| effects of immunization with bovine herpesvirus-1 glycoproteins on bovine herpesvirus-1-induced alteration of bovine neutrophil chemotactic and anti-pasteurella haemolytica activities. | it has been reported previously that active bovine herpesvirus-1 (bhv-1) infection greatly enhances the susceptibility of cattle to secondary bacterial pneumonia involving pasteurella haemolytica. the present study examines the possibility that immunization of bhv-1 naive calves with purified bhv-1 glycoproteins would protect them against changes in neutrophil function that might compromise their ability to eliminate p. haemolytica during an active bhv-1 infection. the results show that circulat ... | 1988 | 2848379 |
| the effect of substrate on the production of infectious virus by cells in culture. | herpes simplex virus type i (hsv-1), infectious bovine rhinotracheitis virus (ibr) and turkey herpesvirus were examined for growth in cells cultured on three different substrates. the substrates were glass, deae-dextran and collagen gel. with two of the viruses, hsv-1 and ibr, there were no apparent differences in production as a function of substrate. in contrast, the amount of the turkey herpesvirus which was recovered varied greatly with the substrate. titers were highest on glass, followed b ... | 1988 | 2848844 |
| detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity. | a procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. wheat germ lectin, limulus polyphemus lectin and concanavalin a, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. these lectins have also been used for affinity-purification of sets of glycoproteins. the procedure is easy, rapid, cheap and, when biotin is used, non-radioactive. | 1988 | 2848857 |
| [serologic survey of rinderpest, ibr-ipv, rsb, pi3 and bvd-md viruses in cattle in shaba and western zaire]. | | 1988 | 2851152 |
| changing trends in infectious bovine rhinotracheitis in great britain. | the incidence and severity of infectious bovine rhinotracheitis in great britain increased markedly in the mid to late 1970s. data available in maff records have been used to analyse the changing trends in disease incidence and serological prevalence, with particular emphasis on the most recent decade. | 1988 | 2851204 |
| kinetics of large-scale production of bovine leukocyte interferon, using three viral inducers. | kinetics of large-scale production of naturally derived bovine leukocyte interferon (ifn) was investigated using sendai virus, newcastle disease virus, and infectious bovine rhinotracheitis virus inducers. cultures were tested for ifn production every 6 hours for 66 hours. the effect of varying the priming dose of sendai virus from 0 to 50% of total virus dose and the effect of varying the priming time from 0 to 4 hours before induction also were investigated. other factors explored were effects ... | 1988 | 2851950 |
| effect of respiratory infections caused by bovine herpesvirus-1 or parainfluenza-3 virus on bovine alveolar macrophage functions. | calves, 90 to 130 days old, were inoculated with bovine herpesvirus-1 (bhv-1) or parainfluenza-3 (pi-3) virus. pulmonary lavage specimens obtained from calves before virus inoculation contained 98% alveolar macrophages (am) and 1% neutrophils. six days after inoculation, the mean percentage of neutrophils in lavage specimens had significantly increased to 7.9 +/- 6.0% in bhv-1-inoculated calves and to 18.3 +/- 9.9% in pi-3 virus-inoculated calves, reflecting viral-induced pulmonary inflammation ... | 1988 | 2851951 |
| further biological, serological and biochemical characterization of north american, european and southeast asian strains of bovine herpesvirus 1 compared with other alphaherpesvirinae members. | hemagglutination activity, structural protein profiles and neutralization assays were used in a comparative study of bovine herpesvirus 1 strains from the u.s.a., canada, great britain, denmark and malaysia with equine, feline and human herpesviruses in order to further characterize the bovine herpesvirus 1 hemagglutinin. bovine herpesvirus 1 strains of different geographical origins all showed hemagglutinating activity for mouse erythrocytes; furthermore, feline herpesvirus 1 was also shown to ... | 1988 | 2852870 |
| effect of prostaglandins pge2 and pgf alpha 2 on the mean plaque size of bovine herpesvirus 1. | the effect of prostaglandins pge2 and pgf alpha 2 on infectious bovine rhinotracheitis (ibr) virus (bovine herpesvirus 1; bhv-1) was studied by using the measurement of the mean size of plaques produced in cell culture by ibr/cu5 strain. three concentrations (0.1, 1 and 10 micrograms/ml) of prostaglandins, pge2 and pgf alpha 2 were used. each prostaglandin provoked an increase in the mean plaque size when compared to control plaques, at the concentrations 1 and 10 micrograms/ml for pge2 and 10 m ... | 1988 | 2852912 |
| studies on the in vitro biological activities of recombinant bovine tumor necrosis factor (rbotnf) alpha. i. synergistic antiviral efficacy of rbotnf alpha, recombinant bovine interferon gamma (rboifn gamma) and their combination. | the recent demonstration of the antiviral activity of recombinant human tnf has launched an interest in the use of tnf alpha for antiviral therapy in veterinary medicine. in the precent report, we demonstrate that pretreatment of bovine cells with rbotnf alpha reduces the yield of bovine herpesvirus type-1 (bhv-1) from infected cells. reduction in yield was similar to that observed in the presence of rboifn gamma. similarly, rbotnf alpha was able to protect bovine cells from virus-induced cytopa ... | 1988 | 2852921 |
| enhancement of infectious bovine keratoconjunctivitis by modified-live infectious bovine rhinotracheitis virus vaccine. | the effects of a modified-live infectious bovine rhinotracheitis virus vaccine (administered ocularly or intranasally) on experimentally induced infectious bovine keratoconjunctivitis were evaluated. the modified-live infectious bovine rhinotracheitis virus vaccine was administered to 13 male holstein calves (intranasally in 4 and ocularly in 9; day 0). five calves were not vaccinated and served as controls. calves were examined daily and, starting on day 4, moraxella bovis was administered ocul ... | 1988 | 2854704 |
| preincubation of bovine blood neutrophils with bovine herpesvirus-1 does not impair neutrophil interaction with pasteurella haemolytica a1 in vitro. | in this study we examined the direct effects of bovine herpesvirus-1 on the interaction of bovine blood neutrophils with pasteurella haemolytica a1. preincubation of neutrophils for approximately 2 h in vitro with bhv-1 at a multiplicity of infection of 5:1 had no effect on neutrophil random migration and directed migration to zymosan-activated bovine serum. neutrophils also were unimpaired in their ability to ingest and kill p. haemolytica a1. preincubation of neutrophils with bhv-1 did not eli ... | 1988 | 2855289 |
| generation of il-2 dependent bovine cytotoxic t lymphocyte clones reactive against bhv-1 infected target cells: loss of genetic restriction and virus specificity. | to gain insight into the mechanisms of immunity to bovine herpesvirus type-1 (bhv-1) in particular the importance of the t-cell response, we attempted to clone bovine cytotoxic t lymphocytes that were specific for bhv-1 infected autologous target cells. a number of bovine t cell clones were generated by limiting dilution in the presence of bovine recombinant il-2 and bhv-1 infected target cells as feeder layers. these clones were maintained in culture on crude il-2 containing supernatants. in fu ... | 1987 | 2855701 |
| de novo initiation of specific cell-mediated immune responsiveness in chickens by transfer factor (specific immunity inducer) obtained from bovine colostrum and milk. | transfer factors (tf) were prepared from colostrum and milk of bovines previously immunized with antigens obtained from coccidioides immitis, infectious bovine rhinotracheitis virus, or from the viral agents responsible for avian newcastle disease, laryngotracheitis disease or infectious bursal disease. the ability of bovine tf to transfer specific cell-mediated immune responsiveness to a markedly xenogenic species was studied using specific pathogen free (spf) and standard commercial (sc) chick ... | 1988 | 2897772 |
| rapid detection of bovine herpesvirus type 1 antigens in nasal swab specimens with an antigen capture enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of bovine herpesvirus type 1 (bhv-1) antigens in nasal swab specimens collected from infected animals. development of the elisa involved screening and selection of bhv-1-specific monoclonal antibodies for their ability to capture bhv-1 antigens and for their stability and activity after conjugation to horseradish peroxidase. forty combinations of capture-conjugate monoclonal antibody pairs were screened for detection of ... | 1985 | 2984246 |
| a single dilution enzyme-linked immunosorbent assay for the quantitative detection of antibodies to bovine herpesvirus type 1. | three serological assays were compared for detection of antibodies to bovine herpes-virus type 1. these were virus neutralization (vn), enhanced complement fixation (cf) and enzyme-linked immunosorbent assay (elisa). the elisa was developed using an infected cell lysate antigen and purified virus and was optimized in relation to antigen and antisera dilutions. the cf assay was enhanced by the addition of bovine complement. these 3 assays were compared for detection of: specific virus antibody ti ... | 1985 | 2984835 |
| ovarian lesions in heifers exposed to infectious bovine rhinotracheitis virus by non-genital routes on the day after breeding. | twelve heifers were exposed to either a colorado infectious bovine rhinotracheitis (ibr) virus isolate or an iowa ibr isolate obtained from a bovine respiratory disease outbreak. all inoculations were made on the day after the heifers had been in estrus and bred by an ibr virus-negative bull. pairs of heifers were inoculated with each virus isolate intravenously, intramuscularly or exposed by aerosol. the heifers were killed 11-15 days after inoculation and their reproductive tracts and ovaries ... | 1985 | 2984837 |
| bovine herpesvirus 1: differentiation of ibr- and ipv-viruses and identification and functional role of their major immunogenic components. | infectious bovine rhinotracheitis (ibr) and infectious pustular vulvovaginitis (ipv) virus strains of bovine herpesvirus 1 (bhv-1) can be differentiated by restriction endonuclease digestion of their dnas. antigens and polypeptide patterns of isolates of these different clinical entities are almost identical. page analysis of immunoprecipitates revealed three major immunogenic components in bhv-1 infected cells. these are glycoproteins with apparent molecular weights of 93,000 (gp93), 74,000 (gp ... | 1985 | 2985034 |
| effect of maternal antibody upon vaccination with infectious bovine rhinotracheitis and bovine virus diarrhea vaccines. | this report presents the normal rate of decay of maternal antibody and the influence of maternal antibody on responses to a single vaccination with modified-live bovine virus diarrhea and infectious bovine rhinotracheitis virus vaccines at 196 days of age and on response to vaccinations with the same vaccines given twice at 84 and 196 days of age. passive immunity decreased to near zero over the first six months of life for both bovine virus diarrhea and infectious bovine rhinotracheitis control ... | 1985 | 2985214 |
| protection from parainfluenza-3 virus and persistence of infectious bovine rhinotracheitis virus in sheep vaccinated with a modified live ibr-pi-3 vaccine. | ewes (n = 7) and their lambs (n = 12) were vaccinated with a commercial modified live infectious bovine rhinotracheitis-parainfluenza type 3 virus vaccine. both the vaccinated ewes and lambs and a group of unvaccinated ewes (n = 8) and their lambs (n = 13) were subsequently challenged with virulent parainfluenza type 3 virus. although absolute immunity to infection and clinical response was not conferred, the clinical response was less severe in vaccinated lambs. vaccinated animals also shed par ... | 1985 | 2985216 |
| immune response to bovine herpes herpesvirus type 1 infections: virus-specific antibodies in sera from infected animals. | the virus specificity of antibodies against bovine herpes virus type 1 was determined with a radioimmunoprecipitation assay and serum collected from natural and experimentally induced infections. by using sequentially collected sera, the development of antibodies to 4 to 5 viral glycoproteins and 11 to 12 nonglycosylated proteins was followed for the first 50 days after infection. the major and most consistent responses in experimentally and naturally infected animals were to four glycoproteins ... | 1985 | 2985645 |
| inability to detect a k cell in bovine peripheral blood leukocytes. | antibody-dependent cellular cytotoxicity to viral-infected cells, chicken red blood cells, and tumor cells was tested using different effector cell populations: polymorphonuclear cells, mononuclear cells, and mononuclear cells separated into adherent and nonadherent populations by sephadex g-10. polymorphonuclear cells were the most efficient mediators of antibody-dependent cellular cytotoxicity against most targets, although a combination of g-10 adherent and polymorphonuclear cells was more ef ... | 1985 | 2988185 |
| infection of early bovine embryos with bovine herpesvirus-1. | recently hatched bovine embryos were exposed in vitro to 1 of 4 strains of bovine herpesvirus-1 to determine whether the viruses would replicate in these embryos and, if so, what pathologic consequences would ensue. exposure to each of the viruses resulted in embryonic infection and death, and replication of the agents was demonstrated by electron microscopy and titration of progeny virus. there were no dramatic differences between virus strains in pathogenicity or in the ultrastructural patholo ... | 1985 | 2988378 |
| bovine herpesvirus-1 infection of cattle: kinetics of antibody formation after intranasal exposure and abortion induced by the virus. | the kinetics of antibody formation in holstein heifers after primary and secondary intranasal inoculation of bovine herpesvirus-1 (bhv-1) and after bhv-1-induced abortion was determined. sera were fractionated by gel filtration and ion-exchange chromatography. the antibody activity within serum immunoglobulin (ig) isotypes was assessed, using a plaque-reduction neutralization assay. the primary immune response to bhv-1 infection was characterized by the appearance of igm and igg antibodies in se ... | 1985 | 2990266 |
| kinetics of antibody formation after the reactivation of bovine herpesvirus-1 infection in cattle. | holstein heifers previously infected with bovine herpesvirus-1 were treated with corticosteroids to reactivate latent infection. the kinetics of serum immunoglobulin (ig) m, igg1, and igg2 antibody formation were determined. the secondary immune response which developed as a consequence of virus reactivation was characterized by an anamnestic igg antibody response. secondary igg1 and igg2 antibody formation constituted the igg antibody response. in addition, secondary igm antibody formation was ... | 1985 | 2990267 |
| effect of dexamethasone on the mean plaque size of bovine herpesvirus 1. | the effect of dexamethasone on infectious bovine rhinotracheitis virus (bovine herpesvirus 1: bhv 1) was studied by measuring the mean plaque size produced by eight virus strains under two concentrations of dexamethasone phosphate (0.1 and 1 mm). dexamethasone induced a significant reduction of the mean plaque size, whatever the strain used. some differences were noted between the bhv 1 strains studied, depending on the dexamethasone concentration. | 1985 | 2990317 |
| european isolates of bovine herpesvirus 1: a comparison of restriction endonuclease sites, polypeptides, and reactivity with monoclonal antibodies. | eleven european isolates of bovine herpesvirus 1 (bhv-1), together with two reference virus strains were compared by restriction endonuclease digestion, by sds-polyacrylamide gel electrophoresis (sds-page), and by their reactivity with a panel of monoclonal antibodies (mcab's). based on the cleavage pattern of viral dna with the restriction endonuclease hind iii the strains could be assigned to one of two established major virus types. analysis by sds-page of viral polypeptides revealed that fou ... | 1985 | 2990389 |
| pneumonia in calves produced with aerosols of pasteurella multocida alone and in combination with bovine herpesvirus 1. | pathological changes in respiratory tracts were studied in 30 calves following exposure to aerosols of pasteurella multocida or to bovine herpesvirus 1 and p. multocida. two groups of five calves were exposed to aerosols of one of two types of p. multocida only, which produced lobar pneumonia in one calf of each group. another five groups of four calves were exposed to aerosols of bovine herpesvirus 1 and four to seven days later to one of the two types or one sub-type of p. multocida. extensive ... | 1985 | 2990648 |
| sensitivity and specificity of an enzyme-linked immunosorbent assay for the detection of infectious bovine rhinotracheitis viral antibody in cattle. | an enzyme-linked immunosorbent assay was developed to detect bovine serum antibody to infectious bovine rhinotracheitis virus. the specificity of this assay in 304 bovine sera, collected from an infectious bovine rhinotracheitis virus-free herd, was 100%; in sera from 62 cattle inoculated with an intranasal vaccine, its diagnostic sensitivity was 27.4% at one month and 100% at six months, postvaccination. in 303 bovine sera with standard serum neutralizing antibody titers of greater than or equa ... | 1985 | 2990649 |
| a comparison of the genomes of bovine herpesvirus type 1 and pseudorabies virus. | the dna sequence homology between bovine herpesvirus type 1 (bhv-1) and pseudorabies virus (prv) was examined. reciprocal cross-hybridization of viral dna labelled by nick translation to southern blots of restriction endonuclease-digested dna detected homologous sequences dispersed throughout the genomes of the two viruses. the dna-dna hybrids formed were stable under high-stringency wash conditions. sequences of a 32p-labelled prv dna fragment probe were found to hybridize only to a specific re ... | 1985 | 2991445 |
| effect of human leukocyte a interferon on prevention of infectious bovine rhinotracheitis virus infection of cattle. | human leukocyte a interferon was evaluated for its ability to prevent infectious bovine rhinotracheitis virus-induced respiratory tract disease in cattle. weanling calves were treated daily for 1 week with 50 x 10(6) u of interferon, intranasally (by nebulization) and im, and inoculated with infectious bovine rhinotracheitis virus on the first day of treatment. respiratory tract disease was less severe in treated as compared with nontreated calves which were given only infectious bovine rhinotra ... | 1985 | 2992320 |
| effect of primary and recurrent infections bovine rhinotracheitis virus infection on the bovine ovary. | six heifers were inoculated iv at estrus with the iowa or colorado isolates of infectious bovine rhinotracheitis virus (ibrv). subsequent measurements of plasma progesterone indicated that corpus luteum function was depressed in all heifers. in the 1st estrous cycle after inoculation, progesterone values did not exceed 2 ng/ml in 3 heifers given the iowa isolate. although maximal progesterone values were greater than or equal to 2 ng/ml in 3 heifers given the colorado isolate, values were lower ... | 1985 | 2992325 |
| establishment of a statistical base for use of elisa in diagnostic serology for infectious bovine rhinotracheitis. | the critical statistical parameters of an enzyme-linked immunosorbent assay were determined to enable quantitation of antibody responses in cattle affected with infectious bovine rhinotracheitis. a system of controlling well-to-well variations in optical density reading across a microtitre plate was evolved and dose--response assays were carried out to determine the dilution of serum which gave the greatest discrimination between acute and convalescent sera from an infected animal. use of a stan ... | 1985 | 2993311 |
| reaction of goats to infection with infectious bovine rhinotracheitis virus. | intranasal exposure of goats to infectious bovine rhinotracheitis virus resulted in mild respiratory disease and virus reisolation from nasal secretions. no disease was produced in goats exposed to the same virus by the genital or ocular routes. there was serological evidence of contact transmission of infection from infected goats to cattle. virus recrudescence was not detected in goats treated with dexamethasone two months after virus inoculation. | 1985 | 2994191 |
| excretion and reexcretion of thermosensitive and wild-type strains of infectious bovine rhinotracheitis virus after co-infection or two successive infections. | twelve cattle were divided into 2 groups. the first was intranasally co-infected with 2 strains of infectious bovine rhinotracheitis virus (bovine herpesvirus 1; bhv 1): the thermosensitive vaccine strain ibr/ts rlb106 and a belgian field isolate ibr/cu5. reactivation of bhv 1 was induced by dexamethasone treatment 2 months later and again 5 months later for 3 animals that only reexcreted small quantities of virus during the first dexamethasone treatment. the second group was intranasally infect ... | 1985 | 2994280 |
| thymidine kinase-negative bovine herpesvirus type 1 mutant is stable and highly attenuated in calves. | calves were vaccinated intranasally (in) or intravenously (iv) with a thymidine kinase-negative (tk-) bhv-1 mutant. vaccinated calves developed neutralizing antibodies but did not show clinical signs of infectious bovine rhinotracheitis (ibr). vaccination also prevented clinical signs of ibr disease following in challenge exposure of the calves to parental los angeles (la) and usda cooper strains of tk+ bhv-1. nasal swabs were collected for 10 days after the vaccination and the challenge exposur ... | 1985 | 2994602 |
| comparison of interferon production in cattle after intranasal infection with parainfluenza-3 live vaccine and avirulent ibr/ipv-herpesvirus. | | 1985 | 2996264 |
| ovarian lesions induced in heifers by intravenous inoculation with modified-live infectious bovine rhinotracheitis virus on the day after breeding. | four commercially available vaccine strains of modified-live infectious bovine rhinotracheitis virus were passaged once in embryonic bovine kidney cells. heifers were inoculated iv on the day after breeding with 5.0 ml of nondiluted cell culture fluid of each of the 4 strains. virus was reisolated from nasal swabs and blood collected during the week after inoculation. the heifers were killed 9 to 14 days after inoculation. mild-to-marked inflammatory and necrotic lesions were seen in the corpora ... | 1985 | 2996392 |
| [determination of virus-specific antibodies against the infectious bovine rhinotracheitis/infectious pustular vulvovaginitis virus in cattle sera using elisa and the serum neutralization test]. | | 1985 | 2996456 |
| effect of bovine alpha 1 interferon on bovine herpesvirus type 1-induced respiratory disease. | treatment of calves with bovine recombinant alpha 1 interferon prior to challenge with bovine herpesvirus type 1 increased the animals' ability to withstand a subsequent pasteurella haemolytica challenge. the reduction in viral-bacterial synergy observed following interferon treatment did not appear to be due to a direct effect of the interferon on virus replication in the upper respiratory tract. thus, even though interferon-treated animals shed slightly less virus from their nasal passages tha ... | 1985 | 2997372 |
| effect of tunicamycin and monensin on biosynthesis, transport, and maturation of bovine herpesvirus type-1 glycoproteins. | the effect of tunicamycin and monensin on the biosynthesis, intracellular transport, and maturation of bovine herpesvirus type-1 (bhv-1) glycoproteins was examined. tunicamycin completely inhibited the production of infectious virus particles and significantly reduced the incorporation of [3h]glucosamine into viral glycoproteins. in the presence of monensin, reduced amounts of infectious virus particles were produced, which was mainly due to inhibition of virus release, rather than virus product ... | 1985 | 2997997 |
| comparison of the herpesviruses of cattle by dna restriction endonuclease analysis and serologic analysis. | reference strains and field isolates of herpesviruses recovered from cattle in the united states were compared by restriction endonuclease (re) analysis and the indirect fluorescent antibody test. as a result of these comparisons, 5 major biotypes of bovine herpesvirus (bhv) were defined. these types were (i) infectious bovine rhinotracheitis virus (bhv-1), (ii) bovine herpes mammillitis virus (bhv-2), (iii) malignant catarrhal fever (mcf) virus (herpesvirus alcelaphinae), (iv) the group of slow ... | 1985 | 2998238 |
| prevalence of antibodies to bovid herpesvirus 1 (ibr-ipv), bovine virus diarrhoea, bovine respiratory syncytial parainfluenza 3, adeno a and adeno b viruses in indigenous and imported moroccan cattle. | a serological survey on prevalence of antibodies to bovid herpesvirus 1 (ibr-ipv) bovine virus diarrhoea, bovine respiratory syncytial, parainfluenza 3, adeno a and b viruses was performed in 524 cattle from different areas and management conditions in morocco. general antibody prevalence was 62.8, 48.5, 70.4, 68.1, 9.0 and 12.4, for the six viruses, respectively. higher prevalence and high antibody titers to most of the investigated viruses were found among cattle from extensive management syst ... | 1985 | 2998260 |
| diagnosis and prophylaxis of infectious bovine rhinotracheitis: the role of virus latency. | efficient methods of diagnosis and prophylaxis of infectious bovine rhinotracheitis must consider the concept of latency of the etiological agent, infectious bovine rhinotracheitis virus (bovine herpesvirus 1; bhv 1). the identification of bhv 1 in nasal mucus samples or a rise in specific antibodies have to be cautiously interpreted, because they can signify either a primary infection or a reexcretion of the virus after reactivation. the isolated virus can also either be a vaccine or a virulent ... | 1985 | 2998697 |
| in vitro protective effect of bacteria-derived bovine alpha interferon i1 against selected bovine viruses. | we used bacteria-derived bovine alpha-interferon i1 (bo ifn-alpha i1) to study its antiviral effect in a bovine turbinate cell line on bovine diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and pseudorabies virus. we based our study upon replicate tests for each strain by using a block titration system with various concentrations of bo ifn-alpha i1 against various concentrations of virus. the data were compiled in two-axis tables (replicate x concentration) and we ... | 1985 | 2999188 |
| detection of bovine herpesvirus 1 dna immobilized on nitrocellulose by hybridization with biotinylated dna probes. | a molecular hybridization technique using biotinylated dna probes was used to detect bovine herpesvirus 1 (bhv-1) nucleic acid species immobilized on nitrocellulose. seventeen recombinant plasmids containing hindiii restriction fragments of the bhv-1 genome were compared for their ability to detect immobilized bhv-1 dna from purified virus and infected cells. one probe, pcb2, labeled by nick translation with either 3h or biotin, detected as little as 10 pg of viral dna. in time course experiment ... | 1985 | 2999191 |
| restriction endonuclease analysis of bovine herpesvirus 1 dna and nucleic acid homology between isolates. | isolates of bovine herpesvirus 1 (bhv-1) are associated with a variety of clinical manifestations. to determine if a single form of bhv-1 was responsible for the different virus-associated diseases or whether subpopulations of various isolates produced different clinical symptoms, studies were initiated to examine the dna restriction enzyme patterns and nucleic acid homology between virus isolates from respiratory infections and other clinical syndromes. differences between the genomes of severa ... | 1985 | 2999321 |
| epizootiological survey of parainfluenza-3, reovirus-3, respiratory syncytial and infectious bovine rhinotracheitis viral antibodies in sheep and goat flocks in quebec. | a serological survey was conducted in an attempt to detect antibodies against bovine respiratory viruses in sheep and goats from seven geographical areas of quebec. sera from 10% of the animals in 182 sheep flocks and 40 goat flocks were collected and specific antibodies against parainfluenza-3, reovirus type 3, respiratory syncytial and infectious bovine rhinotracheitis viruses were detected by hemagglutination-inhibition tests for the former viruses and complement fixation and seroneutralizati ... | 1985 | 3000551 |
| alteration of alveolar macrophage functions after aerosol infection with bovine herpesvirus type 1. | calves were aerosol challenged with bovine herpesvirus type i, and bronchoalveolar cells were subsequently retrieved by lavage from days 1 to 8 postinfection. alveolar macrophages (am), which were depleted of contaminating cells, were characterized with respect to phenotypic markers and functional activities. in most aspects, the changes suggested a stimulation of the am. with variations in kinetics the percentage of am expressing an mhc ii antigen and fc (immunoglobulin g)-mediated phagocytosis ... | 1986 | 3000945 |
| experimentally induced pneumonic pasteurellosis: dose-response relationships and protection against natural reinfection in calves. | calves were inoculated intranasally with 2 x 10(6.2) tissue culture infective doses of infectious bovine rhinotracheitis virus, followed in 7 days by intratracheal inoculations with 1 of 4 challenge doses of pathogenic pasteurella haemolytica. severity and duration of the ensuing clinical signs of respiratory tract disease were correlated with the challenge dose of bacteria. calves given 1 x 10(6) colony-forming units (cfu) of bacteria did not develop reliable clinical evidence of disease, where ... | 1985 | 3002216 |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| molecular epidemiology and pathogenesis of ruminant herpesviruses including bovine, buffalo and caprine herpesviruses l and bovine encephalitis herpesvirus. | restriction endonuclease dna fingerprints of herpesviruses isolated from 3 unrelated epidemics of bovine encephalitis are similar to each other and totally different from bovine herpesvirus 1 (bhv1). herpesviruses, antigenically related to bhv1, isolated from goats and buffalo have distinct dna fingerprints. we propose that bovine encephalitis herpesvirus is prototypic of a new bovine herpesvirus type and that alpha herpes viruses from individual ruminant species are species specific. | 1985 | 3002311 |
| studies on bovine herpesviruses. part 1. isolation and characterization of viruses isolated from the genital tract of cattle. | herpesviruses, previously isolated from cattle (theodoridis, 1978), were further studied and provisionally placed in the bovid herpesvirus 4 (bhv-4) group. major differences were found between ibr-ipv (bhv-1) and bhv-4 virus strains. in mdbk cells, all bhv-4 strains started growing at the edges of the culture, the process progressing slowly until destruction of the cells was complete by the 10th day. bhv-4 strains failed to induce neutralizing antibodies in cattle, goats and rabbits. only the ad ... | 1985 | 3003650 |
| [excretion of ibr virus, especially in milk, in experimentally infected cows]. | | 1985 | 3003895 |
| propagation of infectious bovine rhinotracheitis (bovine herpes-1) virus in murine primary cell cultures. | virus synthesis in balb/c mouse lung and kidney primary cultures infected with infectious bovine rhinotracheitis (ibr) virus started between 6 and 8 hours after virus inoculation and reached a maximum titer of 5.5 log10 plaque forming units (pfu) at 48 hours postinfection (pi). cytopathic effect (cpe) in cell cultures occurred at 8-10 hours and over 90% of the cells had cpe by 48 to 72 hours pi. the bulk of the newly replicated virus (60-80%) was cell-associated as determined by plaque assay of ... | 1986 | 3004011 |
| bovine herpesvirus 1: molecular and antigenic characteristics of variant viruses isolated from calves with neurological disease. | this report presents data showing that several virus isolates recovered in argentina, mainly from calves with non-purulent meningo-encephalitis, represent a hitherto unrecognized antigenic variant of bhv-1. the following experimental approaches have been adopted to demonstrate both the unique features among and the relatedness with bhv-1 of these isolates: i) crossed serum neutralization test with rabbit immune sera, ii) analysis by sds-polyacrylamide gel electrophoresis of radio-labeled virus i ... | 1986 | 3004386 |
| characterization of envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immunological methods. | ten glycoproteins of molecular weights of 180,000, 150,000, 130,000, 115,000, 97,000, 77,000, 74,000, 64,000, 55,000, and 45,000 (designated as 180k, 150k, etc.) and a single nonglycosylated 107,000-molecular-weight (107k) protein were quantitatively removed from purified bovine herpesvirus 1 (bhv-1) virions by detergent treatment. immunoprecipitations with monospecific and monoclonal antibodies showed that three sets of coprecipitating glycoproteins, 180k/97k, 150k/77k, and 130k/74k/55k, were t ... | 1986 | 3005625 |
| short repeats cause heterogeneity at genomic terminus of bovine herpesvirus 1. | analysis of the genomes of different bovine herpesvirus 1 strains revealed a ul terminal hindiii fragment differing in size (from 2.4 to 2.8 kilobases). this fragment polymorphism occurred in the dna of a wild-type isolate, in highly passaged, apathogenic tissue culture derivatives, and in plaque-purified substrains. this heterogeneity was due to variations in the copy number of a 14-base-pair tandem repeat comprising the base sequence 5'-gctcctcctccctc-3', which also exists, with some differenc ... | 1986 | 3005657 |
| occurrence of antibodies to the etiologic agents of infectious bovine rhinotracheitis, parainfluenza 3, leptospirosis, and brucellosis in white-tailed deer in minnesota. | a serologic survey was conducted on 628 white-tailed deer (odocoileus virginianus) in 1976 and 1979-1980. tests for antibodies to the etiologic agents of infectious bovine rhinotrancheitis (ibr), parainfluenza 3 (pi3), leptospirosis, and brucellosis produced positive results of 15%, 20%, 3% and 0%, respectively. adult deer had significantly higher prevalence of antibodies to ibr virus and pi3 virus than fawns. these data provide a basis for monitoring these disease agents in minnesota's white-ta ... | 1986 | 3005666 |
| hemagglutinating activity associated with bovine herpesvirus type 1. | using c57bl/hpb mouse erythrocytes, hemagglutination has been observed with the los angeles and colorado-1 strains of bovine herpesvirus type 1 and with 12 other canadian field isolates as well. the specificity of the hemagglutination observed with the viral strains has been confirmed by a hemagglutination-inhibition assay. | 1985 | 3006322 |
| study of immunity induced by the inactivated oil-adjuvant vaccine after natural infection with the virus of infectious bovine rhinotracheitis (ibr) in cattle. | | 1985 | 3006406 |
| bovine herpesvirus-1 and pasteurella haemolytica aerobiology in experimentally infected calves. | eight calves (2 calves in each of 4 groups) were exposed to an aerosol of bovine herpesvirus-1 (bhv-1) and 4 days later to an aerosol of pasteurella haemolytica. samples of tracheal and exhaled air were taken simultaneously beginning 1 day before viral exposure and once a day up to 3 to 4 days after the bacterial exposure. samples were also taken during the period of aerosol exposure. only 0.04% to 0.42% of p haemolytica-carrying droplets of the bacterial aerosol passed beyond the cranial part o ... | 1986 | 3006556 |
| intramuscular and intravaginal vaccination of pregnant cows with thymidine kinase-negative, temperature-resistant infectious bovine rhinotracheitis virus (bovine herpes virus 1). | to test the safety and efficacy of a thymidine kinase-negative (tk-), temperature-resistant (tr) mutant of bovine herpes virus-1 (bhv-1) in pregnant cows, seronegative cows, 2-5 months pregnant, were vaccinated intramuscularly (i.m.) or intravaginally (i.vag.) with this candidate vaccine virus. i.m. vaccinated cows did not shed virus i.vag. or intranasally (i.n.), but i.vag. vaccinated cows replicated virus i.vag. for 8-9 days postvaccination (p.v.) some of the cows were challenge exposed i.n. a ... | 1986 | 3008461 |
| detection of bovine herpesvirus-specific nucleic acids by in situ hybridization with biotinylated dna probes. | biotin-labeled dna probes for bovine herpesvirus type 1 (bhv-1) were used to detect viral nucleic acids in infected cell cultures and clinical specimens by in situ hybridization. hybridization signal was detected 2 hours after inoculation in the cytoplasm of infected cells, presumably representing input virus. hybridization was first detected in the nucleus at 4 hours after inoculation; by 10 hours after inoculation, hybridization signal was detected in both nucleus and cytoplasm of almost 50% o ... | 1986 | 3008605 |
| effect of recombinant dna-derived bovine and human interferons on replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses. | antiviral effects of recombinant dna-derived bovine (bo) and human (hu) interferons (ifn) on the replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses were studied. bovine monolayer cultures were treated with recombinant dna-produced bo ifn-alpha 1, bo ifn-beta 2, hu ifn-alpha a, or hu ifn-alpha a/d and then challenge exposed with bovine herpesvirus-1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, or vesicular stomatitis virus. treatment with ... | 1986 | 3008606 |
| polypeptide specificity of the antibody response after primary and recurrent infection with bovine herpesvirus 1. | the polypeptide specificities and defense mechanisms of the humoral immune response to bovine herpesvirus 1 were analyzed. sequential serum samples taken from cows which were experimentally infected with bovine herpesvirus 1 were tested for their reactivity with individual bovine herpesvirus 1 polypeptides by immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assay. all bovine immune sera reacted with each of the three major bovine herpesvirus 1 glycoproteins, gvp 6/11a/16, gvp ... | 1986 | 3009533 |
| logical description of bovine herpesvirus type 1 latent infection. | description of the interactions between bovine herpesvirus type 1 (bhv-1) and cattle was performed by the method known as kinetic logic. this logical formalization uses variables with two possible values, 1 and 0, which tell whether an element is present or not at a significant level. to each variable is associated a function which tells if the element is being produced at a significant rate. the temporal relation between a variable and its associated function is given by specific time delays. t ... | 1986 | 3009689 |
| [comparison of the immunizing effectiveness of subunit vaccine and inactivated viral oil vaccine against infectious rhinotracheitis of cattle]. | trials were conducted on rabbits and cattle to compare the immunizing effectiveness of the subunit vaccine against infectious bovine rhinotracheitis (ibr), representing antigens separated by the solubilization of the ibr virus-infected cells by means of triton x-100 with oil adjuvant, with the inactivated oil ibr vaccine. the rabbits inoculated and re-vaccinated with both vaccines in an interval of three weeks produced neutralizing antibodies in medium titres, the values of these antibodies were ... | 1986 | 3010533 |
| development of specific antibodies mediating antibody-dependent cell-mediated cytotoxicity following experimental infection of cattle with bovine herpesvirus 1 and subsequent viral reactivation. | evolution of sensitizing antibodies involved in antibody-dependent cell-mediated cytotoxicity (adcc), antibody-complement cell lysis and complement-facilitated adcc was followed in bulls after primary infection by a wild strain of infectious bovine rhinotracheitis virus (bovine herpesvirus 1; bhv-1) and after experimental reactivation of the virus. these antibodies were detected between the 4th and the 7th day after primary infection, reached a maximum level after 2 weeks and rose slightly after ... | 1986 | 3010549 |
| neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus. | infectious bovine rhinotracheitis virus (ibrv) has been shown in this report to have thirty-three polypeptides. ten of the eleven polypeptides which can be labeled with (3h)-glucosamine are located on the surface of the virus since they can be surface labeled with sodium boro(3h)hydride. in order to define the immunologically important viral proteins, monoclonal antibodies were prepared against the virus and selected for their ability to neutralize infectivity. four such hybridoma lines were obt ... | 1986 | 3010905 |
| nitrogen metabolism of calves inoculated with bovine adenovirus-3 or with infectious bovine rhinotracheitis virus. | beef calves were inoculated with bovine adenovirus-3 or infectious bovine rhinotracheitis virus. after inoculation, plasma fibrinogen increased, serum phosphorus decreased, and nitrogen and phosphorus digestibility decreased compared with preinoculation values. urinary n excretion increased when calves developed rectal temperatures greater than 39.7 c. results indicated that clinical infection of calves with infectious bovine rhinotracheitis virus increases urinary n excretion and reduces n and ... | 1986 | 3013053 |
| influence of induced disease states on the disposition kinetics of imidocarb in goats. | the influence of fever, induced by different agents, on the disposition kinetics of imidocarb was determined in goats. escherichia coli endotoxin (0.2 microgram/kg), trypanosoma evansi (10(7) in 1 ml sterile glucose citrate), and infectious bovine rhinotracheitis virus (10(6.5)tcid50) were the agents administered to induce the febrile state. in control and febrile animals the two-compartment model was used to describe the disposition kinetics of the drug. fever caused significant changes to occu ... | 1986 | 3014166 |
| enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses. | an enzyme linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against infectious bovine rhinotracheitis (ibr), parainfluenza-3 (pi3), adenovirus type 3 (adeno 3) and bovine respiratory syncytial (brs) viruses. paired serum samples from calves vaccinated with live attenuated virus vaccines were tested. the elisa compared favorably with the virus neutralization test for detecting serologic responses to ibr, brs, and adeno 3 viruses or with the hemagglutination inhib ... | 1986 | 3014711 |
| identification of bovine herpesvirus-1 polypeptides involved in serum neutralization. | bovine herpesvirus (infectious bovine rhinotracheitis virus)-infected cell antigens were solubilized with nonidet p-40. the crude antigen extract was separated by reaction with bovine hyperimmune serum in line immunoelectrophoresis; individual immunoprecipitates were used to immunize rabbits. rabbit sera possessing serum neutralizing activity were analyzed by reaction with crude antigen extract in immunoprecipitation sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page) and western ... | 1986 | 3016973 |
| evidence for an immunocompromising effect of bovine pestivirus on bovid herpesvirus 1 vaccination. | five calves were given live intranasal vaccine against bovid herpesvirus 1 (bhv1) two days after intranasal inoculation of bovine pestivirus (bvdv). another 5 were vaccinated in the absence of bvdv. control unvaccinated groups were also maintained. all calves were challenged with virulent bhv1. the unvaccinated calves developed signs of infectious bovine rhinotracheitis (ibr) and both vaccinated groups showed a similar degree of clinical protection from ibr. those given bvdv before vaccination s ... | 1986 | 3016976 |
| induction of immunity against pneumonic pasteurellosis following experimental infection in calves. | immunity against pneumonic pasteurellosis was studied in calves after recovery from experimental respiratory disease with pasteurella haemolytica. nine calves were exposed to aerosols of parainfluenza-3 virus and pasteurella haemolytica a1 six days apart to produce respiratory disease. after recovery from the disease, these nine principal and four control calves were challenged with aerosols of bovine herpesvirus 1 and p. haemolytica a1 four days apart. with this viral-bacterial challenge, the n ... | 1986 | 3017526 |
| the association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in ontario feedlot calves. | a seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. a total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). titers t ... | 1986 | 3017529 |
| immunologic comparison of the proteins of pseudorabies (aujeszky's disease) virus and bovine herpesvirus-1. | the immunologic relationship between bovine herpesvirus-1 and pseudorabies virus was examined by 80% serum cross-neutralization test, enzyme-linked immunosorbent assays, and western immunoblotting procedures. immunogenic cross reactivity between the 2 viruses was observed with both the serum-neutralization test and the enzyme-linked immunosorbent assay. a probing of viral western immunoblots with rabbit hyperimmune antiserum showed that there were a number of viral-specific cross-reactive protei ... | 1986 | 3019188 |
| investigation of possible vaccine-induced epizootics of infectious bovine rhinotracheitis, using restriction endonuclease analysis of viral dna. | viral dna was extracted from each of 14 modified-live (ml) bovine herpesvirus 1 vaccines, representing all of the ml infectious bovine rhinotracheitis virus (ibrv) vaccines licensed by the us department of agriculture for use in cattle. restriction endonucleases pst i and bgl ii were used to establish restriction enzyme patterns for the vaccinal viruses. viral dna from isolates obtained from 6 field samples of ibrv (1 from colorado, 1 from west virginia, 3 from wisconsin, 1 from south dakota) we ... | 1986 | 3019192 |
| [ibr/ipv serodiagnosis with elisa methods in blood, single milk and bulk milk samples; control measures for maintaining normal cattle herds; eradication measures with reference to vaccination]. | | 1986 | 3019627 |
| detection of impaired t cell-mediated immune responses to herpesvirus (bhv-1) in cattle. | interleukin 2 (il-2) functions in the regulation of cell-mediated immune responses both in vivo and in vitro. therefore, il-2 production has been studied in patients with immunological disorders to determine the level of the immune defect. however, the cause(s) of low responses to selected antigens by cells from clinically normal individuals has not been examined. the ability of cells from clinically normal individual cattle to produce and respond to il-2 was investigated as a measure of specifi ... | 1986 | 3020771 |
| map location of the gene for a 130,000-dalton glycoprotein of bovine herpesvirus 1. | a bovine herpesvirus 1 variant (mar6) containing a mutation in a viral glycoprotein with a molecular weight of 130,000 (g130) was isolated by selecting for resistance to a neutralizing monoclonal antibody (130-6) directed against g130. mar6 was completely resistant to neutralization by monoclonal antibody 130-6 in the presence and absence of complement, but was neutralized by polyvalent immune sera. the mar6 mutant synthesized and processed g130, but produced plaques which failed to react with m ... | 1986 | 3021976 |
| [prevalence of bovine herpesvirus 4 in the swiss cattle population and possible serologic cross reaction with bovine herpesvirus 1 (ibr/ipv virus)]. | | 1986 | 3022374 |
| identification of the thymidine kinase gene of infectious bovine rhinotracheitis virus and its function in escherichia coli hosts. | in an attempt to understand the gene expression of the infectious bovine rhinotracheitis virus (ibrv), the viral thymidine kinase gene (tk), a well regulated viral gene has been chosen for this study. a cosmid library of ibrv has been constructed in escherichia coli hb101 by cloning partially sau3a-digested dna fragments into a cosmid vector, pjb8. recombinant cosmids were further analyzed by restriction digestions and by southern blot hybridization. results showed that this cosmid library compr ... | 1986 | 3023190 |
| detection of bovine herpesvirus type 1 rna in trigeminal ganglia of latently infected rabbits by in situ hybridization. | at times after conjunctival inoculation with bovine herpesvirus type 1 (bhv-1), representing the acute and latent phases of infection, rabbit trigeminal ganglia were examined for the presence of bhv-1 nucleic acids by in situ hybridization using a 3h-labelled bhv-1 dna probe. during the acute phase of virus infection, both bhv-1 dna and rna were detected in ganglionic neurons and occasionally in adjacent satellite cells. however, during the latent phase of infection only viral rna was detectable ... | 1986 | 3023537 |
| in vitro induction of cytotoxic lymphocytes from infectious bovine rhinotracheitis virus hyperimmune cattle. | cytotoxic lymphocytes against infectious bovine rhinotracheitis virus (ibrv)-infected cells were induced by in vitro stimulation with ibrv antigen of peripheral blood leukocytes obtained from hyperimmune cattle. after one in vitro stimulation with ibrv, cytotoxicity was detected against ibrv-infected autologous target cells, but not against heterologous ibrv-infected or k562 target cells. after 4 in vitro ibrv stimulations at weekly intervals (in the presence of autologous feeder cells followed ... | 1986 | 3024535 |
| reduction in in vivo testing at the national veterinary services laboratories of the united states department of agriculture. | efforts are being made at the national veterinary services laboratories to reduce in vivo testing of usda licensed veterinary vaccines. a hemagglutination test for determining potency of killed parvovirus vaccine is currently being used for canine and swine adjuvanted and nonadjuvanted products; a serum neutralization inhibition test (snit) is being developed for potency testing of killed adjuvanted infectious bovine rhinotracheitis (ibr), bovine virus diarrhea (bvd) and parainfluenza (pi3) vacc ... | 1986 | 3025035 |
| cell-mediated cytotoxicity of peripheral blood mononuclear cells stimulated in vitro for infectious bovine rhinotracheitis virus-infected cells. | peripheral blood mononuclear cells (pbmc) from calves infected with and hyperimmunized to infectious bovine rhinotracheitis virus (ibrv) were stimulated in vitro with viral antigens to evaluate their cytotoxicity for a variety of cells. the 51-cr release assay was used to measure cytotoxicity. cytotoxicity was not present in fresh nonstimulated cells, but was detected in cultured, ibrv-stimulated cells at day 3, was maximal at day 7, and declined thereafter. pbmc stimulated in vitro with ibrv ex ... | 1986 | 3026081 |
| the genome of bovine herpesvirus 1 (bhv-1) strains exhibiting a neuropathogenic potential compared to known bhv-1 strains by restriction site mapping and cross-hybridization. | bovine herpesvirus 1 (bhv-1) strains can be differentiated by their dna and polypeptide patterns, and by antigenic properties as demonstrated by monoclonal antibodies. we classified the bhv-1 strains according to these data as bhv-1.1, bhv-1.2 (a/b) and bhv-1.3 (a/b). bhv-1.1 and bhv-1.2 correspond to the well known 'common' bhv-1 strains, whereas bhv-1.3 has only recently been recognized and exhibits a neuropathogenic potential. in the present paper we describe the structural genome characteris ... | 1986 | 3026111 |
| restriction endonuclease patterns of bovine herpesvirus type 1 isolated from bovine mammary glands. | restriction endonuclease analysis was used, in conjunction with viral neutralization and growth-curve experiments, to compare a bovine herpesvirus type 1 (bhv-1) isolate, originally obtained from bovine mammary gland lesions, with a standard bhv-1 strain, infectious bovine rhinotracheitis virus. although differences were not detected by viral neutralization or growth-curve experiments, restriction fragment patterns generated by bam hi, eco ri, hind iii, and hpa i, revealed definite differences b ... | 1986 | 3026212 |
| comparative efficacy of three 2'-fluoropyrimidine nucleosides and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (bw b759u) against pseudorabies and equine rhinopneumonitis virus infection in vitro and in laboratory animals. | the three 2'-fluoropyrimidine nucleosides 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac), 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), showed high activity in rk13 monolayers against equine rhinopneumonitis virus, (ehv-1, ic50 range 0.02-0.18 microm), aujeszky's disease virus (shv-1, pseudorabies, ic50 range 0.25-7 microm) and infectious bovine rhinotracheitis virus (1br, bhv-1, ic50 ... | 1987 | 3026244 |
| intracellular localization and transport of three different bovine herpesvirus type 1 glycoproteins involved in neutralization. | monoclonal antibodies against 3 different glycoproteins of bovine herpesvirus type 1 (bhv-1) involved in virus neutralization were used in indirect immunofluorescence (iif) tests to characterize the appearance and transport to the plasma membrane of virus antigens in the infected cells. antibodies against gp 117 and gp 71 glycoproteins first showed pronounced ring-like nuclear fluorescence at 4 hours post-infection (pi), followed by staining of the perinuclear region, presumably the golgi appara ... | 1987 | 3026288 |
| an evaluation of five serological tests for the detection of antibody to bovine herpesvirus 1 in vaccinated and experimentally infected cattle. | more than 300 bovine sera from a previously reported vaccination and challenge trial were tested for antibodies to bovine herpesvirus 1 (bhv1) by five serological assays: enzyme-linked immunosorbent assay (elisa) for igm and igg, passive haemagglutination (pha), and two methods of virus neutralisation (vn). in a statistical comparison of elisa (igg), pha and vn results, the assays showed highly significant correlations (p less than 0.01). the sensitivities of elisa and 24-hour neutralisation tes ... | 1986 | 3027800 |
| [bvd (bovine virus diarrhea) and ibr (infectious bovine rhinotracheitis): serologic studies on 35 farms]. | 1099 female cattle from 35 farms in the provinces of north brabant, gelderland and overijssel were examined for the presence of antibodies against bovine virus diarrhoea (bvd) and infectious bovine rhinotracheitis (ibr) by serum neutralisation tests. one of the farms examined was seronegative for bvd and 11 farms were seronegative for ibr. on the seropositive farms about 50% of cattle carrying their first calf had not experienced infection i.e. were seronegative (bvd 51.2%, ibr 48.3%). after cal ... | 1986 | 3027917 |
| patterns of bovine t cell-mediated immune responses to bovine herpesvirus 1. | lymphocyte proliferation was used to evaluate t cell-mediated immune responses to different isolates of bovine herpesvirus 1 (bhv-1). groups of high, moderate, and low responses were observed when lymphocytes from three breeds of dairy cattle were stimulated with each of the bhv-1 isolates. proliferation of cells in the low responding group could be augmented by exogenous il-2. the mechanism of unresponsiveness by cells from one individual whose response was not altered by il-2 supplementation w ... | 1986 | 3029942 |
| natural cell-mediated cytotoxicity to cells infected with infectious bovine rhinotracheitis virus. | cell-mediated cytotoxicity against viral-infected cells was demonstrated in a 6-hr 51cr release assay. peripheral blood mononuclear leukocytes from both infectious bovine rhinotracheitis virus (ibrv)-infected and noninfected cattle exhibited preferential lysis against ibrv-infected primary bovine embryonic kidney (bek) cells compared to cells infected with pseudorabies virus and noninfected bek cells. addition of specific antibody to the assay did not enhance cytotoxicity. the effector cell was ... | 1987 | 3029944 |
| inheritance and interaction of immune traits in beef calves. | three sets of blood samples were obtained from beef calves of two experimental populations and assayed for various immunological measurements. the first set of samples was taken between 24 and 48 h after birth and quantified for igg1 concentration. a second set was taken immediately prior to vaccination for infectious bovine rhinotracheitis virus (ibrv; at an average age of 164 d) and a third set taken 60 d post-vaccination. these later samples were quantified for antibodies specific to ibrv. le ... | 1987 | 3030992 |
| hemagglutination inhibition and virus neutralizing response of rabbits inoculated with bovine herpesvirus 1 subunit vaccine. | the immunogenicity of bovine herpesvirus type 1 (bhv-1) hemagglutinin has been investigated. both live and nonionic detergent solubilized vaccines were prepared and 5000 hemagglutinating units (hau) were injected subcutaneously into rabbits. both types of vaccine induced a good antibody response but live virus was four times more efficient in inducing hemagglutination inhibiting and neutralizing antibodies than either triton x-100- or octylglucoside-solubilized subunit vaccine. blotting analysis ... | 1987 | 3031871 |
| highly sensitive antigen detection procedures for the diagnosis of infectious bovine rhinotracheitis: amplified elisa and reverse passive haemagglutination. | the sensitivity of an enzyme-linked immunosorbent assay (elisa) for the detection of bovid herpesvirus 1 antigen was increased by up to 50-fold using the biotin-avidin interaction to amplify the reaction, when compared with a simple sandwich elisa. an alternative immunoassay, reverse passive haemagglutination (rpha), had a similar sensitivity to the amplified elisa, and was technically simpler to perform. both the amplified elisa and the rpha could detect viral antigen in the nasal secretions of ... | 1987 | 3031873 |
| reactivation in calves of latent infection by bovid herpesvirus-4. | nine calves, six of which had been infected with strain 85/bh 16tv and three with strain 85/bh 232tn of bovid herpesvirus-4 (bhv-4), were treated with dexamethasone (dms) three months after infection. dms administration did not induce any clinical signs of disease, but bhv-4 was isolated from the nasal swabbings of all calves for a maximum of 8 days after the start of dms treatment. the virus was also isolated from the nerve tissues, nasal mucosa, lymph nodes, lung and spleen of 4 calves that we ... | 1987 | 3033450 |