| alteration of the sialylation pattern and memory deficits by injection of aß(25?35) into the hippocampus of rats. | sialic acid in glycoconjugates participates in important cellular functions associated with normal development, growth, and communication. therefore we evaluated the sialylation pattern and memory deficits caused by the injection of aß((25-35)) into the hippocampus (hp) of rats. the eight-arm maze spatial-learning and memory test indicated that the injection of aß((25-35)) into subfield ca1 of the hp impaired both learning and memory. the sialylation pattern was examined using sialic acid-specif ... | 2011 | 21419829 |
| effect of nutrition and enteromyxum leei infection on gilthead sea bream sparus aurata intestinal carbohydrate distribution. | the effect of a practical plant protein-based diet containing vegetable oils (vo) as the major lipid source on the mucosal carbohydrate pattern of the intestine was studied in gilthead sea bream sparus aurata challenged with the myxosporean parasite enteromyxum leei. fish fed for 9 mo either a fish oil (fo) diet or a blend of vo at 66% of replacement (66vo diet) were exposed to parasite-contaminated water effluent. samples of the anterior, middle and posterior intestine (ai, mi and pi, respectiv ... | 2012 | 22885511 |
| human red blood cell aging: correlative changes in surface charge and cell properties. | red blood cells (rbcs) during microcirculation, aging and storage, lose n-acetylneuraminic acid(nana) and other biomaterials thereby altering cell structures, some properties and functions. such cell damage very likely underlies the serious adverse effects of blood transfusion. however, a controversy has remained since 1961-1977 as to whether with aging, the rbcs, suffering loss of nana, do have a decreased charge density. any correlation between the changes in the cell properties with cell agin ... | 2011 | 21435169 |
| entomotoxic action of sambucus nigra agglutinin i in acyrthosiphon pisum aphids and spodoptera exigua caterpillars through caspase-3-like-dependent apoptosis. | in this project, the toxicity and mechanism of action of the ricin-b-related lectin sna-i from elderberry (sambucus nigra) in the pea aphid (acyrthosiphon pisum) and the beet armyworm (spodoptera exigua), two important pest insects in agriculture, were studied. sna-i is a chimeric lectin belonging to the class of ribosome-inactivating proteins and consists of an a-chain with n-glycosidase activity and a carbohydrate-binding b-chain. incorporation of 2 mg/ml of sna-i in the diet of neonates and a ... | 2010 | 20853435 |
| early murine t-lymphocyte activation is accompanied by a switch from n-glycolyl- to n-acetyl-neuraminic acid and generation of ligands for siglec-e. | it is well established that murine t-lymphocyte activation is accompanied by major changes in cell-surface sialylation, potentially influencing interactions with sialic acid-binding immunoglobulin-like lectins (siglecs). in the present study, we analysed early activation of murine cd4+ and cd8+ t-lymphocytes at 24 h. we observed a striking and selective up-regulation in the binding of a recombinant soluble form of siglec e, an inhibitory siglec which is expressed on several myeloid cell types in ... | 2011 | 21835922 |
| medicinal plants used for dermatological affections in navarra and their pharmacological validation. | this paper provides significant ethnopharmacological information on plant used in dermatological affections in navarra. | 2013 | 23892205 |
| enrichment of sialylated igg by lectin fractionation does not enhance the efficacy of immunoglobulin g in a murine model of immune thrombocytopenia. | intravenous immunoglobulin g (ivig) is widely used against a range of clinical symptoms. for its use in immune modulating therapies such as treatment of immune thrombocytopenic purpura high doses of ivig are required. it has been suggested that only a fraction of ivig causes this anti immune modulating effect. recent studies indicated that this fraction is the fc-sialylated igg fraction. the aim of our study was to determine the efficacy of ivig enriched for sialylated igg (ivig-saôü¦) in a muri ... | 2011 | 21731683 |
| medicinal plants used for ophthalmological problems in navarra (spain). | several plants have been found to have effective against number of ophthalmological problems in navarra. | 2016 | 27269391 |
| biochemical, molecular and structural analysis of multiple thaumatin-like proteins from the elderberry tree (sambucus nigra l.). | thaumatin-like proteins (tlps) were isolated and characterized from fruits and leaves of elderberry (sambucus nigra) and their corresponding genes cloned. in addition, the developmental regulation and induction of the different tlps was followed in some detail. ripening berries accumulated a fruit-specific tlp during the final stages of maturation. this fruit-specific tlp had no antifungal activity and was devoid of beta-glucanase activity. leaves constitutively expressed a tlp that closely rese ... | 2002 | 11941461 |
| fucose and sialic acid expressions in human seminal fibronectin and α{1} -acid glycoprotein associated with leukocytospermia of infertile men. | the aim of this study was to compare fucose and sialic acid residue expression on fibronectin and α{1}-acid glycoprotein in the seminal plasma of men suspected of infertility and suffering from leukocytospermia. | 2011 | 22048274 |
| anthocyanin-rich black elderberry extract improves markers of hdl function and reduces aortic cholesterol in hyperlipidemic mice. | serum high-density lipoprotein-cholesterol (hdl-c) is a risk factor considered to be protective of atherosclerosis. however, atherosclerosis is an inflammatory disease and contributes to impairment in high-density lipoprotein (hdl) function, including reductions in hdl-c, hdl antioxidant and anti-inflammatory activities. anthocyanins are polyphenols that have demonstrated antioxidant and anti-inflammatory properties. the objective of this study was to determine whether an anthocyanin-rich black ... | 2015 | 25758596 |
| differential expression of o-glycans in cd4(+) t lymphocytes from patients with systemic lupus erythematosus. | t cells from patients with systemic lupus erythematosus (sle) show a decreased activation threshold and increased apoptosis. these processes seem to be regulated by glycosylated molecules on the t cell surface. here, we determined through flow cytometry the expression of mucin-type o-glycans on t helper cells in peripheral blood mononuclear cells (pbmc) from 23 sle patients and its relation with disease activity. we used lectins specific for the disaccharide gal-galnac, such as amaranthus leucoc ... | 2016 | 27600584 |
| terminal glycotope expression on milk fibronectin differs from plasma fibronectin and changes over lactation. | fibronectin (fn) is a multifunctional glycoprotein appearing in various glycovariants with potential biological activities. using lectins we analyzed the expression of terminal glycotopes on human milk fibronectin over lactation and compared it with that of the mother's plasma. | 2015 | 25464017 |
| neu1 sialidase regulates the sialylation state of cd31 and disrupts cd31-driven capillary-like tube formation in human lung microvascular endothelia. | the highly sialylated vascular endothelial surface undergoes changes in sialylation upon adopting the migratory/angiogenic phenotype. we recently established endothelial cell (ec) expression of neu1 sialidase (cross, a. s., hyun, s. w., miranda-ribera, a., feng, c., liu, a., nguyen, c., zhang, l., luzina, i. g., atamas, s. p., twaddell, w. s., guang, w., lillehoj, e. p., puché, a. c., huang, w., wang, l. x., passaniti, a., and goldblum, s. e. (2012) neu1 and neu3 sialidase activity expressed in ... | 2014 | 24550400 |
| complex thiolated mannose/quinone film modified on eqcm/au electrode for recognizing specific carbohydrate-proteins. | a complex thiolated mannose (tm)/quinone functionalised polythiophene (qfpt) thin film was modified on eqcm/au electrode for recognition of specific carbohydrate-proteins. different lectins such as those from sambucus nigra (elder berry), arachis hypogaea (peanut), ulex europaeus (gorse, furze), triticum vulgaris and concanavalin a (cona) was used for probes to evaluate bio-sensing performance of the tm/qfpt film. a specific response was observed for cona from lectins when using the tm/qfpt film ... | 2014 | 24373955 |
| sialylated and o-glycosidically linked glycans in prion protein deposits in a case of gerstmann-sträussler-scheinker disease. | prion diseases are caused by an abnormal form of the prion protein (prp(sc)). we identified, with lectins, post-translational modifications of brain proteins due to glycosylation in a gerstmann-sträussler-scheinker (gss) patient. the lectin amaranthus leucocarpus (all), specific for mucin type o-glycosylated structures (galß1,3 galnacα1,0 ser/thr or galnacα1,0 ser/thr), and sambucus nigra agglutinin (sna), specific for neu5acα2,6 gal/galnac, showed positive labeling in all the prion deposits and ... | 2011 | 20667006 |
| ruptured achilles tendons show increased lectin stainability. | to ascertain whether lectins could be a useful tool for investigation of the extracellular matrix of degenerated and normal tendons. | 2002 | 12131241 |
| hypersialylated macromolecular serum immunoglobulin a1 in type 2 diabetes mellitus. | the origin of the elevation of serum immunoglobulin a1 (iga1) in type 2 diabetes mellitus (dm) is unsettled. the aim of this study was to address the carbohydrate changes of serum iga1 from patients with type 2 diabetes mellitus, as a possible cause of the elevation. | 2001 | 11239513 |
| grading dysplasia in colorectal adenomas by means of the quantitative binding pattern determination of arachis hypogaea, dolichos biflorus, amaranthus caudatus, maackia amurensis, and sambucus nigra agglutinins. | the current study deals with the setting up of a new tool that enables the benign versus the malignant nature of colorectal adenomas to be determined accurately. the 2 objectives are to determine (1) whether adenomas should, or should not, be included in a 2- or a 3-tier grading system, and (2) whether severe dysplasias and carcinomas in situ share common or different biological characteristics. the levels of expression of different types of glycoconjugates were characterized in a series of 166 ... | 1999 | 10534165 |
| alterations in the o-linked glycosylation of iga1 in children with henoch-schönlein purpura. | to examine o-linked glycosylation of serum iga1 in children with acute henoch-schönlein purpura (hsp). | 1997 | 9375892 |
| changes of glycoprotein patterns in sera of humans under stress. | stress exhibits adverse effects on many vital processes in which glycoproteins play a significant role(e.g. cell-cell/matrix interactions, immune response, neoplastic growth, implantation, prenatal development), yet only scarce attention has been directed towards studying stress induced changes in glycoprotein patterns. using sds-electrophoresis, blotting and digoxigenin-labelled lectins (sambucus nigra agglutinin, galanthus nivalis agglutinin, datura stramonium agglutinin, maackia amurensis agg ... | 1996 | 8833640 |
| the use of streptavidin-biotinylglycans as a tool for characterization of oligosaccharide-binding specificity of lectin. | a new rapid and sensitive method for characterizing lectin specificity using streptavidin-biotinylglycans as a tool is presented. this assay is analogous to enzyme immunoassay and takes advantage of the strong, irreversible adsorption of streptavidin to the wells of the chambers of titer plates. a series of streptavidin-biotinylglycans was first coated on a microtiter plate, and then one of six lectins, concanavalin a, wheat germ agglutinin, phaseolus vulgaris (red kidney bean) erythro-agglutini ... | 1992 | 1443561 |
| identification of a monoclonal antibody to abscission tissue that recognises xylose/fucose-containing n-linked oligosaccharides from higher plants. | monoclonal antibodies raised against extracts of the rachis abscission zone of sambucus nigra l. were selected for high reactivity towards abscission-zone proteins. one antibody (yz1/2.23) has been shown to cross-react, by both indirect and competition enzyme-linked immunosorbent assay and by western blotting, with a number of plant enzymes including horseradish peroxidase, rice α-glucosidase, almond β-glucosidase and the lectins from phaseolus vulgaris and erythrina cristagalli.the major n-link ... | 1988 | 24221933 |
| the interaction of selective plant lectins with neuraminidase-treated and untreated iga1 from the sera of iga nephropathy patients. | a study on the binding interaction of lectins from artocarpus heterophyllus (jacalin), glycine max and sambucus nigra with standardised quantity of iga from the iga nephropathy patients and normal controls was performed. the glycine max lectin demonstrated higher affinity towards the serum iga of igan patients as compared to normal controls. however, the affinity binding was lower in cases ofjacalin and the sambucus nigra lectin. when serum samples were treated with neuraminidase, the differenti ... | 2001 | 11419909 |
| the quality of selected south african and international homoeopathic mother tinctures. | the high potential variability of chemical composition of the plant material involved in the manufacture of homoeopathic mother tinctures (a common source of homoeopathic medicines), renders both their quality control and assurance a significant challenge (pande and pathak, 2006). the absence of significant regulations regarding the quality of complementary and alternative medicines (cam) in south africa contributes to this challenge (gqaleni et al, 2007). in order to assess any quality differen ... | 2011 | 22754057 |
| structure determination of streptococcus suis serotype 14 capsular polysaccharide. | the capsular polysaccharide (cps) of streptococcus suis serotype 14 was purified, chemically modified, and characterized. sugar and absolute configuration analyses gave the following cps composition: d-gal, 3; d-glc, 1; d-glcnac, 1; d-neu5ac, 1. the sambucus nigra lectin, which recognizes the neu5ac(α2-6)gal/galnac sequence, showed binding to the native cps. sialic acid was found to be terminal, and the cps was quantitatively desialylated by mild acid hydrolysis. it was also submitted to perioda ... | 2012 | 23527632 |
| shifts in plant foliar and floral metabolomes in response to the suppression of the associated microbiota. | the phyllospheric microbiota is assumed to play a key role in the metabolism of host plants. its role in determining the epiphytic and internal plant metabolome, however, remains to be investigated. we analyzed the liquid chromatography-mass spectrometry (lc-ms) profiles of the epiphytic and internal metabolomes of the leaves and flowers of sambucus nigra with and without external antibiotic treatment application. | 2016 | 27048394 |
| antibacterial and antiadhesive activities of extracts from edible plants against soft drink spoilage by asaia spp. | this study was conducted to investigate the antibacterial and antiadhesive activities of ethanol extracts from five edible plant parts: cinnamon bark ( cinnamomum zeylanicum ), licorice root ( glycyrrhiza radix ), nettle leaves ( urtica dioica ), green tea leaves ( camellia sinensis ), and elderberry flowers ( sambucus nigra ). the chemical constituents of the extracts were identified using high-performance liquid chromatography and liquid chromatography plus mass spectrometry. six strains of as ... | 2017 | 28221885 |
| detection of expression of influenza virus receptors in tissues of balb/c mice by histochemistry. | infection of host cells with the influenza virus is mediated by specific interactions between the viral hemagglutinin and its cell receptor, oligosaccharides containing sialic acid (sa) residues. avian and human influenza viruses preferentially bind to α-2, 3-linked and α-2, 6-linked sialic acids, respectively. therefore, differential expression of these receptors may be crucial to influenza virus infection. to date, the distribution of these two receptors has never been investigated in the tiss ... | 2009 | 19662506 |
| the effects of sambucus nigra polyphenols on oxidative stress and metabolic disorders in experimental diabetes mellitus. | the paper focuses on certain natural polyphenolic extracts from common elder fruit (sambucus nigra), and also on their effects in diabetes mellitus. the results reveal that the glycosylated hemoglobin values are much higher in the diabetic group and they are significantly lower in the group protected by polyphenols. the natural polyphenol compounds reduce the lipids peroxides, neutralize the lipid peroxil radicals and inhibit the ldl oxidation. following the perturbation of the lipid metabolism ... | 2009 | 20119824 |
| identification of magnolia officinalis l. bark extract as the most potent anti-inflammatory of four plant extracts. | this study was designed to compare the anti-inflammatory potential of a magnolia officinalis l. bark extract solely or in combination with extracts prepared from either polygonum aviculare l., sambucus nigra l., or isodon japonicus l. in bacterial lipopolysaccharide (lps) stimulated human gingival fibroblasts (hgf-1) and human u-937 monocytes, as cell models of periodontal disease. hgf-1 and u-937 cells were incubated with lps from either porphyromonas gingivalis or escherichia coli together wit ... | 2013 | 23711140 |
| screening for bioactive metabolites in plant extracts modulating glucose uptake and fat accumulation. | dichloromethane and methanol extracts of seven different food and medicinal plants were tested in a screening platform for identification of extracts with potential bioactivity related to insulin-dependent glucose uptake and fat accumulation. the screening platform included a series of in vitro bioassays, peroxisome proliferator-activated receptor (ppar) γ-mediated transactivation, adipocyte differentiation of 3t3-l1 cell cultures, and glucose uptake in both 3t3-l1 adipocytes and primary porcine ... | 2014 | 25254050 |
| a fish model for the study of the relationship between neuroendocrine and immune cells in the intestinal epithelium: silurus glanis infected with a tapeworm. | immunohistochemical, immunofluorescence and ultrastructural studies were conducted on a sub-population of 20 wels catfish silurus glanis from a tributary of the river po (northern italy). fish were examined for the presence of ecto- and endo-parasites; in the intestine of 5 fish, 11 specimens of cestode glanitaenia osculata were noted and was the only helminth species encountered. the architecture of intestine and its cellular features were nearly identical in either the uninfected s. glanis or ... | 2017 | 28330806 |
| identification of bioactive compounds from flowers of black elder (sambucus nigra l.) that activate the human peroxisome proliferator-activated receptor (ppar) gamma. | obesity is one of the predisposing factors for the development of overt type 2 diabetes (t2d). t2d is caused by a combination of insulin resistance and beta-cell failure and can be treated with insulin sensitizing drugs that target the nuclear receptor peroxisome proliferator-activated receptor (ppar) gamma. extracts of elderflowers (sambucus nigra) have been found to activate ppargamma and to stimulate insulin-dependent glucose uptake suggesting that they have a potential use in the prevention ... | 2010 | 20222152 |
| tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs. | reports of influenza a virus infections in dogs has received considerable attention from veterinarians, virologists, and epidemiologists. interaction between influenza viral hemagglutinin and cell oligosaccharides containing sialic acid residues results in infection. sialic acids have an α-2,3-linkage to the penultimate galactose in the avian influenza virus receptor and an α-2,6-linkage in the human receptor. to date, there are no detailed data on the tissue distribution or histological feature ... | 2012 | 23000577 |
| a sialylated glycan microarray reveals novel interactions of modified sialic acids with proteins and viruses. | many glycan-binding proteins in animals and pathogens recognize sialic acid or its modified forms, but their molecular recognition is poorly understood. here we describe studies on sialic acid recognition using a novel sialylated glycan microarray containing modified sialic acids presented on different glycan backbones. glycans terminating in beta-linked galactose at the non-reducing end and with an alkylamine-containing fluorophore at the reducing end were sialylated by a one-pot-three-enzyme s ... | 2011 | 21757734 |
| glycomic profile of the human parotid gland between 18th and 26th week of fetal development. | the formation of new and functional structural components of several organs, such as parotid glands, can be influenced by the glycocode. this study analyzed the glycobiology of parotid salivary gland tissue during fetal development using specific biochemical probes (lectins and antibodies). eleven parotid gland samples from human fetuses were obtained from spontaneous abortions at 14-28 weeks of gestation, and tissue sections were analyzed for lectin histochemistry and immunohistochemistry. from ... | 2016 | 27665974 |
| lectin histochemistry shows wga, pha-l and hpa binding increases during progression of human colorectal cancer. | most colorectal carcinomas develop from an adenoma-carcinoma sequence to metastatic disease. the aim of the present study was to use lectins, carbohydrate-binding proteins, to detect changes in glycosylation during this malignant progression. | 2015 | 26408694 |
| anti-influenza virus effects of elderberry juice and its fractions. | elderberry (sambucus nigra l.) has traditionally been used for treating influenza and colds. we evaluated the antiviral effect of concentrated juice of elderberry (cj-e) on the human influenza a virus (ifv). cj-e had a relatively strong effect on ifv-infected mice, although its anti-ifv activity was weak in a cell culture system. the in vivo anti-ifv activities of the fractions were determined after separating cj-e by ultrafiltration and anion-exchange chromatography. oral administration of the ... | 2012 | 22972323 |
| analysis of recombinant duffy protein-linked n-glycans using lectins and glycosidases. | duffy antigen is a glycosylated blood group protein acting as a malarial and chemokine receptor. using glycosylation mutants we have previously demonstrated, that all three potential glycosylation sites of the duffy antigen are occupied by n-linked oligosaccharide chains. in this study, wild-type duffy glycoprotein and three mutants, each containing a single n-glycan, were used to characterize the oligosaccharide chains by lectin blotting and endoglycosidase digestion. the positive reaction of a ... | 2010 | 20234884 |
| combining lectin microcolumns with high-resolution separation techniques for enrichment of glycoproteins and glycopeptides. | silica-based lectin microcolumns are described in this study together with the chemical procedures necessary for their preparation. the analytical merits of canavalia ensiformis and sambucus nigra lectins, [immobilized on activated macroporous silica], such as binding capacity, trapping reproducibility, and substrate selectivity, have been evaluated using model glycoproteins. the described microcolumns are applicable to high-pressure analytical schemes utilizing microvalving procedures, washing ... | 2005 | 15987113 |
| sialic acid residues in the labial salivary glands from sjögren's syndrome patients. | to investigate the composition and expression of sialic acid in the labial salivary glands (lsg) in sjögren's syndrome (ss). | 2017 | 10609070 |
| a lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma. | glycans are cell-type-specific, posttranslational protein modifications that are modulated during developmental and disease processes. as such, glycoproteins are attractive biomarker candidates. here, we describe a mass spectrometry-based workflow that incorporates lectin affinity chromatography to enrich for proteins that carry specific glycan structures. as increases in sialylation and fucosylation are prominent among cancer-associated modifications, we focused on sambucus nigra agglutinin (sn ... | 2011 | 20705048 |
| altered sialylation on the cell-surface proteins of dexamethasone-treated human macrophages contributes to augmented uptake of apoptotic neutrophils. | macrophages eliminate apoptotic granulocytes before their secondary necrosis during resolution of inflammation. a well-known glucocorticoid, the anti-inflammatory dexamethasone augments phagocytosis capacity of macrophages with a so far not fully clarified mechanism. we have found that sialylation of cell-surface proteins on human macrophages is markedly altered by dexamethasone. compared to non-treated cells, dexamethasone-treated macrophages can bind significantly less sambucus nigra lectin sp ... | 2010 | 20937308 |
| redox regulation of morphology, cell stiffness, and lectin-induced aggregation of human platelets. | redox regulation and carbohydrate recognition are potent molecular mechanisms which can contribute to platelet aggregation in response to various stimuli. the purpose of this study is to investigate the relationship between these mechanisms and to examine whether cell surface glycocalyx and cell stiffness of human platelets are sensitive to the redox potential formed by glutathione. to this end, human platelets were treated with different concentrations (0.05 μm to 6 mm) and ratios of reduced or ... | 2010 | 21079947 |
| elucidation of n-glycosites within human plasma glycoproteins for cancer biomarker discovery. | glycans are an important class of post-translational modifications that decorate a wide array of protein substrates. these cell-type specific molecules, which are modulated during developmental and disease processes, are attractive biomarker candidates as biology regarding altered glycosylation can be used to guide the experimental design. the mass spectrometry (ms)-based workflow described here incorporates chromatography on affinity matrices formed from lectins, proteins that bind specific gly ... | 2013 | 23296540 |
| anti-toxoplasma activities of methanolic extract of sambucus nigra (caprifoliaceae) fruits and leaves. | toxoplasma gondii is an obligatory intracellular parasite that infects a wide range of warm-blooded animals and humans. considering the severity of toxoplasmosis, side effects of current treatments, and the contribution of the ethnopharmacological knowledge for the treatment of parasitic infections, the aim of the present study was to investigate the efficacy of methanolic extracts from the fruits and leaves of sambucus nigra against tachyzoite of t gondii. for this, fruits and leaves of s. nigr ... | 2015 | 26299111 |
| sialylation transmogrifies human breast and pancreatic cancer cells into 3d multicellular tumor spheroids using cyclic rgd-peptide induced self-assembly. | multicellular tumor spheroids (mts) have been at the forefront of cancer research, designed to mimic tumor-like developmental patterns in vitro. tumor growth in vivo is highly influenced by aberrant cell surface-specific sialoglycan structures on glycoproteins. aberrant sialoglycan patterns that facilitate mts formation are not well defined. matrix-free spheroids from breast mcf-7 and pancreatic panc1 cancer cell lines and their respective tamoxifen (tmx) and gemcitabine (gem) resistant variants ... | 2016 | 27608845 |
| anti-t haemolysins: the effects of sialic acid removal and 2-aminoethylisothiouronium bromide treatment of erythrocytes on immune lysis. | intravascular haemolytic reactions are reported in red-cell t-activated patients after blood transfusion. the relationship between t antigen antibodies present in normal plasma and these reactions remains unclear. in this study, we assessed the haemolytic activity of t antibodies in vitro in comparison with anti-a/b antibodies. | 2010 | 21118267 |
| characterization of phenolic compounds in flowers of wild medicinal plants from northeastern portugal. | crataegus monogyna, cytisus multiflorus, malva sylvestris and sambucus nigra have been used as important medicinal plants in the iberian peninsula since a long time ago, and are claimed to have various health benefits. this study aimed to determine the phenolic profile and composition of wild medicinal flowers of those species. the analysis was performed by hplc-dad-esi/ms. flavonoids, and particularly flavonols and flavones, were the main groups in almost all the studied samples. c. multiflorus ... | 2012 | 22342808 |
| histochemical localization of glycoconjugates on microglial cells in alzheimer's disease brain samples by using abrus precatorius, maackia amurensis, momordica charantia, and sambucus nigra lectins. | four lectins (abrus precatorius (apa), maackia amurensis (maa), momordica charantia (mca) and sambucus nigra (sna)) have been used to identify glycohistochemically the microglial cells (mgc) activation in autoptic brain samples from alzheimer's disease (ad) subjects. three of these lectins (apa, maa and mca) have utilized as microglial cell markers for the first time. the identification of new markers for the study of mgc is very important to better understand the role of these type of cells in ... | 1998 | 9743580 |
| a cross-cultural comparison of folk plant uses among albanians, bosniaks, gorani and turks living in south kosovo. | kosovo represents a unique hotspot of biological and cultural diversity in europe, which allows for interesting cross-cultural ethnobotanical studies. the aims of this study were twofold: 1) to document the state of traditional knowledge related to local (esp. wild) plant uses for food, medicine, and handicrafts in south kosovo; and 2) to examine how communities of different ethnic groups in the region (albanians, bosniaks/gorani, and turks) relate to and value wild botanical taxa in their ecosy ... | 2015 | 25964167 |
| ribosome-inactivating lectins with polynucleotide:adenosine glycosidase activity. | lectins from aegopodium podagraria (apa), bryonia dioica (bda), galanthus nivalis (gna), iris hybrid (ira) and sambucus nigra (snai), and a new lectin-related protein from sambucus nigra (snlrp) were studied to ascertain whether they had the properties of ribosome-inactivating proteins (rip). ira and snlrp inhibited protein synthesis by a cell-free system and, at much higher concentrations, by cells and had polynucleotide:adenosine glycosidase activity, thus behaving like non-toxic type 2 (two c ... | 1997 | 9188793 |
| potential allelochemicals from sambucus nigra. | twenty-four aromatic metabolites belonging to cyanogenins, lignans, flavonoids, and phenolic glycosides were obtained from sambucus nigra. structures were determined on the basis of their spectroscopic features. two compounds have been isolated and identified as (2s)-2-o-beta-d-glucopyranosyl-2-hydroxyphenylacetic acid and benzyl 2-o-beta-d-glucopyranosyl-2,6-dihydroxybenzoate. all the compounds have been assayed on dicotyledons lactuca sativa (lettuce) and raphanus sativus (radish) and monocoty ... | 2001 | 11730871 |
| the use of common elder sambucus nigra to promote aphidophagous syrphids in apple orchards. | elder shrubs (sambucus nigra l.) were planted in an experimental apple orchard as bordering hedgerow with the objective of rearing syrphids (diptera: syrphidae) and consequently enhancing the biological control of the rosy apple aphid dysaphis plantaginea (passerini). the inoculative introduction of the specific elder aphid aphis sambuci l. (homoptera: aphididae) in late march 2002 and again in 2003 led to the complete hedgerow turning into an early and productive alternative prey reservoir for ... | 2005 | 16628887 |
| herbal medicines as diuretics: a review of the scientific evidence. | there is increasing interest in the health and wellness benefits of herbs and botanicals. this is with good reason as they might offer a natural safeguard against the development of certain conditions and be a putative treatment for some diseases. one such area may be the lowering of blood pressure in those where it is elevated (i.e., hypertension). one class of clinical medicines used to lower blood pressure are known as diuretics and work by increasing the excretion of urine from the body as w ... | 2007 | 17804183 |
| ribosome inactivating protein and lectin from bitter melon (momordica charantia) seeds: sequence comparison with related proteins. | one of the ribosome inactivating proteins (rips), beta-momorcharin, and a lectin were isolated from seeds of the bitter melon momordica charantia (family cucurbitaceae) in accordance with published procedures. both beta-momorcharin and m. charantia lectin were then subjected to amino acid sequencing. beta-momorcharin exhibited considerable homology in sequence to other cucurbitaceae rips, and also to the a chains of abrin and ricin which are type ii (double-chained) rips. the resemblance between ... | 1998 | 9875234 |
| structure and macromolecular composition of the zebrafish egg chorion. | the chorion is the acellular envelope surrounding mature eggs of teleostean fish. the macromolecular composition of the zebrafish (danio rerio) egg chorion, organised as a three-layered structure, has been analysed. sds-page analysis, under reducing conditions, of isolated and purified chorions revealed a reproducible pattern of four major polypeptides (116, 97, 50 and 43 kda) and several minor bands. lectin binding assays showed that both the 116 kda and 50 kda proteins were recognised by conca ... | 1996 | 8913023 |
| altered glycosylation, expression of serum haptoglobin and alpha-1-antitrypsin in chronic hepatitis c, hepatitis c induced liver cirrhosis and hepatocellular carcinoma patients. | liver cirrhosis with hepatitis c viral infection (hcv-lc) causes high risk to develop hepatocellular carcinoma (hcc). besides diagnosis of liver cirrhosis by biochemical test, imaging techniques, assessment of structural liver damage by biopsy shows several disadvantages. our aim was to monitor the changes in the expression level of serum proteins and their glycosylation pattern among chronic hepatitis c (hcv-ch), hcv-lc and hcc patients with respect to controls. 2d gel electrophoresis of hcv-ch ... | 2016 | 27034286 |
| transition rates of selected metals determined in various types of teas (camellia sinensis l. kuntze) and herbal/fruit infusions. | teas and raw materials used as ingredients of herbal and fruit infusions (hfi) were analysed by means of icp-ms for their content of aluminium, arsenic, cadmium, copper, lead and mercury in the dry product and in the infusion. samples of tea (camellia sinensis l. kuntze) were selected to include different origins, types (black, green), leaf grades (whole leaf, broken, fannings, dust) and manufacturing techniques (orthodox, "crush, tear, curl"). the selected hfi raw materials (chamomile, elderber ... | 2017 | 27542446 |
| [changes of alkaline phosphatase sugar chains in hepatocellular carcinoma tissue]. | to investigate the changes of sugar chain structures of alkaline phosphatase (alp) in hepatoma tissue and its relation to the invasiveness of hepatocellular carcinoma (hcc). | 2003 | 14697136 |
| a novel tumor-associated mucin of gastrointestinal carcinoma. | to identify a new tumor-associated antigen, a monoclonal antibody, sc142, was produced by immunizing mice with a stomach cancer cell line. the tumor specificity of mab sc142 was studied by immunohistochemical staining, and the biochemical characteristics of this new gastrointestinal tumor-associated antigen were also studied. | 2001 | 11570576 |
| an observational study and quantification of the actives in a supplement with sambucus nigra and asparagus officinalis used for weight reduction. | the aim of the study was to obtain information on the content of co-active compounds of a food supplement recommended as a weight reduction diet and on its short-term effectiveness and safety as a starter for lifestyle change. eighty participants completed the protocol. the sambucus nigra l. berry juice enriched with flower extract and tablets containing berry powder and flower extract provided a total of 1 mg anthocyanins, 370 mg flavonol glycosides and 150 mg hydroxycinnamates per day; the asp ... | 2008 | 18350516 |
| glycan profiling of endometrial cancers using lectin microarray. | cell surface glycans change during the process of malignant transformation. to characterize and distinguish endometrial cancer and endometrium, we performed glycan profiling using an emerging modern technology, lectin microarray analysis. the three cell lines, two from endometrial cancers [well-differentiated type (g1) and poorly differentiated type (g3)] and one from normal endometrium, were successfully categorized into three independent groups by 45 lectins. furthermore, in cancer cells, a cl ... | 2012 | 22957961 |
| protein-inorganic hybrid nanoflowers as ultrasensitive electrochemical cytosensing interfaces for evaluation of cell surface sialic acid. | the identification of biocompatible nanomaterials with high conductivities as sensing interfaces is important in developing novel electrochemical cytosensors. we prepared a novel protein-inorganic nanomaterial-bovine serum albumin (bsa) incorporated ag nanoflowers with three-dimensional porous architectures, using a simple biomimetic method. the bsa-incorporated ag nanoflowers were modified on a glassy carbon electrode (gce) surface and conjugated with a targeting lectin molecule, i.e., sambucus ... | 2015 | 25599845 |
| construction and validation of a sambucus nigra biosensor for cancer-associated stn antigen. | a label-free electrochemical impedance spectroscopy biosensor for selective detection and discrimination of the cancer-associated sialyl-tn (stn) antigen was developed by using sambucus nigra agglutinin type i (sna-i) as the recognition element. the sna-i biosensor was constructed by immobilizing the lectin on screen-printed gold electrodes. the formation of a complex between sna-i and stn-containing glycoproteins (transferrin and bovine submaxillary mucin) was monitored by measuring the impedan ... | 2014 | 24594592 |
| the presence of n-acetylneuraminic acid in malpighian tubules of larvae of the cicada philaenus spumarius. | sialic acid-containing glycoconjugates are generally considered to be unique to the deuterostomes, a lineage of the animal kingdom which includes animals from the echinoderms up to the vertebrates. there are, however, two isolated reports of sialic acid occurring in the insect species drosophila melanogaster and galleria mellonella. since insects are classified as protostomes, these findings call previous assumption on the phylogenetic distribution and thus on the evolution of sialic acids into ... | 1999 | 11003558 |
| traditional food and herbal uses of wild plants in the ancient south-slavic diaspora of mundimitar/montemitro (southern italy). | | 2012 | 22672636 |
| role of glycosylation in the anticancer activity of antibacterial peptides against breast cancer cells. | antibacterial peptides (abps) with cancer-selective toxicity have received much more attention as alternative chemotherapeutic agents in recent years. however, the basis of their anticancer activity remains unclear. the modification of cell surface glycosylation is a characteristic of cancer cells. the present study investigated the effect of glycosylation, in particular sialic acid, on the anticancer activity of abps. we showed that aurein 1.2, buforin iib and bmap-28m exhibited selective cytot ... | 2013 | 23962446 |
| apoptotic cells selectively uptake minor glycoforms of vitronectin from serum. | apoptosis profoundly alters the carbohydrate layer coating the membrane of eukaryotic cells. previously we showed that apoptotic cells became reactive with the α2,6-sialyl-specific lectin from sambucus nigra agglutinin (sna), regardless of their histological origin and the nature of the apoptotic stimulus. here we reveal the basis of the phenomenon by showing that in apoptotic cancer cell lines sna reactivity was mainly associated with a 67 kda glycoprotein which we identified by maldi-tof/tof a ... | 2013 | 23381642 |
| anti-cytokine therapy for prevention of atherosclerosis. | currently a chronic inflammation is considered to be the one of the most important reasons of the atherosclerosis progression. a huge amount of researches over the past few decades are devoted to study the various mechanisms of inflammation in the development of atherosclerotic lesions. | 2016 | 26781385 |
| separation of flavonol-2-o-glycosides from calendula officinalis and sambucus nigra by high-performance liquid and micellar electrokinetic capillary chromatography. | calendula officinalis and sambucus nigra flowers were analysed by reversed-phase high-performance liquid chromatography (rp-hplc) and micellar electrokinetic capillary chromatography (mecc). rp-hplc was performed on c8 aquapore rp 300 columns with eluents containing 2-propanol and tetrahydrofuran. mecc was carried out on a 72-cm fused-silica capillary using sodium dodecyl sulphate and sodium borate (ph 8.3) as the running buffer. the results obtained by these techniques are compared. | 1992 | 1639901 |
| a lectin microarray study of glycoantigens in neonatal porcine islet-like cell clusters. | besides α-gal expression, the differences of glycosylation and antigenicity between adult pig islets (apis) and neonatal porcine islet-like cell clusters (npccs) are altogether unclear. in this study, lectin microarray analyses of npccs were performed and the results compared with the corresponding values for wild-type apis and npccs from α-gal transferase knockout (galt-ko) pig. | 2013 | 23769020 |
| blot analysis with lectins for the evaluation of glycoproteins in cultured cells and tissues. | the carbohydrate-binding properties of plant and animal lectins have found many diverse applications in biomedical research that are comprehensively treated in the various chapters of this book. in our studies, lectins are often applied for the in situ detection of their respective binding sites in specific cells either grown in culture or present in various tissues (see chapter 3 ). in order to complement these in situ localization studies, blots are analyzed using lectins to identify glycosyla ... | 1998 | 21374457 |
| lectins selectively label cartilage condensations and the otic neuroepithelium within the embryonic chicken head. | cartilage morphogenesis during endochondral ossification follows a progression of conserved developmental events. cells are specified towards a prechondrogenic fate and subsequently undergo condensation followed by overt differentiation. currently available molecular markers of prechondrogenic and condensing mesenchyme rely on common regulators of the chondrogenic program that are not specific to the tissue type or location. therefore tissue-specific condensations cannot be distinguished based o ... | 2017 | 27861854 |
| model of human recurrent respiratory papilloma on chicken embryo chorioallantoic membrane for tumor angiogenesis research. | we aimed to develop a chick embryo chorioallantoic membrane (cam) model of recurrent respiratory papilloma (rpp) and to evaluate its morphological and morphometric characteristics, together with angiogenic features. fresh rrp tissue samples obtained from 13 patients were implanted in 174 chick embryo cams. morphological, morphometric, and angiogenic changes in the cam and chorionic epithelium were evaluated up until 7 days after the implantation. immunohistochemical analysis (34βe12, ki-67, mmp- ... | 2016 | 27723025 |
| electrochemical detection of glycan and protein epitopes of glycoproteins in serum. | aberrant protein glycosylation is associated with a range of pathological conditions including cancer and possesses diagnostic importance. translation of glycoprotein biomarkers will be facilitated by the development of a rapid and sensitive analytical platform that simultaneously interrogates both the glycan and protein epitopes of glycoproteins in body fluids such as serum or saliva. to this end, we developed an electrochemical biosensor based on the immobilization of a lectin on the gold elec ... | 2014 | 25267970 |
| differential lectin recognition of glycoproteins in choanomastigote-shaped trypanosomatids: taxonomic implications. | the glycoprotein profiles of seven choanomastigote-shaped trypanosomatids (six crithidia spp. and one herpetomonas sp.), which have been suggested to form three distinct taxonomic groups (crithidia, angomonas and strigomonas), were analyzed by western blotting using the lectins limax flavus (lfa), sambucus nigra (sna) and maackia amurensis (maa), which specifically recognize sialic acid residues, and concanavalin a (con a) that recognizes mannose-like residues in glycoconjugates. all lectins sho ... | 2004 | 14987761 |
| influence of altitudinal variation on the content of phenolic compounds in wild populations of calluna vulgaris, sambucus nigra, and vaccinium myrtillus. | this study deals with the effect of altitudinal variation on the content of phenolic compounds in three traditional herbal plants, which are also consumed as food in central europe. herbs of calluna vulgaris (l.) hull, flowers and fruits of sambucus nigra l., and berries of vaccinium myrtillus l. collected in the naturpark solktaler (austria) were extracted using accelerated solvent extraction (ase). identification and quantification of the constituents in the polar extracts (methanol 80%, v/v) ... | 2008 | 18788745 |
| posttranslational modifications of the insulin-like growth factor-binding protein 3 in patients with type 2 diabetes mellitus assessed by affinity chromatography. | structural and ligand-binding properties of the insulin-like growth factor-binding protein (igfbp)-3 in patients with poorly controlled diabetes mellitus type 2 were investigated using boronic acid- and lectin-affinity chromatography. igfbp-3 species separated by chromatography were analyzed by immunoblotting and surface-enhanced laser desorption/ionization-time of flight mass spectrometry (seldi-tof ms). increased igfbp-3 binding to boronic acid in patients was shown to be accompanied by the in ... | 2012 | 22884472 |
| glycoproteins from the cuticle of the atlantic shore crab carcinus maenas: i. electrophoresis and western-blot analysis by use of lectins. | the protein and glycoprotein content of four different neutral or acidic solvent extracts (0.5 m kcl, 10% edta, 0.1 n hcl, or 2% acetic acid) from the mineralized exoskeleton of a decapod crustacean, the atlantic shore crab carcinus maenas, were characterized by quantitative analysis of proteins, sds-page analysis, and probing with lectins on blots. the lectins used were conconavalin a, jacalin, soybean agglutinin, maackia amurensis agglutinin ii, and sambucus nigra agglutinin. the results show ... | 2002 | 11842016 |
| nutrient and energy content, in vitro ruminal fermentation characteristics and methanogenic potential of alpine forage plant species during early summer. | plants growing on alpine meadows are reported to be rich in phenols. such compounds may affect ruminal fermentation and reduce the plants' methanogenic potential, making alpine grazing advantageous in this respect. the objective of this study was to quantify nutrients and phenols in alpine forage grasses, herbs and trees collected over 2 years and, in a 24 h in vitro incubation, their effects on ruminal fermentation parameters. | 2011 | 21480269 |
| particulate matter on foliage of 13 woody species: deposition on surfaces and phytostabilisation in waxes--a 3-year study. | particulate matter (pm) as an air pollutant can be harmful for human health through allergic, mutagenic and carcinogenic effects. although the main focus is on decreasing air pollution, after pm has been emitted to the atmosphere, one ofthe realistic options to decrease it's concentrations in urbanized area will be phytoremediation. this study compared the capacity to capture pm from air of seven tree species commonly cultivated in poland (catalpa bignonioides walter, corylus colurna l., fraxinu ... | 2013 | 23488010 |
| a molecular docking study of phytochemical estrogen mimics from dietary herbal supplements. | the purpose of this study is to use a molecular docking approach to identify potential estrogen mimics or anti-estrogens in phytochemicals found in popular dietary herbal supplements. | 2015 | 25878948 |
| fifteen-year phenological plant species and meteorological trends in central italy. | the present study was carried out in a phenological garden in central italy that contains vegetative clones of shrubs and trees common to several international phenological gardens, such as cornus sanguinea l.; corylus avellana l.; ligustrum vulgare l.; robinia pseudoacacia l.; salix acutifolia willd. and sambucus nigra l. vegetative plant growth monitoring was carried out weekly using common international keys: bbch07, bud break and leaf unfolding; bbch19, young unfolded leaf; bbch91, adult lea ... | 2014 | 23435513 |
| link between defoliation and light treatments on root vitality of five understory shrubs with different resistance to insect herbivory. | understory shrubs are frequently attacked by insect herbivores. however, very little is known regarding possible interactions between light condition, defoliation (d) and fine root vitality (% live roots) and metabolic activity, and whether different plant strategies (compensation, trade-off and equilibrium) to defoliation depend on individual species light requirements. to explore the response of roots to such conditions, an experiment was established in which we experimentally removed 50% of l ... | 2010 | 20624813 |
| assessment of extracts of helichrysum arenarium, crataegus monogyna, sambucus nigra in photoprotective uva and uvb; photostability in cosmetic emulsions. | the aim of our study was to investigate the photoprotective activity and photostability efficacy of sunscreen formulations containing helichrysum arenarium, sambucus nigra, crataegus monogyna extracts and their combination. uv transmission of the emulsion films was performed by using diffuse transmittance measurements coupling to an integrating sphere. in vitro photoprotection and photostability efficacy were evaluated according to the following parameters: sun protection factor (spf), uva prote ... | 2013 | 24007865 |
| both α2,3- and α2,6-linked sialic acids on o-linked glycoproteins act as functional receptors for porcine sapovirus. | sapovirus, a member of the caliciviridae family, is an important cause of acute gastroenteritis in humans and pigs. currently, the porcine sapovirus (psav) cowden strain remains the only cultivable member of the sapovirus genus. while some caliciviruses are known to utilize carbohydrate receptors for entry and infection, a functional receptor for sapovirus is unknown. to characterize the functional receptor of the cowden strain of psav, we undertook a comprehensive series of protein-ligand bioch ... | 2014 | 24901849 |
| production of α2,6-sialylated igg1 in cho cells. | the presence of α2,6-sialic acids on the fc n-glycan provides anti-inflammatory properties to the iggs through a mechanism that remains unclear. fc-sialylated iggs are rare in humans as well as in industrial host cell lines such as chinese hamster ovary (cho) cells. facilitated access to well-characterized α2,6-sialylated iggs would help elucidate the mechanism of this intriguing igg's effector function. this study presents a method for the efficient fc glycan α2,6-sialylation of a wild-type and ... | 2015 | 25875452 |
| chinese hamster ovary (cho) host cell engineering to increase sialylation of recombinant therapeutic proteins by modulating sialyltransferase expression. | n-glycans of human proteins possess both α2,6- and α2,3-linked terminal sialic acid (sa). recombinant glycoproteins produced in chinese hamster overy (cho) only have α2,3-linkage due to the absence of α2,6-sialyltransferase (st6gal1) expression. the chinese hamster st6gal1 was successfully overexpressed using a plasmid expression vector in three recombinant immunoglobulin g (igg)-producing cho cell lines. the stably transfected cell lines were enriched for st6gal1 overexpression using fitc-sambu ... | 2017 | 25641927 |
| influence of sambucus nigra bark lectin on cell dna under different in vitro conditions. | the biological activity of sambucus nigra bark lectin on chinese hamster cells in vitro was investigated by comet-assay and cytotoxicity testing. mitogenic properties at the concentrations 0.063-0.25 microg/ml (but not higher) were found, and the induction of dna breaks at concentrations 0.5 microg/ml and higher is demonstrated. s. nigra bark lectin at mitogenic concentrations decreased the level of nickel-induced dna damage. the character and mechanism of this lectin protective activity was pro ... | 2005 | 15763496 |
| localization and identification of galactose/n-acetylgalactosamine and sialic acid-containing proteins in chinese hamster metaphase chromosomes. | four lectins were used to recognize galactose/n-acetyl-galactosamine (gal/galnac) and sialic acid residues in proteins of chinese hamster metaphase chromosomes. in situ binding pattern of a fluorescein isothiocyanate-labelled (gal/galnac)-specific lectin sophora japonica agglutinin (sja) showed that chromosomal sja-binding proteins are primarily localized to the helically coiled substructure of chromatids. numerous sja-binding proteins were identified in western blots of chromosomal proteins, th ... | 1998 | 9878094 |
| p-selectin and e-selectin. distinct but overlapping leukocyte ligand specificities. | p-selectin on platelets and endothelial cells and e-selectin on endothelial cells are leukocyte receptors that recognize lineage-specific carbohydrates on neutrophils and monocytes. the proposed ligands for these receptors contain the le(x) core and sialic acid. since other investigators have shown that both e-selectin and p-selectin bind to sialylated le(x), we evaluated whether e-selectin and p-selectin recognize the same counter-receptor on leukocytes. the interaction of hl60 cells with chine ... | 1992 | 1375936 |
| changes of glycosylation of igg in rheumatoid arthritis patients treated with methotrexate. | in patients with active rheumatoid arthritis (ra) decrease of galactosylation is correlated with disease activity. the aim of our study was to evaluate an effect of methotrexate therapy on glycosylation disturbances of igg in ra patients. | 2016 | 26876088 |
| identification of 45 kd antigen in immune complexes of patients of allergic bronchopulmonary aspergillosis. | a glycoprotein antigen with an apparent mw of 45 kd was observed to be predominant in the circulating immune complexes isolated from patients of allergic bronchopulmonary aspergillosis as well as in the immune complexes prepared in vitro. further characterisation of 45 kd antigen with trypsin showed four immunologically active peptides with mw 43, 36, 33 and 16 kd. carbohydrate characterization using various lectins (maackia amurensis agglutinin, sambucus nigra agglutinin, peanut agglutinin, gal ... | 1997 | 9046027 |
| alpha-d-galactose-bearing glycoproteins on the surface of stimulated murine peritoneal macrophages. biochemical and immunochemical characterization of purified glycoproteins. | two glycoproteins were isolated from lysates of thioglycollate-stimulated, murine peritoneal macrophages by affinity chromatography on immobilized griffonia simplicifolia i lectin and by preparative sds/page. the glycoproteins were readily labeled on the surface of intact macrophages with 3h and 125i. the labeled glycoproteins migrated as broad bands of molecular mass 92-109 kda and 115-125 kda. the mobility of the glycoproteins decreased only slightly after reduction with dithiothreitol, indica ... | 1992 | 1587269 |
| authentication of punica granatum l.: development of scar markers for the detection of 10 fruits potentially used in economically motivated adulteration. | the large commercial success of pomegranate increase the likelihood of economically motivated adulteration (ema), which has been gradually spotted with the undeclared addition of anthocyanin-rich plants or cheaper fruit juices used as bulking and diluting agents. a method based on sequence-characterized amplified regions (scars) was developed to detect the presence of aristotelia chilensis, aronia melanocarpa, dioscorea alata, euterpe oleracea, malus×domestica, morus nigra, sambucus nigra, vacci ... | 2016 | 26920316 |
| identification of n-glycosylated proteins from the central nervous system of drosophila melanogaster. | although the function of many glycoproteins in the nervous system of fruit flies is well understood, information about the glycosylation profile and glycan attachment sites for such proteins is scarce. in order to fill this gap and to facilitate the analysis of n-linked glycosylation in the nervous system, we have performed an extensive survey of membrane-associated glycoproteins and their n-glycosylation sites isolated from the adult drosophila brain. following subcellular fractionation and try ... | 2007 | 17893096 |
| in vitro inhibition of cyp3a4 by the multiherbal commercial product sambucus force and its main constituents echinacea purpurea and sambucus nigra. | the multiherbal product sambucus force contains echinacea purpurea and sambucus nigra as its main constituents. the aims of this study were to evaluate sambucus force's inhibition potential and inhibition mechanisms towards cyp3a4, and to evaluate the inhibitory co-contribution of e. purpurea and s. nigra. metabolic studies were performed with recombinant human cyp3a4, with testosterone as substrate. sambucus force inhibited cyp3a4 activity with a mean (95% confidence interval) half maximal inhi ... | 2012 | 22319006 |
| determination of elements by atomic absorption spectrometry in medicinal plants employed to alleviate common cold symptoms. | eleven important medicinal plants generally used by the people of turkey for the treatment of common cold have been studied for their mineral contents. eleven minor and major elements (essential, non-essential and toxic) were identified in the asplenium adiantum-nigrum l. , althaea officinalis l. , verbascum phlomoides l., euphorbia chamaesyce l., zizyphus jujube miller, peganum harmala l., arum dioscoridis sm., sambucus nigra l., piperlongum l., tussilago farfara l. and elettaria cardamomum mat ... | 2014 | 25532362 |