| ceftazidime/avibactam: a novel cephalosporin/nonbeta-lactam beta-lactamase inhibitor for the treatment of complicated urinary tract infections and complicated intra-abdominal infections. | there has been greater interest in developing additional antimicrobial agents due to the increasing health care costs and resistance resulting from bacterial pathogens to currently available treatment options. gram-negative organisms including enterobacteriaceae and pseudomonas aeruginosa are some of the most concerning threats due to their resistance mechanisms: extended-spectrum beta-lactamase production and klebsiella pneumoniae carbapenemase enzymes. ceftazidime is a third-generation broad-s ... | 2016 | 27528799 |
| detection of blakpc and blages carbapenemase genes in klebsiella pneumoniae isolated from hospitalized patients in kashan, iran. | klebsiella pneumoniae carbapenemase (kpc)-producing bacteria are among the highly antimicrobial resistant gram negative bacteria and infections due to them are an increasingly major health problem worldwide. | 2016 | 27527726 |
| alterations in outer membrane permeability favor drug-resistant phenotype of klebsiella pneumoniae. | the increasing number of infections caused by multidrug-resistant and pandrug-resistant bacteria represents a serious worlwide problem. drug resistance limits available antimicrobials and can lead to suboptimal treatment of bacterial infections. it can be predicted that resistance to more antimicrobial drugs will be acquired by even more bacteria in the future. among the distinct resistance strategies, preventing drug entrance to intracellular compartment through modification of membrane permeab ... | 2016 | 27526080 |
| antimicrobial resistance among aerobic biofilm producing bacteria isolated from chronic wounds in the tertiary care hospitals of peshawar, pakistan. | chronic wound infections impose major medical and economic costs on health-care systems, cause significant morbidity, mortality and prolonged hospitalisation. the presence of biofilm producing bacteria in these wounds is considered as an important virulence factor that leads to chronic implications including ulceration. the undertaken study aimed to isolate and identify the biofilm aerobic bacterial pathogens from patients with chronic wound infections, and determine their antibiotics resistance ... | 2016 | 27523661 |
| reduced susceptibility of carbapenem-resistant klebsiella pneumoniae to biocides: an emerging threat. | dealing with carbapenem-resistant klebsiella pneumoniae (cr-kp) strains, which are generally pan-drug resistant, is an uphill task for health care professionals. owing to limited therapeutic options and the possibility of development of resistance to commonly used biocides in hospital settings, cr-kp infections pose a serious threat of emergence of a dreaded pandemic. the aim of the study was to highlight the possibility of emergence of biocide resistance among cr-kp strains. a case study was co ... | 2017 | 27514961 |
| klebseq, a diagnostic tool for surveillance, detection, and monitoring of klebsiella pneumoniae. | health care-acquired infections (hais) kill tens of thousands of people each year and add significantly to health care costs. multidrug-resistant and epidemic strains are a large proportion of hai agents, and multidrug-resistant strains of klebsiella pneumoniae, a leading hai agent, have caused an urgent public health crisis. in the health care environment, patient colonization by k. pneumoniae precedes infection, and transmission via colonization leads to outbreaks. periodic patient screening f ... | 2016 | 27510832 |
| endotipsitis caused by extremely drug-resistant klebsiella pneumoniae. | | 2016 | 27509714 |
| acinetobacter spp. are associated with a higher mortality in intensive care patients with bacteremia: a survival analysis. | it has been challenging to determine the true clinical impact of acinetobacter spp., due to the predilection of this pathogen to colonize and infect critically ill patients, who often have a poor prognosis. the aim of this study was to assess whether acinetobacter spp. bacteremia is associated with lower survival compared with bacteremia caused by other pathogens in critically ill patients. | 2016 | 27506948 |
| invasive liver abscess syndrome predisposed by klebsiella pneumoniae related prostate abscess in a nondiabetic patient: a case report. | prostate abscess is usually a complication of acute urinary tract infection. invasive liver abscess syndrome is characterized with klebsiella pneumoniae related multiple organ metastasis. concomitant pyogenic liver abscess and prostate abscess have rarely been reported. recurrent episode of liver abscess is even rarer. | 2016 | 27506523 |
| influence of camp receptor protein (crp) on bacterial virulence and transcriptional regulation of alls by crp in klebsiella pneumoniae. | camp receptor protein (crp) is one of the most important transcriptional regulators, which can regulate large quantities of operons in different bacteria. the gene alls was well-known as allantoin-utilizing capability and involving in bacterial virulence in klebsiella pneumoniae (k. pneumoniae). the specific dna recognition motif of transcription regulator crp was found in alls promoter region. therefore, this study is aimed to investigate the function of crp on virulence and its transcriptional ... | 2016 | 27502416 |
| synergistic effects of baicalein with cefotaxime against klebsiella pneumoniae through inhibiting ctx-m-1 gene expression. | generation of extended- spectrum β- lactamases is one of the major mechanisms by which clinical klebsiella pneumoniae develop resistance to antibiotics. combined antibiotics prove to be a relatively effective method of controlling such resistant strains. some of chinese herbal active ingredients are known to have synergistic antibacterial effects. this study is aimed to investigate synergistic effects of chinese herbal active ingredients with cefotaxime on the extended- spectrum β- lactamase pos ... | 2016 | 27502110 |
| association between presence of virulence genes and antibiotic resistance in clinical klebsiella pneumoniae isolates. | to investigate the presence of rmpa and wcag virulence genes and class 1, 2, and 3 integrons, and to evaluate a relationship between antibiotic resistance and virulence in klebsiella pneumoniae methods: we collected a total of 200 k. pneumoniae isolates from hospitals in tehran, iran. antibiotic susceptibility was determined using the disk diffusion method. the extended-spectrum β-lactamase (esbl) producers were detected using the combination disk method. we detected the rmpa and wcag genes and ... | 2016 | 27498999 |
| in vivo efficacy of single phage versus phage cocktail in resolving burn wound infection in balb/c mice. | klebsiella pneumoniae is one of the most predominant pathogens associated with burn wound infections, causing considerable morbidity and mortality. the indiscriminate usage of antibiotics has led to the development of resistant strains, which have contributed towards the inefficacy of antibiotics. phage therapy is a promising alternative to hinder the progression of pathogenic bacteria. however, phage bacterial resistance is already well known but the use of phage cocktails can overcome this dra ... | 2016 | 27498362 |
| successful treatment of urinary tract infection in kidney transplant recipients caused by multiresistant klebsiella pneumoniae producing new delhi metallo-beta-lactamase (ndm-1) with strains genotyping. | klebsiella pneumoniae new delhi metallo-beta-lactamase-1 (ndm-1) strains have recently become a new threat in kidney transplant recipients due to the strains' resistance to almost all antibiotics, including carbapenems. | 2016 | 27496450 |
| oral decontamination with aminoglycosides is associated with lower risk of mortality and infections in high-risk patients colonized with colistin-resistant, kpc-producing klebsiella pneumoniae. | invasive infections caused by kpc-producing klebsiella pneumoniae (kpckp) are associated with very high mortality. because infection is usually preceded by rectal colonization, we investigated if decolonization therapy (dt) with aminoglycosides had a protective effect in selected patients. | 2016 | 27494911 |
| nosocomial outbreak of oxa-48-producing klebsiella pneumoniae in a chinese hospital: clonal transmission of st147 and st383. | in china, the spread and outbreak of oxa-48-producing enterobacteriaceae remains largely unknown. | 2016 | 27490695 |
| klebsiella pneumoniae bloodstream infection, antimicrobial resistance and consumption trends in ireland: 2008 to 2013. | this study aimed to describe the epidemiology and antimicrobial resistance trends of klebsiella pneumoniae bloodstream infection (bsi) in ireland, in conjunction with national antimicrobial consumption data, during the period 2008 to 2013. a retrospective cohort study of k. pneumoniae bsi cases was conducted, based on notifications from irish microbiology laboratories to the health protection surveillance centre (hpsc). in total, 1942 k. pneumoniae bsi cases were identified over 6 years, with 31 ... | 2016 | 27488438 |
| high prevalence of hypervirulent klebsiella pneumoniae infection in china: geographic distribution, clinical characteristics, and antimicrobial resistance. | hypervirulent klebsiella pneumoniae (hvkp) is traditionally defined by hypermucoviscosity, but data based on genetic background are limited. antimicrobial-resistant hvkp has been increasingly reported but has not yet been systematically studied. k. pneumoniae isolates from bloodstream infections, hospital-acquired pneumonia, and intra-abdominal infections were collected from 10 cities in china during february to july 2013. clinical data were collected from medical records. all k. pneumoniae isol ... | 2016 | 27480857 |
| genetic diversity of klebsiella pneumoniae isolates during an outbreak in a non-human primate research colony. | klebsiella pneumoniae can be a serious pathogen in non-human primates, particularly neotropical monkeys. | 2016 | 27477611 |
| bloodstream infections caused by klebsiella pneumoniae carbapenemase 2-producing k. pneumoniae at a hematology department in wenzhou, china. | the increasing prevalence of klebsiella pneumoniae carbapenemase 2-producing k. pneumoniae (kpc-2-kp) infections can become a new life-threatening complication for hematological patients. five cases of kpc-2-kp bloodstream infections have been identified in our hematology department over the past 10 years. the current treatment options do not show satisfactory efficacy, especially for bloodstream infections. the treatment of these five cases was unsuccessful, mainly due to the high minimum inhib ... | 2016 | 27477421 |
| molecular signature of extended spectrum β-lactamase producing klebsiella pneumoniae isolated from bovine milk in eastern and north-eastern india. | the present study reports on 23 extended spectrum β-lactamase producing klebsiella pneumoniae (kp), isolated from milk samples (n=340) of healthy cows (n=129) and cows with subclinical (n=159) and clinical (n=52) mastitis, from three different states of india viz. west bengal, jharkhand and mizoram. seven of them were ampc type β-lactamase producers, as well. the esbl producing kp were significantly (p=0.006, χ2=10.04, df=2) and more frequently detected in milk samples of mastitic cows than heal ... | 2016 | 27473782 |
| carbapenem-resistant enterobacteriaceae in special populations: solid organ transplant recipients, stem cell transplant recipients, and patients with hematologic malignancies. | carbapenem-resistant enterobacteriaceae (cre) are a major global public health concern and pose a serious threat to immunocompromised hosts, particularly patients with hematologic malignancies and solid organ (sot) and stem cell transplant recipients. in endemic areas, carbapenem-resistant klebsiella pneumoniae infections occur in 1-18% of sot recipients, and patients with hematologic malignancies represent 16-24% of all patients with cre bacteremia. mortality rates approaching 60% have been rep ... | 2016 | 27470662 |
| [rapid increase in resistance to third generation cephalosporins, imipenem and co-resistance in klebsiella pneumoniae from isolated from 7,140 blood-cultures (2010-2014) using ears-net data in spain]. | an analysis was made about the evolution of resistance to 3rd generation cephalosporins, imipenem, and other antibiotics in invasive isolates of klebsiella pneumoniae (k. pneumoniae) according to the spanish ears-net database (2010-2014). | 2016 | 27469416 |
| emergence and interhospital spread of oxa-48-producing klebsiella pneumoniae st395 clone in western hungary. | | 2017 | 27465499 |
| evaluation of antibacterial effects of zataria multiflora boiss extracts against esbl-producing klebsiella pneumoniae strains. | there are few therapeutic options for treatment of multidrug resistant klebsiella pneumoniae isolates as a hospital infectious agent (nosocomial infection). the aim of this study was to evaluate the antibacterial activity of zataria multiflora boiss extracts against esbl-producing klebsiella pneumoniae strains. | 2017 | 27462557 |
| in vitro activity of tigecycline in combination with rifampin, doripenem or ceftazidime against carbapenem-resistant klebsiella pneumoniae bloodstream isolates. | | 2017 | 27460764 |
| knockdown of hmgn2 increases the internalization of klebsiella pneumoniae by respiratory epithelial cells through the regulation of α5β1 integrin expression. | integrin receptors, a large family of adhesion receptors, are involved in the attachment of klebsiella pneumoniae to respiratory epithelial cells, and subsequently cause the internalization of k. pneumoniae by host cells. although a number of molecules have been reported to regulate the expression and activity of integrin receptors in respiratory epithelial cells, the specific underlying molecular mechanisms remain largely unknown. high mobility group nucleosomal binding domain 2 (hmgn2), a non- ... | 2016 | 27460641 |
| endogenous endophthalmitis in the american and korean population: an 8-year retrospective study. | to study the clinical features of endogenous endophthalmitis (ee) in sample patient populations from the usa and south korea over an 8-year period. | 2016 | 27459423 |
| successful treatment of a disseminated infection with extensively drug-resistant klebsiella pneumoniae in a liver transplant recipient with a fosfomycin-based multidrug regimen. | donor-derived infections with multidrug-resistant gram-negative bacteria are associated with poor outcomes, in part because of limited treatment options. here, we describe a case of donor-derived, disseminated infection with colistin-resistant, carbapenemase-producing klebsiella pneumoniae in a liver transplant recipient that was cured with addition of intravenous fosfomycin to a multidrug regimen, in conjunction with aggressive surgical source control. intravenous fosfomycin represents a promis ... | 2016 | 27458980 |
| performance of four different agar plate methods for rectal swabs, synergy disk tests and metallo-β-lactamase etest for clinical isolates in detecting carbapenemase-producing klebsiella pneumoniae. | the aims of the study were to compare four different agar plate methods in the identification of carbapenemase-producing klebsiella pneumoniae (cp-kp) from rectal samples and to assess the role of phenotypic methodologies in the identification of carbapenemase type from clinical k. pneumoniae isolates. two chromogenic agars (brilliance cre and chromagar kpc) were compared to macconkey agar plates with ertapenem (ert) or imipenem (imp) disks for the identification of cp-kp from 912 rectal swabs. ... | 2016 | 27452448 |
| phenotypic and molecular characterization of antimicrobial resistance in klebsiella spp. isolates from companion animals in japan: clonal dissemination of multidrug-resistant extended-spectrum β-lactamase-producing klebsiella pneumoniae. | the emergence of antimicrobial resistance in klebsiella spp., including resistance to extended-spectrum cephalosporins (esc) and fluoroquinolones, is of great concern in both human and veterinary medicine. in this study, we investigated the prevalence of antimicrobial resistance in a total of 103 klebsiella spp. isolates, consisting of klebsiella pneumoniae complex (kp, n = 89) and k. oxytoca (ko, n = 14) from clinical specimens of dogs and cats in japan. furthermore, we characterized the resist ... | 2016 | 27446056 |
| kpc-3-producing klebsiella pneumoniae in portugal linked to previously circulating non-cg258 lineages and uncommon genetic platforms (tn4401d-incfia and tn4401d-incn). | kpc-3-producing bacteria are endemic in many countries but only recently became apparent their wide distribution in different portuguese hospitals. the aim of this study is to characterize genetic backgrounds associated with bla kpc-3 among klebsiella pneumoniae isolates recently identified on non-hospitalized patients in portugal. twenty kpc-producing k. pneumoniae identified between october 2014 and november 2015 in three different community laboratories were characterized. isolates were mainl ... | 2016 | 27446040 |
| persistent cutaneous abdominal ulcerations secondary to diffuse dermal angiomatosis: an underestimated sign for severe atherosclerosis: a case report. | diffuse dermal angiomatosis (dda) is a rare, acquired, reactive vascular proliferation, clinically characterized by livedoid erythematous-violaceous plaques, which frequently evolve to ulceration and necrosis. histopathologically, it is manifested by a diffuse proliferation of endothelial cells within the full thickness of the dermis. dda has been mainly associated with severe peripheral atherosclerosis. | 2016 | 27442644 |
| adaptive laboratory evolution of klebsiella pneumoniae for improving 2,3-butanediol production. | microbial production of 2,3-butanediol is limited by the toxic components in the lignocellulose hydrolysate. to improve the 2,3-butanediol production via klebsiella pneumoniae from cotton stalk hydrolysate, a method coupling a high tolerance of strain and detoxification of the hydrolysate was thus investigated in this study. the strain tolerance of k. pneumoniae to the cotton stalk hydrolysate was improved via an adaptive laboratory evolution, which involved a stepwise increase in the hydrolysat ... | 2016 | 27442598 |
| epidemiology of carbapenem resistant klebsiella pneumoniae infections in mediterranean countries. | infections by carbapenem-resistant enterobacteriaceae (cre), in particular, carbapenem-resistant klebsiella pneumoniae (crkp), are a significant public health challenge worldwide. resistance to carbapenems in enterobacteriaceae is linked to different mechanisms, including the production of the various types of enzymes like kpc, vim, imp, ndm, and oxa-48. despite several attempts to control the spread of these infections at the local and national level, the epidemiological situation for crkp had ... | 2016 | 27441063 |
| the role of budabc on 1,3-propanediol and 2,3-butanediol production from glycerol in klebsiella pneumoniae cicim b0057. | 1,3-propanediol (1,3-pd) is an important compound from which many others can be synthesized. 2,3-butanediol (bdo) is the key by-product in the biosynthesis of 1,3-pd from glycerol, but it impedes its downstream purification. in klebsiella, the buda, budb and budc genes encode enzymes that are responsible for the synthesis of bdo. in this study, 3 individual antisense rnas were designed to repress the expression and hence activity of buda-c. compared with the parent strains, the activities of bud ... | 2016 | 27439015 |
| klebsiella pneumoniae esbl forming spheroplasts in the fresh and unstained urine sediment. | a 60 year-old man was submitted to kidney transplantation in 2013 due to chronic renal insufficiency caused by hypertension. he had recurrent episodes of urinary tract infection and came to the hospital due to a 4 day-long fever, abdominal pain, burning urination and nausea. routine urinalysis revealed a picture of infection (> 50 leucocytes/high power field associated to massive bacteriuria). the urine sediment revealed elongated like elements with an enlarged part in the middle of the structur ... | 2016 | 27438984 |
| klebsiella pneumoniae infection leads to a poor visual outcome in endogenous endophthalmitis: a 12-year experience in southern taiwan. | to compare the characteristics, visual outcome, and prognostic factors of patients with endogenous endophthalmitis and to determine the association of endophthalmitis with klebsiella pneumoniae infection. | 2016 | 27438458 |
| carriage of multidrug resistant bacteria on frequently contacted surfaces and hands of health care workers. | maximal contact between the patients and health care workers (hcws) happens in the intensive care units (icu). control of nosocomial infections requires compliance with hand hygiene and contamination free surfaces. | 2016 | 27437214 |
| molecular typing and genetic environment of the blakpc gene in chilean isolates of klebsiella pneumoniae. | the aim of this work was to determine the genetic environment and transferability of blakpc as well as the pulsotypes of kpc-producing klebsiella pneumoniae strains isolated from clinical samples in chilean hospitals. seventeen strains, principally isolated in santiago (the capital of chile) during the years 2012 and 2013, were included. the genetic environment of blakpc was elucidated by pcr mapping and sequencing. molecular typing was performed by pulsed-field gel electrophoresis (pfge) and mu ... | 2016 | 27436389 |
| spotlight on ceftazidime/avibactam: a new option for mdr gram-negative infections. | during the last decade infections caused by mdr gram-negative bacteria (gnb) have become increasingly prevalent. because of their high morbidity and mortality rates, these infections constitute a serious threat to public health worldwide. ceftazidime/avibactam is a new approved agent combining ceftazidime and a novel β-lactamase inhibitor with activity against various β-lactamases produced by mdr gnb. avibactam has a spectrum of inhibition of class a and c β-lactamases, including esbls, ampc and ... | 2016 | 27432599 |
| immunotherapies against antibiotics-resistant klebsiella pneumoniae. | | 2016 | 27431874 |
| emergence and plasmid analysis of klebsiella pneumoniae kp01 carrying blages-5 from guangzhou, china. | klebsiella pneumoniae strain kp01 carrying blages-5 was identified from a patient in guangzhou, china. high-throughput sequencing assigned blages-5 to a 28.5-kb nonconjugative plasmid, pges-gz. a 13-kb plasmid backbone sequence on pges-gz was found to share high sequence identities with plasmids from gram-negative nonfermenters. a novel class 1 integron carrying a gene cassette array of orf28-orf28-blages-5 was identified on pges-gz, within which orf28 encoded a hypothetical protein possibly cor ... | 2016 | 27431225 |
| meropenem for treating kpc-producing klebsiella pneumoniae bloodstream infections: should we get to the pk/pd root of the paradox? | the objective of this study was to assess the achievement of pharmacokinetic/pharmacodynamic (pk/pd) targets of meropenem (mem) in critically-ill patients with bloodstream infections (bsi) due to klebsiella pneumoniae-carbapenemase-producing klebsiella pneumoniae (kpc-kp) with mem minimum inhibitory concentrations (mics) ≥16 mg/l. nineteen critically-ill patients with kpc-kp bsi were given combination therapy including mem, tigecycline, plus colistin or gentamicin (according to susceptibility te ... | 2017 | 27430122 |
| hemodialysis catheter-related infection: rates, risk factors and pathogens. | the main complication of central venous catheter (cvc) in hemodialysis is infection. identifying cvc related infection (cvc-ri) risk factors and causative micro-organisms is important for setting prevention policies. there were no data regarding cvc-ri in hemodialysis in algeria. to determine rates of cvc-ri in hemodialysis in setif university hospital, risk factors and causative microorganisms, we conducted a prospective study from november 2014 to may 2015 involving patients with cvc in hemodi ... | 2016 | 27423929 |
| detection of new delhi metallo-beta-lactamase-1 (ndm-1) in carbapenem- resistant klebsiella pneumoniae isolated from a university hospital in iran. | new delhi metallo-beta-lactamase-1(ndm-1) is a novel type of metallo-beta-lactamase (mbl) which inactivates all β-lactam antibiotics except aztreonam. enterobacteriaceae expressing ndm-1 have been identified worldwide. the aim of this study was to detect mbls in carbapenem-resistant k. pneumoniae isolates obtained from patients hospitalized in one of the university hospitals in isfahan, iran. | 2017 | 27418777 |
| complete genome sequence of a klebsiella pneumoniae strain carrying blandm-1 on a multidrug resistance plasmid. | here, we report the genome sequence of a blandm-1-positive klebsiella pneumoniae aatzp isolate cultured from a perirectal surveillance swab collected upon admission of a patient to the nih clinical center in 2014. genome sequencing of this isolate revealed three plasmids, including one carrying the blandm-1 gene encoding resistance to carbapenems. | 2016 | 27417839 |
| evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of kpc-producing klebsiella pneumoniae. | we evaluated a real-time single-peak (11.109-da) detection assay based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) for the identification of klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae our results demonstrated that the 11.109-da peak was detected in 88.2% of the kpc producers. analysis of blakpc-producing k. pneumoniae showed that the gene encoding the 11.109-da protein was commonly (97.8%) associated with the tn4401a isofo ... | 2016 | 27413192 |
| glycerol-fed microbial fuel cell with a co-culture of shewanella oneidensis mr-1 and klebsiella pneumonae j2b. | glycerol is an attractive feedstock for bioenergy and bioconversion processes but its use in microbial fuel cells (mfcs) for electrical energy recovery has not been investigated extensively. this study compared the glycerol uptake and electricity generation of a co-culture of shewanella oneidensis mr-1 and klebsiella pneumonia j2b in a mfc with that of a single species inoculated counterpart. glycerol was metabolized successfully in the co-culture mfc (mfc-j&m) with simultaneous electricity prod ... | 2016 | 27412724 |
| improved production of tannase by klebsiella pneumoniae using indian gooseberry leaves under submerged fermentation using taguchi approach. | tannase (tannin acyl hydrolase e.c 3.1.1.20) is an inducible, largely extracellular enzyme that causes the hydrolysis of ester and depside bonds present in various substrates. large scale industrial application of this enzyme is very limited owing to its high production costs. in the present study, cost effective production of tannase by klebsiella pneumoniae kp715242 was studied under submerged fermentation using different tannin rich agro-residues like indian gooseberry leaves (phyllanthus emb ... | 2016 | 27411334 |
| differential host susceptibility and bacterial virulence factors driving klebsiella liver abscess in an ethnically diverse population. | hypervirulent klebsiella pneumoniae is an emerging cause of community-acquired pyogenic liver abscess. first described in asia, it is now increasingly recognized in western countries, commonly afflicting those with asian descent. this raises the question of genetic predisposition versus geospecific strain acquisition. we leveraged on the antibiotics for klebsiella liver abscess syndrome study (a-klass) clinical trial ongoing in ethnically diverse singapore, to prospectively examine the profiles ... | 2016 | 27406977 |
| need for lumbar puncture in children younger than 12 months presenting with simple febrile seizure. | recently, conflicting results have been reported regarding the necessity of routine lumbar puncture in children less than 12 months of age with simple febrile seizure. the aims of this study were to evaluate the results of lumbar puncture in children younger than 60 months of age with febrile seizure and to reassess the need for lumbar puncture in children younger than 12 months with simple febrile seizure. | 2016 | 27404463 |
| primary osteomyelitis caused by hypervirulent klebsiella pneumoniae. | klebsiella pneumoniae is the most clinically relevant species of this genus, known to cause both community-acquired and nosocomial infections worldwide. in the past two decades, a distinct hypervirulent strain of k pneumoniae, characterised by its hypermucoviscous phenotype, has emerged as a clinically significant pathogen responsible for highly invasive infections. we present a case of osteomyelitis due to hypervirulent k pneumoniae reported in the usa. genomic testing of the k pneumoniae isola ... | 2016 | 27402393 |
| combination treatment with extended-infusion ceftazidime/avibactam for a kpc-3-producing klebsiella pneumoniae bacteraemia in a kidney and pancreas transplant patient. | | 2016 | 27396948 |
| validation of minim typing for fast and accurate discrimination of extended-spectrum, beta-lactamase-producing klebsiella pneumoniae isolates in tertiary care hospital. | minim typing is derived from the multi-locus sequence typing (mlst). it targets the same genes, but sequencing is replaced by high resolution melt analysis. typing can be performed by analysing six loci (6melt), four loci (4melt) or using data from four loci plus sequencing the tonb gene (hybridmelt). the aim of this study was to evaluate minim typing to discriminate extended-spectrum beta-lactamase producing klebsiella pneumoniae (esbl-klpn) isolates at our hospital. in total, 380 isolates were ... | 2016 | 27394639 |
| prevalence of hypervirulent klebsiella pneumoniae-associated genes rmpa and maga in two tertiary hospitals in houston, tx, usa. | | 2016 | 27392968 |
| dnat is a pric-binding protein. | dnat and pric are replication restart primosomal proteins required for re-initiating chromosomal dna replication. dnat is a component of the pria-dependent primosome, while pric belongs to the pric-dependent primosome. whether dnat can interact with pric is still unknown. in this study, we define a direct interaction between pric, a key initiator protein in pric-mediated dna replication restart, and dnat, a dnab/c complex loader protein, from klebsiella pneumoniae. in fluorescence titrations, pr ... | 2016 | 27387236 |
| effect of the β-lactamase inhibitor vaborbactam combined with meropenem against serine carbapenemase-producing enterobacteriaceae. | klebsiella pneumoniae carbapenemase (kpc)-producing isolates have become increasingly prevalent worldwide, and these organisms are often multidrug resistant, limiting the therapeutic options available for treating infections. we evaluated the activity of meropenem combined with the serine β-lactamase inhibitor vaborbactam (formerly rpx7009) against 315 serine carbapenemase-producing enterobacteriaceae (cpe) isolates by use of checkerboard-designed panels to assess the optimal inhibitor concentra ... | 2016 | 27381386 |
| impacts of hypervirulence determinants on clinical features and outcomes of bacteremia caused by extended-spectrum β-lactamase-producing klebsiella pneumoniae. | we investigated the implications of hypervirulence determinants on clinical features of 48 adult patients with bacteremia caused by extended-spectrum β-lactamase-producing klebsiella pneumoniae. isolates in the hypervirulence group included any of the following virulence determinants: k1/k2 capsule serotypes, hypermucoviscosity phenotype, rmpa gene, or rmpa2 gene. nonhypervirulence group isolates were negative for all of the above virulence factors. in this study, all isolates used were non-k1/k ... | 2017 | 27380450 |
| experimental single-strain mobilomics reveals events that shape pathogen emergence. | virulence genes on mobile dnas such as genomic islands (gis) and plasmids promote bacterial pathogen emergence. excision is an early step in gi mobilization, producing a circular gi and a deletion site in the chromosome; circular forms are also known for some bacterial insertion sequences (iss). the recombinant sequence at the junctions of such circles and deletions can be detected sensitively in high-throughput sequencing data, using new computational methods that enable empirical discovery of ... | 2016 | 27378783 |
| efficient transmission of incfiiy and incl plasmids and klebsiella pneumoniae st101 clone producing oxa-48, ndm-1 or oxa-181 in bucharest hospitals. | | 2016 | 27378198 |
| lactobacillus salivarius reverse diabetes-induced intestinal defense impairment in mice through non-defensin protein. | altered intestinal microbiota and subsequent endotoxemia play pathogenic roles in diabetes. we aimed to study the mechanisms of intestinal defense impairment in type 1 diabetes and the effects of lactobacillus salivarius as well as fructooligosaccharides (fos) supplementation on diabetes-induced bacterial translocation. alterations in the enteric microbiome, expression of mucosal antibacterial proteins and bacteria-killing activity of the intestinal mucosa in streptozotocin (stz)-induced diabeti ... | 2016 | 27376728 |
| decontamination of indoor air to reduce the risk of airborne infections: studies on survival and inactivation of airborne pathogens using an aerobiology chamber. | although indoor air can spread many pathogens, information on the airborne survival and inactivation of such pathogens remains sparse. | 2016 | 27375064 |
| countrywide dissemination of a dha-1-type plasmid-mediated ampc β-lactamase-producing klebsiella pneumoniae st11 international high-risk clone in hungary, 2009-2013. | the first plasmid-mediated ampc β-lactamase-producing klebsiella pneumoniae (pampc kp) isolate was detected in december 2009 in hungary. hungarian microbiological laboratories were asked to send all kp strains showing cefoxitin resistance and decreased susceptibility or resistance to any third-generation cephalosporins to the reference laboratories at the national center for epidemiology. investigation was conducted in order to outline spatio-temporal distribution and genetic characterization of ... | 2016 | 27375036 |
| [impact of extended-spectrum β-lactamase on clinical outcome and medical cost in patients with bloodstream infection due to klebsiella pneumoniae]. | to evaluate the impact of extended-spectrum β-lactamase (esbl) on clinical outcome and medical cost in patients with bloodstream infection (bsi) due to klebsiella pneumoniae. | 2016 | 27373357 |
| risk factors for infections due to carbapenem-resistant klebsiella pneumoniae after open heart surgery. | patients undergoing major surgery are at increased risk of developing infections due to resistant organisms, including carbapenem-resistant klebsiella pneumoniae (cr-kp). in this study, we assessed risk factors for cr-kp infections after open heart surgery in a teaching hospital in northern italy. | 2016 | 27371609 |
| boronic acid disk diffusion for the phenotypic detection of polymerase chain reaction-confirmed, carbapenem-resistant, gram-negative bacilli isolates. | the middle east is regarded as a secondary reservoir for oxa-48 and new delhi metallo-β-lactamase (ndm) carbapenemases. one of the main challenges in clinical microbiology diagnostics is the detection of carbapenemases. for this reason simple screening methods have been sought to detect carbapenemase producers to determine appropriate therapeutic measures and implement infection control interventions. the present study aimed to evaluate the efficacy of the modified hodge test (mht) and a boronic ... | 2016 | 27368987 |
| antibacterial constituents of fructus chebulae immaturus and their mechanisms of action. | to extract, purify, and identify the effective constituents of aqueous extract of fructus chebulae immaturus, and analyze the bactericidal effects of total tannins. | 2016 | 27368700 |
| complement resistance mechanisms of klebsiella pneumoniae. | the current emergence of antibiotic-resistant bacteria causes major problems in hospitals worldwide. to survive within the host, bacterial pathogens exploit several escape mechanisms to prevent detection and killing by the immune system. as a major player in immune defense, the complement system recognizes and destroys bacteria via different effector mechanisms. the complement system can label bacteria for phagocytosis or directly kill gram-negative bacteria via insertion of a pore-forming compl ... | 2016 | 27364766 |
| rise and fall of kpc-producing klebsiella pneumoniae in new york city. | the study objective was to examine the epidemiological trends of kpc-producing klebsiella pneumoniae in new york city medical centres. | 2016 | 27353464 |
| [distribution of pathogenic bacteria and its influence on expression of bcl-2 and bax protein after hsct in the patients with hematological malignancies]. | to investigate the distribution of pathogenic bacteria in the patients with hematologic malignancies received hematopoietic stem cell transplantation (hsct) and its influence on the expression of bcl-2 and bax proteins. | 2016 | 27342520 |
| deletion of ldha and aldh genes in klebsiella pneumoniae to enhance 1,3-propanediol production. | to improve 1,3-propanediol (1,3-pd) production and reduce byproduct concentration during the fermentation of klebsiella pneumonia. | 2016 | 27341834 |
| complete genome sequence of the first kpc-type carbapenemase-positive proteus mirabilis strain from a bloodstream infection. | sequencing of the blakpc-positive strain proteus mirabilis aouc-001 was performed using both the miseq and pacbio rs ii platforms and yielded a single molecule of 4,272,433 bp, representing the complete chromosome. genome analysis showed the presence of several acquired resistance determinants, including two copies of blakpc-2 carried on a fragment of a kpc-producing plasmid previously described in klebsiella pneumoniae. | 2016 | 27340072 |
| gluconic acid production by gad mutant of klebsiella pneumoniae. | klebsiella pneumoniae produces many economically important chemicals. using glucose as a carbon source, the main metabolic product in k. pneumoniae is 2,3-butanediol. gluconic acid is an intermediate of the glucose oxidation pathway. in the current study, a metabolic engineering strategy was used to develop a gluconic acid-producing k. pneumoniae strain. deletion of gad, resulting in loss of gluconate dehydrogenase activity, led to the accumulation of gluconic acid in the culture broth. gluconic ... | 2016 | 27339313 |
| lactobacillus casei triggers a tlr mediated rack-1 dependent p38 mapk pathway in caenorhabditis elegans to resist klebsiella pneumoniae infection. | in the present study, the effect of lactic acid bacteria (lab) was investigated at the molecular level using the model organism caenorhabditis elegans against klebsiella pneumoniae. out of the 13 lab screened, lactobacillus casei displayed excellent protective efficacy by prolonging the survival of k. pneumoniae-infected nematodes. pretreatment with l. casei significantly decreased bacterial colonization and rescued k. pneumoniae-infected c. elegans from various physiological impairments. the co ... | 2016 | 27338631 |
| mobilization of carbapenemase-mediated resistance in enterobacteriaceae. | there has been a dramatic increase in the last decade in the number of carbapenem-resistant enterobacteriaceae, often leaving patients and their providers with few treatment options and resultant poor outcomes when an infection develops. the majority of the carbapenem resistance is mediated by bacterial acquisition of one of three carbapenemases (klebsiella pneumoniae carbapenemase [kpc], oxacillinase-48-like [oxa-48], and the new delhi metallo-β-lactamase [ndm]). each of these enzymes has a uni ... | 2016 | 27337454 |
| evaluation of antibacterial activities of colistin, rifampicin and meropenem combinations against ndm-1-producing klebsiella pneumoniae in 24 h in vitro time-kill experiments. | to investigate the activity of colistin alone or in double and triple combination with rifampicin and meropenem against ndm-1-producing klebsiella pneumoniae. | 2016 | 27334665 |
| first report of a clinical isolate of new delhi metallo-β-lactamase-producing klebsiella pneumoniae in brazil. | | 2016 | 27328614 |
| emergence and characterization of tigecycline resistance in multidrug-resistant klebsiella pneumoniae isolates from blood samples of patients in intensive care units in northern china. | serious infections in intensive care unit patients caused by multidrug-resistant (mdr) klebsiella pneumoniae represent a major threat worldwide owing to increased mortality and limited treatment options. with the application of tigecycline for mdr pathogens, tigecycline-non-susceptible k. pneumoniae isolates have recently emerged in china. to identify the susceptibility profile of mdr k. pneumoniae to tigecycline and evaluate the molecular characterization of tigecycline resistance, 214 mdr k. p ... | 2016 | 27324378 |
| biofilm inhibitory effect of chlorhexidine conjugated gold nanoparticles against klebsiella pneumoniae. | klebsiella pneumoniae (k. pneumoniae) is one of the major pathogen associated with nosocomial infections, especially catheter associated urinary tract infections which involved biofilm formation. this study was designed to evaluate the antibiofilm efficacy of gold nanoparticle conjugated with chlorhexidine (au-chx) against k. pneumoniae isolates. au-chx was synthesized and analyzed for stability by using uv-visible spectrophotometry, atomic force microscopy (afm), fourier transform infrared spec ... | 2016 | 27321770 |
| solar photocatalysis as disinfection technique: inactivation of klebsiella pneumoniae in sewage and investigation of changes in antibiotic resistance profile. | the presence of pathogenic microorganisms in wastewater and their resistant nature to antibiotics impose effective disinfection treatment for public health and environmental protection. in this work, photocatalysis with metal-doped titania under artificial and natural sunlight, chlorination and uv-c irradiation were evaluated for their potential to inactivate klebsiella pneumoniae in real wastewater. their overall effect on antibiotic resistance profile and target antibiotic resistance genes (ar ... | 2016 | 27316624 |
| comparison of six commercial kits to extract bacterial chromosome and plasmid dna for miseq sequencing. | we compared commercial kits for extraction of genomic dna from the gram-negative bacterium klebsiella pneumoniae for subsequent miseq sequencing. purification of dna was based on matrix binding (silica or anion exchange resin) or differential precipitation (salting out), respectively. the choice of extraction kit had little effect on sequencing quality and coverage across drastically different replicons, except for an apparent depletion of small plasmids (<5 kb) during precipitation-based extrac ... | 2016 | 27312200 |
| klebsiella pneumoniae: going on the offense with a strong defense. | klebsiella pneumoniae causes a wide range of infections, including pneumonias, urinary tract infections, bacteremias, and liver abscesses. historically, k. pneumoniae has caused serious infection primarily in immunocompromised individuals, but the recent emergence and spread of hypervirulent strains have broadened the number of people susceptible to infections to include those who are healthy and immunosufficient. furthermore, k. pneumoniae strains have become increasingly resistant to antibioti ... | 2016 | 27307579 |
| epidemiology of carbapenem-resistant klebsiella pneumoniae colonization: a surveillance study at a turkish university hospital from 2009 to 2013. | between june 2009 and december 2013, 4105 patients were screened for carbapenem-resistant klebsiella pneumoniae (cr-kp) colonization in a tertiary care university hospital. the antimicrobial susceptibility and resistance determinants of 279 (6.8%) cr-kp isolates from single patients were investigated. additional analysis was performed to evaluate the characteristics and various risk factors for infection in patients with colonization. of the 279 isolates, 270 harboured oxa-48-like enzymes, and a ... | 2016 | 27306118 |
| biochemical and structural characterization of klebsiella pneumoniae oxamate amidohydrolase in the uric acid degradation pathway. | hpxw from the ubiquitous pathogen klebsiella pneumoniae is involved in a novel uric acid degradation pathway downstream from the formation of oxalurate. specifically, hpxw is an oxamate amidohydrolase which catalyzes the conversion of oxamate to oxalate and is a member of the ntn-hydrolase superfamily. hpxw is autoprocessed from an inactive precursor to form a heterodimer, resulting in a 35.5 kda α subunit and a 20 kda β subunit. here, the structure of hpxw is presented and the substrate complex ... | 2016 | 27303801 |
| association between the presence of aminoglycoside-modifying enzymes and in vitro activity of gentamicin, tobramycin, amikacin, and plazomicin against klebsiella pneumoniae carbapenemase- and extended-spectrum-β-lactamase-producing enterobacter species. | we compared the in vitro activities of gentamicin (gen), tobramycin (tob), amikacin (amk), and plazomicin (plz) against 13 enterobacter isolates possessing both klebsiella pneumoniae carbapenemase and extended-spectrum β-lactamase (kpc+/esbl+) with activity against 8 kpc+/esbl-, 6 kpc-/esbl+, and 38 kpc-/esbl- isolates. the rates of resistance to gen and tob were higher for kpc+/esbl+ (100% for both) than for kpc+/esbl- (25% and 38%, respectively), kpc-/esbl+ (50% and 17%, respectively), and kpc ... | 2016 | 27297487 |
| first report of extended-spectrum β-lactamases among clinical isolates of klebsiella pneumoniae in gaza strip, palestine. | sixteen broad-spectrum cephalosporin-resistant klebsiella pneumoniae isolates were recovered between april and june 2013 from palestinian hospitals, gaza strip. genes encoding extended-spectrum beta-lactamases (esbls) and other resistance genes were characterized by polymerase chain reaction and sequencing. the following β-lactamase genes were detected: blactx-m-15+ blashv1+ blatem-1 (six isolates), blactx-m-15+ blashv5+ blaoxa-1 (two isolates), blactx-m-14a (two isolates), blactx-m-15+ blashv33 ... | 2017 | 27294803 |
| rhinoscleroma with pharyngolaryngeal involvement caused by klebsiella ozaenae. | rhinoscleroma is a chronic, slowly progressive granulomatous bacterial infection that is endemic to the tropical world, namely, central america and africa. it is occasionally seen in the united states of america (usa). it predominately affects the nasal mucosa but can also involve the rest of the upper respiratory tract. the well-known causative agent for rhinoscleroma is the bacterium klebsiella rhinoscleromatis, a subspecies of klebsiella pneumoniae. however, klebsiella ozaenae can also, albei ... | 2016 | 27293924 |
| characterization of antimicrobial resistance in klebsiella species isolated from chicken broilers. | the prevalence of antimicrobial resistant klebsiella pneumoniae in poultry products has been a public concern, as it severely endangers food safety and human health. in this study, we investigated 90 antimicrobial resistant klebsiella strains that were isolated from a commercial broiler slaughter plant in shandong province of china. nearly all (89/90) of the isolates were identified as infectious phylogenetic group kpi-type k. pneumoniae. out of these 90 strains, 87 (96.7%) were multidrug-resist ... | 2016 | 27289192 |
| whole-genome comparative analysis of two carbapenem-resistant st-258 klebsiella pneumoniae strains isolated during a north-eastern ohio outbreak: differences within the high heterogeneity zones. | klebsiella pneumoniae has become one of the most dangerous causative agents of hospital infections due to the acquisition of resistance to carbapenems, one of the last resort families of antibiotics. resistance is usually mediated by carbapenemases coded for by different classes of genes. a prolonged outbreak of carbapenem-resistant k. pneumoniae infections has been recently described in northeastern ohio. most strains isolated from patients during this outbreak belong to mlst sequence type 258 ... | 2016 | 27289094 |
| performance of a pcr assay for the rapid identification of the klebsiella pneumoniae st258 epidemic clone in latin american clinical isolates. | the worldwide dissemination of klebsiella pneumoniae carbapenemase (kpc)-producing klebsiella pneumoniae st258 demands a rapid pcr-based typing method to detect unique genes of the st258 clone. this study evaluates a pcr developed by adler et al. (2014) for the detection of st258 in k. pneumoniae clinical isolates centered on the identification of the pilv-i and prp genes. we tested 143 clinical isolates from argentina (n=109), chile (n=1), colombia (n=1), costa rica (n=2), ecuador (n=5), el sal ... | 2016 | 27287559 |
| molecular evolution of a klebsiella pneumoniae st278 isolate harboring blandm-7 and involved in nosocomial transmission. | during 2013, st278 klebsiella pneumoniae with blandm-7 was isolated from the urine (kpn01) and rectum (kpn02) of a patient in calgary, canada. the same strain (kpn04) was subsequently isolated from another patient in the same unit. interestingly, a carbapenem-susceptible k. pneumoniae st278 (kpn06) was obtained 1 month later from the blood of the second patient. next-generation sequencing (ngs) revealed that the loss of carbapenem-resistance in kpn06 was due to a 5-kb deletion on the blandm-7-ha ... | 2016 | 27284091 |
| first evidence of polar flagella in klebsiella pneumoniae isolated from a patient with neonatal sepsis. | the genus klebsiella belongs to the family enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. in the present work, 25 klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. one klebsiella isolate, kpbuap021, demonstrated a swim-like motility phenotype. the k. pneumoniae genotype was confirmed by 16s rrna and rpob gene sequence analysis. multilocus sequence typing analysis also revealed that the kpbua ... | 2016 | 27283194 |
| modification and evaluation of the carba np test by use of paper strip for simple and rapid detection of carbapenemase-producing enterobacteriaceae. | carbapenemase-producing enterobacteriaceae (cpe) isolates have now emerged worldwide. we therefore modified the phenotypic carba np test by use of a filter paper strip for easily and rapidly identifying cpe in routine laboratory. a collection of 56 cpe and carbapenemase-producing pseudomonas spp. isolates (including 28 ndm-1, 11 imp-14a, 1 imp-1, 1 imp-4, 1 imp-9, 1 imp-15, 4 vim-2, 1 vim-1, 1 imp-14a with vim-2, 3 oxa-48, 3 oxa-181 and 1 kpc-2 producers) and 41 non-cpe isolates (including 19 es ... | 2016 | 27263012 |
| klebsiella pneumoniae primary liver abscess associated with ruxolitinib. | | 2016 | 27259987 |
| comparative activity of ciprofloxacin, levofloxacin and moxifloxacin against klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia assessed by minimum inhibitory concentrations and time-kill studies. | the aim of this study was to compare the in vitro susceptibility of klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia to three fluoroquinolones. the minimum inhibitory concentrations (mics) to ciprofloxacin, levofloxacin and moxifloxacin were examined by e-test® for a total of 40 k. pneumoniae strains, 40 s. maltophilia strains and 40 p. aeruginosa strains. then, the bactericidal activity of these fluoroquinolones was investigated on five strains of each bacterial sp ... | 2016 | 27257956 |
| defects in early cell recruitment contribute to the increased susceptibility to respiratory klebsiella pneumoniae infection in diabetic mice. | diabetes is associated with increased susceptibility to klebsiella pneumoniae and poor prognosis with infection. we demonstrate accelerated mortality in mice with streptozotocin-induced diabetes following tracheal instillation of k. pneumoniae. diabetic mice recruited fewer granulocytes to the alveolar airspace and had reduced early production of cxcl1, cxcl2, il-1β and tnf-α following tracheal instillation of k. pneumoniae-lipopolysaccharide. additionally, tlr2 and tirap expression following k. ... | 2016 | 27256462 |
| a combined disc method with resazurin agar plate assay for early phenotypic screening of kpc, mbl and oxa-48 carbapenemases among enterobacteriaceae. | to validate a combined disc method along with resazurin chromogenic agar for early screening and differentiation of klebsiella pneumoniae carbapenemase, metallo-β-lactamase and oxa-48 carbapenemase-producing enterobacteriaceae. | 2016 | 27253907 |
| structural and functional characterization of aerobactin synthetase iuca from a hypervirulent pathotype of klebsiella pneumoniae. | iron is a vital mineral nutrient required by virtually all life forms to prosper; pathogenic bacteria are no exception. despite the abundance of iron within the human host, highly regulated iron physiology can result in exceedingly low levels of iron bioavailable to prospective invading bacteria. to combat this scarcity of iron, many pathogenic bacteria have acquired specific and efficient iron acquisition systems, which allow them to thrive in iron-deficient host environments. one of the more p ... | 2016 | 27253399 |
| molecular dissection of blakpc-2-bearing plasmids evolving in klebsiella pneumoniae isolated at one teaching hospital in shanghai, china. | the presence of carbapenemase gene blakpc-2 in a wide variety of plasmids, especially conjugative plasmids, is key to the rapid, worldwide spread of carbapenemase enzymes. thirty-eight, non-duplicated, carbapenem-resistant, clinical klebsiella pneumoniae isolates were collected, all carrying blakpc-2-bearing plasmids. relaxase analysis was used to classify these plasmids; 8 and 30 plasmids belonged to the mobp3 and mobf12 subfamilies, respectively. phylogenetic analysis revealed two genetic subc ... | 2016 | 27252157 |