| failure to isolate brucella abortus from embryos or ova from culture-positive superovulated cows. | nine, brucella abortus culture positive 2-yr-old cows were used to test the hypothesis that embryos and ova collected from such cows are not infected. superovulation was induced at varying times postpartum or postabortion with intramuscular injections of follicle stimulating hormone (fsh). the cows were artificially inseminated with b. abortus-negative semen. superovulations and nonsurgical embryo collections nonsurgical embryo collections were attempted twice for each cow. jugular blood, udder ... | 1988 | 16726359 |
| immune responses of elk to initial and booster vaccinations with brucella abortus strain rb51 or 19. | previous studies have suggested that currently available brucellosis vaccines induce poor or no protection in elk (cervus elaphus nelsoni). in this study, we characterized the immunologic responses of elk after initial or booster vaccination with brucella abortus strains rb51 (srb51) and 19 (s19). elk were vaccinated with saline or 10(10) cfu of srb51 or s19 (n=seven animals/treatment) and booster vaccinated with a similar dosage of the autologous vaccine at 65 weeks. compared to nonvaccinates, ... | 2006 | 17028213 |
| rescuing valuable genomes by animal cloning: a case for natural disease resistance in cattle. | tissue banking and animal cloning represent a powerful tool for conserving and regenerating valuable animal genomes. here we report an example involving cattle and the rescue of a genome affording natural disease resistance. during the course of a 2-decade study involving the phenotypic and genotypic analysis for the functional and genetic basis of natural disease resistance against bovine brucellosis, a foundation sire was identified and confirmed to be genetically resistant to brucella abortus ... | 2007 | 17179549 |
| a survey of brucellosis and tuberculosis in bison in and around wood buffalo national park, canada. | examinations of complete or partial remains of 72 bison found dead in and around wood buffalo national park, canada, revealed evidence of brucellosis in 18 (25%) and tuberculosis in 15 (21%), with a combined prevalence of 42%. urease-positive and ureasenegative strains of brucella abortus biovar 1, and strains of biovar 2, were isolated from tissues of bison, including synovium and exudate from severe arthritic lesions. mycobacterium bovis was isolated from a range of granulomatous lesions that ... | 1990 | 17423532 |
| phenotypic and genotypic characterisation of brucella strains isolated from cattle in the gambia. | thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the western region of the gambia in order to isolate and characterise brucella species. information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. twenty-six (74 per cent) of the serum samples were positive in the rose bengal ... | 2010 | 20543166 |
| seroprevalence of bovine brucellosis in trade cattle slaughtered in ibadan, nigeria, from 2004-2006. | a seroprevalence study was carried out among trade cattle slaughtered at bodija municipal abattoir, ibadan (southwestern nigeria) over a period of 3 consecutive years from 2004 to 2006 with a view to determining the breed, sex and age distribution in the seropositivity of bovine brucellosis. in total, 1642 animals were examined for antibodies to brucella abortus using the rose bengal test. seroprevalences of 6.00%, 6.17% and 5.31% were obtained in the years 2004, 2005 and 2006, respectively but ... | 2010 | 20649155 |
| heat-killed and γ-irradiated brucella strain rb51 stimulates enhanced dendritic cell activation, but not function compared with the virulent smooth strain 2308. | brucella spp. are gram-negative, facultative intracellular bacterial pathogens that cause abortion in livestock and undulant fever in humans worldwide. brucella abortus strain 2308 is a pathogenic strain that affects cattle and humans. currently, there are no efficacious human vaccines available. however, b. abortus strain rb51, which is approved by the usda, is a live-attenuated rough vaccine against bovine brucellosis. live strain rb51 induces protection via cd4(+) and cd8(+) t-cell-mediated i ... | 2010 | 20812951 |
| expression of mpb83 from mycobacterium bovis in brucella abortus s19 induces specific cellular immune response against the recombinant antigen in balb/c mice. | immunodominant mpb83 antigen from mycobacterium bovis was expressed as a chimeric protein fused to either β-galactosidase, outer membrane lipoprotein omp19 or periplasmic protein bp26 in gram-negative brucella abortus s19, in all cases driven by each gene's own promoter. all fusion proteins were successfully expressed and localized in the expected subcellular fraction. moreover, omp19-mpb83 was processed as a lipoprotein when expressed in b. abortus. splenocytes from balb/c mice immunized with t ... | 2010 | 20888425 |
| comparative analysis of the early transcriptome of brucella abortus - infected monocyte-derived macrophages from cattle naturally resistant or susceptible to brucellosis. | brucellosis is a worldwide zoonotic infectious disease that has a significant economic impact on animal production and human public health. we characterized the gene expression profile of b. abortus-infected monocyte-derived macrophages (mdms) from naïve cattle naturally resistant (r) or susceptible (s) to brucellosis using a cdna microarray technology. our data indicate that (1) b. abortus induced a slightly increased genome activation in r mdms and a down-regulated transcriptome in s mdms, dur ... | 2010 | 20932540 |
| iron homeostasis in brucella abortus: the role of bacterioferritin. | brucella abortus is the etiological agent of bovine brucellosis, an infectious disease of humans and cattle. its pathogenesis is mainly based on its ability to survive and multiply inside macrophages. it has been demonstrated that if b. abortus ferrochelatase cannot incorporate iron into protoporphyrin ix to synthesize heme, the intracellular replication and virulence in mice is highly attenuated. therefore, it can be hypothesized that the unavailability of iron could lead to the same attenuatio ... | 2010 | 21046346 |
| serological evidence of brucella abortus prevalence in punjab province, pakistan--a cross-sectional study. | twenty-one villages in punjab province, seven from each region (north, central& south) were randomly selected to determine the prevalence of b. abortus infection by two age categories (group a: 0–2 years; group b: >2 years) in cattle and buffalo populations. in each village, eight blood samples were collected from each age group of cattle and buffaloes. sera from a total of 672 blood samples (336 each from cattle and buffaloes) were analysed for the presence of antibodies to b. abortus using ros ... | 2010 | 21117286 |
| a genetic comparison of brucella abortus isolates from animals and humans by using an mlva assay. | the mlva assay is known to have a high ability to identify and discriminate brucella species, so that it can be used as an epidemiological tool to discriminate brucella isolates originating from restricted geographic sources. in this study, the genetic profiles of 38 b. abortus isolates from humans were analyzed and compared with genotypes from animal isolates in south korea. as a result, it was found that they did not show high genetic diversity and were compacted. they were clustered together ... | 2010 | 21193833 |
| effect of polymorphisms in the slc11a1 coding region on resistance to brucellosis by macrophages in vitro and after challenge in two bos breeds (blanco orejinegro and zebu). | the resistance/susceptibility of selected cattle breeds to brucellosis was evaluated in an f1 population generated by crossing animals classified as resistant (r) and susceptible (s) (r x r, r x s, s x r, s x s) based on challenges in vitro and in vivo. the association between single nucleotide polymorphisms identified in the coding region of the slc11a1 gene and resistance/susceptibility was estimated. the trait resistance or susceptibility to brucellosis, evaluated by a challenge in vitro, sho ... | 2010 | 21637417 |
| reply to letter to the editor by blasco and moriyon (2009) concerning the manuscript "eradication of bovine brucellosis (bb) in the azores, portugal-outcome of a 5-year programme (2002-2007) based on test-and-slaughter and rb51 vaccination" by h. martins et al. (2009). | | 2010 | 20079547 |
| bovine brucellosis. | infection of cattle caused by brucella abortus (ie, bovine brucellosis) has been of political importance in the united states for many decades. the most common clinical manifestation of brucellosis in natural hosts is reproductive loss resulting from abortion, birth of weak offspring, or infertility. brucellosis regulatory programs were primarily developed as the most efficient way to prevent human infections. this article discusses cattle vaccination with b abortus strains 19 and rb51. other re ... | 2010 | 20117540 |
| variable-number tandem repeat markers for identification of brucella abortus 82 and 75/79-av vaccine strains. | in russia, live vaccine strains brucella abortus 82 and b. abortus 75/79-av have been widely and extensively utilized for specific prophylaxis of cattle brucellosis. to differentiate these vaccine strains from each other and laboratory collection of other vaccine (n=4), reference (n=15) and field brucella strains (n=61), the multiple-locus variable-number tandem repeat (vntr) analyses (mlva) were used with 12 loci containing tandem repeats from 134bp to 8bp recently described for brucella spp. t ... | 2010 | 20362200 |
| comparative study of the immunobiological properties of live brucellosis vaccines. | findings from the comparative study of the immunobiological properties of live brucellosis vaccines in guinea pigs are presented in the article. vaccines from strains brucella abortus 19 (u.s.) and 82 (russia), in the s and sr forms, respectively, exhibited the highest and most pronounced immunological efficacy, while vaccines from strains b. abortus 82-ps (in the rs form) and b. abortus rb-51 and 75/79-ab (in the r forms) exhibited the lowest. the live vaccine from strain b. abortus 82, togethe ... | 2010 | 20362201 |
| designing vaccines against cattle brucellosis. | the article presents the results from a study of two new vaccines against cattle brucellosis from brucella abortus cultures. one of these is a live dry vaccine prepared from a weakly agglutinogenic strain in the sr form and the second, an inactivated adjuvant vaccine from an non-agglutinogenic strain in the r form. the immunogenic properties of the vaccines were studied in three tests on heifers with infection by a virulent b. abortus culture. the vaccines passed the farm-scale testing and were ... | 2010 | 20362619 |
| development and characterization of a modified komarov's bullet for ballistic delivery of live brucella abortus strains 82 and 19 to cattle and bison. | in this study a modified komarov's bullet was developed to remotely deliver live brucellosis vaccines (brucella abortus 82 and brucella abortus 19). after modification, the bullet payload could carry the desired dose (10(11)cfu) of vaccine. as the bullet components were toxic to the live bacteria, a special protective coating was developed for the bullet inner surface that maintained vaccine viability. vaccine viability was not influenced by ballistic delivery and the characteristics of the modi ... | 2010 | 20362625 |
| the development of a selective medium for the brucella abortus strains and its comparison with the currently recommended and used medium. | the brucella spp. are fastidious and relatively slow-growing organisms. the isolation of such strains in a variety of specimens often requires the use of a selective medium to reduce or eliminate the growth of unexpected microorganisms. the modified brucella selective (mbs) medium, which contains improved antibiotic mixtures, erythritol as the only carbon source, and neutral red as a ph indicator, showed good selectivity for the brucella abortus strains, including the rb51 vaccine strain. erythr ... | 2010 | 20385349 |
| epidemiological study of bovine brucellosis in three agro-ecological areas of central oromiya, ethiopia. | a cross-sectional sero-epidemiological study of bovine brucellosis was conducted between september 2005 and march 2006 in three separate agroecological areas of central oromiya, ethiopia. in this study, a total of 176 clusters (farms) and 1,238 animals were selected, using the one-stage cluster sampling method. fifty-nine clusters and 423 animals were selected from the lowland areas; 58 clusters and 385 animals from the midland areas and 59 clusters and 430 animals from the highlands. serum samp ... | 2009 | 20462151 |