| [specificity of carp antihuman myeloma protein sera depending on the holding temperature]. | while in carps kept in warm water (28 degrees c) and immunized with a human myeloma protein a challenge leads to an increase, the antibody titres in carps kept in cold water (14 degrees c) are changed only unsignificantly. antibody titres of carps immunized once respectively three times and kept in cold water are not markedly different. in testing the specificity of the antisera taken from each of the three groups no differences could be recognized. with carp anti-iggzei sera using the passive h ... | 1985 | 2936221 |
| biology and histopathology of thelohanellus hovorkai achmerov, 1960 (myxosporea, myxozoa), a protozoan parasite of the common carp (cyprinus carpio). | | 1986 | 2953194 |
| applicability of coagglutination test for detecting aeromonas salmonicida antigens from extracts of bacterial cultures and experimentally infected tissues of the common carp (cyprinus carpio l.). | | 1986 | 2953200 |
| transferability of immune plasma and pronephric cells in isogeneic, allogeneic and xenogeneic transfer systems in crucian carp. | transfer of immunity to horse erythrocytes (hrbc) by intravascular injection of immune plasma and pronephric cells was performed using histocompatible and histoincompatible crucian carp to examine "mhc" regulation in adoptive immunity. clonal ginbuna crucian carp, carassius gibelio langsdorfii and outbred kinbuna crucian carp, c. carassius buergeri immunized with hrbc were used as donors of plasma and cells for transfer to unimmunized members. recipients were then evaluated by measuring the anti ... | 1987 | 2960574 |
| reexamination of the evidence for paramagnetism in oxy- and carbonmonoxyhemoglobins. | experiments have been carried out in an attempt to confirm previous reports of paramagnetism in the oxy- and carbonmonoxy derivatives of human and carp hemoglobin. when care is taken to ensure complete saturation of the hemoglobins with ligand and the diamagnetic contributions of all of the buffers are carefully evaluated, these hemoglobin derivatives are found to have the same gram susceptibilities as exhibited by a set of metal free proteins, suggesting that they are fully diamagnetic. | 1985 | 2982138 |
| human chorionic gonadotrophin and immature fish ovary: characterization and mechanism of the in vitro stimulation of cyclic adenosine monophosphate accumulation. | human chorionic gonadotrophin (hcg) increased cyclic adenosine monophosphate (camp) in pieces of eel ovary in vitro. although the ed50 (about 0.1 micrograms/ml) was the same as for carp gonadotrophin (cgth), the maximal stimulation (close to 3 times basal level) was about 10 times lower than that for the fish hormone. the mechanism of this atypical action of hcg was studied using different approaches. human cg did not inhibit the action of cgth. desensitization experiments (cgth or hcg being inj ... | 1985 | 2985465 |
| purification and properties of carp (cyprinus carpio) muscle calpain ii (high-ca2+-requiring form of calpain). | calpain (ca2+-dependent cysteine proteinase) was purified to apparent homogeneity from carp muscle by the method of deae-cellulose, hydroxylapatite and ultrogel aca 34 column chromatographies. the purified enzyme is classified as calpain ii (high-ca2+-requiring form of calpain) from the effects of ca2+ concentration, ph and the antibiotics on the activity. carp muscle calpain ii was inhibited by rat liver calpastatin, the specific inhibitor for calpain. it is probable that the calpain-calpastati ... | 1985 | 2992871 |
| mutual inhibitory effect of calpastatins on calpains from carp muscle, carp erythrocytes and rat liver. | the inhibitory effect of calpastatin (specific inhibitor for calpain) on calpain (ca2+-dependent cysteine proteinase, ec 3.4.22.17) was examined using carp muscle, carp erythrocytes and rat liver preparations. a mutual inhibitory effect between calpains and calpastatins from different tissues and species was observed. the conservation of the inhibitory effect of calpastatin on calpain among vertebrates suggests that the calpain-calpastatin system may play a biologically fundamental and common ro ... | 1985 | 2992872 |
| viral diseases of fish: first report of carp pox in golden ide (leuciscus idus) in north america. | carp pox, a putative viral disease exotic to north america, occurred in golden ide 1 yr after the fish were imported into the united states from the federal republic of germany. the raised, white, plaque-like lesions, which occurred on about 5% of the fish, healed spontaneously and caused no mortality. electron micrographs showed herpesvirus-like particles associated with lesion specimens; however, no infectious viruses were detected in tests with seven warmwater fish cell lines. | 1985 | 2993681 |
| internally transposed signal sequence of carp preproinsulin retains its functions with the signal recognition particle. | it is shown that the signal sequence of carp preproinsulin is functional with the dog pancreatic signal recognition particle (srp) both when present at its normal location at the amino-terminus of the protein or when engineered to an internal location. inhibition of translation by srp in the absence of microsomal membranes, reconstitution by srp of the translocation competence of high-salt inactivated microsomes and signal peptide cleavage all occur with the signal sequence being preceded by a h ... | 1986 | 3000822 |
| a signal sequence is required for the functions of the signal recognition particle. | it is shown that removal of the signal sequence of carp preproinsulin by gene technology abolishes the interaction between the polypeptide chain and the signal recognition particle with respect to both its functions: the inhibition of translation by the signal recognition particle in the absence of microsomal membranes, and the reconstitution of the protein translocation competence of high-salt inactivated microsomes. it is concluded that a signal sequence is absolutely required for the function ... | 1986 | 3004463 |
| complete nucleotide sequence of the matrix protein mrna of vesicular stomatitis virus (new jersey serotype). | the complete nucleotide sequence of the mrna of the matrix (m) protein of vesicular stomatitis virus [new jersey serotype, vsv(nj)] was derived from a cdna clone and mrna. the mrna is 758 nucleotides long (excluding polyadenylic acid) and encodes a protein of 229 amino acids. the predicted amino acid sequence was compared with that of the corresponding protein of indiana serotype [vsv(ind)] and a fish rhabdovirus, spring viremia of carp virus (svcv). an amino acid identity of 62% was found betwe ... | 1986 | 3006343 |
| [accumulation of cyclic adenosine monophosphate in the ovary of the eel (anguilla anguilla l.) in vitro under the effect of carp gonadotropin or ovine lutropin: kinetics and thermodependence]. | cyclic amp (camp) in pieces of eel ovary was greatly increased in vitro by the gonadotropin (cgth) of carp, another teleost fish; after one hour at 20 degrees c, maximal stimulation = 31.7 and e.d. 50 = 0.08 micrograms/ml. ovine lutropin (olh) had less effect (maximal stimulation: 2.35; e.d. 50: 1.42 micrograms/ml); its action suggested that it involved a subfraction (olh/cgth rac) of the receptor-adenylate cyclase (rac) systems which mediate the action of cgth. another difference was the percen ... | 1986 | 3012723 |
| carp, a novel gene regulating the transcription of the carbamoylphosphate synthetase operon of escherichia coli. | the carab operon, encoding carbamoylphosphate synthetase (cpsase; ec 6.3.5.5) is transcribed from two tandem promoters. the upstream promoter (p1) is controlled by pyrimidines and the downstream promoter (p2) is controlled by arginine. we have isolated a new type of constitutive mutation (carp) that specifically affects the control of the pyrimidine-sensitive promoter but does not appear to influence other genes of the pyrimidine pathway. the carp mutation acts in trans and is dominant, which su ... | 1988 | 3065518 |
| [immunoenzyme (elisa) diagnosis of spring viremia in carp]. | the enzymoimmunologic (elisa) method was used to diagnose spring viremia in carp. the specific antibodies to rhabdovirus carpio in the fish sera were detected by this method. reaction specificity was tested by the use of viral and control antigens, by examination of positive and negative sera and by serum-free control incubation. applying the elisa method, the antiviral antibodies can be detected after infection or vaccination: this method is therefore suitable for thorough studies and when choo ... | 1986 | 3094221 |
| immunological relationship among glycogen-protein complexes of different species. | glycogen-protein complexes from the muscles of celerio euphorbiae and horse as well as from the livers of frog, carp and man were isolated and used as antigens. after immunization of rabbits antisera were obtained from which fractions of immunoglobulins were isolated. antibodies were used in agar immunodiffusion tests and in quantitative reaction of immunoprecipitation with glycogens. the immunoprecipitation test proved the occurrence of quantitative differences in cross reaction of complexes wi ... | 1986 | 3109350 |
| ultrastructural characterization of leucocytes in the pronephros of carp (cyprinus carpio, l.). | in the pronephros of carp (cyprinus carpio, l.) the following cells were found and ultrastructurally characterized: erythrocytes; lymphocytes and plasma cells; thrombocytes; neutrophilic, eosinophilic and basophilic granulocytes; phagocytic reticular cells and monocytes and non-phagocytic reticular cells. the cells could be separated on a percoll continuous density gradient and relatively pure fractions could be obtained. in vitro phagocytosis of bacteria (bacillus megaterium) was found in monoc ... | 1987 | 3109968 |
| increased gonadotropin levels in goldfish do not result in alterations in circulating thyroid hormone levels. | to determine whether increases in gonadotropin levels are capable of altering thyroid function in goldfish, plasma thyroid hormone levels were measured following induced changes in endogenous gonadotropin secretion and injection of carp gonadotropin. radio-frequency lesions placed in the nucleus preopticus periventricularis or monosodium-l-glutamate-induced lesions of the posterior nucleus lateralis tuberis (nlt) of the hypothalamus were capable of stimulating significant increases in plasma gon ... | 1987 | 3114043 |
| identification and immunocytochemical localization of two different carbonic anhydrase isoenzymes in teleostean fish erythrocytes and gill epithelia. | carbonic anhydrase was purified from the gills (cab) of the rainbow trout salmo gairdneri and from erythrocytes (cae) of the fresh water carp cyprinus carpio. the purification of the isozymes was confirmed by sds acrylamide gel electrophoresis. antibodies against the purified cab and cae were then raised in rabbits. specificity was verified by immunoblotting. no cross-reaction was found between them, using the immunodot technique. cab antiserum was used to specifically localize gill ca in the tr ... | 1988 | 3139588 |
| nucleotide sequence of chicken myb proto-oncogene promoter region: detection of an evolutionarily conserved element. | the nucleotide sequence of the chicken myb proto-oncogene putative promoter region was determined and compared with the corresponding sequence of the mouse c-myb gene (1). 118 bp upstream from the initiation codon suggested by gerondakis and bishop (2) for the chicken c-myb protein, a 124-bp-long conserved element was found (92% identity in chicken and mouse sequences). sequences homologous to this element were detected on southern blots of restricted genomic dnas from mouse, man, lizard, frog, ... | 1988 | 3145493 |
| localization of immunoreactive synthetic atrial natriuretic factor (anf) in the heart of various animal species. | the localization of two synthetic fragments of the c-terminal portion of atrial natriuretic factor: arg 101-tyr 126 which displays full biological activity and leu 94-arg 109 which is completely devoid of biological activity, has been investigated by immunohisto- and immunocytochemical methods in the heart of mammals (rat, mouse, guinea pig, hamster, rabbit, cat, dog, man) and nonmammalian vertebrates toad (bufo marinus), frog (rana catesbeiana), fish (cyprinus carpio, puntius schwanenfeldi, cic ... | 1985 | 3158698 |
| effect of manganese ions on the immune response of carp (cyprinus carpio) against yersinia ruckeri. | the effect of manganese ions mn2+ (mn cl2) on hematocrit and the antibody response of carp (cyprinus carpio) was investigated after exposure of fish to contaminated water for 2.5 months. contamination of water with 50 mg/l mn2+ did not decreased antibody production against yersinia ruckeri (measured by agglutination) but significantly decreased hematocrit. the levels of manganese in lymphoid organs (spleen and kidney) increased progressively during the experiment. the effect of manganese in vitr ... | 1988 | 3169354 |
| dna:dna reassociation analysis of aeromonas salmonicida. | dna from 26 aeromonas salmonicida strains, namely 11 'typical' and 15 so-called 'atypical' strains, was used to assess the taxonomic relatedness within the species. the genomes were characterized by determination of dna base composition, dna:dna reassociation, calculation of sequence divergence following reassociation, and by genome size estimations. by comparison with dna obtained from controls and the aeromonas hydrophila group, a. salmonicida strains were determined to be correctly placed wit ... | 1988 | 3171541 |
| comparative biotransformation of molinate (ordram) in the white sturgeon (acipenser transmontanus) and common carp (cyprinus carpio). | 1. juvenile white sturgeon (acipenser transmontanus) and common carp (cyprinus carpio) were exposed to 100 micrograms/l [ring-14c]molinate in a flow-through metabolism system. exposure was in three phases: acclimation (2 h), uptake (24 h), and depuration (24 h). 2. excreted metabolites were collected on a macroreticular resin, and retained metabolites were extracted from homogenized fish tissue. identification and quantification was by h.p.l.c. cochromatography (gradient conditions) and determin ... | 1988 | 3176521 |
| [the seasonal dynamics of sphaerospora renicola (myxosporidia) in carp (osteichthyes)]. | we observed 2 different types of the course of incidence of sporogonic phase and blood phase. type i shows in both phases a maximum in the first summer, a minimum in winter or autumn and a second maximum between late autumn and second summer. in this case the sporulation was especially distinct with the first maximum. type ii starts in the first summer with the blood phase without occurrence of the sporogonic phase (and the swim-bladder phase), which appears only from autumn until the second sum ... | 1988 | 3177937 |
| carp gamma-crystallins with high methionine content: cloning and sequencing of the complementary dna. | the nucleotide sequences of gamma-crystallin cdnas cloned from the common carp (cyprinus carpio) have been determined. the amino-acid sequences derived consist of two polypeptides with 177 and 172 amino-acid residues for gamma-m1 and gamma-m2, respectively. they exhibit unusually high methionine contents: 12.4% for gamma-m1 and 14% for gamma-m2. comparison of both fish gamma-crystallins with bovine gamma-ii crystallin reveals that they are similar in structure. the striking features of both fish ... | 1988 | 3191133 |
| the complete amino acid sequences of beta-subunits of two distinct chum salmon gths. | the complete amino acid sequences of beta-subunits of chum salmon (oncorhynchus keta) gonadotropin (gth) i and gth ii were determined. both gth i beta and gth ii beta were treated with neuraminidase, and then reduced and carboxymethylated. both of the modified subunits were digested separately with several endopeptidases. the resulting fragments were purified by reversed-phase high-performance liquid chromatography and subjected to sequence analysis by manual edman degradation. gth i beta was a ... | 1988 | 3192067 |
| interactions between human and carp (cyprimus carpio) low density lipoproteins (ldl) and ldl receptors. | 1. we have compared the concentration and chemical composition of carp and human plasma lipoproteins and studied their interaction with human fibroblast ldl receptors. 2. the main lipoproteins in carp are of high density (hdl) in contrast to low density lipoproteins (ldl) in human. 3. carp lipoproteins are devoid of apolipoprotein (apo) e, a major ligand for interaction with ldl receptors in mammals. 4. carp very low density lipoproteins (vldl) and ldl but not hdl nor apoa-i cross react with hum ... | 1988 | 3197401 |
| daily cycles in plasma melatonin levels under long or short photoperiod in the common carp, cyprinus carpio. | a radioimmunoassay (ria) for plasma melatonin (mlt) was simplified for use with the common carp (cyprinus carpio). plasma was partially purified with sep-pak c18 cartridge before ria. the inhibition curves for the sep-pak c18 fraction from the plasma of carp, goldfish (carassius auratus), yellow tail (seriola quinqueradiata), japanese eel (anguilla japonica), bora (mugil cephalus cephalus), kisu (sillago japonica), ishigarei (kareius bicoloratus), and shimaisaki (rhyncopelates oxyrhynchus) were ... | 1988 | 3197949 |
| dopamine cells and rod bipolar cells contain protein kinase c-like immunoreactivity in some vertebrate retinas. | the localization of cells immunoreactive to a monoclonal antibody against protein kinase c (pkc) and to polyclonal antibodies against tyrosine hydroxylase (th) was investigated in the retina of fish (carp, goldfish, dace and catfish), frog, turtle, chick and some mammalians (guinea pig, rat, cat and rabbit) by means of fluorescence microscopy. pkc-like immunoreactivity was found in dopamine (da) or th-like immunoreactive (ir) cells in all the species examined and also in rod bipolar cells in the ... | 1988 | 3205402 |
| seasonal variations of glucose and triiodothyronine concentrations in serum of carp (cyprinus carpio l). | thyroid hormone and glucose serum concentrations (sc) of carps (cyprinus carpio l) have been monitored at weekly intervals throughout the year. t4 and rt3 concentrations were always below the limit of detection of the assays applied. t3 and glucose mean sc showed seasonal variations. highest t3 sc were reached in summer with a peak value in august and lowest values in winter with a maximum in december. glucose sc have been found to be highest in winter and lowest in summer. statistical analysis ... | 1988 | 3220447 |
| a comparative study of aldehyde dehydrogenase and alcohol dehydrogenase activities in crucian carp and three other vertebrates: apparent adaptations to ethanol production. | in the final step of the pathway producing ethanol in anoxic crucian carp (carassius carassius l.), acetaldehyde is reduced to ethanol by alcohol dehydrogenase. the presence of aldehyde dehydrogenase in the tissues responsible for ethanol production could cause an undesired oxidation of acetaldehyde to acetate coupled with a reduction of nad+ to nadh. moreover, acetaldehyde could competitively inhibit the oxidation of reactive biogenic aldehydes. in the present study, the distribution of aldehyd ... | 1988 | 3220990 |
| trace element residues in bluegills and common carp from the lower san joaquin river, california, and its tributaries. | whole-body samples of bluegills (lepomis macrochirus) and common carp (cyprinus carpio) from the san joaquin river and two tributaries (merced river and salt slough) were analyzed to determine if the concentrations of any of nine elements were elevated as a result of exposure of the fish to agricultural subsurface (tile) drainage water. highest concentrations (micrograms/g dry weight) detected were as follows (first number in each pair applies to bluegills and the second to carp): arsenic (as), ... | 1988 | 3222692 |
| [residues of active substances after consumption of contaminated food: status report on the evaluation of residues with examples of two drugs]. | pharmacokinetic models are presented for the computation of time courses of blood levels of drugs in man following the consumption of contaminated food. mathematically, two linear systems of differential equations are set up for the donor organism (e.g., trout) and for the recipient, (e.g., man), where the first system generates the initial conditions for the second. models of this kind are applied to the transfer of chloramphenicol to man via carp and trout (which had previously been administer ... | 1988 | 3223091 |
| purification and some properties of glyceraldehyde-3-phosphate dehydrogenase from carp muscle. | glyceraldehyde-3-phosphate dehydrogenase was purified from carp white muscle. on cm-sephadex chromatography two well separated active peaks were obtained. both of them show a single protein band on gel electrophoresis and have the same molecular and kinetic properties; they differ only by the amount of bound nad, the enzyme in the second peak being coenzyme-free. significant differences were observed between the properties of carp and pig muscle enzymes. glyceraldehyde-3-phosphate dehydrogenase ... | 1988 | 3232465 |
| xenobiotic metabolizing enzyme systems in test fish. iii. comparative studies of liver cytosolic glutathione s-transferases. | the reduced glutathione (gsh)-conjugating capacities of the livers of trout, carp, zebra fish, guppy, and bluegill have been evaluated with several substrates preferentially metabolized by the major gsh-transferase isozymes. carp and zebra fish lack gsh-transferase activity with 1,2-epoxy-3-(p-nitrophenoxy)propane. guppy is characterized by the highest activities with 1-chloro-2,4-dinitrobenzene and the absence of activity toward trans-4-phenyl-3-buten-2-one. enzyme activities with the other sub ... | 1988 | 3234293 |
| a comparative trial of garlic, its extract and ammonium-potassium tartrate as anthelmintics in carp. | while fish chemotherapy is not a common practice in méxico, folk medicine is usually well accepted by fish farmers. empirical knowledge suggested that garlic should have anthelmintic properties. various trials were carried out in cyprinus carpio infested with capillaria sp., using minced garlic and its extracts. an empirical concentration of 200 mg/l of minced garlic showed the greatest activity against the above mentioned worms (100%). the hexane extract in equivalent amounts showed a 75% effec ... | 1988 | 3253490 |
| the protein sequence homology of gamma-crystallins among major vertebrate classes and their dna sequence homology to heat-shock protein genes. | a systematic characterization of lens crystallins from five major classes of vertebrates was carried out by exclusion gel filtration, cation-exchange chromatography and n-terminal sequence determination. all crystallin fractions except that of gamma-crystallin were found to be n-terminally blocked. gamma-crystallin is present in major classes of vertebrates except the bird, showing none, or decreased amounts, of this protein in chicken and duck lenses, respectively. n-terminal sequence analysis ... | 1988 | 3255376 |
| comparative studies on the structure of biliary immunoglobulins of some avian species. ii. antigenic properties of the biliary immunoglobulins of chicken, turkey, duck and goose. | studies on the immunological cross-reactivity of the igs+ from the bile of chicken and turkey (order galliformes) as well as duck and goose (order anseriformes) to igs with antigenic known properties carried out by a series of ria. various class-specific anti-ig sera produced in rabbits, guinea-pigs and mice were used. the following results were obtained: (1) a high extent of antigenic relationship between the chicken and turkey biliary igs was demonstrated by a radiobinding system consisting of ... | 1988 | 3260198 |
| adaptational change in cone-mediated electroretinogram in human and carp. | | 1988 | 3265993 |
| the fetal phenotype of the 18p-syndrome. report of a male fetus at twenty-one weeks. | morphological and cytogenetic findings in a male fetus at 21 weeks gestation after prenatally detected monosomy 18p are reported. the fetus displayed dysmorphic features resembling the 18p-syndrome, such as decreased head circumference, slightly receding forehead, hypertelorism, epicanthus, horizontal palpebral fissures, depressed nasal bridge, long philtrum, carp mouth, irregular crenated maxillar alveolar ridge, retrognathia, lowset dysplastic ears with posterior rotation, edema of neck, hands ... | 1988 | 3281573 |
| cloning and sequencing of a carp beta s-crystallin cdna. | the mrnas were extracted from common carp (cyprinus carpio) lenses, purified, reverse transcribed, dc tailed and cloned into escherichia coli with pbr322 as vector. the cloning efficiency was around 1 x 10(7) colonies per micrograms of mrna. a clone (pc20) was found by hybrid-arrested to contain the cdna related to carp crystallins. however, comparison of the derived amino-acid sequence with bovine gamma-ii and beta s-crystallins indicates that this carp crystallin sequence resembles closely the ... | 1987 | 3307924 |
| primate specific sialoglycoprotein of sperm head plasma membrane defined by an anti-carbohydrate monoclonal antibody. | a sialoglycoprotein, an integral component of the head plasma membrane of human spermatozoa, is recognized by the a-hs 1a.1 monoclonal antibody. the antigenicity is associated with the sugar moiety since: a) trypsin digestion did not affect the antigenic determinant; b) pretreatment of the cells with beta-glucosidase, alpha-mannosidase and neuraminidase completely abolished antibody binding. endoglycosidase d and glycopeptidase f were inactive. the a-hs 1a.1 did not recognize a variety of blood- ... | 1987 | 3317219 |
| [sphaerospora renicola (myxosporidia), the agent of protozoal swim bladder inflammation in young carp (renicola sphaerosporosis)]. | | 1987 | 3328529 |
| properties of odour-binding glycoproteins from rat olfactory epithelium. | the specific membrane glycoproteins with high affinity for camphor and decanal were isolated from rat olfactory epithelium. antibodies to these glycoproteins inhibited both the electroolfactogram and the binding of odorants. the enzyme immunoassay has shown these glycoproteins to be present in the olfactory epithelium of rat, mouse, guinea-pig and hamster but not in that of frog and carp. the molecular mass of the odour-binding glycoproteins from rat olfactory epithelium solubilized by triton x- ... | 1988 | 3337807 |
| cutaneous tumor of northern pike, esox lucius l. ii. expression of surface and cytoplasmic immunoglobulins and alpha-naphthyl acetate esterase by mononuclear cells from lymphoid tissues and tumor. | rabbit anti-pike igm antibodies showed that up to 95% of mononuclear (mn) cells isolated on ficoll-isopaque gradients from peripheral blood, spleen, and head kidney of northern pikes carrying cutaneous tumors were surface (sig) and cytoplasmic (cig) immunoglobulin positive by indirect immunofluorescence. on the contrary, rabbit anti-winter flounder igm antibodies gave totally negative results. monoclonal anti-carp igm antibodies stained approximately 40-56% of peripheral blood, splenic, and head ... | 1988 | 3350193 |
| species differences in 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity and biotransformation in fish. | rainbow trout, yellow perch, carp, bluegill, largemouth bass, and bullhead were treated with graded doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd; 1, 5, 25, or 125 micrograms/kg) or vehicle, ip. the lethal potency of tcdd tended to be greater in yellow perch, carp, and bullhead than in the other three species (ld50 80 days post-treatment, 3-5 versus 10-16 micrograms/kg, respectively). all species treated with the highest dose of tcdd (125 micrograms/kg) displayed a latency period of 1-4 wee ... | 1988 | 3356307 |
| gonadotropin content of the pituitary gland of gonadal tumor-bearing common carp x goldfish hybrids from the great lakes, as assessed by bioassay and radioimmunoassay. | the hybrids of carp (cyprinus carpio) and goldfish (carassius auratus) collected from the lower great lakes between 1978 and 1981 exhibited epizootics of gonadal neoplasm which were rare in the parental species. the pituitary glands of hybrids were, on average, 2.5 times larger than that of carp of similar body size. histologically, the hypertrophy was due to hyperplasia of proximal pars distalis basophils (the presumptive gonadotropes). using a carp gonadotropin (gth) radioimmunoassay it was fo ... | 1988 | 3366360 |
| isolation and properties of the pituitary gonadotropin from silver carp (hypophthalmichthys molitrix). | the gonadotropin of silver carp (scgth) was purified from the pituitary glands in a yield of 8.4 mg/g through extraction with 6% ammonium acetate (ph 5.1)-40% ethanol followed by 80% ethanol precipitation, deae-cellulose chromatography and gel filtration. the molecular weight of scgth is about 32,000 as estimated by gel filtration. leucine and serine were the two n-termini of scgth. the amino acid composition of scgth is rich in aspartic acid/asparagine, glutamic acid/glutamine, valine, proline ... | 1988 | 3366548 |
| monoclonal antibodies directed against the calcium binding protein parvalbumin. | we have produced twelve monoclonal antibodies (mcab) against carp-ii parvalbumin. three of them, designated 235, 239, 267 recognize determinants conserved in fish, chicken, mouse, rat, monkey and human parvalbumin. we show their use in the qualitative detection of parvalbumin (pv) by immunohistochemistry, in the quantitation of parvalbumin by radioimmunoassay and in the detection of parvalbumin on immunoblots. | 1988 | 3383226 |
| experimental verification of the effect of the form (scaly or scaleless) of carp host (c. carpio l.) on the growth rate of micropopulations of gyrodactylus katharineri malmberg, 1964 (monogenea). | it was demonstrated that the growth rate of gyrodactylus katharineri micropopulations parasitizing the fry of cyprinus carpio l. was effected by water temperature and by the form of the fish host (scaly or scaleless). at lower water temperature (12 degrees c) the number of parasites increased in both host forms approximately at the same intensity. in warmer water (18 degrees c), the parasites reproduced intensively only on the scaly form of fish, whereas no parasites were found on the scaleless ... | 1987 | 3428761 |
| interaction of mycoplasma mobile 163k with erythrocytes. | mycoplasma (m.) mobile 163k isolated from fish was investigated for its hemadsorbing, hemagglutinating, and hemolysing capacities and for its ability to adhere to erythrocytes. hemadsorption to the colonies of m. mobile occurred with ovine, bovine, equine, trout, and carp erythrocytes and was inhibited by treatment of the mycoplasmas with substances acting on proteins (pronase, trypsin, glutaraldehyde), heat, uv-irradiation and homologous antiserum. hemadsorption could be prevented also by treat ... | 1987 | 3439386 |
| environmental changes induced by growth-associated triggering factors in injured optic nerve of adult rabbit. | central nervous system (cns) neurons of mammals regenerate poorly after axonal injury. however, if an injured cns neuron (rabbit optic nerve) is supplied with appropriate soluble substances ("growth-associated triggering factors") derived from medium conditioned by regenerating fish optic nerve or newborn rabbit optic nerve, it can express regeneration-associated characteristics. such characteristics include a general increase in protein synthesis, changes in synthesis of specific polypeptides, ... | 1987 | 3470808 |
| distribution of pancreatic elastase and metalloproteinase in vertebrates. | elastase-like enzymes were detected as zymogens in all of the pancreatic extracts from the gummy shark, bullhead shark, angel shark, smooth hammerhead, bestel, rainbow trout, carp, eel, japanese mackerel, yellowtail, sea bass, parrotfish, bullfrog, chicken, bluewhite dolphin, hog, rat, cat, and dog. the distribution of pancreatic elastase and metalloproteinase was examined on the basis of the effect of specific inhibitors on elastase like-activity in each extract. the results indicate that pancr ... | 1986 | 3484693 |
| phylogenetic studies on lymphokines. fish lymphocytes respond to human il-1 and epithelial cells produce an il-1 like factor. | the results obtained indicate, that catfish peripheral blood lymphocytes recognize and respond to human il-1. the second part of this report is dealing with a substance produced by carp epidermal cells with functional similarities to mammalian il-1. | 1986 | 3490047 |
| cadmium-binding protein (metallothionein) in carp. | when carp (cyprinus carpio) were exposed to 5 and 30 ppm cd in the water, the contents of cd-binding protein, which has low molecular weight, increased in the hepatopancreas, kidney, gills and gastrointestinal tract with the duration of exposure. this cd-binding protein was purified from hepatopancreas, kidney, gills, and spleen of carp administered 2 mg/kg cd (as cdcl2), intraperitoneally for 6 days. two cd-binding proteins were separated by deae-sephadex a-25 column chromatography. these prote ... | 1986 | 3519201 |
| immunohistochemical localization of urotensins i and ii in the caudal neurosecretory neurons of the carp cyprinus carpio and the sharks heterodontus japonicus and cephaloscyllium umbratile. | using antisera to urotensins i and ii (ui and uii), in the carp, cyprinus carpio, three types of caudal neurosecretory neurons were identified: those with both ui- and uii-immunoreactivities, those with only ui-immunoreactivity and those with only uii-immunoreactivity. the last type of neurons exceeded the other types in number, while neurons immunoreactive with both ui and uii antisera were relatively few. the axons of neurons of these three types terminated around the capillaries in the urophy ... | 1986 | 3524503 |
| neuropeptide y (npy) immunoreactive neurons in the retina of different species. | neurons displaying neuropeptide y (npy) immunoreactivity were found among amacrine cells in the retina of baboon, pig, cat, pigeon, chicken, frog, trout, carp and goldfish. the immunoreactive cell bodies were located in the middle and the innermost cell rows of the inner nuclear layer with processes forming one, two or three more or less well-defined sublayers in the inner plexiform layer. the location and the density of the sublayers varied with the species investigated. in the frog retina, bip ... | 1986 | 3546220 |
| the influence of environmental temperature on the lymphocyte populations in carp (cyprinus carpio l.). | the influence of environmental temperature upon the lymphocyte populations in carp (cyprinus carpio l.) has been studied by immunoperoxidase labeling using different antisera. in carps kept in a warm and a cool environment, respectively, rabbit anti-carp igm as well as rabbit anti-carp fc antiserum showed different percentages of ig-positive lymphocytes, whereas the percentage remained unaffected by temperature after the use of rabbit anti-carp fab antiserum with a specificity against l-chains. ... | 1987 | 3555478 |
| the thyroxine-stimulating activity is only present in the glycoprotein and not in the protein fraction of a carp hypophyseal homogenate. | the in vivo release of thyroxine (t4) in the carp (cyprinus carpio) and the in vitro release of t4 in the frog (rana ridibunda) from isolated thyroids were used as a bioassay to study the thyrotropic activity of carp hypophyses. the addition of up to 1 hypophyseal equivalent to the medium did not increase the in vitro release of t4 from isolated frog thyroids in november whereas 3 mu of bovine thyrotropin (b-tsh) was effective. however, when this experiment was repeated at the end of february, t ... | 1986 | 3557057 |
| yersinia enterocolitica, yersinia frederiksenii and yersinia intermedia in cyprinus carpio (linneo 1758). | | 1987 | 3591113 |
| homeothermic fish and hemoglobin: primary structure of the hemoglobin from bluefin tuna (thunnus thynnus, scromboidei). | some fish are warm-bodied, e.g. the bluefin tuna (thunnus thynnus), which has a muscle temperature 12-17 degrees c higher than its environment. this endothermy is achieved by aerobic metabolism and conserved by means of a heat-exchanger system. the hemoglobins of bluefin tuna are adapted to these conditions by their endothermic oxygenation, thus contributing to the preservation of the body energy. this is a new and so far unique property of tuna hemoglobin. the primary structure of the alpha and ... | 1987 | 3620111 |
| intrinsic fluorescence of carp hemoglobin: a study of the r----t transition. | the intrinsic fluorescence of hemoglobins is known to respond to ligand-induced changes in the quaternary structure of the protein. carp hemoglobin is an interesting model to study the quaternary transition since its r----t equilibrium is ph-dependent and at low ph, in the presence of organic phosphate, it remains in the t or 'deoxy' quaternary structure, even when saturated with ligand. in this study, using front-face fluorometry, we show that the intrinsic fluorescence intensity exhibited by c ... | 1987 | 3620472 |
| inhibitory effect of carp muscle trypsin inhibitor on mammalian pancreatic proteinases. | carp muscle trypsin inhibitor showed an inhibitory effect on bovine trypsin, bovine alpha-chymotrypsin and porcine elastase in a non-competitive, competitive and competitive manner, respectively. the inhibitor formed a stable complex with the above proteinases which was not dissociated in the presence of 2-mercaptoethanol and sds. the true target proteinase for carp muscle trypsin inhibitor, as yet unknown, seems to be an alpha-chymotrypsin- or elastase-like enzyme rather than trypsin, judging f ... | 1986 | 3633776 |
| in-vitro binding of gonadotrophin to fish ovary. | binding of piscine and mammalian gonadotrophin to plasma membranes from the ovaries of a fish, the murrel (channa punctatus), clearly suggests that the fish ovary possesses distinct and specific binding sites for both piscine and mammalian gonadotrophins. maximum specific binding of 125i-labelled human chorionic gonadotrophin (125i-hcg) and 125i-labelled silver carp gonadotrophin (125i-scg) was obtained at 30 degrees c and ph 7.5 during 2 h of incubation. in competitive binding studies, binding ... | 1986 | 3643250 |
| contribution to the knowledge of gyrodactylus gurleyi price, 1937 (monogenea: gyrodactylidae). | gyrodactylus longoacuminatus zitnan, 1964, forma minor sensu ergens (1985) was identified as g. gurleyi price, 1937. morphological and metrical evaluations of the hard parts of opisthaptor of more than 200 specimens provided data on the variability of this parasite; cyprinus carpio haematopterus represents its new host. since the shape of the hard parts of opisthaptor of g. gurleyi is very similar to that of g. longoacuminatus, further detailed studies of the two species will be necessary, parti ... | 1987 | 3666611 |
| first record of sphaerospora renicola dyková et lom, 1982 and myxobolus encephalicus mulsow, 1911, pathogenic protozoans of carp from the u.s.s.r. | | 1987 | 3666614 |
| 1h nmr spectroscopic studies of calcium-binding proteins. 2. histidine microenvironments in alpha- and beta-parvalbumins as determined by protonation and laser photochemically induced dynamic nuclear polarization effects. | the microenvironments of the histidines in three isoforms of ca(ii)-bound parvalbumin (carp, pi = 4.25; pike, pi = 5.00; rat, pi = 5.50) have been examined with 1h nmr techniques to probe their protonation characteristics and photochemically induced dynamic nuclear polarizability (photo-cidnp). the histidine at position 26 (or 25), present in all three of these proteins, shows absolutely no photo-cidnp enhancement of its c2h or c5h resonances. nor does this nonpolarizable histidine possess a nor ... | 1986 | 3707913 |
| resonance raman spectroscopic evidence that carp deoxyhemoglobin remains in a t-like quaternary structure at high ph: implications for cooperativity. | resonance raman spectroscopy shows the fe-proximal imidazole stretching band to shift from 215 to 219 cm-1 between human deoxyhemoglobin (deoxy-hb) and a hb sample which is 75% oxygenated, demonstrating that the t-r quaternary structure switch can be monitored by resonance raman spectroscopy in native hb at equilibrium. for deoxy-hb from carp, the band is at 215 cm-1 at ph 9 as well as ph 6, contrary to previous reports of an elevated frequency at high ph. the invariance of this frequency over a ... | 1986 | 3707921 |
| characterization of lens crystallins and their mrna from the carp lenses. | crystallins from carp eye lenses have been isolated and characterized by gel permeation chromatography, sds-gel electrophoresis, immunodiffusion and amino acid analysis. gamma-crystallin is the most abundant class of crystallins and constitutes over 55% of the total lens cytoplasmic proteins. it is immunologically distinct from the alpha- and beta-crystallins isolated from the same lens and its antiserum shows a very weak cross-reaction to total pig lens antigens. comparison of the amino acid co ... | 1986 | 3707973 |
| annual cyclical changes in the testicular activity of an indian freshwater major carp, labeo rohita (hamilton). | different male germ cells identified on the basis of histological and cytological characteristics in the testicular lobules of labeo rohita have been grouped into spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa. the seasonal changes of the testis in l. rohita have been described according to its morphological peculiarities as well as to its variations in gonadal volumes, gsi values and frequency percentages of the different male germ cells occurring in t ... | 1986 | 3710120 |
| galactosyl-binding lectins from the tunicate didemnum candidum. purification and physicochemical characterization. | the plasma of the ascidian didemnum candidum possesses lectin activity directed toward galactosyl moieties. we report the purification by affinity chromatography, the physicochemical properties, amino acid composition, and partial n-terminal amino acid sequence of two galactosyl-binding lectins d. candidum lectins i and ii (dcl-i and dcl-ii) from the plasma of this protochordate species. both lectins were purified by affinity chromatography (on acid-treated sepharose 4b and asialofetuin conjugat ... | 1986 | 3722194 |
| radioimmunoassays for fish tail neuropeptides: i. development of assay and measurement of immunoreactive urotensin i in catostomus commersoni brain, pituitary, and plasma. | antisera were raised in rabbits using highly purified urotensin i (ui) or ui (4-41) conjugated with high-molecular-weight proteins. antiserum 2u4, at a final dilution of 1:300,000, gave 50% binding to iodinated ui (specific activity 40-120 microci/micrograms). carp (cyprinus carpio) and sucker (catostomus commersoni) ui peptides cross-reacted completely with the antiserum. results from cross-reactivity tests using various tryptic and staphylococcus aureus protease fragments of ui indicated that ... | 1986 | 3724203 |
| protein digestion and amino acid absorption along the intestine of the common carp (cyprinus carpio l.), a stomachless fish: an in vivo study. | protein hydrolysis to peptides and free amino acids and the apparent absorption of amino acids (aaaa) were evaluated in different segments of carp intestine. the aaaa analysed using cr2o3 as a marker indicated that 73.2% of the amino acids were absorbed in the first 20% of the intestinal tract and 5.3 and 21.5, respectively, in the following segments (20% of gut length). except for methionine and histidine, essential free amino acid concentration decreased significantly along the intestine. of t ... | 1986 | 3749594 |
| pronephric leucocytes of cyprinus carpio: isolation, separation and characterization. | since the teleost pronephros is an important source of diverse immunocytes, suspensions of pronephric cells from young adult carp have been characterized. in freshly prepared suspensions, adherent, spreading cells (macrophages?) constituted less than 3% of the total population. granulocytes and lymphocytes were co-dominant (less than 80%) leucocyte types. continuous percoll density gradient centrifugation yielded discrete subpopulations with these rho values and cytological characteristics: frac ... | 1986 | 3765337 |
| blood changes in carp (cyprinus carpio) induced by ulcerative aeromonas salmonicida infections. | only recently aeromonas salmonicida has been recognized as a significant bacterial pathogen in ulcerative disease of cyprinid fish. our attempts to formulate a vaccine based on bacterial surface antigens were unsuccessful in conferring reliable protection against lethal challenge. this lead us to study pathological changes in the humoral defense system during ulcerative a. salmonicida infection in carp. high numbers of opportunist pathogens such as a. hydrophila and pseudomonas sp. were frequent ... | 1986 | 3765352 |
| does aeromonas salmonicida affect the immune system of carp, cyprinus carpio l.? | aeromonas salmonicida is a significant bacterial pathogen of cyprinid and salmonid fishes causing the systemic disease furunculosis. several observations led us to believe that a. salmonicida was able to evade or suppress the immune system of the fish: injection of whole bacteria or surface antigens was unsuccessful at protecting fish against lethal challenges; memory did not develop in survivors of sublethal infections; diseased fish often carried other opportunistic bacterial pathogens in addi ... | 1986 | 3765353 |
| the influence of environmental temperature and infection route on the immune response of carp (cyprinus carpio) to spring viremia of carp virus (svcv). | carp infected by water route and by injection of svcv developed neutralizing antibodies within 2-8 weeks at water temperatures between 10-20 degrees c. at 10 degrees c svcv persisted 12 weeks in the blood of carp although antibodies were present. immunized carp showed a solid protective immunity to svcv. | 1986 | 3765360 |
| analysis of zeros of binding polynomials for tetrameric hemoglobins. | a quantitative measure of the validity of the mwc description of cooperative binding equilibria has been obtained which uses only the adair constants. this is accomplished through simple relationships using the zeros of the adair binding polynomial and unique properties of the zeros of mwc polynomials as described in the accompanying paper (w.e. briggs, biophys. chem. 24 (1986) 311). the method is applied to oxygen binding to a large number of hemoglobins under a wide variety of conditions. in m ... | 1986 | 3768473 |
| immunization of carp (cyprinus carpio) with a vibrio anguillarum bacterin: indications for a common mucosal immune system. | uptake and transport of formalin-killed vibrio anguillarum bacteria were studied in the second gut segment of carp and the resulting reaction of the immune system was investigated. within a few hours after anal administration antigenic determinants of bacteria were present in intraepithelial macrophages of the second gut segment. in gut and skin mucus and bile, immunoglobulins (ig's) were detected, but the amount was much lower than found in serum. in mucus of the second gut segment, 4 times mor ... | 1986 | 3770268 |
| species variation of gonadotropic activity in an in vitro assay measuring androgen formation by carp (cyprinus carpio) testis with special reference to bioassay of piscine gonadotropins. | the objectives of this study were to establish a suitable and validated in vitro bioassay of piscine gonadotropins (gths) by using a carp testis androgen production system and to compare the androgenic responses in such an assay to gonadotropins from various vertebrate species. the testes from mature carp with gonadosomatic indices of 8-30% were used. androgen production was first compared with respect to methods for preparation of the carp testis (sliced, minced, homogenized, and collagenase-di ... | 1986 | 3770434 |
| [interaction of histone h1 molecules in a solution]. | molecules of histones h1 isolated from the calf thymus, carp testicles and spermatozoa as well as trypsin-stable fragments of these proteins have been studied from the standpoint of their structure and interaction using methods of differential spectrophotometry, gel filtration and turbidimetry. the globular structure of histone h1 of the calf thymus is formed with an increase in the ionic strength of the medium and it is eluted as dimer with gel chromatography. with a considerable local increase ... | 1986 | 3775878 |
| development and validation of a salmon growth hormone radioimmunoassay. | a highly specific and sensitive radioimmunoassay (ria) for the measurement of plasma and pituitary growth hormone (gh) levels in salmonid fishes was developed using an anti-serum raised in rabbit against chum salmon (oncorhynchus keta) gh (sgh). pituitary extracts and plasma from chum, coho, masu, and amago salmon, and from rainbow trout and japanese charr, all exhibited displacement curves parallel to the sgh standard. samples from the eel, carp, goldfish, and tilapia, as well as plasma from hy ... | 1986 | 3781223 |
| morphology of physiologically identified bipolar cells in the retina of the tiger salamander, ambystoma tigrinum. | intracellular recordings of the light responses of bipolar cells were made in the isolated perfused retina of the larval tiger salamander by microelectrodes filled with a 1% solution of the enzyme horseradish peroxidase (hrp). two classes of bipolar cell were identified in terms of their responses to luminous spots and annuli centered upon their receptive fields: on-center cells, which depolarized in response to a centered spot and hyperpolarized in response to a concentric annulus, and off-cent ... | 1986 | 3793974 |
| the amino-terminal sequences of four major carp gamma-crystallin polypeptides and their homology with frog and calf gamma-crystallins. | four major gamma-crystallin subfractions have been isolated from the carp (cyprinus carpio) and their n-terminal sequences determined by edman protein sequencing. extensive homologies indicative of close relatedness in their primary structure were found in these four gamma-crystallin polypeptides. comparison of the carp n-terminal sequences with those of mammalian and amphibian gamma-crystallins also showed a high degree of homology present in their n-terminal segments despite the dissimilarity ... | 1986 | 3803569 |
| [clinical picture of partial monosomy of chromosome 11 q]. | a ten months old male infant was found to have a de-novo-deletion of the long arm of chromosome 11 (q23----qter). with regard to previous reports the combination of the following signs seems to be pathognomonic for partial monosomy of the long arm of chromosome 11: trigonocephaly, abnormal palpebral fissure, epicanthus, broad and depressed nasal bridge, carp shaped mouth, low-set malformed ears and statomotoric retardation. cerebral atrophy, bns-seizures, hypsarrhythmia and persistent leukocytop ... | 1986 | 3807920 |
| mechanism of synthesis and localization of mitochondrial and cytosolic fumarases in rat liver. | fumarases in the mitochondrial and cytosolic fractions of rat liver were separately purified and crystallized. these two fumarases were not distinguishable in physicochemical, catalytic, or immunochemical properties. the sequences of seven amino acids in the c-terminal portions of the two fumarases were shown using carboxypeptidase p to be identical, i.e.-val-asp-glu-thr-ala-leu-lys-. the amino acid sequence of the n-terminal portion of the mitochondrial fumarase was determined by the edman meth ... | 1986 | 3812085 |
| cation binding to parvalbumin studied by 113cd and 23na nmr. peak assignment of rabbit (pi 5.5) parvalbumin. | cation binding to three apoparvalbumins was studied by means of 113cd nmr. the 3 parvalbumins that were investigated were carp pi 4.25, rabbit pi 5.5 and pike pi 5.0. the results showed that cd2+ ions bind to the ef and cd sites of carp apoparvalbumin pi 4.25 with about the same affinity. for rabbit (pi 5.5) apoparvalbumin, cd2+ binds preferentially to the ef site, while for pike (pi 5.0) apoparvalbumin, it was the cd site that exhibited somewhat higher affinity for cd2+. the effect of mn2+ on t ... | 1986 | 3825402 |
| xenobiotic-metabolizing enzyme systems in test fish. i. comparative studies of liver microsomal monooxygenases. | all monooxygenase activities assayed in guppy (poecilia reticulata) and red killifish (oryzias latipes) were 7-10 times higher than those measured in trout (salmo gairdneri), carp (cyprinus carpio), and golden orfe (leuciscus idus). cytochrome p-450 was 3 times higher in the former fish than in all the other species. zebra fish (brachydanio rerio) 7-ethoxycoumarin o-deethylase and bluegill (lepomis macrochirus) ethylmorphine n-demethylase (emnd) were not greatly different from those of the forme ... | 1987 | 3830019 |
| quantitation and immunohistochemistry of catecholamines in the posterior segment of the eye. | dopamine, noradrenaline, and adrenaline were assayed with hplc in the light adapted retinae of carp, frog, chicken, pigeon, rat, guinea-pig, rabbit, cat, pig and cow. dopamine varied from 0.6 to 2.6 nmol/g wet weight and was not influenced by sympathectomy. the dopamine figures agree with previously published results. noradrenaline concentrations varied from not detectable to 0.06 nmol/g wet weight in different species. homolateral sympathectomy significantly decreased the noradrenaline figure i ... | 1985 | 3875596 |
| urotensin ii-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish. | antiserum generated against synthetic urotensin ii of the goby, gillichthys mirabilis, was used to localize urotensin ii in the caudal neurosecretory system in six species of freshwater teleosts: cyprinus carpio, carassius auratus, oreochromis mossambicus, oreochromis niloticus, salmo gairdneri and plecoglossus altivelis, and six species of seawater teleosts: acanthogobius flavimanus, pagrus major, parapristipoma trilineatum, trachurus japonicus, seriola dumerili and seriola quinqueradiata. in t ... | 1985 | 3884162 |
| immunohistochemical localization of chick retinal 24 kdalton protein (visinin) in various vertebrate retinae. | antiserum against a protein (24,000 daltons, visinin) of chick retina has been provided for immunohistochemical study on the localization of visinin in chick retinae during development, as well as in various vertebrate retinae. the photoreceptor cells were stained with anti-visinin serum from 7th day embryonic retinae and its intensity was gradually increased with embryonic age. in addition, visinin-like immunoreactivity was found in some kinds of amacrine and displaced amacrine cells from 11th- ... | 1985 | 3886079 |
| skeletal muscle actin as tumor marker in the diagnosis of rhabdomyosarcoma in childhood. | immunoaffinity-purified and tissue-specific antibodies against carp skeletal muscle actin have been assessed for their usefulness in diagnosing rhabdomyosarcoma. routinely processed formaldehyde-fixed tissue and the avidin--biotinyl--peroxidase complex technique were used. thirty-six tumors of patients varying in age from less than 1 year to 17 years were diagnosed as rhabdomyosarcoma on the basis of routine histological stains or electron microscopy, and on clinical grounds. among them were 20 ... | 1985 | 3911779 |
| microspectrophotometric and scanning microphotometric studies of carp (cyprinus carpio l.) erythrocytes. | carp (cyprinus carpio) hemoglobin readily autoxidizes in blood smears. quantification of soret-band absorbance in individual erythrocytes by means of scanning cytophotometry therefore requires more elaborate methods of preparation of blood samples. of the fixatives that have been tested, suspension of whole blood in isotonic salt solutions containing glutaraldehyde was most suitable. glutaraldehyde-fixed red blood cells are totally resistant to hemolysis. in the course of fixation, hemoglobin is ... | 1985 | 3928536 |
| opposite effects of ammonia and carbon dioxide on dye coupling between horizontal cells in the carp retina. | effects of ammonia (nh3) and carbon dioxide (co2) on the membrane potential of horizontal cells and on dye coupling between the cells in isolated retinas of the carp (cyprinus carpio) were investigated. ammonia (less than 300 ppm nh3 in air) initially depolarized and subsequently hyperpolarized, while co2 (10% in air) hyperpolarized the membrane potential of horizontal cells, accompanied by a diminution of both center and surround responses to spot and annular light stimuli. during the course of ... | 1985 | 3929998 |
| [reproduction of ichthyophthirius multifiliis (ciliata, ophryoglenidae)]. | a case of reproduction of ichthyophthirius multifiliis in the superficial tissues of larvae and fry of amur wild carp is described. at a temperature of water from 28 to 29 degrees trophonts of ichthyophthirius encysted on fishes. inside cysts repeated cell division occurred but this process did not result in swarm spores formation. later on with the increase of temperature to 29.5--31.5 degrees cysts degenerated. | 1985 | 3937118 |
| thermodynamics of carp metmyoglobin unfolding. | the conformational free energy of carp lateral muscle metmyoglobin at 25 degrees c ph 8 is 9.0 +/- 0.5 kcal/mol as estimated from isothermal unfolding by both urea and guanidinium chloride. a novel procedure for the simultaneous analysis of acid and guanidinium chloride unfolding data is described. acid denaturation data suggest that binding of five protons to histidyl residues occurs on unfolding. correlation of sequences and conformational stabilities of several myoglobins according to the ter ... | 1986 | 3956157 |
| geminate recombination of co in rabbit, opossum, and adult hemoglobins. | the geminate recombination of co with hb following dissociation by a 10-ns laser pulse has been studied as a function of ph (9.2 and 7.0 without inositol hexaphosphate and 6.0 with inositol hexaphosphate) and temperature (5-35 degrees c). the hemoglobins studied included adult, rothschild, rabbit, opossum, and carp. despite significant differences in their structural and functional properties, the first four of these hemoglobins show similar trends in the yields, rates, and activation energies o ... | 1985 | 3972802 |
| [adaptibility of diplostomum cercariae in carp and the effect of a prior infestation]. | experiments on the infection of carp fry with cercariae of diplostomum have shown that cercariae of diplostomum spathaceum can penetrate crystalline lens of larvae of these fishes in three days after their hatching and that the adaptability of cercariae of d. paracaudum to carp is much higher than that of cercariae of d. spathaceum. after the first infection with cercariae a relative postinfectious immunity against infection with cercariae of this or close species of this genus arises in carp fr ... | 1985 | 3975067 |
| non-specific cytotoxic activity of teleost leukocytes. | the existence of lymphoid cells with "natural" killer activity in mammals and birds has been known for some time. several previous reports have demonstrated similar activity in carp and catfish kidney leukocytes. however, the activity previously reported was directed towards established mammalian cell lines. in this report we confirm the existence of spontaneous killer activity in other species of teleosts, including salmonids. this spontaneous cytotoxic activity is directed towards several esta ... | 1985 | 3996708 |