| hypervirulent klebsiella pneumoniae clones causing bacteraemia in adults in a teaching hospital in barcelona, spain (2007-2013). | virulent hypermucoviscous klebsiella pneumoniae strains associated with the maga and rmpa genes have mainly emerged in asia. we analysed the frequency and the clinical and molecular epidemiology of k. pneumoniae bacteraemia isolates obtained over a 7-year period (2007-2013). fifty-three of 878 k. pneumoniae invasive isolates (5.4%) showed a hypermucoviscous phenotype (by the string test). of these, 16 (30.2%) were maga(+)/rmpa(+), 12 (22.6%) were maga(-)/rmpa(+), and the remaining 25 (47.2%) wer ... | 2016 | 26454059 |
| first outbreak of oxa-48-positive carbapenem-resistant klebsiella pneumoniae isolates in constantine, algeria. | | 2015 | 26453148 |
| emergence of st147 klebsiella pneumoniae producing oxa-204 carbapenemase in a university hospital, tunisia. | molecular features of the first carbapenem-resistant klebsiella pneumoniae isolates (kp1 and kp2) from the university hospital tahar sfar, tunisia, were investigated. antimicrobial susceptibility testing, multilocus sequence typing, s1 nuclease pulsed-field gel electrophoresis, southern blot, and polymerase chain reaction (pcr)-based replicon typing were performed. extended-spectrum β-lactamases and carbapenemase genes were detected by pcr and sequencing. both isolates were multidrug resistant. ... | 2016 | 26447939 |
| impact of regional vein thrombosis in patients with klebsiella pneumoniae liver abscess. | klebsiella liver abscess (kla) is an emerging infection in asia caused by hypermucoviscous strains of klebsiella pneumoniae. it is associated with thrombophlebitis of portal and hepatic veins. the natural history and role of anticoagulation for this regional thrombophlebitis is unclear. in a retrospective study of 169 subjects with kla over 7 years, thrombophlebitis was identified in 53/169 (31.4%). only 1 received therapeutic anticoagulation. despite this 30/49 (73.2%) of those with follow up s ... | 2015 | 26444702 |
| septic pulmonary emboli associated with klebsiella pneumoniae epididymitis. | | 2016 | 26443643 |
| epidemiology of community-onset bloodstream infections in bouaké, central côte d'ivoire. | bacterial bloodstream infections (bsi) account for considerable morbidity worldwide, but epidemiological data from resource-constrained tropical settings are scarce. we analysed 293 blood cultures from patients presenting to a regional referral hospital in bouaké, central côte d'ivoire, to determine the aetiology of community-onset bsi. the prevalence of bacteraemia was 22.5%, with children being most commonly affected. enterobacteriaceae (predominantly klebsiella pneumoniae and salmonella enter ... | 2015 | 26442153 |
| prospectively defined murine mesenchymal stem cells inhibit klebsiella pneumoniae-induced acute lung injury and improve pneumonia survival. | numerous studies have described the immunosuppressive capacity of mesenchymal stem cells (msc) but these studies use mixtures of heterogeneous progenitor cells for in vitro expansion. recently, multipotent msc have been prospectively identified in murine bone marrow (bm) on the basis of pdfgra(+) sca1(+) cd45(-) ter119(-) (pαs) expression but the immunomodulatory capacity of these msc is unknown. | 2015 | 26438075 |
| hepatic metastasis complicated by abscess formation. | hepatic abscesses and hepatic metastasis are common diseases. however, hepatic abscesses seldom occur in patients with hepatic metastases. we describe a case of a 67-year-old female patient with abdominal pain in the right upper quadrant. magnetic resonance imaging revealed several lesions, with the largest lesion displaying features of both hepatic pyogenic abscess and liver metastasis. these features included iso- or hypointense signaling on t1wi and t2wi, hyperintense signaling on diffusion w ... | 2017 | 26430451 |
| complete genome sequence of klebsiella pneumoniae carbapenemase-producing k. pneumoniae myophage miro. | klebsiella pneumoniae is a gram-negative pathogen frequently associated with antibiotic-resistant nosocomial infections. bacteriophage therapy against k. pneumoniae may be possible to combat these infections. the following describes the complete genome sequence and key features of the pseudo-t-even k. pneumoniae carbapenemase (kpc)-producing k. pneumoniae myophage miro. | 2015 | 26430050 |
| complete genome sequence of carbapenemase-producing klebsiella pneumoniae myophage matisse. | klebsiella pneumoniae is a leading cause of nosocomial infections in the united states. due to the emergence of multidrug-resistant strains, phages targeting k. pneumoniae may be a useful alternative against this pathogen. here, we announce the complete genome of k. pneumoniae pseudo-t-even myophage matisse and describe its features. | 2015 | 26430049 |
| dna adenine methylation modulates pathogenicity of klebsiella pneumoniae genotype k1. | klebsiella pneumoniae genotype k1 is a highly virulent pathogen that causes liver abscess and metastatic endophthalmitis/meningitis. whether its pathogenicity is controlled by dna adenine methylase (dam), an epigenetic regulator of bacterial virulence gene expression, is yet unknown. we aimed to study the role of dna adenine methylation in the pathogenicity of k. pneumoniae genotype k1. | 2015 | 26427879 |
| klebsiella pneumoniae liver abscess complicated by endogenous endophthalmitis: the importance of early diagnosis and intervention. | | 2015 | 26424068 |
| ceftazidime-avibactam for the treatment of complicated urinary tract infections and complicated intra-abdominal infections. | treatment of complicated urinary tract infections and complicated intra-abdominal infections is increasingly difficult due to the rising prevalence of multidrug-resistant gram-negative bacteria. ceftazidime-avibactam is a combination of the established third-generation cephalosporin ceftazidime with avibactam, a novel non-β-lactam β-lactamase inhibitor, which restores the activity of ceftazidime against many β-lactamase-producing gram-negative bacteria, including extended-spectrum β-lactamases a ... | 2015 | 26420166 |
| [risk factors for multidrug-resistant klebsiella pneumoniae sepsis in children]. | to explore the risk factors for sepsis caused by multidrug-resistant klebsiella pneumonia (mdr-kp) and to provide a reference for the prevention of mdr-kp sepsis and rational use of antibiotics. | 2015 | 26412173 |
| dissemination of extensively drug-resistant and kpc-2 producing klebsiella pneumoniae isolated from bloodstream infections. | bloodstream infections (bsis) are serious diseases associated with high mortality, especially when caused by extensively drug-resistant (xdr) klebsiella pneumoniae. the prevalence and pandemic strains of extended-spectrum beta-lactamase (esbl)-producing k. pneumoniae isolated from blood cultures of patients with bsis were determined at sir run run shaw hospital, china. | 2015 | 26409744 |
| bactericidal and synergistic activity of double-carbapenem regimen for infections caused by carbapenemase-producing klebsiella pneumoniae. | available therapeutic options against carbapenem-resistant klebsiella pneumoniae (cr-kp) are limited because of the high level of resistance to other antimicrobial classes including polymyxins. the double-carbapenem regimen has been recently considered a possible therapeutic strategy. in the present study, we evaluated the in vitro bactericidal and synergistic activity of a double-carbapenem regimen consisting of ertapenem plus high-dose meropenem in a series of patients with healthcare-associat ... | 2016 | 26409059 |
| systematic review of antibiotic resistance rates among gram-negative bacteria in children with sepsis in resource-limited countries. | gram-negative antimicrobial resistance (amr) is of global concern, yet there are few reports from low- and low-middle-income countries, where antimicrobial choices are often limited. | 2015 | 26407352 |
| interspecific diversity reduces and functionally substitutes for intraspecific variation in biofilm communities. | diversity has a key role in the dynamics and resilience of communities and both interspecific (species) and intraspecific (genotypic) diversity can have important effects on community structure and function. however, a critical and unresolved question for understanding the ecology of a community is to what extent these two levels of diversity are functionally substitutable? here we show, for a mixed-species biofilm community composed of pseudomonas aeruginosa, p. protegens and klebsiella pneumon ... | 2016 | 26405829 |
| the complex epidemiology of carbapenem-resistant enterobacter infections: a multicenter descriptive analysis. | the pandemic of carbapenem-resistant enterobacteriaceae (cre) was primarily due to clonal spread of bla kpc producing klebsiella pneumoniae. thus, thoroughly studied cre cohorts have consisted mostly of k. pneumoniae. | 2015 | 26399907 |
| klebsiella pneumonia, a microorganism that approves the non-linear responses to antibiotics and window theory after exposure to wi-fi 2.4 ghz electromagnetic radiofrequency radiation. | drug resistance is widely believed to be an increasingly serious threat to global public health. we have previously reported that short term exposure of microorganisms to diagnostic ultrasound waves could significantly alter their sensitivity to antibiotics. in our previous studies, klebsiella pneumoniae showed major differences in the sensitivity to antibiotics in exposed and non-exposed samples. this study was aimed at investigating the alteration of antibiotic resistance of klebsiella pneumon ... | 2015 | 26396967 |
| incidence of colonization and bloodstream infection with carbapenem-resistant enterobacteriaceae in children receiving antineoplastic chemotherapy in italy. | few data are available on the incidence of carbapenemase-producing enterobacteriaceae (cpe) infection or colonization in children receiving anticancer chemotherapy. we performed a nationwide survey among centers participating in the pediatric hematology-oncology cooperative study group (associazione italiana ematologia oncologia pediatrica, aieop). during a 2-year observation period, we observed a threefold increase in the colonization rate, and a fourfold increase of bloodstream infection episo ... | 2016 | 26393496 |
| oral administration of gentamicin for prophylaxis of kpc-producing klebsiella pneumoniae gut colonization in patients treated with a novel parenchymal-sparing liver surgery: the gen gut study. | | 2016 | 26392255 |
| in vitro activity of the next-generation aminoglycoside plazomicin alone and in combination with colistin, meropenem, fosfomycin or tigecycline against carbapenemase-producing enterobacteriaceae strains. | carbapenemase-producing enterobacteriaceae (cpe) cause serious infections and are associated with high mortality in part due to limited treatment options. the in vitro activities of the new aminoglycoside plazomicin and comparators were evaluated against a collection of 164 cpe (vim-1, n=125; kpc-2, n=34; oxa-48, n=4; and imp-22, n=1). mic90 values of gentamicin, tobramycin and amikacin were 256, 64 and 16 mg/l, respectively. plazomicin exhibited an mic range of 0.12-4 mg/l with mic50 and mic90 ... | 2015 | 26391381 |
| electrochemical dna biosensor based on a glassy carbon electrode modified with gold nanoparticles and graphene for sensitive determination of klebsiella pneumoniae carbapenemase. | we describe the fabrication of a sensitive electrochemical dna biosensor for determination of klebsiella pneumoniae carbapenemase (kpc). the highly sensitive and selective electrochemical biosensor for dna detection was constructed based on a glassy carbon electrode (gce) modified with gold nanoparticles (au-nps) and graphene (gr). then au-nps/gr/gce was characterized by scanning electro microscope (sem), cyclic voltammetry (cv) and electrochemical impedance spectroscopy (eis). the hybridization ... | 2015 | 26387446 |
| successful treatment of carbapenemase-producing pandrug-resistant klebsiella pneumoniae bacteremia. | new antibiotic options are urgently needed for the treatment of carbapenem-resistant enterobacteriaceae infections. we report a 64-year-old female with prolonged hospitalization following an intestinal transplant who developed refractory bacteremia due to a serine carbapenemase-producing pandrug-resistant isolate of klebsiella pneumoniae. after failing multiple antimicrobial regimens, the patient was successfully treated. | 2015 | 26386029 |
| pecs regulates the urate-responsive expression of type 1 fimbriae in klebsiella pneumoniae cg43. | in the klebsiella pneumoniae cg43 genome, the divergently transcribed genes coding for pecs, the marr-type transcription factor, and pecm, the drug metabolite transporter, are located between the type 1 and type 3 fimbrial gene clusters. the intergenic sequence peco between pecs and pecm contains three putative pecs binding sites and a cpxr box. electrophoretic mobility shift assay revealed that the recombinant pecs and cpxr could specifically bind to the peco sequence, and the specific interact ... | 2015 | 26385366 |
| alert for klebsiella pneumoniae septicaemia in piglets. | | 2015 | 26385145 |
| emergence of carbapenemase producing klebsiella pneumonia and spread of kpc-2 and kpc-17 in taiwan: a nationwide study from 2011 to 2013. | the emergence of carbapenemase-producing klebsiella pneumoniae (cpkp) has become a great concern worldwide. in this study, 994 non-duplicate, carbapenem non-susceptible klebsiella pneumonia isolates were collected in taiwan from 2011 to 2013 for detection of the carbapenemase genes, assessment of antimicrobial susceptibility and molecular epidemiology studies. of these 994 isolates, 183 (18.4%) had carbapenemase genes: 157 (15.8%) kpc (145 kpc-2 and 12 kpc-17), 16 (1.6%) imp-8, 9 (0.9%) vim-1, a ... | 2015 | 26384242 |
| derepression of mineral phosphate solubilization phenotype by insertional inactivation of iclr in klebsiella pneumoniae. | the mode of succinate mediated repression of mineral phosphate solubilization and the role of repressor in suppressing phosphate solubilization phenotype of two free-living nitrogen fixing klebsiella pneumoniae strains was studied. organic acid mediated mineral phosphate solubilization phenotype of oxalic acid producing klebsiella pneumoniae sm6 and sm11 were transcriptionally repressed by iclr in presence of succinate as carbon source. oxalic acid production and expression of genes of the glyox ... | 2015 | 26381651 |
| carbapenemase-producing klebsiella pneumoniae with two different gene expression patterns. | | 2015 | 26372115 |
| biochemical characterization of vim-39, a vim-1-like metallo-β-lactamase variant from a multidrug-resistant klebsiella pneumoniae isolate from greece. | vim-39, a vim-1-like metallo-β-lactamase variant (vim-1 thr33ala his224leu) was identified in a clinical isolate of klebsiella pneumoniae belonging to sequence type 147. vim-39 hydrolyzed ampicillin, cephalothin, and imipenem more efficiently than did vim-1 and vim-26 (a vim-1 variant with the his224leu substitution) because of higher turnover rates. | 2015 | 26369975 |
| outbreaks of extended spectrum beta-lactamase-producing enterobacteriaceae in neonatal intensive care units: a systematic review. | to establish the number of outbreaks of extended spectrum beta-lactamase (esbl) producing organisms in neonatal intensive care units (nicus), to determine causes, mortality rates, proportions of infants colonised and infected and the interventions that terminated outbreaks. | 2016 | 26369370 |
| clinical features of non-clostridial gas gangrene and risk factors for in-hospital mortality. | to examine the clinical features of patients with non-clostridial gas gangrene (ncgg) at our hospital and identify risk factors for in-hospital mortality. | 2015 | 26369267 |
| endoplasmic reticulum chaperone gp96 in macrophages is essential for protective immunity during gram-negative pneumonia. | klebsiella pneumoniae is among the most common gram-negative bacteria that cause pneumonia. gp96 is an endoplasmic reticulum chaperone that is essential for the trafficking and function of toll-like receptors (tlrs) and integrins. to determine the role of gp96 in myeloid cells in host defence during klebsiella pneumonia, mice homozygous for the conditional hsp90b1 allele encoding gp96 were crossed with mice expressing cre-recombinase under control of the lysm promoter to generate lysmcre-hsp90b1 ... | 2016 | 26365983 |
| evaluation of antibiotic efficacy against infections caused by planktonic or biofilm cultures of pseudomonas aeruginosa and klebsiella pneumoniae in galleria mellonella. | the lack of novel antibiotics for more than a decade has placed increased pressure on existing therapies to combat the emergence of multidrug-resistant (mdr) bacterial pathogens. this study evaluated the galleria mellonella insect model in determining the efficacy of available antibiotics against planktonic and biofilm infections of mdr pseudomonas aeruginosa and klebsiella pneumoniae strains in comparison with in vitro minimum inhibitory concentration (mic) determination. in general, in vitro a ... | 2015 | 26364845 |
| negative pressure wound treatment of infections caused by extensively drug-resistant gram-negative bacteria after liver transplantation: two case reports. | although survival after liver transplantation (lt) has progressively improved over the last years, an increased prevalence of clinically relevant infections in lt patients is well documented. in particular, the spread of infections sustained by extensively drug-resistant bacteria (xdr) produced an increase in the incidence of wound infections. implementation of treatments for these life-threatening events is mandatory. this study describes 2 lt patients in whom xdr wound infection was effectivel ... | 2015 | 26361664 |
| evaluation of polymyxin susceptibility profile among kpc-producing klebsiella pneumoniae using etest and microscan walkaway automated system. | determination of polymyxin susceptibility profile is important to monitor resistance rates and for implementing control measures for polymyxin-resistant carbapenem-resistant enterobacteriaceae. some laboratorial methods have been used to determine the polymyxin susceptibility profile. however, the performance of microscan walkaway has been poorly reported for kpc-producing klebsiella pneumoniae, so far. to evaluate two different methods, etest and the microscan automated system, in determining m ... | 2015 | 26361367 |
| distribution of β-lactamase genes among carbapenem-resistant klebsiella pneumoniae strains isolated from patients in turkey. | the emergence of carbapenem-resistant klebsiella pneumoniae poses a serious problem to antibiotic management. we investigated the β-lactamases in a group of carbapenem-resistant k. pneumoniae clinical isolates from turkey. | 2015 | 26354347 |
| detection of the klebsiella pneumoniae carbapenemase (kpc) in k. pneumoniae isolated from the clinical samples by the phenotypic and genotypic methods. | the production of carbapenemases especially klebsiella pneumoniae carbapenemase (kpc) is the most important mechanism of enzymatic resistance in isolated enterobacteriaceae such as k. pneumoniae . the purpose of this study was detected of the carbapenemase producer k. pneumoniae strains with phenotypic and genotypic methods. | 2015 | 26351485 |
| interactions of klebsiella pneumoniae with the innate immune system vary in relation to clone and resistance phenotype. | apart from inadequate antimicrobial treatment, specific virulence factors contribute to the high attributable mortality of infections caused by multidrug-resistant (mdr) klebsiella pneumoniae. we explored the roles of mdr and clones as virulence determinants of k. pneumoniae and their interaction with innate immunity. twenty isolates were studied and characterized by resistance phenotype and multilocus sequence type (mlst). human peripheral blood mononuclear cells (pbmcs) were stimulated for the ... | 2015 | 26349819 |
| nlrp12 modulates host defense through il-17a-cxcl1 axis. | we used an extracellular pathogen klebsiella pneumoniae to determine the role of nlrp12 (nod-like receptor (nlr) family pyrin domain containing 12) as this bacterium is associated with devastating pulmonary infections. we found that human myeloid cells (neutrophils and macrophages) and non-myeloid cells (epithelial cells) show upregulation of nlrp12 in human pneumonic lungs. nlrp12-silenced human macrophages and murine nlrp12(-/-) macrophages displayed reduced activation of nuclear factor-κb and ... | 2016 | 26349659 |
| complete sequences of kpc-2-encoding plasmid p628-kpc and ctx-m-55-encoding p628-ctxm coexisted in klebsiella pneumoniae. | a carbapenem-resistant klebsiella pneumoniae strain 628 was isolated from a human case of intracranial infection in a chinese teaching hospital. strain 628 produces kpc-2 and ctx-m-55 encoded by two different conjugative plasmids, i.e., the incfiik plasmid p628-kpc and the inci1 plasmid p628-ctxm respectively. bla kpc-2 is captured by a tn1722-based unit transposon with a linear structure. δtn3-iskpn27-bla kpc-2-δiskpn6-δtn1722 and this transposon together with a mercury resistance (mer) gene lo ... | 2015 | 26347725 |
| klebsiella pneumoniae fimk promotes virulence in murine pneumonia. | klebsiella pneumoniae, a chief cause of nosocomial pneumonia, is a versatile and commonly multidrug-resistant human pathogen for which further insight into pathogenesis is needed. we show that the pilus regulatory gene fimk promotes the virulence of k. pneumoniae strain top52 in murine pneumonia. this contrasts with the attenuating effect of fimk on urinary tract virulence, illustrating that a single factor may exert opposing effects on pathogenesis in distinct host niches. loss of fimk in top52 ... | 2016 | 26347570 |
| metabolic engineering of klebsiella pneumoniae for the de novo production of 2-butanol as a potential biofuel. | butanol isomers are important bulk chemicals and promising fuel substitutes. the inevitable toxicity of n-butanol and isobutanol to microbial cells hinders their final titers. in this study, we attempt to engineer klebsiella pneumoniae for the de novo production of 2-butanol, another butanol isomer which shows lower toxicity than n-butanol and isobutanol. 2-butanol synthesis was realized by the extension of the native meso-2,3-butanediol synthesis pathway with the introduction of diol dehydratas ... | 2015 | 26342337 |
| epidemiology and outcomes of carbapenem-resistant klebsiella pneumoniae bacteriuria in kidney transplant recipients. | little is known about the epidemiology of carbapenem-resistant klebsiella pneumoniae (crkp) bacteriuria following kidney transplantation. we determined the incidence of post-transplant crkp bacteriuria in adults who underwent kidney transplant from 2007 to 2010 at 2 new york city centers. | 2015 | 26341757 |
| dissemination of multidrug-resistant blactx-m-15/incfiik plasmids in klebsiella pneumoniae isolates from hospital- and community-acquired human infections in tunisia. | this study investigated the molecular features of extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae from hospital- and community-acquired (ha/ca) infections in the region of mahdia, tunisia. among 336 k. pneumoniae isolates recovered from both clinical contexts between july 2009 and december 2011, 49 and 15 were esbl producers and originated from clinical and community sources, respectively. all isolates produced the ctx-m-15 enzyme. as shown by southern blot on s1 nuclease tr ... | 2015 | 26341707 |
| inhibition of klebsiella β-lactamases (shv-1 and kpc-2) by avibactam: a structural study. | β-lactamase inhibition is an important clinical strategy in overcoming β-lactamase-mediated resistance to β-lactam antibiotics in gram negative bacteria. a new β-lactamase inhibitor, avibactam, is entering the clinical arena and promising to be a major step forward in our antibiotic armamentarium. avibactam has remarkable broad-spectrum activity in being able to inhibit classes a, c, and some class d β-lactamases. we present here structural investigations into class a β-lactamase inhibition by a ... | 2015 | 26340563 |
| complete genome sequence of klebsiella pneumoniae carbapenemase-producing k. pneumoniae siphophage sushi. | klebsiella pneumoniae is a gram-negative bacterium in the family enterobacteriaceae. it is associated with numerous nosocomial infections, including respiratory and urinary tract infections in humans. the following reports the complete genome sequence of k. pneumoniae carbapenemase-producing k. pneumoniae t1-like siphophage sushi and describes its major features. | 2015 | 26337889 |
| risk factors and outcomes of carbapenem-resistant klebsiella pneumoniae infections in liver transplant recipients. | | 2016 | 26335447 |
| investigation of clonal relationships of k. pneumoniae isolates from neonatal intensive care units by pfge and rep-pcr. | clonal relationships of klebsiella pneumoniae strains obtained during an epidemic and after a one-year post-epidemic (non-epidemic) period in the same neonatal intensive care unit (nicu) using pulsed-field gel electrophoresis (pfge) and repetitive polymerase chain reaction (rep-pcr) by the diversilab (dl) system were investigated, and the results of both molecular techniques were evaluated. | 2015 | 26322874 |
| role of efflux pumps inhibitor in decreasing antibiotic resistance of klebsiella pneumoniae in a tertiary hospital in north india. | the contribution of efflux systems to drug resistance in enterobacteriaceae is becoming increasingly appreciated. this study phenotypically analyzed the role of efflux mechanisms in resistance to ertapenem, doripenem, and tigecycline among clinical isolates of carbapenem-resistant klebsiella pneumoniae (crkp). | 2015 | 26322872 |
| attributable mortality of carbapenem-resistant klebsiella pneumoniae infections in a prospective matched cohort study in italy, 2012-2013. | in italy, infections with carbapenem-resistant klebsiella pneumoniae (crkp) have increased markedly since 2009, creating unprecedented problems in healthcare settings and limiting treatment options for infected patients. | 2016 | 26319590 |
| pleural and pericardial effusions combined with ascites in a patient with severe sunitinib-induced hypothyroidism. | to best of our knowledge, this is the first reported case of pericardial and pleural effusions combined with ascites, precipitated with severe sunitinib-induced hypothyroidism. a 58-year-old man presented in our emergency department due to dyspnoea and dry cough. sixteen months earlier, the patient underwent left nephrectomy due to metastatic renal cell adenocarcinoma (rcc), and therapy with sunitinib was initiated postoperatively. thyroid function was not assessed during the therapy. on admissi ... | 2016 | 26319226 |
| risk factors for in-hospital mortality in patients with type 2 diabetes complicated by community-acquired klebsiella pneumoniae bacteremia. | patients with diabetes are at a high risk of infection-related morbidity and mortality. klebsiella pneumoniae bacilli are prevalent among diabetic patients, especially in asian populations. the present study aimed to identify risk factors for in-hospital mortality among diabetic patients complicated by community-acquired k. pneumoniae bacteremia. | 2015 | 26315482 |
| correlation of bacterial isolates from middle ear and nasopharynx in patients with chronic suppurative otitis media in ilorin, nigeria. | to determine the association between isolates in the middle ear (me) and nasopharynx of patients with chronic otitis media in ilorin, north-central nigeria. | 2015 | 28239265 |
| investigation of a possible outbreak of ndm-5-producing st16 klebsiella pneumoniae among patients in denmark with no history of recent travel using whole-genome sequencing. | | 2015 | 27873714 |
| efficacy of tigecycline-colistin combination in the treatment of carbapenem-resistant klebsiella pneumoniae endocarditis. | here we discuss the efficacy of colistin-tigecycline combination in the treatment of multidrug-resistant (mdr) klebsiella pneumoniae infective endocarditis (ie). we report a case of a 67-year-old head-injured patient who developed a carbapenem-resistant k. pneumoniae ie. the patient was treated with colistin-tigecycline combination, with a favourable outcome. in conclusion, colistin-tigecycline combination may be a possible combination in the therapy of ie caused by mdr enterobacteriaceae. | 2015 | 27873712 |
| antimicrobial resistance and resistance mechanisms of enterobacteriaceae in icu and non-icu wards in europe and north america: smart 2011-2013. | intensive care units (icus) are often described as hotbeds of antimicrobial resistance, with high rates of extended-spectrum β-lactamase (esbl)-producing and multidrug-resistant (mdr) enterobacteriaceae. data from the smart study were used to examine differences between the susceptibility of enterobacteriaceae from icu and non-icu wards in europe and north america. in total, 21,470 enterobacteriaceae isolates from intra-abdominal and urinary tract infections were collected at 90 sites in 20 euro ... | 2015 | 27873708 |
| diversity of the genetic environment of the blakpc-2 gene among klebsiella pneumoniae clinical isolates in a chinese hospital. | kpc-producing klebsiella pneumoniae (kpc-kp) has been frequently reported worldwide and constitutes a major healthcare threat, given their extensively drug-resistant phenotypes. in this study, we report the characterization of the genetic environment of blakpc-2 gene in kpc-kp clinical strains from china belonging to diverse genotypes. thirty-five nonduplicated kpc-kp isolates collected in a chinese hospital during 2012 were analyzed. all were multidrug resistant due to the presence of other res ... | 2016 | 26313117 |
| antimicrobial susceptibility pattern of extended-spectrum beta- lactamase producing klebsiella pneumoniae clinical isolates in an indian tertiary hospital. | there is an increased prevalence of extended-spectrum beta-lactamase producing klebsiella pneumoniae (esbl-kp) worldwide including india, which is a major concern for the clinicians, especially in intensive care units and pediatric patients. this study aims to determine the prevalence of esbl-kp and antimicrobial sensitivity profile to plan a proper hospital infection control program to prevent the spread of resistant strains. | 2017 | 26312255 |
| gradual increase in antibiotic concentration affects persistence of klebsiella pneumoniae. | sublethal bactericidal antibiotics promote the formation of multidrug-tolerant persisters. clinically, serum drug concentration increases gradually and reaches the peak level with high killing efficiency some time after administration. this study aimed to investigate if the initial low antibiotic concentration would promote persister formation in klebsiella pneumoniae, an increasingly important nosocomial pathogen. | 2015 | 26311842 |
| a common flanking region in promiscuous plasmids encoding blandm-1 in klebsiella pneumoniae isolated in singapore. | bacteria encoding the new delhi metallo-β-lactamase gene (blandm-1) are regarded as superbugs for their resistance to multiple antibiotics. plasmids encoding blandm-1 have been observed to be spreading among gram-negative bacteria around the world. previous studies have demonstrated that multiple modifications of blandm-1-harboring plasmids might contribute to the spread of the gene. in this study, we analyzed blandm-1-encoding plasmids from two klebsiella pneumoniae isolates, du7433 and du1301, ... | 2016 | 26308279 |
| emergence of a hypervirulent carbapenem-resistant klebsiella pneumoniae isolate from clinical infections in china. | hypervirulent klebsiella pneumoniae (hvkp) infections occur worldwide, but carbapenem-resistant hvkp strain has rarely been observed. | 2015 | 26304687 |
| clonal dissemination of multilocus sequence type st15 kpc-2-producing klebsiella pneumoniae in bulgaria. | a total of 36 consecutive clinical and two fecal-screening carbapenem-resistant klebsiella pneumoniae isolates from two bulgarian university hospitals (varna and pleven) were investigated. susceptibility testing, conjugation experiments, and plasmid replicon typing were carried out. beta-lactamases were characterized by isoelectric focusing, pcr, and sequencing. clonal relatedness was investigated by rapd and multilocus sequence typing (mlst). most of the isolates demonstrated multidrug resistan ... | 2015 | 26303718 |
| prevalence of plasmid-mediated quinolone resistance determinants and oqxab efflux pumps among extended-spectrum β-lactamase producing klebsiella pneumoniae isolated from patients with nosocomial urinary tract infection in tehran, iran. | objective. plasmid-mediated quinolone resistance (pmqr) plays an important role in the development of clinical resistance to quinolone. the aim of this study was to investigate pmqr determinants among extended-spectrum β-lactamases- (esbl-) producing klebsiella pneumoniae recovered from patients with nosocomial urinary tract infection (uti). methods. a total of 247 esbl-producing k. pneumoniae isolates were collected from 750 patients with uti. esbl production was confirmed by double disc synerg ... | 2015 | 26301114 |
| bloodstream infections in intensive care unit patients: distribution and antibiotic resistance of bacteria. | bloodstream infections (bsis) are among the leading infections in critically ill patients. the case-fatality rate associated with bsis in patients admitted to intensive care units (icus) reaches 35%-50%. the emergence and diffusion of bacteria with resistance to antibiotics is a global health problem. multidrug-resistant bacteria were detected in 50.7% of patients with bsis in a recently published international observational study, with methicillin resistance detected in 48% of staphylococcus au ... | 2015 | 26300651 |
| kidney transplant recipients infected with blakpc-2-producing klebsiella pneumoniae. | | 2015 | 26299180 |
| improved phenotype-based definition for identifying carbapenemase producers among carbapenem-resistant enterobacteriaceae. | preventing transmission of carbapenemase-producing, carbapenem-resistant enterobacteriaceae (cp-cre) is a public health priority. a phenotype-based definition that reliably identifies cp-cre while minimizing misclassification of non-cp-cre could help prevention efforts. to assess possible definitions, we evaluated enterobacterial isolates that had been tested and deemed nonsusceptible to >1 carbapenem at us emerging infections program sites. we determined the number of non-cp isolates that met ( ... | 2015 | 26290955 |
| efficacy of appropriate antimicrobial therapy on the survival of patients with carbapenem nonsusceptible klebsiella pneumoniae infection: a multicenter study in taiwan. | the impact of antimicrobial treatment on the outcome of carbapenem nonsusceptible klebsiella pneumoniae (cnskp) infections needs to be elucidated. this nationwide, multicenter study was conducted to evaluate the impact of appropriate antimicrobial therapy on 14-day mortality among patients with cnskp infection in taiwan.patients with cnskp infections from 11 medical centers and 4 regional hospitals in taiwan were enrolled in 2013. carbapenem nonsusceptibility was defined as a minimum inhibitory ... | 2015 | 26287432 |
| loss of hypermucoviscosity and increased fitness cost in colistin-resistant klebsiella pneumoniae sequence type 23 strains. | in this study, we investigated the effects of colistin resistance on virulence and fitness in hypermucoviscous (hv) klebsiella pneumoniae sequence type 23 (st23) strains. colistin-resistant mutants were developed from three colistin-susceptible hv k. pneumoniae st23 strains. the lipid a structures of strains were analyzed by matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry. changes in hv were investigated using the string test, and extracellular polysaccha ... | 2015 | 26282408 |
| risk factors for bloodstream infections due to colistin-resistant kpc-producing klebsiella pneumoniae: results from a multicenter case-control-control study. | the increasing prevalence of colistin resistance (colr) klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae (kp) is a matter of concern because of its unfavourable impact on mortality of kpc-kp bloodstream infections (bsi) and the shortage of alternative therapeutic options. a matched case-control-control analysis was conducted. the primary study end point was to assess risk factors for colr kpc-kp bsi. the secondary end point was to describe mortality and clinical characteristics ... | 2015 | 26278669 |
| kpc-2-producing klebsiella pneumoniae in a hospital in the midwest region of brazil. | the emergence of β-lactamase-producing enterobacteriaceae in the last few decades has become major challenge faced by hospitals. in this study, isolates of klebsiella pneumoniae carbapenemase-2 (kpc-2)-producing k. pneumoniae from a tertiary hospital in mato grosso do sul, brazil, were characterized. bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof; bruker daltonics, germany) mass spectrometry. the minimum inhibitory concentrations o ... | 2015 | 26273265 |
| complete genome sequence of phytopathogenic pectobacterium atrosepticum bacteriophage peat1. | pectobacterium atrosepticum is a common phytopathogen causing significant economic losses worldwide. to develop a biocontrol strategy for this blackleg pathogen of solanaceous plants, p. atrosepticum bacteriophage peat1 was isolated and its genome completely sequenced. interestingly, morphological and sequence analyses of the 45,633-bp genome revealed that phage peat1 is a member of the family podoviridae and most closely resembles the klebsiella pneumoniae bacteriophage kp34. this is the first ... | 2015 | 26272557 |
| pediatric emphysematous cystitis: a report and review of a rare diagnosis in children. | an 11-year-old female with spastic quadriplegia was seen in the emergency room with abdominal pain, vomiting and anorexia. labs revealed possible pancreatitis and signs of a urinary tract infection. a ct scan was performed to assess her abdominal pain and demonstrated circumferential air within the bladder wall. following cultures being drawn, she was started on broad spectrum antibiotics. her urine eventually grew klebsiella pneumoniae. follow up imaging 2 weeks later demonstrated resolution of ... | 2015 | 26267032 |
| two cases of klebsiella pneumoniae liver abscess necessitating liver resection for effective treatment. | we report two british cases of liver abscess, due to klebsiella pneumoniae and associated with synchronous infection elsewhere, which required liver resection for definitive treatment. they illustrate the geographic spread of aggressive k pneumoniae liver infection and demonstrate the importance of early aggressive treatment. | 2015 | 26263824 |
| new antibiotics and antimicrobial combination therapy for the treatment of gram-negative bacterial infections. | increasing rates of life-threatening infections due to multidrug-resistant (mdr) gram-negative bacteria, such as carbapenemase-producer strains, as well as pathogens that are resistant to all current therapeutic options, have been reported. the number of compounds that are currently being developed is still insufficient to control this global threat. we have reviewed the current available options for the treatment of mdr gram-negative infections, including combination regimens employing older an ... | 2015 | 26263298 |
| deep transcriptome profiling of clinical klebsiella pneumoniae isolates reveals strain and sequence type-specific adaptation. | health-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. in klebsiella pneumoniae, the dissemination of the k. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. although antibiotic resistanc ... | 2015 | 26261087 |
| first report of two rapid-onset fatal infections caused by a newly emerging hypervirulent k. pneumonia st86 strain of serotype k2 in china. | here, we present the first report of one suspected dead case and two confirmed rapid-onset fatal infections caused by a newly emerging hypervirulent klebsiella pneumoniae st86 strain of serotype k2. the three cases occurred in a surgery ward during 2013 in shanghai, china. a combination of multilocus sequence typing, pulsed-field gel electrophoresis, phenotypic and pcr tests for detecting virulence factors (vfs) was used to identify the isolates as k2 st86 strains with common vfs, including aero ... | 2015 | 26257712 |
| cefepime combined with amoxicillin/clavulanic acid: a new choice for the kpc-producing k. pneumoniae infection. | clinical treatment for blakpc-positive klebsiella pneumoniae isolates is challenging because the recommended antibiotic options are limited and are extraordinarily expensive. this study aimed to explore a new therapy for infection caused by kpc-producing k. pneumoniae. | 2015 | 26255892 |
| microbiologic characteristics and predictors of mortality in bloodstream infections in intensive care unit patients: a 1-year, large, prospective surveillance study in 5 italian hospitals. | bloodstream infections (bsis) from multidrug-resistant (mdr) bacteria cause morbidity and mortality in intensive care unit (icu) patients worldwide. this study investigated the incidence of bsis in 5 adult general icus in rome, italy, and evaluated the mortality rate and risk factors associated with these infections. | 2015 | 26253805 |
| klebsiella pneumoniae subsp. pneumoniae-bacteriophage combination from the caecal effluent of a healthy woman. | a sample of caecal effluent was obtained from a female patient who had undergone a routine colonoscopic examination. bacteria were isolated anaerobically from the sample, and screened against the remaining filtered caecal effluent in an attempt to isolate bacteriophages (phages). a lytic phage, named klpn1, was isolated on a strain identified as klebsiella pneumoniae subsp. pneumoniae (capsular type k2, rmpa (+)). this siphoviridae phage presents a rosette-like tail tip and exhibits depolymerase ... | 2015 | 26246963 |
| reparixin attenuates neuronal injury in experimental klebsiella pneumoniae meningoencephalitis through dual effects on neuroprotection and neuroinflammation. | bacterial meningitis causes high mortality and brain damage. the host immune response is associated with brain injury. chemokine (c-x-c motif) (cxc) chemokines are neutrophil chemoattractants. this study focused on the beneficial effects of intracerebroventricular administration of reparixin, an inhibitor of chemokine (c-x-c motif) receptor (cxcr)1/2, to rats at 2 h following experimental klebsiella pneumoniae meningoencephalitis. | 2016 | 26245311 |
| production of a modified peptide clavanin in pichia pastoris: cloning, expression, purification and in vitro activities. | antimicrobial peptides are one of the most promising peptide-based drugs due to their enormous potential as novel biopharmaceuticals in both human and animal industries. in order to develop strategies to over produce such molecules, heterologous production of a modified version of clavanin a, here named clavanin mo (clavmo), was successfully achieved in the methylothopic yeast pichia pastoris. clavmo was fused to thioredoxin as a carrier protein and the construction was tested using two promoter ... | 2015 | 26243173 |
| inactivation of the virulence factors from 2,3-butanediol-producing klebsiella pneumoniae. | the microbiological production of 2,3-butanediol (2,3-bdo) has attracted considerable attention as an alternative way to produce high-value chemicals from renewable sources. among the number of 2,3-bdo-producing microorganisms, klebsiella pneumoniae has been studied most extensively and is known to produce large quantity of 2,3-bdo from a range of substrates. on the other hand, the pathogenic characteristics of the bacteria have limited its industrial applications. in this study, two major virul ... | 2015 | 26239074 |
| intravenous fosfomycin treatment for carbapenem-resistant klebsiella pneumoniae in the united states. | | 2015 | 26238469 |
| allocation of klebsiella pneumoniae bloodstream isolates into four distinct groups by ompk36 typing in a taiwanese university hospital. | the ompk36 porin plays a role in carbapenem resistance and may contribute to bacterial virulence in klebsiella pneumoniae. this study aimed to investigate the characteristics of different groups of k. pneumoniae separated by ompk36 typing. among 226 nonduplicate k. pneumoniae bloodstream isolates collected at a taiwanese hospital in 2011, four ompk36 types, designated types a, b, c, and d, were identified by pcr in 61, 28, 100, and 36 isolates, respectively; 1 isolate was untypeable. statistical ... | 2015 | 26224840 |
| trends in susceptibility rates and extended-spectrum β-lactamase production of klebsiella pneumoniae in bloodstream infections across the united states veterans affairs healthcare system. | klebsiella pneumoniae is an important pathogen, increasingly notorious for its ability to become resistant to antimicrobial agents. this study sought to characterize trends in antimicrobial susceptibility rates for k. pneumoniae causing bacteremias across the united states (u.s.) veterans healthcare administration (vha) from 2007 through 2013 utilizing a national clinical database. k. pneumoniae grew in 9,235 blood cultures from 8,414 patients. nationally, ampicillin-sulbactam, ceftazidime, cefe ... | 2015 | 26207392 |
| genotypes of ciprofloxacin-resistant klebsiella pneumoniae in korea and their characteristics according to the genetic lineages. | we investigated the molecular genotypes of ciprofloxacin-resistant klebsiella pneumoniae and their characteristics according to the genetic lineages. for 160 k. pneumoniae collected in 2013, ciprofloxacin minimum inhibitory concentrations (mics) were determined by agar dilution method. the genotypes of ciprofloxacin-resistant k. pneumoniae isolates were determined by multilocus sequence typing (mlst) and wzi gene typing. the presence of plasmid-mediated resistance determinants [qnra, qnrb, qnrs, ... | 2015 | 26207318 |
| intermingled klebsiella pneumoniae populations between retail meats and human urinary tract infections. | klebsiella pneumoniae is a common colonizer of the gastrointestinal tract of humans, companion animals, and livestock. to better understand potential contributions of foodborne k. pneumoniae to human clinical infections, we compared k. pneumoniae isolates from retail meat products and human clinical specimens to assess their similarity based on antibiotic resistance, genetic relatedness, and virulence. | 2015 | 26206847 |
| editorial commentary: genomic epidemiology: revealing hidden reservoirs for klebsiella pneumoniae. | | 2015 | 26206846 |
| genome sequence of klebsiella pneumoniae yzusk-4, a bacterium proposed as a starter culture for fermented meat products. | klebsiella pneumoniae strain yzusk-4, isolated from chinese rugao ham, is an efficient branched-chain aminotransferase-producing bacterium that can be used widely in fermented meat products to enhance flavor. the draft genome sequence of strain yzusk-4 may provide useful genetic information on branched-chain amino acid aminotransferase production and branched-chain amino acid metabolism. | 2015 | 26205853 |
| molecular analysis of antibiotic resistance determinants and plasmids in malaysian isolates of multidrug resistant klebsiella pneumoniae. | infections caused by multidrug resistant klebsiella pneumoniae have been increasingly reported in many parts of the world. a total of 93 malaysian multidrug resistant k. pneumoniae isolated from patients attending to university of malaya medical center, kuala lumpur, malaysia from 2010-2012 were investigated for antibiotic resistance determinants including extended-spectrum beta-lactamases (esbls), aminoglycoside and trimethoprim/sulfamethoxazole resistance genes and plasmid replicons. ctx-m-15 ... | 2015 | 26203651 |
| complete genome sequence of endophytic nitrogen-fixing klebsiella variicola strain dx120e. | klebsiella variicola strain dx120e (=cgmcc 1.14935) is an endophytic nitrogen-fixing bacterium isolated from sugarcane crops grown in guangxi, china and promotes sugarcane growth. here we summarize the features of the strain dx120e and describe its complete genome sequence. the genome contains one circular chromosome and two plasmids, and contains 5,718,434 nucleotides with 57.1% gc content, 5,172 protein-coding genes, 25 rrna genes, 87 trna genes, 7 ncrna genes, 25 pseudo genes, and 2 crispr re ... | 2015 | 26203334 |
| large nosocomial outbreak of colistin-resistant, carbapenemase-producing klebsiella pneumoniae traced to clonal expansion of an mgrb deletion mutant. | we describe a large hospital outbreak (93 bloodstream infections) of colistin-resistant klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae isolates which was mirrored by increased colistin consumption. the outbreak was mostly traced to the clonal expansion of an mgrb deletion mutant of an st512 strain that produced kpc-3. | 2015 | 26202124 |
| outbreak of colistin-resistant, carbapenemase-producing klebsiella pneumoniae: are we at the end of the road? | carbapenem-resistant klebsiella pneumoniae strains that produce k. pneumoniae carbapenemase (kpc) have spread globally in the last decade. colistin is a key agent in treating infections caused by this pathogen. in this issue of the journal of clinical microbiology, giani et al. (t. giani, f. arena, g. vaggelli, v. conte, a chiarell, l. h. de angelis, r. fornaini, m. grazzini, f. niccolini, p. pecile, and g. m. rossolini, j clin microbiol 53:3341-3344, 2015, http://dx.doi.org/10.1128/jcm.01017-15 ... | 2015 | 26202122 |
| mapping the evolution of hypervirulent klebsiella pneumoniae. | highly invasive, community-acquired klebsiella pneumoniae infections have recently emerged, resulting in pyogenic liver abscesses. these infections are caused by hypervirulent k. pneumoniae (hvkp) isolates primarily of capsule serotype k1 or k2. hypervirulent k1 isolates belong to clonal complex 23 (cc23), indicating that this clonal lineage has a specific genetic background conferring hypervirulence. here, we apply whole-genome sequencing to a collection of k. pneumoniae isolates to characteriz ... | 2015 | 26199326 |
| genomic analysis of the emergence and rapid global dissemination of the clonal group 258 klebsiella pneumoniae pandemic. | multidrug-resistant klebsiella pneumoniae producing the kpc carbapenemase have rapidly spread throughout the world, causing severe healthcare-associated infections with limited antimicrobial treatment options. dissemination of kpc-producing k. pneumoniae is largely attributed to expansion of a single dominant strain, st258. in this study, we explore phylogenetic relationships and evolution within st258 and its clonal group, cg258, using whole genome sequence analysis of 167 isolates from 20 coun ... | 2015 | 26196384 |
| antibiotic resistance pattern and clinical outcomes in acute cholecystitis: 567 consecutive worldwide patients in a prospective cohort study. | postoperative complications for cholecystitis and cholelithiasis are important causes of intra-abdominal infections (iais). there have been no previous reports on intra-abdominal bacteriology in complicated iais due to acute cholecystitis (ac). | 2015 | 26192973 |
| impact of carbapenem resistance on epidemiology and outcomes of nonbacteremic klebsiella pneumoniae infections. | although high mortality associated with carbapenem-resistant klebsiella pneumoniae (crkp) bacteremia has been well described, the epidemiology and outcomes of nonbacteremic infection are unknown. | 2015 | 26190386 |
| structure and function of cutc choline lyase from human microbiota bacterium klebsiella pneumoniae. | cutc choline trimethylamine-lyase is an anaerobic bacterial glycyl radical enzyme (gre) that cleaves choline to produce trimethylamine (tma) and acetaldehyde. in humans, tma is produced exclusively by the intestinal microbiota, and its metabolite, trimethylamine oxide, has been associated with a higher risk of cardiovascular diseases. therefore, information about the three-dimensional structures of tma-producing enzymes is important for microbiota-targeted drug discovery. we have cloned, express ... | 2015 | 26187464 |