| eggshell penetration of hen's eggs by salmonella enterica serovar enteritidis upon various storage conditions. | 1. the survival and penetration of salmonella enterica serovar enteritidis (se) inoculated on the eggshell was examined upon storage for up to 20 d at real-life conditions (15 to 25 degrees c and 45 to 75% relative humidity (rh)). 2. penetration was assessed by emptying the egg contents and filling the eggs with a selective medium that allowed visualising salmonella growth on the inside of the shell and membrane complex. 3. the study of survival on the eggshells was based on viable counts and sh ... | 2006 | 17050098 |
| misidentification of a mucoid strain of salmonella enterica serotype choleraesuis as hafnia alvei by the vitek gni+ card system. | nontyphoidal salmonellae are among the most common causes of bacterial gastroenteritis worldwide. they are also notable causes of extraintestinal infections, including bacteremia and vascular infections. salmonella enterica serotype choleraesuis is typically associated with invasive infections. we report a patient who had an infected intra-abdominal aortic aneurysm due to an unusually mucoid strain of salmonella enterica serotype choleraesuis. the isolate was erroneously identified as hafnia alv ... | 2006 | 17050821 |
| helicobacter pylori flhb function: the flhb c-terminal homologue hp1575 acts as a "spare part" to permit flagellar export when the hp0770 flhbcc domain is deleted. | in helicobacter pylori 26695, a gene annotated hp1575 encodes a putative protein of unknown function which shows significant similarity to part of the c-terminal domain of the flagellar export protein flhb. in salmonella enterica, this part (flhb(cc)) is proteolytically cleaved from the full-length flhb, a processing event that is required for flagellar protein export and, thus, motility. the role of flhb (hp0770) and its c-terminal homologue hp1575 was studied in h. pylori using a range of nonp ... | 2006 | 17050924 |
| class 1 and class 2 integrons in non-prevalent serovars of salmonella enterica: structure and association with transposons and plasmids. | to characterize class 1 and class 2 integrons which were simultaneously detected in non-typhoid salmonella enterica strains of non-prevalent serovars, and to investigate their possible association with transposons and/or plasmids. | 2006 | 17056609 |
| rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr. | buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ... | 2007 | 17056684 |
| sequence analysis of the rfb loci, encoding proteins involved in the biosynthesis of the salmonella enterica o17 and o18 antigens: serogroup-specific identification by pcr. | we report sequencing of the o antigen encoded by the rfb gene cluster of salmonella enterica serotype jangwani (o17) and salmonella serotype cerro (o18). we developed serogroup o17- and o18-specific pcr assays based on rfb gene targets and found them to be sensitive and specific for rapid identification of salmonella serogroups o17 and o18. | 2006 | 17056694 |
| engineering of deinococcus radiodurans r1 for bioprecipitation of uranium from dilute nuclear waste. | genetic engineering of radiation-resistant organisms to recover radionuclides/heavy metals from radioactive wastes is an attractive proposition. we have constructed a deinococcus radiodurans strain harboring phon, a gene encoding a nonspecific acid phosphatase, obtained from a local isolate of salmonella enterica serovar typhi. the recombinant strain expressed an approximately 27-kda active phon protein and efficiently precipitated over 90% of the uranium from a 0.8 mm uranyl nitrate solution in ... | 2006 | 17056698 |
| virulent salmonella enterica serovar typhimurium evades adaptive immunity by preventing dendritic cells from activating t cells. | dendritic cells (dcs) constitute the link between innate and adaptive immunity by directly recognizing pathogen-associated molecular patterns (pamps) in bacteria and by presenting bacterial antigens to t cells. recognition of pamps renders dcs as professional antigen-presenting cells able to prime naïve t cells and initiate adaptive immunity against bacteria. therefore, interfering with dc function would promote bacterial survival and dissemination. understanding the molecular mechanisms that ha ... | 2006 | 17057096 |
| dual mode of action of bac7, a proline-rich antibacterial peptide. | proline-rich peptides are a unique group of antimicrobial peptides that exert their activity selectively against gram-negative bacteria through an apparently non-membranolytic mode of action that is not yet well understood. we have investigated the mechanism underlying the antibacterial activity of the proline-rich cathelicidin bac7 against salmonella enterica and escherichia coli. the killing and membrane permeabilization kinetics as well as the cellular localization were assessed for the fully ... | 2006 | 17059867 |
| the yfgl lipoprotein is essential for type iii secretion system expression and virulence of salmonella enterica serovar enteritidis. | salmonella enterica, like many gram-negative pathogens, uses type three secretion systems (ttss) to infect its hosts. the three ttss of salmonella, namely, ttss-1, ttss-2, and flagella, play a major role in the virulence of this bacterium, allowing it to cross the intestinal barrier and to disseminate systemically. previous data from our laboratory have demonstrated the involvement of the chromosomal region harboring the yfgl, enga, and yfgj open reading frames in s. enterica serovar enteritidis ... | 2007 | 17060472 |
| detection and characterization of variant salmonella genomic island 1s from salmonella derby isolates. | the purpose of this study is to investigate the distribution and structure of salmonella genomic island 1 (sgi1) among salmonella enterica serovar derby isolates from swine and their rearing environment. three variants of sgi1s, specifically sgi1-a, c, and i, were identified by pcr mapping. the results of macro-restriction analysis and dna sequencing of sgi1 flanking regions revealed that there are at least two genomic lineages of derby strains bearing sgi1s. | 2006 | 17060705 |
| cgat: a comparative genome analysis tool for visualizing alignments in the analysis of complex evolutionary changes between closely related genomes. | the recent accumulation of closely related genomic sequences provides a valuable resource for the elucidation of the evolutionary histories of various organisms. however, although numerous alignment calculation and visualization tools have been developed to date, the analysis of complex genomic changes, such as large insertions, deletions, inversions, translocations and duplications, still presents certain difficulties. | 2006 | 17062155 |
| mismatch induced speciation in salmonella: model and data. | in bacteria, dna sequence mismatches act as a barrier to recombination between distantly related organisms and can potentially promote the cohesion of species. we have performed computer simulations which show that the homology dependence of recombination can cause de novo speciation in a neutrally evolving population once a critical population size has been exceeded. our model can explain the patterns of divergence and genetic exchange observed in the genus salmonella, without invoking either n ... | 2006 | 17062419 |
| effect of fluoroquinolone exposure on the proteome of salmonella enterica serovar typhimurium. | the physiological response of salmonella enterica serovar typhimurium to fluoroquinolone antibiotics was investigated using proteomic methods. | 2006 | 17062612 |
| cefotaxime-ciprofloxacin combination therapy for nontyphoid salmonella bacteremia and paravertebral abscess after failure of monotherapy. | therapeutic failure of monotherapy with either a third-generation cephalosporin or a fluoroquinolone against nontyphoid salmonellae has been observed in clinical practice. combination therapy with both agents is recommended in the literature for treating life-threatening infections. however, we know of no published case reports that indicate a therapeutic advantage of this combination therapy for nontyphoid salmonellae infections. we describe a 60-year-old man who had breakthrough bacteremia wit ... | 2006 | 17064216 |
| comparison of serological test kits for diagnosis of typhoid fever in the philippines. | we evaluated four recent antibody-detection kits for typhoid fever by using 177 febrile patients from our hospital, in 75 of whom salmonella enterica serotype typhi grew. tubex performed best, achieving 94.7% sensitivity and 80.4% specificity. typhidot, sd bioline typhoid, and mega salmonella were less specific and, in most cases, less sensitive. | 2007 | 17065261 |
| bacteremia caused by salmonella enterica serotype choleraesuis in taiwan. | since 1995, there has been a steady increase in the number of reported cases of salmonella enterica serotype choleraesuis (s. choleraesuis) sepsis in taiwan. representative taiwanese survey data from 1996 to 2004 revealed that these adult patients with s. choleraesuis bacteremia presented with primary bacteremia (57%, especially immunocompromised hosts), mycotic aneurysm (16%), and fever (86%) predominantly. s. choleraesuis septicemia demonstrated a higher invasion index (with secondary involved ... | 2006 | 17066196 |
| salmonella prevalence and total microbial and spore populations in spices imported to japan. | a total of 259 samples of 40 types of spices were tested for salmonella prevalence and total microbial and spore populations. salmonella enterica serotypes weltevreden and senftenberg were isolated from a black- and red-pepper sample, respectively. because salmonella was not detected by the most-probable-number method, it indicated that at least one cell of the microorganism was present in 25 g of sample. the mean aerobic bacterial count was greater than 5.39 log cfu/g in turmeric, garam masala, ... | 2006 | 17066938 |
| comparison of the in vitro pathogenicity of two salmonella typhimurium phage types. | the in vitro pathogenicity of salmonella enterica serovar typhimurium phage type (pt) 90 and pt 506 (also known as dt 104) isolates from human and porcine origin was studied in adhesion and invasion assays to the human cell line caco-2 and the porcine cell line ipi-2. interleukin-8 (il-8) production by these two cell lines in response to stimulation by the two salmonella phage types was also measured. generally, salmonella typhimurium pt 506 and pt 90 adhered to and invaded caco-2 cells and ipi- ... | 2007 | 17067673 |
| biofilm-associated proteins. | although exopolysaccharides are important and often essential compounds of the biofilm matrix, recent evidences suggest that a group of surface proteins plays a leading role during the development of the microbial communities. the first member of this group of proteins was described in a staphylococcus aureus bovine mastitis isolate and was named bap, for biofilm-associated protein. later on, other surface proteins homologous to bap and involved in biofilm development have been described in many ... | 2006 | 17067927 |
| characterization of class 1 integron resistance gene cassettes in salmonella enterica serovars oslo and bareily from imported seafood. | | 2006 | 17068008 |
| isolation of salmonella enterica and shiga-toxigenic escherichia coli o157 from feces of animals in public contact areas of united states zoological parks. | the fecal prevalence of subclinical salmonella enterica and shiga-toxigenic escherichia coli o157 among animals in human-animal contact exhibits at institutions in the united states accredited by the association of zoos and aquariums was estimated to assess public health risk. the prevalence was less than 0.6% for both zoonotic pathogens among 997 animals sampled at 36 exhibits. | 2007 | 17071798 |
| molecular characterization of ciprofloxacin-resistant salmonella enterica serovar typhi and paratyphi a causing enteric fever in india. | to define the genetic characteristics and resistance mechanisms of clinical isolates of salmonella enterica serovar typhi (s. typhi) and s. enterica serovar paratyphi a (s. paratyphi a) exhibiting high-level fluoroquinolones resistance. | 2006 | 17071955 |
| differential activation and function of rho gtpases during salmonella-host cell interactions. | salmonella enterica, the cause of food poisoning and typhoid fever, has evolved sophisticated mechanisms to modulate rho family guanosine triphosphatases (gtpases) to mediate specific cellular responses such as actin remodeling, macropinocytosis, and nuclear responses. these responses are largely the result of the activity of a set of bacterial proteins (sope, sope2, and sopb) that, upon delivery into host cells via a type iii secretion system, activate specific rho family gtpases either directl ... | 2006 | 17074883 |
| contribution of the sira regulon to biofilm formation in salmonella enterica serovar typhimurium. | orthologues of the salmonella enterica serovar typhimurium (s. typhimurium) bara/sira two-component system are important for biofilm formation and virulence in many gamma-proteobacteria. in s. typhimurium, sira activates the csrb and csrc carbon storage regulatory rnas and the virulence gene regulators hila and hilc. the regulatory rnas antagonize the activity of the csra protein, allowing translation of those same virulence genes, and inhibiting the translation of flagellar genes. in this repor ... | 2006 | 17074910 |
| [gastroenteritis caused by salmonella from pet snakes]. | a salmonella subspecies associated with reptiles (salmonella enterica subspecies diarizonae) was isolated from the stool of a 19-year-old man with gastroenteritis. the same species was isolated from stool and urine samples taken from terraria found in the home of the patient's parents where snakes were kept. a high percentage of reptiles in the wild and in captivity are asymptomatic carriers of salmonella species that can be transmitted to humans who come in contact with these animals. unlike in ... | 2006 | 17076364 |
| salmonella produces an o-antigen capsule regulated by agfd and important for environmental persistence. | in this study, we show that salmonella produces an o-antigen capsule coregulated with the fimbria- and cellulose-associated extracellular matrix. structural analysis of purified salmonella extracellular polysaccharides yielded predominantly a repeating oligosaccharide unit similar to that of salmonella enterica serovar enteritidis lipopolysaccharide o antigen with some modifications. putative carbohydrate transport and regulatory operons important for capsule assembly and translocation, designat ... | 2006 | 17079680 |
| a novel multiplex pcr assay for the detection of salmonella enterica serovar enteritidis in human faeces. | to develop a multiplex pcr assay for the detection of salmonella enterica serovar enteritidis in human faeces. | 2006 | 17083715 |
| synthesis of n-acyl homoserine lactone analogues reveals strong activators of sdia, the salmonella enterica serovar typhimurium luxr homologue. | n-acyl homoserine lactones (ahls) are molecules that are synthesized and detected by many gram-negative bacteria to monitor the population density, a phenomenon known as quorum sensing. salmonella enterica serovar typhimurium is an exceptional species since it does not synthesize its own ahls, while it does encode a luxr homologue, sdia, which enables this bacterium to detect ahls that are produced by other species. to obtain more information about the specificity of the ligand binding by sdia, ... | 2007 | 17085703 |
| sgi1-k, a variant of the sgi1 genomic island carrying a mercury resistance region, in salmonella enterica serovar kentucky. | a multiple-antibiotic-resistant salmonella enterica serovar kentucky strain was found to contain sgi1-k, a variant form of the salmonella genomic island 1 (sgi1) with an in4-type class 1 integron that contains only one cassette array, aacca5-aada7, and an adjacent mercury resistance module. part of the 3'-conserved segment (3'-cs) of the integron, together with the inverted short segment from the right-hand end of the integron transposition module normally found between the 3'-cs and is6100 in i ... | 2007 | 17088481 |
| increase in nalidixic acid resistance among non-typhi salmonella enterica isolates in the united states from 1996 to 2003. | fluoroquinolones commonly are used to treat adult salmonella infections. fluoroquinolone treatment has failed for persons infected with nalidixic acid-resistant salmonella. from 1996 to 2003, state public health laboratories forwarded 12,252 non-typhi salmonella enterica isolates to the centers for disease control and prevention for antimicrobial susceptibility testing; 203 (1.6%) of the isolates were nalidixic acid resistant, and 14 (7%) of those were ciprofloxacin resistant. resistance to nali ... | 2007 | 17088493 |
| antibacterial activity of coffee extracts and selected coffee chemical compounds against enterobacteria. | the in vitro antimicrobial activity of commercial coffee extracts and chemical compounds was investigated on nine strains of enterobacteria. the antimicrobial activity investigated by the disc diffusion method was observed in both the extracts and tested chemical compounds. even though ph, color, and the contents of trigonelline, caffeine, and chlorogenic acids differed significantly among the coffee extracts, no significant differences were observed in their antimicrobial activity. caffeic acid ... | 2006 | 17090115 |
| a bimodal pattern of relatedness between the salmonella paratyphi a and typhi genomes: convergence or divergence by homologous recombination? | all salmonella can cause disease but severe systemic infections are primarily caused by a few lineages. paratyphi a and typhi are the deadliest human restricted serovars, responsible for approximately 600,000 deaths per annum. we developed a bayesian changepoint model that uses variation in the degree of nucleotide divergence along two genomes to detect homologous recombination between these strains, and with other lineages of salmonella enterica. paratyphi a and typhi showed an atypical and sur ... | 2007 | 17090663 |
| isolation of salmonella enteritidis phage type 21b from a eurasian eagle-owl (bubo bubo). | a case of fatal salmonellosis in a eurasian eagle-owl (bubo bubo) from bursa province (northwestern turkey) is described. the organs of the bird were examined histopathologically and microbiologically. macroscopic and microscopic findings were consistent with a salmonella infection. salmonella enterica subspecies enterica serovar enteritidis (s. enteritidis) phage type (pt) 21b was isolated from the liver and spleen in pure culture and from the intestine. the isolate was susceptible to amoxycill ... | 2006 | 17092905 |
| prevalence and serovars of salmonella in the feces of free-ranging white-tailed deer (odocoileus virginianus) in nebraska. | to determine the prevalence and serovars of salmonella in free-ranging deer, we cultured feces from white-tailed deer (odocoileus virginianus) harvested by hunters during a regular firearm season in southeastern nebraska (usa). we recovered salmonella from 5 (1%; 95% confidence interval: 0.37-2.20%) of 500 samples and identified four different salmonella enterica serovars [litchfield (1), dessau (1), infantis (2), and enteritidis (1)]. although the prevalence of salmonella in free-ranging deer a ... | 2006 | 17092906 |
| proteinase-activated receptors induce nonoxidative, antimicrobial peptides and increased antimicrobial activity in human mononuclear phagocytes. | as thrombin and sfllrnpndkyepf (sfllrn-14), a synthetic ligand, mainly of the proteinase-activated receptor-1 (par-1), induce in monocytes the synthesis and secretion of chemokines, the par pathway can be viewed as a mononuclear phagocyte-activating principle. classically, antimicrobial activity of mononuclear phagocytes is the measure for activation. here, we investigated whether thrombin or sfllrn-14 increases the antimicrobial activity of human monocytes and compared these effects to those of ... | 2007 | 17095611 |
| transmission of salmonella enterica serotype typhimurium in poultry with and without antimicrobial selective pressure. | to determine the effect of antimicrobial selective pressure on the transmission of antimicrobial resistant and sensitive strains of salmonella in poultry. | 2006 | 17105560 |
| comparison of salmonella enterica serovar distribution and antibiotic resistance patterns in wastewater at municipal water treatment plants in two california cities. | to determine salmonella enterica serovars and antibiotic resistance (abr) in the human waste stream. | 2006 | 17105561 |
| isolation of salmonella enterica subspecies enterica serovar paratyphi b dt+, or salmonella java, from indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the orf stm 3356. | salmonella enterica subspecies enterica serovar paratyphi b [o1,4,(5),12 : hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. the d-tartrate non-fermenting variant s. enterica subsp. enterica serovar paratyphi b dt- (s. paratyphi b) is the causative agent of paratyphoid fever, and the d-tartrate fermenting variant s. enterica subsp. enterica serovar paratyphi b dt+ (s. paratyphi b dt+; formerly called salmonella java) causes gastroenteriti ... | 2006 | 17108269 |
| identification of the salmonella phage epsilon 34 tailspike gene. | to understand the interaction between lipopolysaccharide (lps) and proteins in molecular detail, a molecular genetic approach has been employed, using phage as a model system. the phage epsilon(34) is a salmonella phage whose tailspike protein (tsp) uses the host lps as its initial host cell receptor. previous studies indicated that there was a similarity between the well-studied tail protein of salmonella phage p22 and the epsilon(34). this study reports the identification of the gene for the e ... | 2007 | 17113244 |
| proteus mirabilis clinical isolate harbouring a new variant of salmonella genomic island 1 containing the multiple antibiotic resistance region. | a clinical isolate of proteus mirabilis strain 18306, which displayed the multidrug resistance phenotype of salmonella genomic island 1 (sgi1), was examined for the presence of this island including its multiple antibiotic resistance genomic region. | 2007 | 17114173 |
| variant salmonella genomic island 1 antibiotic resistance gene clusters in salmonella enterica serovar derby isolates from humans in taiwan. | | 2007 | 17114174 |
| caenorhabditis elegans as a model to determine fitness of antibiotic-resistant salmonella enterica serovar typhimurium. | we used the ability of salmonella enterica serovar typhimurium to colonize the gut of caenorhabditis elegans to measure the fitness costs imposed by antibiotic resistance mutations. the fitness costs determined in the nematode were similar to those measured in mice, validating its use as a simple host model to evaluate bacterial fitness. | 2007 | 17116682 |
| colonic irritation. | | 2006 | 17120341 |
| infection and excretion of salmonella enteritidis in two different chicken lines with concurrent ascaridia galli infection. | studies on the impact of interaction of salmonella enterica serovar enteritidis and the parasitic nematode ascaridia galli with the avian host were undertaken with particular emphasis on infection and excretion of these pathogens in two different layer lines. a total of 148 salmonella-free 1-day-old chickens (73 hellevad and 75 lohmann brown) were randomly divided into five groups for each line. group 1 served as an uninoculated control group. groups 2 and 3 were infected with a. galli and s. en ... | 2006 | 17121738 |
| molecular differentiation of common promoters in salmonella class 1 integrons. | the integron is a mobile gene element which harbors antibiotic-resistance gene cassettes capable of site-specific integration. among the four known types of integrons, the class 1 integron has been associated with multidrug-resistance in pathogenic bacteria. these gene cassettes have been the focus of a series of studies. the gene cassettes share a common promoter, and their expression levels are affected not only by their proximity to the promoter, but also by the strength (weak, hybrid and str ... | 2007 | 17123653 |
| the contribution of cytolethal distending toxin to bacterial pathogenesis. | cytolethal distending toxin (cdt) is a bacterial toxin that initiates a eukaryotic cell cycle block at the g2 stage prior to mitosis. cdt is produced by a number of bacterial pathogens including: campylobacter species, escherichia coli, salmonella enterica serovar typhi, shigella dystenteriae, enterohepatic helicobacter species, actinobacillus actinomycetemcomitans (the cause of aggressive periodontitis), and haemophilus ducreyi (the cause of chancroid). the functional toxin is composed of three ... | 2006 | 17123907 |
| causes of community-acquired bacteremia and patterns of antimicrobial resistance in vientiane, laos. | there is no published information on the causes of bacteremia in the lao pdr (laos). between 2000 and 2004, 4512 blood culture pairs were taken from patients admitted to mahosot hospital, vientiane, laos, with suspected community-acquired bacteremia; 483 (10.7%) cultures grew a clinically significant community-acquired organism, most commonly salmonella enterica serovar typhi (50.9%), staphylococcus aureus (19.0%), and escherichia coli (12.4%). s. aureus bacteremia was common among infants (69.2 ... | 2006 | 17124000 |
| evolutionary history of salmonella typhi. | for microbial pathogens, phylogeographic differentiation seems to be relatively common. however, the neutral population structure of salmonella enterica serovar typhi reflects the continued existence of ubiquitous haplotypes over millennia. in contrast, clinical use of fluoroquinolones has yielded at least 15 independent gyra mutations within a decade and stimulated clonal expansion of haplotype h58 in asia and africa. yet, antibiotic-sensitive strains and haplotypes other than h58 still persist ... | 2006 | 17124322 |
| a hospital-based study of bloodstream infections in febrile patients in dhulikhel hospital kathmandu university teaching hospital, nepal. | the etiology of bloodstream infections in febrile patients remain poorly characterized in nepal. a retrospective study of febrile patients presenting to dhulikhel hospital kathmandu university teaching hospital from july 2002 to june 2004 was performed to evaluate the etiology of bloodstream infections and the drug sensitivity patterns of cultured organisms. the medical and laboratory records of all febrile patients with an axillary temperature > or = 38 degrees c who had a blood culture taken ( ... | 2006 | 17124998 |
| impact of plasmid stability on oral dna delivery by salmonella enterica serovar typhimurium. | live attenuated salmonellae may overcome limitations with conventional methods of dna immunisation. this study examined the impact of plasmid stability on oral dna delivery by the attenuated salmonella enterica serovar typhimurium vaccine strain brd509. a dna vaccine cassette comprising the c fragment of tetanus toxin under control of the cytomegalovirus (cmv) promoter was ligated into plasmid pcdna3, puc18, pbbr122, pacyc184, prsf1010/cat, pbr322 and pat153. in vitro and in vivo stability studi ... | 2007 | 17126961 |
| a strain of salmonella enterica serovar virchow isolated in turkey and carrying a ctx-m-3 extended-spectrum beta-lactamase. | a multiply resistant strain of salmonella enterica subsp. enterica serovar virchow was isolated in november 2002 from a catheterized patient admitted to the ssk training hospital in ankara, turkey. this isolate showed an antimicrobial susceptibility pattern compatible with the presence of a ctx-m-type esbl, namely resistance to cefotaxime, aztreonam and cefepime, and intermediate susceptibility to ceftazidime. on checking for the presence of the bla(tem), bla(shv), and bla(ctx-m )resistance gene ... | 2006 | 17129843 |
| activity-based substrate profiling for gcn5-related n-acetyltransferases: the use of chloroacetyl-coenzyme a to identify protein substrates. | the gcn5-related n-acetyltransferases (gnat) comprise one of the largest enzyme superfamilies, with over 10 000 known members represented in all kingdoms of life. chloroacetylcoenzymea was prepared and demonstrated to be a substrate for several gnat members. chloroacetylcoa (claccoa) is used by the hat1 histone acetyltransferase to correctly acetylate histone h4 in a mixture of histone proteins. chloroacetylation can be assessed by the subsequent reaction of the chloroacetylated product with thi ... | 2006 | 17131985 |
| salmonella and shigella in freshly squeezed orange juice, fresh oranges, and wiping cloths collected from public markets and street booths in guadalajara, mexico: incidence and comparison of analytical routes. | a survey of the presence of salmonella and shigella in freshly squeezed orange juice and related samples was conducted in guadalajara, mexico. one hundred samples of freshly squeezed orange juice were collected from 49 street booths and 51 small food service establishments. in addition, 75 fresh orange samples, each consisting of five orange units, and 75 wiping cloths were collected from the same establishments from which juice had been collected. salmonella was isolated from 14, 20, and 23% of ... | 2006 | 17133801 |
| effects of microbial inhibitors and modified atmosphere packaging on growth of listeria monocytogenes and salmonella enterica typhimurium and on quality attributes of injected pork chops and sliced cured ham. | this study was designed to determine the inhibitory effects of modified atmosphere packaging (map), both alone and in combination with potassium lactate and sodium diacetate (plsd), on selected pathogens common to pork products. effects of the treatments on product quality also were assessed. the hypothesis was that high-co2 map would increase the effectiveness of plsd for inhibition of pork pathogens. fresh chops from untreated pork loins and loins that were injected with plsd were inoculated w ... | 2006 | 17133810 |
| survival, elongation, and elevated tolerance of salmonella enterica serovar enteritidis at reduced water activity. | growing microorganisms on dry surfaces, which results in exposure to low water activity (a(w)), may change their normal morphology and physiological activity. in this study, the morphological changes and cell viability of salmonella enterica serovar enteritidis challenged to low a(w) were analyzed. the results indicated that exposure to reduced a(w) induced filamentation of the cells. the amount of filamentous cells at a(w) 0.94 was up to 90% of the total number of cells. surviving filamentous c ... | 2006 | 17133811 |
| heat tolerance of salmonella enterica serovars agona, enteritidis, and typhimurium in peanut butter. | recent large foodborne outbreaks caused by salmonella enterica serovars have been associated with consumption of foods with high fat content and reduced water activity, even though their ingredients usually undergo pasteurization. the present study was focused on the heat tolerance of salmonella enterica serovars agona, enteritidis, and typhimurium in peanut butter. the salmonella serovars in the peanut butter were resistant to heat, and even at a temperature as high as 90 degrees c only 3.2-log ... | 2006 | 17133812 |
| stage of primary infection with lymphocytic choriomeningitis virus determines predisposition or resistance of mice to secondary bacterial infections. | we investigated the effect of a primary non-lethal infection with lymphocytic choriomeningitis virus (lcmv) on the course and outcome of a secondary infection with the gram-negative salmonella enterica serovar typhimurium or the gram-positive listeria monocytogenes in mice. we found that at each stage of the viral infection the susceptibility of mice to bacterial super-infections changes dramatically and depends also on whether the secondary infection is a gram-positive or gram-negative one. the ... | 2007 | 17136407 |
| effect of glucose or glycerol as the sole carbon source on gene expression from the salmonella prpbcde promoter in escherichia coli. | we have developed an expression system (salmonella-based ppro system) containing the salmonella enterica prpbcde promoter (pprpb) and prpr encoding the positive transcriptional regulator of this promoter. in this study, the transcriptional efficiency of the ppro expression system was measured by placing the gene encoding the green fluorescent protein (gfp) under the control of pprpb and growing cells containing this construct in minimal medium supplemented with glucose or glycerol as a sole carb ... | 2006 | 17137300 |
| detection and characterization of salmonella associated with tropical seafood. | the prevalence of salmonella in seafood samples collected from the southwest coast of india was studied by conventional culture and by a dna based molecular technique, polymerase chain reaction (pcr). while conventional culture techniques detected salmonella in only 20 out of the 100 samples analyzed, direct enrichment lysate pcr detected 52 as positive for salmonella. a set of three different pcr primers viz., hns, inva and inve were used. it was observed that hns primer detected salmonella in ... | 2007 | 17141346 |
| environmental stress and antibiotic resistance in food-related pathogens. | this study investigated the possibility that sublethal food preservation stresses (high or low temperature and osmotic and ph stress) can lead to changes in the nature and scale of antibiotic resistance (abr) expressed by three food-related pathogens (escherichia coli, salmonella enterica serovar typhimurium, and staphylococcus aureus). the study found that some sublethal stresses significantly altered antibiotic resistance. incubation at sublethal high temperature (45 degrees c) decreased abr. ... | 2007 | 17142359 |
| novel quantitative biosystem for modeling physiological fluid shear stress on cells. | the response of microbes to changes in the mechanical force of fluid shear has important implications for pathogens, which experience wide fluctuations in fluid shear in vivo during infection. however, the majority of studies have not cultured microbes under physiological fluid shear conditions within a range commonly encountered by microbes during host-pathogen interactions. here we describe a convenient batch culture biosystem in which (i) the levels of fluid shear force can be varied within p ... | 2007 | 17142365 |
| randomized controlled comparison of ofloxacin, azithromycin, and an ofloxacin-azithromycin combination for treatment of multidrug-resistant and nalidixic acid-resistant typhoid fever. | isolates of salmonella enterica serovar typhi that are multidrug resistant (mdr, resistant to chloramphenicol, ampicillin, and trimethoprim-sulfamethoxazole) and have reduced susceptibility to fluoroquinolones (nalidixic acid resistant, na(r)) are common in asia. the optimum treatment for infections caused by such isolates is not established. this study compared different antimicrobial regimens for the treatment of mdr/na(r) typhoid fever. vietnamese children and adults with uncomplicated typhoi ... | 2007 | 17145784 |
| oral administration of a salmonella enterica-based vaccine expressing bacillus anthracis protective antigen confers protection against aerosolized b. anthracis. | bacillus anthracis is the causative agent of anthrax, a disease that affects wildlife, livestock, and humans. protection against anthrax is primarily afforded by immunity to the b. anthracis protective antigen (pa), particularly pa domains 4 and 1. to further the development of an orally delivered human vaccine for mass vaccination against anthrax, we produced salmonella enterica serovar typhimurium expressing full-length pa, pa domains 1 and 4, or pa domain 4 using codon-optimized pa dna fused ... | 2007 | 17145938 |
| involvement of a salmonella genomic island 1 gene in the rumen protozoan-mediated enhancement of invasion for multiple-antibiotic-resistant salmonella enterica serovar typhimurium. | multiple-antibiotic-resistant salmonella enterica serotype typhimurium is a food-borne pathogen that may be more virulent than related strains lacking the multiresistance phenotype. salmonella enterica serotype typhimurium phage type dt104 is the most prevalent of these multiresistant/hypervirulent strains. multiresistance in dt104 is conferred by an integron structure, designated salmonella genomic island 1 (sgi1), while we recently demonstrated dt104 hyperinvasion mediated by rumen protozoa (r ... | 2007 | 17145942 |
| salmonella, the host and disease: a brief review. | salmonella species cause substantial morbidity, mortality and burden of disease globally. infections with salmonella species cause multiple clinical syndromes. central to the pathophysiology of all human salmonelloses is the induction of a strong host innate immune/inflammatory response. whether this ultimately reflects an adaptive advantage to the host or pathogen is not clear. however, it is evident that both the host and pathogen have evolved mechanisms of triggering host responses that are d ... | 2007 | 17146467 |
| the evolution of a conjugative plasmid and its ability to increase bacterial fitness. | conjugative plasmids are extra-chromosomal dna elements that are capable of horizontal transmission and are found in many natural isolated bacteria. although plasmids may carry beneficial genes to their bacterial host, they may also cause a fitness cost. in this work, we studied the evolution of the r1 plasmid and we found that, in spite of the r1 plasmid conferring an initial cost to its host, after 420 generations the cost disappeared in all five independent evolution experiments. in fact, in ... | 2005 | 17148179 |
| comparison of multiple-locus variable-number tandem repeat analysis, pulsed-field gel electrophoresis, and phage typing for subtype analysis of salmonella enterica serotype enteritidis. | strain subtyping is an important tool for detection of outbreaks caused by salmonella enterica serotype enteritidis. current subtyping methods, however, yield less than optimal subtype discrimination. in this study, we describe the development and evaluation of a multiple-locus variable-number tandem repeat analysis (mlva) method for subtyping salmonella serotype enteritidis. the discrimination ability and epidemiological concordance of mlva were compared with those of pulsed-field gel electroph ... | 2007 | 17151203 |
| infectivity and persistence of an outbreak strain of salmonella enterica serotype typhimurium dt160 for house sparrows (passer domesticus) in new zealand. | to examine the infective dose, incubation period and disease progression of an isolate of salmonella enterica serotype typhimurium definitive type 160 (dt160) originating from a naturally-infected house sparrow (passer domesticus) during an outbreak of the disease in new zealand. | 2006 | 17151733 |
| effect of triclosan on salmonella typhimurium at different growth stages and in biofilms. | triclosan is a potent biocide that is included in a diverse range of products. this research was aimed to investigate the susceptibility of planktonic and biofilm-associated salmonella enterica serovar typhimurium to triclosan, and to identify potential mechanisms of adaptation. the effect of triclosan was studied on planktonic salmonella (log and stationary phases), on biofilm-associated cells, on bacteria derived from disrupted biofilms and on a biofilm-deficient mutant. an eight-log reduction ... | 2007 | 17156099 |
| high-resolution melting assay for the detection of gyra mutations causing quinolone resistance in salmonella enterica serovars typhi and paratyphi. | we developed a novel rapid assay to detect the gyra mutations that cause quinolone resistance in typhoid and paratyphoid fever salmonella spp. using high-resolution melting (idaho technology, salt lake city, ut) analysis of polymerase chain reaction amplicons. the presence of gyra mutations led to small but consistent changes in amplicon melting temperatures that allowed quinolone-resistant isolates to be differentiated from susceptible ones. | 2007 | 17156963 |
| effects of salmonella enterica serovar typhimurium, or serovar choleraesuis, lactobacillus reuteri and bacillus licheniformis on chemokine and cytokine expression in the swine jejunal epithelial cell line, ipec-j2. | direct-fed microbials, including lactobacillus and bacillus spp., are potential replacements for low dose in-feed antibiotics for swine and other livestock. to understand the function of these microbes in the gut, the current study used pig jejunal epithelial cells (ipec-j2) to evaluate how lactobacillus reuteri (lr) and bacillus licheniformis (bl) differed from salmonella enterica serovars typhimurium (st) or choleraesuis (sc) in their ability to regulate, stimulate, or modify the proinflammato ... | 2007 | 17157391 |
| characterization of salmonella enterica serovar typhimurium dt104 invasion in an epithelial cell line (ipec j2) from porcine small intestine. | salmonella typhimurium dt104 is an emerging enteric pathogen in swine of increasing medical importance. in this study, the time course and the actin-dependent host signaling processes necessary for invasion of a s. typhimurium dt104 field isolate were investigated in ipec j2 epithelial cells derived from porcine small intestine. internalized bacteria were quantified by a gentamicin resistance assay. dt104 internalization into epithelial monolayers increased steadily between 15 and 120min after a ... | 2007 | 17157450 |
| compensatory evolution reveals functional interactions between ribosomal proteins s12, l14 and l19. | certain mutations in s12, a ribosomal protein involved in translation elongation rate and translation accuracy, confer resistance to the aminoglycoside streptomycin. previously we showed in salmonella typhimurium that the fitness cost, i.e. reduced growth rate, due to the amino acid substitution k42n in s12 could be compensated by at least 35 different mutations located in the ribosomal proteins s4, s5 and l19. here, we have characterized in vivo the fitness, translation speed and translation ac ... | 2007 | 17157877 |
| pdul is an evolutionarily distinct phosphotransacylase involved in b12-dependent 1,2-propanediol degradation by salmonella enterica serovar typhimurium lt2. | salmonella enterica degrades 1,2-propanediol (1,2-pd) in a coenzyme b(12)-dependent manner. previous enzymatic assays of crude cell extracts indicated that a phosphotransacylase (ptac) was needed for this process, but the enzyme involved was not identified. here, we show that the pdul gene encodes an evolutionarily distinct ptac used for 1,2-pd degradation. growth tests showed that pdul mutants were unable to ferment 1,2-pd and were also impaired for aerobic growth on this compound. enzyme assay ... | 2007 | 17158662 |
| ssel is a salmonella-specific translocated effector integrated into the ssrb-controlled salmonella pathogenicity island 2 type iii secretion system. | bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape the outcome of disease following infection. one hallmark of the host-pathogen interaction is the prokaryotic type iii secretion system that translocates virulence factors into host cells during infection. salmonella enterica possesses two type iii secretion systems that are utilized during host colonization and intracellular replication. sal ... | 2007 | 17158898 |
| characterization of the thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase cher:mcp tethering. | sensory adaptation in bacterial chemotaxis is mediated by covalent modifications of specific glutamate and glutamine residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins (mcps). in escherichia coli and salmonella enterica, efficient methylation of mcps depends on the localization of methyltransferase cher to mcp clusters through an interaction between the cher beta-subdomain and a pentapeptide sequence (nwetf or nwesf) at the c-terminus of the mcp. in vitro methylation ... | 2007 | 17163981 |
| increasing prevalence of salmonella enterica serotype paratyphi-a in patients with enteric fever in a periurban slum setting of kolkata, india. | a sudden rise in the occurrence of salmonella enterica serotype paratyphi-a (spta) was noted in a longitudinal community-based study in kolkata, india, during 2004 - 2005. we compared the incidence rate of salmonella enterica serotype typhi (st) and spta and their antimicrobial susceptibility pattern. rate of isolation of spta was 1.5 times higher than that of st, a trend detected for the first time in that particular focus. almost all the isolates were sensitive to gentamycin and norfloxacin. m ... | 2006 | 17164172 |
| hand abscess, phlebitis, and bacteremia due to salmonella enterica serotype augustenborg. | soft tissue and superficial venous infections are uncommon extraintestinal manifestations of non-typhoidal salmonella (nts) infections. in contrast to affected adults, who usually show immunological impairment, the majority of children with extraintestinal nts infections have no predisposing risk factors. here, we report a previously healthy infant who developed hand abscess, phlebitis, bacteremia, and impending disseminated intravascular coagulation. blood and abscess cultures showed salmonella ... | 2006 | 17164956 |
| comparison of a fimbrial versus an autotransporter display system for viral epitopes on an attenuated salmonella vaccine vector. | attenuated salmonella have been used as vectors to deliver foreign antigens as live vaccines. we have previously developed an efficient surface-display system by genetically engineering 987p fimbriae to present transmissible gastroenteritis virus (tgev) c and a epitopes for the induction of anti-tgev antibodies with a salmonella vaccine vector. here, this system was compared with an autotransporter protein surface display system. the tgev c and a epitopes were fused to the passenger domain of th ... | 2007 | 17169467 |
| ac2-dpd, the bis-(o)-acetylated derivative of 4,5-dihydroxy-2,3-pentanedione (dpd) is a convenient stable precursor of bacterial quorum sensing autoinducer ai-2. | ac2-dpd, the bis-(o)-acetylated derivative of 4,5-dihydroxy-2,3-pentanedione (dpd), was prepared both as a racemic mixture and in the optically active form found in naturally occurring dpd. it was shown to exhibit the same ability as dpd to induce bioluminescence in vibrio harveyi and beta-galactosidase activity in salmonella enterica typhimurium, both gram-negative bacteria. likewise, it was also shown to inhibit biofilm formation in gram-positive bacillus cereus. the most likely hypothesis is ... | 2007 | 17169556 |
| emergence of nalidixic acid-resistant salmonella enterica serovar typhi resistant to ciprofloxacin in india. | | 2007 | 17172529 |
| a simple nucleic acid hybridization/latex agglutination assay for the rapid detection of polymerase chain reaction amplicons. | we have developed a new method for the detection of nucleic acid hybridization, based on a simple latex agglutination test that can be evaluated by the unaided eye. nucleic acid, e.g., a polymerase chain reaction (pcr) product, is denatured and incubated with polystyrene beads carrying covalently bound complementary oligonucleotide sequences. hybridization of the nucleic acids leads to aggregation of the latex particles, thereby verifying the presence of target sequence. the test is performed at ... | 2007 | 17173991 |
| vasoactive intestinal peptide (vip) prevents killing of virulent and phop mutant salmonella typhimurium by inhibiting ifn-gamma stimulated nadph oxidative pathways in murine macrophages. | vasoactive intestinal peptide is an immunomodulator with great potential in the treatment of inflammatory pathology. in this study, we have examined the effect of vip on the growth dynamics of virulent salmonella enterica. serovar typhimurium (s. typhimurium) 14028 and 4/74 and an avirulent mutant (14028 phop) in a murine, macrophage cell line (j774.2). in contrast to standard growth dynamics, in which phop mutants do not survive in macrophages, we show that vip (10(-10) m) significantly enhance ... | 2006 | 17174562 |
| outbreak of burn wound infections by salmonella enterica serovar menston and the role of disinfectant testing in finding the cause of spread. | | 2007 | 17175067 |
| antigenic competition among different 'o' antigens of salmonella enterica subspecies enterica serovars during hyperimmunization in pony mares. | the present study on antigenic competition among somatic 'o' antigens of different salmonella groups (a, b, c1, c2, d and e1) in mares revealed that the immune response to most of the antigens was not (a, b, c2) or little (c1, d) affected by antigenic competition. however, e1 group antigen, which induced high antibody titres (avg. 12967.3) when given alone, produced almost 3.5 log2 lower antibody titres on giving with other antigens, indicating the antigenic competition among some salmonella gro ... | 2006 | 17176678 |
| [an epidemiological study of outbreaks of salmonella enterica serovar paratyphi a occurred in two chinese families after traveled to different areas of china]. | we reported two familial clusters of paratyphoid fever after travel to china occurring in the same yokohama ward from september to october 2002. six salmonella enterica serovar paratyphi a (s. paratyphi a) strains, 3 each from 2 clusters, were isolated and their characteristics analyzed using phage typing, susceptibility to antibiotics, and patterns of restriction endonuclease-digested dna fragments in agarose gel following pulsed-field gel electrophoresis (pfge). mutations in genes for gyra and ... | 2006 | 17176854 |
| expression of toll-like receptors, interleukin 8, macrophage migration inhibitory factor, and osteopontin in tissues from pigs challenged with salmonella enterica serovar typhimurium or serovar choleraesuis. | two serovars of salmonella enterica, namely serovar typhimurium (st) and serovar choleraesuis (sc) account for the vast majority of clinical cases of swine salmonellosis worldwide. these serovars are thought to be transmitted among pigs in production settings mainly through fecal-oral routes. yet, few studies have evaluated effects of these serovars on expression of innate immune targets when presented to pigs via repeated oral dosing in an attempt to model transmission in production settings. t ... | 2007 | 17178162 |
| c-fos-deficient mice are susceptible to salmonella enterica serovar typhimurium infection. | c-fos is a component of transcription factor ap-1. we show that macrophages lacking c-fos exhibit enhanced production of proinflammatory cytokines, potentiated nf-kappab phosphorylation, and increased cell death following salmonella enterica serovar typhimurium infection. furthermore, mice lacking c-fos are highly susceptible to infection, suggesting that c-fos confers resistance to salmonella infection in mice. | 2007 | 17178788 |
| role of rpos in fine-tuning the synthesis of vi capsular polysaccharide in salmonella enterica serotype typhi. | regulation of the synthesis of vi polysaccharide, a major virulence determinant in salmonella enterica serotype typhi, is under the control of two regulatory systems, ompr-envz and rscb-rscc, which respond to changes in osmolarity. some serotype typhi strains exhibit overexpression of vi polysaccharide, which masks clinical detection of lipopolysaccharide o antigen. this variation in vi polysaccharide and o antigen display (vw variation) has been observed since the initial studies of serotype ty ... | 2007 | 17178790 |
| identification of putative ancestors of the multidrug-resistant salmonella enterica serovar typhimurium dt104 clone harboring the salmonella genomic island 1. | the origin of multidrug-resistant salmonella enterica serovar typhimurium (s. typhimurium) harboring the salmonella genomic island 1 (sgi1), which was detected for the first time in the mid-1980s is unknown. in this study, we performed microarray genomotyping of four multidrug-resistant sgi1 positive strains and found that unlike the s. typhimurium lt2 strain, the multidrug-resistant strains lacked genes stm0517-0529 allowing the utilization of allantoin as a sole nitrogen source. we extended th ... | 2007 | 17180672 |
| multiple nf-kappab and ifn regulatory factor family transcription factors regulate ccl19 gene expression in human monocyte-derived dendritic cells. | ccl19 chemokine has a central role in dendritic cell (dc) biology regulating dc traffic and recruitment of naive t cells to the vicinity of activated dcs. in this study, we have analyzed the regulation of ccl19 gene expression in human monocyte-derived dcs. dcs infected with salmonella enterica or sendai virus produced ccl19 at late times of infection. the ccl19 promoter was identified as having two putative nf-kappab binding sites and one ifn-stimulated response element (isre). transcription fa ... | 2007 | 17182562 |
| response of two salmonella enterica strains inoculated in model cheese treated with high hydrostatic pressure. | the aim of this work was to determine the response to high hydrostatic pressure and the ability for survival, recovery, and growth of 2 strains of salmonella enterica (salmonella enteritidis and salmonella typhimurium) inoculated in a washed-curd model cheese produced with and without starter culture. inoculated samples were treated at 300 and 400 mpa for 10 min at room temperature and analyzed after treatment and after 1, 7, and 15 d of storage at 12 degrees c to study the behavior of the salmo ... | 2007 | 17183079 |
| gata transcription factor required for immunity to bacterial and fungal pathogens. | in the past decade, caenorhabditis elegans has been used to dissect several genetic pathways involved in immunity; however, little is known about transcription factors that regulate the expression of immune effectors. c. elegans does not appear to have a functional homolog of the key immune transcription factor nf-kappab. here we show that that the intestinal gata transcription factor elt-2 is required for both immunity to salmonella enterica and expression of a c-type lectin gene, clec-67, whic ... | 2006 | 17183709 |
| role of the alternative sigma factors sigmae and sigmas in survival of salmonella enterica serovar typhimurium during starvation, refrigeration and osmotic shock. | the ability of salmonella enterica serovar typhimurium to survive environmental stress requires specific, coordinated, responses, which induce resistance to the stress condition. this study investigated the relative contribution of sigmae and sigmas, the sigma factors regulating extracytoplasmic and general stress response functions, respectively, to survival at low temperature and also in media of differing osmotic strength, conditions relevant to food preservation. to determine if low-temperat ... | 2007 | 17185555 |
| effects of different molting procedures on incidence of salmonella infection in flocks of naturally contaminated laying hens in a commercial egg-producing farm by detection of yolk antibodies to salmonella in eggs. | indirect enzyme-linked immunosorbent assays (elisas) have been applied to detect immunoglobulin y antibodies to different serotypes of salmonella in the yolks of chicken eggs with heat-extracted antigens of salmonella enterica serotypes agona (sa), cerro (sc), enteritidis (se), montevideo (sm), and putten (sp). the egg yolk samples examined were classified as positive if their elisa absorbance values exceeded the value for eggs from specific-pathogen-free flocks by more than two standard deviati ... | 2006 | 17186654 |
| heat inactivation of listeria monocytogenes and salmonella enterica serovar typhi in a typical bologna matrix during an industrial cooking-cooling cycle. | the heat resistance of salmonella enterica serovar typhi pf-724 and listeria monocytogenes 2812 was determined in a commercial bologna batter. the heat inactivation of the two bacterial species was also studied in a semiautomatic pilot smokehouse under cooking conditions that reproduced an industrial bologna process. s. enterica serovar typhi pf-724 was less heat resistant than l. monocytogenes 2812. the d-values (times required to reduce the population by 1 logarithmic cycle) for s. enterica se ... | 2006 | 17186675 |
| survival and growth of foodborne pathogens during cooking and storage of oriental-style rice cakes. | fresh cooked rice cakes for retail sale are typically held at room temperature because refrigeration dramatically reduces their quality. room temperature, high water activity, and a ph of > 4.6 provided an environment conducive to pathogen growth. to date, no studies have been published regarding survival and growth of foodborne pathogens in fresh cooked rice cakes. this study was undertaken to investigate the effect of steam cooking on foodborne pathogens and their subsequent growth in five var ... | 2006 | 17186677 |
| salmonella enterica serovar kentucky: antimicrobial resistance and molecular analysis of clinical isolates from the slovak republic. | a collection of 68 isolates of salmonella enterica serovar kentucky collected during the period 2003-2004 from humans in two geographical regions in the slovak republic was studied. the original isolate of this serovar was associated with travel to egypt, and the emergence of other isolates was due to the nosocomial spread of this strain in two hospitals. antimicrobial susceptibility testing, class i integrons content, pulsed-field gel electrophoresis (pfge) analysis and plasmid dna profiles wer ... | 2006 | 17186952 |
| use of linear, weibull, and log-logistic functions to model pressure inactivation of seven foodborne pathogens in milk. | survival curves of six foodborne pathogens suspended in ultra high-temperature (uht) whole milk and exposed to high hydrostatic pressure at 21.5 degrees c were obtained. vibrio parahaemolyticus was treated at 300 mpa and other pathogens, listeria monocytogenes, escherichia coli o157:h7, salmonella enterica serovar enteritidis, salmonella enterica serovar typhimurium, and staphylococcus aureus were treated at 600 mpa. all the survival curves showed a rapid initial drop in bacterial counts followe ... | 2007 | 17188197 |