| antibacterial activities of novel nicotinic acid hydrazides and their conversion into n-acetyl-1,3,4-oxadiazoles. | synthesis of a series of novel n-acylhydrazones of nicotinic acid hydrazides 3a-j via condensation of nicotinic acid hydrazide 1 with the corresponding aldehydes and ketones is described. the series 3a-j was evaluated for in vitro antibacterial activity against two gram negative (pseudomonas aeruginosa and klebsiella pneumoniae) and two gram positive (streptococcus pneumoniae and staphylococcus aureus) bacteria. the zone of inhibition was measured using the disk diffusion method, and in vitro mi ... | 2014 | 25454271 |
| characterization of resistance genes in 68 esbl-producing klebsiella pneumonia in lebanon. | we studied 68 esbl-producing klebsiella pneumoniae strains isolated in lebanon, determined their profile of resistance to antibiotics, and identified 6 esbl genes. | 2014 | 25453362 |
| evaluation of a simple phenotypic method for the detection of carbapenemase-producing enterobacteriaceae. | we investigated the performance of a phenotypic test, the carbapenemase detection set (mast-cds), for the identification of carbapenemase-producing enterobacteriaceae. our results indicated that mast-cds is rapid, easily performed, simple to interpret, and highly sensitive for the identification of carbapenemase producers, particularly imipenemase producers. | 2015 | 25451461 |
| identification of capsular types in carbapenem-resistant klebsiella pneumoniae strains by wzc sequencing and implications for capsule depolymerase treatment. | klebsiella pneumoniae is an important human pathogen associated with a variety of diseases, and the prevalence of multidrug-resistant k. pneumoniae (mdrkp) is rapidly increasing. here we determined the capsular types of 85 carbapenem-resistant k. pneumoniae (crkp) strains by wzc sequencing and investigated the presence of carbapenemases and integrons among crkp strains. ten crkp strains (12%) were positive for carbapenemase (imipenemase, 6/85 strains; k. pneumoniae carbapenemase, 3/85 strains; v ... | 2015 | 25451047 |
| dissemination of a clone carrying a fosa3-harbouring plasmid mediates high fosfomycin resistance rate of kpc-producing klebsiella pneumoniae in china. | fosfomycin has been proposed as an adjunct to other active agents for treating kpc-producing klebsiella pneumoniae infections. this study aimed to investigate the prevalence of fosfomycin resistance and plasmid-mediated resistance determinants among kpc-producing k. pneumoniae isolates from clinical samples in china. in total, 278 kpc-producing and 80 extended-spectrum β-lactamase (esbl)-producing (non-kpc-producing) clinical k. pneumoniae isolates were collected in 12 hospitals from 2010 to 201 ... | 2015 | 25450805 |
| characteristics, risk factors and outcomes of carbapenem-resistant klebsiella pneumoniae infections in the intensive care unit. | to study the characteristics, risk factors and outcomes of intensive care unit (icu) patients with carbapenem-resistant (crkp) and carbapenem-susceptible (cskp) klebsiella pneumoniae infections. | 2015 | 25447713 |
| characterisation of the first ongoing outbreak due to kpc-3-producing klebsiella pneumoniae (st512) in spain. | the aim of this study was to characterise carbapenem-resistant klebsiella pneumoniae isolates that caused an outbreak in a hospital in the south of spain, originating from a patient transferred in 2012 from italy. forty-four k. pneumoniae isolates, recovered from 28 patients, were screened by pcr for extended-spectrum β-lactamase (esbl) and carbapenemase genes and the products were further sequenced. plasmids were transferred by electroporation and were classified using pcr-based inc/rep typing ... | 2014 | 25446907 |
| regulator of the mucoid phenotype a gene increases the virulent ability of extended-spectrum beta-lactamase-producing serotype non-k1/k2 klebsiella pneumonia. | to determine whether the presence of a capsule regulator gene [i.e., regulator of mucoid phenotype a (rmpa) gene] contributes to virulence on extended-spectrum β-lactamase-producing klebsiella pneumoniae (esbl-kp) with serotype non-k1/k2 strains. | 2016 | 25442871 |
| impact of revised susceptibility breakpoints on bacteremia of klebsiella pneumoniae: minimum inhibitory concentration of cefazolin and clinical outcomes. | the clinical and laboratory standards institute (clsi) revised the susceptibility breakpoints of cephalosporins for enterobacteriaceae in 2010 and 2011. however, there is a lack of clinical data about the correlation of minimum inhibitory concentrations (mics) and clinical outcome. data for the distribution of mics and clinical outcomes were analyzed in this study to evaluate the impact of changes in the clsi breakpoints on the treatment of klebsiella pneumoniae bacteremia. | 2016 | 25442869 |
| identification of the first imported kpc-3 klebsiella pneumoniae from the usa to taiwan. | establishment of kpc-associated genes into a new region usually requires travellers with hospital admission and their carriage into the communities. in this report, a worldwide spreading clone carrying kpc-3 was isolated from the sputum of a hospitalised patient with a serious infection who had just come from the usa and had been admitted to a new york hospital. by genetic comparison with a strain isolated from new jersey (nj-kpc-21), this isolate from the traveller was genetically related. the ... | 2014 | 25442359 |
| exploring dangerous connections between klebsiella pneumoniae biofilms and healthcare-associated infections. | healthcare-associated infections (hai) are a huge public health concern, particularly when the etiological agents are multidrug resistant. the ability of bacteria to develop biofilm is a helpful skill, both to persist within hospital units and to increase antibiotic resistance. although the links between antibiotic resistance, biofilms assembly and hai are consensual, little is known about biofilms. here, electron microscopy was adopted as a tool to investigate biofilm structures associated with ... | 2014 | 25438020 |
| a dimeric chlorite dismutase exhibits o2-generating activity and acts as a chlorite antioxidant in klebsiella pneumoniae mgh 78578. | chlorite dismutases (clds) convert chlorite to o2 and cl(-), stabilizing heme in the presence of strong oxidants and forming the o═o bond with high efficiency. the enzyme from the pathogen klebsiella pneumoniae (kpcld) represents a subfamily of clds that share most of their active site structure with efficient o2-producing clds, even though they have a truncated monomeric structure, exist as a dimer rather than a pentamer, and come from gram-negative bacteria without a known need to degrade chlo ... | 2015 | 25437493 |
| first cases of kpc-type carbapenemase-producing bacteria in patients in new zealand hospitals. | the emergence and global spread of klebsiella pneumoniae carbapenemases (kpcs) is a significant public health problem. between october 2010 and july 2013, kpc-producing k. pneumoniae were isolated from four patients in new zealand hospitals. these cases are the first known isolations of kpc-producing organisms in new zealand. all four patients were transferred from, or had recently been in, hospitals in countries where kpc-producing organisms are prevalent (china, india, greece and italy). the b ... | 2014 | 27873697 |
| emergence of plasmid mediated carbapenemase oxa-48 in a klebsiella pneumoniae strain in algeria. | a carbapenem resistant klebsiella pneumoniae strain was isolated in october 2011 in the pediatric unit of the hôpital central de l'armée in algeria, this strain have been confirmed for the production of oxa-48 carbapenemase. the blaoxa-48 gene was located on a self conjugative plasmid of 70kb. multilocus sequence typing indicated the presence of the sequence type st-307. this is the first isolation of oxa-48-producing k. pneumoniae in algeria. | 2014 | 27873696 |
| multi-drug resistant gram negative infections and use of intravenous polymyxin b in critically ill children of developing country: retrospective cohort study. | patients in pediatric intensive care units (picu) are susceptible to infections with antibiotic resistant organisms which increase the morbidity, mortality and cost of care. to describe the clinical characteristics and mortality in patients with multi-drug resistant (mdr) gram negative organisms. we also report safety of polymyxin b use in these patients. | 2014 | 25430979 |
| genome sequence of a multidrug-resistant strain of klebsiella pneumoniae, bamc 07-18, isolated from a combat injury wound. | klebsiella pneumoniae is an important infectious agent of surgical sites and combat wounds. antibiotic resistance and tolerance are common impediments to the healing of chronic infections. here, we report the genome sequence of a highly multidrug-resistant strain of k. pneumoniae, bamc 07-18, isolated from a combat wound of a soldier. | 2014 | 25428975 |
| experimental evidence for is1294b-mediated transposition of the blacmy-2 cephalosporinase gene in enterobacteriaceae. | the objective of this study was to investigate whether the insertion sequence is1294b (is91 family) is able to mobilize the blacmy-2 gene and its adjacent regions from one replicon to another. | 2015 | 25428924 |
| emergence of colistin resistance in klebsiella pneumoniae from veterinary medicine. | | 2015 | 25428921 |
| klebsiella variicola is a frequent cause of bloodstream infection in the stockholm area, and associated with higher mortality compared to k. pneumoniae. | clinical isolates of klebsiella pneumoniae are divided into three phylogroups and differ in their virulence factor contents. the aim of this study was to determine an association between phylogroup, virulence factors and mortality following bloodstream infection (bsi) caused by klebsiella pneumoniae. isolates from all adult patients with bsi caused by k. pneumoniae admitted to karolinska university hospital, solna between 2007 and 2009 (n = 139) were included in the study. phylogenetic analysis ... | 2014 | 25426853 |
| antibacterial and antioxidant constituents of acalypha wilkesiana. | this study was aimed at characterising the secondary metabolites responsible for antibacterial and antioxidant activities of acalypha wilkesiana. purification of the defatted methanol leaves extract was guided by the dpph free radical scavenging assay as well as by evaluation of the antibacterial activity against four bacterial strains. as a result, geraniin, corilagin, quadrangularic acid m and shikimic acid were purified and isolated. shikimic acid, reported for the first time from this plant, ... | 2015 | 25426700 |
| carbapenem resistant gram-negative bacteremia in an indian intensive care unit: a review of the clinical profile and treatment outcome of 50 patients. | growing antimicrobial resistance and limited therapeutic options to treat carbapenem-resistant bacteremia prompted us to evaluate the clinical outcomes associated with healthcare-associated bacteremia. | 2014 | 25425843 |
| in vitro antibacterial phenolic extracts from "sugarbag" pot-honeys of australian stingless bees (tetragonula carbonaria). | australian stingless bee honeys have been shown to exert antioxidant and in vitro antimicrobial properties; however their bioactive factors remained unidentified. this study investigated the antibacterial properties of phenolic extracts from tetragonula carbonaria honeys. honeys were harvested from beehives in three sites of south east australia. liquid-liquid extractions yielded the phenolic concentrates, for analyses by liquid and gas chromatography mass spectrometry. antibacterial assays were ... | 2014 | 25423113 |
| complete nucleotide sequences of two ndm-1-encoding plasmids from the same sequence type 11 klebsiella pneumoniae strain. | the sequence type 11 klebsiella pneumoniae strain kpn-3002cz was confirmed to harbor two ndm-1-encoding plasmids, pb-3002cz and ps-3002cz. pb-3002cz (97,649 bp) displayed extensive sequence similarity with the blandm-1-carrying plasmid pkpx-1. ps-3002cz (73,581 bp) was found to consist of an incr-related sequence (13,535 bp) and a mosaic region (60,046 bp). a 40,233-bp sequence of ps-3002cz was identical to the mosaic region of pb-3002cz, indicating the en bloc acquisition of the ndm-1-encoding ... | 2015 | 25421477 |
| tigecycline for treatment of carbapenem-resistant klebsiella pneumoniae infections after liver transplantation in the intensive care unit: a 3-year study. | this 3-year prospective, observational, single-center study was undertaken to describe prescription, microbiology findings, tolerance, and efficacy of tigecycline for carbapenem-resistant klebsiella pneumoniae (crkp) infections after liver transplantation in the intensive care unit (icu). | 2014 | 25420864 |
| genetic profiles of fluoroquinolone-nonsusceptible klebsiella pneumoniae among cephalosporin-resistant k. pneumoniae. | the rate of fluoroquinolone (fq) resistance among the cephalosporin-resistant klebsiella pneumoniae is considerably high, however, their genetic profiles have not been well investigated. we selected 61 ciprofloxacin-nonsusceptible isolates from 102 k. pneumoniae isolates judged to be "resistant" to some cephalosporins during 2009 and 2012 throughout japan. pulsed-field gel electrophoresis excluded clonal isolates, and 29 isolates were subjected to multilocus sequence typing (mlst), detection of ... | 2015 | 25419619 |
| concomitant emphysematous prostatic and periurethral abscesses due to klebsiella pneumoniae: a case report and review of the literature. | although gas-forming infections of the urinary tract account for a very small percentage of all urinary tract infections, they can lead to mortality if an early diagnosis is not made and aggressive management initiated. emphysematous urinary tract infections occur mainly in patients with poorly controlled diabetes mellitus or an obstructed urinary tract. here we present a case of concomitant emphysematous prostatic and periurethral abscesses caused by klebsiella pneumoniae in a 70-year-old male ... | 2014 | 25417511 |
| the potential of selected south african plants with anti-klebsiella activity for the treatment and prevention of ankylosing spondylitis. | a wide variety of herbal remedies are used in traditional african medicine to treat inflammatory disorders, including some autoimmune diseases. thirty-four extracts from 13 south african plant species traditionally used for the treatment of inflammation were investigated for their ability to control a microbial trigger for ankylosing spondylitis (klebsiella pneumoniae). twenty-six of the extracts (76.5%) inhibited the growth of k. pneumoniae. methanol and water extracts of ballota africana, carp ... | 2015 | 25412961 |
| community-acquired invasive liver abscess syndrome caused by a k1 serotype klebsiella pneumoniae isolate in brazil: a case report of hypervirulent st23. | | 2014 | 25411006 |
| co-existence of blandm-1 and blakpc-2 in clinical isolates of klebsiella pneumoniae from pakistan. | | 2016 | 25407219 |
| pharmacodynamics of imipenem in combination with β-lactamase inhibitor mk7655 in a murine thigh model. | mk7655 is a newly developed beta-lactamase inhibitor of class a and class c carbapenemases. pharmacokinetics (pk) of imipenem-cilastatin (imp/c) and mk7655 were determined for intraperitoneal doses of 4 mg/kg to 128 mg/kg of body weight. mic and pharmacodynamics (pd) studies of mk7655 were performed against several beta-lactamase producing pseudomonas aeruginosa and klebsiella pneumoniae strains to determine its effect in vitro and in vivo. neutropenic mice were infected in each thigh 2 h before ... | 2015 | 25403667 |
| polymyxin b infusion leading to cardiac arrest: a case report and literature review. | we describe a 52-year-old female patient who developed cardiopulmonary arrest due to neuromuscular paralysis after initiation of polymyxin b for septicemia caused by extended spectrum β-lactamase-producing klebsiella pneumoniae. due to emerging antibiotic resistance patterns, use of polymyxins is increasing and an understanding of their toxicity is critical. | 2015 | 25398485 |
| cerebral air embolism. a case of disseminated klebsiella pneumoniae infection. | | 2014 | 25398120 |
| klebsiella pneumoniae bloodstream infection: epidemiology and impact of inappropriate empirical therapy. | multidrug resistance associated with extended-spectrum beta-lactamase (esbl) and klebsiella pneumoniae carbapenemase (kpc) among k. pneumoniae is endemic in southern europe. we retrospectively analyzed the impact of resistance on the appropriateness of empirical therapy and treatment outcomes of k. pneumoniae bloodstream infections (bsis) during a 2-year period at a 1420-bed tertiary-care teaching hospital in northern italy. we identified 217 unique patient bsis, including 92 (42%) kpc-positive, ... | 2014 | 25398065 |
| complete nucleotide sequence of klebsiella phage p13 and prediction of an eps depolymerase gene. | the complete genome of klebsiella phage p13 was sequenced and analyzed. bacteriophage p13 has a double-stranded linear dna with a length of 45,976 bp and a g+c content of 51.7 %, which is slightly lower than that of klebsiella pneumoniae kctc 2242. the codon biases of phage p13 are very similar to those of sp6-like phages and k. pneumoniae kctc 2242. bioinformatics analysis shows that the phage p13 genome has 282 open reading frames (orfs) that are greater than 100 bp in length, and 50 of these ... | 2015 | 25392088 |
| liver abscess associated with severe myopathy caused by klebsiella pneumoniae serotype k1 in romania. | liver abscess was diagnosed in a man presenting with fever, chills and severe myopathy. the k. pneumoniae isolated from blood cultures belonged to the k1 serotype. the patient responded favourably to percutaneous drainage of the abscess and antibiotics. this is the first documented report of klebsiella pneumoniae liver abscess syndrome (klas) described in romania and may indicate the emergence of this syndrome in eastern europe. | 2014 | 25390064 |
| first report in china of enterobacteriaceae clinical isolates coharboring blandm-1 and blaimp-4 drug resistance genes. | to describe the identification of two carbapenem-resistant, ndm-1 and imp-4, carbapenemases coproducing enterobacteriaceae isolates recovered from hospitalized patients in china. both klebsiella pneumoniae clinical isolates (kpn922 and kpn9599) were resistant to meropenem and imipenem and were subjected to additional antibiotic susceptibility testing. polymerase chain reaction (pcr) and sequence analyses were used to characterize bacterial carbapenemase resistance genes, extended-spectrum β-lact ... | 2015 | 25389598 |
| carbapenem-resistant klebsiella pneumoniae urinary tract infection following solid organ transplantation. | carbapenem-resistant klebsiella pneumoniae (crkp) is an emerging pathogen with a devastating impact on organ transplant recipients (otrs). data describing urinary tract infections (utis) due to crkp, compared to extended-spectrum β-lactamase (esbl)-producing and susceptible k. pneumoniae, are lacking. we conducted a retrospective cohort study comparing otrs with a first episode of uti due to crkp, esbl-producing k. pneumoniae, or susceptible k. pneumoniae. we identified 108 individuals; 22 (20%) ... | 2015 | 25385105 |
| comparison of decontamination methods for human skin grafts. | skin grafts intended for autologous transplant may be dropped on the operating room floor during handling. the authors examined optimal procedures for decontaminating tissue intended for burn surgery. porcine skin (5 × 5 cm sections) harvested from expired animals using standard procedures was inoculated with either 10(6) cfu/ml staphylococcus aureus or klebsiella pneumoniae. decontaminating strategies were compared: 10% povidone iodine, 0.04% chlorhexidine, or 50 u/ml bacitracin for injection, ... | 2015 | 25383978 |
| clavanin bacterial sepsis control using a novel methacrylate nanocarrier. | controlling human pathogenic bacteria is a worldwide problem due to increasing bacterial resistance. this has prompted a number of studies investigating peptides isolated from marine animals as a possible alternative for control of human pathogen infections. clavanins are antimicrobial peptides isolated from the marine tunicate styela clava, showing 23 amino acid residues in length, cationic properties, and also high bactericidal activity. in spite of clear benefits from the use of peptides, cur ... | 2014 | 25382976 |
| clinical outcomes of enterobacteriaceae infections stratified by carbapenem mics. | the clinical and laboratory standards institute (clsi) lowered the mic breakpoints for meropenem and imipenem from 4 mg/liter to 1 mg/liter for enterobacteriaceae in 2010. the breakpoint change improves the probability of pharmacodynamic target attainment and eliminates the need for microbiology labs to perform confirmatory testing for klebsiella pneumoniae carbapenemase (kpc) production or other beta-lactamases that hydrolyze carbapenems. however, there are limited data evaluating clinical outc ... | 2015 | 25378572 |
| high-dose tigecycline and colistin for successful treatment of disseminated carbapenem-resistant klebsiella pneumoniae infection in a liver transplant recipient. | solid organ transplantation (sot) is a risk factor for the acquisition of carbapenem-resistant klebsiella pneumoniae. this infection is associated with a high mortality rate given the limited armamentarium of antibiotics for multidrug-resistant organisms along with continued immunosuppression to prevent graft rejection. we report a case of carbapenem-resistant k. pneumoniae pneumonia, bacteraemia and intra-abdominal infection in a newly transplanted liver recipient. the patient was successfully ... | 2014 | 25378111 |
| control of carbapenemase-producing klebsiella pneumoniae: a region-wide intervention. | starting in 2010, there was a sharp increase in infections caused by klebsiella pneumoniae resistant to carbapenems in the emilia-romagna region in italy. a region-wide intervention to control the spread of carbapenemase-producing k. pneumoniae (cpkp) in emilia-romagna was carried out, based on a regional guideline issued in july 2011. the infection control measures recommended to the health trusts (hts) were: phenotypic confirmation of carbapenemase production, active surveillance of asymptomat ... | 2014 | 25375901 |
| efficiency of vanilla, patchouli and ylang ylang essential oils stabilized by iron oxide@c14 nanostructures against bacterial adherence and biofilms formed by staphylococcus aureus and klebsiella pneumoniae clinical strains. | biofilms formed by bacterial cells are associated with drastically enhanced resistance against most antimicrobial agents, contributing to the persistence and chronicization of the microbial infections and to therapy failure. the purpose of this study was to combine the unique properties of magnetic nanoparticles with the antimicrobial activity of three essential oils to obtain novel nanobiosystems that could be used as coatings for catheter pieces with an improved resistance to staphylococcus au ... | 2014 | 25375335 |
| prevalence of quinolone resistance among extended-spectrum β -lactamase producing uropathogenic klebsiella pneumoniae. | extended-spectrum β-lactamase (esbl) production is the major resistance mechanism to β-lactam antibiotics in enterobacteriaceae. in addition, emergence of plasmid-mediated quinolone resistance (pmqr) in esbl-producing isolates has become a global threat for treatment of these infections. | 2014 | 25371807 |
| quinolone susceptibility and detection of qnr and aac(6')-ib-cr genes in community isolates of klebsiella pneumoniae. | plasmid-mediated quinolone resistance genes (pmqr) have been shown to play not only an important role in quinolone resistance, but also resistance to other antibiotics, particularly β-lactams and aminoglycosides. these genes are mainly associated with clinical isolates of enterobacteriaceae. however, detection of pmqr genes in the community isolates can increase the dissemination rate of resistance determinants among bacteria. | 2014 | 25368793 |
| genomic epidemiology of klebsiella pneumoniae in italy and novel insights into the origin and global evolution of its resistance to carbapenem antibiotics. | klebsiella pneumoniae is at the forefront of antimicrobial resistance for gram-negative pathogenic bacteria, as strains resistant to third-generation cephalosporins and carbapenems are widely reported. the worldwide diffusion of these strains is of great concern due to the high morbidity and mortality often associated with k. pneumoniae infections in nosocomial environments. we sequenced the genomes of 89 k. pneumoniae strains isolated in six italian hospitals. strains were selected based on ant ... | 2015 | 25367909 |
| early carbapenem-resistant klebsiella pneumoniae bacteraemia: should we expand the screening? | | 2014 | 25366247 |
| in vitro screening and evaluation of some indian medicinal plants for their potential to inhibit jack bean and bacterial ureases causing urinary infections. | bacterial ureases play an important role in pathogenesis of urinary infections. selection of plants was done on the basis of their uses by the local people for the treatment of various bacterial and urinary infections. | 2015 | 25366133 |
| has the use of fluoroquinolones facilitated the widespread dissemination of methicillin-resistant staphylococcus aureus and extended-spectrum β-lactamase-producing klebsiella pneumoniae in the healthcare setting? | our group recently demonstrated that diverse fitness cost associated with resistance to fluoroquinolones allowed the extensive dissemination of the major international clones of both methicillin-resistant staphylococcus aureus (mrsa) and multiresistant klebsiella pneumoniae in the healthcare setting. the mechanism described by us was subsequently confirmed by british authors investigating the dynamics of mrsa clones in england. our results imply that the use of fluoroquinolones should impact the ... | 2014 | 25361527 |
| phenotypic and enzymatic comparative analysis of the kpc variants, kpc-2 and its recently discovered variant kpc-15. | sixteen different variants (kpc-2 to kpc-17) in the kpc family have been reported, and most current studies are focusing on kpc-2 and kpc-3. the kpc-15 variant, which isolated from klebsiella pneumoniae in a chinese hospital, was a recently discovered kpc enzyme. to compare the characteristics of kpc-15 and kpc-2, the variants were determined by susceptibility testing, pcr amplification and sequencing, and study of kinetic parameters. the strain harboring the kpc-15 showed resistance to 18 conve ... | 2014 | 25360633 |
| colistin resistance superimposed to endemic carbapenem-resistant klebsiella pneumoniae: a rapidly evolving problem in italy, november 2013 to april 2014. | consecutive non-replicate clinical isolates (n=191) of carbapenem non-susceptible enterobacteriaceae were collected from 21 hospital laboratories across italy from november 2013 to april 2014 as part of the european survey on carbapenemase-producing enterobacteriaceae (euscape) project. klebsiella pneumonia carbapenemase-producing k. pneumoniae (kpc-kp) represented 178 (93%) isolates with 76 (43%) respectively resistant to colistin, a key drug for treating carbapenamase-producing enterobacteriac ... | 2014 | 25358041 |
| emergence of klebsiella pneumoniae clinical isolates producing kpc-2 carbapenemase in cuba. | the emergence of klebsiella pneumoniae producing carbapenemase (kpc) has now become a global concern. as a part of a nationwide multicentre surveillance study in cuba, three k. pneumoniae clinical isolates resistant to carbapenems were detected for a 1-month period (september to october 2011). pcr and sequence analysis revealed that the three strains harboured bla kpc-2. they showed resistance or intermediate susceptibility to expanded-spectrum cephalosporins, other β-lactams, a β-lactam/β-lacta ... | 2014 | 25356357 |
| emergence of kpc-producing klebsiella pneumoniae in uruguay: infection control and molecular characterization. | we describe the first outbreak of klebsiella pneumoniae carbapenemase-producing k. pneumoniae (kpc-kp), the infection control measures adopted and the shift in resistance patterns of isolates during antibiotic treatment. the st258 kpc-kp strain exhibited a multiresistant antibiotic phenotype including co-resistance to gentamycin, colistin and tigecycline intermediate susceptibility. isolates before and after treatment had different behaviour concerning their antibiotic susceptibility and the pop ... | 2014 | 25356345 |
| kpc-producing klebsiella pneumoniae, finally targeting turkey. | we report here the first identification of the worldwide spread of klebsiella pneumoniae carbapenemase-2-producing and carbapenem-resistant k. pneumoniae clone st258 in turkey, a country where the distantly-related carbapenemase oxa-48 is known to be endemic. worryingly, this isolate was also resistant to colistin, now considered to be the last-resort antibiotic for carbapenem-resistant isolates. | 2014 | 25356342 |
| emergence of clinical klebsiella pneumoniae producing oxa-232 carbapenemase in singapore. | we report the emergence of oxa-232, a newly described oxa-48-like carbapenemase variant, in southeast asia. molecular characterization of eight klebsiella pneumoniae obtained from local and foreign patients reveals clonality of the isolates. bla oxa-232 was located on a non-conjugative plasmid of 6141 base pairs (genbank accession number jx423831.1). | 2013 | 25356318 |
| emerging k1 serotype klebsiella pneumoniae primary liver abscess: three cases presenting to a single university hospital in norway. | community-acquired klebsiella pneumoniae primary liver abscess (kla) has been emerging worldwide over the past two decades and with high incidence in asia. the presence of specific virulence characteristics is a risk factor for a syndrome with metastatic complications. this report signals an increasing emergence in northern europe. | 2014 | 25356268 |
| imipenem heteroresistance: high prevalence among enterobacteriaceae klebsiella pneumoniae carbapenemase producers. | | 2015 | 25351714 |
| gentamicin therapy for sepsis due to carbapenem-resistant and colistin-resistant klebsiella pneumoniae. | antimicrobial therapy for sepsis caused by carbapenem- and colistin-resistant klebsiella pneumoniae is not well established. we hypothesized that the early use of gentamicin in cases due to susceptible organisms would decrease the crude mortality rate of this infection. | 2015 | 25344809 |
| [risk factors for klebsiella pneumoniae carbapenemase-producing klebsiella pneumoniae colonization in neonates]. | to identify risk factors for klebsiella pneumoniae carbapenemase-producing klebsiella pneumoniae (kpc-kp) colonization in neonates hospitalized in the neonatal intensive care unit (nicu). | 2014 | 25344173 |
| carbapenemase-producing klebsiella pneumoniae. | the continuing emergence of infections due to multidrug resistant bacteria is a serious public health problem. klebsiella pneumoniae, which commonly acquires resistance encoded on mobile genetic elements, including ones that encode carbapenemases, is a prime example. k. pneumoniae carrying such genetic material, including both blakpc and genes encoding metallo-β-lactamases, have spread globally. many carbapenemase-producing k. pneumoniae are resistant to multiple antibiotic classes beyond β-lact ... | 2014 | 25343037 |
| klebsiella pneumoniae-induced liver abscesses, germany. | | 2014 | 25340973 |
| molecular pathogenesis of klebsiella pneumoniae. | typical klebsiella pneumoniae is an opportunistic pathogen, which mostly affects those with weakened immune systems and tends to cause nosocomial infections. a subset of hypervirulent k. pneumoniae serotypes with elevated production of capsule polysaccharide can affect previously healthy persons and cause life-threatening community-acquired infections, such as pyogenic liver abscess, meningitis, necrotizing fasciitis, endophthalmitis and severe pneumonia. k. pneumoniae utilizes a variety of viru ... | 2014 | 25340836 |
| predictors of therapy failure in a series of 741 adult pyogenic liver abscesses. | adult pyogenic liver abscess (pla) is a major hepato-biliary infection. we aim to identify risk factors associated with therapy failure. | 2015 | 25339111 |
| community-acquired invasive liver abscess syndrome caused by a k1 serotype klebsiella pneumoniae isolate in brazil: a case report of hypervirulent st23. | | 2014 | 25338152 |
| comorbidities and disease severity as risk factors for carbapenem-resistant klebsiella pneumoniae colonization: report of an experience in an internal medicine unit. | carbapenem-resistant klebsiella pneumoniae (crkp) is an emerging multidrug-resistant nosocomial pathogen, spreading to hospitalized elderly patients. risk factors in this setting are unclear. our aims were to explore the contribution of multi-morbidity and disease severity in the onset of crkp colonization/infection, and to describe changes in epidemiology after the institution of quarantine-ward managed by staff-cohorting. | 2014 | 25335100 |
| nitrile versus latex for glove juice sampling. | the objective of this study was to explore the utility of nitrile gloves as a replacement for latex surgical gloves in recovering bacteria from the hands. two types of nitrile gloves were compared to latex gloves using the parallel streak method. streaks of klebsiella pneumoniae and staphylococcus aureus were made on tryptic soy agar plates, and the zones of inhibition were measured around pieces of glove material placed on the plates. latex gloves produced a mean zone of inhibition of 0.28 mm, ... | 2014 | 25333880 |
| detection of carbapenemase production in gram-negative bacteria. | the greatest threat to antimicrobial treatment of infections caused by gram-negative bacteria is the production of carbapenemases. metallo-beta-lactamases and plasmid-mediated serine carbepenemases like klebsiella pneumonia carbapenemase are threatening the utility of almost all currently available beta-lactams including carbapenems. detection of organisms producing carbapenemases can be difficult, because their presence does not always produce a resistant phenotype on conventional disc diffusio ... | 2014 | 25328329 |
| identification and monitoring of nitrification and denitrification genes in klebsiella pneumoniae egd-hp19-c for its ability to perform heterotrophic nitrification and aerobic denitrification. | microbes capable of performing heterotrophic nitrification and aerobic denitrification simultaneously have application in nitrogen level management in effluent treatment plants. klebsiella pneumoniae egd-hp19-c is a metabolically versatile bacterium capable of utilising nh3-n, no2-n and no3-n as sole sources of nitrogen. the annotation was done for the genes involved in n-assimilation and n-dissimilation pathways from the draft genome sequences of this bacterium (ncbi genbank accession no. autw0 ... | 2015 | 25326064 |
| failure of a 5 day course of selective digestive decontamination solution in rectal decolonization of esbl-producing klebsiella pneumoniae in neonates. | | 2015 | 25324421 |
| molecular detection of antibiotic resistance genes from positive blood cultures. | rapid detection of the bacterial causative agent causing sepsis must be coupled with rapid identification of the antibiotic resistant mechanism that the pathogen might possess. real-time pcr (qpcr)-based assays have been extensively utilized in the clinical microbiology field as diagnostic tools for the rapid detection of specific nucleic acid (na) targets. in this chapter, we will discuss the technical aspects of using an internally controlled qpcr assay for the rapid detection of klebsiella pn ... | 2015 | 25319783 |
| risk factors for carbapenem resistant klebsiella pneumoniae rectal colonization in pediatric units. | | 2014 | 25313618 |
| infections by carbapenem-resistant klebsiella pneumoniae in sct recipients: a nationwide retrospective survey from italy. | infections by carbapenem-resistant klebsiella pneumoniae (crkp) represent a challenging problem after sct. a retrospective survey (january 2010 to july 2013) involving 52 italian centers was performed to assess the epidemiology and the prognostic factors of crkp infections in auto- and allo-sct. cases of crkp infection were reported in 53.4% of centers. crkp infections were documented in 25 auto-scts and 87 allo-scts, with an incidence of 0.4% (from 0.1% in 2010 to 0.7% in 2013) and 2% (from 0.4 ... | 2015 | 25310302 |
| on a boat: a case in australia of endophthalmitis and pyogenic liver, prostatic, and lung abscesses in a previously well patient due to klebsiella pneumoniae. | this is a case report about a patient who arrived in our emergency department in western australia to the care of the urologists having just gotten off a ship with a bacterial infection that would result in a 44-day stay in hospital and have quite devastating lasting effects for the young male. his story was in fact reflective of an emerging global phenomenon. once thought to generally be a bacterium of threat only to the elderly and alcoholics, causing pneumonia and urinary tract infections, th ... | 2014 | 25309763 |
| a descriptive study of nosocomial infections in an adult intensive care unit in fiji: 2011-12. | nosocomial infections in an intensive care unit (icu) are common and associated with a high mortality but there are no published data from the oceania region. a retrospective study in fiji's largest icu (2011-12) reported that 114 of a total 663 adult icu admissions had bacteriological culture-confirmed nosocomial infection. the commonest sites of infection were respiratory and bloodstream. gram negative bacteria were the commonest pathogens isolated, especially klebsiella pneumoniae (extended-s ... | 2014 | 25309601 |
| evaluation of a new chromogenic medium, chromid oxa-48, for recovery of carbapenemase-producing enterobacteriaceae from patients at a university hospital in turkey. | the purpose of this study was to evaluate a new chromogenic medium, chromid oxa-48, for the isolation of carbapenemase-producing enterobacteriaceae (cpe) directly from rectal swabs. chromid carba and chromid oxa-48 are two chromogenic media that have been commercialized for the isolation of cpe directly from clinical samples. both media were evaluated alongside a broth enrichment method recommended by the cdc for isolation of cpe, with rectal swabs from 302 unique hospitalized patients at the ha ... | 2015 | 25308827 |
| misidentification of pan drug-resistant klebsiella pneumoniae clinical isolates as a metallo-β-lactamase producers by the edta/ddst test. | | 2015 | 25307681 |
| polymyxin b with dual carbapenem combination therapy against carbapenemase-producing klebsiella pneumoniae. | | 2015 | 25305144 |
| thrombocytopenia impairs host defense in gram-negative pneumonia-derived sepsis in mice. | thrombocytopenia is a common finding in sepsis and associated with a worse outcome. we used a mouse model of pneumonia-derived sepsis caused by the human pathogen klebsiella pneumoniae to study the role of platelets in host response to sepsis. platelet counts (pcs) were reduced to less than a median of 5 × 10(9)/l or to 5 to 13 × 10(9)/l by administration of a depleting antibody in mice infected with klebsiella via the airways. thrombocytopenia was associated with strongly impaired survival duri ... | 2014 | 25301709 |
| cellulolytic activity and structure of symbiotic bacteria in locust guts. | locusts are able to digest the cellulose of gramineae plants, resulting in their being considered as major crop pests. to illustrate the mechanism involved in cellulose digestion, the cellulolytic activity and zymography in the gut contents of 16 locust species were determined using carboxymethyl cellulose (cmc) as substrate. the diversity of gut symbiotic bacteria was studied using denaturing gradient gel electrophoresis (dgge). the results showed that high cmc activity was present in acrididae ... | 2014 | 25299108 |
| klebsiella pneumoniae carrying bla ndm-1 gene in orthopedic practice. | emergence and spread of carbapenemases in enterobacteriaceae is a cause of concern worldwide, the latest threat being new delhi metallo-β-lactamase (ndm-1). this report is of an orthopedic case with fracture femur managed with internal fixation and bone grafting, who subsequently developed secondary infection with klebsiella pneumoniae harboring bla ndm-1 gene. minimum inhibitory concentration (mic) of imipenem was ≥8 μg/ml by e-test, suggestive of carbapenemase production. phenotypic and furthe ... | 2014 | 25298566 |
| a possible alternative to the error prone modified hodge test to correctly identify the carbapenemase producing gram-negative bacteria. | the modified hodge test (mht) is widely used as a screening test for the detection of carbapenemases in gram-negative bacteria. this test has several pitfalls in terms of validity and interpretation. also the test has a very low sensitivity in detecting the new delhi metallo-β-lactamase (ndm). considering the degree of dissemination of the ndm and the growing pandemic of carbapenem resistance, a more accurate alternative test is needed at the earliest. | 2014 | 25297027 |
| the prevalence of infections and colonisation with klebsiella pneumoniae strains isolated in icu patients. | klebsiella spp. are among the bacteria most commonly isolated from patients with infections in icus. the source of these infections may be the microflora of the patient or the hospital environment. increasingly, klebsiella strains are also being isolated from epidemic outbreaks. this situation is largely the result of widespread, irrational antibiotic use, the virulence of the bacterial strains and their ability to survive in the hospital environment. the purpose of this dissertation was to esti ... | 2014 | 25293479 |
| draft genome assembly of klebsiella pneumoniae type strain atcc 13883. | klebsiella pneumoniae is a common cause of antibiotic-resistant bacterial infections in immunocompromised individuals. here, we present the 5.54-mb scaffolded assembly of the type strain k. pneumoniae type strain atcc 13883, as deposited in genbank under accession no. joow00000000. | 2014 | 25291763 |
| complete genome sequence of klebsiella pneumoniae strain atcc 43816 kppr1, a rifampin-resistant mutant commonly used in animal, genetic, and molecular biology studies. | klebsiella pneumoniae is an urgent public health threat due to the spread of carbapenem-resistant strains causing serious, and frequently fatal, infections. to facilitate genetic, molecular, and immunological studies of this pathogen, we report the complete chromosomal sequence of a genetically tractable, prototypical strain used in animal models. | 2014 | 25291761 |
| sputum bacteriology and antibiotic sensitivity patterns of community-acquired pneumonia in hospitalized adult patients in nigeria: a 5-year multicentre retrospective study. | a clear knowledge of the pathogens responsible for community-acquired pneumonia (cap) in a given region and their antibiotic sensitivity patterns is necessary for optimal treatment. we determined the common bacterial pathogens causing cap in nigeria and further reviewed their antibiotic senstivity patterns with a view to providing recommendations to improve antibiotic management of cap. | 2014 | 25288384 |
| kpc-producing klebsiella pneumoniae strains that harbor aac(6')-ib exhibit intermediate resistance to amikacin. | the aminoglycoside-modifying enzyme aac(6')-ib is common among carbapenem-resistant klebsiella pneumoniae (cr-kp) strains. we investigated amikacin (amk) activity against 20 aac(6')-ib-producing cr-kp strains. mics clustered at 16 to 32 μg/ml. by the time-kill study, amk (1× and 4× the mic) was bactericidal against 30% and 85% of the strains, respectively. at achievable human serum concentrations, however, the majority of strains showed regrowth, suggesting that aac(6')-ib confers intermediate a ... | 2014 | 25288089 |
| crystal structures of klebsiella pneumoniae dihydrofolate reductase bound to propargyl-linked antifolates reveal features for potency and selectivity. | resistance to the antibacterial antifolate trimethoprim (tmp) is increasing in members of the family enterobacteriaceae, driving the design of next-generation antifolates effective against these gram-negative pathogens. the propargyl-linked antifolates are potent inhibitors of dihydrofolate reductases (dhfr) from several tmp-sensitive and -resistant species, including klebsiella pneumoniae. recently, we have determined that these antifolates inhibit the growth of strains of k. pneumoniae, some w ... | 2014 | 25288083 |
| high prevalence of metallo-β-lactamase among carbapenem-resistant klebsiella pneumoniae in a teaching hospital in china. | the aim of this study was to characterize the carbapenemases in carbapenem-resistant klebsiella pneumoniae (cr-kp) from a chinese teaching hospital. a total of 40 cr-kps were screened for the presence of carbapenemases. minimum inhibitory concentrations were determined by agar dilution. the modified hodge test was used for the detection of carbapenemase production. carbapenemase, extended-spectrum β-lactamase, and ampc genes were detected using polymerase chain reaction (pcr) and sequencing. a c ... | 2014 | 25285938 |
| osteomyelitis due to multiple carbapenemase-producing gram-negative bacteria: the first case report of a ges-13-producing pseudomonas aeruginosa isolate in canada. | a case of osteomyelitis in an infant following a burn injury sustained in pakistan caused by a ges-13-producing pseudomonas aeruginosa (the first reported in canada) and an oxa-48 producing klebsiella pneumoniae is described. the present case serves to highlight the importance of international travel as a risk factor for infection with carbapenemase-producing bacteria and the challenges in the laboratory detection of these organisms. | 2014 | 25285131 |
| susceptibility to klebsiella pneumonaie infection in collaborative cross mice is a complex trait controlled by at least three loci acting at different time points. | klebsiella pneumoniae (kp) is a bacterium causing severe pneumonia in immunocompromised hosts and is often associated with sepsis. with the rise of antibiotic resistant bacteria, there is a need for new effective and affordable control methods; understanding the genetic architecture of susceptibility to kp will help in their development. we performed the first quantitative trait locus (qtl) mapping study of host susceptibility to kp infection in immunocompetent collaborative cross mice (cc). we ... | 2014 | 25283706 |
| what may be lurking in the hospital undergrowth? inapparent cross-transmission of extended-spectrum beta-lactamase-producing klebsiella pneumoniae. | extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae pose an increasing challenge in hospitals. | 2014 | 25280879 |
| genome sequencing and genomic characterization of a tigecycline-resistant klebsiella pneumoniae strain isolated from the bile samples of a cholangiocarcinoma patient. | the relationship between klebsiella pneumoniae and nosocomial and community-acquired infections is well known, and k. pneumoniae resistance to most antibiotics is increasing worldwide. in contrast, tigecycline remains active against many bacterial strains, and serves as a last resort for treating multi-drug resistant bacterial infections. that tigecycline nonsusceptibility among k. pneumoniae isolates has been reported worldwide is worrying. however, the mechanisms of tigecycline resistance in k ... | 2014 | 25279004 |
| endogenous endophthalmitis complicated by pyogenic liver abscess: a review of 17 years' experience at a single center. | endogenous endophthalmitis is a rare complication of pyogenic liver abscess. it is a devastating intraocular infection which constitutes a vision-threatening emergency. recently, a significant increase in the incidence of endogenous endophthalmitis associated with pyogenic liver abscess has been reported in east asia. in this study, the authors investigated the incidence, risk factors, clinical features, and treatment outcomes of endogenous endophthalmitis arising as a complication of pyogenic l ... | 2014 | 25277733 |
| multiplex pcr for detection of seven virulence factors and k1/k2 capsular serotypes of klebsiella pneumoniae. | a single multiplex pcr assay targeting seven virulence factors and the wzi gene specific for the k1 and k2 capsular serotypes of klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. the assay is useful for the surveillance of emerging highly virulent strains. | 2014 | 25275000 |
| production of optically pure d-lactate from glycerol by engineered klebsiella pneumoniae strain. | in this study, glycerol was used to produce optically pure d-lactate by engineered klebsiella pneumoniae strain. in the recombinant strain, d-lactate dehydrogenase ldha was overexpressed, and two genes, dhat and yqhd for biosynthesis of main byproduct 1,3-propanediol, were knocked out. to further improve d-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in d-lactate production. in microaerobic fed-batch fermentation ... | 2014 | 25270041 |
| comparison of multilocus sequence typing, rapd, and maldi-tof mass spectrometry for typing of β-lactam-resistant klebsiella pneumoniae strains. | extended spectrum of β-lactam (esbl) resistance of klebsiella pneumoniae has become an increasing problem in hospital infections. typing of isolates is important to establish the intrahospital surveillance of resistant clones. in this study, the discriminatory potential of randomly amplified polymorphic dna and matrix-assisted laser desorption ionization-time of flight (maldi-tof) analyses were compared with multilocus sequence typing (mlst) by using 17 β-lactam-resistant k. pneumoniae isolates ... | 2014 | 25266674 |
| bloodstream infections in pediatric patients at cancer institute, chennai. | there is paucity of data on the epidemiology of bloodstream infections in pediatric cancer patients from india. rationale use of antibiotics in febrile neutropenia is important for reducing morbidity and preventing the emergence of drug resistant bacteria. | 2016 | 26842144 |
| evaluation of inhibitory action of novel non β-lactam inhibitor against klebsiella pneumoniae carbapenemase (kpc-2). | the use of three classical β-lactamase inhibitors (clavulanic acid, tazobactam and sulbactam) in combination with β-lactam antibiotics is presently the mainstay of antibiotic therapy against gram-negative bacterial infections. however these inhibitors are unable to inhibit carbapenemase kpc-2 effectively. they being β-lactam derivatives behave as substrates for this enzyme instead of inactivating it. we have initiated our study to check the in vitro inhibition activity of the two novel screened ... | 2014 | 25265157 |
| application of zwitterionic detergent to the solubilization of klebsiella pneumoniae outer membrane proteins for two-dimensional gel electrophoresis. | klebsiella pneumoniae is a frequent cause of nosocomial respiratory, urinary and gastrointestinal tract infections and septicemia with the multidrug-resistant k. pneumoniae being a major public health concern. outer membrane proteins (omps) are important virulence factors responsible for the appropriate adaptation to the host environment. they constitute of the antigens being the first in contact with infected organism. however, k. pneumoniae strains are heavily capsulated and it is important to ... | 2014 | 25261774 |
| klebsiella pneumoniae yfirnb operon affects biofilm formation, polysaccharide production and drug susceptibility. | klebsiella pneumoniae is an opportunistic pathogen important in hospital-acquired infections, which are complicated by the rise of drug-resistant strains and the capacity of cells to adhere to surfaces and form biofilms. in this work, we carried out an analysis of the genes in the k. pneumoniae yfirnb operon, previously implicated in biofilm formation. the results indicated that in addition to the previously reported effect on type 3 fimbriae expression, this operon also affected biofilm formati ... | 2014 | 25261190 |