structural homologues of kentucky bluegrass (poa pratensis) group 9 pollen allergens were identified in a cdna library of barley pollen expressed mrnas. the hor v 9 cdna clone (hvp9742) contained an open reading frame encoding 313 amino acids which included a putative 27-residue signal peptide and one asparagine sequon for glycosylation. the mrna corresponding to cl ...19968982067
evidence for supporting cell proliferation and hair cell differentiation in the basilar papilla of adult belgian waterslager canaries (serinus canarius).we used the bromodeoxyuridine technique to study the proliferative activity in the basilar papilla of normal and belgian waterslager canaries with and without preceding sound trauma. without sound trauma, there were, on average, six supporting cell divisions per day in the basilar papilla of waterslager canaries. this rate of supporting cell proliferation corresponds well with estimates of the rate of hair cell differentiation derived from counts of immature-appearing hair cells obtained by usin ...19978986868
[clinical characteristics and epidemiologic study of a listeriosis outbreak in grand canary].background and methods: human infections caused by listeria monocytogenes often present as sporadic cases without any epidemiological relationship among them; however they also appear as outbreaks that are usually detected by an increase in the number of cases diagnosed by hospitals of the geographic area. between december 1991 and may 1993, twenty four cases of listeriosis were detected in three hospitals of las palmas de gran canaria; and they were classified as an outbreak. our report describ ...19968991435
quantity of k, ca, na, mg, fe, cu, pb, zn and ashes in doc tacoronte-acentejo (canary islands, spain) musts and wines.this study determines ash, k, ca, na, mg, fe, cu, pb, and zn content in musts and wines of the doc tacoronte-acentejo (tenerife, canary islands) from two consecutive harvests. samples were treated with hno3 and h2o2. na and k were determined by flame photometry and the remaining metals by air/acetilene flame atomic absorption spectrophotometry. significant differences were observed in na, ca and mg contents between both harvests, possibly due to climate conditions.19969002177
historical background of aeropalynology in the canary islands.this paper forms part of a doctorate thesis, currently being completed at the allergy and immunology section of the ntra. sra. de la candelaria hospital, tenerife, regarding the epidemiology of pollens we would like to illustrate that this island and its provinces are similar to a miniature continent, where pollens from the most important allergenic families present in the national territory of the iberian peninsula can be found. we have a very high incidence of patients allergic to grass pollen ...19969010563
heeding warnings from the canary, the whale, and the inuit.there is a tension between traditional and modern definitions of reproductive risk and normalcy. this excerpt describes that tension as it plays out among the inuit of northern canada from the perspective of a community midwife who has worked with the inuit. she presents an analytical framework which classifies and illuminates the types of logic that compete in most birth settings around the world-a framework useful for showing how some types of logic can be supervalued while others, such as cul ...19969016068
intracerebral sex differences in the vasotocin system in birds: possible implication in behavioral and autonomic functions.the brain vasotocinergic system demonstrates clear sexual dimorphism in birds investigated so far. this paper examines the evidence obtained in studies on gallinaceous (domestic fowl, japanese quail) and passerine (canary, junco, zebra finch) birds. vasotocin (vt)-immunoreactive parvocellular neurons are present in the nucleus of stria terminalis of males, but they are less abundant or absent in the corresponding structure of females. a similar difference has been observed in the dorsal paravent ...19969047289
the ontogeny of the canary hvc revealed by the expression of androgen and oestrogen receptors.the vocal control nucleus hvc (nucleus hyperstriatalis ventrale, pars caudale) of the canary (serinus canaria) is a model in which to study the relationship between anatomical plasticity and vocal developmental learning. much of the structural plasticity of the hvc is sensitive to the action of androgenic and oestrogenic gonadal hormones that affect the brain by binding to androgen receptors (ar) and oestrogen receptors (er). here we report developmental changes of ar and er expression in the hv ...19969051802
a serologic study of human toxocariasis in the canary islands (spain): environmental influences.the presence of anti-toxocara antibodies in the human population of the canarian archipelago was studied by means of an indirect enzyme-linked immunosorbent assay with excretory/secretory antigen of infective-stage larva. a total of 14,074 were analyzed, resulting in the following distribution and prevalence by location: southern tenerife 6,435 (2.5% positive), northern tenerife 1,710 (6.7% positive), la palma 1,214 (4.5% positive), la gomera 264 (6.8% positive), el hierro 204 (3.9% positive), g ...19979063372
[income, percent of women living in rural areas, parity, and breast cancer mortality in spain, 1975-1991].the aim of the present study was to analyze breast cancer mortality by provinces in spain during the period 1975-91, and to assess the relationship with the geographical distribution of income level, percent of women living in rural areas and average parity of women in each province.19979064415
"shattered illusions--the thiepval barracks bombing, 7 october 1996".the provisional ira's ceasefire ended abruptly with the canary wharf bombing in london in february 1996. hopes that violence would not return to northern ireland were shattered by the explosion of two car bombs in the headquarters of the british army in ulster in october 1996. the second bomb specifically targeted medical staff treating casualties from the first explosion. this article describes the medical aspects of this incident, including the extensive use of critical incident stress debrief ...19979089545
genetic study of the population of tenerife (canary islands, spain): protein markers and review of classical polymorphisms.data on six protein polymorphisms (19 alleles) from the human population of tenerife are presented and discussed along with other classical markers in relation to the origin of the canarians. genetic influences from three population groups were considered: the iberians, and the berbers and non-berbers (arabs) from north africa. the systems examined show the tenerife population lies within the limits of variation described for various iberian groups, with a slight tendency towards the characteris ...19979098503
population genetic structure and colonization sequence of drosophila subobscura in the canaries and madeira atlantic islands as inferred by autosomal, sex-linked and mtdna traits.the genetic structure in atlantic islands and continental populations of drosophila subobscura has been studied using autosomal and sex-linked allozymes and mitochondrial dna (mtdna) haplotypes. from the data it is deduced that whereas the canary islands have long been isolated, the neighboring island of madeira has been subjected to continuous migration from the mainland. in addition, sex-linked allozymes and mtdna data show a large divergence between the geologically younger western islands of ...19979099006
reafferent thalamo- "cortical" loops in the song system of oscine songbirds.songbirds have a complex vocal repertoire, much of which is learned by imitation. the vocal motor system of songbirds includes a set of telencephalic pathways dedicated to the acquisition and production of learned song. the main vocal motor pathway goes from the high vocal center (hvc) to the robust nucleus of the archistriatum (ra), which in turn innervates mesencephalic and medullary nuclei involved in vocalization. we used neural tract tracers (biotinylated dextran amines, fluorescein- and rh ...19979100137
[an anthropometric equation for the determination of the percentage of body fat in young men from the canarian population].yet there are no anthropometric equations specific for the canary islands adult population. the purpose of this study was to develop an anthropometric equation specific for the canary islands adult male population, using dual-energy x-ray absorptiometry (dexa) as the reference method.19979102485
[a review of q fever in the canary islands]. 19979102680
canine myiasis by sheep bot fly (diptera:oestridae).we present a case of canine infestation by 3rd instars of oestrus ovis (l.) in a 10-yr-old cross-bred dog (collie x german shepherd) from fuerteventura, canary islands, spain. this report confirms that this fly can develop in dogs.19979103770
motor-driven gene expression.there is increased neuronal firing in the high vocal center (a motor nucleus) and other song nuclei of canaries, serinus canaria, and zebra finches, taeniopygia guttata, whenever these songbirds sing or hear song. these observations suggested that song perception involved sensory and motor pathways. we now show that the act of singing, but not hearing song, induces a rapid and striking increase (up to 60-fold) in expression of the transcriptional regulator zenk in the high vocal center and other ...19979108111
european collaboration identifies an outbreak of escherichia coli q157 infection in visitors to fuerteventura, canary islands. 19979136447
the natural history of apis-specific igg and igg4 in beekeepers.most published studies on changes of specific igg or its subclasses as a response to stimuli by allergens have been performed on patients under immunotherapy. there are few reports analysing the response to immunoglobulins in patients exposed to allergens in their natural habitats.19979146935
genetic differentiation of continental and island populations of bombus terrestris (hymenoptera: apidae) in europe.ten microsatellite loci and a partial sequence of the coii mitochondrial gene were used to investigate genetic differentiation in b. terrestris, a bumble bee of interest for its high-value crop pollination. the analysis included eight populations from the european continent, five from mediterranean islands (six subspecies altogether) and one from tenerife (initially described as a colour form of b. terrestris but recently considered as a separate species, b. canariensis). eight of the 10 microsa ...19969147693
left syringeal dominance in testosterone-treated female canaries.male canaries (serinus canaria) produce most of their song syllable types on the left side of the syrinx, the two-sided vocal organ. female canaries treated with testosterone propionate begin to sing in a male fashion, a behavior seldom seen in untreated females. to learn whether syringeal dominance occurs in testosterone-treated female canaries, we deactivated either the right or the left side of the syrinx by severing the tracheosyringeal nerve on that side. recordings of the birds' songs were ...19979159762
equine babesiosis associated with strenuous exercise: clinical and pathological studies in jordan.clinical, haematological and pathological studies were undertaken in jordan in a stud of 103 racing horses clinically suffering from babesiosis and apparently healthy animals. out of 47 horses which participated in strenuous exercise, three mares showed sudden onset of immobility and reluctance to move and two mares died. clinical examination revealed that these five horses (group 1) had fever, anorexia, weakness and severe icterus and, in two mares, haemoglobinuria. haematological examination r ...19979187024
hormonema schizolunatum, a new species of dothideaceous black yeasts from phyllosphere.two black yeast isolates from plants from the canary islands (spain) are described and illustrated. absence of woronin bodies at simple septal pores, local coralloid terminal hyphal cells, indeterminate thallus maturation, the presence of budding cells and local conversion to meristematic growth all indicate a relationship to the dothideaceae (dothideales, ascomycota). morphological properties were consistent with the genus hormonema lagerberg & melin, as defined by presence of percurrent conidi ...19979195004
abomasal nematodes in goats from the subtropical island of grand canary (spain).the prevalence of gastric nematodes in 151 goats on grand canary island is around 56%, with a mean burden of 691 worms/animal. no significant differences of prevalence and intensity were found between the four isoclimatic areas of the island [dry-desert (dd); dry-steppe (ds); temperate-mild (tm); temperate-cold (tc)]. five nematode species were identified, the most commonly-occurring being teladorsagia circumcincta (observed in 65.8% of parasitized animals) and trichostrongylus axei (51.9%). the ...19979208446
watching the canary: the prevention of suicide. 19979213144
molecular genetics of glucose-6-phosphate dehydrogenase deficiency in mexico.several studies carried out between 1965 and 1985 showed that g-6-pd deficiency in mexico is heterogeneous at the biochemical level and that the g-6-pd a- phenotype is relatively common. we have now investigated the molecular basis of g-6-pd deficiency in mexico. up-to-date 60 chromosomes with g6pd mutations have been studied, 16 in previous studies and 44 in the present work. molecular analysis of dna from g-6-pd deficient mexican mestizos and their relatives show that g-6-pd a- genotypes are r ...19979215753
a subtropical case of human babesiosis.this report constitutes the first well-documented case of symptomatic human babesiosis from a subtropical site, south of the 40th parallel. this paper describes the definitive identification of babesia divergens infection in a splenectomized patient from the canary islands.19979241387
re: phylogenetic relationships of the canary islands endemic lizard genus gallotia inferred from mitochondrial dna sequences: incorporation of a new subspecies. 19979242599
blood group polymorphisms in the canary islands.human samples from the seven canary islands were studied for the following polymorphic red cell blood group systems: abo, rh, mnss, fy and p. in contrast to the intra-insular homogeneity found, inter-insular heterogeneity was observed for abo, rh and fy. the observed blood group allelic systems were within the range of european populations, with some minor african contribution.19969263771
sensitization to blomia kulagini in a general population of a subtropical region of spain (canary islands).the blomia genus has been described as allergenic in man. the present study aimed to assess the prevalence of b. kulagini sensitization in a large population of allergic subjects without occupational exposure in a subtropical region (canary islands, spain). secondarily, a new standardized b. kulagini extract was evaluated. the study population comprised 207 patients. rast for b. kulagini was positive in 76.2% of patients, and 47 of them were selected for the biologic standardization. when the pr ...19979265987
selective expression of insulin-like growth factor ii in the songbird brain.neuronal replacement occurs in the forebrain of juvenile and adult songbirds. to address the molecular processes that govern this replacement, we cloned the zebra finch insulin-like growth factor ii (igf-ii) cdna, a factor known to regulate neuronal development and survival in other systems, and examined its expression pattern by in situ hybridization and immunocytochemistry in juvenile and adult songbird brains. the highest levels of igf-ii mrna expression occurred in three nuclei of the song s ...19979278533
identity and biodegradative abilities of yeasts isolated from plants growing in an arid climate.plants harvested in the canary islands lanzarote and fuerteventura were analyzed for the yeasts inhabiting their surface. half of the isolates (22 out of 44) were identified as debaryomyces hansenii. black ascomycetes, viz. hortaea werneckii and two hormonema species were represented by 7 strains. basidiomycetous yeasts, viz. cryptococcus sp. (8 strains), rhodotorula sp. (5 strains), cerinosterus cyanescens (1 strain) and pseudozyma sp. (1 strain) constituted a minority of 33%. thirty strains we ...19979298186
photoperiod regulation of neuron death in the adult canary.the avian brain undergoes naturally occurring cell death and neuronal replacement in adulthood. little is known about how neuron survival in adult birds is regulated. however, previous work suggests that this process is open to environmental control. we now report that a reduction in day length from spring-like to fall-like conditions can dramatically increase cell death in adult male canaries. many of the dying cells are projection neurons in the motor pathway controlling song learning and prod ...19979298761
expression of brain lipid binding protein in the brain of the adult canary and its implications for adult neurogenesis.brain lipid binding protein (blbp), a member of the fatty acid binding protein family, is expressed at high levels in the mammalian central nervous system during development, but not in adulthood. because the brain of adult birds continues to show significant levels of neurogenesis, we thought it likely that blbp expression would also be present. we used a polyclonal antibody against blbp to study the presence of this protein in the adult canary brain. this antibody stained 1) fibers and perikar ...19979300768
serum "b" esterases as a nondestructive biomarker for monitoring the exposure of reptiles to organophosphorus insecticides.a field study was conducted to validate serum b esterases as nondestructive biomarkers (ndbs) in lizards. serum butyrylcholinesterase (bche) and carboxylesterase (cbe) activities were measured in lizards and four species of birds collected in an area of 0.5 ha sprayed with 0.36 kg a.i./ha of folidol se5 (5% parathion). serum b esterase activities were determined in a total of 213 lizards (gallotia galloti) and 81 birds of four species (sylvia melanocephala, serinus canaria, parus caeruleus, and ...19979352213
intestinal cryptosporidiosis in pigeons (columba livia).an intestinal disease in pigeons (columba livia) from the canary islands characterized by diarrhea and body weight loss is described. intestinal cryptosporidiosis was identified in three young pigeons. cryptosporidia were associated with hyperplasia of the intestinal crypts and moderate inflammatory infiltration in lamina propria. this is the first report of cryptosporidiosis in pigeons.19979356726
distribution and dynamics in the expression of androgen and estrogen receptors in vocal control systems of songbirds.developmental and seasonal changes in the production of androgens and estrogens seem to control sex-specific differentiation and seasonal changes in sexual behaviors such as singing of songbirds. these steroids affect the brain by binding to intracellular located receptors. here we analyze whether the expression of androgen receptors (ar) and estrogen receptors (er) is a limiting factor for differentiation of the vocal pattern and the vocal control system of zebra finches and canaries. ar and er ...19979370218
oculopharyngeal muscular dystrophy in uruguay.within the last 30 years, sixty-five patients exhibiting the clinical symptoms of oculopharyngeal muscular dystrophy (opmd) were studied at the neuromuscular diseases unit of the neurological institute of montevideo. they are members of five unrelated families which came from the canary islands to uruguay between 1850 and 1900. in the three families examined, the typical inclusions characteristic of opmd were found in the nuclei of muscle fibers. treatment for ptosis and dysphagia was discussed. ...19979392016
epidemiological aspects of asthma in the canary islands. 19979416525
heeding warnings ... from the canary, the whale, and the jnuit, part two.there is a tension between traditional and modern definitions of reproductive risk and normalcy. these experts describe that tension as it plays out among the inuit of northern canada from the perspective of a community midwife who has worked with the inuit. she presents an analytical framework which classifies and illuminates the types of logic that compete in most birth settings around the world--a framework useful for showing how some types of logic can be supervalued while others, such as cu ...19979429437
primary neural precursors and intermitotic nuclear migration in the ventricular zone of adult canaries.new neurons continue to be born in the ventricular zone (vz) of the lateral ventricles in the brain of adult birds. on the basis of serial section reconstruction and electron microscopy, we determined that the vz of the adult canary brain is composed of three main cell types (a, b, and e). type a cells were never found in contact with the ventricle and had microtubule-rich processes typical of young migrating neurons. type b cells were organized as a pseudostratified epithelium, all contacted th ...19989437023
environmental toxicologic pathology--the canary went home. 19979437818
[q-fever imported into norway].q fever is an important zoonosis that occurs throughout the world. in contrast to most other european countries, there has been no evidence of endemic q fever in norway up to now. the disease is caused by coxiella burnetii, a rickettsia-like bacterium. humans are infected mainly by inhalation of contaminated aerosols from cattle, sheep and goats. clinical manifestations are protean, ranging from asymptomatic infection to life-threatening endocarditis. in this article we present the first four ca ...19979441420
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