| a case of liver abscess caused by tigecycline-nonsusceptible klebsiella pneumoniae. | | 2016 | 27247260 |
| pathways regulating the pbgp operon and colistin resistance in klebsiella pneumoniae strains. | in this study, we investigated colistin resistance mechanisms associated with the regulation of the pbgp operon in klebsiella pneumoniae, using four isogenic pairs of colistin-susceptible strains and their colistin-resistant derivatives and two colistin-resistant clinical isolates. amino acid sequence alterations of phopq, pmrab, and mgrb were investigated, and mrna expression levels of phoq, pmrb, pmrd, and pbgp were measured using quantitative real-time pcr. the phoq and pmrb genes were delete ... | 2016 | 27238937 |
| galleria mellonella as a model system to assess the efficacy of antimicrobial agents against klebsiella pneumoniae infection. | | 2016 | 27237961 |
| prevalence of extended-spectrum beta-lactamase-producing klebsiella pneumoniae isolates in nosocomial and community-acquired urinary tract infections. | klebsiella pneumoniae is a family member of enterobacteriaceae. isolates of k. pneumoniae produce enzymes that cause decomposition of third generation cephalosporins. these enzymes are known as extended-spectrum beta-lactamase (esbl). resistance of k. pneumoniae to beta-lactamase antibiotics is commonly mediated by beta-lactamase genes. | 2016 | 27226874 |
| [investigation of antimicrobial resistance of klebsiella pneumoniae and pseudomonas aeruginosa isolates from rat-like animals around a hospital in guangzhou]. | to investigate antimicrobial resistance of klebsiella pneumoniae and pseudomonas aeruginosa isolates in fecal samples from rat-like animals. | 2016 | 27222186 |
| the postantibiotic effect and post-β-lactamase-inhibitor effect of ceftazidime, ceftaroline and aztreonam in combination with avibactam against target gram-negative bacteria. | the magnitudes of the postantibiotic effect (pae) and post-β-lactamase-inhibitory effect (plie) of ceftazidime-avibactam, ceftaroline-avibactam, and aztreonam-avibactam were determined against isolates of enterobacteriaceae and pseudomonas aeruginosa that either harboured genes encoding serine and/or metallo-β-lactamases, or did not harbour bla genes. the bla genes included ones that encoded extended spectrum β-lactamases, ampc and kpc β-lactamases, and one metallo-β-lactamase, ndm-1. no substan ... | 2016 | 27221329 |
| tigecycline susceptibility and molecular resistance mechanisms among clinical klebsiella pneumoniae strains isolated during non-tigecycline treatment. | tigecycline is one of the few therapeutic options that are available for treating serious clinical infections. however, tigecycline nonsusceptible enterobacteriaceae has emerged recently in china. in this study, a total of 28 clinical klebsiella pneumoniae isolates that were not previously exposed to tigecycline were collected and confirmed for tigecycline minimum inhibitory concentrations (mics) using standard broth microdilution tests. to elucidate the mechanisms underlying molecular resistanc ... | 2017 | 27219271 |
| in vivo pharmacodynamic target assessment of delafloxacin against staphylococcus aureus, streptococcus pneumoniae, and klebsiella pneumoniae in a murine lung infection model. | delafloxacin is a broad-spectrum anionic fluoroquinolone under development for the treatment of bacterial pneumonia. the goal of the study was to determine the pharmacokinetic/pharmacodynamic (pk/pd) targets in the murine lung infection model for staphylococcus aureus, streptococcus pneumoniae, and klebsiella pneumoniae four isolates of each species were utilized for in vivo studies: for s. aureus, one methicillin-susceptible and three methicillin-resistant isolates; s. pneumoniae, two penicilli ... | 2016 | 27216072 |
| modeling meropenem treatment, alone and in combination with daptomycin, for kpc-producing klebsiella pneumoniae strains with unusually low carbapenem mics. | klebsiella pneumoniae strains producing k. pneumoniae carbapenemase (kpc) cause serious infections in debilitated and immunocompromised patients and are associated with prolonged hospital stays and increased mortality rates. daptomycin is a lipopeptide used against staphylococcus aureus infection and considered inactive against gram-negative bacteria. we investigated the effectiveness of a daptomycin-meropenem combination by synergy kill curve and a pharmacokinetic/pharmacodynamic model. the com ... | 2016 | 27216067 |
| multidrug-resistant bacteria in the community: trends and lessons learned. | multidrug resistant (mdr) bacteria are one of the most important threats to public health. typically, mdr bacteria are associated with nosocomial infections. however, some mdr bacteria have become prevalent causes of community-acquired infections. the spread of mdr bacteria into the community is a crucial development, and is associated with increased morbidity, mortality, health care costs, and antibiotic use. factors associated with community dissemination of mdr bacteria overlap but are distin ... | 2016 | 27208764 |
| [septic shock in pyogenic liver abscess: clinical considerations]. | pyogenic liver abscess (pla) is a life-threatening condition, despite advances in diagnostic technology and strategies for treatment. a strong predictor of mortality in this condition is septic shock. this study describes clinical, biochemical, and radiologic features in patients with pla with or without septic shock, with the intent of describing risk factors for septic shock. | 2016 | 27206435 |
| predictors of mortality in korean patients with pyogenic liver abscess: a single center, retrospective study. | the mortality rate of pyogenic liver abscess (pla) has decreased dramatically, but it remains a potentially life threatening disease. most cases are cryptogenic or occur in elderly men with underlying biliary tract disease. although several studies have addressed the characteristics and etiology of pla, research on factors affecting pla-associated mortality is lacking. this study intended to identify the clinical and radiological features, pathogens, complications, and predictors of mortality in ... | 2016 | 27206434 |
| molecular epidemiology of carbapenem-resistant klebsiella pneumoniae in greece. | hospital infections caused by carbapenem-resistant klebsiella pneumoniae (crkp) constitute a worldwide problem associated with high rates of treatment failure and mortality. in greece, crkp have emerged in 2002 due to vim carbapenemase production and later due to kpc, ndm and oxa-48-like carbapenemases that have become endemic. the molecular epidemiology of crkp strains is dynamic, as antibiotic consumption and worldwide traveling are strongly associated with global spread of crkp isolates. late ... | 2016 | 27206024 |
| draft genome sequence of a multidrug-resistant klebsiella pneumoniae strain isolated from king abdullah medical city, makkah, saudi arabia. | multidrug-resistant (mdr) gram-negative infections represent a growing problem and a serious global threat. carbapenem-resistant klebsiella pneumoniae is perhaps cause the most difficult infection to treat and is associated with increased morbidity and mortality. here, we report the draft genome sequence of an mdr k. pneumoniae strain isolated from makkah, saudi arabia. | 2016 | 27198017 |
| scalable and environmentally benign process for smart textile nanofinishing. | a major challenge in nanotechnology is that of determining how to introduce green and sustainable principles when assembling individual nanoscale elements to create working devices. for instance, textile nanofinishing is restricted by the many constraints of traditional pad-dry-cure processes, such as the use of costly chemical precursors to produce nanoparticles (nps), the high liquid and energy consumption, the production of harmful liquid wastes, and multistep batch operations. by integrating ... | 2016 | 27196424 |
| bloodstream-to-eye infections are facilitated by outer blood-retinal barrier dysfunction. | the blood-retinal barrier (brb) functions to maintain the immune privilege of the eye, which is necessary for normal vision. the outer brb is formed by tightly-associated retinal pigment epithelial (rpe) cells which limit transport within the retinal environment, maintaining retinal function and viability. retinal microvascular complications and rpe dysfunction resulting from diabetes and diabetic retinopathy cause permeability changes in the brb that compromise barrier function. diabetes is the ... | 2016 | 27195776 |
| secular trends in klebsiella pneumoniae isolated in a tertiary-care hospital: increasing prevalence and accelerated decline in antimicrobial susceptibility. | introduction klebsiella pneumoniae has become an increasingly important etiologic agent of nosocomial infections in recent years. this is mainly due to the expression of virulence factors and development of resistance to several antimicrobial drugs. methods this retrospective study examines data obtained from the microbiology laboratory of a brazilian tertiary-care hospital. to assess temporal trends in prevalence and antimicrobial susceptibility, k. pneumoniae isolates were analyzed from 2000 t ... | 2016 | 27192586 |
| antibiotic adjuvant therapy for multi-drug resistant carbapenemases producing klebsiella pneumoniae associated sepsis: a case study. | rising resistance and spread of k. pneumoniae strains, create great concerns in treating sepsis patients due to high incidence of mortality and morbidity. the current study is a case of a 20-year-old male with sepsis and bilateral lung lesions infected with multi-drug resistant (mdr) carbapenemase producing k. pneumoniae (kpc) showing resistance to carbapenem and polymyxin. based on sensitivity report, patient was put on antibiotic adjuvant: elores (ceftriaxone, sulbactam, disodium edetate) alon ... | 2016 | 27190808 |
| ndm-4- and ndm-5-producing klebsiella pneumoniae coinfection in a 6-month-old infant. | | 2016 | 27185797 |
| increased biofilm formation ability in klebsiella pneumoniae after short-term exposure to a simulated microgravity environment. | biofilm formation is closely related to the pathogenetic processes of klebsiella pneumoniae, which frequently causes infections in immunocompromised individuals. the immune system of astronauts is compromised in spaceflight. accordingly, k. pneumoniae, which used to be isolated from orbiting spacecraft and astronauts, poses potential threats to the health of astronauts and mission security. microgravity is a key environmental cue during spaceflight. therefore, determining its effects on bacteria ... | 2016 | 27185296 |
| a case of lobar pneumonia and sepsis with death caused by invasive klebsiella rhinoscleromatis infection. | klebsiella pneumoniae often causes pneumonia and other infections in heavy drinkers and patients with diabetes. pneumonia caused by klebsiella rhinoscleromatis, a subspecies of k. pneumoniae, has not been previously reported. we report a case of pneumonia caused by k. rhinoscleromatis. a 68-year-old man with type 2 diabetes visited our department complaining fever and fatigue for 10 days and cough and bloody sputum for two days. his japan coma scale score was i-1, body temperature 38.3 °c, blood ... | 2016 | 27184935 |
| characterization of the genetic environment of the blakpc-2 gene among klebsiella pneumoniae isolates from a chinese hospital. | infection caused by carbapenem-resistant klebsiella pneumoniae has become a major healthcare threat and kpc-2 enzyme is a dominant factor mediating carbapenems resistance in k. pneumoniae. this study was designed to determine the genetic environment of blakpc-2, which prevailed in clinical k. pneumoniae isolates recovered in huashan hospital, shanghai, china. forty-two clinical isolates were included in this study by blakpc-2 screening. after multilocus sequence typing and plasmid analyses of pc ... | 2017 | 27183358 |
| structural determination of the polysaccharide isolated from biofilms produced by a clinical strain of klebsiella pneumoniae. | klebsiella pneumoniae are gram negative opportunistic pathogens producing capsular (k) polysaccharides. seventy-seven different k antigens have been described and they are the basis for k serotyping. capsular polysaccharides are important virulence factors and have a relevant role for the structure of biofilm communities. nevertheless, little information is available on the polysaccharides produced in biofilm matrices by klebsiella spp. in the present study, a clinical isolate of klebsiella pneu ... | 2016 | 27182661 |
| antiradical and antimicrobial activity of phenolic fractions obtained from honeys. | honey is a natural product consisting of multiple components which determine its dietary and medicinal properties. in this work there were studied methanol fractions obtained from seven honeys from lower silesia (poland) collected in different seasons of three successive years. melissopalynologic studies revealed that two of them were polyfloral, and five were classified as monofloral (two buckwheat and three rapes). the amount of phenolic compounds in honeys varied from 0.09 to 0.38 mg per g of ... | 2016 | 27180430 |
| in vitro and in vivo efficacy, toxicity, bio-distribution and resistance selection of a novel antibacterial drug candidate. | a synthetic antimicrobial peptide was identified as a possible candidate for the development of a new antibacterial drug. the peptide, set-m33l, showed a mic90 below 1.5 μm and 3 μm for pseudomonas aeruginosa and klebsiella pneumoniae, respectively. in in vivo models of p. aeruginosa infections, the peptide and its pegylated form (set-m33l-peg) enabled a survival percentage of 60-80% in sepsis and lung infections when injected twice i.v. at 5 mg/kg, and completely healed skin infections when adm ... | 2016 | 27169671 |
| persisting transmission of carbapenemase-producing klebsiella pneumoniae due to an environmental reservoir in a university hospital, france, 2012 to 2014. | in france, the proportion of episodes of carbapenemase-producing enterobacteriaceae (cpe) with no recent stay or hospitalisation abroad is increasing. in this study, we investigate epidemiological links between apparently unrelated cases of oxa-48-producing klebsiella pneumoniae (kp oxa-48) colonisation or infection. we genotyped detected organisms by repetitive sequence-based pcr, and used a dynamic registry of cases and contacts to cross-reference patients' hospital stays. between 1 november 2 ... | 2016 | 27168586 |
| appropriateness of empirical therapy in carbapenem-resistant klebsiella pneumoniae intensive care unit acquired infection: role of surveillance cultures. | | 2016 | 27167235 |
| correction: elucidation of the rama regulon in klebsiella pneumoniae reveals a role in lps regulation. | [this corrects the article doi: 10.1371/journal.ppat.1004627.]. | 2016 | 27166748 |
| the volatile metabolome of klebsiella pneumoniae in human blood. | klebsiella pneumoniae is an important cause of bloodstream infections in critically-ill patients, with mortality exceeding 50% for infections caused by antibiotic-resistant strains. despite its importance as a bacteremia-causing agent, there is little known about the metabolism of k. pneumoniae during growth in pure human blood. here, we comprehensively profile the volatile metabolites produced by k. pneumoniae during growth in human blood to approximately mid-exponential (7 h) and early station ... | 2016 | 27163334 |
| structural and functional characterization of the lps transporter lptde from gram-negative pathogens. | incorporation of lipopolysaccharide (lps) into the outer membrane of gram-negative bacteria is essential for viability, and is accomplished by a two-protein complex called lptde. we solved crystal structures of the core lptde complexes from yersinia pestis, klebsiella pneumoniae, pseudomonas aeruginosa, and a full-length structure of the k. pneumoniae lptde complex. our structures adopt the same plug and 26-strand β-barrel architecture found recently for the shigella flexneri and salmonella typh ... | 2016 | 27161977 |
| effects of mutation of 2,3-butanediol formation pathway on glycerol metabolism and 1,3-propanediol production by klebsiella pneumoniae j2b. | the current study investigates the impact of mutation of 2,3-butanediol (bdo) formation pathway on glycerol metabolism and 1,3-propanediol (pdo) production by lactate dehydrogenase deficient mutant of klebsiella pneumoniae j2b. to this end, bdo pathway genes, buda, budb, budc and budo (whole-bud operon), were deleted from k. pneumoniae j2b δldha and the mutants were studied for glycerol metabolism and alcohols (pdo, bdo) production. δbudo-mutant-only could completely abolish bdo production, but ... | 2016 | 27160953 |
| emergence of serratia marcescens isolates possessing carbapenem-hydrolysing β-lactamase kpc-2 from china. | eighty-three carbapenem-resistant serratia marcescens isolates were recovered from zhejiang provincial people's hospital, china. the minimum inhibitory concentrations of imipenem, meropenem, and ertapenem for all isolates were 2 to >128 μg/ml. polymerase chain reaction indicated that 63 s. marcescens isolates produced klebsiella pneumoniae carbapenemase (kpc)-2. clone a (15 isolates) and clone b (41 isolates) were the two dominant clones and clone a strains were gradually replaced by clone b str ... | 2016 | 27160868 |
| impact of glycemic control on capsular polysaccharide biosynthesis and opsonophagocytosis of klebsiella pneumoniae: implications for invasive syndrome in patients with diabetes mellitus. | klebsiella pneumoniae (kp), with production of abundant capsular polysaccharide (cps), is capable of causing invasive syndrome. environmental glucose stimuli may increase cps biosynthesis. we aimed to investigate the relationship between glycemic control and kp-mediated invasive syndrome in diabetic patients and the effect of glucose on cps biosynthesis. diabetic patients with community-acquired kp bacteremia were included to study the risk factors of invasive syndrome. kp-m1, a serotype-k1 kp c ... | 2016 | 27159655 |
| [endogenous endophthalmitis complicating klebsiella pneumoniae liver abscess]. | | 2016 | 27158099 |
| draft genome sequence of klebsiella pneumoniae ucd-ja29 isolated from a patient with sepsis. | here, we present the 6,155,188-bp draft genome sequence of klebsiella pneumoniae ucd-ja29, isolated from blood cultures from a patient with sepsis at the university of california, davis medical center in sacramento, california, usa. | 2016 | 27151785 |
| first report of a verona integron-encoded metallo-β-lactamase-producing klebsiella pneumoniae infection in a child in the united states. | we report the first case of a child in the united states infected with an organism producing a verona integron-encoded metallo-β-lactamase. this child succumbed to a ventilator-associated pneumonia caused by a klebsiella pneumoniae producing this resistance mechanism. | 2016 | 27147714 |
| comparative analyses of phenotypic methods and 16s rrna, khe, rpob genes sequencing for identification of clinical isolates of klebsiella pneumoniae. | the present work aimed to evaluate 16s rrna, khe and rpob gene sequencing for the identification of klebsiella pneumoniae in comparison with phenotypic methods. fifteen clinical isolates were examined, which were initially identified as k. pneumoniae subsp. pneumoniae using the automated vitek 32 system in two hospitals in enshi city, china. their identity was further supported by conventional phenotypic methods on the basis of morphological and biochemical characteristics. using bayesian phylog ... | 2016 | 27147066 |
| antimicrobial hyaluronic acid-cefoxitin sodium thin films produced by electrospraying. | the healing properties of hyaluronic acid (ha) in the recovery of wounds are well known. cefoxitin (cef), a cephalosporin antibiotic, is generally used to prevent and treat postoperative infections. in this study, we describe the incorporation of cef in ha thin films (cef-haf) by using electrospraying. scanning electron microscopy images showed that ha-containing thin films (haf) were composed of numerous nanoparticles (255 ± 177 nm in diameter) with irregular surfaces, connected to each other w ... | 2016 | 27146506 |
| heteroresistance to colistin in klebsiella pneumoniae is triggered by small colony variants sub-populations within biofilms. | the emergence of klebsiella pneumoniae multidrug-resistant strains paves the way to the re-introduction of colistin as a salvage therapy. however, recent planktonic studies have reported several cases of heteroresistance to this antimicrobial agent. the aim of this present work was to gain better understanding about the response of k. pneumoniae biofilms to colistin antibiotherapy and inspect the occurrence of heteroresistance in biofilm-derived cells. biofilm formation and its susceptibility to ... | 2016 | 27140200 |
| pharmacodynamic evaluation of the potential clinical utility of fosfomycin and meropenem in combination therapy against kpc-2-producing klebsiella pneumoniae. | kpc-producing klebsiella pneumoniae causes serious infections associated with high death rates worldwide. combination therapy consisting of fosfomycin and a carbapenem is better than monotherapy to combat multidrug-resistant microorganisms, but no dosages for the combination have been defined. the mics of meropenem and fosfomycin were evaluated against 18 clinical isolates of kpc-2-producing k. pneumoniae the activities of combination antimicrobials were also determined by the checkerboard metho ... | 2016 | 27139468 |
| hypermucoviscous klebsiella pneumoniae pneumonia: follow the string! | | 2016 | 27139020 |
| colistin-sparing regimens against klebsiella pneumoniae carbapenemase-producing k. pneumoniae isolates: combination of tigecycline or doxycycline and gentamicin or amikacin. | in vitro studies of the combination of an aminoglycoside with tigecycline or doxycycline against klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae isolates are rarely published. the goal of this study was to evaluate the antibacterial activity of the combination regimens. | 2016 | 27133391 |
| first clinical cases of ndm-1-producing klebsiella pneumoniae from two hospitals in bulgaria. | we report the first confirmed cases of ndm-1-producing klebsiella pneumoniae infections in two hospitals in bulgaria. the isolates were diverse in terms of plasmid and co-resistance gene content. k. pneumoniae pr2682, causing sepsis in patient with polytrauma due to traffic accident, harbored blandm-1,blacmy-4, blactx-m-15, blashv-1, blatem-1b, qnrb, and aac(6')-ib. blandm-1 was transferable by conjugation and located on an inca/c plasmid of 176-kb, which also carried blacmy-4, blactx-m-15, blat ... | 2016 | 27129373 |
| a comparative study of lung host defense in murine obesity models. insights into neutrophil function. | we have shown that obesity-associated attenuation of murine acute lung injury is driven, in part, by blunted neutrophil chemotaxis, yet differences were noted between the two models of obesity studied. we hypothesized that obesity-associated impairment of multiple neutrophil functions contributes to increased risk for respiratory infection but that such impairments may vary between murine models of obesity. we examined the most commonly used murine obesity models (diet-induced obesity, db/db, cp ... | 2016 | 27128821 |
| characterization of an outbreak due to ctx-m-15-producing klebsiella pneumoniae lacking the blaoxa-48 gene belonging to clone st405 in a neonatal unit in southern spain. | | 2016 | 27118773 |
| prevalence and susceptibility patterns of bacteria causing respiratory tract infections in north waziristan, pakistan. | respiratory tract infections (rtis) are the most common infectious diseases in humans and are the major cause of mortality and morbidity in pakistan. these infections are the leading causes of consultations in primary care in pakistan. therefore, this study was aimed at determining bacterial pathogens of respiratory tract infections and the susceptibility patterns of bacterial isolates to antibiotics. the study was conducted between february, 2013 and march, 2014 in north waziristan region of pa ... | 2016 | 27113300 |
| carbapenemase-bearing klebsiella spp. in sink drains: investigation into the potential advantage of copper pipes. | sink drains have long been known to harbour pathogenic bacteria and efforts such as heated sink traps have been made to control them. sink outlet pipes have been implicated in outbreaks of infection by multi-resistant klebsiella pneumoniae. to investigate whether a change to copper pipes might prevent cross-infection, sections of standard sink outlet pipe were left in containers of water to which multi-resistant human strains of k. pneumoniae had been added. bacterial counts from the water of co ... | 2016 | 27112043 |
| klebsiella pneumoniae sepsis deteriorated by uncontrolled underlying disease in a decontamination worker in fukushima, japan. | patients with underlying conditions are at a higher risk of developing sepsis, a systematic response to infection, which has a high mortality rate. after the march 2011 fukushima daiichi nuclear power plant accident, there has been an influx of migrant decontamination workers; however, little is known about their health status. | 2016 | 27108638 |
| complete nucleotide sequence of ph11, an inchi2 plasmid conferring multi-antibiotic resistance and multi-heavy metal resistance genes in a clinical klebsiella pneumoniae isolate. | the complete 284,628bp sequence of ph11, an inchi2 plasmid, was determined through single-molecule, real-time (smrt) sequencing. harbored by a clinical klebsiella pneumoniae strain h11, and isolated in beijing, this plasmid contains multiple antibiotic resistance genes, including cata2, aac(6')-ib, strb, stra, dfra19, blatem-1, blashv-12, sul1, qace delta 1, erea, arr2, and aac3. the aac(6')-ib is carried by a class i integron. plasmid ph11 also carries several genes associated with resistance t ... | 2016 | 27101788 |
| carbapenem-resistant enterobacteriaceae infections in children: an italian retrospective multicenter study. | the spread of carbapenem-resistant enterobacteriaceae (cre) is a health problem of major concern. cre-related infections have significant morbidity and mortality, but data on cre infection in pediatric population are limited. the aim of this study was to analyze epidemiologic and clinical characteristics, risk factors, therapeutic options and outcome of cre infections in children in italy. | 2016 | 27100130 |
| haemoptysis in a female with diabetes mellitus: a unique presentation of chronic pulmonary aspergillosis, pulmonary tuberculosis, and klebsiella peumoniae co-infection. | while chronic pulmonary aspergillosis (cpa), pulmonary tuberculosis (ptb), and klebsiella pneumoniae pneumonia co-infection is rare, we present a 50-year-old woman with uncontrolled diabetes who presented with these three diseases. there is considerable overlap in symptoms of ptb and cpa. treatment with antifungals, anti-tuberculosis therapy, and antibiotics is beneficial. | 2016 | 27099746 |
| evaluation of biofilm formation among klebsiella pneumoniae isolates and molecular characterization by eric-pcr. | klebsiella pneumoniae is among the most frequently recovered etiologic agents from nosocomial infections. this opportunistic pathogen can generate a thick layer of biofilm as one of its important virulence factors, enabling the bacteria to attach to living or abiotic surfaces, which contributes to drug resistance. | 2016 | 27099694 |
| ceftazidime/avibactam and ceftolozane/tazobactam: second-generation β-lactam/β-lactamase inhibitor combinations. | ceftolozane/tazobactam and ceftazidime/avibactam are 2 novel β-lactam/β-lactamase combination antibiotics. the antimicrobial spectrum of activity of these antibiotics includes multidrug-resistant (mdr) gram-negative bacteria (gnb), including pseudomonas aeruginosa. ceftazidime/avibactam is also active against carbapenem-resistant enterobacteriaceae that produce klebsiella pneumoniae carbapenemases. however, avibactam does not inactivate metallo-β-lactamases such as new delhi metallo-β-lactamases ... | 2016 | 27098166 |
| influence of ethanol concentration in the phagocytic function of neutrophils against klebsiella pneumoniae isolates in an experimental model. | although the prevalence of pneumonia or other extrapulmonary infections is higher in people with alcoholism or acute alcohol intoxication, the possible relationship of acute alcohol intoxication to phagocytic function has not been investigated. our aim was to determine whether acute alcohol intoxication suppresses phagocytic function in human neutrophils. | 2016 | 27094384 |
| knowledge, attitudes, and beliefs regarding antimicrobial therapy and resistance among physicians in alexandria university teaching hospitals and the associated prescription habits. | irresponsible prescription of antimicrobials (ams) is the driving factor for the growing antimicrobial resistance (amr) crisis. in this study, we assessed the knowledge, attitudes, perceptions, and beliefs regarding ams and amr together with the prescription habits of physicians in three university hospitals in alexandria, egypt. a 40-question survey was used. physicians were stratified into residents and practicing staff members, and further into various departments. clinical pharmacists at the ... | 2017 | 27092847 |
| antibiotic susceptibility pattern and identification of extended spectrum β-lactamases (esbls) in clinical isolates of klebsiella pneumoniae from shiraz, iran. | klebsiella pneumoniae, one of the important causes of nosocomial infections, is the most common extended spectrum β-lactamases (esbls) producing organism. esbls are defined as the enzymes capable of hydrolyzing oxyimino-cephalosporins, monobactams and carbapenems. the aims of this study were to identify esbl-producing k. pneumoniae isolates and detect their antibiotic susceptibility pattern. | 2016 | 27092225 |
| tannic acid degradation by klebsiella strains isolated from goat feces. | tannins are toxic polyphenols that either bind and precipitate or condense proteins. the high tannin content of some plants is the preliminary limitation of using them as a ruminant feed. so, the aim of this study was the isolation and characterization of tannic acid degrading bacterial strains from goat feces before and after feeding on pistachio-soft hulls as tannin rich diet (trd). | 2016 | 27092220 |
| potential for cefazolin as de-escalation therapy for klebsiella pneumoniae bacteraemia. | | 2015 | 27090077 |
| first case of bacteremic liver abscess caused by an st260-related (st1861), hypervirulent klebsiella pneumoniae. | | 2016 | 27084307 |
| continuous evaluation of changes in the serum proteome from early to late stages of sepsis caused by klebsiella pneumoniae. | serum protein profiles of patients with bacterial sepsis from the day of diagnosis until recovery/mortality were compared from early to late stages in response to severe sepsis using two dimensional electrophoresis. the proteins exhibiting changes during the course of sepsis (20‑28 day mortality) were selected and identified by matrix‑assisted laser desorption ionization‑time of flight‑tandem mass spectrometry. among the proteins identified, haptoglobin (hp), transthyretin (ttr), orosomucoid 1/α ... | 2016 | 27082932 |
| complete genome sequence of klebsiella pneumoniae yh43. | we report here the complete genome sequence ofklebsiella pneumoniaestrain yh43, isolated from sweet potato. the genome consists of a single circular chromosome of 5,520,319 bp in length. it carries 8 copies of rrna operons, 86 trna genes, 5,154 protein-coding genes, and thenifgene cluster for nitrogen fixation. | 2016 | 27081127 |
| a prospective study of wound infection among post-discharge patients at a level 1 trauma centre of india. | to study the rate of wound infections in the post-discharged patient population and to assess the usefulness of post-discharge surveillance. | 2016 | 27080772 |
| menacing emergence of fosfomycin resistance among klebsiella pneumoniae carbapenemase-2-producing k. pneumoniae driven by prior use in critically ill patients. | | 2016 | 27079463 |
| extended antibiotic resistance in carbapenemase-producing klebsiella pneumoniae: a case series. | infections caused by carbapenemase-producing klebsiella pneumoniae resistant to tigecycline, colistin, or aminoglycosides are a growing health concern. in our retrospective chart review, we noted increased resistance to colistin compared with tigecycline, despite limited prior use of colistin. this may affect the choice of presumptive antibiotics used in these hard to treat infections. improved infection control and antimicrobial stewardship practices are essential to prevent the spread of these ... | 2016 | 27079242 |
| carbapenemase-producing klebsiella pneumoniae: a major clinical challenge. | | 2016 | 27078606 |
| frequency, antimicrobial resistance and genetic diversity of klebsiella pneumoniae in food samples. | this study aimed to assess the frequency of klebsiella pneumoniae in food samples and to detect antibiotic resistance phenotypes, antimicrobial resistance genes and the molecular subtypes of the recovered isolates. a total of 998 food samples were collected, and 99 (9.9%) k. pneumoniae strains were isolated; the frequencies were 8.2% (4/49) in fresh raw seafood, 13.8% (26/188) in fresh raw chicken, 11.4% (34/297) in frozen raw food and 7.5% (35/464) in cooked food samples. antimicrobial resistan ... | 2016 | 27078494 |
| clinical and molecular analysis of klebsiella pneumoniae causing liver abscess in china. | hypervirulent klebsiella pneumoniae (hvkp) has been characterized as the major pathogen associated with the development of liver abscesses. although the prevalence of k. pneumoniae in liver abscesses in chinese patients is on the rise, methods for the early laboratory identification and recognition of hvkp are lacking, and hvkp detection and surveillance are thus limited. | 2016 | 27073997 |
| understanding, preventing and eradicating klebsiella pneumoniae biofilms. | the ability of pathogenic bacteria to aggregate and form biofilm represents a great problem for public health, since they present extracellular components that encase these micro-organisms, making them more resistant to antibiotics and host immune attack. this may become worse when antibiotic-resistant bacterial strains form biofilms. however, antibiofilm screens with different compounds may reveal potential therapies to prevent/treat biofilm infections. here, we focused on klebsiella pneumoniae ... | 2016 | 27064296 |
| complete genome sequence of kpc-3- and ctx-m-15-producing klebsiella pneumoniae sequence type 307. | klebsiella pneumoniaesequence type (st) 307, carryingblakpc-3,blactx-m-15,blaoxa-1,aac(6')-ib-cr, andqnrb1 genes, is replacing the predominant hyperepidemic st258 clone in italy. whole-genome and complete plasmid sequencing of one st307 strain was performed and new features were identified. | 2016 | 27056222 |
| successful treatment of a carbapenem-resistant klebsiella pneumoniae carrying bla oxa-48 , bla vim-2 , bla cmy-2 and bla shv- with high dose combination of imipenem and amikacin. | we describe a case of 58-year-old man with septic shock due to carbapenem-resistant klebsiella pneumoniae (cr-kp) bloodstream infections (bsi) who was successfully treated with a high dose association of amikacin and imipenem combined with continuous venovenous hemodiafiltration (cvvhdf). a klebsiella pneumoniae (kp) was isolated from the catheter culture and from two blood samples, drawn from the catheter before removal and from a peripheral vein. the kp was intermediate to amikacin (mic = 16 μ ... | 2016 | 27051575 |
| structure of a quinolone-stabilized cleavage complex of topoisomerase iv from klebsiella pneumoniae and comparison with a related streptococcus pneumoniae complex. | klebsiella pneumoniae is a gram-negative bacterium that is responsible for a range of common infections, including pulmonary pneumonia, bloodstream infections and meningitis. certain strains of klebsiella have become highly resistant to antibiotics. despite the vast amount of research carried out on this class of bacteria, the molecular structure of its topoisomerase iv, a type ii topoisomerase essential for catalysing chromosomal segregation, had remained unknown. in this paper, the structure o ... | 2016 | 27050128 |
| emergence of kpc-producing klebsiella pneumoniae hypervirulent clone of capsular serotype k1 that belongs to sequence type 11 in mainland china. | kpc-2 has been rarely reported in hypervirulent klebsiella pneumoniae strains. here, we describe a kpc-2-producing k. pneumoniae hypervirulent clone of capsular serotype k1 belonging to sequence type 11. the presence of kpc carbapenemase in hypervirulent clone could mark an evolutionary step toward its establishment as major nosocomial pathogen. | 2016 | 27049969 |
| population pharmacokinetics and probability of target attainment of meropenem in critically ill patients. | patients admitted to intensive care unit (icu) with klebsiella pneumoniae infections are characterized by high mortality. the aims of the present study were to investigate the population pharmacokinetics parameters and to assess the probability of target attainment of meropenem in critically ill patients to provide information for more effective regimens. | 2016 | 27048201 |
| validation of a high-throughput screening assay for identification of adjunctive and directly acting antimicrobials targeting carbapenem-resistant enterobacteriaceae. | we describe development and validation of a high-throughput screen (hts) for identifying small molecules that restore the efficacy of carbapenems (adjunctives) and/or directly inhibit growth of carbapenem-resistant enterobacteriaceae (cre). our hts assay is based on a screen-counterscreen approach using a representative multidrug-resistant cre strain, klebsiella pneumoniae bidmc12a. specifically, we tested the ability of small molecules to inhibit bacterial growth in the presence (screen) or abs ... | 2016 | 27045615 |
| combination regimens for treatment of carbapenem-resistant klebsiella pneumoniae bloodstream infections. | previous studies reported decreased mortality in patients with carbapenemase-producing klebsiella pneumoniae bloodstream infections (bsis) treated with combination therapy but included carbapenem-susceptible and -intermediate isolates, as per revised clsi breakpoints. here, we assessed outcomes in patients with bsis caused by phenotypically carbapenem-resistant k. pneumoniae (crkp) according to the number of in vitro active agents received and whether an extended-spectrum beta-lactam (bl) antibi ... | 2016 | 27044555 |
| a neonatal septic arthritis case caused by klebsiella pneumoniae: a case report. | septic arthritis is encountered very rarely during the neonatal period and its diagnosis can delay because of atypical symptoms, thus it may lead to serious sequelae. the sequale can be prevented by early diagnosis and concomitant treatment. in neonates, pain can be experienced as a result of pseudoparalysis and of movement of the effected joints. a 17-day-old neonatal patient was brought to our hospital with complaint of unrest and then diagnosed with septic arthritis due to propagation of kleb ... | 2016 | 27042550 |
| innate lymphocyte/ly6c(hi) monocyte crosstalk promotes klebsiella pneumoniae clearance. | increasing antibiotic resistance among bacterial pathogens has rendered some infections untreatable with available antibiotics. klebsiella pneumoniae, a bacterial pathogen that has acquired high-level antibiotic resistance, is a common cause of pulmonary infections. optimal clearance of k. pneumoniae from the host lung requires tnf and il-17a. herein, we demonstrate that inflammatory monocytes are rapidly recruited to the lungs of k. pneumoniae-infected mice and produce tnf, which markedly incre ... | 2016 | 27040495 |
| biofilms associated with bowel necrosis: a newly recognised phenomenon in infants. | a biofilm is defined as a collection of organisms attached to a surface and surrounded by a matrix. | 2016 | 27032844 |
| emergence of klebsiella pneumoniae st273 carrying blandm-7 and st656 carrying blandm-1 in manila, philippines. | we sought to determine the epidemiology of carbapenem-resistant enterobacteriaceae and to investigate the emergence of carbapenem-resistant klebsiella pneumoniae in two teaching hospitals in manila, philippines. we screened 364 enterobacteriaceae for carbapenem resistance between 2012 and 2013 and detected four carbapenem-resistant k. pneumoniae isolates from three different patients. we used whole genome sequencing to determine the antibiotic resistance profiles and confirmed the presence of ca ... | 2016 | 27032000 |
| the investigation of oxacillinase/metallo-beta-lactamase genes and clonal analysis in carbapenem-resistant klebsiella pneumoniae. | infections due to carbapenem-resistant klebsiella pneumoniae represent a growing problem nationally. in our study, we aimed to examine carbapenem-resistant k. pneumoniae with multiple resistance isolated in the intensive care unit of our hospital. isolates were investigated for the presence of oxacillinase and metallo-beta lactamase genes with a view to determining the clonal relationship between the strains intensely over a short period. strain identification was completed with conventional met ... | 2016 | 27031897 |
| the combined-disk boronic acid test as an accurate strategy for the detection of kpc carbapenemase in central america. | carbapenemase-producing klebsiella pneumoniae (kpc) outbreaks may cause a huge economical burden on developing countries. furthermore, kpc can be challenging to detect. we describe the laboratory strategy for the detection of kpc from 2011 to 2013 in a tertiary care hospital in central america with approximately 1,000 beds. | 2016 | 27031462 |
| a retrospective study of risk factors for carbapenem-resistant klebsiella pneumoniae acquisition among icu patients. | carbapenem-resistant klebsiella pneumoniae (crkp) is rapidly emerging as a life-threatening nosocomial infection. in this study, we aim to identify risk factors, especially antibiotic use, for crkp infection among intensive care unit (icu) patients. | 2016 | 27031451 |
| comparative effects of overproducing the arac-type transcriptional regulators mara, soxs, rara and rama on antimicrobial drug susceptibility in klebsiella pneumoniae. | in klebsiella pneumoniae, overproduction of rama and rara leads to increased mics of various antibiotics; mara and soxs are predicted to perform a similar function. we have compared the relative effects of overproducing these four arac-type regulators on envelope permeability (a combination of outer membrane permeability and efflux), efflux pump and porin production, and antibiotic susceptibility in k. pneumoniae. | 2016 | 27029850 |
| risk factors for kpc-producing klebsiella pneumoniae vap: keep an eye on previous therapies! | | 2016 | 27029825 |
| the klebsiella pneumoniae yfgl (bamb) lipoprotein contributes to outer membrane protein biogenesis, type-1 fimbriae expression, anti-phagocytosis, and in vivo virulence. | klebsiella pneumoniae is an opportunistic pathogen that causes several kinds of infections, including pneumonia, bacteremia, urinary tract infection and community-acquired pyogenic liver abscess (pla). adhesion is the critical first step in the infection process. our previous work demonstrated that the transcellular translocation is exploited by k. pneumoniae strains to migrate from the gut flora into other tissues, resulting in systemic infections. however, the initial stages of k. pneumoniae i ... | 2016 | 27029012 |
| evaluation of different phenotypic techniques for the detection of slime produced by bacteria isolated from clinical specimens. | introduction : microorganisms use various strategies for their survival in both the environment and in humans. slime production by bacteria is one such mechanism by which microbes colonize on the indwelling prosthetic devices and form biofilms. infections caused by such microorganisms are difficult to treat as the biofilm acts as a shield and protects microbes against antimicrobial agents. there are several methods for the detection of slime produced by bacteria, and they include both phenotypic ... | 2016 | 27026830 |
| recent research examining links among klebsiella pneumoniae from food, food animals, and human extraintestinal infections. | klebsiella pneumoniae is a colonizer of livestock, a contaminant of retail meats and vegetables, and a cause of extraintestinal infections in humans. antibiotic-resistant strains of k. pneumoniae are becoming increasingly prevalent among hospital and community-acquired infections. antibiotics are used extensively in conventional food-animal production, where they select for antibiotic-resistant bacteria. antibiotic-resistant k. pneumoniae has been isolated from livestock as well as from a variet ... | 2016 | 27022987 |
| [particularities of liver abscesses in children in senegal: description of a series of 26 cases]. | liver abscess is a serious infection that can cause life-threatening complications. | 2016 | 27021881 |
| can carbapenem-resistant enterobacteriaceae susceptibility results obtained from surveillance cultures predict the susceptibility of a clinical carbapenem-resistant enterobacteriaceae? | we evaluated the susceptibility profile of a colonizing carbapenem-resistant enterobacteriaceae to predict its susceptibility when recovered from a clinical specimen. an overall agreement of 88.7% (517 out of 583; 95% confidence interval, 85.8%-91.0%) was observed for the combinations of 11 antibiotics with 53 pairs of klebsiella pneumoniae carbapenemase-producing k pneumoniae (the only carbapenem-resistant enterobacteriaceae detected). very major errors were observed mainly for aminoglycoside a ... | 2016 | 27021509 |
| do we need new antibiotics? | for several years, alarmist articles both in mass media and in the scientific community have reported an increase in antibiotic resistance, even citing an inability to treat patients infected with multidrug-resistant bacteria (mdr) responsible for high mortality worldwide. in this review we summarize and discuss the key points associated with the reality of (i) the existence of pandrug-resistant bacteria, (ii) the increase of resistance worldwide, (iii) the link between resistance and death, and ... | 2016 | 27021418 |
| comparison of molecular and phenotypic methods for the detection and characterization of carbapenem resistant enterobacteriaceae. | in recent years, there has been a rapid dissemination of carbapenem resistant enterobacteriaceae (cre). this study aimed to compare phenotypic and molecular methods for detection and characterization of cre isolates at a large tertiary care hospital in saudi arabia. this study was carried out between january 2011 and november 2013 at the king khalid university hospital (kkuh) in saudi arabia. determination of presence of extended-spectrum beta-lactamases (esbl) and carbapenem resistance was in a ... | 2016 | 27020870 |
| susceptibility of klebsiella pneumoniae on coriander leaves to liquid- and vapor-phase ethanol. | the bio-control of ethanol on klebsiella pneumoniae on fresh coriander leaves for significantly reducing consumer health risk was investigated. washed and sterilized leaves of coriander were inoculated with k. pneumoniae cultured in trypticase soy broth. susceptibility of the k. pneumoniae to liquid- and evaporated vapor-phase ethanol (eve) was then examined in vitro complete inhibition of k. pneumoniae was found with 18% (v/v) liquid ethanol. exposure for 15 min to eve (9.00 ± 0.8 mmol l(-1)) c ... | 2016 | 27020413 |
| comparison of short versus prolonged infusion of standard dose of meropenem against carbapenemase-producing klebsiella pneumoniae isolates in different patient groups: a pharmacokinetic-pharmacodynamic approach. | dose optimization is required to increase carbapenem's efficacy against carbapenemase-producing isolates. four clinical klebsiella pneumoniae isolates were used: one susceptible to meropenem with minimum inhibitory concentration (mic) 0.031 mg/l and 3 verona integron-borne metallo bete-lactamase-1-producing isolates with mics 8, 16, and 128 mg/l. the human pharmacokinetics of short (0.5-h) and prolonged (3-h) infusion regimens of 1 g meropenem every 8 h were simulated in an in vitro pharmacokine ... | 2016 | 27019965 |
| [emphysematous cystitis and acute focal bacterial nephritis: a case report]. | a 96-year-old woman was diagnosed with colitis in our hospital. her past history was breast cancer and lung cancer but no diabetes mellitus. she suddenly complained of lower abdominal pain. abdominal computed tomography (ct) revealed intramural gas in the bladder wall and multiple poor contrasting area in her right kidney, which suggested emphysematous cystitis and acute focal bacterial nephritis. indwelling urethral catheter was performed for bladder drainage and the treatment with antibiotics ... | 2016 | 27018414 |
| inhibitory potential of buffalo (bubalus bubalis) colostrum immunoglobulin g on klebsiella pneumoniae. | the unique components of colostrum like free oligosaccharides and glycoconjugates are known to offer resistance to enzymatic digestion in the gastrointestinal tract and have the ability to inhibit the localized adherence of enteropathogens to the digestive tract of the neonates. in this context, we have evaluated the in vitro effect of buffalo colostrum immunoglobulin g on human pathogen klebsiella pneumoniae, a predominant multidrug resistant pathogen associated with nasocomial infections. the ... | 2016 | 27017977 |
| a case of hypermucoviscous klebsiella pneumoniae liver abscess syndrome in an iraqi male. | a 53-year-old man presented with fevers, productive cough and decreased appetite. he emigrated from iraq 4 years ago. chest x-ray revealed a left lung consolidation. respiratory cultures and two sets of blood cultures grew out pan-susceptible klebsiella pneumoniae liver ultrasound revealed a 6.4-cm complex lesion in the left hepatic lobe. a biopsy of the liver lesion produced bloody purulent aspirate; abscess cultures yielded a highly viscous pan-susceptible k. pneumoniae klebsiella pneumoniae l ... | 2016 | 27016535 |
| risk factors for colonization by carbapenemase-producing enterobacteria at admission to a surgical icu: a retrospective study. | in 2011, a hospital-wide outbreak of oxa-48 producing klebsiella pneumoniae occurred in our hospital, an epidemiological setting of high esbl-producing k. pneumoniae rates. this study identifies risk factors for colonization with carbapenemase-producing enterobacteria (cpe) at surgical intensive care unit (sicu) admission. | 2016 | 27016135 |
| screening of furanone in cucurbita melo and evaluation of its bioactive potential using in silico studies. | the work presented here attempts to screen for the presence of 4-hydroxy-2,5-dimethyl-3(2h)-furanone in fruits and also any bioactive potential present in the fruit extracts. curcurbita melo was selected for the study, and the fruit was crushed, filtered and extracted with ethyl acetate as the solvent. the resulting extract was subjected to disk diffusion test using kirby bauer method for checking its antimicrobial potential. melon extract showed promising results against different clinical path ... | 2016 | 27013507 |
| pediatric hypervirulent klebsiella pneumoniae septic arthritis. | cases of infection with hypervirulent klebsiella pneumoniae are gradually increasing in number, and cause life-threatening community-acquired infection even in immunocompetent patients. a 14-year-old boy developed septic hip arthritis due to hypervirulent k. pneumoniae (sequence type 23, serotype k1, maga positive). the patient initially seemed to have been successfully treated with antibiotics and surgical intervention, but septic arthritis developed into osteomyelitis of the femoral head and m ... | 2016 | 27005513 |
| carbapenemase-producing klebsiella pneumoniae bloodstream infections in neutropenic patients with haematological malignancies or aplastic anaemia: analysis of 50 cases. | carbapenemase-producing klebsiella pneumoniae (cp-kp) are currently among the most important nosocomial pathogens in many geographic regions. a retrospective study was conducted between 2010 and 2014 in four hospitals located in a high-prevalence area (athens, greece) to describe the clinical features, treatment and outcomes of neutropenic patients with haematological diseases complicated with cp-kp bloodstream infections. a total of 50 patients were identified, including 48 with haematological ... | 2016 | 27005460 |