| role of the 'cre/blr-tag' dna sequence in regulation of gene expression by the aeromonas hydrophila beta-lactamase regulator, blra. | to further understand the mechanisms used to regulate expression of the blr regulon of aeromonas hydrophila t429125, including three unlinked beta-lactamase genes, amph, ceph and imih, and to examine the role of the 'cre/blr-tag' dna sequence (ttcac) in transcriptional control exerted by the two-component system, blrab. | 2004 | 14729737 |
| association of type iii secretion genes with virulence of aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida possesses a number of potential virulence factors, including a recently identified plasmid-encoded type iii secretion system. a number of field isolates of a. salmonicida subsp. salmonicida were examined for the presence of type iii secretion genes. using in vitro experiments, it was found that field isolates containing such genes are cytotoxic to fish cell lines, whereas those that lack these genes are not. using a rainbow trout in vivo model, the virule ... | 2003 | 14735937 |
| molecular characterization of a glucose-inhibited division gene, gida, that regulates cytotoxic enterotoxin of aeromonas hydrophila. | by using a mini-transposon, we obtained two mutated strains of a diarrheal isolate, ssu, of aeromonas hydrophila that exhibited a 50 to 53% reduction in the hemolytic activity and 83 to 87% less cytotoxic activity associated with the cytotoxic enterotoxin (act). act is a potent virulence factor of a. hydrophila and has been shown to contribute significantly to the development of both diarrhea and septicemia in animal models. subsequent cloning and dna sequence analysis revealed that transposon i ... | 2004 | 14742556 |
| enteropathogenic activity and invasion of hep-2 cells by aeromonas caviae clinical isolates. | twenty aeromons caviae isolates from stool of children with diarrhea symptoms were examined for virulence-associated properties: production of cytotoxic and cytotonic toxins, and invasive ability. most of a. caviae strains were cytotoxic to vero and cho cells and produced cytotonic toxins which caused elongation of cho cells. moreover, five of a. caviae strains revealed invasive ability towards hep-2 cells. | 2003 | 14743980 |
| crystallization and preliminary x-ray analysis of the controller protein c.ahdi from aeromonas hydrophilia. | single crystals of purified homodimeric controller protein from aeromonas hydrophilia (c.ahdi) have been grown under several different conditions using vapour diffusion. x-ray diffraction data have been collected using synchrotron radiation from crystals of both the native and a selenomethionine (semet) derivative of the protein. the native crystal form belongs to space group p2(1) and data were collected to a resolution of 2.2 a. two crystal forms of the semet protein have been obtained and wer ... | 2004 | 14747712 |
| control of aeromonas on minimally processed vegetables by decontamination with lactic acid, chlorinated water, or thyme essential oil solution. | aeromonas is an opportunistic pathogen, which, although in low numbers, may be present on minimally processed vegetables. although the intrinsic and extrinsic factors of minimally processed prepacked vegetable mixes are not inhibitory to the growth of aeromonas species, multiplication to high numbers during processing and storage of naturally contaminated grated carrots, mixed lettuce, and chopped bell peppers was not observed. aeromonas was shown to be resistant towards chlorination of water, b ... | 2004 | 14751681 |
| the role of bacterial and non-bacterial toxins in the induction of changes in membrane transport: implications for diarrhea. | bacterial toxins induce changes in membrane transport which underlie the loss of electrolyte homeostasis associated with diarrhea. bacterial- and their secreted toxin-types which have been linked with diarrhea include: (a) vibrio cholerae (cholera toxin, e1 tor hemolysin and accessory cholera enterotoxin); (b) escherichia coli (heat stable enterotoxin, heat-labile enterotoxin and colicins); (c) shigella dysenteriae (shiga-toxin); (d) clostridium perfringens (c. perfringens enterotoxin, alpha-tox ... | 2003 | 14757199 |
| [virulence factors of clinical aeromonas caviae isolates]. | aeromonas caviae, an ubiquitous aquatic organism, has long been considered to be of low pathogenicity, and its virulence mechanisms are still not clearly understood. twenty-eight a. caviae isolates of clinical origin, most often monomicrobic, were identified in our university hospital over a four year period. patients, mostly immunocompromised, were: eight diarrhoeal infants, 13 diarrhoeal adults, seven bacteraemic adults. adults were frequently suffering from underlying intestinal malignancy, h ... | 2004 | 14761709 |
| aeromonas meningitis complicating medicinal leech therapy. | medicinal leeches have an important and expanding role in medicine, but infection can complicate their use. we describe a unique case of aeromonas meningitis associated with the use of leech therapy to salvage a skin flap after central nervous system surgery. | 2004 | 14765362 |
| coexpression of genetically engineered 3-ketoacyl-acp synthase iii (fabh) and polyhydroxyalkanoate synthase (phac) genes leads to short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production from glucose in escherichia coli jm109. | polyhydroxyalkanoates (phas) can be divided into three main types based on the sizes of the monomers incorporated into the polymer. short-chain-length (scl) phas consist of monomer units of c3 to c5, medium-chain-length (mcl) phas consist of monomer units of c6 to c14, and scl-mcl phas consist of monomers ranging in size from c4 to c14. although previous studies using recombinant escherichia coli have shown that either scl or mcl pha polymers could be produced from glucose, this study presents t ... | 2004 | 14766582 |
| isolation and characterization of microorganisms capable of decolorizing various triphenylmethane dyes. | various soil and sludge samples collected from the vicinity of textile dyeing industries and waste disposal sites were used for enrichment of microbial population in the presence of triphenylmethane (tpm) dye acid violet-17 (av-17). twenty-five (25) isolates were screened for their ability to decolorize av-17 dye added at a rate of 10 mgl(-1) in mineral salts medium (msm) agar plates. five bacterial isolates belonging to bacillus sp., alcaligenes sp. and aeromonas sp. were selected on the basis ... | 2004 | 14768029 |
| microbial transformation of pyrethroid insecticides in aqueous and sediment phases. | recent studies showed that synthetic pyrethroids (sps) can move via surface runoff into aquatic systems. fifty-six of sp-degrading bacteria strains were isolated from contaminated sediments, of which six were evaluated for their ability to transform bifenthrin and permethrin in the aqueous phase and bifenthrin in the sediment phase. in the aqueous phase, bifenthrin was rapidly degraded by strains of stenotrophomonas acidaminiphila, and the half-life (t1/2) was reduced from >700 h to 30 to 131 h. ... | 2004 | 14768859 |
| in vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus. | the selection of probiotics for aquaculture is usually based on their antagonism towards pathogens. however, other criteria such as growth, attachment to intestinal mucus and production of beneficial compounds should also be considered. we suggest a protocol for the isolation and selection of potential probiotic bacteria based on their in vitro growth characteristics and propose a ranking index (ri) to screen potential aquaculture probionts. we suggest that the lag period and doubling time are t ... | 2004 | 14769479 |
| the identity of proteus hydrophilus bergey et al. and proteus melanovogenes miles & halnan, and their relation to the genus aeromonas kluyver & van niel. | | 1951 | 14832418 |
| genetic diversity and population structure of aeromonas hydrophila, aer. bestiarum, aer. salmonicida and aer. popoffii by multilocus enzyme electrophoresis (mlee). | genetic diversity, genetic relationship, identification and population structure of 120 aeromonas strains (including aer. hydrophila, aer. bestiarum, aer. salmonicida and aer. popoffii) isolated from various sources were studied by analysis of 15 genetic loci by multilocus enzyme electrophoresis (mlee). all 15 loci were polymorphic, with an average of 9.4 alleles per locus and a mean genetic diversity (h) of 0.64. cluster analysis defined at h < or = 0.7 differentiated most of the taxa analysed ... | 2004 | 14871204 |
| production of transgenic medaka with increased resistance to bacterial pathogens. | cecropins, first identified in silk moth (hyalophora cecropia), are a group of antimicrobial peptides with bactericidal activity against a broad spectrum of bacteria. in this study we investigated whether (1) this group of antimicrobial peptides could exhibit bactericidal activity toward known fish bacterial pathogens and (2) expression of cecropin transgenes in transgenic medaka (oryzias latipas) could result in increasing resistance of the transgenic fish to infection by fish bacterial pathoge ... | 2002 | 14961264 |
| hemolytic and proteolytic activities of aeromonas hydrophila and aeromonas veronii biovar sobria in broth and salmon extract at different temperatures. | expression of hemolytic and proteolytic activities throughout the growth cycle was investigated with two enterotoxic aeromonad strains assigned to the species aeromonas hydrophila and aeromonas veronii biovar sobria. although growth kinetic data were dependent on strain, temperature, and substrate, maximum populations attained were higher than 9 log cfu/ml in aerated tryptone soya broth plus yeast extract (tsbye) and salmon extract within the range 4 to 28 degrees c. for both strains in tsbye, v ... | 2004 | 14968959 |
| a type iii secretion system is required for aeromonas hydrophila ah-1 pathogenesis. | aeromonas hydrophila is a gram-negative opportunistic pathogen in fish and humans. many bacterial pathogens of animals and plants have been shown to inject anti-host virulence determinants into the hosts via a type iii secretion system (ttss). degenerate primers based on lcrd family genes that are present in every known ttss allowed us to locate the ttss gene cluster in a. hydrophila ah-1. a series of genome walking steps helped in the identification of 25 open reading frames that encode protein ... | 2004 | 14977925 |
| structural studies on the r-type lipopolysaccharide of aeromonas hydrophila. | a rough strain of aeromonas hydrophila, ah-901, has an r-type lipopolysaccharide with the complete core. the following core structure was established by chemical degradations followed by sugar and methylation analyses along with esims and nmr spectroscopy: [formula: see text] where d-alpha-d-hep and l-alpha-d-hep stand for d-glycero- and l-glycero-alpha-d-manno-heptose, respectively; kdo stands for 3-deoxy-d-manno-oct-2-ulosonic acid; all monosaccharides are in the pyranose form; the degree of s ... | 2004 | 14980820 |
| isolation and characterization of integron-containing bacteria without antibiotic selection. | the emergence of antibiotic resistance among pathogenic and commensal bacteria has become a serious problem worldwide. the use and overuse of antibiotics in a number of settings are contributing to the development of antibiotic-resistant microorganisms. the class 1 and 2 integrase genes (inti1 and inti2, respectively) were identified in mixed bacterial cultures enriched from bovine feces by growth in buffered peptone water (bpw) followed by integrase-specific pcr. integrase-positive bacterial co ... | 2004 | 14982773 |
| bacterial stimulation upregulates the surface expression of the stress protein gp96 on b cells in the frog xenopus. | the presence of the soluble intracellular heat shock protein gp96 (an endoplasmic reticulum resident protein) at the surface of certain cell types is an intriguing phenomenon whose physiological significance has been unclear. we have shown that the active surface expression of gp96 by some immune cells is found throughout the vertebrate phylum including the agnatha, the only vertebrate taxon whose members (lamprey, hagfish) lack an adaptive immune system. to determine whether gp96 surface expres ... | 2003 | 14984060 |
| an aeromonas veronii biovar sobria infection with disseminated intravascular gas production. | we report a case of aeromonas veronii biovar sobria infection with disseminated intravascular gas production. the patient was an afebrile 15-year-old girl who had been quite healthy until the onset of the illness. she came to the hospital because of a 6-h history of increasing pain and swelling in her left thigh. on admission, no infection was suspected, and a tentative diagnosis of a ruptured left gracilis muscle was made. because the pain increased continuously, the treatment concentrated on p ... | 2004 | 14991516 |
| pharmacokinetics of sulfadimethoxine and ormetoprim in a 5:1 ratio following intraperitoneal and oral administration, in the hybrid striped bass (morone chrysops x morone saxitalis). | selected pharmacokinetic parameters for sulfadimethoxine and ormetoprim, administered in a 5:1 ratio, via the oral and intraperitoneal (i.p.) routes were determined in the hybrid striped bass (morone chrysops x morone saxitalis). plasma concentrations of both drugs were determined by high-performance liquid chromatography. a first-order one-compartment model adequately described plasma drug disposition. the elimination half-lives for sulfadimethoxine following i.p. and oral administration were 2 ... | 2004 | 14995959 |
| tracking spoilage bacteria in commercial poultry processing and refrigerated storage of poultry carcasses. | four trials were conducted to examine the effect of commercial processing and refrigerated storage on spoilage bacteria in the native microflora of broiler carcasses. prescalded, picked, eviscerated, and chilled carcasses were obtained from a commercial processing facility, and psychrotrophs in the bacterial flora were enumerated on iron agar, pseudomonas agar, and staa agar. the size of the population of spoilage bacteria on processed carcasses stored at 4 degrees c for 7, 10, or 14 days was al ... | 2004 | 14996459 |
| type iii secretion system genes in clinical aeromonas isolates. | we have identified the genes ascf and ascg, which encode components of a putative type iii secretion system (ttss) in aeromonas: we investigated the distribution of these and other ttss genes in 84 clinical isolates and found hybridizing sequences in 50% of the strains, with a higher prevalence in aeromonas hydrophila and aeromonas veronii than in aeromonas caviae. | 2004 | 15004096 |
| aeromonas spp associated with children's diarrhoea in tehran: a case-control study. | the study sought a possible pathogenic role for aeromonas spp in tehranian children presenting with diarrhoea. during a 10-month period (february to october 2001), 310 children <10 years of age with diarrhoea and a group of 310 age- and sex-matched controls were selected. both groups were investigated for diarrhoeal pathogens. of 310 children who presented with acute diarrhoea, aeromonas spp was isolated from 14 (4.5%) (a. veronii biovar sobria 8, a. caviae 5 and a. hydrophila 1). the isolation ... | 2004 | 15005966 |
| antimicrobial susceptibility of common bacterial pathogens isolated from a new regional hospital in southern taiwan. | antimicrobial resistance has become a major health problem in taiwan. while some trends in antimicrobial resistance are universal, others appear to be unique for specific regions. | 2003 | 15008323 |
| quantitative analysis, using maldi-tof mass spectrometry, of the n-terminal hydrolysis and cyclization reactions of the activation process of onconase. | onconase, a member of the ribonuclease superfamily, is a potent cytotoxic agent that is undergoing phase ii/iii human clinical trials as an antitumor drug. native onconase from rana pipiens and its amphibian homologs have an n-terminal pyroglutamyl residue that is essential for obtaining fully active enzymes with their full potential as cytotoxins. when expressed cytosolically in bacteria, onconase is isolated with an additional methionyl (met1) residue and glutaminyl instead of a pyroglutamyl r ... | 2004 | 15009195 |
| polymicrobic infection of hybrid striped bass (morone chrysops x morone saxatilis) with three bacterial pathogens: a case report. | | 2004 | 15009255 |
| adhesive properties of a lamb-like outer-membrane protein and its contribution to aeromonas veronii adhesion. | to identify and characterize nonfimbrial proteins from aeromonas veronii involved in the attachment to epithelial cells in vitro. | 2004 | 15012808 |
| functional significance of a periplasmic mn-superoxide dismutase from aeromonas hydrophila. | a better understanding of the role of superoxide dismutases (sods) from aeromonas hydrophila and particularly the mn-sod which shares a peculiar localization within the bacterial periplasm and is only detected during the stationary phase of growth. | 2004 | 15012822 |
| aeromonas caviae keratitis associated with contact lens wear. | we report the first case of bilateral contact lens-related aeromonas caviae keratitis associated with a. caviae contamination of the contact lens case. the presence of virulence factors produced by aeromonas species was also investigated. | 2004 | 15019388 |
| biochemical characterization and site-directed mutational analysis of the double chitin-binding domain from chitinase 92 of aeromonas hydrophila jp101. | chitinase 92 from aeromonas hydrophila jp101 contains c-terminal repeated chitin-binding domains (chbds) which were named chbd(ci) and chbd(cii) and classified into family 5 carbohydrate-binding modules on the basis of sequence. in this work, we constructed single and double chbd by use of the pet system, which expressed as isolated chbd(cii) or chbd(cicii). polysaccharide-binding studies revealed that chbd(cicii) not only bound to chitin, but also to other insoluble polysaccharides such as cell ... | 2004 | 15019735 |
| aeromonas simiae sp. nov., isolated from monkey faeces. | two aeromonas strains, ibs s6874(t) and ibs s6652, were isolated from the faeces of two healthy monkeys (macaca fascicularis) from mauritius that were kept in quarantine in the centre for primatology, strasbourg, france. phylogenetic analysis based on 16s rrna gene sequences showed that the two isolates formed an unknown genetic lineage within the genus aeromonas. the two isolates had nearly identical sequences (0.1 % nucleotide substitution) that were related closely to those of recognized aero ... | 2004 | 15023964 |
| biodegradation of acid blue-15, a textile dye, by an up-flow immobilized cell bioreactor. | acid blue-15, a complex and resonance-stabilized triphenylmethane (tpm) textile dye, resistant to transformation, was decolorized/degraded in an up-flow immobilized cell bioreactor. a consortium comprised of isolates belonging to bacillus sp., alcaligenes sp. and aeromonas sp. formed a multispecies biofilm on refractory brick pieces used as support material. the tpm dye was degraded to simple metabolic intermediates in the bioreactor with 94% decolorization at a flow rate of 4 ml h(-1). | 2004 | 15024610 |
| nosocomial infection of aeromonas hydrophila presenting as necrotizing fasciitis. | aeromonas hydrophila is infrequently reported as a causative organism of necrotizing fasciitis. we report a case of necrotizing fasciitis due to a. hydrophila in a 44-year-old man with marfan syndrome who underwent valve replacement surgery twice. he was admitted due to a 2-day history of fever. the fever was attributed to hepatitis, and ingestion of herbal medication was suspected to be the cause. the fever relapsed on the 28th day of hospitalization with rapidly progressive erythematous patche ... | 2004 | 15026859 |
| aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces. | aeromonas spp. (gram-negative, aquatic bacteria which include enteropathogenic strains) have two distinct flagellar systems, namely a polar flagellum for swimming in liquid and multiple lateral flagella for swarming over surfaces. only approximately 60% of mesophilic strains can produce lateral flagella. to evaluate flagellar contributions to aeromonas intestinal colonization, we compared polar and lateral flagellar mutant strains of a diarrheal isolate of aeromonas caviae for the ability to adh ... | 2004 | 15039313 |
| differential proteomic analysis of aeromonas salmonicida outer membrane proteins in response to low iron and in vivo growth conditions. | aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis, a serious infectious disease of salmonids. bacterial phenotypes are known to change in vivo compared to the in vitro state. proteomic analysis of in vivo phenotypes is usually not possible due to insufficient biomass. using an in vivo growth chamber model, the pathogenic fish bacterium a. salmonicida was cultured in pure culture in vivo in its host, the atlantic salmon, to obtain sufficient biomass to allow proteo ... | 2004 | 15048988 |
| expression of chitinase-encoding genes in bacillus thuringiensis and toxicity of engineered b. thuringiensis subsp. aizawai toward lymantria dispar larvae. | chitinase genes from aeromonas hydrophila and bacillus circulans no.4.1 were cloned into the plasmid phy300plk and designated as phya2 and phyb43, respectively. both plasmids were introduced into various strains of b. thuringiensis by electroporation. plasmid phyb43 was generally structurally stable, but showed lower segregrational stability than phya2 in b. thuringiensis subsp. aizawai when grown under nonselective conditions. the production of chitinase from b. thuringiensis subsp. aizawai har ... | 2004 | 15057461 |
| isolation and characterization of arsenate-reducing bacteria from arsenic-contaminated sites in new zealand. | two environmental sites in new zealand were sampled (e.g., water and sediment) for bacterial isolates that could use either arsenite as an electron donor or arsenate as an electron acceptor under aerobic and anaerobic growth conditions, respectively. these two sites were subjected to widespread arsenic contamination from mine tailings generated from historic gold mining activities or from geothermal effluent. no bacteria were isolated from these sites that could utilize arsenite or arsenate unde ... | 2004 | 15060729 |
| faecal contamination indicators, salmonella, vibrio and aeromonas in water used for the irrigation of agricultural products. | the faecal contamination indicators (total coliforms, faecal coliforms, escherichia coli, enterococci) and the genera salmonella, vibrio, aeromonas were investigated in water samples used for irrigation. during 4 months, 52 samples were taken. the methods used were: multiple tube fermentation method for faecal contamination indicators and membrane filtration techniques for salmonella, aeromonas and vibrio. two samples were positive for salmonella spp., fourteen for aeromonas spp. and no samples ... | 2004 | 15061497 |
| diversity of kenyan soda lake alkaliphiles assessed by molecular methods. | dna was extracted from water and sediment samples taken from soda lakes of the kenyan-tanzanian rift valley. dna was also extracted from microbial enrichment cultures of sediment samples. 16s rrna genes were amplified by the polymerase chain reaction and microbial diversity was studied using denaturing gradient gel electrophoresis (dgge) of 16s rdna amplicons. cloning and sequencing of single dgge bands showed that they usually contained mixed amplicons. several of the amplicon sequences had hig ... | 2004 | 15064991 |
| simultaneous detection of marine fish pathogens by using multiplex pcr and a dna microarray. | we coupled multiplex pcr and a dna microarray to construct an assay suitable for the simultaneous detection of five important marine fish pathogens (vibrio vulnificus, listonella anguillarum, photobacterium damselae subsp. damselae, aeromonas salmonicida subsp. salmonicida, and vibrio parahaemolyticus). the array was composed of nine short oligonucleotide probes (25-mer) complementary to seven chromosomal loci (cyt, rpon, gyrb, toxr, urec, dly, and vapa) and two plasmid-borne loci (fata and a.sa ... | 2004 | 15070982 |
| aeromonas hydrophila orbital cellulitis in a patient with myelodysplastic syndrome. | orbital cellulitis caused by aeromonas hydrophila developed in a 73-year-old male with a history of myelodysplastic syndrome. he was admitted because of fever, general malaise, pain as well as periorbital swelling in the right eye. four days later, a yellowish pustule with purulent material was noted over right lower eyelid. aeromonas hydrophila was isolated from the discharge. after administering intravenous cefuroxime 1,500 mg every 8 hours and topical ofloxacin eye oint, his symptoms subsided ... | 2004 | 15077892 |
| secretion of haemolysins and proteases by aeromonas hydrophila eo63: separation and characterization of the serine protease (caseinase) and the metalloprotease (elastase). | to determine the haemolysins and proteases excreted by the virulent strain eo63 of aeromonas hydrophila grown in complex media and to then fractionate and characterize them, in particular those with elastolytic activity. | 2004 | 15078516 |
| bacteriology of unshelled frozen blue swimming crab (portunus pelagicus). | in this study, 45 (10 whole specimens and 35 frozen claws) frozen samples of portunus pelagicus imported into sicily (italy) from the west coast of africa were examined to assess their bacteriological characteristics and suitability for consumption. bacteriological examination was performed on two subsamples for each whole crab. the first was the body and claw muscle; the second was a pool of viscera and gills. in the case of frozen claws, each muscle claw was a sample. an aerobic plate count at ... | 2004 | 15083736 |
| [study of antibiotic influence on adherence capacity of gram positive and gram negative bacteria to the cellular substrate]. | bacterial adherence to the cellular substrate (skin and mucosa) represents a precondition of infectious pathology. it was demonstrated that bacteria which adhere and form biofilms on catheters and other inert materials used in medicine are resistant to the therapeutic antibiotic concentrations being protected by the biofilm mathrix and generating severe and hard to treat infections. there are only few studies on the influence of antibiotics on the bacterial adhesins synthesis and bacterial adher ... | 2002 | 15085601 |
| a secreted metallo protease from aeromonas hydrophila exhibits prothrombin activator activity. | detection, purification, and partial characterization of a protease from aeromonas hydrophila capable of cleaving prothrombin into active thrombin is described. the protease has been characterized with respect to enzymatic characteristics such as optimum reaction conditions for prothrombin activation, usage of additional substrates, as well as sensitivity against inhibitors. the protease activity can reversibly be inhibited by me2+ chelating agents like ethylenediamine tetraacetic acid. the enzy ... | 2004 | 15091004 |
| outbreak of aeromonas hydrophila wound infections associated with mud football. | on 16 february 2002, a total of 26 people presented to the emergency department of the local hospital in the rural town of collie in southwest western australia with many infected scratches and pustules distributed over their bodies. all of the patients had participated in a "mud football" competition the previous day, in which there had been ~100 participants. one patient required removal of an infected thumbnail, and another required surgical debridement of an infected toe. aeromonas hydrophil ... | 2004 | 15095211 |
| 16s to 23s rdna spacer fragment length polymorphism of aeromonas sp. | we analyzed polymorphism of the pcr-amplified 16s-23s rdna spacer of aeromonas species. a total of 69 isolates representing 18 dna hybridization groups were used in this study. the analysis of pcr products of 16s-23s rdna spacers revealed patterns consisting of two to eight dna fragments. the fragment sizes ranged from 730 to 1050 bp. dna patterns revealed a considerable genetic diversity between species and within a species. when a procedure to eliminate heteroduplex formation was performed, th ... | 2003 | 15095921 |
| cefotaximases (ctx-m-ases), an expanding family of extended-spectrum beta-lactamases. | among the extended-spectrum beta-lactamases, the cefotaximases (ctx-m-ases) constitute a rapidly growing cluster of enzymes that have disseminated geographically. the ctx-m-ases, which hydrolyze cefotaxime efficiently, are mostly encoded by transferable plasmids, and the enzymes have been found predominantly in enterobacteriaceae, most prevalently in escherichia coli, salmonella typhimurium, klebsiella pneumoniae, and proteus mirabilis. isolates of vibrio cholerae, acinetobacter baumannii, and a ... | 2004 | 15105882 |
| evaluation of biofilm of aeromonas hydrophila for oral vaccination of clarias batrachus--a carnivore model. | biofilm of aeromonas hydrophila was evaluated for oral vaccination of walking catfish (clarias batrachus l.). fish were fed with fish paste incorporating biofilm (bf) or free cells (fc) of a. hydrophila for 20 days and monitored for serum antibody production up to 60 days post-vaccination. serum agglutinating antibody titre and relative percent survival (rps) following challenge were found to be significantly higher in catfish fed with bf vaccine compared to that with fc. | 2004 | 15110335 |
| sequential study of antigen persistence and concomitant inflammatory reactions relative to side-effects and growth of atlantic salmon (salmo salar l.) following intraperitoneal injection with oil-adjuvanted vaccines. | the persistence of antigens at the injection site (area around pyloric caeca and spleen), concomitant inflammatory reaction and granuloma development were monitored at 3, 6 and 12 months following intraperitoneal injection with multivalent, oil-adjuvanted vaccines in atlantic salmon. parallel assessment of side-effect profiles and growth rate were also performed. antigen persistence was examined by use of a monoclonal antibody that recognises aeromonas salmonicida lipopolysaccharide in an immuno ... | 2004 | 15110337 |
| cloning, characterisation and expression of aeromonas hydrophila major adhesin. | aeromonas hydrophila, an important pathogen in fish, is believed to cause diseases by adhesive and enterotoxic mechanisms. the adhesion is a prerequisite for successful invasion. in this study, the gene of a 43 kda major adhesin (designated as aha1) was cloned and expressed. nucleotide sequence analysis of aha1 revealed an open reading frame encoding a polypeptide of 373 amino acids with a 20-amino-acid putative signal peptide (molecular weight 40,737 da). the amino acid sequences of aha1p showe ... | 2004 | 15110338 |
| aeromonas proteolytica aminopeptidase: an investigation of the mode of action using a quantum mechanical/molecular mechanical approach. | the aminopeptidase of aeromonas proteolytica (aap) belongs to the group of metallo-hydrolases that require two divalent cations for full activity. such binuclear metal centers are found in several aminopeptidases, raising the question whether a common mechanism, at least partly, is likely. we have used a quantum mechanical/molecular mechanical (qm/mm) approach to investigate the reaction mechanism of aap. among several possibilities, one reaction path was found to be clearly the most favorable. ... | 2004 | 15122907 |
| vaccination and immune responses against atypical aeromonas salmonicida in spotted wolffish (anarhichas minor olafsen) juveniles. | to study the effect of early vaccination, wolffish juveniles of size 50 and 90 mm, respectively, were vaccinated with an oil-adjuvanted atypical a. salmonicida bacterin. vaccination resulted in significant protection after challenge with the homologous bacterial strain and specific antibody responses were demonstrated against whole bacteria as well as purified a-layer protein and lps by elisa and western blotting but individual variation in immune responses was apparent. the a-protein was the mo ... | 2004 | 15123297 |
| antigen expression in biofilm cells of aeromonas hydrophila employed in oral vaccination of fish. | total protein, s-layer protein and lipopolysaccharides (lps) of biofilm cells of aeromonas hydrophila were analysed by sds-page and compared with that of planktonic cells. in the whole cell lysate of biofilm cells, about 15 proteins were repressed while three new proteins were expressed compared to that in planktonic cells. interestingly, in biofilm cells the s-layer proteins were lost and lps showed an additional high molecular weight band compared to that in planktonic cells. we propose that t ... | 2004 | 15123309 |
| the effect of various aeromonas bestiarum vaccines on non-specific immune parameters and protection of carp (cyprinus carpio l.). | aeromonas bestiarum is one of the causal agents of motile aeromonad infection/motile aeromonad septicemia (mai/mas) in fish. infections of the bacterium is an increasing problem in commercial carp (cyprinus carpio l.) farmed in poland. non-specific immune response of the fish, vaccinated with oil-emulsified experimental vaccines containing formalin killed whole cells (wcs), formalin killed whole culture (wct) or crude lps (50 or 1250 microg per fish) were studied on days 7 and 30 after vaccinati ... | 2004 | 15123310 |
| the auxotrophic aroa mutant of aeromonas hydrophila as a live attenuated vaccine against a. salmonicida infections in rainbow trout (oncorhynchus mykiss). | an auxotrophic aroa mutant of the aeromonas hydrophila ag2 strain is a live attenuated vaccine against a. hydrophila infection in rainbow trout (oncorhynchus mykiss). the protection conferred by the live attenuated vaccine against a. salmonicida strains is reported here, and several parameters of the specific and non-specific immune response in vaccinated trout were characterised. vaccination with a dose of 10(7)cells/fish of the aroa mutant elicited significant protection against the hooke and ... | 2004 | 15123323 |
| isolation and characterization of complement component c3 from atlantic cod (gadus morhua l.) and atlantic halibut (hippoglossus hippoglossus l.). | complement component c3 was isolated from the plasma of cod (gadus morhua l.) and halibut (hippoglossus hippoglossus l.). fast protein liquid chromatography (fplc) techniques, involving ion exchange and gel filtration columns, were used. the purified proteins were analysed by sds-page which showed a two-chain structure, alpha- and beta-chains, as seen in higher vertebrates. both proteins had intra-chain thioesters located within their alpha-chains and n-terminal amino acid sequencing confirmed t ... | 2004 | 15123326 |
| behavior of an aeromonas hydrophila aroa live vaccine in water microcosms. | genetically modified auxotrophic mutants of different fish pathogens have been used as live vaccines in laboratory experiments, but the behavior of the strains after release into aquatic ecosystems has not been characterized. we previously constructed and characterized an aroa mutant of aeromonas hydrophila and studied the protection afforded by this mutant as a live vaccine in rainbow trout. in this work, we describe the survival of this strain in aquatic microcosms prepared from fish water tan ... | 2004 | 15128521 |
| identification of immune-relevant genes from atlantic salmon using suppression subtractive hybridization. | in order to probe the interaction between an invading microorganism and its host, we have investigated differential gene expression in atlantic salmon (salmo salar) experimentally infected with the pathogen aeromonas salmonicida, the causative agent of furunculosis. subtractive cdna libraries were constructed by suppression subtractive hybridization (ssh) from 3 immune-relevant tissues at 2 time points during the infection process. both forward- and reverse-subtracted libraries were generated, a ... | 2004 | 15129324 |
| universal primer pcr with dgge for rapid detection of bacterial pathogens. | a universal primer pcr (uppcr) combined with denaturing gradient gel electrophoresis (dgge) was evaluated as a method permitting the rapid detection of pathogens. the results show that this method is efficient at amplifying the conserved regions of bacterial 16s rrna genes with universal primers and can detect causative bacterial pathogens rapidly. six species of bacteria from fisheries (pseudomonas fluorescens, vibrio anguillarum, aeromonas hydrophila, vibrio fluvialis, providencia rettgeri and ... | 2004 | 15134888 |
| microbial contamination of drinking water and disease outcomes in developing regions. | drinking water is a major source of microbial pathogens in developing regions, although poor sanitation and food sources are integral to enteric pathogen exposure. gastrointestinal disease outcomes are also more severe, due to under-nutrition and lack of intervention strategies in these regions. poor water quality, sanitation and hygiene account for some 1.7 million deaths a year world-wide (3.1% of all deaths and 3.7% of all daly's), mainly through infectious diarrhoea. nine out of 10 such deat ... | 2004 | 15138046 |
| the catalytic role of glutamate 151 in the leucine aminopeptidase from aeromonas proteolytica. | glutamate 151 has been proposed to act as the general acid/base during the peptide hydrolysis reaction catalyzed by the co-catalytic metallohydrolase from aeromonas proteolytica (aap). however, to date, no direct evidence has been reported for the role of glu-151 during catalytic turnover by aap. in order to elucidate the catalytic role of glu-151, altered aap enzymes have been prepared in which glu-151 has been substituted with a glutamine, an alanine, and an aspartate. the michaelis constant ( ... | 2004 | 15138277 |
| enrichment and identification of bacteria capable of reducing chemical oxygen demand of anaerobically treated molasses spent wash. | the aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (cod) of the spent wash. | 2004 | 15139920 |
| potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. | heterotrophic plate counts (hpcs) are commonly used to assess the general microbiological quality of drinking water. drinking water quality specifications worldwide recommend hpc limits from 100 to 500 cfu ml(-1). a number of recent studies revealed evidence that these bacteria may not be as harmless as generally accepted. it appears that immuno-compromised individuals are particularly at risk. this would include the very young and very old patients with diseases such as aids and patients on the ... | 2004 | 15145586 |
| aerolysin is activated by metalloprotease in aeromonas veronii biovar sobria. | aeromonas veronii is an opportunistic human pathogen that causes diarrhoea and extraintestinal infections, such as wound infection and septicaemia. an a. veronii protease (avp) from a biovar sobria strain, aeg1, was partially purified and characterized. mature avp hydrolysed casein but not elastin, and protease activity was inhibited by metalloprotease inhibitors. nucleotide sequence analysis showed that avp belongs to the thermolysin family of proteases. an avp-deficient mutant was constructed ... | 2004 | 15150325 |
| prevalence, serotype distribution, antibiotic susceptibility and genetic profiles of mesophilic aeromonas species isolated from hospitalized diarrhoeal cases in kolkata, india. | a comprehensive study was performed to examine incidence, species distribution, drugs sensitivity, virulence genes and molecular fingerprints of aeromonas species isolated from patients with acute diarrhoea over a period of 2 years in kolkata, india. following the aerokey ii scheme, more than 95 % of strains were identified to species level. seven different species were encountered in this study, with aeromonas caviae being dominant, followed by aeromonas hydrophila and aeromonas veronii biovar ... | 2004 | 15150333 |
| aeromonas hydrophila with plasmid-borne class a extended-spectrum beta-lactamase tem-24 and three chromosomal class b, c, and d beta-lactamases, isolated from a patient with necrotizing fasciitis. | | 2004 | 15155251 |
| purification, cloning, and sequence analysis of beta-n-acetylglucosaminidase from the chitinolytic bacterium aeromonas hydrophila strain suwa-9. | a chitinolytic bacterium was isolated from lake suwa and identified as aeromonas hydrophila strain suwa-9. the strain grew well on a synthetic medium containing colloidal chitin as sole carbon source. chitin-degrading activity was induced by colloidal chitin or n-acetylglucosamine (glcnac). most of the activity, however, was not detected in culture fluid but was associated with cells. a beta-n-acetylglucosaminidase was purified after it was solubilized from cells by sonication. the purified enzy ... | 2004 | 15170113 |
| dual flagellar systems enable motility under different circumstances. | flagella are extremely effective organelles of locomotion used by a variety of bacteria and archaea. some bacteria, including aeromonas, azospirillum, rhodospirillum, and vibrio species, possess dual flagellar systems that are suited for movement under different circumstances. swimming in liquid is promoted by a single polar flagellum. swarming over surfaces or in viscous environments is enabled by the production of numerous peritrichous, or lateral, flagella. the polar flagellum is produced con ... | 2004 | 15170400 |
| chemical structure of aeromonas gum--extracellular polysaccharide from aeromonas nichidenii 5797. | aeromonas (a) gum, an extracellular heteropolysaccharide produced by the bacterium aeromonas nichidenii strain 5797, was studied by 1h and 13c nmr spectroscopy including 2d cosy, tocsy, 1h, 13c hmqc, hmbc and roesy experiments after o-deacetylation and smith degradation. these investigations revealed the presence of an o-acetylated pentasaccharide repeating unit composed of mannose, glucose, xylose and glucuronic acid, and it has the following structure: [image: see text] | 2004 | 15183737 |
| competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus. | probiotics for aquaculture are generally only selected by their ability to produce antimicrobial metabolites; however, attachment to intestinal mucus is important in order to remain within the gut of its host. five candidate probiotics (ap1-ap5), isolated from the clownfish, amphiprion percula (lacepéde), were examined for their ability to attach to fish intestinal mucus and compete with two pathogens, aeromonas hydrophila and vibrio alginolyticus. two different radioactive isotopes were used to ... | 2004 | 15189372 |
| doripenem (s-4661), a novel carbapenem: comparative activity against contemporary pathogens including bactericidal action and preliminary in vitro methods evaluations. | to investigate the potency of doripenem, a broad-spectrum carbapenem characterized by a wider spectrum of activity combining antimicrobial and bactericidal features of imipenem and meropenem. | 2004 | 15190031 |
| the usefulness of molecular techniques to assess the presence of aeromonas spp. harboring virulence markers in foods. | a total of 78 raw and 123 processed and ready-to-eat retail food samples were used to assess the presence of motile aeromonas spp. harboring virulence genes (cytotoxic enterotoxin and hemolysin genes) using a recently described pcr method in comparison with the conventional cultivation method based on the use of ampicillin-dextrin agar (ada) medium. with the ada-based method, 65/201 (32.3%) samples showed presumptive aeromonas spp. colonies whereas the pcr method revealed the presence of aeromon ... | 2004 | 15193799 |
| studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene analysis. | to describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ... | 2004 | 15210998 |
| polyphasic taxonomic study of "aeromonas eucrenophila-like" isolates from clinical and environmental sources. | in the current study, a group of 17 "aeromonas eucrenophila-like" isolates from clinical and environmental origins was subjected to a polyphasic taxonomic characterization including ribotyping, 16s rdna sequencing, faflp fingerprinting, and biochemical testing. genotypic, phylogenetic, and phenotypic results were consistent with the conclusion that this group of 17 isolates may represent a currently undescribed aeromonas taxon most closely related to a. eucrenophila hg6. in addition, comparison ... | 2004 | 15214640 |
| aeromonas hydrophila cytotoxic enterotoxin activates mitogen-activated protein kinases and induces apoptosis in murine macrophages and human intestinal epithelial cells. | a cytotoxic enterotoxin (act) of aeromonas hydrophila possesses several biological activities, induces an inflammatory response in the host, and causes apoptosis of murine macrophages. in this study, we utilized five target cell types (a murine macrophage cell line (raw 264.7), bone marrow-derived transformed macrophages, murine peritoneal macrophages, and two human intestinal epithelial cell lines (t84 and ht-29)) to investigate the effect of act on mitogen-activated protein kinase (mapk) pathw ... | 2004 | 15215244 |
| cytotoxin testing of environmental aeromonas spp. in vero cell culture. | aeromonas spp. are known to cause a variety of infections in humans and this organism has been isolated from a variety of sources including environmental sources. the pathogenicity of the environmental isolates in and around vellore has not been studied. this study was conducted to determine the cytotoxicity of the aeromonas spp. isolated from water bodies, soil sediments, plankton and sewers in and around vellore. | 2004 | 15218980 |
| correlation between production of acyl homoserine lactones and proteases in an aeromonas hydrophila aroa live vaccine. | aeromonas hydrophila is a pathogen that causes disease in a wide range of homeothermic and poikilothermic hosts due to its multifactorial virulence. we have previously described the characterisation and use of an auxotrophic aroa mutant of the a. hydrophila ag2 strain as a live attenuated vaccine against a. hydrophila infections in rainbow trout (oncorhynchus mykiss). in this study we report the expression of extracellular proteolytic activities and of quorum-sensing molecules by this mutant gro ... | 2004 | 15223121 |
| bacterial removal and protozoan grazing in biological sand filters. | the objective of the study was to investigate the importance of protozoan predation as a biological removal mechanism in sand filters used for purification of bacteria from wastewater. eleven sand filter columns were seeded with a high dose of wastewater (70 mm d(-1)) and a high concentration (10(8) colony forming units [cfu] ml(-1)) of aeromonas hydrophila (american type culture collection [atcc] 14715) for a period of 30 d. water samples from three filter outlets were analyzed for the concentr ... | 2004 | 15224942 |
| distribution of potentially pathogenic bacteria as free living and plankton associated in a marine coastal zone. | to determine the abundance of faecal and nonfaecal bacteria related to human and animal health, as free living or associated with small (>64 microm) and large (>200 microm) plankton, samples were collected monthly from the coastal zone at messina (italy). | 2004 | 15239702 |
| analysis and validation of a predictive model for growth and death of aeromonas hydrophila under modified atmospheres at refrigeration temperatures. | specific growth and death rates of aeromonas hydrophila were measured in laboratory media under various combinations of temperature, ph, and percent co(2) and o(2) in the atmosphere. predictive models were developed from the data and validated by means of observations obtained from (i) seafood experiments set up for this purpose and (ii) the combase database (http://www.combase.cc; http://wyndmoor.arserrc.gov/combase/). two main reasons were identified for the differences between the predicted a ... | 2004 | 15240265 |
| the antimicrobial activity of extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents. | the antimicrobial activity of the chloroform, diethyl ether, acetone, petroleum ether, and ethanol extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents against 9 bacteria and fungi has been investigated. the extracts and pure compounds alone were found active against the same bacteria and the same yeasts. bacillus cereus, bacillus subtilis, staphylococcus aureus, streptococcus faecalis, proteus vulgaris, listeria monocytogenes, aero ... | 2004 | 15241936 |
| bacteriology of a bear bite wound to a human: case report. | human contact with bears has become more frequent, as has the resultant bear maulings and bite injuries. we report the bacteriology of a patient bitten by a grizzly bear (ursus arctos) from the rocky mountains foothills area east of banff national park, alberta, canada. the patient received field care, including metronidazole and cefazolin. subsequent deep-wound cultures grew serratia fonticola, serratia marcescens, aeromonas hydrophila, bacillus cereus, and enterococcus durans but no anaerobes. | 2004 | 15243122 |
| the development of random dna probes specific for aeromonas salmonicida. | rapd-pcr has been used to produce dna probes for aeromonas salmonicida. dna hybridization studies showed that rapd-pcr fragments of the same size did not necessarily hybridize to each other and therefore these sequences were not always homologous. however, a single rapd-pcr fragment (designated 15e) was identified as being common to aer. salmonicida. subsequently, 15e was found to comprise five dna fragments of similar size which differed in their nucleotide sequences. all five fragments were ev ... | 1998 | 15244055 |
| the acetylcholinesterase ichthyotoxin is a common component in the extracellular products of vibrionaceae strains. | in previous work, it was reported that a strain of aeromonas hydrophila (b32) produces the most potent lethal toxin with neurotoxic activity described so far for fish. in the present study, the presence and distribution of this acetylcholinesterase toxin lethal for fish were determined in extracellular products (ecp) of 42 vibrionaceae strains using both immunological and colorimetric methods. this neurotoxin was shown to be present in the majority of the ecp from the aeromonas and vibrio strain ... | 1998 | 15244056 |
| influence of ph, salt concentration and temperature on the growth of aeromonas hydrophila. | the influence of environmental factors on the growth of aeromonas hydrophila was investigated four isolates (ah 37, ah 79, ah 86 and ah 100) were exposed to various environmental factors such as ph, salt concentration and temperature in laboratory condition. all the four isolates showed more or less similar growth at ph 7.0, 8.0 and 9.0 at 30 degrees c and 5 degrees c. at ph 5.0, 6.0 and 10.0, the log number of cells was found to be lesser than that of ph 7.0, 8.0 and 9.0 at both 30 degrees c an ... | 2003 | 15248649 |
| over-expression, purification, and characterization of metallo-beta-lactamase imis from aeromonas veronii bv. sobria. | the gene from aeromonas veronii bv. sobria encoding the metallo-beta-lactamase imis was subcloned into pet-26b, and imis was over-expressed in bl21(de3) escherichia coli and purified using sp-sepharose chromatography. this protocol yielded over 5 mg of imis per liter of growth culture under optimum conditions. the biochemical properties of recombinant imis were compared with those of native imis. recombinant and native imis have the same n-terminus of a-g-m-s-l, and cd spectroscopy was used to s ... | 2004 | 15249050 |
| clostridium perfringens epsilon-toxin shows structural similarity to the pore-forming toxin aerolysin. | epsilon-toxin from clostridium perfringens is a lethal toxin. recent studies suggest that the toxin acts via an unusually potent pore-forming mechanism. here we report the crystal structure of epsilon-toxin, which reveals structural similarity to aerolysin from aeromonas hydrophila. pore-forming toxins can change conformation between soluble and transmembrane states. by comparing the two toxins, we have identified regions important for this transformation. | 2004 | 15258571 |
| a comparison of bacillus cereus and aeromonas hydrophilia zn-beta-lactamases. | | 1999 | 15263322 |
| in vitro effect of a buffered chelating agent and neomycin or oxytetracycline on bacteria associated with diseases of fish. | the antimicrobial agents used to treat bacterial fish diseases are archaic, and their uses may result in the emergence of drug-resistant bacterial strains. this study evaluated the in vitro antimicrobial activity of combinations of tricide and neomycin or oxytetracycline on common disease-causing bacteria of fish and its possible use as an alternative treatment of these diseases. tricide solutions containing of 8 mm united states pharmacopeia (usp) disodium ethylenediaminetetraacetate dehydrate ... | 2004 | 15264722 |
| purification and characterization of a novel metalloprotease isolated from aeromonas caviae. | a novel protease produced by aeromonas caviae was purified and characterized. the molecular weight of the protease (ap19) was estimated as 19 kda on sds-polyacrylamide gel electrophoresis. the protease activity was not inhibited completely by heating at 100 degrees c for 15 min. the proteolytic activities were inhibited by metalloprotease inhibitor. the n-terminal amino acid sequence of ap19 was vtasvsfsgrctn. ap19 did not activate aeromonas proaerolysin, and did not show fluid accumulation in t ... | 2004 | 15268947 |
| aeromonas hydrophila beta-hemolysin induces active chloride secretion in colon epithelial cells (ht-29/b6). | the diarrheal mechanisms in aeromonas enteritis are not completely understood. in this study we investigated the effect of aeromonads and of their secretory products on ion secretion and barrier function of monolayers of human intestinal cells (ht-29/b6). ion secretion was determined as a short-circuit current (i(sc)) of ht-29/b6 monolayers mounted in ussing-type chambers. transepithelial resistance (r(t)) served as a measure of permeability. a diarrheal strain of aeromonas hydrophila (strain sb ... | 2004 | 15271947 |
| spectroscopic and x-ray crystallographic characterization of bestatin bound to the aminopeptidase from aeromonas (vibrio) proteolytica. | binding of the competitive, slow-binding inhibitor bestatin ([(2s,3r)-3-amino-2-hydroxy-4-phenylbutanoy]-leucine) to the aminopeptidase from aeromonas proteolytica (aap) was examined by both spectroscopic and crystallographic methods. electronic absorption spectra of the catalytically competent [co_(aap)], [coco(aap)], and [znco(aap)] enzymes recorded in the presence of bestatin revealed that both of the divalent metal ions in aap are involved in binding bestatin. the electron paramagnetic reson ... | 2004 | 15274616 |
| maintenance of pathogenicity during entry into and resuscitation from viable but nonculturable state in aeromonas hydrophila exposed to natural seawater at low temperature. | to investigate the fate of aeromonas hydrophila pathogenicity when cells switch, in nutrient-poor filtered sterilized seawater, between the culturable and nonculturable state. | 2004 | 15281936 |
| antimicrobial activity of reuterin in combination with nisin against food-borne pathogens. | antimicrobial activity of reuterin individually or in combination with nisin against different food-borne gram-positive and gram-negative pathogens in milk was investigated. reuterin (8 au/ml) exhibited bacteriostatic activity against listeria monocytogenes, whereas its activity was slightly bactericidal against staphylococcus aureus at 37 degrees c. higher bactericidal activity was detected against escherichia coli o157:h7, salmonella choleraesuis subsp. choleraesuis, yersinia enterocolitica, a ... | 2004 | 15282134 |
| population patterns and antimicrobial resistance of aeromonas in urban playa lakes. | bacteria belonging to the genus aeromonas are indigenous to aquatic environments. once regarded as unimportant human pathogens, reports of opportunistic infections caused by these organisms have appeared increasingly in the medical literature. to estimate the potential for human infection by aeromonas where limited water resources are being used intensively, we studied the spatial and temporal variation and incidence of antimicrobial resistance among environmental isolates of aeromonas from two ... | 2004 | 15284885 |
| epidemiological and clinical characteristics of bacteraemia caused by aeromonas spp. as compared with escherichia coli and pseudomonas aeruginosa. | we reviewed 75 episodes of aeromonas spp. bacteraemia observed at our institution in 1979-2002, with special reference to episodes occurring in elderly patients (> or = 65 y) and to nosocomial episodes. in addition, we compared monomicrobial bacteraemic episodes caused by aeromonas spp. (n = 54) with those caused by escherichia coli (n = 108) and pseudomonas aeruginosa (n = 108), to assess differences in clinical presentation and outcome. the 75 episodes of aeromonas spp. bacteraemia occurred ma ... | 2004 | 15287377 |