Publications

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occurrence of sym-homospermidine in the japanese newt, cynops pyrrhogaster pyrrhogaster. 1979428556
studies on the formation and state of determination of the trunk organizer in the newt,cynops pyrrhogaster.the exact localization of the presumptive trunk organizer was determined by means of vital staining at the initiation of gastrulation (0 h embryo) and subsequently in 6, 9, 12 and 24 h embryos.the progressive changes in the self-differentiation and inductive capacity of the trunk organizer were studied in isolation cultures (sitting drop) and in sandwich cultures with competent gastrula ectoderm. in the 0 and 6 h embryo cultures the excised trunk organizer predominantly formed atypical ectoderm. ...197928304918
the effect of aging on the neural competence of the presumptive ectoderm and the effect of aged ectoderm on the differentiation of the trunk organizer incynops pyrrhogaster.the effect of aging on the neural competence of the presumptive ectoderm of the early gastrula, and the effect of aged ectoderm on the differentiation of the still uninvaginated dorsal blastoporal lip at the small yolk-plug stage - representing the trunk organizer - were examined by the sandwich method incynops pyrrhogaster.the presumptive ectoderm to be used as reaction system was taken from 0 to 36 h exogastrulae obtained by operation at the early gastrula stage and combined with trunk organiz ...197928304917
effect of starvation & hypophysectomy on amino acid levels of ovaries of triturus pyrrhogaster laur. 1978700806
on the vascular architecture of the carotid labyrinth in cynops pyrrhogaster and onychoactylus japonicus.the fine vascular arrangement of the carotid labyrinth in two species of urodeles, cynops pyrrhogaster and onychodactylus japonicus was investigated. india ink-gelatin injected preparations were examined under the dissecting microscope. resin casts of the blood vessels prepared using the method of murakami (1971) were observed in the scanning electron microscope. in both species, the common carotid artery terminated in the central chamber from which arose two retia mirabilia, i.e. an external ca ...1977597008
axon regeneration across the site of injury in the optic nerve of the newt triturus pyrrhogaster.the process by which axons regenerate following a freeze injury to the optic nerve of the newt was analyzed by light and electron microscopy. freezing destroys cellular constituents in a one millimeter segment of the nerve, leaving intact the basal lamina and the blood supply to the eye. no axons are seen at the site of injury one to seven days post lesion. this contrasts with the persistence of normal-appearing but severed unmyelinated axons within the cranial stump which thus give a false appe ...1977862013
electron microscopic stldy of the developing lateral-line organ in the embryo of the newt, triturus pyrrhogaster.the morphological sequence of events occurring in the development of the lateral-line organ was investigated in the embryo of the newt, triturus pyrrhogaster, by means of electron microscopy. six stages of development have been defined for convenience of description. the primordium of the lateral-line organ migrating from the pre- and post-auditory placodes is segmented into a small cell mass accompanied by a few schwann cells enveloping nerve fibers. in the clump of cells of the lateral-line pr ...19761008255
movement of the cell surface and change in surface area during cleavage in the newt's egg.in cleaving eggs of the newt, cynops pyrrhogaster, the behaviour of the egg surface was analysed by observing linear and area changes in a transplanted piece of cortex which differed in pigmentation from the background. results from the animal hemisphere showed that the surface in the cleavage plane shrank extensively in the region where the early cleavage furrow was being formed. on both sides of the cleavage plane, the surface began to shrink in a direction parallel to the cleavage furrow and ...1976987048
the relationship of a specific chromosomal region to the development of the acrosome.in early spermatids of urodeles the chromosome segments bearing constitutive heterochromatin are localized in one half of the round nucleus; this region becomes the basal part of the long nucleus of the spermatozoon. the euchromatic chromosome segments extend toward the anterior nuclear pole in a bouquet configuration (macgregor and walker, 1973). in the course of spermiohistogenesis, one of the heterochromatic regions (the acrosomal chromocenter) migrates from the basal part to the anterior hal ...1976949922
studies on the gastrulation of amphibian embryos: light and electron microscopic observation of a urodele cynops pyrrhogaster.the course of gastrulation in embryos of a urodele, cynops pyrrhogaster, was studied with 1 mum epon sections and transmission and scanning electron microscopy. during the initial period of gastrulation, the bottle cells and a groove are formed in the dorsal part. the outer ends of the bottle cells have many microvilli, an electron-dense layer and many small vesicles. microtubules are present parallel to the long axis of the bottle cells, and a yolk-platelet-free region is observed at the inner ...19751214113
[neural control of the terminal of the hindgut of triturus pyrrhogaster]. 19744477310
wave of stiffness propagating along the surface of the newt egg during cleavage.in the eggs of the newt, cynops (triturus) pyrrhogaster, change in stiffness of the cortex was measured in various regions at the time of the cleavage. measurements were performed by mitchison and swann's cell elastimeter method with a modification, in which two fine pipettes were attached to the surface of one egg at the same time, in order to compare the rigidity of two regions. the stiffness of the cortex changed very little before the start of the first cleavage. however, just before the app ...19744809240
self-differentiation of the heart rudiment of the newt (cynops pyrrhogaster). 19734797323
electron microscopic study on the parathyroid gland of the parathormone-injected newt, triturus pyrrhogaster (boie). 19734740812
spontaneous epidermal tumor in an adult newt, cynops pyrrhogaster. 19734689907
merits of the japanese newt, cynops pyrrhogaster pyrrhogaster, as an experimental animal, especially for the study of transplantation immunity. 19734609273
origin of histones, and relation between nuclear histones and cytoplasmic basic proteins in early development of japanese newt, triturus pyrrhogaster. 19725037000
ultrastructural change in the parathyroid gland of the phosphate treated newt, triturus pyrrhogaster (boié). 19715168592
[the electron microscopic study on the functional area of the digestive epithelial cell membrane in newt, triturus pyrrhogaster]. 19715290269
a nephroblastoma in a fire-bellied newt, cynops pyrrhogaster. 19704321086
regional histone changes in embryos of the newt, triturus pyrrhogaster, during early development. 19705459824
ultrasturcture of the parathyroid gland of the newt, triturus pyrrhogaster (boié) in the spring season. 19705468683
electron microscopic study on the parathyroid gland of the newt triturus pyrrhogaster (boié) in natural hibernation. 19705442608
alkaline phosphatase activity in the regenerating limb of the adult newt triturus pyrrhogaster. 19695384492
are the parathyroid glands necessary to the life of the newt, cynops pyrrhogaster? 19695395934
an electron microscopic study on the guanophore in the iris of the newt, triturus pyrrhogaster (boié). 19695395640
the mast cells in the brain of the triturus pyrrhogaster (boie). i. the histologic studies. 19684181658
mast cells and histamine in a newt, triturus pyrrhogaster boié. 19685711878
histochemical and electrophoretic studies of esterase in the regenerating forelimb of the adult newt, triturus pyrrhogaster. 19676075515
in vitro analyses of wolffian lens regeneration: differentiation of the regenerating lens rudiment of the newt, triturus pyrrhogaster. 19676075514
differentiation capacity of the marginal zone in the morula and blastula of triturus pyrrhogaster. 19676075513
electron microscopic examination of the sites of nuclear rna synthesis during amphibian embryogenesis.the site of h(3)-uridine incorporation and the fate of labeled rna during early embryo-genesis of the newt triturus pyrrhogaster were studied with electron microscopic autoradiography. isolated ectodermal and mesodermal tissues from the embryos were treated in h(3)-uridine for 3 hours and cultured in cold solution for various periods before fixation with oso(4) and embedding in epon. at the blastula stage, the only structural component of the nucleus seen in electron micrographs is a mass of chr ...196519866688
restitution of the eye from the iris after removal of the retina and lens together with the eye-coats in the newt, triturus pyrrhogaster. 196514346508
an observation of the mast cell, especially the dendrite-type tissue mast cell in triturus pyrrhogaster (boie). 196514289867
strontium and calcium reabsorption in renal tubules of the newt, triturus pyrrhogaster.reabsorption of calcium in the proximal tubule cannulated in vivo exceeded reabsorption of strontium. the diuretics diamox and salyrgan depressed the reabsorption of calcium but not strontium.196414107462
transmissibility and some histopathology of a spontaneously originated visceral tumor in the newt, triturus pyrrhogaster. 196314098909
the fine structure of tissue mast cells in triturus pyrrhogaster (boie). 196314046091
influence of ovarian extract upon the sex differentiation of terminal crest in the testis of triturus pyrrhogaster (boie). 196113904272
[membrane potential in kidney tubules of triturus pyrrhogaster]. 196114496151
on the commissura postoptica in the brain of a newt, triturus pyrrhogaster. 195513297330
cytological observations on the epithelium of the gallbladder in urodele amphibian triturus pyrrhogaster. 195414356692
ribonucleic acid and protein in the growing oocytes of triturus pyrrhogaster. 195313076194
the effect of x-rays on chromosomes and nucleoli in ovarian eggs of the salamander, triturus pyrrhogaster. 194720268160
genomic displacement and shift of the hybrid zone in the japanese fire-bellied newt.hybridizations on a secondary contact zone between 2 diverged lineages can have various evolutionary consequences, including the genetic replacement of one lineage by another. we detected such a case between 2 lineages (the central and western lineages) of the japanese fire-bellied newt, cynops pyrrhogaster in the chugoku district of western japan. we genotyped 269 individuals from 30 localities using the mitochondrial cytochrome b gene and 11 microsatellite loci. the mitochondrial dna (mtdna) a ...201829566204
comparative transcriptome reveal the potential adaptive evolutionary genes in andrias davidianus.to search the evidence of molecular evolution mechanism for aquatic and cave habitat in andrias davidianus, the evolution analysis was carried out among several species transcriptome data. the transcriptome data of notophthalmus viridescens, xenopus tropicalis, cynops pyrrhogaster, hynobius chinensis and a. davidianus were obtained from the genbank and reassembled except xenopus tropicalis. the blast search of transcriptome data obtained 1244 single-copy orthologous genes among five species. a p ...201829483849
species-specific and heterogeneous distribution of sialoglycoconjugates in the primary olfactory center of three species of asian salamanders (cynops).sialic acids (sia) are terminal components of glycoconjugates that are involved in molecular and cellular interactions in the olfactory system. diverse glycoconjugates are expressed in the salamander olfactory projection; however, their sialylation and the linkage of sia to underlying sugars remain largely unknown. the present study aimed to determine the expression of sia linked to galactose (gal)-n-acetylglucosamine and n-acetylgalactosamine (galnac) in the olfactory bulbs of three species of ...202032858481
embryonic epidermal lectins in three amphibian species, rana ornativentris, bufo japonicus formosus, and cynops pyrrhogaster.intelectins (itlns) are secretory lectins found in several chordate species that recognize carbohydrates on the bacterial cell surface depending on ca2+ . in newly hatched larvae of rana ornativentris (r. orn), bufo japonicus formosus (b. jpn), and cynops pyrrhogaster (c. pyr), an anti-itln monoclonal antibody (mab) labeled a subset of epidermal cells in whole-mount immunocytochemical assays. in western blot analyses, the mab identified protein bands at approximately 33-37 kda in the larval extr ...202032729712
localization of sperm intracellular ca2+ keeps fertilizability in the newt vas deferens.sperm intracellular ca2+ is crucial for the induction of sperm-egg interaction, but little is known about the significance of ca2+ maintenance prior to induction. in sperm of the newt cynops pyrrhogaster, intracellular ca2+ is localized to the midpiece during storage in the vas deferens, while extracellular ca2+ is influxed in modified steinberg's salt solution to promote a spontaneous acrosome reaction related to the decline of sperm quality. in the present study, sperm from the vas deferens we ...202031940274
identification and characterization of pou class v family genes in japanese red bellied newt, cynops pyrrhogaster.mammalian pou5f1 encodes the pou family class v (pou-v) transcription factor which is essential for the pluripotency of embryonic cells and germ cells. in vertebrates, various pou-v family genes have been identified and classified into the pou5f1 family or its paralogous pou5f3 family. in this study, we cloned two cdnas named cppou5f1 and cppou5f3, which encode pou-v family proteins of the japanese red bellied newt cynops pyrrhogaster. in the predicted amino acid sequence encoded by cppou5f1, th ...201931412969
enhancement of the olfactory response by lipocalin cp-lip1 in newt olfactory receptor cells: an electrophysiological study.previously, we have detected the expression of 2 lipocalin genes (lp1 and lp2) in the olfactory epithelium of the japanese newt cynops pyrrhogaster. recombinant proteins of these genes (cp-lip1 and cp-lip2, respectively) exhibited high affinities to various odorants, suggesting that they work like the odorant-binding proteins (obps). however, the physiological functions of obp generally remain inconclusive. here, we examined the effect of cp-lip1 on the electrophysiological responses of newt olf ...201931346612
some aspects of the hypothalamic and pituitary development, metamorphosis, and reproductive behavior as studied in amphibians.in this review article, information about the development of the hypothalamo-hypophyseal axis, endocrine control of metamorphosis, and hormonal and pheromonal involvements in reproductive behavior in some amphibian species is assembled from the works conducted mainly by our research group. the hypothalamic and pituitary development was studied using bufo embryos and larvae. the primordium of the epithelial hypophysis originates at the anterior neural ridge and migrates underneath the brain to fo ...201931238076
nmda-type glutamate receptors mediate the acrosome reaction and motility initiation in newt sperm.the n-methyl d-aspartate type glutamate receptor (nmdar) is a ligand-gated cation channel that causes ca2+ influx in nerve cells. an nmdar agonist is effective to the sperm motility in fowls, although the actual role of nmdar in sperm function is unknown. in the present study, rna-seq of the spermatogenic testes suggested the presence of nmdar in the sperm of the newt cynops pyrrhogaster. glutamate of at least 0.7 ±â€‰0.5 mm was detected in the egg-jelly substances along with acrosome reaction-ind ...201931215127
acrosome reaction-inducing substance triggers two different pathways of sperm intracellular signaling in newt fertilization.the acrosome reaction is induced in the sperm of cynops pyrrhogaster immediately in response to a ligand protein called acrosome reaction-inducing substance (aris) in the egg jelly at fertilization, whereas a spontaneous acrosome reaction occurs time-dependently in correlation with the decline of sperm quality for fertilization. the aris-induced acrosome reaction was recently found to be mediated by trpv4 in association with the nmda type glutamate receptor, although the intracellular mediators ...201932149368
skin mucosome activity as an indicator of batrachochytrium salamandrivorans susceptibility in salamanders.recently emerged fungal diseases, batrachochytrium dendrobatidis (bd) and batrachochytrium salamandrivorans (bsal) are an increasing threat to amphibians worldwide. in europe, the threat of bsal to salamander populations is demonstrated by the rapid decline of fire salamander populations in germany, the netherlands and belgium. although most european urodelans are susceptible to infection in infection trials, recent evidence suggests marked interspecific differences in the course of infection, w ...201830020936
effects of growth hormone and nutrient on limb regeneration in hypophysectomized adult newts.the effect of hypophysectomy, growth hormone (gh) and an amino acid-glucose mixture on the regenerative ability of the hypophysectomized triturus pyrrhogaster yielded the following results: 1 the survival time of hypophysectomized newts can be prolonged substantially by the sulfamide application. 2 although the limb regeneration in the hypophysectomized newt is retarded as compared with that of the pituitary intact control, it finally completes morphogenetic process under such conditions of prol ...197330332863
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