| two outbreaks of esbl-producing klebsiella pneumoniae in a neonatal intensive care unit. | in the present study, two epidemic episodes of extended spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae in the neonatal intensive care unit (nicu) were evaluated. | 2014 | 24127911 |
| synthesis and antimicrobial profile of n-substituted imidazolium oximes and their monoquaternary salts against multidrug resistant bacteria. | two different series of n-substituted imidazolium oximes and their monoquaternary salts were synthesized and biologically tested with respect to their ability to inhibit growth a diverse panel of antibiotic susceptible gram-positive and antibiotic resistant gram-negative bacteria as well fungal strains. the newly synthesized compounds were analyzed by spectral studies to confirm their structure. the preliminary results showed that all compounds tested possess promising antimicrobial potential ag ... | 2013 | 24126094 |
| pimp-ph114 carrying bla imp-4 in a klebsiella pneumoniae strain is closely related to other multidrug-resistant inca/c2 plasmids. | the inca/c plasmids are broad host-range vehicles which have been associated with wide dissemination of cmy-2 among enterobacteriaceae of human and animal origins. acquired metallo-β-lactamases (mbls) such as the imp-type enzymes are increasingly reported in multidrug-resistant gram-negative bacteria worldwide, particularly in enterobacteriaceae. we described the complete sequence of the first imp-4-encoding inca/c2 plasmid, pimp-ph114 (151,885 bp), from a sequence type 1 klebsiella pneumoniae s ... | 2014 | 24121549 |
| biodegradation of 2-methylquinoline by klebsiella pneumoniae tj-a isolated from acclimated activated sludge. | bacterial strain klebsiella pneumoniae tj-a, which was capable of utilizing 2-methylquinoline as the sole carbon and energy source, was isolated from acclimated activated sludge under aerobic conditions. effects of temperature and initial ph on the biodegradation of 2-methylquinoline by klebsiella pneumoniae tj-a were investigated. the optimal temperature and initial ph were 30°c and 7.5, respectively. the degradation process was well described by the haldane model. then 1, 2, 3, 4-tetrahydro-2- ... | 2014 | 24117081 |
| profile of infective microorganisms causing ventilator-associated pneumonia: a clinical study from resource limited intensive care unit. | ventilator-associated pneumonia (vap) is the most common cause of hospital acquired infection and death among patients admitted in icu. microorganisms responsible for vap vary from place to place. gram-negative bacteria (gnb) have emerged as a major group of pathogen causing vap and over the years carbapenem group of antibiotics has emerged as one of the important antibiotics used in the critically ill patients. there have been reports of increased occurrence of infection by carbapenem-resistant ... | 2013 | 24106362 |
| klebsiella pneumoniae meningitis induces memory impairment and increases pro-inflammatory host response in the central nervous system of wistar rats. | klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. the invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. the aim of the present study was to evaluate memory and pro-inflammatory mediators at different times in the brains of adult wistar rats with k. pneumoniae meningitis. the animals were sacrificed at 6, 12, 24, 48 and 96 h ... | 2014 | 24105840 |
| mobile genetic elements of pseudomonas aeruginosa isolates from hydrotherapy facility and respiratory infections. | the content of mobile genetic elements in pseudomonas aeruginosa isolates of a pristine natural mineral water system associated with healthcare was compared with clinical isolates from respiratory infections. one isolate, from the therapy pool circuit, presented a class 1 integron, with 100% similarity to a class 1 integron contained in plasmid p4800 of the klebsiella pneumoniae kp4800 strain, which is the first time it has been reported in p. aeruginosa. class 1 integrons were found in 25.6% of ... | 2014 | 24102722 |
| rapid emergence of secondary resistance to gentamicin and colistin following selective digestive decontamination in patients with kpc-2-producing klebsiella pneumoniae: a single-centre experience. | after a single patient was transferred to leipzig university hospital from a hospital in rhodes, greece, the hospital experienced the largest outbreak due to a kpc-2-producing klebsiella pneumoniae (kpc-2-kp) strain thus far observed in germany. ninety patients hospitalised between july 2010 and october 2012 were affected. in an attempt to eliminate kpc-2-kp from their digestive tracts, 14 consecutive patients (16%) were treated with a short course (7 days) of selective digestive decontamination ... | 2013 | 24100228 |
| increasing occurrence of antimicrobial-resistant hypervirulent (hypermucoviscous) klebsiella pneumoniae isolates in china. | new hypervirulent variants of klebsiella pneumoniae (hvkp) are emerging globally, most of which exhibit antimicrobial susceptibility. | 2014 | 24099919 |
| predominance of klebsiella pneumoniae st14 carrying ctx-m-15 causing neonatal sepsis in tanzania. | klebsiella pneumoniae strains expressing esbls are a predominant cause of hospital acquired infections. here we describe the molecular epidemiology of these isolates in a tertiary hospital in tanzania, as potential pathogens for neonatal infections. | 2013 | 24099282 |
| structural diversity of class 1 integrons and their associated gene cassettes in klebsiella pneumoniae isolates from a hospital in china. | klebsiella pneumoniae strains carrying class 1 integrons are becoming more common worldwide, and their role in the dissemination of drug resistance is significant. the aim of this study was to characterize the structural diversity of class 1 integrons and their associated gene cassettes in k. pneumoniae isolates from hospital settings. | 2013 | 24098729 |
| pyogenic liver abscess among children in a medical center in central taiwan. | pediatric pyogenic liver abscess is uncommon. this study aimed to investigate the clinical characteristics, radiologic features, pathogens, duration of hospitalization, and management of pediatric pyogenic liver abscess. | 2015 | 24094502 |
| characterization of extended-spectrum beta-lactamase-producing klebsiella pneumoniae from riyadh, saudi arabia. | the objectives of this study were to determine the prevalences, genotypes, and clonal relationships of extended-spectrum beta-lactamase (esbl)-producing strains in 98 klebsiella pneumoniae isolates from riyadh. the prevalence of esbls in these strains was 37·75%. all isolates that were confirmed to have esbls were completely resistant to amoxicillin/clavulanate, aztreonam, cefotaxime, ceftazidime, cefoxitin, and gentamicin and were susceptible to tigecycline, colistin, and imipenem. in total, 16 ... | 2014 | 24091155 |
| clinical characteristics and economic consequence of klebsiella pneumoniae liver abscess in taiwan. | klebsiella pneumoniae liver abscess (kpla) has emerged as an endemic disease in taiwan, and its prevalence has been increasing in east asian countries in the past three decades. the utilization of healthcare resources associated with kpla is assumed to be substantial, and may be of future concern. this study investigated the clinical characteristics and economic burden of kpla in taiwan in 2011 and 2012. | 2015 | 24084377 |
| production of 2-butanol from crude glycerol by a genetically-engineered klebsiella pneumoniae strain. | klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvih), keto-acid reducto-isomerase (ilvc) and dihydroxy-acid dehydratase (ilvd) from k. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adha) from lactococcus lactis. engineered k. pneumonia, ∆ldha/pbr-ibo (ilvih–ilvc–ilvd–kivd–adha), produced 2-butanol (160 mg l−1) from crude glycerol. to increase the yield of 2-butanol, we ... | 2014 | 24078128 |
| observation of 2,3-butanediol biosynthesis in lys regulator mutated klebsiella pneumoniae at gene transcription level. | microorganisms that produce 2,3-butanediol (2,3-bdo) are mostly mixed acid fermentation microorganisms, and they synthesize 2,3-bdo in order to suppress medium acidification. the 2,3-bdo operon (budbac) is activated by the lysr regulator protein derived from the budr. in this study, the effect of the budr on 2,3-bdo-biosynthesis was observed at gene transcription level. the klebsiella pneumoniae strains (wabg-deleted strain (sgsb100), budr over-expressed strain (sgsb101), and the budr-deleted st ... | 2013 | 24076264 |
| green synthesis of silver nanoparticles using glucan from mushroom and study of antibacterial activity. | this work demonstrates synthesis of silver nanoparticles (agnps) using glucan isolated from a mushroom pleurotus florida blue variant. uv-vis spectroscopy showed maximum absorbance at 425 nm due to surface plasmon resonance of agnps. average diameter of the synthesized agnps was 2.445 ± 1.08 nm as revealed from tem analysis. xrd analysis confirmed the face-centered cubic (fcc) crystalline structure of metallic silver. the synthesized agnps-glucan conjugates exhibited antibacterial activity again ... | 2013 | 24076192 |
| nationwide spread of klebsiella pneumoniae carbapenemase-2-producing k. pneumoniae sequence type 11 in taiwan. | | 2013 | 24075810 |
| successful control of an outbreak of colonization by klebsiella pneumoniae carbapenemase-producing k. pneumoniae sequence type 258 in a neonatal intensive care unit, italy. | this article reports an outbreak of colonization by klebsiella pneumoniae carbapenemase-producing k. pneumoniae (kpc-kp) sequence type (st) 258 in a neonatal intensive care unit (nicu) in palermo, italy. kpc-kp st258 was detected by an active surveillance culture programme. between 18th september and 14th november 2012, kpc-kp was isolated from 10 out of 54 neonates admitted in the outbreak period. no cases of infection were recorded. male sex was associated with colonization, whereas administra ... | 2013 | 24074641 |
| predictors of mortality in multidrug-resistant klebsiella pneumoniae bloodstream infections. | the dramatic increase of antibiotic resistance in klebsiella pneumoniae has been associated with fatal outcomes. first, bloodstream infections (bsis) caused by extended-spectrum β-lactamases (esbl) enterobacteriaceae have been associated with treatment failure, more recently bsis caused by carbapenem-resistant k. pneumoniae (cr-kp) have been reported to be fatal in approximately 50% of cases. severity of underlying disease, intensive care unit stay at infection onset, infection with esbl or cr-k ... | 2013 | 24073806 |
| carbapenem-resistant klebsiella pneumoniae sepsis in corticosteroid receipt mice: tigecycline or colistin monotherapy versus tigecycline/colistin combination. | this study compared the effect of monotherapy of colistin, tigecycline, and their combination in sepsis model of mice. oxa-48 producing carbapenem-resistant klebsiella pneumoniae (crkp) strain was used in balb/c mice. the mice were divided into competent and methylprednisolone acetate (mpa)-treated groups. each group was sub-divided into (1) colistin or (2) tigecycline monotherapy and (3) colistin/tigecycline combination therapy. after 3 hours of intraperitoneal bacterial inoculation, antimicrob ... | 2014 | 24070112 |
| microbiological and molecular characterization of extreme drug-resistant carbapenemase-producing klebsiella pneumoniae isolates. | fifteen klebsiella pneumoniae clinical isolates showing non-susceptibility to carbapenems and resistant to colistin were collected in an italian hospital. all isolates resulted negative to ampc, mbl and esbl production but positive to modified hodge test, therefore were evaluated as kpc producers. the presence of blakpc genes was verified by polymerase chain reaction (pcr) and sequencing. furthermore, molecular typing was performed by multilocus sequence typing (mlst). pcr analysis and nucleotid ... | 2017 | 24067478 |
| inhibition of klebsiella pneumoniae growth and capsular polysaccharide biosynthesis by fructus mume. | klebsiella pneumoniae is the predominant pathogen isolated from liver abscess of diabetic patients in asian countries. with the spread of multiple-drug-resistant k. pneumoniae, there is an increasing need for the development of alternative bactericides and approaches to block the production of bacterial virulence factors. capsular polysaccharide (cps), especially from the k1 and k2 serotypes, is considered the major determinant for k. pneumoniae virulence. we found that extracts of the tradition ... | 2013 | 24062785 |
| a case study comparing quantitative stability-flexibility relationships across five metallo-β-lactamases highlighting differences within ndm-1. | the distance constraint model (dcm) is an ensemble-based biophysical model that integrates thermodynamic and mechanical viewpoints of protein structure. the dcm outputs a large number of structural characterizations that collectively allow for quantified stability-flexibility relationships (qsfr) to be identified and compared across protein families. using five metallo-β-lactamases (mbls) as a representative set, we demonstrate how qsfr properties are both conserved and varied across protein fam ... | 2014 | 24061924 |
| silent destruction of aortic and mitral valve by klebsiella pneumoniae endocarditis. | klebsiella endocarditis rarely affects the native valve especially in the immunocompromised and the elderly. we report a case of klebsiella endocarditis in a 60-year-old man who had a nidus of infection on the aortic valve which led to severe aortic regurgitation. this possibly spread to the anterior mitral leaflet (aml) leading to aml perforation therefore causing moderate mitral regurgitation. the reason for this suspicion was that there was perforation of the aml in the absence of vegetation. ... | 2013 | 24057412 |
| endogenous endophthalmitis in the korean population: a six-year retrospective study. | to identify the clinical features, treatment outcomes, and prognostic factors of endogenous endophthalmitis in multiple tertiary referral centers of south korea over a 6-year period. | 2014 | 24056527 |
| first description of lung abscess caused by st23 clone capsule genotype k1 klebsiella pneumoniae. | | 2015 | 24055339 |
| klebsiella pneumoniae bacteremia and renosplenic abscesses without intestinal symptoms as the initial manifestations of non-steroidal anti-inflammatory drug-induced colitis: a rare case report. | non-steroidal anti-inflammatory drugs (nsaids), the most widely prescribed drugs in the world, can cause gastrointestinal damage, including colitis. however, the prevalence of nsaid-induced colitis is unknown because the disease is often asymptomatic. | 2013 | 24053429 |
| modulation of respiratory dendritic cells during klebsiella pneumonia infection. | klebsiella pneumoniae is a leading cause of severe hospital-acquired respiratory tract infections and death but little is known regarding the modulation of respiratory dendritic cell (dc) subsets. plasmacytoid dc (pdc) are specialized type 1 interferon producing cells and considered to be classical mediators of antiviral immunity. | 2013 | 24044871 |
| first identification of kpc-2 and vim-1 producing klebsiella pneumoniae in bulgaria. | | 2013 | 24041551 |
| transfusion reaction due to klebsiella pneumoniae-contaminated red blood cells: a case report. | | 2013 | 24033349 |
| the emergence of oxa-48- and ndm-1-positive klebsiella pneumoniae in riyadh, saudi arabia. | to investigate the emergence of ndm-, oxa-48-, and vim-producing klebsiella pneumoniae in saudi arabia. | 2013 | 24021566 |
| harvesting energy of interaction between bacteria and bacteriophage in a membrane-less fuel cell. | when a fuel and oxidant flow in laminar contact through a micro-fluidic channel, a sharp interface appears between the two liquids, which eliminate the need of a proton exchange membrane. this principle has been used to generate potential in a membrane-less fuel cell. this study use such a cell to harvest energy of interaction between a bacteria having negative charge on its surface and a bacteriophage with positive and negative charges on its tail and head, respectively. when klebsiella pneumon ... | 2013 | 24021411 |
| confronting carbapenemase-producing klebsiella pneumoniae. | the ongoing spread of carbapenemase-producing (cp) multidrug-resistant enterobacteria, primarily klebsiella pneumoniae, has undoubtedly caused a public health crisis of unprecedented dimensions. the scientific community has been struggling with these highly problematic nosocomial pathogens for more than a decade. faced with the current situation, one cannot help but wish we could have done better, earlier. however, significant steps have been and are currently being made towards a better underst ... | 2013 | 24020742 |
| nasopharyngeal bacterial colonisation in hiv-positive children in cambodia. | to determine patterns of nasopharyngeal colonisation in hiv-positive children. | 2013 | 24016033 |
| a newly isolated pseudomonas sp., epibiotic on the seaweed, padina tetrastromatica, off southeastern coast of india, reveals antibacterial action. | epibionts from the red (hypnea valentiae) and brown seaweeds (padina tetrastromatica) were rapidly isolated on zobell agar medium. all the isolates from both the seaweeds (76 numbers) were tested against five human pathogens which were resistant to at least one of the commercially available antibiotics at a minimal concentration of 10 mg. the most antibiotic productive isolate (pt19) from padina tetrastromatica was extracted and observed to inhibit klebsiella pneumoniae and pseudomonas aeruginos ... | 2013 | 24013884 |
| capsular serotypes and multilocus sequence types of bacteremic klebsiella pneumoniae isolates associated with different types of infections. | we investigated the epidemiology of different serotypes of klebsiella pneumoniae isolates causing bacteremic liver abscess (la) using multilocus sequence typing (mlst). mlst and molecular typing were performed for 41 k1 (19 la), 37 k2 (5 la), and 33 non-k1/k2 (6 la) isolates that were derived from a previous one-year k. pneumoniae bacteremia cohort. capsular serotypes and rmpa of these isolates were determined by polymerase chain reaction (pcr) methods. among the 41 k1 isolates, 39 were st23 and ... | 2014 | 24013597 |
| influence of induced ciprofloxacin resistance on efflux pump activity of klebsiella pneumoniae. | the efflux pump (ep) is one of the major mechanisms of antibiotic resistance in klebsiella pneumoniae. however, there are few reports on the effect of the abuse of antibiotic use on the activity of eps. to determine whether the use of low efficacy antibiotics has any effect on the activity of eps and induces drug resistance in k. pneumoniae, we investigated the effect of ciprofloxacin on the activity of eps in k. pneumoniae strains. | 2013 | 24009204 |
| risk factors for cardiovascular events in hospitalized patients with community-acquired pneumonia. | an increased risk of cardiovascular complications has been found in those with community-acquired pneumonia (cap). preliminary data suggest that pneumococcal pneumonia, more severe pneumonia, older age, renal disease, hypoalbuminemia, and inpatient sliding scale insulin administration contribute to risk. the objective of this study was to ascertain additional factors influencing cardiovascular events in cap. | 2013 | 24007923 |
| first outbreak of kpc-2-producing klebsiella pneumoniae sequence type 258 in a hospital in south korea. | in this study, we report the first outbreak of kpc-2-producing klebsiella pneumoniae isolates from three patients admitted to a neurosurgery department in a south korean teaching hospital. multilocus sequence typing showed that the isolates were identical to the previous kpc producers in south korea and other countries, suggesting clonal spread. | 2013 | 24006005 |
| complex metabolic network of 1,3-propanediol transport mechanisms and its system identification via biological robustness. | the bioconversion of glycerol to 1,3-propanediol (1,3-pd) by klebsiella pneumoniae (k. pneumoniae) can be characterized by an intricate metabolic network of interactions among biochemical fluxes, metabolic compounds, key enzymes and genetic regulation. since there are some uncertain factors in the fermentation, especially the transport mechanisms of 1,3-pd across cell membrane, the metabolic network contains multiple possible metabolic systems. considering the genetic regulation of dha regulon a ... | 2014 | 24002752 |
| characterization and cofactor binding mechanism of a novel nad(p)h-dependent aldehyde reductase from klebsiella pneumoniae dsm2026. | during the fermentative production of 1,3-propanediol under high substrate concentrations, accumulation of intracellular 3-hydroxypropionaldehyde will cause premature cessation of cell growth and glycerol consumption. discovery of oxidoreductases that can convert 3- hydroxypropionaldehyde to 1,3-propanediol using nadph as cofactor could serve as a solution to this problem. in this paper, the yqhd gene from klebsiella pneumoniae dsm2026, which was found encoding an aldehyde reductase (kpar), was ... | 2013 | 24002450 |
| complex interactions of klebsiella pneumoniae with the host immune system in a galleria mellonella infection model. | worldwide, klebsiella pneumoniae is an increasingly problematic opportunistic pathogen, with the emergence of carbapenem-resistant isolates of special importance. the mechanisms of virulence are poorly understood, and the current study utilized the invertebrate model galleria mellonella to investigate facets of the virulence process. a range of uk clinical isolates and reference strains was assessed in galleria by measuring survival as an end point. the clinical strains showed a range of virulen ... | 2013 | 24000226 |
| heterologous expression of aldehyde dehydrogenase from saccharomyces cerevisiae in klebsiella pneumoniae for 3-hydroxypropionic acid production from glycerol. | 3-hydroxypropionic acid (3-hp) is a commercially valuable platform compound. klebsiella pneumoniae has been concerned as an appropriate host for 3-hp production because of its robust capacity to metabolize glycerol. glycerol conversion to 3-hp in k. pneumoniae comprises two successive reactions: glycerol dehydratase catalyzes glycerol to 3-hydroxypropionaldehyde (3-hpa); aldehyde dehydrogenase catalyzes 3-hpa to 3-hp. previous studies focusing on inducible expression of aldehyde dehydrogenase ha ... | 2012 | 23997342 |
| aeromonas punctata derived depolymerase that disrupts the integrity of klebsiella pneumoniae capsule: optimization of depolymerase production. | formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health. extracellular polymeric substances encompassing biofilms offer 1000-fold greater resistance as compared to the planktonic cells. using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system. the challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of klebsi ... | 2014 | 23996303 |
| comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of imp-6, vim-2, ndm-1, sim-1, kpc-1, oxa-23, and oxa-51 carbapenemase-producing acinetobacter spp., pseudomonas aeruginosa, and klebsiella pneumoniae. | matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including sim-1 or oxa-51, within 4 hours using 20 μl of 0.5 g/l ertapenem solution as a substrate. this assay is more rapid and accurate than the modified hodge test and 3-dimensional extract bioassay. hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in gram-negative bacteria. | 2013 | 23993215 |
| comparison of 3 etest(®) methods and time-kill assay for determination of antimicrobial synergy against carbapenemase-producing klebsiella species. | increasing global antibiotic resistance has resulted in more use of antibiotic combinations. there is a lack of a gold standard for in vitro testing of these combinations for synergy or antagonism. time-kill assay (tka) may be used but is labor intensive and not practical for clinical use. etest® synergy methods are more rapid and easier to perform, but there is no agreement regarding which method is best. we tested 31 clinical genetically unique klebsiella pneumoniae carbapenemase-producing kle ... | 2013 | 23993213 |
| high incidence of new delhi metallo-beta-lactamase-producing klebsiella pneumoniae isolates in sharjah, united arab emirates. | the purpose of this study was to identify carbapenem-resistant klebsiella pneumoniae in a tertiary care hospital in sharjah emirate, to identify the plasmids carrying the carbapenemase genes and to reveal clonal relationships among the isolates. two hundred and two clinically relevant isolates collected between september 2011 and october 2012 at al-qassimi hospital, sharjah, were investigated for meropenem resistance. strains with decreased susceptibility were further tested with the modified ho ... | 2014 | 23992285 |
| infections caused by carbapenem-resistant klebsiella pneumoniae among patients in intensive care units in greece: a multi-centre study on clinical outcome and therapeutic options. | infections due to carbapenem-resistant klebsiella pneumoniae (cr-kp) have emerged as a public health problem worldwide given their spread dynamics and the limited therapeutic options. our aim was to study the clinical outcome of patients with cr-kp infections in relation to antimicrobial treatment. cr-kp infections that occurred in a 10-month period (september 2009 to june 2010) in patients admitted to 19 intensive care units all over greece were studied. a total of 127 cr-kp infections were rep ... | 2014 | 23992130 |
| editorial: strangers with candy: policing the lungs with c-type lectins. | | 2013 | 23990658 |
| community-acquired klebsiella pneumoniae liver abscess: the london experience. | | 2014 | 23990158 |
| utility of a novel multiplex taqman pcr assay for metallo-β-lactamase genes plus other taqman assays in detecting genes encoding serine carbapenemases and clinically significant extended-spectrum β-lactamases. | prompt detection of infections caused by enterobacteriaceae that produce therapeutically important β-lactamases [metallo-β-lactamases (mbls), serine carbapenemases, acquired ampc and ctx-m extended-spectrum β-lactamases (esbls)] is crucial for infection prevention and control and surveillance purposes, and, more contentiously, also for effective patient management. a novel taqman pcr assay was developed to detect genes encoding imp, vim, ndm, spm, sim and gim mbls. published pcr assays for acqui ... | 2013 | 23988718 |
| klebsiella pneumoniae renal abscess syndrome: a rare case with metastatic involvement of lungs, eye, and brain. | we describe a rare case of klebsiella pneumoniae renal abscess with metastatic spread leading to endopthalmitis, pulmonary cavitary lesions, and cerebral emboli in a 41-year-old hispanic female with diabetes mellitus who presented with a four-to-five-day history of fevers, headache, eye pain, and vomiting. she was treated with iv antibiotics and made a gradual but full recovery. | 2013 | 23984128 |
| nested case-control study of the emergence of tigecycline resistance in multidrug-resistant klebsiella pneumoniae. | we performed a nested case-control study (ratio of 1:4) on the emergence of tigecycline-resistant multidrug-resistant klebsiella pneumoniae (tr-mdrkp) isolates among patients who initially presented with a tigecycline-susceptible mdrkp isolate. out of 260 patients, 24 (9%) had a subsequent clinical culture positive for a tr-mdrkp isolate within the 90-day follow-up period. on logistic regression analyses, receipt of tigecycline (adjusted odds ratio [or], 5.06; 95% confidence interval [ci], 1.80 ... | 2013 | 23979745 |
| an unusual cause of acute abdomen-gas-forming liver abscess due to salmonella enteritidis. | gas-forming pyogenic liver abscess (gfpla) is considered to be a very severe form of pla and carries a high mortality. klebsiella pneumoniae is the most common pathogen responsible for the disease, whereas cases where salmonella is cited as the cause are very uncommon. we report the first case of a 53-year-old lady suffering from gfpla due to salmonella, who was successfully treated with surgical drainage. to the best of our knowledge, this is the first case of gfpla caused by salmonella enterit ... | 2017 | 23978428 |
| dissemination of multidrug-resistant klebsiella pneumoniae clinical isolates with various combinations of carbapenemases (ndm-1 and oxa-72) and 16s rrna methylases (arma, rmtc and rmtf) in nepal. | | 2013 | 23978353 |
| poor outcome in bacterial peritonitis is associated with dysregulated micrornas and an increased inflammatory response. | peritonitis is a common cause of surgical sepsis. the failure of the host to mount an appropriate immune response contributes to persistence of the infection. we investigated the role micrornas may play in this failed immune response. | 2013 | 23972658 |
| the high incidence of carbapenem-resistant klebsiella pneumoniae in urine from elderly hospital patients may facilitate the spread of resistant strains to the community. | almost all european countries are affected by the expansion of carbapenem-resistant enterobacteriaceae occurring during recent years. in the two hospitals of verona, italy, the incidence of carbapenem-nonsusceptible klebsiella pneumoniae (cnskp) began to increase by the first months of 2011, reached a peak in the summer of the same year, and currently is around 30%. contrary to what was reported by other hospitals and although significant percentages of cnskp were detected in respiratory samples ... | 2014 | 23962153 |
| higher prevalence of klebsiella pneumoniae extended-spectrum β-lactamase in patients on renal replacement therapy. | the prevalence of antibiotic resistance is higher in patients undergoing renal replacement therapy (rrt) than in patients who did not undergo rrt. we investigated the presence of kp (klebsiella pneumoniae) in patients who underwent rrt. all data were collected retrospectively by accessing patient medical records from 2004 to 2011 for the culture results of all patients who were positive for kp. we grouped the patients by the presence of extended-spectrum β-lactamase (esbl) into a kp esbl(-) grou ... | 2013 | 23960446 |
| oral dav131, a charcoal-based adsorbent, inhibits intestinal colonization by β-lactam-resistant klebsiella pneumoniae in cefotaxime-treated mice. | antibiotics excreted into the intestinal tract, such as broad-spectrum cephalosporins, disrupt the indigenous microflora, affect colonization resistance (cr), and promote intestinal colonization by resistant bacteria. we tested whether oral dav131, a charcoal-based adsorbent, would prevent colonization by a cefotaxime (ctx)-resistant klebsiella pneumoniae strain (pug-2) in ctx-treated mice. mice received ctx, saline, ctx and dav131, or saline and dav131 for 3 days before oral challenge with 10(6 ... | 2013 | 23959311 |
| risk factors and clinical characteristics of patients with qnr-positive klebsiella pneumoniae bacteraemia. | plasmid-mediated quinolone resistance (pmqr) caused by qnr genes has been known for 15 years. information about global distribution and prevalence of qnr genes is abundant, but clinical information concerning infections produced by these isolates and risk factors for their acquisition is limited. | 2013 | 23956373 |
| feoc from klebsiella pneumoniae contains a [4fe-4s] cluster. | iron is essential for pathogen survival, virulence, and colonization. feo is suggested to function as the ferrous iron (fe(2+)) transporter. the enterobacterial feo system is composed of 3 proteins: feob is the indispensable component and is a large membrane protein likely to function as a permease; feoa is a small src homology 3 (sh3) domain protein that interacts with feob; feoc is a winged-helix protein containing 4 conserved cys residues in a sequence suitable for harboring a putative iron-s ... | 2013 | 23955005 |
| draft genome sequence of klebsiella pneumoniae isolate pr04. | klebsiella pneumoniae pr04 was isolated from a patient hospitalized in malaysia. the draft genome sequence of k. pneumoniae pr04 shows differences compared to the reference sequences of k. pneumoniae strains mgh 78578 and ntuh-k2044 in terms of their genomic structures. | 2013 | 23950113 |
| occult pyogenic liver abscess in an adolescent with type 2 diabetes. | pyogenic liver abscess is a rare complication of diabetes, usually seen in adults greater than 50 years of age who have had diabetes for many years. we describe an 18-year-old male with type 2 diabetes found to have a pyogenic liver abscess caused by klebsiella pneumoniae, and show accompanying images from his evaluation for fever of unknown origin (fuo). we conclude that in a child or adolescent with fuo and diabetes, occult pyogenic liver abscess must be considered. | 2014 | 23949897 |
| the importance of long-term acute care hospitals in the regional epidemiology of klebsiella pneumoniae carbapenemase-producing enterobacteriaceae. | in the united states, klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae are increasingly detected in clinical infections; however, the colonization burden of these organisms among short-stay and long-term acute care hospitals is unknown. | 2013 | 23946222 |
| mutations of the ompk36 porin gene and promoter impact responses of sequence type 258, kpc-2-producing klebsiella pneumoniae strains to doripenem and doripenem-colistin. | doripenem-colistin exerts synergy against some, but not all, klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae strains in vitro. we determined if doripenem mics and/or ompk36 porin gene mutations impacted the responses of 23 sequence type 258 (st258), kpc-2-producing strains to the combination of doripenem (8 μg/ml) and colistin (2 μg/ml) during time-kill assays. the median doripenem and colistin mics were 32 and 4 μg/ml. doripenem mics did not correlate with kpc-2 expression lev ... | 2013 | 23939888 |
| isolation of a bacteriophage specific for a new capsular type of klebsiella pneumoniae and characterization of its polysaccharide depolymerase. | klebsiella pneumoniae is one of the major pathogens causing hospital-acquired multidrug-resistant infections. the capsular polysaccharide (cps) is an important virulence factor of k. pneumoniae. with 78 capsular types discovered thus far, an association between capsular type and the pathogenicity of k. pneumoniae has been observed. | 2013 | 23936379 |
| yjcc, a c-di-gmp phosphodiesterase protein, regulates the oxidative stress response and virulence of klebsiella pneumoniae cg43. | this study shows that the expression of yjcc, an in vivo expression (ive) gene, and the stress response regulatory genes soxr, soxs, and rpos are paraquat inducible in klebsiella pneumoniae cg43. the deletion of rpos or soxrs decreased yjcc expression, implying an rpos- or soxrs-dependent control. after paraquat or h2o2 treatment, the deletion of yjcc reduced bacterial survival. these effects could be complemented by introducing the δyjcc mutant with the yjcc-expression plasmid pjr1. the recombi ... | 2013 | 23935824 |
| detection of vim-34, a novel vim-1 variant identified in the intercontinental st15 klebsiella pneumoniae clone. | | 2014 | 23934739 |
| genome sequence of klebsiella pneumoniae strain atcc 25955, an oxygen-insensitive producer of 1,3-propanediol. | klebsiella pneumoniae strain atcc 25955 is a 1,3-propanediol-producing bacterium that is insensitive to oxygen. here, we present a 5.29-mb assembly of its genome sequence. we have annotated 10 coding sequences (cdss) for 1,3-propanediol fermentation and 18 cdss for glycerol uptake. the cdss related to virulence and by-product formation were also annotated. | 2013 | 23929480 |
| can a routine follow-up blood culture be justified in klebsiella pneumoniae bacteremia? a retrospective case-control study. | the need for mandatory confirmation of negative conversion in klebsiella pneumoniae bacteremia (kpb) has not been adequately addressed. we conducted a retrospective case-control study of adult patients with kpb over a 5-year period in two tertiary-care hospitals to determine the risk factors for persistent bacteremia and to reevaluate the necessity of follow-up blood culture in kpb. | 2013 | 23914899 |
| klebsiella pneumoniae peptidoglycan-associated lipoprotein and murein lipoprotein contribute to serum resistance, antiphagocytosis, and proinflammatory cytokine stimulation. | peptidoglycan-associated lipoprotein (pal), murein lipoprotein (lppa), and outer membrane protein a (ompa) are dominant outer membrane proteins (omps) that are released by gram-negative bacteria during sepsis. omps are implicated in the maintenance of cell envelope integrity. here, we characterize the roles of these omps in pathogenesis during bacteremia caused by klebsiella pneumoniae. | 2013 | 23911714 |
| ompk35 and ompk36 porin variants associated with specific sequence types of klebsiella pneumoniae. | | 2013 | 23906079 |
| outbreak of pan-susceptible klebsiella pneumoniae in a neonatal intensive care unit. | we describe the outbreak of a pan-susceptible klebsiella pneumoniae strain in a neonatal intensive care unit. a total of 7 neonates developed bacteraemia (37% attack rate), of whom 3 died (43% case fatality rate). a birth weight < 1500 g was the only statistically significant risk factor. despite an extensive environmental investigation, the source was not identified. | 2013 | 23902586 |
| cranial growth restriction, a fundamental measure for success of the endoscopy in children under 1 month of age. is it possible to improve the outcome? | endoscopic third ventriculostomy has been shown to be efficient for the treatment of non-communicating hydrocephalus. however, it is not recommended as the first option in the treatment of obstructive hydrocephalus in children under 3 months of age, because the success rate is less than 35%. | 2013 | 23895985 |
| national surveillance study on carbapenem non-susceptible klebsiella pneumoniae in taiwan: the emergence and rapid dissemination of kpc-2 carbapenemase. | the global spread and increasing incidence of carbapenem non-susceptible klebsiella pneumoniae (cnskp) has made its treatment difficult, increasing the mortality. to establish nationwide data on cnskp spread and carbapenem-resistance mechanisms, we conducted a national surveillance study in taiwanese hospitals. | 2013 | 23894478 |
| the effects of cell recycling on the production of 1,3-propanediol by klebsiella pneumoniae. | the effects of both biomass age and cell recycling on the 1,3-propanediol (1,3-pdo) production by klebsiella pneumoniae were investigated in a membrane-supported bioreactor using hollow-fiber ultrafiltration membrane module in two separate experiments. it was determined that older cells have a negative effect on 1,3-pdo production. the concentrations of by-products, such as acetic acid and ethanol, increased in cultures with older cells, whereas the concentrations of succinic acid, lactic acid a ... | 2014 | 23892658 |
| cell morphology variations of klebsiella pneumoniae induced by acetate stress using biomimetic vesicle assay. | supplementation with acetate under low levels was used as a novel approach to control the morphological development of klebsiella pneumoniae aimed to improve 1,3-propanediol (1,3-pd) production. a full range of morphological types formed from rod shape to oval shape even round shape in response to different concentrations of acetate. the cell growth and 1,3-pd productions in the shake flasks with 0.5 g/l acetate addition were improved by 9.4 and 28.37%, respectively, as compared to the control, ... | 2013 | 23892619 |
| inhibiting biofilm formation by klebsiella pneumoniae b5055 using an iron antagonizing molecule and a bacteriophage. | success of biofilm dwelling bacteria in causing persistent and chronic infections is attributed to their resistance towards antibiotics and immune defences. free iron is critical for the growth of biofilm associated bacteria. therefore in the present study, the effect of limiting iron levels by addition of divalent co[ii] ions in combination with a bacteriophage was used for preventing/disrupting klebsiella pneumoniae biofilms. | 2013 | 23889975 |
| surface changes and polymyxin interactions with a resistant strain of klebsiella pneumoniae. | this study examines the interaction of polymyxin b and colistin with the surface and outer membrane components of a susceptible and resistant strain of klebsiella pneumoniae. the interaction between polymyxins and bacterial membrane and isolated lps from paired wild type and polymyxin-resistant strains of k. pneumoniae were examined with n-phenyl-1-naphthylamine (npn) uptake, fluorometric binding and thermal shift assays, lysozyme and deoxycholate sensitivity assays, and by (1)h nmr. lps from th ... | 2014 | 23887184 |
| kpc-producing klebsiella pneumoniae enteric colonization acquired during intensive care unit stay: the significance of risk factors for its development and its impact on mortality. | a prospective observational study of 226 intensive care unit (icu) patients was conducted during a 25-month period. rectal samples were taken at day 1, 4, and 7 and, afterwards, once weekly. klebsiella pneumoniae was identified using standard techniques, whereas the presence of bla(kpc) gene was confirmed by pcr. during icu stay, 72.6% of the patients were colonized with klebsiella pneumoniae carbapenemases (kpc)-producing k. pneumoniae (kpc-kp). male gender, prior bed occupants, and patients in ... | 2013 | 23886789 |
| what should be the criteria for application of modified hodge test for carbapenemases in klebsiella pneumoniae? | | 2013 | 23883731 |
| response of differentiated human airway epithelia to alcohol exposure and klebsiella pneumoniae challenge. | alcohol abuse has been associated with increased susceptibility to pulmonary infection. it is not fully defined how alcohol contributes to the host defense compromise. here primary human airway epithelial cells were cultured at an air-liquid interface to form a differentiated and polarized epithelium. this unique culture model allowed us to closely mimic lung infection in the context of alcohol abuse by basolateral alcohol exposure and apical live bacterial challenge. application of clinically r ... | 2013 | 25485141 |
| optimization of pulse-field gel electrophoresis for subtyping of klebsiella pneumoniae. | a total of 110 strains of klebsiella pneumoniae were used to optimize pulsed-field gel electrophoresis (pfge) for subtyping of k. pneumoniae. for optimization of electrophoresis parameters (eps) of xbai-pfge, 11 isolates were analyzed with xbai digestion using three eps. the ep of a switch time of 6 to 36 s for 18.5 h gave clearest patterns and was declared the optimal ep for xbai pfge of k. pneumoniae. by software analysis and pilot study, avrii was chosen as another pfge enzyme. both xbai- and ... | 2013 | 23880721 |
| comparison of in vitro efficacy of ertapenem, imipenem and meropenem in the infections caused by the enterobacteriaceae strains family. | the members of the bacterial enterobacteriaceae family play an important role in the aetiology of many hospital infections. some of them are able to produce β-lactamase, an enzyme which induces the resistance of those bacteria to penicillins, cephalosporins and, in severe infections, to penicillins with β-lactamase inhibitors. in this situation, the carbapenems become the drugs of choice. the objective of this study was to analyse the in vitro efficacy of three carbapenems: ertapenem, imipenem a ... | 2014 | 23877897 |
| identification of extended-spectrum-β-lactamase-positive klebsiella pneumoniae urinary tract isolates harboring kpc and ctx-m β-lactamases in nonhospitalized patients. | forty-seven extended-spectrum-β-lactamase-positive klebsiella pneumoniae urinary tract isolates from nonhospitalized patients were identified, and 79% harbored kpc and/or ctx-m β-lactamases. approximately 90% of the isolates were resistant to trimethoprim-sulfamethoxazole and levofloxacin, and 40% were resistant to a carbapenem, while 92% were susceptible to polymyxin b, 87% were susceptible to tigecycline, and 79% were susceptible to fosfomycin. increased use of broader-spectrum antibiotics may ... | 2013 | 23877699 |
| the regulation of 2,3-butanediol synthesis in klebsiella pneumoniae as revealed by gene over-expressions and metabolic flux analysis. | a variety of microorganism species are able naturally to produce 2,3-butanediol (2,3-bdo), although only a few of them are suitable for consideration as having potential for mass production purposes. klebsiella pneumoniae (k. pneumoniae) is one such strain which has been widely studied and used industrially to produce 2,3-bdo. in the central carbon metabolism of k. pneumoniae, the 2,3-bdo synthesis pathway is dominated by three essential enzymes, namely acetolactate decarboxylase, acetolactate s ... | 2014 | 23872849 |
| detection of new shv-12, shv-5 and shv-2a variants of extended spectrum beta-lactamase in klebsiella pneumoniae in egypt. | klebsiella pneumoniae outbreaks possessing extended-spectrum β-lactamase- (esbl) mediated resistance to third-generation cephalosporins have increased significantly in hospital and community settings worldwide. the study objective was to characterize prevalent genetic determinants of tem, shv and ctx-m types esbl activity in k. pneumoniae isolates from egypt. | 2013 | 23866018 |
| photo quiz: positive blood culture in a patient with sickle cell crisis. answer: klebsiella pneumoniae bacteremia showing filamentous forms and spheroplasts due to the presence of subinhibitory concentrations of β-lactams. | | 2013 | 23861544 |
| structural basis of a physical blockage mechanism for the interaction of response regulator pmra with connector protein pmrd from klebsiella pneumoniae. | in bacteria, the two-component system is the most prevalent for sensing and transducing environmental signals into the cell. the pmra-pmrb two-component system, responsible for sensing external stimuli of high fe(3+) and mild acidic conditions, can control the genes involved in lipopolysaccharide modification and polymyxin resistance in pathogens. in klebsiella pneumoniae, the small basic connector protein pmrd protects phospho-pmra and prolongs the expression of pmra-activated genes. we previou ... | 2013 | 23861396 |
| septic arthritis subsequent to urosepsis caused by hypermucoviscous klebsiella pneumoniae. | we herein report the first case of septic arthritis caused by rmpa-positive hypermucoviscous community-acquired k. pneumoniae that followed urosepsis in a 65-year-old japanese woman. the patient responded well to drainage of the abscesses and treatment with cefazolin. although this virulent phenotype of k. pneumoniae has been primarily reported in hong kong, we confirmed that 18/50 isolates obtained in our hospital over the past five years displayed the hypermucoviscous phenotype. therefore, cli ... | 2013 | 23857101 |
| role of interleukin 1 receptor like 1 (st2) in gram-negative and gram-positive sepsis in mice. | interleukin 1 receptor-like 1 (st2) has been implicated as a negative regulator of toll-like receptor signaling. we here sought to elucidate the role of st2 in cytokine release and systemic infection caused by two common human sepsis pathogens, streptococcus pneumoniae (gram-positive) and klebsiella pneumoniae (gram-negative). | 2013 | 23856919 |
| kpc-producing, multidrug-resistant klebsiella pneumoniae sequence type 258 as a typical opportunistic pathogen. | the virulence of a kpc-producing klebsiella pneumoniae sequence type 258 (st258) strain representing those circulating in greece was assessed in a mouse septicemia model. the strain was virtually avirulent (50% lethal dose, >10(8) and 5 × 10(7) cfu for immunocompetent and neutropenic animals, respectively). also, it was highly susceptible to serum killing, rapidly phagocytosed in vitro, and classified as k41, which is not among the virulent capsular types. the findings indirectly support the not ... | 2013 | 23856769 |
| prevalence of plasmid-mediated qnr determinants and gyrase alteration in klebsiella pneumoniae isolated from a university teaching hospital in malaysia. | the ciprofloxacin resistance of klebsiella (k.) pneumoniae is mediated primarily through alterations in type ii topoisomerase (gyra) gene and plasmid-mediated quinolone resistance-conferring genes (qnr). this study aimed to define the prevalence of plasmid-mediated quinolone resistance-conferring genes (qnr) and type ii topoisomerase (gyra) alterations of a population of ciprofloxacin-resistant (n = 21), intermediate (n = 8), and sensitive (n = 18) k. pneumoniae isolates obtained from a teaching ... | 2013 | 23852897 |
| outbreak caused by an ertapenem-resistant, ctx-m-15-producing klebsiella pneumoniae sequence type 101 clone carrying an ompk36 porin variant. | although numerous studies have documented outbreaks of carbapenem-resistant klebsiella pneumoniae (crkp) possessing various carbapenemases, reports on outbreaks due to crkp possessing extended-spectrum β-lactamases (esbls) and/or ampcs with porin lesions have been limited. here, we describe an outbreak caused by an ertapenem-resistant, ctx-m-15-producing clonal k. pneumoniae strain expressing an ompk36 porin variant. from may 2012 to november 2012, 37 ertapenem-resistant k. pneumoniae isolates p ... | 2013 | 23850951 |
| virulence of klebsiella pneumoniae isolates harboring bla kpc-2 carbapenemase gene in a caenorhabditis elegans model. | klebsiella pneumoniae carbapenemase (kpc) is a carbapenemase increasingly reported worldwide in enterobacteriaceae. the aim of this study was to analyze the virulence of several kpc-2-producing k. pneumoniae isolates. the studied strains were (i) five kpc-2 clinical strains from different geographical origins, belonging to different st-types and possessing plasmids of different incompatibility groups; (ii) seven transformants obtained after electroporation of either these natural kpc plasmids or ... | 2013 | 23844109 |
| a combined disk test for direct differentiation of carbapenemase-producing enterobacteriaceae in surveillance rectal swabs. | carbapenemase-producing enterobacteriaceae (cpe) are rapidly spreading worldwide. early detection of fecal cpe carriers is essential for effective infection control. here, we evaluated the performance of a meropenem combined disk test (cdt) for rapidly differentiating cpe isolates directly from rectal swabs. the screening method was applied for 189 rectal swabs from hospitalized patients at high risk for cpe carriage. swabs were suspended in 1 ml saline and cultured for confluent growth onto a m ... | 2013 | 23843486 |
| a nosocomial outbreak of kpc-2-producing klebsiella pneumoniae in a chinese hospital: dissemination of st11 and emergence of st37, st392 and st395. | in china, klebsiella pneumoniae carbapenemase (kpc) -producing k. pneumoniae isolates have been identified. however, little is known about the spread and outbreak of kpc-producing enterobacterial pathogens. in this study, 48 non-duplicated kpc-producing isolates were analysed for genetic relatedness by pulsed-field gel electrophoresis (pfge), antimicrobial susceptibility by e-test, and sequence type (st) by multilocus sequence typing. s1-pfge and southern blot were used for plasmid profiling, an ... | 2013 | 23841705 |
| prevalence and risk factors for acquisition of carbapenem-resistant enterobacteriaceae in the setting of endemicity. | to describe the epidemiology of carbapenem-resistant enterobacteriaceae (cre) carriage and acquisition among hospitalized patients in an area of cre endemicity. | 2013 | 23838221 |
| klebsiella pneumoniae sepsis with unusual cutaneous presentation of generalized pustulosis. | klebsiella pneumoniae is a well-known gram-negative pyogenic pathogen that can cause various types of infection. liver abscesses caused by community-acquired k. pneumoniae infection are commonly reported in taiwan, especially in people with diabetes mellitus. meningococcal bacteraemia can present as disseminated pustules and leucocytoclastic vasculitis, but it has rarely been seen in patients with k. pneumoniae infection. to date, there are only two reports in the english literature about k. pne ... | 2013 | 23837936 |