| hepatitis b virus (hbv) markers and hbv-dna in serum and liver tissue of patients with acute exacerbation of chronic type b hepatitis. | we analysed the serum samples and the liver biopsies of six consecutive chronic hbsag/anti-hbe carriers admitted to hospital because of an episode of acute hepatitis. the six patients became positive for igm anti-hbc and negative for hbeag, hepatitis delta virus (hdv) markers, igm anti-hepatitis a virus (hav), anti-cytomegalovirus (cmv) and anti-epstein-barr virus (ebv). two patients showed positivity for hepatitis b virus (hbv)-dna in serum obtained on admission, with no positivity in the subse ... | 1990 | 2365978 |
| superoxide dismutase activity and chromosome damage in cultured chromosome instability syndrome cells. | the basal levels of superoxide dismutase (sod) activity and chromosome aberration (ca) and sister-chromatid exchange (sce) frequencies were examined in cultured fibroblasts or epstein-barr virus (ebv)-transformed lymphoblastoid cell lines (lcls). these cells were derived from patients with chromosome instability syndromes (ciss) including bloom's syndrome (bs), fanconi's anemia (fa) and ataxia telangiectasia (at). embryonal fibroblasts and lcls from normal subjects served as controls. although l ... | 1990 | 2366819 |
| fc receptor gene translocation in a t(1;19) pre-b all cell line. | we have recently mapped the human fcgr2 gene to chromosome 1 bands q23-q24. in situ hybridization of fcgr2 cdna with a cell line containing a t(1:19)(q23;p13) derived from a patient with pre-b all has allowed a more accurate localization of this gene to chromosome 1 band q23. furthermore, this study indicated a splitting of the fcgr2 gene or gene cluster by the t(1;19). however, southern analysis showed no genetic rearrangement when compared with a karyotypically normal epstein-barr virus (ebv)- ... | 1990 | 2370080 |
| subcellular localization of low-abundance human immunodeficiency virus nucleic acid sequences visualized by fluorescence in situ hybridization. | detection and subcellular localization of human immunodeficiency virus (hiv) were investigated using sensitive high-resolution in situ hybridization methodology. lymphocytes infected with hiv in vitro or in vivo were detected by fluorescence after hybridization with either biotin or digoxigenin-labeled probes. at 12 hr after infection in vitro, a single intense signal appeared in the nuclei of individual cells. later in infection, when cytoplasmic fluorescence became intense, multiple nuclear fo ... | 1990 | 2371279 |
| thymus cancer epidemiology in england and wales. | thymus cancer epidemiology has been little investigated, but recent clinical studies have suggested an association with the epstein-barr virus. we studied thymus cancer incidence 1963-83 and mortality 1959-86 in england and wales, using data from the national cancer register and national mortality files. mean age-standardised incidence rates of the tumour were 0.72 per million per annum for males and 0.64 for females; mortality rates were about half of this: 0.43 for males and 0.29 for females. ... | 1990 | 2372494 |
| the role of humoral and cellular immunity in patients developing human anti-murine immunoglobulin antibody responses after radioimmunotherapy. | epstein-barr virus (ebv)-immortalised b cell lines were established from patients receiving multiple administrations (two or more) of radiolabelled murine monoclonal antibodies for the treatment of ovarian cancer. cells secreting anti-id2 ig, an immunoglobulin with binding specificities comparable to the administered murine monoclonal antibody, were isolated by using magnetic beads coated with tumour-associated antigen, incubated with the cells and concentrated with a magnetic particle concentra ... | 1990 | 2383486 |
| human monoclonal antibodies specific for the rabies virus glycoprotein and n protein. | human monoclonal antibodies to rabies virus were established by epstein-barr virus infection of peripheral blood lymphocytes collected from a rabies-vaccinated donor, and fusion with a heteromyeloma line. two human monoclonal antibodies, hum1 and hum2, both igg2, reacted with the envelope glycoprotein of the rabies virus. the antibody hum1 neutralized rabies virus (lyssavirus serotype 1) and mokola virus (lyssavirus serotype 3), but did not neutralize european bat lyssavirus, suggesting that som ... | 1990 | 2391499 |
| morphology and membrane antigens of nonlymphoid accessory cells in oral hairy leukoplakia. | we have investigated the features and distribution of accessory cells (acs) and the relationship of these cells to each other and to lymphocytes in the epithelium and lamina propria of oral hairy leukoplakia (hl), with the objective of better defining the differentiation and mutual interactions of immune-response cells within hl as a preliminary step to understanding the onset and significance of this lesion during human immunodeficiency virus (hiv) infection. twenty-four hiv-infected patients w ... | 1990 | 2394434 |
| [recent advances in clinical research on t-cell lymphoma]. | immunophenotypic analysis on 34 cases of t-cell malignancies using monoclonal antibodies against t-cell receptors (tcr) revealed 25 cases of alpha beta-type and two of gamma delta-type. the two patients with gamma delta-type showed cutaneous involvement of tumor cells. immunoblastic lymphadenopathy (ibl)-like t-cell lymphoma is divided into three histologic categories; inconspicuous type, patchy type and diffuse type. dna hybridization analysis revealed that 11 of 16 cases showed clonal rearrang ... | 1990 | 2395207 |
| polyradiculoneuritis and inappropriate adh secretion in infectious mononucleosis. | a 59-yr-old female with acute infectious mononucleosis developed a severe polyradiculoneuritis with quadriplegia and a transient syndrome of inappropriate antidiuretic hormone secretion. both these complications of the epstein-barr virus infection are rare. | 1990 | 2395492 |
| in vitro cytotoxicity of vp-16-213 and nitrogen mustard: agonistic on tumor cells but not on normal human bone marrow progenitors. | the possible presence of tumor cells in remission bone marrow (bm) is one of the major problems for the success of autologous bm transplantation (abmt), because the reinfusion of viable malignant cells may result in relapse. in this study we attempted the purging of the malignant cells by the use of vp-16-213 (vp-16) and nitrogen mustard (nm) either alone or in combination. four cell lines from various hematological malignancies were utilized: sk-dhl-2 was established from a b-cell diffuse histi ... | 1990 | 2397748 |
| [the functional activity of the natural killers in patients with infectious mononucleosis and other viral infections]. | the natural killer activity (nka) and content of large granule-containing lymphocytes (lgl) were determined in 50 patients with infectious mononucleosis and in 36 patients with other viral infections. results indicate that in 27 of 50 patients with infectious mononucleosis the number of lgl was less than 5% or equalled o. in 12 patients with infectious mononucleosis the number of lgl was 5% and in 11 children it was 5-20%. in children with different viral infections not related to the epstein-ba ... | 1990 | 2399702 |
| fulminant hepatic failure caused by acute fatty liver of pregnancy treated by orthotopic liver transplantation. | a previously healthy 35-year-old woman was seen at 37 weeks' gestation with a 10-day history of fever, vomiting, diarrhea and malaise. serum laboratory findings included elevation of serum bilirubin and ast, prolongation of serum prothrombin time and a positive monospot. a tentative diagnosis of acute fatty liver of pregnancy was made, and a healthy male infant was delivered by emergency cesarean section because of fetal distress. over the subsequent 3 days, acute progressive oliguric renal fail ... | 1990 | 2403963 |
| a serum-free culture method for myeloma progenitors in vitro: proliferative and immunophenotypic characteristics. | methods for forming multiple myeloma (mm) colonies are difficult because nonproliferative, but viable, plasma cells can survive for several weeks in culture and because mm cells tend to clump readily, forming pseudo-colonies. the present study describes a method for growing pure myeloma colonies in serum-free conditions in which genuine myeloma growth is unequivocally demonstrated. growth was observed in 17 of 32 mm bone marrow samples. after a delay of 3-5 weeks, during which most cells died, i ... | 1990 | 2406157 |
| b cells in thymomas. | although previous studies demonstrated thymoma-associated lymphocytes to be t cells, six thymomas were identified with germinal centers (gc) inside the tumor capsule. three were mixed lymphocytic and epithelial thymomas. these tumors contained gc interspersed throughout and were not associated with myasthenia gravis (mg) or with gc in adjacent thymus. three other tumors were lymphocytic thymomas with subcapsular gc. these thymomas occurred in mg patients and were associated with gc in adjacent n ... | 1990 | 2408037 |
| isoprinosine enhances the activation of sensitized lymphocytes by epstein-barr virus antigens. | isoprinosine, a synthetic purine derivative and a potentially useful immunostimulating agent, was tested for its ability to enhance lymphocyte responses to epstein-barr virus (ebv) antigens and to autologous ebv-transformed lymphocytes. isoprinosine significantly enhanced the response of sensitized lymphocytes (i.e. from ebv-seropositive individuals) to ebv antigens, while it has no effect on the lack of response of unsensitized lymphocytes (i.e. from ebv-seronegative individuals) to these antig ... | 1985 | 2409036 |
| immunostimulation by cytomegalovirus (cmv): helper t cell-dependent activation of immunoglobulin production in vitro by lymphocytes from cmv-immune donors. | cytomegalovirus (cmv) is the cause of a number of different diseases ranging from self-limited benign infections in healthy adults to life threatening illnesses among immunocompromised hosts and newborns. suppression of cell-mediated immunity is often found in cases of acute cmv infection, and in addition, the virus may also be a potent stimulant of lymphoid cells in vivo. we studied cellular proliferation and immunoglobulin (ig) production induced by cmv to determine its effect on human lymphoc ... | 1985 | 2409150 |
| prognostic value of b-cell mitogen-induced and spontaneous thymidine uptake in vitro in chronic b-lymphocytic leukaemia cells. | blood lymphocytes from 50 patients with chronic b-lymphocytic leukaemia (b-cll) were cultured in vitro with and without the polyclonal b-cell activators (pba) dextran sulphate (dxs), lipopolysaccharide from e. coli (lps), and epstein barr virus (ebv). patients with blood lymphocytes that showed a high spontaneous or pba-induced 3h-thymidine uptake in 4 d cultures had a significantly shorter therapy-free survival than patients whose lymphocytes showed a low thymidine uptake. the dxs-induced cellu ... | 1985 | 2410008 |
| polymorphisms within the hla-drw6 haplotype. ii. protein charge heterogeneity reflects mlc subtyping of hla-drw6 homozygous cells. | we have analyzed the hla class ii gene products from hla-drw6 homozygous cells. epstein-barr virus-transformed b-cell lines were internally labeled with [35s]-methionine. an np-40 lysate of the cells was subjected to immunoprecipitation, first with a drw52-like-specific monoclonal antibody and subsequently with a dr-specific framework antibody. the dr region-encoded gene products were analyzed by one-dimensional gel isoelectric focusing and two-dimensional gel electrophoresis. it is shown that d ... | 1985 | 2410359 |
| identification and characterization of the epstein-barr virus receptor on human b lymphocytes and its relationship to the c3d complement receptor (cr2). | in pursuing studies on the early events in the infection of human b cells by epstein-barr virus (ebv), we examined the host cell attachment phase with a panel of b-cell-specific monoclonal antibodies. one of the monoclonal antibodies, okb7, directly blocked the attachment of purified ebv to b lymphocytes in the absence of a second anti-immunoglobulin antibody and thereby prevented ebv infection of tonsil and peripheral blood b cells. although earlier studies have shown a close association of the ... | 1985 | 2410629 |
| a novel leukemia t-cell line (pf-382) with phenotypic and functional features of suppressor lymphocytes. | a human leukemia t-cell line (pf-382) spontaneously derived from the pleural effusion of a child with t-cell acute lymphoblastic leukemia is described. the cell line, which has been maintained in culture for over 10 months, has a modal number of 46 chromosomes and is characterized by a chromosomal abnormality, present in most of the cells, consisting of a translocation between chromosome x and chromosome 15 (46x,xq-,15p+). the cells are not recognized by the okt3 and okt11 monoclonal antibodies ... | 1985 | 2410652 |
| purification to homogeneity and characterization of human b-cell differentiation factor (bcdf or bsfp-2). | human b-cell differentiation factor (bcdf) was purified to homogeneity by sequential filtration and chromatography of culture supernatants from tcl-na1 cells on an aca34 gel column and then on a mono p column with fast protein liquid chromatography and reversed-phase hplc. a 5300-fold enrichment in specific activity of bcdf with about 25% recovery was attained. the homogeneity of purified bcdf was evidenced by the following: (i) the specific activity was 1.7 x 10(7) units/mg of protein, (ii) onl ... | 1985 | 2410927 |
| a shared antigenic determinant between human b lymphocytes and carcinomas (blca). | we have raised a murine igm monoclonal antibody (ma6) against immunoprecipitate obtained by reacting the serum of an npc patient with extract of the burkitt lymphoma cell line, raji. it reacts against an antigen (blca) which is broadly represented on human b lymphoid tissues and cell lines but is different from the functional b-cell markers such as surface immunoglobulins, ia products, fc and complement receptors. blca was found to occur on lymphoid cell lines representing all stages of b-cell d ... | 1985 | 2411675 |
| carboxyl-terminal domain of the epstein-barr virus nuclear antigen is highly immunogenic in man. | the carboxyl-terminal one-third of the epstein-barr virus nuclear antigen (ebna-1) encoded by the bamhi restriction fragment k was synthesized in escherichia coli by use of a high-expression plasmid. the resultant 28-kda ebna fusion polypeptide, comprising 5-10% of the total soluble bacterial protein, was purified to apparent homogeneity by phosphocellulose and hydroxylapatite column chromatography. both rabbit monospecific antibodies and mouse monoclonal antibodies against 28-kda ebna gave nucl ... | 1985 | 2412231 |
| cytomegalovirus in the mononucleosis syndrome in children. | in 7 children aged 18 months to 7 years isolated from a group of 128 children with infectious mononucleosis the cytomegalovirus infection was found. infection was diagnosed by determination of antibodies against immediate early and late cmv antigen by means of the elisa test. besides that, antibodies were determined against the capsid antigen and early antigen of eb virus by the method of indirect immunofluorescence. in four children only cytomegalovirus infection was found and three had a mixed ... | 1985 | 2412401 |
| biotin-labeled dna probes for detection of epstein-barr virus by in-situ cytohybridization. | conventional laboratory diagnosis of ebv-related disease is now performed by one of three methods: serology, lymphocyte transformation assay, or epstein-barr nuclear antigen (ebna) staining of cell preparations. of these techniques, serology is the most widely used. however, this approach assumes an intact host immune system, which is absent or impaired in some of the more baffling ebv-related syndromes. detection of infectious virus by the lymphocyte transformation assay is labor-intensive, req ... | 1985 | 2412753 |
| effects of x-irradiation and sodium butyrate on cell-cycle traverse on normal and radiosensitive lymphoblastoid cells. | we have used a multi-parameter flow-cytometric technique to analyse changes in cell-cycle phase distribution (early and late g1, s and g2+m phases) for normal and x-ray-sensitive (ataxia-telangiectasia, a-t) lymphoblastoid cells exposed to x-irradiation and sodium butyrate (either alone or in combination). sodium butyrate, an inhibitor of histone deacetylase, is a useful pharmacological tool for determining the proposed role of a histone acetylation-based chromatin surveillance system in control ... | 1985 | 2412868 |
| monoclonal human anti-dna antibodies from eb virus-transformed lymphocytes of systemic lupus erythematosus (sle) patients. | sixteen monoclonal human anti-dna antibodies were obtained from epstein-barr virus-transformed lymphoblastoid cells of patients with systemic lupus erythematosus (sle) and were studied in terms of antigenic specificity. all of the antibodies showed polyspecificity to polynucleotides. among them, some antibodies had a specificity to single-stranded (ss) dna. especially, o-8 antibodies showed a preference for polynucleotides with pyrimidine bases. the binding specificity of the antibody was also s ... | 1985 | 2413066 |
| cdna of the immunoglobulin kappa chain of an epstein-barr virus-transformed human lymphoid cell line: partial sequence determination and bacterial expression. | we report the isolation, nucleotide sequence determination, and bacterial expression of a partial cdna for the immunoglobulin kappa chain from the epstein-barr virus-transformed human lymphoid cell line gm131. the cdna, cloned in pbr322 by use of oligo(dg) x oligo(dc) tails, yields two pst i fragments of 250 and 600 base pairs (bp). various restriction enzyme fragments of the cdna were subcloned in the vectors m13 mp10 and m13 mp11 for sequence analysis. as a result of instability of the 250-bp ... | 1985 | 2413453 |
| long-term culture and characterization of alloreactive t-cell infiltrates from renal needle biopsies. | long-term t-cell lines have been established by culturing renal needle biopsies from kidney recipients undergoing graft rejection. these cultures are maintained in the presence of interleukin 2, with weekly addition of irradiated donor lymphocytes as a source of antigen. the cells express t-cell markers, t3, t4, and t8, t-cell growth factor (il2) receptor, and hla-dr antigens. in one cell line, j2, the t4 and t8 antigens are found on two separate cell populations. this line is also shown to spec ... | 1985 | 2414252 |
| establishment and characterization of a new human eosinophilic leukemia cell line. | a human eosinophilic leukemia cell line, designated as eol, was established from the peripheral blood of a patient with philadelphia chromosome-negative eosinophilic leukemia (el). the eol cell line grows in single cell suspension with a doubling time of 48 hours for about one year. the reactivity of these cells was tested with a panel of monoclonal antibodies; they were found to express surface ia antigen, myeloid antigen (if10, my9) and membrane receptors for interleukin 2 (il-2, tac antigen). ... | 1985 | 2415185 |
| two monoclonal antibodies defining unique antigenic determinants on human b-lymphoma cells. | the non-hodgkin's lymphomas are a clinically, morphologically, and immunologically heterogeneous group of diseases. why lymphoma cells are unresponsive to normal regulatory growth controls and how they differ from normal lymphocytes are not well understood. in order to address these questions we have raised monoclonal antibodies to neoplastic b-cells. two of these, lm-26 and lm-155, show a high degree of specificity for b-cell lymphomas. when tested by fluorescence activated cell sorter analysis ... | 1985 | 2415242 |
| isolation and characterization of ni-specific t cell clones from patients with ni-contact dermatitis. | ni-specific t lymphocyte clones (tlc) were isolated from two patients with ni-contact dermatitis. all of the isolated tlc required both histocompatible antigen-presenting cells (apc) and ni for induction of proliferation. by using a panel of hla-typed epstein barr virus-transformed b cells (ebv-b cells) as apc and monoclonal anti-dr antibody, the clones were shown to recognize ni in the context of hla class ii determinants. all of the clones that were isolated are okt3+, okt4+, okt8-. in the pre ... | 1985 | 2415590 |
| production of monoclonal antibodies against prostatic acid phosphatase by in vitro immunization of human spleen cells. | monoclonal antibodies against human prostatic acid phosphatase (papase) were produced by immunization of human primary spleen cell cultures. dissociated spleen cells were cultured for 5-8 days in the presence of 100 ng/ml of papase and pokeweed mitogen (1:5000). following immunization, b cells were isolated and infected with epstein-barr virus (ebv). two weeks after ebv-transformation, cells were fused with either mouse myeloma cells (sp2/oag14) or human/mouse heteromyeloma cells (shm-d33). hybr ... | 1985 | 2415632 |
| biochemical characterization of two epstein-barr virus early antigen-associated phosphopolypeptides. | epstein-barr virus (ebv) nonproducer cells nc37 induced to viral early antigen (ea) synthesis by the tumor promotor 12-o-tetradecanoylphorbol-13-acetate (tpa) were labeled at day 4 after induction with 32p, and were analyzed by immunoprecipitation with human ea-positive sera. by employing this method the appearance of two phosphopolypeptides of 50 and 58k (pp50 and pp58) was well correlated with ea complex. partial v8 protease digestion and two-dimensional peptide analysis revealed that the poly ... | 1985 | 2416113 |
| molecular analysis of the rna and protein components recognized by anti-la(ss-b) autoantibodies. | the aim of this study was to determine whether sera with autoantibodies to the la(ss-b) nuclear antigen react with the same or different sets of cellular or viral ribonucleoproteins (rnps) and whether patients with anti-la(ss-b) comprised a homogeneous group with respect to phenotypic and serological markers. the 34 anti-la(ss-b) sera studied were detected in the course of screening 2,000 sera referred from patients with suspected or defined multisystem autoimmune disease. analysis of the molecu ... | 1985 | 2417765 |
| the epstein-barr virus determined nuclear antigen is composed of at least three different antigens. | the ebv-determined nuclear antigen, ebna, is the only known viral product to be regularly detected in all ebv-transformed cells. the anticomplement immunofluorescence (acif) staining detects an ebv-specific nuclear reaction that has recently been shown to be due to at least 2 different proteins, ebna-1 and ebna-2, encoded by different parts of the viral genome. we now report the existence of a third antigen of the ebna complex, designated as ebna-3. serum from a patient with chronic infectious m ... | 1986 | 2417963 |
| establishment of a hairy cell leukemia cell line carrying tac antigen and phagocytic activity with b-cell characteristics. | a hairy cell leukemia cell line designated "hair-m" was established in a suspension culture derived from the peripheral blood of an 86-year-old japanese male with a diagnosis of hairy cell leukemia. the hair-m cells were identified as having prominent hair-like cytoplasmic projections by examination with phase-contrast and scanning electron microscopy. these cells displayed ruffled membranes and stublike microvilli similar to those observed on the surfaces of cells in the peripheral blood of the ... | 1986 | 2418246 |
| in vitro production of a human hla alloantibody of restricted specificity (dqw2) via epstein-barr virus transformation. | this paper describes the in vitro production of the human alloantibody 454a.c5 with hla-dqw2 specificity produced by a stable ebv-transformed cell line. immune b cells for transformation were generated by planned immunization of a blood donor volunteer with repeated hla-immunizations over the course of 9 yr. reactivity of 454a.c5 was in exact concordance with the presence of the dqw2 antigen as defined by microcytotoxicity assay on a panel of 21 b lymphoblastoid cell lines with well-characterize ... | 1986 | 2419282 |
| clonal analysis of hla-dpw1 (sb1) associated allodeterminants: recognition of novel epitopes and evidence for quantitative variation in class ii antigen expression. | alloreactive human t-lymphocyte clones were derived from the sb1 hla-dpw1)-specific, primed lymphocyte typing cell line sb1a used in the ninth international histocompatibility workshop. the clones from two separate subclonings were analyzed for their proliferative patterns in panel experiments with cells from unrelated individuals and in family segregation analyses. while only one clone gave a perfect correlation with the dpw1 specificity, the maturity of clones recognized multiple specificities ... | 1986 | 2419289 |
| herpes simplex virus specifies two subunits of ribonucleotide reductase encoded by 3'-coterminal transcripts. | we have previously described a transcription unit located between map coordinates 0.558 and 0.595 on the herpes simplex virus type 2 strain 333 genome which encodes two mrnas of 5.0 and 1.2 kilobases that share a common 3' terminus, and we have determined the nucleotide sequence of a 38,000-dalton protein specified by the smaller rna (d. a. galloway and m. a. swain, j. virol. 49:724-730, 1984). the entire nucleotide sequence of the 140,000-dalton protein specified by a 3,432-base-pair open readi ... | 1986 | 2419588 |
| arrest of daudi cell growth by inactive influenza virus in-vitro. | we investigated the destructive capability of three influenza a strains, victoria, pr8, and their recombinant x47, against the human lymphoma cell line daudi. both victoria and x47 strains share the same envelope glycoproteins (h3n2), while pr8, the second parental strain of x47, differs in its envelope glycoproteins (hon1). the h3n2 strains and particularly the x47 recombinant were cytotoxic to daudi cells while the hon1 strain was not. to reduce the virulence of the oncolytic viruses, we inact ... | 1985 | 2419962 |
| suppressor factors generated from human mononuclear cells by means of purified myeloma proteins. | peripheral blood mononuclear cells (pbmc) from normal human donors were cultured in marbrook flasks in the presence of purified igg or iga myeloma proteins. the culture supernatants were tested for their ability to suppress pokeweed mitogen (pwm)- or epstein-barr virus (ebv)-driven ig synthesis by normal pbmc. two supernatants from pbmc cultured with igg and one from pbmc cultured with iga were tested and suppressed pwm-driven ig synthesis as measured by a reverse haemolytic plaque assay and by ... | 1986 | 2419968 |
| human b cell activation: selective sensitivity of the early stages to calcium channel-blocking drugs. | the importance of ca2+ in the early events of lymphocyte activation has been suggested by several studies. we examined the effect of calcium channel-blocking drugs (verapamil and nitrendipine) on the progression of human b cells through their activation cycle. our results show that these drugs suppress the anti-mu-induced human b cell proliferation and interfere with the early events of the b cell activation in a dose-dependent fashion. this suppression correlates with a marked decrease in anti- ... | 1986 | 2420605 |
| isoprinosine abolishes the blocking factor-mediated inhibition of lymphocyte responses to epstein-barr virus antigens and phytohemagglutinin. | acute infectious mononucleosis (im) is accompanied by measurable abnormalities of immune function, including a transient immunosuppression. the sera of patients with acute im contain an igg blocking factor which binds to t-lymphocytes and decreases their responses to antigens and mitogens. the experiments reported herein indicate that isoprinosine, an immunopotentiating agent, can reverse this inhibition of t cells by im-associated igg blocking factor. isoprinosine may be a useful tool in unders ... | 1986 | 2420730 |
| double immunoenzymatic staining for the simultaneous detection of epstein-barr virus induced antigens. | a double indirect immunoenzymatic staining was developed for the simultaneous visualization of epstein-barr virus-induced early antigens and virus capsid antigens in p3hr1 lymphoblastoid cell line. the double immunocytochemical staining was performed with a four-stage and a two-stage procedure employing human sera and monoclonal antibodies against epstein-barr virus-induced antigens, followed by the addition of specific alkaline phosphatase and peroxidase labeled antisera. the selection of subst ... | 1986 | 2420755 |
| expression of mhc class ii and tac antigens on il2-activated human t cell clones that can stimulate in mlr, amlr, plt and can present antigen. | the expression of interleukin 2 (il2) receptor and hla-dr, dq, and dp antigens on the surface of four diphtheria toxoïd (dt)-specific t lymphocyte clones (tlc) and two tlc specific for an allogeneic ebv-transformed cell line was investigated with the use of monoclonal antibodies (moabs) that recognize defined molecules or epitopes. incubation of a resting tlc with il2 resulted in a 10- to 30-fold increase in the level of dr, dq, and tac antigen expression. on the other hand, incubation of an act ... | 1986 | 2420767 |
| a monoclonal antibody against hla-a11 and a24. | a monoclonal igg antibody was produced from a mouse immunized with an a11, a24; b27, b44 epstein-barr virus transformed b lymphoblastoid cell line. the antibody, a11.1m, by standard lymphocytotoxicity assay, reacts with all cells expressing hla-a11 and -a24. absorption studies with both a11+, a24- and a11-, a24+ platelets removed antibody reactivity against a11 and a24 lymphocytes. the shared antigenic determinant between a11 and a24, as defined by this antibody, a11.1m, represents a new "supert ... | 1986 | 2420768 |
| interferon and 2-5a synthetase in patients with immune diseases. | | 1985 | 2421319 |
| a chromosomal breakage syndrome with profound immunodeficiency. | the chromosomal breakage syndromes--ataxia-telangiectasia, fanconi's anemia, and bloom's syndrome--are associated with growth failure, neurologic abnormalities, immunodeficiency, and an increased incidence of malignancy. the relationship between these features is unknown. we recently evaluated a 21-year-old female with more severe chromosomal breakage, immunodeficiency, and growth failure than in any of the mentioned disorders. as of november 1985, the patient remains clinically free of malignan ... | 1986 | 2421804 |
| epstein-barr virus-transformed lymphocytes from patients with bullous diseases produce autoantibodies to cytokeratins. | human cell lines obtained after epstein-barr virus transformation of lymphocytes from seven patients with bullous diseases (bullous pemphigoid, pemphigus) and five controls were screened for the production of autoantibodies against skin antigens. in five out of seven patients, the culture supernatants tested by indirect immunofluorescence on frozen sections of normal human skin and rabbit lip showed the production of autoantibodies with different specificities: basal epidermal cells, whole epide ... | 1986 | 2421952 |
| indirect alkaline phosphatase immunoenzymatic staining for the detection of antibodies to epstein-barr virus-induced virus capsid antigens and early antigens. | an indirect alkaline phosphatase immunoenzymatic staining technique was developed for the detection of antibodies against epstein-barr virus-induced virus capsid antigens and early antigens in cell smears. the presence of antibodies against epstein-barr virus-induced virus capsid antigens and early antigens was revealed by a dark blue staining of cells expressing the antigens. the alkaline phosphatase assay gave a permanent record of the reaction that could be visualized under an ordinary light ... | 1986 | 2422201 |
| rheumatoid arthritis synovial membrane contains a 62,000-molecular-weight protein that shares an antigenic epitope with the epstein-barr virus-encoded associated nuclear antigen. | a monoclonal antibody, selected for reactivity with the epstein-barr virus (ebv)-encoded antigen ebna-1, exhibited strong reactivity with the synovial lining cells in joint biopsies from 10 of 12 patients with rheumatoid arthritis (ra) and adherent cells eluted from these tissues. no staining of ra synovial membrane frozen tissue sections or eluted synovial-lining cells was obtained with monoclonal antibodies directed against other ebv-encoded antigens (anti-p160, anti-gp200/350) or with monoclo ... | 1986 | 2422209 |
| an influenza a virus-specific and hla-drw8-restricted t cell clone cross-reacting with a transcomplementation product of the hla-dr2 and dr4 haplotypes. | the clone ta10 is a t3+ t4+ t8- proliferative and cytolytic human t cell clone. this clone has been shown to be specific for the hemagglutinin of influenza a texas virus and restricted by an hla class ii molecule associated with the drw8-dw8.1 phenotype. here we show that ta10 and all of its subclones can also react with eight hla-drw8 negative, epstein-barr virus (ebv)-transformed cell lines or phytohemagglutinin blasts in the absence of influenza antigens. all of these cell lines are hla-dr2/d ... | 1986 | 2422266 |
| purification of the epstein-barr virus/c3d complement receptor of human b lymphocytes: antigenic and functional properties of the purified protein. | the epstein-barr virus/c3d receptor (cr2) of human b lymphocytes was purified to homogeneity from raji cells by immunoaffinity chromatography. the average yield of the 145-kilodalton receptor was 400 pmol (50 micrograms) per 10(10) cells, representing an approximate 75% recovery. the isolated 145-kilodalton protein was antigenically and functionally intact as it reacted with several anti-cr2 monoclonal antibodies and bound purified epstein-barr virus and c3d,g. these findings with the purified m ... | 1986 | 2422399 |
| characterization of the restricted component of epstein-barr virus early antigens as a cytoplasmic filamentous protein. | four monoclonal antibodies produced against the restricted component of the epstein-barr virus (ebv) early antigen (ea-r) precipitated a polypeptide with an approximate molecular weight of 85,000. three of these antibodies prepared against the native 85,000-molecular-weight protein (85k protein) reacted by immunofluorescence with acetone-fixed smears but not methanol-fixed smears of ebv-producing cells activated with tumor-promoting agent and sodium butyrate. the fourth monoclonal antibody which ... | 1986 | 2422401 |
| human monoclonal antibody directed against an envelope glycoprotein of human t-cell leukemia virus type i. | we report the production and characterization of a human monoclonal antibody reactive against the major envelope glycoprotein of human t-cell leukemia virus type i (htlv-i), a virus linked to the etiology of adult t-cell leukemia. we exposed lymph-node cells derived from a patient with adult t-cell leukemia to the epstein-barr virus in vitro and obtained a b-cell clone (designated 0.5 alpha) by a limiting dilution technique. the secreted product of 0.5 alpha is a monoclonal antibody (also design ... | 1986 | 2422659 |
| hla class-ii-restricted mycobacterium leprae-reactive t-cell clones from leprosy patients established with a minimal requirement for autologous mononuclear cells. | this report describes an effective method for the cloning of mycobacterium leprae-reactive t lymphocytes with epstein-barr-virus transformed autologous b cells as antigen-presenting cells. the two advantages of this method are that it drastically reduces the number of autologous peripheral blood mononuclear cells (less than 10(7) cells) needed to obtain and propagate these t-cell clones (tlc), and that it enables us to expand individual tlc to large numbers of cells (greater than 10(8)). thus th ... | 1986 | 2422737 |
| establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. | using a serum-free defined medium, we have established a human cell line, nci-h929, from a malignant effusion occurring in a patient with igak myeloma. the cultured cells have the morphologic, ultrastructural, biochemical, immunologic, and cytochemical features of plasma cells. the cells have rearranged alpha and kappa genes and synthesize and secrete high amounts of igak (greater than 80 micrograms/10(6) cells per 24 hours). the cells express surface immunoglobulin (alpha and kappa), the plasma ... | 1986 | 2423157 |
| a new supertypic hla class ii determinant (pl2) differentially expressed with dr7, drw11(5), drw13(w6), drw14(w6), dr3, and dr2 and biochemically localized to dr7 molecules. | a monoclonal antibody, pl2, has been produced that reacts with a new supertypic determinant expressed on the peripheral blood b lymphocytes and b-leukemic cells (b-cll) from all individuals who are hla-dr7 and some individuals who are hla-dr5 positive. the genetic linkage of the pl2 determinant to the hla region was demonstrated by family segregation studies. when cultured epstein-barr virus (ebv) transformed b cell lines were examined, pl2 was again found to be expressed on all cell lines homoz ... | 1986 | 2423484 |
| establishment of a human b cell line that proliferates in response to b cell growth factor. | a human b cell line which shows a marked dose dependence on b cell growth factor (bcgf) when cultured in less than or equal to 2% serum has been established. human b lymphocytes were obtained from peripheral blood of normal donors and cultured in the presence of anti-igm (mu chain specific) and bcgf. frequent refeedings with fresh medium containing bcgf and anti-igm led to the establishment of a long term cultured human b cell line, hab-40. phenotyping of hab-40 revealed that the cell population ... | 1986 | 2423613 |
| monoclonal antibodies against su-dhl-1 cells stain the neoplastic cells in true histiocytic lymphoma, malignant histiocytosis, and hodgkin's disease. | three murine monoclonal antibodies, named 2h9, 1e9 and 1a2, were produced after immunization of balb/c mice with cells of the su-dhl-1 cell line from a true histiocytic lymphoma. in frozen sections from various lymphomas, 2h9 and 1a2 selectively stained the cell membranes of neoplastic cells in true histiocytic lymphoma and hodgkin's disease. antibody 1e9 stained the nuclear membranes of the tumor cells in true histiocytic lymphoma and malignant histiocytosis. no staining was seen in 56 cases of ... | 1986 | 2424524 |
| [circulating igg-containing immune complexes in malignant growth]. | the data from literature on detection and characteristics of circulating immune complexes in malignancy are analyzed. special attention is paid to determination of immune complex components which contain tumour-associated antigens and anti-tumour antibodies. prospects of application of the identified components of immune complexes in immune diagnostics and immune therapy of malignancy are discussed. | 1986 | 2424714 |
| demonstration of epstein-barr virus in malignant non-hodgkin's lymphomas. | lymph nodes or tumor biopsies of 60 persons suspected of having a malignant lymphoma were examined for the presence of epstein-barr virus nuclear antigen (ebna) by anticomplement immunofluorescence. in 8 cases the tissue specimens were also assayed for epstein-barr virus (ebv) dna by nucleic acid hybridization. serum samples of patients and controls were tested for ebv-related antibodies. the histological tests in 37 cases showed a malignant non-hodgkin lymphoma, and in 23 cases a reactive lymph ... | 1986 | 2425318 |
| antiviral therapy. new drugs and their uses. | | 1986 | 2425347 |
| functional analysis of the hla-a2/aw68 recombinant molecule hla-aw69: epstein-barr virus specific cytotoxic t cells restricted through aw69 can utilise restricting determinants on aw68 but not on a2. | epstein-barr virus-specific cytotoxic t cell lines, expanded in interleukin 2-conditioned medium, have been generated in vitro from virus-immune individuals bearing either the common hla-a2 antigen (a2.1), a variant a2 antigen (a2.2) or a novel antigen aw69 which is a recombinant molecule having identity with hla-aw68 in the alpha 1 domain and with hla-a2 in the alpha 2 and alpha 3 domains. virus-specific cytotoxic t cells restricted through a2.1 or a2.2 only recognised targets bearing the ident ... | 1986 | 2425456 |
| expression of the epstein-barr virus 138-kda early protein in escherichia coli for the use as antigen in diagnostic tests. | we have attempted to produce the 138-kda early protein (ep 138) of epstein-barr virus (ebv) in escherichia coli. this protein was found, by immunoprecipitation, to be a clinically relevant antigen, especially for the determination of the iga-titer in patients with nasopharyngeal carcinoma (npc). since the expression of the entire ep 138 coding region was unsuccessful, we synthesized only the antigenic parts of this protein. potential antigenic sites were predicted from the amino acid sequence by ... | 1986 | 2426158 |
| control of human b-lymphocyte replication. ii. transforming epstein-barr virus exploits three distinct viral signals to undermine three separate control points in b-cell growth. | highly purified resting (go) b lymphocytes were monitored for their response to transforming epstein-barr virus (b95-8 strain), to a non-transforming mutant (p3hr-1) containing a deletion in the ebna-2 coding region, and to inactivated virus of either type. all preparations induced an early appearance of two activation antigens, which included the cd23,p45 ("blast-2') antigen. thus, virus binding was sufficient for an initial activation step. further change required an active viral genome. infec ... | 1986 | 2426189 |
| expression of the epstein-barr virus nuclear protein 2 in rodent cells. | a 3.0-kilobase-pair epstein-barr virus (ebv) dna segment necessary for lymphocyte immortalization encodes at least part of a nuclear protein (ebna2) which is characteristically expressed in latently infected, immortalized cells. a 1.5-kilobase open reading frame within this dna segment has now been inserted into a murine leukemia virus (mulv)-derived expression vector (pzip-neo-sv(x)1) which provides for transcription of heterologous dna but not for translational start. transfection of the recom ... | 1986 | 2426468 |
| natural killer susceptibility of human cells may be regulated by genes in the hla region on chromosome 6. | natural killer (nk) cells exist in each individual in the absence of any intentional immunization. they are able to kill a wide range of targets from tumoral as well as from normal origin. however, their exact physiologic role is not clearly understood. in this study we report results about a human epstein-barr virus-transformed b-cell line from which variants perturbed in the expression of hla molecules have been derived. our results indicate that in these cell lines an inverse relationship exi ... | 1986 | 2426704 |
| igg anti-tetanus toxoid antibody production induced by epstein-barr virus from b cells of human marrow transplant recipients. | this investigation shows that epstein-barr virus (ebv)-activated human b cells from marrow transplant recipients can produce in vitro igg anti-tetanus toxoid antibody (anti-tt) without booster immunizations with tetanus toxoid (tt). purified b cells (e-rosette negative) from 8 normal subjects, 6 healthy long-term marrow graft recipients, and 15 long-term marrow graft recipients with chronic graft-vs-host disease (gvhd), were stimulated for 12 days with ebv to induce anti-tt production in culture ... | 1986 | 2427210 |
| polymyositis and molecular mimicry, a mechanism of autoimmunity. | the amino acid sequences of escherichia coli histidyl-trna synthetase and alanyl-trna synthetase, two proteins recently identified as autoantigens in polymyositis, were compared by a computer alignment procedure with those of the 3600 proteins tabulated in the national biomedical research foundation protein sequence database. both proteins contain sequences long enough to function as epitopes that match sequences on viral and muscle proteins. the homology thus revealed not only lends strong supp ... | 1986 | 2427902 |
| monoclonal antibodies to hla-dp, dq, and dr determinants: functional effects on the activation and proliferation of normal and ebv-transformed b cells. | to investigate the function of hla class ii molecules in b-cell activation, we generated three new anti-hla class ii monoclonal antibodies with differing specificities for dp, dq, and dr determinants. these were tested for their ability to inhibit various b- and t-lymphocyte responses. each of these antibodies (nb-29, dh-84, and dh-224) immunoprecipitates a heterodimer of approximately 35,000 and 28,000 mol wt from 125i-surface-labeled b-lymphoma cells, as shown by sds-page. nb-29 (igg1) detects ... | 1986 | 2428652 |
| t cell clones which share t cell receptor epitopes differ in phenotype, function and specificity. | recently, we described a monoclonal antibody (3d6) that reacts with the t cell receptor (ti) of the t leukemic cell line hpb-all and that cross-reacts with 2-10% of the t cells of normal healthy individuals. in this study we report the establishment of t cell clones that are 3d6+ but that differ in function and phenotype. these clones were established according to two different protocols: t cells of donor hy (10% 3d6+) were stimulated with the epstein-barr virus-transformed cell line jy. the pro ... | 1986 | 2429845 |
| analogous amino acid sequences in myelin proteolipid and viral proteins. | computer analysis of the intrinsic membrane protein, myelin proteolipid, shows strong sequence similarities between the putative extramembrane segments of the proteolipid protein and a number of viral proteins, several of which infect humans. these similarities are even more striking than those reported previously between viral proteins and the encephalitogenic myelin basic protein (mbp). these findings, along with other reports of molecular mimicry by viruses, suggest that immunological cross-r ... | 1986 | 2429866 |
| regulation of virus infections by natural killer cells. a review. | | 1986 | 2430177 |
| induction of oligo-2',5'-adenylate synthetase in human lymphoid cells treated with 5-azacytosine and 5-iododeoxyuridine. | oligo-2',5'-adenylate synthetase activity was investigated in several human lymphoblastoid cell lines of b cell origin treated with reagents having effects on gene expression, 5-azacytosine (5azct), 5-azacytidine (5azcd) and 5-iodo-2'-deoxyuridine (iudr). enzyme activity did not increase in cells treated with 5azcd, but the other reagents induced significant levels of activity. interferon (ifn) activity was not detected in culture fluids of cells treated with 5azcd or 5azct. on the other hand, t ... | 1986 | 2431103 |
| a human monoclonal antibody to an hla-drw53 (mt3)-like epitope on class ii antigens. | we report the production of a lymphoblastoid human b cell line, which secretes monoclonal antibody with hla-drw53-like specificity. the immune lymphocytes were obtained from a patient who had been undergoing immunotherapy at memorial hospital with allogeneic cultured melanoma cells. the antibody producing cell line was established by transformation of immune b cells with epstein-barr virus and stabilized by serial selection of antibody-positive cell clusters. the cell line (r39.14) has been prod ... | 1986 | 2431513 |
| the glycine-alanine repeating region is the major epitope of the epstein-barr nuclear antigen-1 (ebna-1). | the epstein-barr nuclear antigen-1 (ebna-1) is a protein containing a large glycine-alanine repeat that has been shown to be antigenic. antibodies to ebna-1 can be detected by means of immunoblotting. preincubation of antisera with purified ebna-1 protein inhibits the binding of igg antibodies in this system, indicating that those epitopes detected by immunoblots are also accessible on the native molecule. a number of synthetic peptides the sequences of which were derived from the glycine-alanin ... | 1987 | 2432130 |
| dna synthesis inhibitors suppress expression of epstein-barr virus in chemically activated human lymphoblastoid cells. | of the dna synthesis inhibitors tested, novobiocin, nalidixic acid, coumermycin a1, mitomycin c, hydroxyurea, and bleomycin effectively inhibited epstein-barr virus (ebv)-related early antigen (ea) synthesis induced in human lymphoblastoid cells by 12-o-tetradecanoyl-phorbol-13-acetate and n-butyrate, but did not inhibit ea synthesis in 12-o-tetradecanoyl-phorbol-13-acetate and n-butyrate, but did not inhibit ea synthesis in cells superinfected with ebv. inhibition of ea synthesis by treatment w ... | 1986 | 2433251 |
| the isolation and sequence of a novel gene from a human functional t cell line. | using a subtractive hybridization procedure we have constructed a cdna library enriched for sequences present in functional human t cell lines, but not in human ebv-transformed b cell lines. we have isolated a cdna clone, ah2-519, representing a novel gene, designated 519. this novel gene is expressed in functional human cytolytic and th cell lines but not in a variety of other cell lines, including several long-term human t cell tumor lines. the expression of gene 519 is inducible in cultures o ... | 1987 | 2434598 |
| epitope mapping of the major epstein-barr virus outer envelope glycoprotein gp350/220. | to understand the complete immunochemical structure of the epstein-barr virus (ebv) major membrane glycoprotein gp350/220, monoclonal antibodies (mabs) reacting with this important viral antigen were isolated. through competitive inhibition binding studies, it was determined that a group of 18 igg mabs recognized seven distinct epitopes on the gp350/220 molecule. eight of these mabs fell into a single epitope group with four of those mabs, as well as a single mab from another epitope group, bein ... | 1987 | 2434608 |
| treatment of virus-associated tumors with interferon. | | 1986 | 2434902 |
| prevention and treatment of epstein-barr virus (ebv)-associated lymphoproliferative diseases in immune deficient patients. | prevention of ebv-associated lymphoproliferative diseases in immune deficient individuals is preferred; however, standard therapy for the b cell lymphomas has been successful. chemotherapy must be given cautiously lest further immune compromise result in opportunistic infections. recently, acyclovir has decreased morbidity of patients with acute infectious mononucleosis in immune competent persons. in contrast, immunodeficient patients with x-linked lymphoproliferative (xlp) syndrome do not seem ... | 1986 | 2435295 |
| gamma globulin therapy for chronic mononucleosis syndrome. | antibodies against epstein-barr virus, associated with antibody dependent cytotoxic cell activity, were found to be present in diminished titer in 20 of 22 patients tested with chronic mononucleosis syndrome (cms). gamma globulin was shown to improve symptoms in 53% of the patients treated, compared with 32% of placebo injections. 89.5% of 57 patients treated with a gamma globulin treatment program remained in the treatment program because of relief of symptoms, and only four patients dropped ou ... | 1986 | 2435296 |
| genotoxicity of diesel exhaust emissions in a battery of in-vitro short-term and in-vivo bioassays. | the present studies confirm that diesel exhaust emissions are mutagenic in bacterial and mammalian mutation assays. our results further indicate that mutagenic potential of the diesel tar samples can be reduced by exogenous metabolic activation with s15, and that epstein-barr-virus transformed lymphoblastoid cell lines from bloom syndrome, and xeroderma pigmentosum patients with a high incidence of malignant tumors showed an larger production of sces while those from fanconi anemia patients had ... | 1986 | 2435508 |
| autocrine growth function of human interleukin 1 molecules on roha-9, an ebv-transformed human b cell line. | in this study we report that the roha-9 cell line, an il 1-secreting ebv-transformed human b cell line, exhibits an autocrine pathway of growth. in fact, roha-9 cells spontaneously secreted an autoregulatory growth factor that co-purified with the constitutively secreted il 1-like molecules. accordingly, monocyte-derived human il 1, free of other known biological activities, also stimulated the growth of roha-9 cells in a dose-dependent way. human recombinant interleukin 2, recombinant ifn-alpha ... | 1987 | 2435800 |
| immune response to intermediate filament-associated, epstein-barr virus-induced early antigen. | a murine monoclonal antibody (mab) (15td3) was found to recognize a unique filamentous structure in epstein-barr virus (ebv)-producing lymphoblastoid cell lines. by immunofluorescent morphology, in comparison with a control mab to vimentin, the 15td3 filamentous structure was judged to be associated with intermediate filaments of the cytoskeleton. expression of the 15td3 antigen and vimentin was induced simultaneously in some ebv genome-positive cell lines either by ebv superinfection or by 12-0 ... | 1987 | 2435803 |
| autoantibodies in infectious mononucleosis have specificity for the glycine-alanine repeating region of the epstein-barr virus nuclear antigen. | viruses have been postulated to be involved in the induction of autoantibodies by: autoimmunization with tissue proteins released by virally induced tissue damage; immunization with virally encoded antigens bearing molecular similarities to normal tissue proteins; or nonspecific (polyclonal) b cell stimulation by the infection. infectious mononucleosis (im) is an experiment of nature that provides the opportunity for examining these possibilities. we show here that igm antibodies produced in thi ... | 1987 | 2435830 |
| antiviral therapy. | | 1987 | 2436295 |
| human monoclonal antibodies that recognize conserved epitopes in the core-lipid a region of lipopolysaccharides. | epstein-barr virus (ebv)-transformed human b lymphocytes were fused with a murine-human heteromyeloma to produce stable hybrid cell lines that secreted human monoclonal antibodies (mabs) of the igm class that recognized conserved epitopes in the core-lipid a region of lipopolysaccharides (lps). three of the mabs reacted with epitopes on the lipid a moiety, while a fourth recognized a determinant in the core oligosaccharide. the lipid a-specific mabs cross-reacted with heterologous rough lps and ... | 1987 | 2437155 |
| monoclonal antibodies to the latent membrane protein of epstein-barr virus reveal heterogeneity of the protein and inducible expression in virus-transformed cells. | monoclonal antibodies specific for the 'latent membrane protein' (lmp) of epstein-barr virus (ebv), one of the effector proteins of ebv-induced b cell transformation, have been generated from mice immunized with a beta-galactosidase fusion protein containing the carboxyl half of the b95.8 strain lmp sequence. four monoclonal igg1 antibodies, designated cs.1, cs.2, cs.3 and cs.4, which together recognized at least three different epitopes on the molecule, were used to examine various aspects of l ... | 1987 | 2438376 |
| nuclear dna-binding proteins determined by the epstein-barr virus-related simian lymphotropic herpesviruses h. gorilla, h. pan, h. pongo and h. papio. | nuclear dna-binding proteins were extracted from lymphoblastoid cell lines transformed with epstein-barr virus (ebv) or with the related lymphotropic herpesviruses of gorilla (herpesvirus gorilla), chimpanzee (h. pan), baboon (h. papio) or orang-utan (h. pongo). they were immunoblotted with the sera of all four simian species in comparison with ebv antibody-positive human sera. eight nuclear proteins were identified, and were designated gona-1 and gona-2 for h. gorilla-determined nuclear antigen ... | 1987 | 2438377 |
| release of lymphokines following epstein-barr virus infection in vitro of blood lymphocytes from patients with autoimmune diseases. | | 1986 | 2440173 |
| multiple hla class i-dependent cytotoxicities constitute the "non-hla-restricted" response in infectious mononucleosis. | primary epstein-barr virus (ebv) infection, when manifest as infectious mononucleosis (im), induces a broad-ranging and apparently non-hla-restricted cytotoxic response whose nature has not been resolved. in the present experiments the ability to cryo-preserve im mononuclear cell preparations, after depletion of cd16+ natural killer cells, has allowed detailed analysis of the response on appropriately constructed target cell panels. the results show that im effector preparations are polyclonal w ... | 1987 | 2440688 |
| analytical studies of the binding parameters describing the interaction of hla-dr epitopes with a specific monomorphic monoclonal antibody. | in this paper we present an analytical study of the binding parameters related to the interactions of the hla-dr-specific monoclonal antibody (l243) and its reacting epitope as expressed on the cell surface of seven epstein-barr virus-transformed b cell lines. scatchard, sips and langmuir equations were used to plot and analyse the data obtained from each reaction. a single affinity constant (k) value was derived at low and high concentrations of free antibody for each antibody-cell interaction ... | 1987 | 2440953 |
| characterization of a cdna clone corresponding to a transcript from the epstein-barr virus bamhi m fragment: evidence for overlapping mrnas. | a 1.95-kilobase cdna clone was isolated by screening a size-selected lambda gt10 cdna library prepared from an epstein-barr virus-transformed b-cell line, ib4, with the epstein-barr virus bamhi m fragment. sequence analysis revealed that this clone contains about 75% of the bmrf1 and the complete bmrf2 open reading frames. the transcript is not spliced, and the polyadenylation signal at base pair 2641 of the bamhi m fragment is used. northern blots (rna blots) indicate that this polyadenylation ... | 1987 | 2441081 |
| a human monoclonal antibody against hla-a25. | we are reporting the production and characterization of a human monoclonal antibody recognizing antigen hla-25. the antibody was developed by a line transformed in vitro by the epstein-barr virus. the immune b lymphocytes for transformation were generated by planned immunization of a volunteer with repeated doses of allogenic peripheral blood lymphocytes of one donor over the course of 7 years. the antibody showed correlation with a25 antigen on a panel of 244 individuals tested by microcytotoxi ... | 1987 | 2441487 |
| a human monoclonal antibody that recognizes viral polypeptides and in vitro translation products of the genome of the hepatitis d virus. | peripheral blood lymphocytes from a patient chronically infected with hepatitis d virus (hdv) were immortalized by epstein-barr virus transformation. two stable monoclonal cell lines, derived from the same parent culture, were established and produced antibodies of the igg isotype that were specific for the hepatitis delta antigen (hdag). both monoclonal antibodies (mabs) recognized the major hdag polypeptides of 24 kilodaltons and 27 kilodaltons that were previously detected by polyclonal antib ... | 1987 | 2442271 |
| phorbol ester and epstein-barr virus dependent transformation of normal primary human skin epithelial cells. | substantial evidence has implicated epstein-barr virus (ebv) in the aetiology of two human neoplasms, nasopharyngeal carcinoma (npc) and burkitt's lymphoma. this is supported by the presence of high antibody titres to ebv early antigen and virus capsid antigen, as well as antibody to two viral-associated enzymes, dnase and dna polymerase. patients with npc, particularly the undifferentiated form, are commonly found to have ebv dna in the tumour. ito and others have presented strong epidemiologic ... | 1987 | 2442617 |