| characterization of bacterial isolates from industrial wastewater according to probable modes of hexadecane uptake. | bacterial isolates from industrial wastewater were characterized according to probable modes of hexadecane uptake based on data for cell surface hydrophobicity, emulsifying activity, glycoside content and surface tension of cell-free culture medium. the results obtained suggested that both modes of biosurfactant-enhanced hexadecane uptake by bacterial strains take place, direct uptake and alkane transfer. the increase in cell surface hydrophobicity and glycoside production by the strains suggest ... | 2008 | 16962302 |
| adhesion of an amylolytic arthrobacter sp. to starch-containing plastic films. | cells of the amylolytic bacterium kb-1 (thought to be an arthrobacter sp.) adhered ( approximately 70%) to the surface of plastic films composed of starch-poly (methylacrylate) graft copolymer (starch-pma), but did not adhere (<10%) to films composed of polymethylacrylate (pma), polyethylene (pe), carboxymethyl cellulose, or a mixture of pe plus poly (ethylene-coacrylic acid) (eaa), starch plus pe, or starch plus pe and eaa. about 30% of the cells adhered to gelatinized insoluble starch. dithiot ... | 1990 | 16348173 |
| biodegradation by an arthrobacter species of hydrocarbons partitioned into an organic solvent. | an arthrobacter strain mineralized naphthalene and n-hexadecane dissolved in 2,2,4,4,6,8,8-heptamethylnonane. the extent of mineralization increased with greater volumes of solvent. measurements under aseptic conditions of the partitioning of naphthalene into the aqueous phase from the solid phase or from heptamethylnonane showed that the rates were rapid and did not limit mineralization. the rate of mineralization of hexadecane was rapid, although partitioning of the compound into aqueous solut ... | 1991 | 16348485 |
| phenanthrene degradation in arthrobacter sp. p1-1: initial 1,2-, 3,4- and 9,10-dioxygenation, and meta- and ortho-cleavages of naphthalene-1,2-diol after its formation from naphthalene-1,2-dicarboxylic acid and hydroxyl naphthoic acids. | arthrobacter sp. p1-1, isolated from a polycyclic aromatic hydrocarbon (pah)-contaminated site in hilo, hi, usa, can decompose phenanthrene (40 mg l(-1)) completely within 7 days. a detailed phenanthrene metabolism map was constructed based on metabolite analysis and replacement cultures. initial dioxygenation occurs on 1,2-, 3,4-, and 9,10-c of phenanthrene, dominantly on 3,4-c positions. rapid accumulation of 5,6- and 7,8-benzocoumarin suggests that phenanthrene-1,2- and -3,4-diols mainly unde ... | 2006 | 16777186 |
| cloning and nucleotide sequence of carbaryl hydrolase gene (caha) from arthrobacter sp. rc100. | a carbaryl hydrolase gene (caha) encoded on the plasmid prc1 in arthrobacter sp. rc100 was cloned and sequenced. the entire region of the deduced amino acid sequence was found to be homologous to that of an amidase family. parts of the consensus sequences of the amidase gene have been identified in caha from strain rc100. caha was overexpressed in escherichia coli jm109, and the enzyme was purified to homogeneity by protamine sulfate treatment, ammonium sulfate precipitation, and hydrophobic and ... | 2006 | 16781470 |
| [bacterial communities of brown and red algae from peter the great bay, the sea of japan]. | the structure of microbial communities of brown algae, red algae, and of the red alga gracilaria verrucosa, healthy and affected with rotten thallus, were comparatively investigated; 61 strains of heterotrophic bacteria were isolated and characterized. most of them were identified to the genus level, some vibrio spp., to the species level according to their phenotypic properties and the fatty acid composition of cellular lipids. the composition of the microflora of two species of brown algae was ... | 2006 | 16871810 |
| microbial production of optically active beta-phenylalanine through stereoselective degradation of racemic beta-phenylalanine. | the ability to produce (r)- or (s)-beta-phenylalanine from racemic beta-phenylalanine through stereoselective degradation was screened for. variovorax sp. jh2 and arthrobacter sp. the faculty of agriculture, kyoto university (aku) 638 were found to be potential catalysts for (r)- and (s)-beta-phenylalanine production respectively. on 192 h cultivation of variovorax sp. in medium containing 1.0% (w/v) racemic beta-phenylalanine, 0.46% (w/v) (r)-beta-phenylalanine with an enantiomeric purity of 99 ... | 2006 | 16880592 |
| degradation of 4-fluorophenol by arthrobacter sp. strain if1. | a gram-positive bacterial strain capable of aerobic biodegradation of 4-fluorophenol (4-fp) as the sole source of carbon and energy was isolated by selective enrichment from soil samples collected near an industrial site. the organism, designated strain if1, was identified as a member of the genus arthrobacter on the basis of 16s ribosomal rna gene sequence analysis. arthrobacter strain if1 was able to mineralize 4-fp up to concentrations of 5 mm in batch culture. stoichiometric release of fluor ... | 2008 | 18228015 |
| novel chromium tolerant microorganisms: isolation, characterization and their biosorption capacity. | effluent from tannery, electroplating and electronic industries contain chromium which is highly toxic. this paper reports isolation of chromium tolerant microorganisms from solid waste as well as liquid effluent of an electroplating industry. nine isolates were obtained that can tolerate chromium concentration up to 700mg/l. they reach their stationary phase within 8-12h and can biosorb 95% of initial 200mg/l concentration of chromium within 4-10h. fourier transform infra red analysis of the bi ... | 2008 | 18272220 |
| arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity. | arthrobacter sp. lipase (abl, mtcc no. 5125) is being recognized as an efficient enzyme for the resolution of drugs and their intermediates. the immobilization of abl on various matrices for its enantioselectivity, stability, and reusability has been studied. immobilization by covalent bonding on sepharose and silica afforded a maximum of 380 and 40 iu/g activity, respectively, whereas sol-gel entrapment provided a maximum of 150 iu/g activity in dry powder. the immobilized enzyme displayed exce ... | 2006 | 16896604 |
| complete degradation of butyl benzyl phthalate by a defined bacterial consortium: role of individual isolates in the assimilation pathway. | two bacterial strains, in consortium, were isolated by enrichment techniques from municipal waste-contaminated soil, which utilized butyl benzyl phthalate (bbp) as the sole carbon source. one of the isolates was identified as arthrobacter sp. strain wy and the other one as acinetobacter sp. strain fw based on the morphological, nutritional and biochemical characteristics and 16s rrna sequence analysis. various metabolites of bbp engendered by arthrobacter sp. strain wy were isolated and identifi ... | 2008 | 17669462 |
| catalytic mechanism of inulinase from arthrobacter sp. s37. | detailed catalytic roles of the conserved glu323, asp460, and glu519 of arthrobacter sp. s37 inulinase (enia), a member of the glycoside hydrolase family 32, were investigated by site-directed mutagenesis and ph-dependence studies of the enzyme efficiency and homology modeling were carried out for enia and for d460e mutant. the enzyme efficiency (k(cat)/k(m)) of the e323a and e519a mutants was significantly lower than that of the wild-type due to a substantial decrease in k(cat), but not due to ... | 2008 | 18395004 |
| mineralization of diuron [3-(3,4-dichlorophenyl)-1, 1-dimethylurea] by co-immobilized arthrobacter sp. and delftia acidovorans. | mineralization of diuron has not been previously demonstrated despite the availability of some bacteria to degrade diuron into 3,4-dichloroaniline (3,4-dca) and others that can mineralize 3,4-dca. a bacterial co-culture of arthrobacter sp. n4 and delftia acidovorans w34, which respectively degraded diuron (20 mg l(-1)) to 3,4-dca and mineralized 3,4-dca, were able to mineralize diuron. total diuron mineralization (20 mg l(-1)) was achieved with free cells in co-culture. when the bacteria were im ... | 2007 | 17333465 |
| solar-based detoxification of phenol and p-nitrophenol by sequential tio2 photocatalysis and photosynthetically aerated biological treatment. | simulated solar uv/tio(2) photocatalysis was efficient to detoxify a mixture of 100 mgphenoll(-1) and 50 mgp-nitrophenol (pnp) l(-1) and allow the subsequent biodegradation of the remaining pollutants and their photocatalytic products under photosynthetic aeration with chlorella vulgaris. photocatalytic degradation of phenol and pnp was well described by pseudo-first order kinetics (r(2)>0.98) with removal rate constants of 1.9x10(-4) and 2.8x10(-4)min(-1), respectively, when the pollutants were ... | 2007 | 17350074 |
| the characteristics of rhizosphere microbes associated with plants in arsenic-contaminated soils from cattle dip sites. | soil microorganisms and plants were studied in samples of arsenic-contaminated soil from two cattle dip sites. the aim was to delineate the parameters that will determine the feasibility of future remediation by growing arsenic-accumulating plants, including the identity and characteristics of some rhizosphere soil microbes. the soil samples contained high total, but low soluble arsenic concentrations which, together with other properties, resembled the previously reported characteristics of dip ... | 2007 | 17407787 |
| continuous deodorization and bacterial community analysis of a biofilter treating nitrogen-containing gases from swine waste storage pits. | a biofilter inoculated with arthrobacter sp. was applied to the simultaneous elimination of trimethylamine (tma) and ammonia (nh3) from the exhaust air of swine waste storage pits. the results showed that the biofilter achieved average removal efficiencies of 96.8+/-2.5% and 97.2+/-2.3% for tma and nh3, respectively. a near-neutral ph (7.3-7.4) was maintained due to the accumulation of acid metabolites and the adsorption of alkaline nh3. low moisture demand, low pressure drop and high biofilm st ... | 2008 | 17697773 |
| aerobic uranium (vi) bioprecipitation by metal-resistant bacteria isolated from radionuclide- and metal-contaminated subsurface soils. | in this study, the immobilization of toxic uranium [u(vi)] mediated by the intrinsic phosphatase activities of naturally occurring bacteria isolated from contaminated subsurface soils was examined. the phosphatase phenotypes of strains belonging to the genera, arthrobacter, bacillus and rahnella, previously isolated from subsurface soils at the us department of energy's (doe) oak ridge field research center (orfrc), were determined. the orfrc represents a unique, extreme environment consisting o ... | 2007 | 17991039 |
| bacterial endophytes contribute to abiotic stress adaptation in pepper plants (capsicum annuum l.). | endophytes are nonpathogenic plant-associated bacteria that can play an important role in plant vitality and may confer resistance to abiotic or biotic stress. the effects of 5 endophytic bacterial strains isolated from pepper plants showing 1-aminocyclopropane-1-carboxylate deaminase activity were studied in sweet pepper under in vitro conditions. four of the strains tested showed production of indole acetic acid. plant growth, osmotic potential, free proline content, and gene expression were m ... | 2007 | 18026213 |
| simultaneous mineralization of glyphosate and diuron by a consortium of three bacteria as free- and/or immobilized-cells formulations. | a bacterial consortium able to mineralize two herbicides, glyphosate (pseudomonas 4asw) and diuron (arthrobacter sp. n4 and delftia acidovorans), was cultivated in both a synthetic culture medium without phosphate and a sediment extract medium. in the aim at optimizing glyphosate and diuron mineralization, all the combinations, i.e., free and/or immobilized cells in ca-alginate beads were tested. with the synthetic medium, the simultaneous mineralization of glyphosate and diuron required at leas ... | 2008 | 18026726 |
| application of 16s rdna-dgge and plate culture to characterization of bacterial communities associated with the sawfly, acantholyda erythrocephala (hymenoptera, pamphiliidae). | culture-based analysis was employed in parallel with pcr amplification of 16s rdna, coupled with denaturing gradient gel electrophoresis (dgge), to profile bacterial species associated with different developmental stages of the pine false webworm (pfw), acantholyda erythrocephala, a sawfly pest responsible for incidents of severe defoliation in commercially important tree plantations in north america. culture-based analysis revealed that pseudomonas spp. along with bacillus sphaericus and arthro ... | 2008 | 18769850 |
| contribution of ethylamine degrading bacteria to atrazine degradation in soils. | bacterial communities that cooperatively degrade atrazine commonly consist of diverse species in which the genes for atrazine dechlorination and dealkylation are variously distributed among different species. normally, the first step in degradation of atrazine involves dechlorination mediated by atza, followed by stepwise dealkylation to yield either n-ethylammelide or n-isopropylammelide. as the liberated alkylamine moieties are constituents of many organic molecules other than atrazine, it is ... | 2006 | 17064268 |
| crystallization and x-ray diffraction analysis of 6-aminohexanoate-cyclic-dimer hydrolase from arthrobacter sp. ki72. | 6-aminohexanoate-cyclic-dimer hydrolase (ei) from arthrobacter sp. ki72 was expressed in escherichia coli and purified by anion-exchange chromatography. ei was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as precipitant in imidazole buffer ph 8.0. the crystal is hexagonal, with unit-cell parameters a = b = 130.75, c = 58.23 a. diffraction data were collected from native and mercury(ii) dichloride-derivative crystals to resolutions of 1.90 and 2.06 a, respectively. | 2006 | 17142898 |
| amperometric biosensor for the determination of creatine. | an amperometric biosensor for the determination of creatine was developed. the carbon rod electrode surface was coated with sarcosine oxidase (sox) and creatine amidinohydrolase by cross-linking under glutaraldehyde vapour. the sox from arthrobacter sp. 1-1 n was purified and previously used for creation of a creatine biosensor. the natural sox electron acceptor, oxygen, was replaced by an [fe(cn)(6)](3-) /[ fe(cn)(6)](4-) redox mediating system, which allowed amperometric detection of an analyt ... | 2007 | 17221239 |
| coryneform bacteria in semen of chronic prostatitis patients. | our aim was to compare the presence and species composition of coryneform bacteria in chronic prostatitis patients and controls. semen of 50 men with inflammatory prostatitis and 59 controls (without pelvic pain/discomfort complaints and leukocytospermia) was investigated. first-catch urine was additionally investigated in 36 men (30 with and 6 without prostatitis). coryneform bacteria were found in semen of 76% men with inflammatory prostatitis and 83% controls. more than half of the isolates w ... | 2007 | 17163953 |
| [isolation, degradation and phylogenetic analysis of methylparathion degradative strain x4]. | the strain x4 was isolated from polluted wastes of shandong huayang pesticide chemical industry group co., ltd. it was cultured with methylparathion and p-nitrophenol, a kind of intermediate during methylparathion degrading process, and used them as the sole carbon source and decomposed them into carbon dioxide and wastes. it is identified as arthrobacter sp. by means of a series of tests, and it has visible sphere-pole variable cycle. it is bacilliform when it is cultured in nutrient broth medi ... | 2006 | 17302165 |
| arthrobacter sp. lipase immobilization for preparation of enantiopure masked beta-amino alcohols. | recent reports on immobilization of lipase from arthrobacter sp. (abl, mtcc 5125; iiim isolate) on insoluble polymers have shown altered properties including stability and enantioselectivity. present work demonstrates a facile method for the preparation of enantiopure beta-amino alcohols by modulation of abl enzyme properties via immobilization on insoluble as well as soluble supports using entrapment/covalent binding techniques. efficacies of immobilized abl on insoluble supports prepared from ... | 2009 | 19081255 |
| [study on the atrazine-degrading genes in arthrobacter sp. ag1]. | atrazine could be used as the sole carbon, nitrogen and energy sources for growth by strain arthrobacter sp. ag1, and the atrazine-degrading genes of ag1 were found to be the combination of trzn, atzb and atzc. the atrazine chloride hydrolysase gene trzn was cloned by pcr amplification,whose sequence shared 99% identity with that of norcardioides sp. c190. two large plasmids were found in ag1,and trzn and atzb were confirmed to be localized on the larger plasmid pag1 by the method of southern hy ... | 2007 | 18051853 |
| [screening and function analysis of a cyclohexanone-degrading bacterium cn1 from deep sea sediment]. | micrococcu luteus cn1 was found to be able to utilize cyclohexanone well from the strains originally isolated from pacific ocean sediment. the optimum conditions for its growth were determined as 25 degrees c -37 degrees c, ph 8, salinity 6%. it could survive in the medium with high concentration of cyclohexanone ( > 44% v/v), and grew most vigorously in medium with 16.7% (v/v) cyclohexanone. cn1 could transform cyclohexanol to cyclohexanone which could be further degraded and mineralized quickl ... | 2007 | 18062257 |
| [reaction of microorganisms to the digestive fluid of the earthworms]. | the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ... | 2007 | 17410875 |
| structure and denaturation of 4-chlorobenzoyl coenzyme a dehalogenase from arthrobacter sp. strain tm-1. | the secondary structure of the trimeric protein 4-chlorobenzoyl coenzyme a dehalogenase from arthrobacter sp. strain tm-1, the second of three enzymes involved in the dechlorination of 4-chlorobenzoate to form 4-hydroxybenzoate, has been examined. e(mm) for the enzyme was 12.59. analysis by circular dichroism spectrometry in the far uv indicated that 4-chlorobenzoyl coenzyme a dehalogenase was composed mostly of alpha-helix (56%) with lesser amounts of random coil (21%), beta-turn (13%) and beta ... | 2008 | 17431803 |
| cloning and characterization of the gene encoding endo-beta-1,3-glucanase from arthrobacter sp. nhb-10. | the glua gene, encoding an endo-beta-1,3-glucanase from arthrobacter sp. (strain nhb-10), was cloned and analyzed. the deduced endo-beta-1,3-glucanase amino acid sequence was 750 amino acids long and contained a 42 amino acid signal peptide with a mature protein of 708 amino acids. there was no similarity to known endo-beta-1,3-glucanases, but glua was partially similar to two fungal exo-beta-1,3-glucanases in glycoside hydrolase (gh) family 55. of five possible residues for catalysis and two mo ... | 2007 | 17587693 |
| biodegradation and bioremediation of endosulfan contaminated soil. | among the three mixed bacterial culture ae, be, and ce, developed by enrichment technique with endosulfan as sole carbon source, consortium ce was found to be the most efficient with 72% and 87% degradation of alpha-endosulfan and beta-endosulfan, respectively, in 20 days. in soil microcosm, consortium ae, be and ce degraded alpha-endosulfan by 57%, 88% and 91%, respectively, whereas beta-endosulfan was degraded by 4%, 60% and 67% after 30 days. ochrobacterum sp., arthrobacter sp., and burkholde ... | 2008 | 17646098 |
| chromate reduction by arthrobacter cr47 in biofilm packed bed reactors. | bacterial strain cr47 was isolated from a landfarming process soil sample. it was identified, by 16s rdna sequencing, as arthrobacter sp. the time course of the cr(vi) reduction was monitored in batch operated packed bed biofilm reactors (12 ml void volume) and in recirculating packed bed biofilm reactors (100 ml void volume) inoculated with bacterial strain cr47. the reduction was evaluated with, 30 mg l(-1) cr(vi) laboratory solutions prepared with k2cr2o7 and enriched with glucose-minimal med ... | 2008 | 18063473 |
| effect of immobilization on kinetic and thermodynamic characteristics of sulfide oxidase from arthrobacter species. | in order to determine the impact of immobilization on biocatalytic efficacy of sulfide oxidase, the kinetic and thermodynamic properties of native and deae-cellulose immobilized sulfide oxidase from arthrobacter species fr-3 were evaluated. immobilization increased the catalytic efficiency of sulfide oxidase by producing a lower michaelis-menten constant (km) and a higher rate of catalysis (vmax) at different temperatures. the first-order kinetic analysis of thermal denaturation demonstrated tha ... | 2008 | 18080911 |
| effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation. | spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ... | 2008 | 18083857 |
| degradation of pentachlorophenol by pure and mixed cultures in two different soils. | pentachlorophenol (pcp) is the second highest volume pesticide used in the united states. it is a mutagenic compound whose exposure poses significant health effects, one of the most desirable, environmentally friendly treatment methods is bioremediation. for soil-based contamination, the effectiveness of bioremediation will also be affected by the presence of an active indigenous population, sorption of the contaminant onto the soil, and environmental parameters. | 2007 | 17668821 |
| study of cadmium, zinc and lead biosorption by orange wastes using the subsequent addition method. | the biosorption of several metals (cd2+, zn2+ and pb2+) by orange wastes has been investigated in binary systems. multicomponent sorption isotherms were obtained using an original procedure, similar to that proposed by pagnanelli et al. [pagnanelli, f., petrangeli, m.p., toro, l., trifoni, m., veglio, f., 2001a. biosorption of metal ions on arthrobacter sp.: biomass characterization and biosorption modelling. environ. sci. technol. 34, 2773-2778] for monoelement systems, known as subsequent addi ... | 2008 | 18440805 |
| [restriction endonuclease asi256i recognizing the nucleotide sequence 5'-gatc-3']. | a strain producing a restriction endonuclease was isolated from soil samples and identified as the arthrobacter sp. strain ck256. the enzyme produced by this strain was termed asi2561. the isolation procedure for this enzyme was described, and the optimal conditions for its function were determined. it was shown that the restriction endonuclease asi256i is a true isoschizomer of mboi, it has a temperature optimum of 6 degrees c, and can be used in molecular-biological and genetic-engineering stu ... | 2008 | 18491594 |
| selection and identification of bacterial strains with methyl-tert-butyl ether, ethyl-tert-butyl ether, and tert-amyl methyl ether degrading capacities. | nine bacterial strains isolated from two hydrocarbon-contaminated soils were selected because of their capacity for growth in culture media amended with 200 mg/l of one of the following gasoline oxygenates: methyl-tert-butyl ether (mtbe), ethyl-tert-butyl ether (etbe), and tert-amyl methyl ether (tame). these strains were identified by amplification of their 16s rrna gene, using fdl and rd1 primers, and were tested for their capacity to grow and biotransform these oxygenates in both mineral and ... | 2008 | 18522454 |
| multi-compound electrophoretic assays for tyramine oxidase with a uv area detector imaging multiple windows on a looped capillary. | active pixel sensor uv area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (emma) assay for substrate specificity of tyramine oxidase (arthrobacter sp.). use of the uv area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. continuo ... | 2008 | 18589431 |
| [effect of a novel fibrinolytic enzyme fa-i on thrombosis and thrombolysis]. | to study the effect of fa-i, a novel fibrinolytic enzyme isolated from the metabolite of arthrobacter sp. dr-536, on thrombosis and thrombolysis in vivo. | 2009 | 19462909 |
| toxicity of homologous series of organic solvents for the gram-positive bacteria arthrobacter and nocardia sp. and the gram-negative bacteria acinetobacter and pseudomonas sp. | the toxicity of homologous series of organic solvents has been investigated for the gram-positive bacteria, arthrobacter sp. and nocardia sp., and the gram-negative bacteria, acinetobacter sp. and pseudomonas sp. the hydrophobicity of the solvent, expressed by its logp(octanol), proves to be a good measure for the toxicity of solvents in a two-phase system. the transition from toxic to nontoxic solvents occurs between logp(octanol) 3 and 5 and depends on the homologous series. no correlation has ... | 1993 | 18613108 |
| the screening, characterization, and use of omega-laurolactam hydrolase: a new enzymatic synthesis of 12-aminolauric acid. | several omega-laurolactam degrading microorganisms were isolated from soil samples. these strains were capable of growing in a medium containing omega-laurolactam as sole source of carbon and nitrogen. among them, five strains (t7, t31, u124, u224, and u238) were identified as cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, rhodococcus sp. u224, and sphingomonas sp. u238, respectively. the omega-laurolactam hydrolyzing enzyme from rhodococcus sp. u224 was purified to homogeneity, a ... | 2008 | 18685217 |
| genome sequence of the fish pathogen renibacterium salmoninarum suggests reductive evolution away from an environmental arthrobacter ancestor. | renibacterium salmoninarum is the causative agent of bacterial kidney disease and a significant threat to healthy and sustainable production of salmonid fish worldwide. this pathogen is difficult to culture in vitro, genetic manipulation is challenging, and current therapies and preventative strategies are only marginally effective in preventing disease. the complete genome of r. salmoninarum atcc 33209 was sequenced and shown to be a 3,155,250-bp circular chromosome that is predicted to contain ... | 2008 | 18723615 |
| enzymatic characterization of an amine oxidase from arthrobacter sp. used to measure phosphatidylethanolamine. | ethanolamine oxidase was screened with the aim of using it to establish a novel enzymatic phosphatidylethanolamine assay. ethanolamine oxidase activity was detected in the crude extract of arthrobacter sp., and the enzyme was purified more than 15-fold in three steps with a 54% yield. sds-page revealed the presence of only one band, which migrated, with an apparent molecular mass of 70 kda. biochemical characterization of the enzyme showed phenylethylamine to be the preferred substrate, with the ... | 2008 | 18838796 |
| plasmid as a measure of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid. | the purpose of this research was to pursuit the quantification of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid (2,4-d) by detecting and quantifying a prominent 2,4-d degradation encoding plasmid. batch reactor acclimation, de-acclimation, and re-acclimation tests were conducted during which periods the courses of 2,4-d dissipation and plasmid evolution were quantitatively measured. pure cultures of bacterial strains were detected to give rise to a plasmid approximately the s ... | 2009 | 18930390 |
| mineralization of p-nitrophenol by a new isolate arthrobacter sp. y1. | arthrobacter sp. y1, capable of metabolizing p-nitrophenol (pnp) as the sole carbon, nitrogen and energy source was isolated from activated sludge. the bacterium could tolerate concentrations of pnp up to 600 mg l(- 1), and degradation of pnp was achieved within 120 h of incubation. pnp and its metabolites were analyzed by high performance liquid chromatography (hplc). the metabolite formed indicated that the organism followed the 4-nitrocathechol (4-nc) pathway for metabolism of this compound. ... | 2008 | 18941993 |
| a novel aryl acylamidase from nocardia farcinica hydrolyses polyamide. | an alkali stable polyamidase was isolated from a new strain of nocardia farcinica. the enzyme consists of four subunits with a total molecular weight of 190 kda. the polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hydrolyzed different soluble amides to the corresponding acid. treatment of polyamide 6 with this amidase led to an increased hydrophilicity based on rising height and tensiometry mea ... | 2009 | 18942140 |
| metabolic cooperation of gordonia sp. strain mtcc 4818 and arthrobacter sp. strain wy in the utilization of butyl benzyl phthalate: effect of a novel co-culture in the degradation of a mixture of phthalates. | degradation of butyl benzyl phthalate (bbp) by a co-culture of gordonia sp. strain mtcc 4818 and arthrobacter sp. strain wy was investigated. in the degradation of bbp by the co-culture, the limitations of the individual species in metabolizing bbp were overcome, leading to the development of a consortium capable of complete utilization of this ester. in the degradation of bbp by the co-culture, the presence of multiple esterases was demonstrated in both species by activity staining of non-denat ... | 2008 | 18957587 |
| atrazine and terbuthylazine mineralization by an arthrobacter sp. isolated from a sugarcane-cultivated soil in kenya. | a tropical soil from a kenyan sugarcane-cultivated field showed a very high capability to mineralize (14)c-ring-labeled atrazine. in laboratory experiments this soil mineralized about 90% of the applied atrazine within 98 d. the atrazine-degrading microbial community was enriched in liquid cultures containing atrazine as the sole n source and 100 mgl(-1) glucose as additional c source. from the enrichment culture a bacterial strain was isolated and identified by comparative sequence analysis of ... | 2009 | 19674769 |
| isolation and characterization of mesotrione-degrading bacillus sp. from soil. | dissipation kinetics of mesotrione, a new triketone herbicide, sprayed on soil from limagne (puy-de-dôme, france) showed that the soil microflora were able to biotransform it. bacteria from this soil were cultured in mineral salt solution supplemented with mesotrione as sole source of carbon for the isolation of mesotrione-degrading bacteria. the bacterial community structure of the enrichment cultures was analyzed by temporal temperature gradient gel electrophoresis (ttge). the ttge fingerprint ... | 2009 | 19121884 |
| isolation and characterization of atrazine-degrading arthrobacter sp. ad26 and use of this strain in bioremediation of contaminated soil. | a bacterial strain (ad26) capable of utilizing atrazine as a sole nitrogen source for growth was isolated from an industrial wastewater sample by enrichment culture. the 16s rrna gene sequencing identified ad26 as an arthrobacter sp. pcr assays indicated that ad26 contained atrazine-degrading genes trzn and atzbc. the trzn gene of ad26 only differs from the trzn of arthrobacter aurescens tc1 by one base (a-->t at 907) and one amino acid (met-->leu at 303). the specific activity of trzn of ad26 i ... | 2008 | 19143347 |
| biodegradation of mixed phenolic compounds under high salt conditions and salinity fluctuations by arthrobacter sp. w1. | high salt concentration and salinity fluctuations in wastewater challenge the efficiency of microbial strains used for cleanup of pollutants. in this study, it was investigated that the new isolated arthrobacter sp. w1 degraded mixed phenolic compounds under complex salt conditions. the results showed that arthrobacter sp. w1 was able to utilize various phenolic compounds as carbon source under high salt conditions. it can degrade phenol and p-cresol mixture at 10% nacl, although rates of degrad ... | 2009 | 19156367 |
| preferential hydrolysis of aberrant intermediates by the type ii thioesterase in escherichia coli nonribosomal enterobactin synthesis: substrate specificities and mutagenic studies on the active-site residues. | the type ii thioesterase enth is a hotdog fold protein required for optimal nonribosomal biosynthesis of enterobactin in escherichia coli. its proposed proofreading activity in the biosynthesis is confirmed by its efficient restoration of enterobactin synthesis blocked in vitro by analogs of the cognate precursor 2,3-dihydroxybenzoate. steady-state kinetic studies show that enth recognizes the phosphopantetheine group and the pattern of hydroxylation in the aryl moiety of its thioester substrate ... | 2009 | 19193103 |
| arthrobacter phenanthrenivorans sp. nov., to accommodate the phenanthrene-degrading bacterium arthrobacter sp. strain sphe3. | a novel phenanthrene-degrading bacterium, designated strain sphe3(t), was isolated from a creosote-contaminated soil in greece. cells were non-motile, gram-positive, aerobic, and rod- to coccus-shaped. the strain was isolated on the basis of formation of a clear zone on agar plates sprayed with phenanthrene. optimal growth occurred at 30 degrees c. the g+c content of the dna was 65.7 mol%. the polar lipid pattern of strain sphe3(t) consisted of phosphatidylethanolamine, phosphatidylglycerol and ... | 2009 | 19196765 |
| purification and characterization of arsenite oxidase from arthrobacter sp. | the chemolithoautotroph, arthrobacter sp.15b oxidizes arsenite to arsenate using a membrane bound arsenite oxidase. the enzyme arsenite oxidase is purified to its homogeneity and identified using maldi-tof ms analysis. upon further characterization, it was observed that the enzyme is a heterodimer showing native molecular mass as approximately 100 kda and appeared as two subunits of approximately 85 kda lsu and 14 kda ssu on sds-page. the v(max) and k(m) values of the enzyme was found to be 2.45 ... | 2009 | 19214757 |
| global proteomic analysis of the chromate response in arthrobacter sp. strain fb24. | a global proteomic evaluation of the response of arthrobacter sp. strain fb24 to 5 and 20 mm cr(vi) was conducted using both two-dimensional gel electrophoresis (2-dge) and liquid chromatography coupled with tandem mass spectrometry (lc/lc-ms/ms). the changes in protein expression found with 2-dge indicate alterations in central metabolism and amino acid synthesis. proteome coverage increased from 22% with 2-dge to 71% with lc/lc-ms/ms. the proteins exhibiting the highest levels of expression un ... | 2009 | 19231868 |
| [isolation and identification of four dbp-degrading strains and molecular cloning of the degradation genes]. | four di-butyl-phthalate(dbp)-degrading bacterial strains, jdc-1, jdc-8, jdc-9 and jdc-12, were isolated from soil. the strains were gram positive. the 16s rrna sequence analysis revealed that the four strains had similarities of 99% with arthrobacter sp.. according to the morphologic, physiobiochemical characteristics and the analysis of their 16s rrna, all the four strains were identified as arthrobacter sp.. a 900 bp dna fragment was obtained from the four strains by pcr amplified and clone. w ... | 2009 | 19927831 |
| [isolation and degrading characters of chlorpyrifos degrading bacteria xz-3]. | a strain of bacterium xz-3 capable of highly degrading chlorpyrifos was screened from the soil sample collected from a pesticide plant after taming and enrichment. based on analysis of phenotype, physiological and biochemical characters and 16s rdna, xz-3 was identified as arthrobacter sp.. the effects of carbon source, ph, temperature and chlorpyrifos concentration on degradation were determined. the growth of bacteria in culture media were measured by visible absorption spectrophotometry under ... | 2007 | 18290445 |
| biofiltration of trimethylamine, dimethylamine, and methylamine by immobilized paracoccus sp. cp2 and arthrobacter sp. cp1. | a biofilter using granular activated carbon with immobilized paracoccus sp. cp2 was applied to the elimination of 10-250 ppm of trimethylamine (tma), dimethylamine (dma), and methylamine (ma). the results indicated that the system effectively treated ma (>93%), dma (>90%), and tma (>85%) under high loading conditions, and the maximum degradation rates were 1.4, 1.2, and 0.9g-nkg(-1) gac d(-1). among the three different amines treated, tma was the most difficult to degrade and resulted in ammonia ... | 2008 | 18331754 |
| improvement of catalytic properties of lipase from arthrobacter sp. by encapsulation in hydrophobic sol-gel materials. | in this work, lipase from arthrobacter sp. was immobilized by sol-gel encapsulation to improve its catalytic properties. various silanizing agents including vinyl-trimethoxy silane, octyl-trimethoxy silane, gamma-(methacryloxypropyl)-trimethoxy silane (maptms) and tetraethoxysilane (teos) were chosen as the precursors. among them, maptms was for the first time utilized to encapsulate lipases, and the prepared enzyme by copolymerization of maptms and teos exhibited the highest activity in both th ... | 2009 | 19428242 |
| heavy metal resistance in arthrobacter ramosus strain g2 isolated from mercuric salt-contaminated soil. | present study describes isolation of a multiple metal-resistant arthrobacter ramosus strain from mercuric salt-contaminated soil. the isolate was found to resist and bioaccumulate several metals, such as cadmium, cobalt, zinc, chromium and mercury. maximum tolerated concentrations for above metals were found to be 37, 525, 348, 1530 and 369 microm, respectively. the isolate could also reduce and detoxify redox-active metals like chromium and mercury, indicating that it has great potential in bio ... | 2010 | 20060643 |
| growth of arthrobacter sp. strain jbh1 on nitroglycerin as the sole source of carbon and nitrogen. | arthrobacter sp. strain jbh1 was isolated from nitroglycerin-contaminated soil by selective enrichment. detection of transient intermediates and simultaneous adaptation studies with potential intermediates indicated that the degradation pathway involves the conversion of nitroglycerin to glycerol via 1,2-dinitroglycerin and 1-mononitroglycerin, with concomitant release of nitrite. glycerol then serves as the source of carbon and energy. | 2010 | 20061454 |
| membrane bioreactor treatment of a simulated metalworking fluid wastewater containing ethylenediaminetetraacetic acid and dicyclohexylamine. | membrane bioreactors (mbrs) have been installed at automotive plants to treat metalworking fluid (mwf) wastewaters, which are known to contain toxic and/or recalcitrant organic compounds. a laboratory study was conducted to evaluate treatment of a simulated wastewater prepared from a semisynthetic mwf, which contains two such compounds, dicyclohexylamine (dcha) and ethylenediaminetetraacetic acid (edta). primary findings were as follows: during stable operating periods, almost all chemical oxyge ... | 2009 | 19445324 |
| [effect of elevation of 1-hydroxy-2-naphthoic acid concentration on the lag phase of arthrobacter sp. k3: kinetic analysis]. | | 2009 | 19449734 |
| microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes. | in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ... | 2009 | 19477544 |
| microbial responses to mustard gas dumped in the baltic sea. | microbiological studies were carried out on chemical weapon dump sites in the baltic sea. the effect of mustard gas hydrolysis products (mghps) on marine microbiota and the ability of microorganisms to degrade mghps were studied. many stations at the dump sites demonstrated reduced microbial diversity, and increased growth of species able to use mustard gas hydrolysis products as sole source of carbon. significant amounts of mghp-degrading bacteria were revealed in the near-bottom water. the mgh ... | 2009 | 19481794 |
| cg2091 encodes a polyphosphate/atp-dependent glucokinase of corynebacterium glutamicum. | the corynebacterium glutamicum gene cg2091 is encoding a polyphosphate (polyp)/atp-dependent glucokinase (ppgk). previous work demonstrated the association of ppgk to polyp granules. the deduced amino acid sequence of ppgk shows 45% sequence identity to polyp/atp glucomannokinase of arthrobacter sp. strain km and 50% sequence identity to polyp glucokinase of mycobacterium tuberculosis h37rv. ppgk from c. glutamicum was purified from recombinant escherichia coli. polyp was highly preferred over a ... | 2010 | 20379711 |
| batch and continuous biosorption of cu(2+) by immobilized biomass of arthrobacter sp. | the ability of free and polysulphone immobilized biomass of arthrobacter sp. to remove cu(2+) ions from aqueous solution was studied in batch and continuous systems. the langmuir and freundlich isotherm models were applied to the data. the langmuir isotherm model was found to fit the sorption data indicating that sorption was monolayer and uptake capacity (q(o)) was 175.87 and 158.7mg/g for free and immobilized biomass respectively at ph 5.0 and 30 degrees c temperature, which was also confirmed ... | 2009 | 19487070 |
| optimization of physical parameters for lipase production from arthrobacter sp. bgcc#490. | the physical parameters for the production of thermostable, alkaline lipase from arthrobacter sp. bgcc# 490 were optimized using response surface methodology (rsm), employing face centered central composite design (fcccd). the design was employed by selecting ph, temperature and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by rsm. a second-order quadratic model and response surface method showed that the optimum conditions for lipa ... | 2009 | 19517996 |
| isolation and characterization of arthrobacter sp. hy2 capable of degrading a high concentration of p-nitrophenol. | a soil bacterium strain, capable of using p-nitrophenol (pnp) as its sole source of carbon and energy, was isolated by enrichment on minimal salt medium (msm). on the basis of a phylogenetic analysis of 16s rrna gene sequences the bacterium is a species of arthrobacter, closely related to arthrobacter ureafaciens dsm 20126. this strain has an unusually high substrate tolerance for pnp degradation in msm. greatest degradation of pnp was observed at 30 degrees c and under slightly alkaline ph (ph ... | 2009 | 19540107 |
| a new cold-adapted beta-d-galactosidase from the antarctic arthrobacter sp. 32c - gene cloning, overexpression, purification and properties. | the development of a new cold-active beta-d-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. | 2009 | 19631003 |
| high-density polyethylene (hdpe)-degrading potential bacteria from marine ecosystem of gulf of mannar, india. | assessment of high-density polyethylene (hdpe)-degrading bacteria isolated from plastic waste dumpsites of gulf of mannar. | 2010 | 20586938 |
| monitoring enzyme catalysis in the multimeric state: direct observation of arthrobacter 4-hydroxybenzoyl-coenzyme a thioesterase catalytic complexes using time-resolved electrospray ionization mass spectrometry. | the ability to examine real-time reaction kinetics for multimeric enzymes in their native state may offer unique insights into understanding the catalytic mechanism and its interplay with three-dimensional structure. in this study, we have used a time-resolved electrospray mass spectrometry approach to probe the kinetic mechanism of 4-hydroxybenzoyl-coenzyme a (4-hba-coa) thioesterase from arthrobacter sp. strain su in the millisecond time domain. intact tetrameric complexes of 4-hba-coa thioest ... | 2009 | 19635449 |
| air-cathode microbial fuel cell array: a device for identifying and characterizing electrochemically active microbes. | microbial fuel cells (mfcs) have generated excitement in environmental and bioenergy communities due to their potential for coupling wastewater treatment with energy generation and powering diverse devices. the pursuit of strategies such as improving microbial cultivation practices and optimizing mfc devices has increased power generating capacities of mfcs. however, surprisingly few microbial species with electrochemical activity in mfcs have been identified because current devices do not suppo ... | 2011 | 20655725 |
| effects of bacterial treatment at early stages of atlantic cod (gadus morhua l.) on larval survival and development. | to assess the effects of bacterial treatment at the earliest stages of cod rearing on the microbial load, larval development and performance, testing three bacterial strains (carnobacterium divergens v41, arthrobacter sp. and enterococcus sp.) in vivo that were previously shown to have inhibitory potential towards fish pathogens in vitro. | 2010 | 19664064 |
| genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition. | in this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for plant growth promoting (pgp) traits at higher salt (nacl) concentrations (2, 4, 6, and 8%). the results revealed that 24 rhizobacterial isolates were tolerant at 8% nacl. although all the 24 salt tolerable isolates produced indole-3-acetic acid (iaa), while 10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. however, only three isol ... | 2009 | 19701667 |
| fluorinated phenylcyclopropylamines. part 6: effects of electron withdrawing or donating aryl substituents on the inhibition of tyramine oxidase from arthrobacter sp. by diastereomeric 2-aryl-2-fluoro-cyclopropylamines. | diastereomeric arylcyclopropylamines substituted with fluorine in the 2-position and with electron donating or electron withdrawing groups at the aromatic ring were evaluated as inhibitors of microbial tyramine oxidase. the trans-isomers were consistently more potent inhibitors of the enzyme than the cis-isomers. electron donating substituents increased the potency of tyramine oxidase inhibition, while electron withdrawing substituents decreased the activity. the results obtained are discussed i ... | 2008 | 19727324 |
| high-level chromate resistance in arthrobacter sp. strain fb24 requires previously uncharacterized accessory genes. | the genome of arthrobacter sp. strain fb24 contains a chromate resistance determinant (crd), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. the crd includes chra, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino and carboxy termini of chrb, a putative regulatory protein. there are also three novel genes that have not been previously associated with chromate resistance in other bacteria; they encode a ... | 2009 | 19758450 |
| a new beta-galactosidase with a low temperature optimum isolated from the antarctic arthrobacter sp. 20b: gene cloning, purification and characterization. | a psychrotrophic bacterium producing a cold-adapted beta-galactosidase upon growth at low temperatures was classified as arthrobacter sp. 20b. a genomic dna library of strain 20b introduced into escherichia coli top10f' and screening on x-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside)-containing agar plates led to the isolation of beta-galactosidase gene. the beta-galactosidase gene (bgas) encoding a protein of 1,053 amino acids, with a calculated molecular mass of 113,695 kda. analys ... | 2009 | 19771412 |
| soil bacterial communities in constructed wetlands treated with swine wastewater using pcr-dgge technique. | marsh-pond-marsh (mpm) constructed wetlands were designed for the treatment of swine wastewater. the goal of this study was to characterize bacterial communities in these wetlands and determine the nutrient removal from influent to effluent. surface soil samples were collected and analyzed by culture-dependent and culture-independent techniques. the results showed that the bacterial colony forming units (cfu) and the average concentrations of total nitrogen, nh(4)(+), total phosphorous (tp) and ... | 2010 | 19822421 |
| analysis of two gene clusters involved in the degradation of 4-fluorophenol by arthrobacter sp. strain if1. | arthrobacter sp. strain if1 is able to grow on 4-fluorophenol (4-fp) as a sole source of carbon and energy. to clone the 4-fp degradation genes, dna libraries were constructed and screened with a probe obtained by pcr using primers designed on the basis of conserved regions of aromatic two-component monooxygenases. sequencing of positive clones yielded two gene clusters, each harboring a gene encoding a monooxygenase with high sequence similarity to the oxygenase component of 4-nitrophenol and 4 ... | 2009 | 19837837 |
| x-ray crystallographic analysis of the 6-aminohexanoate cyclic dimer hydrolase: catalytic mechanism and evolution of an enzyme responsible for nylon-6 byproduct degradation. | we performed x-ray crystallographic analyses of the 6-aminohexanoate cyclic dimer (acd) hydrolase (nyla) from arthrobacter sp., an enzyme responsible for the degradation of the nylon-6 industry byproduct. the fold adopted by the 472-amino acid polypeptide generated a compact mixed alpha/beta fold, typically found in the amidase signature superfamily; this fold was especially similar to the fold of glutamyl-trna(gln) amidotransferase subunit a (z score, 49.4) and malonamidase e2 (z score, 44.8). ... | 2010 | 19889645 |
| industrial production of difructose anhydride iii (dfa iii) from crude inulin extracted from chicory roots using arthrobacter sp. h65-7 fructosyltransferase. | a practical, economical, and industrial process for the enzymatic production of difructose anhydride iii (dfa iii) was investigated for crude inulin prepared from chicory roots using arthrobacter sp. h65-7 fructosyltransferase. a comparable level of dfa iii production to that from commercial inulin was obtained using crude inulin, suggesting the feasibility of this production process. | 2009 | 19269589 |
| high rate of n2 fixation by east siberian cryophilic soil bacteria as determined by measuring acetylene reduction in nitrogen-poor medium solidified with gellan gum. | for evaluating n(2) fixation of diazotrophic bacteria, nitrogen-poor liquid media supplemented with at least 0.5% sugar and 0.2% agar are widely used for acetylene reduction assays. in such a soft gel medium, however, many n(2)-fixing soil bacteria generally show only trace acetylene reduction activity. here, we report that use of a n(2) fixation medium solidified with gellan gum instead of agar promoted growth of some gellan-preferring soil bacteria. in a soft gel medium solidified with 0.3% ge ... | 2009 | 19286791 |
| effect of immobilization of a bacterial consortium on diuron dissipation and community dynamics. | this work intended to study the relationship between diuron herbicide dissipation and the population dynamics of co-cultivated delftia acidovorans wdl34 (wdl34) and arthrobacter sp. n4 (n4) for different cell formulations: free cells or immobilization in ca-alginate beads of one or both strains. gfp-tagged wdl34 and n4 gram staining allowed analyzing the cell growth and distribution of each strain in both beads and culture medium in the course of the time. compared to the free cell co-culture of ... | 2009 | 19386487 |
| high yield synthesis of 12-aminolauric acid by "enzymatic transcrystallization" of omega-laurolactam using omega-laurolactam hydrolase from acidovorax sp. t31. | the genes encoding omega-laurolactam hydrolases from cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, and sphingomonas sp. u238 were cloned and sequenced. nucleotide and amino acid sequence analysis of the four genes indicated that the primary structures of these omega-laurolactam hydrolases are significantly similar to the 6-aminohexanoate-cyclic-dimer hydrolase (ec 3.5.2.12). these genes were expressed in escherichia coli, and the omega-laurolactam hydrolysing activity of the reco ... | 2009 | 19420719 |
| [monosaccharide composition of exopolymer complex of bacteria-destructors of protective coatings]. | monosaccharide composition of exopolymer complex (epc) ofbacteria-destructors of protective coatings pseudomonas sp. t/, pseudomonas sp. 109, arthrobacter sp. 102 has been studied. it is established that monosaccharide composition of exopolymer complex of bacteria is different and depends on the bacteria growth model. it is mainly represented by penthoses and hexoses. glucose, galactose, arabinose, which are determined in epc of biofilm and plankton of all studied bacteria, are dominating monosa ... | 2009 | 19938612 |
| complete degradation of di-n-octyl phthalate by biochemical cooperation between gordonia sp. strain jdc-2 and arthrobacter sp. strain jdc-32 isolated from activated sludge. | two bacterial strains were isolated from activated sludge using mixtures of phthalic acid esters (paes) as the sole source of carbon and energy. one of the isolates was identified as gordonia sp. strain jdc-2 and the other as arthrobacter sp. strain jdc-32, mainly through 16s rrna gene sequence analysis. gordonia sp. strain jdc-2 rapidly degraded di-n-octyl phthalate (dop) into phthalic acid (pa), which accumulated in the culture medium. arthrobacter sp. strain jdc-32 degraded pa but not dop. th ... | 2010 | 19959291 |
| isolation of an isocarbophos-degrading strain of arthrobacter sp. scl-2 and identification of the degradation pathway. | isocarbophos is a widely used organophosphorus insecticide that has caused environmental pollution in many areas. however, degradation of isocarbophos by pure cultures has not been extensively studied, and the degradation pathway has not been determined. in this paper, a highly effective isocarbophos-degrading strain, scl-2, was isolated from isocarbophos-polluted soil. strain scl-2 was preliminarily identified as arthrobacter sp. based on its morphological, physiological, and biochemical proper ... | 2009 | 19996699 |
| influence of internal biofilm growth on residual permeability loss in aerobic granular membrane bioreactors. | membrane fouling results in flux decline or transmembrane pressure drop increase during membrane bioreactor (mbr) operation. physical and chemical cleanings are essential to keep an mbr operating at an appropriate membrane flux. considerable residual membrane permeability loss that cannot be removed by conventional cleaning requires membrane replacement. this study demonstrates that an internal biofilm can develop inside a hollow-fiber membrane and can probably account for up to 58.9 and 81.3% o ... | 2010 | 20102183 |
| the biomass yielding process of xenobiotic degradation. | yields of activated sludge and an arthrobacter sp. biomass on organic xenobiotic 2,4-dichlorophenoxyacetic acid (2,4-d) and on the intermediates of selected 2,4-d metabolism pathways were measured. activated sludge yield on 2,4-d was lower by approximately 24-45% compared to the combined yields produced separately by the lower intermediates. for activated sludge, cell synthesis only consumed 33% of the electrons generated from 2,4-d oxidation, while the other 67% were used for energy. the high e ... | 2010 | 20153175 |
| strains of internal biofilm in aerobic granular membrane bioreactors. | this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ... | 2010 | 20306189 |
| arthrobacter sp. strain ku001 isolated from a thai soil degrades atrazine in the presence of inorganic nitrogen sources. | an atrazine degrading bacterium, strain ku001, was obtained from a sugarcane field at the cane and sugar research and development center at the kasetsart university, kamphaeng saen campus, thailand. strain ku001 had a rod-to-coccus morphological cycle during growth. biolog carbon source analysis indicated that the isolated bacterium was arthrobacter histidinolovorans. sequence analysis of pcr product indicated the 16s rrna gene in strain ku001 was 99% identical to the same region in arthrobacter ... | 2010 | 20372034 |
| comparative study of properties of immobilized lipase onto glutaraldehyde-activated amino-silica gel via different methods. | the enzyme-aggregate coating method was performed to immobilize arthrobacter sp. lipase in order to achieve better catalytic properties comparable to the conventional covalent attachment and covalent attachment plus cross-linking. the glutaraldehyde-activated amino-silica gel which was synthesized by sol-gel technique was used as the support, and the catalytic characteristics of the lipase preparations were tested in the asymmetric acylation of 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-o ... | 2010 | 20399626 |
| homology modeling and docking study of xanthine oxidase of arthrobacter sp. xl26. | hyperuricemia is a condition of defective purine metabolism characterized with elevated serum uric acid (ua) level that further leads to gout and gouty nephrolithiasis disorders. gout is a world wide distributed rheumatic disease comprises 1% of the total population and still is in increasing state. one of the factors contributing to overproduction of ua is the hydroxylation of xanthine catalyzed by xanthine oxidase (xo). in the present study, 3d modeling of arthrobacter sp. xl26 (xodb) protein ... | 2010 | 20403378 |
| weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution. | several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ... | 2010 | 20525872 |
| influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments. | because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ... | 2010 | 20553043 |
| study on the removal of indoor vocs using biotechnology. | with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ... | 2010 | 20598440 |
| isolation and characterization of an arthrobacter sp. strain hb-5 that transforms atrazine. | a bacterial strain (hb-5) capable of utilizing atrazine as sole carbon and nitrogen source for growth was isolated from an industrial wastewater sample by enrichment culture. the isolate was identified as arthrobacter sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. the strain exhibited faster atrazine degradation rates in atrazine-containing mineral media than the well-characterized atrazine-degrading bacteria pseudomonas sp. adp. ... | 2011 | 20686824 |