| nitrate reductase and dehydrogenase activities of cowpea nodules as affected by ammonium sulphate and urea fertilizers. | | 1978 | 726712 |
| the correlation between the efficiency of rhizobia and nitrate reductase and dehydrogenase activities of cowpea nodules. | cowpea seeds variety fettriat were planted in nile silt soil and inoculated with 5 strains of cowpea rhizobia. after 50 and 80 days, the plants were uprooted, analysed for dry weight, total nitrogen, fresh weight of nodules, nitrate reductase activity in the leaves, and nitrate reductase and dehydrogenase activities in the nodule homogenate in the presence or absence of succinate, citrate, and ethyl alcohol. the data were analysed to establish the correlation coefficients between total nitrogen ... | 1978 | 726713 |
| studies on bacteroid size and nucleic acid content of alfalfa bacteroids fractionated by velocity sedimentation. | a velocity sedimentation procedure was described to fractionate bacteroids of alfalfa nodules into four subpopulations. bacteroids in these subpopulations were different in size and nucleic acid content as determined by microscopy and flow-microfluorometry (fmf). the slowest-sedimenting bacteroids (fraction i) were small and resembled free-living rhizobium meliloti both in size and nucleic acid content. the fastest-sedimenting bacteroids (fraction iv) were 2 to 3 times longer and contained 3 to ... | 1978 | 728854 |
| plasmid-dependent attachment of agrobacterium tumefaciens to plant tissue culture cells. | kinetic, microscopic, and biochemical studies show that virulent ti (tumor inducing)-plasmid-containing strains of agrobacterium attach to normal tobacco and carrot tissue culture cells. kinetic studies showed that virulent strains of a. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. five ti-plasmid-containing virulent agrobacterium strains showed greater attachment to tobacco cells than did ... | 1978 | 730370 |
| varieties of mutualistic interaction in population models. | | 1978 | 732344 |
| nodule infection by bean yellow mosaic virus in phaseolus vulgaris. | infection of root nodules of beans, phaseolus vulgaris l., by bean yellow mosaic virus (bymv) and the effect of the disease on the specific activity of the nodule are reported. infectivity and serological microprecipitin assays with two sources of bymv antiserum demonstrated that nodules from bean plants whose leaves had been inoculated with bymv contain bymv antigen. the disease reduced the fresh weights of tops, roots, and root nodules and induced premature nodule decay and/or nodule drop. the ... | 1978 | 736539 |
| [production of mutants of nodule bacteria which have lost their ability to reduce acetylene]. | | 1978 | 745564 |
| round table discussion: long-range research planning in carbon assimilation. | | 1978 | 747609 |
| rhizobium strain identification in arachis hypogaea nodules by enzyme-linked immunosorbent assay (elisa). | the technique of enzyme-linked immunosorbent assay (elisa) was used for serological identification of peanut rhizobium strains both in cell suspension of pure culture and in single root nodules of groundnut (arachis hypogaea) plants. antisera of three peanut rhizobium strains were tested against eight different rhizobium isolates. three serogroups identified by agglutination and immunodiffusion tests were confirmed by elisa. in this experiment elisa was more sensitive by four to six orders of ma ... | 1978 | 747814 |
| mutagenesis by insertion of the drug resistance transposon tn7 applied to the ti plasmid of agrobacterium tumefaciens. | | 1978 | 748948 |
| fingerprints of agrobacterium ti plasmids. | | 1978 | 748949 |
| restriction endonuclease mapping of a plasmid that confers oncogenicity upon agrobacterium tumefaciens strain b6-806. | | 1978 | 748950 |
| production of adventitious root primordia on hypocotyls of castor bean seedling, infected with agrobacterium tumefaciens. | the development of sterile secondary tumours on hypocotyls of castor bean seedlings, inoculated with any of the ten isolates of agrobacterium tumefaciens tested, were observed below the site of the primary tumours. histopathological studies performed in the present work indicate that the observed secondary tumours were adventitious root primordia and not the ordinary type of secondary tumours. according to the available literature such findings are reported here for the first time. all the teste ... | 1978 | 749411 |
| induction of polygalacturonase production in legume roots as a consequence of extrachromosomal dna carried by rhizobium meliloti. | the ability of rhizobium meliloti to induce polygalacturonase production in legume roots decreased during culture under laboratory conditions but was inducible with mitomycin c. this character was irreversibly lost after treatment with acridine orange. extrachromosomal dna of molecular weight 5.9 x 10(6) daltons was detectable in neutral sucrose gradient but was absent from cells 'cured' with acridine orange. therefore, the ability to induce the enzyme production may be controlled by a plasmid. | 1978 | 750886 |
| ability of free-living nodule bacteria to fix atmospheric nitrogen. | literature material is presented on the ability of free-living nodule bacteria for asymbiotic nitrogen fixation, detected for the first time in 1975. necessary components of the nutrient medium in the use of which the nitrogen-fixing ability of rhizobia in pure cultures is manifested, proved to be sugars and intermediates of the citric acid cycle, as well as small quantities of bound nitrogen. the experimental data available in the literature are evidence of the presence of a complete assortment ... | 1978 | 751688 |
| quantitative cytochemistry of glycogen in cells of pea and lupine nodule bacteria. | using a fluorescent variation of the method of pas reaction with auramine oo-so2, the content of pas-positive substance, identified on the basis of supplementary treatments of the preparations with alpha-amylase and a hot mixture of chloroform with methanol and glycogen, was determined cytofluorimetrically in cells of effective and ineffective strains of lupine and pea nodule bacteria in pure culture and under conditions of symbiosis. the largest level of glycogen was detected in bacteroid forms ... | 1978 | 751689 |
| electron microscopic study of the rhizosphere in situ. | | 1978 | 751698 |
| inhibitory effects of the herbicide trifluralin on the establishment of the clover root nodule symbiosis. | trifluralin, a widely used herbicide (rahman, 1977), may act via interaction with plant microtubule protein (hess and bayer, 1974, 1977). in trifluralin treated seedlings of trifolium repens, root tips increased in diameter and decreased in length, cell elongation was decreased, cell wall deposition was abnormal, root hairs were deformed, and a marked reduction was seen in the number of infection threads induced by the bacterial symbionts. our observations support the hypothesis that trifluralin ... | 1978 | 751777 |
| adaptability of rhizobium meliloti in callus tissues of alfalfa roots. | the establishment of associative interrelations between nodule bacteria and cells of a callus culture of alfalfa roots, evaluated according to the criterion of nitrogenase activity, was accompanied by considerable inhibition of the plant cells in the period of maximum infection as well as by the dispersal of actively multiplying nodule bacteria on the surface of the callus cells in the first 7 days after infection. the dynamics of the localization of nodule bacteria on the surface of callus cell ... | 1978 | 754813 |
| action of metabolites of isolated plant tissues on the nitrogenase activity of rhizobium vigna and rhizobium meliloti. | the dependence of the nitrogenase activity of rhizobium meliloti on the strain peculiarities of the cultures, the composition of the media used, and the metabolites of legume tissue cultures was demonstrated by the acetylene method. the nitrogenase activity is significantly higher in r. vigna than in r. meliloti, under the same experimental conditions. enrichment of the murashige-skoog medium with arabinose (25 mm), succinate (25 mm), glutamine (2 mm nitrogen), and yeast extract (0.1%) substanti ... | 1978 | 754814 |
| transfer, maintenance, and expression of bacterial ti-plasmid dna in plant cells transformed with a. tumefaciens. | the mechanism of induction of the plant cancer crown gall by agrobacterium tumefaciens has been briefly described. the salient points are as follows. 1. large plasmids of molecular weight (100 to 150) s 10(6), called ti-plasmids, are essential to the transformation process. 2. ti-plasmids carry a dna segment that can be transferred to, and maintained and expressed in, transformed plant cells. 3. this dna segment has been identified both by direct hybridization experiments between ti dna fragment ... | 1977 | 754869 |
| quality and rate of extracellular polysaccharides produced by rhizobium meliloti and their inducing effect on polygalacturonase production in legume roots as derived from the presence of extrachromosomal dna. | the ability of extracellular polysaccharides of different strains of rhizobium meliloti to induce the production of polygalacturonase by roots of medicago sativa seedlings has been studied. some strains showed a high inducing activity while those derived from them, after treatment with acridine orange and in which extrachromosomal dna was absent, did not show this character. a comparative study of polysaccharide production and preliminary studies on the chemical composition of the active fractio ... | 1978 | 755154 |
| effect of sublethal heat injury on tumour induction and rna synthesis in agrobacterium tumefaciens. | when cells of agrobacterium tumefaciens are subjected to sublethal heat injury at 45 degrees c for 20 min, less than 5% of the viable population retain their ability to initiate tumour formation on kalanchöe daigremontiana. if the cells are then incubated in phosphate buffer at 27 degrees c for 2 h, tumour initiation returns to control levels. inhibitors of dna and protein synthesis had little effect on the recovery of tumour initiation after heat injury. rifamycin, a specific inhibitor of rna s ... | 1978 | 756950 |
| low-intensity microwave radiation and the virulence of agrobacterium tumefaciens strain b6. | when virulent cells of agrobacterium tumefaciens strain b6 were exposed to low-level microwave radiation at a frequency of 10,000 mhz and an intensity of 0.58 mw/cm2 for 30 to 120 min, a 30 to 60% decrease in their ability to produce tumors on potato and turnip disks was observed. this microwave exposure did not affect the viability of these bacteria or their ability to attach to a tumor-binding site nor did it induce thermal shock. this loss of virulence was reversible within 12 h. | 1979 | 760631 |
| hydrogen-dependent nitrogenase activity and atp formation in rhizobium japonicum bacteroids. | rhizobium japonicum 122 des bacteroids from soybean nodules possess an active h(2)-oxidizing system that recycles all of the h(2) lost through nitrogenase-dependent h(2) evolution. the addition of 72 mum h(2) to suspensions of bacteroids increased o(2) uptake 300% and the rate of c(2)h(2) reduction 300 to 500%. the optimal partial pressure of o(2) was increased, and the partial pressure of o(2) range for c(2)h(2) reduction was extended by adding h(2). a supply of succinate to bacteroids resulted ... | 1979 | 762010 |
| phosphoglucose isomerase mutant of rhizobium meliloti. | a mutant strain of complex phenotype was selected in rhizobium meliloti after nitrosoguanidine mutagenesis. it failed to grow on mannitol, sorbitol, fructose, mannose, ribose, arabitol, or xylose, but grew on glucose, maltose, gluconate, l-arabinose, and many other carbohydrates. assay showed the enzyme lesion to be in phosphoglucose isomerase (pgi), and revertants, which were of normal growth phenotype, contained the enzyme again. nonpermissive substrates such as fructose and xylose prevented g ... | 1979 | 762017 |
| alpha-ketoglutarate dehydrogenase mutant of rhizobium meliloti. | a mutant of rhizobium meliloti selected as unable to grow on l-arabinose also failed to grow on acetate or pyruvate. it grew, but slower than the parental strain, on many other carbon sources. assay showed it to lack alpha-ketoglutarate dehydrogenase (kgd) activity, and revertants of normal growth phenotype contained the activity again. other enzymes of the tricarboxylic acid cycle and of the glyoxylate cycle were present in both mutant and parent strains. enzymes of pyruvate metabolism were als ... | 1979 | 762018 |
| construction of a p plasmid carrying nitrogen fixation genes from klebsiella pneumoniae. | | 1976 | 768778 |
| the molybdenum--iron protein of klebsiella pneumoniae nitrogenase. evidence for non-identical subunits from peptide 'mapping'. | the molybdenum- and iron-containing protein components of nitrogenase purified from klebsiella pneumoniae, azotobacter vinelandii, azotobacter chroococcum and rhizobium japonicum bacteroids all gave either one or two protein-staining bands after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, depending on the commercial brand of sodium dodecyl sulphate used. the single band obtained with k. pneumoniae mo-fe protein when some commercial brands of sodium dodecyl sulphate were used in t ... | 1976 | 779772 |
| conjugation in agrobacterium tumefaciens in the absence of plant tissue. | a general, reliable conjugation system for agrobacterium tumefaciens in the absence of plant tissue is described in which a. tumefaciens can serve either as the donor or recipient of plasmid deoxyribonucleic acid with reasonable efficiency. plasmid rp4 was transferred from escherichia coli to a. tumefaciens and from strain of a. tumefaciens. both rp4 and the a. tumefaciens virulence-associated plasmids were detected by alkaline sucrose gradients in a. tumefaciens strains a6 and c58 after mating ... | 1976 | 783141 |
| properties of plasmids constructed by the in vitro insertion of dna from rhizobium leguminosarum or proteus mirabilis into rp4. | plasmids have been constructed by insertion of dna from rhizobium leguminosarum or proteus mirabilis into rp4 (an r factor of group p). such recombinant plasmids retain the wide host range of the parental plasmid, being as efficiently transmissible as the unmodified rp4 and are stably maintained in rapidly growing cultures. the recombinant plasmids, even though each contained a dna sequence absolutely identical with that of the host strain, are no more efficient at mobilizing the transfer of chr ... | 1976 | 787766 |
| inhibitory effect of streptomyces antibioticus and other microorganisms on rhizobium. | | 1976 | 789197 |
| [selection methods of nodule bacteria]. | the aim of selecting nodule bacteria is to obtain stable and genetically pure strains with elevated effectiveness in the conditions of symbiosis with bean plants, sufficient viability and competing ability, which would survive in soil for a long time. the following methods have been suggested to solve this task: selection by plants using mutagenic factors; genetic markers; production of genetically homogeneous material; determination of the degree of persistance effectiveness in mutants. | 1976 | 794638 |
| [molecular nitrogen fixation by pure cultures of nodular bacteria (a literature review)]. | | 1976 | 800021 |
| immunological evidence for the capability of free-living rhizobium japonicum to synthesize a portion of a nitrogenase component. | immunodiffusion tests conducted under aerobic conditions demonstrated that cross-reactive material to antiserum prepared against the mo-fe protein component of nitrogenase from soybean nodule bacteroids was detectable in extracts of free-living rhizobium japonicum cells cultured in a standard medium under: aerobic conditions; aerobic conditions with nitrate; aerobic conditions with ammonia; anaerobic conditions with nitrate; and anaerobic conditions with nitrate and ammonia. the most intense pre ... | 1975 | 803382 |
| [regulation of nitrogenase biosynthesis in microorganisms]. | | 1975 | 803994 |
| protozoa and the decline of rhizobium populations added to soil. | a fall in rhizobium abundance occurred in nonsterile soil inoculated with large numbers of the root-nodule bacteria, but many of the rhizobia still survived. no such decline was evident in sterile soil. protozoa feeding on these bacteria were isolated from soil and other environments. as the abundance of rhizobium meliloti and a cowpea rhizobium strain in soil decreased, the protozoan density increased. the inability of the predators to eliminate their prey from soil was not the result of the pr ... | 1975 | 807307 |
| [cytokinins in microorganisms]. | | 1975 | 808806 |
| antigenic differences between infective and noninfective strains of rhizobium trifolii. | immunodiffusion and immunoelectrophoresis techniques have revealed the presence of soluble antigens in sonicated preparations of four infective strains of rhizobium trifolii which were absent in similar preparations of related noninfective mutants derived from the infective strains. the soluble antigens unique to the infective strains were cross-reactive with one another. | 1975 | 809004 |
| on the question of the integration of exogenous bacterial dna into plant dna. | extensive studies with pea, tomato, and barley failed to confirm the evidence presented by previous investigators for integration or replication of exogenously applied bacterial dna in these plants. labeled dna of buoyant density in cscl intermediate between that of high density donor bacterial dna and of plant dna was never observed with axenic plants. intermediate peaks, similar to those used as evidence for recombination by earlier investigators, were observed only when the plants were contam ... | 1975 | 809769 |
| [an current question in the molecular genetics of bacteria]. | the author considers the possibilities and limits of extrapolation of the data by the genetics of one species of bacteria to the other. it is emphasized that even in the related bacterial species a similar localization on chromosomes was inherent only to some of the unitypical genes, by in this case as well not all the genes were grouped in the same way, and differed by their delicate structure. an idea on the significant role of genetic metabolism in the microbial evolution is being developed; ... | 1975 | 813460 |
| studies on phage 1p receptors in rhizobium trifolii and rhizobium leguminosarum. | the rate of phage 1p attachment to rhizobium cell walls was increased in the presence of ethylenediaminetetraacetic acid (edta). on the other hand the rate of adsorption of phage 1p to the triton -- insoluble cell walls was diminished. the subsequent treatment of cell walls with 2% triton x-100 and 5mm edta caused a more substantial decline of the phage inactivating capacity. lipopolysaccharides (lps) isolated from the sensitive strains, contrary to those from phage-resistant mutants, inactivate ... | 1975 | 813494 |
| degradation of 4-aminopyridine in soil. | | 1976 | 818143 |
| ribonucleotide reductase in blue-green algae: dependence on adenosylcobalamin. | ten species of freshwater blue-green algae exhibit an adenosylcobalamin-dependent ribonucleotide reductase, thuse explaining the requirement for cobalt by these organisms. the evidence suggests a phylogenetic affinity between the cyanophytes and bacteria, such as clostridium and rhizobium, and the euglenoid flagellates, which also use the cofactor-dependent reductase. in contrast, the ribonucleotide reductase reaction in the few green algae surveyed shows no dependence on cobalamins. | 1976 | 818711 |
| discrimination of rhizobium japonicum, rhizobium lupini, rhizobium trifolii, rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate. | rhizobium strains (one each of rh. japonicum, rh. lupini, rh. leguminosarum) take up 2-ketoglutaric acid in general much faster and from lower concentrations in the medium than strains of escherichia coli, bacillus subtilis and chromobacterium violaceum. a strain of enterobacter aerogenes, however, is more similar to some rhizobium strains. the same strains of rhizobium take up also phosphate much faster and from lower concentrations than the other bacteria tested. 4 strains of rh. lupini proved ... | 1976 | 818969 |
| improved method for preparing anaerobic bacteroid suspensions of rhizobium leguminosarum for the acetylene reduction assay. | a method using ethylenediaminetetraacetate (edta) and toluene-treated pea bacteroid suspensions for the acetylene reduction assay is described. the high level of acetylene reduction by these bacteroids is comparable to that of intact plants. reproducibility of the edta-toluene treatment is, on the average, within 5%. preliminary experiments with soybeans indicate that the edta-toluene method might be applicable to other legumes as well. | 1976 | 820257 |
| [conditions for bacterial transfection]. | | 1976 | 822657 |
| [enzymatic reduction of legoglobin]. | during incubation in the buffer bacteroids rizobium lupini liberate an enzymic system (ferri-lb-reductase) capable of reducing ferri-lb and ferri-mb the hemoproteids reduction required the presence of cofactors (nadph or nadh). the ferri-lb-reductase activity was also found in the soluble fraction of nodules. it is assumed that bacteroids ferri-lb-reductase, maintains lb in a physiologically active reduced state into vegetable cells of the nodules "in vivo" as well. | 1976 | 826285 |
| chromosomal recombination and mapping in rhizobium leguminosarum. | | 1976 | 826827 |
| octopine and nopaline metabolism in agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes. | crown gall tumors produced octopine or nopaline or neither compound, depending on the bacterial strain that incited the tumor. the genes specifying production of octopine or nopaline by the tumor were transferred to recipient bacterial strains when the large plasmid associated with virulence was transferred by either conjugation or deoxyribonucleic acid-mediated transformation. our results, which confirm the work of others (bomhoff et al., 1976; goldman et al., 1968; petit et al., 1970), indicat ... | 1977 | 830636 |
| plasmids in avirulent strains of agrobacterium. | twelve strains of agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, kalanchoe, and carrot. eleven strains contained plasmids of molecular weights 77 x 10(6) to 182 x 10(6) as determined by electron microscopy. one strain contained only a smaller plasmid (50 x 10(6) daltons). several strains had both large and small (ca. 11 x 10(6) daltons) plasmids; one strain contained two large plasmids (112 x 10(6) and 136 x 10(6) ... | 1977 | 830650 |
| some features of mannitol metabolism in rhizobium japonicum. | | 1977 | 833572 |
| bacteriophage 7-7-1 adsorbs to the complex flagella of rhizobium lupini h13-3. | bacteriophage 7-7-1 is shown to adsorb specifically to the complex flagella of its host rhizobium lupini h13-3. deflagellation of motile cells before the addition of phage leads to a complete inhibition of phage propagation for at least 60 min. among phage-resistant mutants, many non-motile (mot) and non-flagellated (fla) derivatives of r. lupini h13-3 have been selected. electron microscopic observations indicate that bacteriophage 7-7-1 attaches with its short tail fibres to the conspicuous he ... | 1977 | 833579 |
| formation of merodiploid clones by cojugation in rhizobium lupini. | off the transconjugants formed in the r. lupini conjugation 0.5 to 5% are merodiploids. when two differently pigmented parents are used in the crossing experiment the diploid transconjugants by their additive pigmentation type. the segregation patterns of these diploid clones were analyzed. the results are in agreement with the theory that the exogenotic donor dna can be integrated at different sites of the homologous recipient chromosomal region forming a tandem sequence. consequently the segre ... | 1977 | 834178 |
| transfer of virulence in vivo and in vitro in agrobacterium. | | 1977 | 834308 |
| ti plasmids of agrobacterium as conjugative plasmids. | | 1977 | 834309 |
| binding of pea lectins to a glycan type polysaccharide in the cell walls of rhizobium leguminosarum. | | 1977 | 838048 |
| carotenoids of rhizobia. i. new carotenoids from rhizobium lupini. | | 1977 | 838596 |
| comparison of nucleic acid content in populations of free-living and symbiotic rhizobium meliloti by flow microfluorometry. | populations of symbiotic rhizobium meliloti extracted from alfalfa nodules were shown by flow microfluorometry to contain a significant number of bacteroids with higher nucleic acid content than the free-living rhizobia. bacteroids with lower nucleic acid content than the free-living bacteria were not detected in significant quantities in these populations. these results indicate that the incapability of bacteroids to reestablish growth in nutrient media may not be caused by a decrease in nuclei ... | 1977 | 838682 |
| nitrogen fixation in nitrate reductase-deficient mutants of cultured rhizobia. | forty-eight mutants unable to reduce nitrate were isolated from "cowpea" rhizobium sp. strain 32hl and examined for nitrogenase activity in culture. all but two of the mutants had nitrogenase activity comparable with the parental sttain and two nitrogenase-defective strains showed alterations in their symbiotic properties. one strain was unable to nodulate either macroptilium atropurpureum or vigna uguiculata and, with the other, nodules appeared promptly, but effective nitrogen fixation was del ... | 1977 | 838684 |
| induction of d-aldohexoside:cytochrome c oxidoreductase in agrobacterium tumefaciens. | d-aldohexopyranoside:cytochrome c oxidoreductase (aco) was strongly induced by cellobiose, alpha-methylglucoside, beta-methylglucoside, kojibiose, and sophorose. induction was rapid, and aco was readily detectable within 10 min after addition of cellobiose as inducer. although not measurable for 30 to 40 min after addition of inducer, once started, the rate of induction with alpha-methylglucoside equaled or even exceeded that obtained with cellobiose. induction by sucrose, maltose, alpha-alpha-t ... | 1977 | 838689 |
| a new amino acid derivative present in crown gall tumor tissue. | | 1977 | 843357 |
| comparison of thirty-seven strains of vd-3 bacteria with agrobacterium radiobacter: morphological and physiological observations. | thirty-seven cultures of vd-3 bacteria, isolated from clinical specimens, were characterized morphologically and physiologically. the cultures produced positive reactions when tested for oxidase, urease, nitrate reduction, phenylalanine deaminase, oxidative metabolism of carbohydrate substrates, and 3-ketolactose production. these peritrichously flagellated microorganisms were isolated primarily from the respiratory tract. when compared to authentic strains of agrobacterium, they appeared to be ... | 1977 | 845244 |
| demonstration of an octasaccharide repeating unit in the extracellular polysaccharide of rhizobium meliloti by sequential degradation. | | 1977 | 858877 |
| [competitive ability of strains of rhizobium lupini]. | | 1977 | 859459 |
| [detection of nitrogenase activity in a collection of rhizobium strains]. | | 1977 | 859462 |
| [comparative characteristics of several industrial strains of pea nodule bacteria]. | | 1977 | 865309 |
| [effect of inhibitors of transcription on the growth of mycoplasma and agrobacterium tumefaciens 8628]. | | 1977 | 865316 |
| [concentration of phosphoorganic compounds in vegetative and bacteroid cells of rhizobium lupini]. | | 1977 | 865320 |
| [avirulent mutants of agrobacterium tumefaciens 8628 resistant to alpha-amanitin]. | | 1977 | 865330 |
| resistance of rhizobium strains to phygon, spergon, and thiram. | strains of rhizobium meliloti, rhizobium sp. nodulating cowpeas, and r. phaseoli derived from cultures susceptible to tetramethylthiuram disulfide (thiram), 2,3-dichloro-1,4-naphthoquinone (phygon), and 2,3,5,6-tetrachloro-p-benzoquinone (spergon), respectively, grew in the presence of high concentrations of the fungicides and converted them to products not toxic to the sensitive rhizobia. the results of chemical assays demonstrated that the pesticides were destroyed by the resistant bacteria bu ... | 1977 | 869529 |
| [effect of humus and microbial inoculates on yield and nitrogen uptake by agricultural plants]. | the application of humus had a positive effect on grain and straw yield of paddy and the yield increased with the increasing concentration of humus. the highest dose ((0,05%) corresponding to 1120 kg humus/ha significantly increased the grain and straw yield by 85 and 30 per cent over control. the efficiency of algal inoculation was enhanced in the presence of humus and recorded 41 per cent increase in yield over algae. the nitrogen uptake was also appreciably increased by grain and straw due to ... | 1977 | 870800 |
| further evidence for the regulation of bacterial populations in soil by protozoa. | after the addition to soil of large numbers of a cowpea rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. when indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall initially, but its abundance declined to about 10(7)/g when actidione-resistant protozoa arose in significant numbers. the addition to actidione-treated soil of an antibiotic-resista ... | 1977 | 879960 |
| symbiotic effectiveness of antibiotic-resistant mutants of fast- and slow-growing strains of rhizobium nodulating lotus species. | mutants resistant ot 16 individual antibiotics were isolated from two fast-growing and two slow-growing strains of lotus rhizobia and their symbiotic effectiveness on lotus pedunculatus evaluated. resistance to streptomycin, spectinomycin, chloramphenicol, and tetracycline (inhibitors of protein synthesis) was associated with little or no loss of effectiveness with all four strains but resistance to nalidixic acid and rifampicin (inhibitors of nucleic acid synthesis), and to d-cycloserine, novob ... | 1977 | 890601 |
| stable incorporation of plasmid dna into higher plant cells: the molecular basis of crown gall tumorigenesis. | | 1977 | 890735 |
| absence of 3-ketolactose production by rhizobium phaseoli. | | 1977 | 892671 |
| zeatin ribonucleosides in the transfer ribonucleic acid of rhizobium leguminosarum, agrobacterium tumefaciens, corynebacterium fascians, and erwinia amylovora. | until recently, the presence in transfer ribonucleic acid (trna) of the hydroxylated cytokinin ribosylzeatin [n6-(4-hydroxy-3-methylbut-2-enyl)adenosine]was thought to be unique to higher plants. this extension of work from several laboratories indicates the presence of 2-methylthioribosylzeatin in the trna of the plant-associated bacteria rhizobium leguminosarum, agrobacterium tumefaciens, and corynebacterium fascians, but not in that of erwinia amylovora. this cytokinin has the cis configurati ... | 1977 | 893341 |
| [serologic properties of mutants of rhizobium trifolii and their interrelationship with effectiveness]. | uv and gamma irradiation of rhizobium trifolii, strains 347a and 311a, produced their mutants with changed antigenic properties and effectiveness. the extent of changes of antigens did not depend on the dose of irradiation because mutants that completely lost antigens typical of the parent strains were obtained at different doses of uv and gamma rays. no correlation was established between the antigenic properties of the mutants of rhizobium trifolii and their effectiveness. | 1977 | 895563 |
| citric acid cycle enzymes and nitrogenase in nodules of pisum sativum. | activity of isocitric dehydrogenase (isocitrate dehydrogenase (nadp+); ec 1.1.1.42) in bacteroids is highest at the time of maximum nitrogen fixation. it is likely that isocitric dehydrogenase is the source of reductant for dinitrogen fixation. | 1977 | 907916 |
| polarity in the exponential-phase rhizobium japonicum cell. | highly distinctive aspects of the exponentail-phase rhizobium japonicum cell were disclosed by means of thin sections, freeze etching, fluorescent antibodies, and ruthenium red staining. polarity was expressed in the form of reserve polymer distribution near one end of the cell and as cytoplasmic localization near the opposite end. in addition, exocellular polysaccharide (eps) accumulated preferentially around the cytoplasmic end, and the feature described previously as an "immunofluorescent pol ... | 1977 | 907920 |
| [change in phage sensitivity in rhizobium meliloti transformants]. | cultures of rhizobium meliloti were studied in order to test their phage resistance. seven variants of the modification of phage resistance were found among the transformants under study, and an attempt was made to interpret them. | 1977 | 909473 |
| [electrophoretic characteristics of the protein composition of mutant forms of pea nodular bacteria]. | a water-soluble protein fraction was studied by gel electrophoresis in the parent and mutant forms of the nodule bacterium rhizobium leguminosarum. the composition of this fraction changed under the action of ntg. changes in the composition of protein components in the mutants as compared with that of the parent strain are caused presumably by their modified specificity. | 1977 | 909477 |
| a restriction endonuclease from agrobacterium tumefaciens. | | 1977 | 913578 |
| homology between ti-plasmids of agrobacterium tumefaciens: hybridzation studies using electron microscopy [proceedings]. | | 1977 | 913837 |
| comparative study of ti-plasmids in agrobacterium tumefaciens by use of restriction enzymes [proceedings]. | | 1977 | 913838 |
| [a study of six cultures of "agrobacterium tumefaciens" and "a. radiobacter" (author's transl)]. | | 1977 | 921138 |
| the incorporation and expression of agrobacterium plasmid genes in crown gall tumors. | | 1977 | 921688 |
| lectins as determinants of specificity in legume-rhizobium symbiosis. | | 1977 | 921690 |
| genetic hydridization of root-nodule bacteria (rhizobium). | | 1977 | 921702 |
| genetic mapping of rhizobium meliloti using rp4. | | 1977 | 921703 |
| studies on agrobacterium tumefaciens. viii. avirulence induced by temperature and ethidium bromide. | when tumorigenic strains of agrobacterium tumefaciens were subcultured at temperatures between 31.5 and 37 degrees c or in broth containing ethidium bromide, they lost their capacity to induce tumors in tomato plants. the sensitivities of curing virulence (tumorigenicity) depended on the density of the population of cells, fewer cells (100/ml) being more sensitive to curing than higher densities (10(6)/ml). the loss of virulence need not require the total loss of the virulence-specifying plasmid ... | 1977 | 922605 |
| plasmid content and tumor initiation complementation by agrobacterium tumefaciens iibnv6. | avirulent strains iibnv6 and nt1, derived from virulent strains of agrobacterium tumefaciens, were tested for their ability to enhance tumor initiation (complement) on coinoculation with tumorigenic strains. strain nt1, cured of the agrobacterium virulence plasmid, failed to complement when inoculated with its virulent parental strain or with other virulent strains. strain iibnv6, however, complemented with all virulent strains tested. attachment to host wound sites by both strain iibnv6 and the ... | 1977 | 924973 |
| intergeneric transfer of genes involved in the rhizobium-legume symbiosis. | genes that seem to be involved in the initial steps of infection of a legume by rhizobium have been transferred, by transformation, to mutant strains of azotobacter vinelandii that are unable to fix nitrogen. these genes code for a surface antigen that binds specifically to a protein from the host plant. | 1977 | 929179 |
| regulation of symbiotic nitrogen fixation in root nodules of alfalfa (medicago sativa) infected with rhizobium meliloti. | symbiotic nitrogen fixation of rhizobium meliloti bacteroids in medicago sativa root nodules was suppressed by several inorganic nitrogen sources. amino acids like glutamine, glutamic acid and aspartic acid, which can serve as sole nitrogen sources for the unnodulated plant did not influence nitrogenase activity of effective nodules, even at high concetrations. ammonia and nitrate suppressed symbiotic nitrogen fixation in vivo only at concentrations much higher than those needed for suppression ... | 1977 | 931507 |
| oxygen requirement for acetylene reduction by pure cultures of rhizobia. | the oxygen and nutritional requirements for acetylene reduction by rhizobium japonicum and rhizobium sp. in liquid culture are described. the optimal oxygen concentration was about 0.1% in the gas phase, which is lower than that of any other known aerobic nitrogen-fixing microorganism. these organisms are also unique in that nitrogenase synthesis is not repressed in the presence of ammonium chloride under certain cultural conditions, in contrast to other wild-type bacteria. | 1976 | 931945 |
| octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of agrobacterium tumefaciens. | several nopaline degrading strains and one octopine degrading strain are shown to loose oncogenicity as well as the ability to utilize these guanidine compounds when they are cured of their ti plasmid. to investigate whether the specific genes involved in the utilization of one or the other compound are located on the plasmid, plasmid-transfer experiments have been performed. the plasmid from a nopaline degrading strain has been transferred to a naturally non oncogenic agrobacterium namely a. ra ... | 1976 | 934053 |
| occurrence of trans-ribosylzeatin in agrobacterium tumefaciens trna. | | 1976 | 934334 |
| mixed inoculations with effective and ineffective strains of rhizobium leguminosarum. | | 1976 | 939736 |
| [the effect of nitrate and nitrite on the respiration and cytochrome system of rhizobium lupini]. | differential spectrophotometry and oxygen electrode were used to study the effect of nitrate and nitrite on the cytochrome system and respiration of rhizobium lupini cultivated in the conditions of different aeration, and bacteroids of this strain isolated from lupine nodules. in the anaerobic conditions, nitrate (10(-3) m) accepts electrons from the cytochrome system in suspensions of bacteroids and bacterial cells grown in the microaerophilic conditions (12 mum o2), but does not oxidize cytoch ... | 1976 | 940501 |
| host-symbiont interactions. i. the lectins of legumes interact with the o-antigen-containing lipopolysaccharides of their symbiont rhizobia. | | 1976 | 945740 |