| ventilator-associated pneumonia caused by colistin-resistant kpc-producing klebsiella pneumoniae: a case report and literature review. | klebsiella pneumoniae producing kpc-type carbapenemase causes severe nosocomial infection at a high mortality rate. nosocomial pneumonia in particular is associated with high mortality, likely due to the unfavorable pulmonary pharmacokinetics of the antibiotics used against this agent. therefore, early and accurate microbiological identification and susceptibility evaluation are crucial in order to optimize antibiotic therapy. we report a case of ventilator-associated pneumonia caused by colisti ... | 2015 | 25951099 |
| klebsiella pneumoniae and the pyogenic liver abscess: implications and association of the presence of rpma genes and expression of hypermucoviscosity. | | 2015 | 25951089 |
| klebsiella pneumoniae in septicemic neonates with special reference to extended spectrum β-lactamase, ampc, metallo β-lactamase production and multiple drug resistance in tertiary care hospital. | β-lactamases viz., extended spectrum β-lactamase (esbl), ampc, and metallo β-lactamase (mbl) production in klebsiella pneumoniae has led to a serious concern about septicemic neonates in neonatal intensive care units due to high resistance against commonly used antimicrobials. | 2017 | 25949057 |
| the role of ompk35, ompk36 porins, and production of β-lactamases on imipenem susceptibility in klebsiella pneumoniae clinical isolates, cairo, egypt. | ompk35 and ompk36 are the major outer membrane porins of klebsiella pneumoniae. we aimed to study the effect of combined porin loss and production of extended-spectrum β-lactamases (esbls) on imipenem susceptibility among k. pneumoniae clinical isolates. | 2015 | 25945596 |
| pneumonia and bacteremia in a golden-headed lion tamarin (leontopithecus chrysomelas) caused by klebsiella pneumoniae subsp. pneumoniae during a translocation program of free-ranging animals in brazil. | klebsiella pneumoniae is an important emerging pathogen in humans, particularly the invasive hypermucoviscosity (hmv) phenotype. in addition, the organism is an important public health concern because of nosocomial infections and antimicrobial resistance. nonhuman primates in captivity are susceptible to klebsiella, particularly when a stress factor is involved. infections vary depending on the species but can cause significant morbidity and mortality in these animals. the objective of this stud ... | 2015 | 25943130 |
| neonatal nosocomial bloodstream infections at a referral hospital in a middle-income country: burden, pathogens, antimicrobial resistance and mortality. | data on nosocomial bloodstream infection (bsi) rates, pathogens, mortality and antimicrobial resistance in african neonates are limited. | 2015 | 25940506 |
| differentiation of incl and incm plasmids associated with the spread of clinically relevant antimicrobial resistance. | blaoxa-48, blandm-1 and blactx-m-3 are clinically relevant resistance genes, frequently associated with the broad-host range plasmids of the incl/m group. the l and m plasmids belong to two compatible groups, which were incorrectly classified together by molecular methods. in order to understand their evolution, we fully sequenced four incl/m plasmids, including the reference plasmids r471 and r69, the recently described blaoxa-48-carrying plasmid pkpn-el.nr7 from a klebsiella pneumoniae isolate ... | 2015 | 25933288 |
| treeseq, a fast and intuitive tool for analysis of whole genome and metagenomic sequence data. | next-generation sequencing is not yet commonly used in clinical laboratories because of a lack of simple and intuitive tools. we developed a software tool (treeseq) with a quaternary tree search structure for the analysis of sequence data. this permits rapid searches for sequences of interest in large datasets. we used treeseq to screen a gut microbiota metagenomic dataset and a whole genome sequencing (wgs) dataset of a strain of klebsiella pneumoniae for antibiotic resistance genes and compare ... | 2015 | 25933115 |
| the first klebsiella pneumoniae isolate co-producing oxa-48 and ndm-1 in turkey. | | 2015 | 25932453 |
| disrupting the mixed-species biofilm of klebsiella pneumoniae b5055 and pseudomonas aeruginosa pao using bacteriophages alone or in combination with xylitol. | we investigated the potential of bacteriophages alone as well as in combination with xylitol for tackling mixed-species biofilm of pseudomonas aeruginosa and klebsiella pneumoniae. when mixed-species biofilm was established on polycarbonate discs, p. aeruginosa formed the base layer which was physically shielded on the top by k. pneumoniae. thereafter, mixed-species biofilm was treated with bacteriophages. k. pneumoniae-specific depolymerase-producing phage kpo1k2 caused significant reduction in ... | 2015 | 25922418 |
| role of cd64 expression on neutrophils in the diagnosis of sepsis and the prediction of mortality in adult critically ill patients. | intensive care unit patients who developed systemic inflammatory response syndrome (sirs) with proven microbial etiology were assigned to the infectious causes (n = 29), while patients with negative cultures and more probable other etiology were assigned to the noninfectious causes (n = 37). flow cytometry was used to detect the presence of cd64 on neutrophils. the multivariate analysis revealed that kpc-producing klebsiella pneumoniae rectal colonization and >1.39 mean fluorescence intensity (m ... | 2015 | 25921729 |
| covalent immobilization of pullulanase on alginate and study of its hydrolysis of pullulan. | the immobilization of pullulanase from klebsiella pneumoniae by grafting was investigated. pullulanase was linked after activation of alginate via a covalent bond between the amine groups of the enzyme and the carboxylic acid groups of alginate. the immobilization yield was 60%. the activity of free pullulanase and immobilized pullulanase was followed by the quantification of reducing ends by colorimetric assay and the determination of the molar masses of the hydrolyzed pullulan by sec/mals/dri. ... | 2015 | 25919860 |
| exploring the role of residue 228 in substrate and inhibitor recognition by vim metallo-β-lactamases. | β-lactamase inhibitors (blis) restore the efficacy of otherwise obsolete β-lactams. however, commercially available blis are not effective against metallo-β-lactamases (mbls), which continue to be disseminated globally. one group of the most clinically important mbls is the vim family. the discovery of vim-24, a natural variant of vim-2, possessing an r228l substitution and a novel phenotype, compelled us to explore the role of this position and its effects on substrate specificity. we employed ... | 2015 | 25915520 |
| molecular epidemiology of carbapenemase-producing klebsiella pneumoniae in a hospital in madrid: successful establishment of an oxa-48 st11 clone. | here we report a retrospective clinical and molecular study conducted in a tertiary care facility in southern madrid, spain, from january 2009 to february 2014 to investigate the epidemiology of carbapenemase-producing klebsiella pneumoniae (cpkp). carbapenemase genes were identified in 97 non-duplicate k. pneumoniae isolates, including 59 harbouring blaoxa-48, 37 harbouring blavim-1 and 1 harbouring blakpc-2. pulsed-field gel electrophoresis (pfge) analysis verified the presence of 20 different ... | 2015 | 25914088 |
| faecal microbiota transplantation for stool decolonization of oxa-48 carbapenemase-producing klebsiella pneumoniae. | | 2015 | 25913649 |
| kpc-producing klebsiella pneumoniae rectal colonization is a risk factor for mortality in patients with diabetic foot infections. | to evaluate the relationship between carbapenemase-producing klebsiella pneumoniae (kpc-kp) gut colonization and mortality in diabetic patients with a foot infection (dfi) we performed a single-centre, retrospective, matched case-control study. in the study period, we identified 21 patients with dfi who had kpc-kp gut colonization and 21 controls. the 90-day mortality rate was significantly higher in patients with colonized guts (47%) than the controls (4%) (p 0.013). a multivariate analysis dem ... | 2015 | 25911991 |
| whole genome sequencing of extended-spectrum β-lactamase producing klebsiella pneumoniae isolated from a patient in lebanon. | the emergence of extended-spectrum β-lactamase (esbl)-producing bacteria is now a critical concern. the esbl-producing klebsiella pneumoniae constitutes one of the most common multidrug-resistant (mdr) groups of gram-negative bacteria involved in nosocomial infections worldwide. in this study we report on the molecular characterization through whole genome sequencing of an esbl-producing k. pneumoniae strain, lau-kp1, isolated from a stool sample from a patient admitted for a gastrointestinal pr ... | 2015 | 25905047 |
| infections caused by kpc-producing klebsiella pneumoniae: differences in therapy and mortality in a multicentre study. | infections caused by klebsiella pneumoniae (kp) carbapenemase (kpc)-producing strains of kp have become a significant threat in recent years. to assess their outcomes and identify risk factors for 14 day mortality, we conducted a 4 year (2010-13) retrospective cohort study in five large italian teaching hospitals. | 2015 | 25900159 |
| characterization of pre-antibiotic era klebsiella pneumoniae isolates with respect to antibiotic/disinfectant susceptibility and virulence in galleria mellonella. | the egd murray collection consists of approximately 500 clinical bacterial isolates, mainly enterobacteriaceae, isolated from around the world between 1917 and 1949. a number of these "murray" isolates have subsequently been identified as klebsiella pneumoniae. antimicrobial susceptibility testing of these isolates showed that over 30% were resistant to penicillins due to the presence of diverse blashv β-lactamase genes. analysis of susceptibility to skin antiseptics and triclosan showed that wh ... | 2015 | 25896708 |
| activity of antimicrobial combinations against kpc-2-producing klebsiella pneumoniae in a rat model and time-kill assay. | this study evaluated the efficacy of tigecycline (tig), polymyxin b (pmb), and meropenem (mer) in 80 rats challenged with klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae infection. a time-kill assay was performed with the same strain. triple therapy and pmb+tig were synergistic, promoted 100% survival, and produced negative peritoneal cultures, while mer+tig showed lower survival and higher culture positivity than other regimens (p = 0.018) and was antagonistic. in vivo and in ... | 2015 | 25896686 |
| population pharmacokinetics and pharmacodynamics of piperacillin/tazobactam in patients with nosocomial infections. | the study was to establish a population pharmacokinetic (ppk) model of piperacillin (pip) and tazobactam (taz) that explain pharmacokinetic variability and to propose optimized dosage regimens in patients with nosocomial infections. | 2016 | 25894901 |
| identification of outer membrane and exoproteins of carbapenem-resistant multilocus sequence type 258 klebsiella pneumoniae. | carbapenem-resistant klebsiella pneumoniae strains have emerged as a cause of life-threatening infections in susceptible individuals (e.g., transplant recipients and critically ill patients). strains classified as multilocus sequence type (st) 258 are among the most prominent causes of carbapenem-resistant k. pneumoniae infections worldwide, but the basis for the success of this lineage remains incompletely determined. to gain a more comprehensive view of the molecules potentially involved in th ... | 2015 | 25893665 |
| effects of an anti-inflammatory vap-1/ssao inhibitor, pxs-4728a, on pulmonary neutrophil migration. | the persistent influx of neutrophils into the lung and subsequent tissue damage are characteristics of copd, cystic fibrosis and acute lung inflammation. vap-1/ssao is an endothelial bound adhesion molecule with amine oxidase activity that is reported to be involved in neutrophil egress from the microvasculature during inflammation. this study explored the role of vap-1/ssao in neutrophilic lung mediated diseases and examined the therapeutic potential of the selective inhibitor pxs-4728a. | 2015 | 25889951 |
| bufei jianpi granules improve skeletal muscle and mitochondrial dysfunction in rats with chronic obstructive pulmonary disease. | bufei jianpi granules has been confirmed effective in improving pulmonary function, alleviating acute exacerbations, improving six-minute walk distance and quality of life, and benefited in 12-month follow-up in chronic obstructive pulmonary disease (copd) patients with syndrome of lung-spleen qi deficiency. skeletal muscle dysfunction (smd), an important extrapulmonary complication, occurs in the very initiation of copd and is closely related to morbidity and mortality. to evaluate the efficacy ... | 2015 | 25888379 |
| clinical and microbiological characteristics of klebsiella pneumoniae liver abscess in east china. | klebsiella pneumoniae has been the dominant pathogen for liver abscesses in several asian countries. although the prevalence of k. pneumoniae liver abscess (kla) in mainland china is increasing recently, the clinical and microbiological characteristics of kla in china have not been elucidated. | 2015 | 25886859 |
| evaluation of the efficacy of a bacteriophage in the treatment of pneumonia induced by multidrug resistance klebsiella pneumoniae in mice. | multidrug-resistant klebsiella pneumoniae (mrkp) has steadily grown beyond antibiotic control. however, a bacteriophage is considered to be a potential antibiotic alternative for treating bacterial infections. in this study, a lytic bacteriophage, phage 1513, was isolated using a clinical mrkp isolate kp 1513 as the host and was characterized. it produced a clear plaque with a halo and was classified as siphoviridae. it had a short latent period of 30 min, a burst size of 264 and could inhibit k ... | 2015 | 25879036 |
| population structure of klebsiella pneumoniae causing bloodstream infections at a new york city tertiary care hospital: diversification of multidrug-resistant isolates. | despite the growing importance of carbapenem-resistant klebsiella pneumoniae (crkp), the clonal relationships between crkp and antibiotic-susceptible isolates remain unclear. we compared the genetic diversity and clinical features of crkp, third-generation and/or fourth-generation cephalosporin-resistant (ceph-r) k. pneumoniae, and susceptible k. pneumoniae isolates causing bloodstream infections at a tertiary care hospital in new york city between january 2012 and july 2013. drug susceptibiliti ... | 2015 | 25878348 |
| effect of carbon sources on the catalytic performance of ni/β-mo2c. | in this paper, ni/β-mo2c(s) and ni/β-mo2c(g) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (mfcs). the carburized catalyst samples were characterized by x-ray diffraction (xrd), scanning electron microscope (sem), and brunauer-emmett-teller (bet). the activity of the electrocatalyst towards the oxidation of several common microbial fermentation products (form ... | 2015 | 25877400 |
| [controlling infection and spread of carbapenems-resistant klebsiella pneumoniae among burn patients]. | the emergence and spread of carbapenems-resistant klebsiella pneumoniae (crkp) in burn ward is an important threat to burn management. crkp isolates are resistant to almost all available antibiotics and are susceptible only to polymyxins and tigecycline. the mechanism of the drug resistance of crkp is associated with the plasmid-encoded carbapenemase klebsiella pneumoniae carbapenemase (kpc), a carbapenem-hydrolyzing β-lactamase. antibiotics which can currently be used to treat crkp infection in ... | 2015 | 25876631 |
| accuracy of in vitro susceptibility tests for carbapenemase-producing gram-negative bacteria. | accurate susceptibility results on antibiotic-resistant bacteria are essential for proper treatment of infections. in this study, 100 metallo-β-lactamase (mbl)-producing strains and 95 isolates with klebsiella pneumoniae carbapenemase (kpc) were tested for carbapenem susceptibility using two automated platforms, the phoenix and vitek-2 systems, and a manual etest. phoenix showed higher categorical agreements (97% for imipenem and 94% for meropenem) compared with those from vitek-2 (92 and 74%) a ... | 2015 | 25873580 |
| proton pump inhibitor use significantly increases the risk of cryptogenic liver abscess: a population-based study. | proton pump inhibitors (ppis) increase gastric ph and impair defence mechanisms against ingested pathogens, which may result in the overgrowth of virulent klebsiella pneumoniae in the intestine and subsequent liver abscess. | 2015 | 25871643 |
| utility of whole-cell repetitive extragenic palindromic sequence-based pcr (rep-pcr) for the rapid detection of nosocomial outbreaks of multidrug resistant organisms: experience at a tertiary care center in north india. | there is a huge need to develop molecular typing methods which are simple to perform, rapid and cost effective to confirm clonality of nosocomial isolates in outbreak situations. | 2015 | 25865971 |
| an outbreak of infections caused by extensively drug-resistant klebsiella pneumoniae strains during a short period of time in a chinese teaching hospital: epidemiology study and molecular characteristics. | in this study, we comprehensively described the clinical risk factors, outcome, epidemiology, and molecular basis associated with an outbreak of extensively drug-resistant kpc-2-producing klebsiella pneumoniae involving 15 patients in a teaching hospital from may 1 to june 27, 2013. most of the patients were elderly and received long-term hospital treatment, and 40.0% (6/15) of them were dead. all strains carried bla(kpc-2), rmtb, bla(ctx-m-65), bla(shv-11), oqxa, oqxb, and aac(6')-ib-cr and eve ... | 2015 | 25865067 |
| management of carbapenem resistant klebsiella pneumoniae infections in stem cell transplant recipients: an italian multidisciplinary consensus statement. | | 2015 | 25862702 |
| detection of bla kpc-2 in proteus mirabilis in brazil. | infections caused by klebsiella pneumoniae carbapenemase (kpc)-producing isolates pose a major worldwide public health problem today. | 2015 | 25860472 |
| role of triggering receptor expressed on myeloid cells-1/3 in klebsiella-derived pneumosepsis. | triggering receptor expressed on myeloid cells (trem)-1 and -2 can affect toll-like receptor-mediated activation of immune cells. klebsiella pneumoniae is a common cause of pneumonia-derived sepsis. here we studied the role of trem-1/3 and trem-2 in the host response during klebsiella pneumonia. macrophages lacking either trem-1/3 or trem-2 were tested for their responsiveness toward k. pneumoniae and for their capacity to internalize this pathogen in vitro. trem-1/3- and trem-2-deficient mice w ... | 2015 | 25860078 |
| antimicrobial activity of pomegranate fruit constituents against drug-resistant mycobacterium tuberculosis and β-lactamase producing klebsiella pneumoniae. | the global surge in multi-drug resistant bacteria and the imminence of tuberculosis pandemic necessitate alternative therapeutic approaches to augment the existing medications. pomegranate, the fruit of punica granatum linn. (punicaceae), widely recognized for potency against a broad spectrum of bacterial pathogens, deserves further investigation in this respect. | 2015 | 25858784 |
| a suggested new bacteriophage genus, "kp34likevirus", within the autographivirinae subfamily of podoviridae. | klebsiella pneumoniae phages vb_kpnp_su503 (su503) and vb_kpnp_su552a (su552a) are virulent viruses belonging to the autographivirinae subfamily of podoviridae that infect and kill multi-resistant k. pneumoniae isolates. phages su503 and su552a show high pairwise nucleotide identity to klebsiella phages kp34 (nc_013649), f19 (nc_023567) and ntuh-k2044-k1-1 (nc_025418). bioinformatic analysis of these phage genomes show high conservation of gene arrangement and gene content, conserved catalytical ... | 2015 | 25853484 |
| prevalence assessment of maga gene and antimicrobial susceptibility of klebsiella pneumoniae isolated from clinical specimens in shahrekord, iran. | klebsiella pneumoniae (k. pneumoniae) is an opportunistic microorganism. this study aimed to investigate the presence of maga gene and antimicrobial susceptibility in k. pneumoniae. | 2014 | 25848520 |
| [periplasmic lysozime inhibitior plic and its role in antilysozime activity of enterobacteria]. | two families of specific inhibitors of type c lysozyme (ivy and plic) secreted from the periplasmic space are known in enterobacteria. microbial capacity for distant lysozyme inactivation (antilysozyme activity) is most pronounced in the strains and species carrying homologues of the plicgene. the plic homologue localized in a -200-kbp megaplasmid of klebsiella pneumoniae was shown to differ significantly in the amino acid composition of the coded polypeptide. similar to the salmonella enterica ... | 2015 | 25844440 |
| associations of the major international high-risk resistant clones and virulent clones with specific ompk36 allele groups in klebsiella pneumoniae in taiwan. | this study was conducted to investigate the association between ompk36 variants and international high-risk clones in klebsiella pneumoniae. fifty-nine sequence types (sts) divided into four ompk36 allele groups (groups a to d) were identified among 185 k. pneumoniae isolates. the major high-risk clones (29 st11, 13 st15, 7 st37 and 1 st147 isolates) were assigned to group a, while 6 sts (15 st23, 2 st65, 3 st86, 1 st163, 1 st373 and 2 st375 isolates) associated with pyogenic liver abscess were ... | 2015 | 25834737 |
| low prevalence of rmpa and high tendency of rmpa mutation correspond to low virulence of extended spectrum β-lactamase-producing klebsiella pneumoniae isolates. | invasive syndrome caused by klebsiella pneumoniae (kp), including liver abscess, is mainly caused by community-acquired strains with characteristics of positive hypermucoviscosity (hv) phenotype and regulator of mucoid phenotype a (rmpa) and transcriptional activator (rmpa2) genes. extended- spectrum β-lactamase-producing kp (esbl-kp) is commonly nosocomial and rarely hv-positive. we aimed to explore the reasons of the rarer prevalence of hv phenotype, rmpa and rmpa2 as well as the virulence phe ... | 2015 | 25830726 |
| first detection of kpc-3-producing klebsiella pneumoniae in albania. | | 2015 | 25830029 |
| role of pmk-1/p38 mapk defense in caenorhabditis elegans against klebsiella pneumoniae infection during host-pathogen interaction. | the present study reports that klebsiella pneumoniae (kp) killed the caenorhabditis elegans as a consequence of an accumulation and proliferation of the pathogen inside the worms' intestine. the real-time pcr analysis of the genes responsible for vulval development (let-23) and egg laying (lin-29) in kp infected c. elegans confirmed the reproductive defects provoked by kp at the molecular level. in addition, the genetic analysis in n2 wild type, tol-1, sek-1 and pmk-1 mutants unveiled that kp at ... | 2015 | 25819035 |
| carbapenem-resistant klebsiella pneumoniae meningitis: a case report. | | 2016 | 25817408 |
| carbapenemase-producing gram-negative bacteria: current epidemics, antimicrobial susceptibility and treatment options. | carbapenemases, with versatile hydrolytic capacity against β-lactams, are now an important cause of resistance of gram-negative bacteria. the genes encoding for the acquired carbapenemases are associated with a high potential for dissemination. in addition, infections due to gram-negative bacteria with acquired carbapenemase production would lead to high clinical mortality rates. of the acquired carbapenemases, klebsiella pneumoniae carbapenemase (ambler class a), verona integron-encoded metallo ... | 2015 | 25812463 |
| contemplating 3-hydroxypropionic acid biosynthesis in klebsiella pneumoniae. | 3-hydroxypropionic acid (3-hp) is a commercially valuable platform chemical from which an array of c3 compounds can be generated. klebsiella pneumoniae has been considered a promising species for biological production of 3-hp. despite a wealth of reports related to 3-hp biosynthesis in k. pneumoniae, its commercialization is still in infancy. the major hurdle hindering 3-hp overproduction lies in the poor understanding of glycerol dissimilation in k. pneumoniae. to surmount this problem, this re ... | 2015 | 25805899 |
| rapid induction of high-level carbapenem resistance in heteroresistant kpc-producing klebsiella pneumoniae. | enterobacteriaceae strains producing the klebsiella pneumoniae carbapenemase (kpc) have disseminated worldwide, causing an urgent threat to public health. kpc-producing strains often exhibit low-level carbapenem resistance, which may be missed by automated clinical detection systems. in this study, eight klebsiella pneumoniae strains with heterogeneous resistance to imipenem were used to elucidate the factors leading from imipenem susceptibility to high-level resistance as defined by clinical la ... | 2015 | 25801565 |
| presence of qaceδ1 and cepa genes and susceptibility to a hospital biocide in clinical isolates of klebsiella pneumoniae in iran. | the aim of this study was to evaluate the susceptibility of klebsiella pneumoniae clinical isolates to antibiotics and to a quaternary ammonium compound (qac) disinfectant as the concentrations used clinically and to determine the presence of the qaceδ1 and cepa genes for the first time in iran. in total, 85 k. pneumoniae isolates were randomly collected from hospitalized patients at the general hospitals in lorestan, iran. antibiotic and antiseptic susceptibility testing was performed according ... | 2015 | 25801259 |
| prevalence of ctx-m-8 and ctx-m-15 type extended-spectrum beta-lactamases between klebsiella pneumoniae spp. isolated from zahedan, southeast iran. | | 2016 | 25801185 |
| outbreak of multidrug resistant ndm-1-producing klebsiella pneumoniae from a neonatal unit in shandong province, china. | despite worldwide dissemination of new delhi metallo-β-lactamase1 (blandm-1), outbreaks remain uncommon in china. in this study, we describe the characteristics of the outbreak-related blandm-1-producing k. pneumonia isolates in a neonatal unit in shandong province, china. we recovered 21 non-repetitive carbapenem-resistant k. pneumoniae isolates with a positively modified hodge test (mht) or edta synergistic test from patients and environmental samples in shandong provincial hospital. pulsed-fi ... | 2015 | 25799421 |
| burden of bacterial meningitis: a retrospective review on laboratory parameters and factors associated with death in meningitis, kelantan malaysia. | to describe the clinical characteristics and the risk factors associated with mortality in patients with meningitis. this is a retrospective review of patients diagnosed to have meningitis with positive culture of the cerebrospinal fluid (csf) specimen. all cases aged 19 > years who were admitted to hospital usm between january 2004 and december 2011 were included in the study. the csf results database were obtained from the department of medical microbiology and parasitology, hospital usm, kela ... | 2015 | 25797971 |
| first report of a klebsiella pneumoniae st466 strain causing neonatal sepsis harbouring the blactx-m-15 gene in rabat, morocco. | klebsiella pneumoniae is one of the gram-negative bacilli most commonly found in urine of pregnant women and causing neonatal sepsis. the aim of this study was to analyse in terms of epidemiology and antimicrobial resistance of 23 k. pneumoniae isolates collected from vaginal swabs or urine of pregnant women, from pharyngeal and ear swabs of apparently healthy newborns and from peripheral cultures and hemocultures of newborns with suspected invasive neonatal infection in rabat, morocco. the prev ... | 2015 | 25790504 |
| correction: elucidation of the rama regulon in klebsiella pneumoniae reveals a role in lps regulation. | | 2015 | 25789473 |
| plasmid mediated antibiotic resistance in isolated bacteria from burned patients. | nowadays, the treatment of burned patients is difficult because of the high frequency of infection with antibiotic resistance bacteria. | 2015 | 25789121 |
| discovery of a cyclic boronic acid β-lactamase inhibitor (rpx7009) with utility vs class a serine carbapenemases. | the increasing dissemination of carbapenemases in gram-negative bacteria has threatened the clinical usefulness of the β-lactam class of antimicrobials. a program was initiated to discover a new series of serine β-lactamase inhibitors containing a boronic acid pharmacophore, with the goal of finding a potent inhibitor of serine carbapenemase enzymes that are currently compromising the utility of the carbapenem class of antibacterials. potential lead structures were screened in silico by modeling ... | 2015 | 25782055 |
| molecular characterization of extended spectrum beta-lactamases produced by klebsiella pneumoniae clinical strains from a tunisian hospital. | extended-spectrum β-lactamases are widespread in hospital settings worldwide. the prevalence of esbl producing klebsiella pneumoniae (esbl-kp) strains isolated from patients has recently increased in tunisia. we conducted this study to determine the prevalence and the genetic characterization of esbl-kp in a tunisian hospital. | 2015 | 25779010 |
| investigation on the cona binding properties of klebsiella pneumoniae. | klebsiella pneumoniae is a healthcare-associated bacterial pathogen which causes severe diseases in immunocompromised individuals. concanavalin a (cona), a lectin which recognizes proteins with mannose or glucose residues, has been reported to agglutinate k. pneumoniae and hence, is postulated to have therapeutical potential for k. pneumoniae-induced liver infection. this study investigated the cona binding properties of a large collection of clinical isolates of k. pneumoniae. cona agglutinatio ... | 2014 | 25776607 |
| prevalence of extended spectrum β-lactamase-producing klebsiella pneumoniae in clinical isolates. | extended spectrum β-lactamase (esbl) are gram-negative bacteria that produce the enzyme, β-lactamase, which can break down commonly used antibiotics, such as penicillin and cephalosporins, making infections with esbl producing bacteria more difficult to treat. extended spectrum β-lactamase-producing klebsiella pneumoniae were first reported in 1983 from germany, and since then a steady increase in resistance against cephalosporins has been seen causing health problems. | 2014 | 25774279 |
| platelet and endothelial cell p-selectin are required for host defense against klebsiella pneumoniae-induced pneumosepsis. | sepsis is associated with activation of platelets and endothelial cells accompanied by enhanced p-selectin surface expression. both platelet- and endothelial p-selectin have been associated with leukocyte recruitment and induction of inflammatory alterations. klebsiella (k.) pneumoniae is a common human sepsis pathogen, particularly in the context of pneumonia. | 2015 | 25773400 |
| hepatic abscess: diagnosis and management. | microbial contamination of the liver parenchyma leading to hepatic abscess (ha) can occur via the bile ducts or vessels (arterial or portal) or directly, by contiguity. infection is usually bacterial, sometimes parasitic, or very rarely fungal. in the western world, bacterial (pyogenic) ha is most prevalent; the mortality is high approaching 15%, due mostly to patient debilitation and persistence of the underlying cause. in south-east asia and africa, amebic infection is the most frequent cause. ... | 2015 | 25770745 |
| intestinal carriage of shewanella xiamenensis simulating carriage of oxa-48-producing enterobacteriaceae. | positivity for bla(oxa-48)-like carbapenemase genes was revealed by molecular testing of a surveillance rectal swab from a patient who had previously been colonized and infected by an oxa-48-producing klebsiella pneumoniae. positivity was due to a coincidental carriage of shewanella xiamenensis harboring a new bla(oxa-48)-like gene, while the k. pneumoniae was no longer present. | 2015 | 25766005 |
| detection of blaimp4 and blandm1 harboring klebsiella pneumoniae isolates in a university hospital in malaysia. | background : antibiotic resistance among enterobacteriaceae posts a great challenge to the health care service. the emergence of carbapenem-resistant klebsiella pneumoniae (crkp) is attracting significant attention due to its rapid and global dissemination. the infection is associated with significant morbidity and mortality, thus creating challenges for infection control and managing teams to curb the infection. in southeast asia, there have been limited reports and subsequent research regardin ... | 2015 | 25765342 |
| high-mobility group nucleosome-binding domain 2 protein inhibits the invasion of klebsiella pneumoniae into mouse lungs in vivo. | since bacterial invasion into host cells is a critical step in the infection process and the predominance of multiple-antibiotic-resistant klebsiella (k.) pneumoniae strains, using molecular agents to interfere with k. pneumoniae invasion is an attractive approach for the prevention of infection and suppress the immune inflammatory response. in previous studies by our group, high-mobility group nucleosome-binding domain 2 (hmgn2) protein was shown to exhibit anti-bacterial activity in vitro. the ... | 2015 | 25760831 |
| survey of metallo-β-lactamase-producing enterobacteriaceae colonizing patients in european icus and rehabilitation units, 2008-11. | the objective of this study was to perform a multinational survey of patients' colonization by metallo-β-lactamase (mbl)-producing enterobacteriaceae, including their molecular characterization. | 2015 | 25759034 |
| local acquisition and nosocomial transmission of klebsiella pneumoniae harbouring the blandm-1 gene in australia. | | 2015 | 25758700 |
| emergence of kpc-producing klebsiella pneumoniae st512 isolated from cerebrospinal fluid of a child in algeria. | we report class a carbapenemase (kpc)-3-producing klebsiella pneumoniae meningitis in a 6-month-old child in algeria. multilocus sequence typing showed that the sequence type obtained corresponded to st512, an allelic single-locus variant of the pandemic st258 widely distributed in kpc producers from europe. to our knowledge, this is the first report of kpc-3-producing k. pneumoniae st512 in a north african country. | 2015 | 25755890 |
| risk factors for infection with carbapenem-resistant klebsiella pneumoniae after liver transplantation: the importance of pre- and posttransplant colonization. | improved understanding of risk factors associated with carbapenem-resistant-klebsiella pneumoniae (cr-kp) infection after liver transplantation (lt) can aid development of effective preventive strategies. we performed a prospective cohort study of all adult patients undergoing lt at our hospital during 30-month period to define risk factors associated with cr-kp infection. all patients were screened for cr-kp carriage by rectal swabs before and after lt. no therapy was administered to decolonize ... | 2015 | 25754742 |
| genotypic characteristics of multidrug-resistant pseudomonas aeruginosa from hospital wastewater treatment plant in rio de janeiro, brazil. | to investigate pseudomonas aeruginosa isolates from a hospital wastewater treatment plant (hwtp), focusing on enzyme-based mechanisms of β-lactams resistance and the genetic relatedness among isolates. | 2015 | 25752460 |
| comparison of the clinical characteristics and outcomes of klebsiella pneumoniae and streptococcus pneumoniae meningitis. | this multicenter, retrospective cohort study compared the clinical characteristics and outcomes of community-acquired klebsiella pneumoniae meningitis (ca-kpm) with those of community-acquired streptococcus pneumoniae meningitis (ca-spm). eighty-three adult patients, 27 with ca-kpm and 56 with ca-spm, were included. diabetes mellitus (48.1% versus 21.4%; p=0.01) and liver cirrhosis (22.2% versus 5.4%; p=0.05) were more commonly associated with ca-kpm. comatose mental status (40.7% versus 12.5%; ... | 2015 | 25752203 |
| in silico analysis of usher encoding genes in klebsiella pneumoniae and characterization of their role in adhesion and colonization. | chaperone/usher (cu) assembly pathway is used by a wide range of enterobacteriaceae to assemble adhesive surface structures called pili or fimbriae that play a role in bacteria-host cell interactions. in silico analysis revealed that the genome of klebsiella pneumoniae lm21 harbors eight chromosomal cu loci belonging to γκп and ϭ clusters. of these, only two correspond to previously described operons, namely type 1 and type 3-encoding operons. isogenic usher deletion mutants of k. pneumoniae lm2 ... | 2015 | 25751658 |
| novel cord-like structures on mri in a case of hypervirulent klebsiella pneumoniae. | | 2015 | 25748749 |
| first case of ndm-1 producing klebsiella pneumoniae in caribbean islands. | characterize a ndm-1 producing k. pneumoniae isolate recovered from a patient hospitalized in guadeloupe, french west indies, after its transfer from cuba | 2015 | 25747780 |
| emerging issues in gram-negative bacterial resistance: an update for the practicing clinician. | the rapid and global spread of antimicrobial-resistant organisms in recent years has been unprecedented. although resistant gram-positive infections have been concerning to clinicians, the increasing incidence of antibiotic-resistant gram-negative infections has become the most pressing issue in bacterial resistance. indiscriminate antimicrobial use in humans and animals coupled with increased global connectivity facilitated the transmission of gram-negative infections harboring extended-spectru ... | 2015 | 25744116 |
| ndm-1-producing enterobacteriaceae in a teaching hospital in shanghai, china: incx3-type plasmids may contribute to the dissemination of blandm-1. | to provide the epidemiological dissemination and the genetic characteristics of bla(ndm-1) in a teaching hospital in shanghai, china. | 2015 | 25743762 |
| community-onset klebsiella pneumoniae pneumonia in taiwan: clinical features of the disease and associated microbiological characteristics of isolates from pneumonia and nasopharynx. | klebsiella pneumoniae is an important cause of community-onset pneumonia in asian countries and south africa. we investigated the clinical characteristics of k. pneumoniae causing community-onset pneumonia, and the associated microbiological features between k. pneumoniae isolates from pneumonia and those from the nasopharynx in taiwan. this study was conducted at the taipei veterans general hospital during july, 2012 to february, 2014. the clinical characteristics in patients with community-ons ... | 2015 | 25741336 |
| correlation of rapd-pcr profiles with esbl production in clinical isolates of klebsiella pneumoniae in tehran. | multidrug resistant k. pneumoniae, particularly the extended-spectrum β-lactamase (esbl) producing strains, are often responsible for the failure of antibiotic treatment in nosocomial infections. employing molecular methods to distinguish between esbl and non-esbl producing isolates can help quick identification of these multidrug resistant pathogens and thereby initiating appropriate antibiotic therapy. the aim of this study was to employ rapd-pcr to distinguish the genetic fingerprints of esbl ... | 2015 | 25737983 |
| tem and shv genes in klebsiella pneumoniae isolated from cockroaches and their antimicrobial resistance pattern. | klebsiella pneumoniae is a gram-negative rod bacterium, a known cause of community-acquired bacterial pneumonia and is an important hospital-acquired pathogen that causes severe morbidity and mortality. the aim of this study was to identify the tem and shv genes in k. pneumoniae isolated from cockroaches obtained from hospitals. | 2015 | 25737824 |
| alginate microparticles loaded with lipopolysaccharide subunit antigen for mucosal vaccination against klebsiella pneumoniae. | klebsiella pneumoniae (k. pneumoniae) is one of the commonest causes of nosocomial infections in human beings. since k. pneumoniae infections are air borne type, controlling it by mucosal vaccination through nasal and pulmonary route could be a promising approach in order to simulate the natural infection. new vaccines such as subunit vaccines are safer than traditional vaccines, but they are less immunogenic. therefore to enhance their immunogenicity, there is a need to develop potent and safe ... | 2015 | 25737397 |
| multidrug-resistant gram-negative bacteria: a product of globalization. | global trade and mobility of people has increased rapidly over the last 20 years. this has had profound consequences for the evolution and the movement of antibiotic resistance genes. there is increasing exposure of populations all around the world to resistant bacteria arising in the emerging economies. arguably the most important development of the last two decades in the field of antibiotic resistance is the emergence and spread of extended-spectrum β-lactamases (esbls) of the ctx-m group. a ... | 2015 | 25737092 |
| genotyping and characterization of ctx-m-15 -producing klebsiella pneumoniae isolated from an iranian hospital. | the aims were to describe the genetic characterization of blactx-m-1 group gene in klebsiella pneumoniae and to investigate the relationship between isolates by mlva and pfge. we analyzed 36 ctx-m group 1-esbl producing k. pneumoniae. rmpa and wcag virulence genes were identified by pcr. the genetic environment of blactx-m-1 was analyzed by pcr and sequencing. plasmid replicons were determined using pcr-based replicon typing. the isolates were typed by mlva and pfge. all blactx-m-1 were blactx-m ... | 2016 | 25734924 |
| tigecycline susceptibility and the role of efflux pumps in tigecycline resistance in kpc-producing klebsiella pneumoniae. | kpc-producing klebsiella pneumoniae isolates have emerged as important pathogens of nosocomial infections, and tigecycline is one of the antibiotics recommended for severe infections caused by kpc-producing k. pneumoniae. to identify the susceptibility profile of kpc-producing k. pneumoniae to tigecycline and investigate the role of efflux pumps in tigecycline resistance, a total of 215 kpc-producing k. pneumoniae isolates were collected. the minimum inhibitory concentration (mic) of tigecycline ... | 2015 | 25734903 |
| hypermucoviscous klebsiella syndrome without liver abscess in a patient with immunoglobulin g2 immune deficiency. | hypermucoviscous klebsiella pneumoniae (hmvkp) emerged as a cause of invasive infections in south-east (se) asia. it has become the most common cause of liver abscess in that region, and it is a significant causative organism in endogenous endophthalmitis and meningitis. during the past decade, cases of this uniquely virulent organism have been reported outside of se asia, with a propensity to affect individuals of se asian descent. cases have been reported from north america including canada. | 2014 | 25734148 |
| hypervirulent klebsiella pneumoniae. | hypervirulent strains of klebsiella pneumoniae are associated with abscess formation, commonly hepatic, and metastatic spread, even in healthy patients. we describe a case of this clinical syndrome, genotypic and phenotypic features of the isolate, and briefly review epidemiology, clinical manifestations, and pathogenesis of this underappreciated syndrome. | 2014 | 25734101 |
| positive autoregulation of mrkhi by the cyclic di-gmp-dependent mrkh protein in the biofilm regulatory circuit of klebsiella pneumoniae. | klebsiella pneumoniae is an important cause of nosocomial infections, primarily through the formation of surface-associated biofilms to promote microbial colonization on host tissues. expression of type 3 fimbriae by k. pneumoniae facilitates surface adherence, a process strongly activated by the cyclic di-gmp (c-di-gmp)-dependent transcriptional activator mrkh. in this study, we demonstrated the critical importance of mrkh in facilitating k. pneumoniae attachment on a variety of medically relev ... | 2015 | 25733612 |
| third-generation-cephalosporin-resistant klebsiella pneumoniae isolates from humans and companion animals in switzerland: spread of a dha-producing sequence type 11 clone in a veterinary setting. | characterization of third-generation-cephalosporin-resistant klebsiella pneumoniae isolates originating mainly from one human hospital (n = 22) and one companion animal hospital (n = 25) in bern (switzerland) revealed the absence of epidemiological links between human and animal isolates. human infections were not associated with the spread of any specific clone, while the majority of animal infections were due to k. pneumoniae sequence type 11 isolates producing plasmidic dha ampc. this clonal ... | 2015 | 25733505 |
| heteroresistance to colistin in klebsiella pneumoniae associated with alterations in the phopq regulatory system. | a multidrug-resistant klebsiella pneumoniae isolate exhibiting heteroresistance to colistin was investigated. the colistin-resistant subpopulation harbored a single amino acid change (asp191tyr) in protein phop, which is part of the phopq two-component system that activates pmrhfijklm expression responsible for l-aminoarabinose synthesis and polymyxin resistance. complementation assays with a wild-type phop gene restored full susceptibility to colistin. then, analysis of the colistin-susceptible ... | 2015 | 25733503 |
| evaluation of the nanochip® infection control panel test for direct detection and screening of methicillin-resistant staphylococcus aureus (mrsa), klebsiella pneumoniae carbapenemase (kpc)-producing bacteria and vancomycin-resistant enterococcus (vre). | rapid detection of infection control targets is needed and several bacterial target assays are commercially available. detection of patients colonized with klebsiella pneumoniae carbapenemase-producing enterobacteriaceae (kpc-cre), methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus (vre) comprises an essential part of infection control programs. this study evaluated the performance and feasibility of a novel molecular-based diagnostic screening test, the nan ... | 2015 | 25725804 |
| characterisation of extended-spectrum β-lactamases among klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in mozambique. | the aim of this study was to determine the prevalence of extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae isolated from urinary tract and bloodstream infections in a rural hospital in manhiça, mozambique. esbls were investigated among ceftriaxone-non-susceptible k. pneumoniae clinical isolates recovered between 2004 and 2009. characterisation of blactx-m, blashv, blaoxa and blatem genes was performed by pcr and sequencing. epidemiological relationships were established by phy ... | 2015 | 27873645 |
| atp exhibits antimicrobial action by inhibiting bacterial utilization of ferric ions. | atp up-regulates macrophage antimycobacterial activity in a p2x7-dependent manner, but little is known about whether atp directly exhibits antimicrobial effects against intracellular mycobacteria. in this study, we found that atp inhibited the growth of various bacteria, including staphylococcus, pseudomonas, and mycobacteria, without damaging bacterial surface structures. using gene technology, we newly established an enterobactin-deficient (entb(-)) mutant from atp-resistant klebsiella pneumon ... | 2015 | 25712807 |
| a high-resolution genomic analysis of multidrug-resistant hospital outbreaks of klebsiella pneumoniae. | multidrug-resistant (mdr) klebsiella pneumoniae has become a leading cause of nosocomial infections worldwide. despite its prominence, little is known about the genetic diversity of k. pneumoniae in resource-poor hospital settings. through whole-genome sequencing (wgs), we reconstructed an outbreak of mdr k. pneumoniae occurring on high-dependency wards in a hospital in kathmandu during 2012 with a case-fatality rate of 75%. the wgs analysis permitted the identification of two mdr k. pneumoniae ... | 2015 | 25712531 |
| a novel chemosynthetic peptide with β-sheet motif efficiently kills klebsiella pneumoniae in a mouse model. | klebsiella pneumoniae (kp) is one of the most common pathogens in nosocomial infections and is increasingly becoming multiple drug resistant. however, the molecular pathogenesis of kp in causing tissue injury and dysregulated host defense remains elusive, further dampening the development of novel therapeutic measures. we have previously screened a series of synthetic antimicrobial beta-sheet forming peptides and identified a peptide (irikirik; ie, ik8l) with a broad range of bactericidal activi ... | 2015 | 25709431 |
| risk factors for carbapenem-resistant klebsiella pneumoniae infection/colonization and predictors of mortality: a retrospective study. | to identify risk factors associated with carbapenem-resistant klebsiella pneumoniae (crkp) infection/colonization and death and to investigate the resistance and homology of crkp. | 2015 | 25707874 |
| plasmid-mediated antibiotic resistance and virulence in gram-negatives: the klebsiella pneumoniae paradigm. | plasmids harbor genes coding for specific functions including virulence factors and antibiotic resistance that permit bacteria to survive the hostile environment found in the host and resist treatment. together with other genetic elements such as integrons and transposons, and using a variety of mechanisms, plasmids participate in the dissemination of these traits resulting in the virtual elimination of barriers among different kinds of bacteria. in this article we review the current information ... | 2017 | 25705573 |
| [methods of phenotypic determination of new delhi metallo-beta-lactamase (ndm-1) in klebsiella penumoniae isolated in slovakia]. | reported is the first isolation and phenotypic determination of klebsiella pneumoniae producing new delhi metallo-beta-lactamase (ndm-1) isolated from patients in the slovak republic. | 2014 | 25702288 |
| in vitro emergence of carbapenem resistance in extended-spectrum β-lactamase-producing klebsiella pneumoniae clinical isolates. | | 2015 | 25699499 |
| performance of different culture methods and of a commercial molecular assay for the detection of carbapenemase-producing enterobacteriaceae in nursing homes and rehabilitation centers. | over the last several years, carbapenemase-producing enterobacteriaceae (cpe) have been increasingly detected not only among patients in acute care hospitals, but also in long-term care facilities. in this point prevalence survey, residents from three nursing homes and patients in one rehabilitation center were screened for asymptomatic intestinal carriage of cpe by rectal swabs. the first objective was to evaluate the hypothesis of the establishment of a cpe reservoir in a geriatric/chronic car ... | 2015 | 25698310 |
| characteristics and outcomes of klebsiella pneumoniae bacteraemia in hong kong. | we compared clinical outcomes between patients with healthcare-associated and community-acquired klebsiella pneumoniae bacteraemia and identified predictors associated with mortality and high treatment cost in hong kong. | 2015 | 25697339 |
| septicemia and infection due to esbl-producing k. pneumoniae following feline renal allograft transplantation. | a 12 yr old castrated male domestic longhair underwent renal transplantation for treatment of chronic interstitial nephritis. full-thickness intestinal biopsies obtained prior to transplantation revealed mild enteritis. twelve months following transplantation, the patient underwent surgery for resection of a mesenteric mass causing septic peritonitis. the mesenteric mass was resected and an intestinal resection and anastomosis was performed. extended-spectrum-β-lactamase producing klebsiella pne ... | 2015 | 25695559 |
| [utility of pneumonia severity index in hospitalized patients with pneumonia in intensive respiratory care units]. | the aim of this study was to evaluate the prognostic value of pneumonia severity index (psi) for prediction of 30-day mortality in patients admitted to intensive care unit (icu) for community-acquired pneumonia (cap). in patients with cap, comorbidities, complications, and physical, laboratory, radiological and microbiological findings were evaluated relative to their prognosis. | 2015 | 25693612 |