functional t-cell subset defined by cluster formation with eb virus transformed b-cells. | this study compares functional properties of t-cells capable of forming clusters with eb virus transformed b lymphoblastoid cells (b-lcl) as accessory cells (a-cells). t-cell functional properties examined include t-cell activation, interleukin 2 (il-2) production and cell proliferation. in addition, functional properties were compared with the presence or absence of surface markers. t-cells were divided into those that formed clusters with the b-lcl (clustered t-cells) and those that failed to ... | 1990 | 2175689 |
[analysis of b-lymphotropic oncogenic herpesvirus of macaca arctoides]. | some physico-chemical and molecular-biological parameters of b-lymphotropic herpes virus of macaca arctoides (hvma) have been analyzed. the buoyant density of virions and nucleocapsids in a gradient of the percoll density is 1.10 and 1.14 g/ml, respectively, while that of dna in cscl gradient is 1.717 g/ml, which is typical for herpes viruses of primates. at the same time the homology of dna hvma with dna ebv and dna hvp is 30-45%. differences in restrictions sites between dna hvma, dna hvp and ... | 1990 | 2175699 |
detection of human papillomavirus type 16 dna in carcinomas of the palatine tonsil. | twenty eight tonsillar carcinomas of various histological types were investigated for the presence of epstein-barr virus (ebv), cytomegalovirus (cmv), and human papillomavirus (hpv) types 6, 11, and 16 by in situ hybridisation using highly stringent procedures. in six cases an autoradiographic signal was obtained in the tumour cell nuclei with the hpv type 16 specific probe. no signal was obtained with any of the other probes. immunohistochemical investigations with mouse monoclonal antibodies d ... | 1990 | 2175756 |
oral melanotic macules in patients infected with human immunodeficiency virus. | a group of 217 patients seropositive for human immunodeficiency virus (hiv) were studied for 2 years, during which time pigmented lesions of the oral mucosa developed in 14 (6.4%) of them. the lesions were well circumscribed in some cases and diffuse in others. in some patients the macules enlarged or recurred after surgical excision. in two patients the macules appeared during the administration of zidovudine. clinical and laboratory evidence of adrenal insufficiency was not detected in any of ... | 1990 | 2175872 |
the enhancer factor r of epstein-barr virus (ebv) is a sequence-specific dna binding protein. | in cells latently infected with ebv, the switch from latency to productive infection is linked to the expression of two ebv transcription factors called eb1 (or z) and r. eb1 is an upstream element factor which has partial homology to the ap1/atf family, whereas r is an enhancer factor. in the r-responsive enhancer of the replication origin only active during the ebv lytic cycle (oriiyt), r-responsive elements are located in a region of about 70 bp (rre-dr). here we show that r, produced either ... | 1990 | 2175879 |
replication of chromosomal and episomal dna in x-ray-damaged human cells: a cis- or trans-acting mechanism? | episomal plasmids and viruses in mammalian cells present small targets for x-ray-induced dna damage. at doses up to 100 gy, dna strand breaks or endonuclease iii-sensitive sites were not discernible in 10.3-kb epstein-barr virus-based plasmid dna or in 4.9-kb defective simian virus 40 dna. dna replication in these small molecules, however, was inhibited strongly by x-ray doses of greater than or equal to 20 gy, decreasing to only 20 to 40% of control values. inhibition was relieved slightly by g ... | 1990 | 2175922 |
[monoclonal anti-d antibodies: obtaining and use]. | the principles of obtaining monoclonal antibodies against d-antigen of the rhesus system are overviewed. the lines secreting anti-d antibodies are characterized. the strategies for selecting the anti-d producers secreting the antibodies are substantiated, which are useful for developing rhesus-typing diagnostics and preparations to prevent rh-sensitization. | 1990 | 2176019 |
aggressive hepatitis in a patient with acute myeloid leukaemia during complete remission and detection of epstein-barr virus dna in a liver biopsy. | | 1990 | 2176093 |
herpesvirus and enteric viral infections in bone marrow transplantation: clinical presentations, pathogenesis, and therapeutic strategies. | | 1990 | 2176125 |
[a case of facial diplegia due to eb virus infection]. | a case of facial diplegia due to eb virus infection is reported. a 56-year-old man developed headache, arthralgia and low grade fever. two days later he noted dysesthesia of the bilateral extremities. eight days later, disturbances of bilateral mouth and eye closing appeared, which brought him to our hospital on january 6, 1986. neurological examinations disclosed bilateral peripheral facial palsy and glove and stocking type sensory impairment. muscle weakness, pathological reflexes were not not ... | 1990 | 2176141 |
in vitro anticolon antibody production by mucosal or peripheral blood lymphocytes from patients with ulcerative colitis. | serum anticolon antibody and in vitro anti-colon antibody production by peripheral blood and mucosal lymphocytes was investigated in patients with ulcerative colitis. the frequency of serum anticolon antibody was 71% in 41 patients with ulcerative colitis, estimated by enzyme linked immunosorbent assay (elisa) using isolated rat colon epithelial cells. this finding confirms our previous report on the frequency of serum anticolon antibody detected by flow cytometry analysis. the estimated frequen ... | 1990 | 2176171 |
an enzyme-linked immunosorbent assay for igg antibodies to human herpes virus 6. | an indirect enzyme-linked immunosorbent assay (elisa) with human herpes virus 6 (hhv6) membrane antigen was compared with indirect immunofluorescence assay (ifa) for measurement of hhv6 igg antibodies. five hundred serum samples from 403 swedish patients with suspected symptomatic epstein-barr virus (ebv) infections were examined. the specificity of the elisa compared with ifa was 98.7% and the sensitivity was 98.4%. in 90% of the patients, igg antibodies to hhv6 were detected with both assays. ... | 1990 | 2176224 |
oral hairy leukoplakia: a histopathologic study of 32 cases. | oral hairy leukoplakia (hl) was studied in 32 patients infected with the human immunodeficiency virus (hiv). features most often found were parakeratosis of variable thickness, acanthosis, little or absent submucosal chronic inflammatory cell infiltrate, association with candidiasis in 71.8%, and intranuclear herpetic type inclusions in keratinocytes, associated with ballooned or ground-glass cytoplasm. the last feature was found in all biopsies and was most striking at the top of the stratum sp ... | 1990 | 2176438 |
molecular characterization of a cdna clone encoding the epstein-barr virus (ebv) dnase. | rna from idurd-treated p3hr1 cells was used for the construction of a cdna library and screened with b95-8 ebv dna bamhi fragment b and g probes. one clone, bg9, containing a 1.7 kb cdna insert was further studied. complete dna sequence analysis revealed that bg9 encompassed the b95-8 ebv dna sequences from nucleotide 120,747 to nucleotide 122,412 and corresponded to the bglf5 open reading frame of the ebv dnase gene. comparison of the sequences of bg9 with that of published b95-8 ebv dna indica ... | 1990 | 2176660 |
oxidative burst in human epstein-barr virus-transformed b-cell lines triggered by immobilized specific antigen. | normal human b lymphocytes and epstein-barr virus-transformed b-cell lines can produce reactive oxygen species such as superoxide if treated with phorbol myristate acetate (pma) or with the surface immunoglobulin cross-linking agents protein a and anti-immunoglobulin. here, we investigated under which conditions specific antigen, the natural ligand of surface immunoglobulin, can stimulate an oxidative burst in monoclonal epstein-barr virus-transformed b-cell lines producing antibodies of known s ... | 1990 | 2176740 |
roseola infantum and other syndromes associated with acute hhv6 infection. | eight cases of acute human herpesvirus type 6 (hhv6) infection in infants were diagnosed serologically by the demonstration of igm anti-hhv6 (8/8) and a significant change in total anti-hhv6 antibody titre (6/8). four infants were sufficiently ill to require admission to hospital and further investigations: one with encephalitis and three with gross hepatosplenomegaly, two of whom had evidence of simultaneous infection with another herpes-virus. the remaining four infants had an illness compatib ... | 1990 | 2176778 |
molecular resemblance of an aids-associated lymphoma and endemic burkitt lymphomas: implications for their pathogenesis. | | 1990 | 2176940 |
[gene engineering eb virus membrane antigen in detection of ma-iga antibody--comparison with vca-iga and ea-iga antibodies]. | with gene engineering eb virus membrane antigen as the diagnostic antigen, indirect immunofluorescence (if) assay was used to detect iga antibody against eb virus membrane antigen (ma-iga) in sera from 202 nasopharyngeal carcinoma (npc) patients and 315 controls (normal and patients with other tumors). ma-iga antibody was positive in 96.8% of the pre-treatment npc patients with a gmt of 1:36.3. ma-iga detection by this method was more sensitive than ea-iga detection by ie. in contrast, patients ... | 1990 | 2176967 |
immune deficiency as a risk factor in epstein-barr virus-induced malignant diseases. | epstein-barr virus (ebv) is a ubiquitous dna virus that normally infects silently, establishing lifelong latency. substantial empirical observations support the view that immunodeficiency is permissive in ebv-induced lymphoproliferative diseases (lpd). primary immune deficient patients such as those with x-linked lymphoproliferative disease and individuals with acquired immune deficiency secondary to immunosuppressive drugs for organ transplantation or individuals infected with human immunodefic ... | 1990 | 2176975 |
induction and progression of human lymphoproliferative lesions by epstein-barr virus. | epstein-barr virus (ebv) is involved in numerous lymphoproliferative diseases. in addition to classical lesions such as endemic burkitt's lymphoma and infectious mononucleosis, there are other disorders of the lymphoid system that are discussed in relation to ebv: b-cell lymphomas in immunosuppressed individuals. hodgkin's disease and, to some extent, primary extranodal lymphomas. studies of the ebv expression in classical and nonclassical lesions could lead to the better understanding of differ ... | 1990 | 2176977 |
multiple small bowel perforations in leukemia secondary to epstein-barr virus lymphoma with survival: a case report. | this is the case report of a 4-year-old white boy who was diagnosed as having acute lymphoblastic leukemia (all) in november 1985. while in remission and on maintenance chemotherapy, he developed a primary epstein-barr virus (ebv) respiratory infection in october 1986. on october 27, 1986 a plain abdominal radiograph taken for abdominal distention showed free air. at celiotomy, multiple nodules were noted to stud the small bowel. central necrosis of these nodules with perforations were present i ... | 1990 | 2177105 |
antibodies to synthetic peptides from epstein-barr nuclear antigen-1 in sera of patients with early rheumatoid arthritis and in preillness sera. | we studied igg antibody levels to synthetic peptides (p62, e11 and e3) from epstein-barr nuclear antigen-1 (ebna-1) protein sequence in sera of patients with rheumatoid arthritis (ra), in pre-ra and in rheumatoid factor (rf) positive non-ra sera from finland. anti-e11 and anti-e3 antibody levels were significantly elevated in ra sera, compared with both rf negative and rf positive nonrheumatoid controls. the concentrations of anti-e11 and anti-e3 antibodies in the preillness sera were lower than ... | 1990 | 2177109 |
role of atl-derived factor (adf) in the normal and abnormal cellular activation: involvement of dithiol related reduction. | htlv-i transformed t cells not only express a large number of interleukin-2 receptors (il-2r/p55(tac], but also produce an il-2r/tac inducer named atl-derived factor (adf). we have cloned the adf cdna and found that adf production in human lymphocytes can be enhanced by cellular activators such as mitogens or phorbol esters. recombinant adf produced by e. coli was shown to have growth-promoting activity in combination with interleukin-2 or suboptimal mitogenic stimuli on several lymphoid cells i ... | 1990 | 2177148 |
c3d-mediated negative and positive signals on the proliferation of human b cells separated from blood. | soluble c3d applied to human blood-derived b lymphocytes inhibited anti-mu, t cell-produced growth factor, and ebv-induced dna synthesis in serum-free culture. c3d added to the b cell cultures 1 and 2 days after the stimulus, still exerted inhibition, though with gradually diminishing efficiency. c3d, fixed on zymosan or attached to the culture wells, induced [3h]thymidine incorporation of the b cells in serum-free medium. the concentration of c3d used to coat the wells was critical, with optima ... | 1990 | 2177450 |
epstein-barr virus-carrying b cells in the blood during acute infectious mononucleosis give rise to lymphoblastoid lines in vitro by release of transforming virus and by proliferation. | in accordance with earlier studies, we detected higher numbers of epstein-barr virus (ebv)-carrying lymphocytes (b-ebv) in the blood of acute infectious mononucleosis (im) patients and higher amounts of transforming ebv particles in the saliva compared to healthy seropositive individuals. b cells grew in cultures seeded with the low and high density im lymphocytes. the majority of b cells which grew acquired the infection in vitro (2-step outgrowth), because addition of virus neutralizing antibo ... | 1990 | 2177451 |
non-tumorigenicity of bloom's syndrome lymphoblastoid cell lines in immunodeficient mice. | | 1990 | 2177613 |
ebv utilizes a unique activation pathway for the transformation of human b cells. | ebv growth-transforms primate b lymphocytes and directly causes mono/multiclonal b cell lymphomas in vulnerable hosts. in this report we demonstrate that the degree of b cell transformability is not quantitatively determined at the level of either the saturable, transformation-prerequisite virus receptors or of the actual viral cell entry process. instead, post-receptor binding events [na+/h+ exchange, ca2+ flux, tyrosine phosphorylation of two proteins (55-60/130-140 kd)] were identified as cri ... | 1990 | 2177646 |
diagnosis of epstein-barr virus infection in hairy leukoplakia by using nucleic acid hybridization and noninvasive techniques. | the presence of epstein-barr virus (ebv) dna in the epithelial cells of oral hairy leukoplakia is the confirming criterion in the diagnosis of this lesion, which occurs mainly in persons infected by the human immunodeficiency virus. because hairy leukoplakia often presages the development of the acquired immune deficiency syndrome, it is important that suspicious lesions be accurately diagnosed. commonly, biopsy tissue is removed for detection of ebv dna by in situ hybridization, but biopsy is c ... | 1990 | 2177752 |
oligonucleotides for polymerase chain reaction amplification and hybridization detection of epstein-barr virus dna in clinical specimens. | we designed synthetic oligonucleotide primers and hybridization probe for use in polymerase chain reaction (pcr) amplification and hybridization detection of epstein-barr virus (ebv) nucleic acid sequences. primer sequences were chosen from the coding region for the epstein-barr virus nuclear antigen-1 (ebna-1). pcr amplification and hybridization with these oligonucleotides was carried out on standard laboratory cell lines including african burkitt's lymphoma and infectious mononucleosis derive ... | 1990 | 2177846 |
viral oncogenesis: epstein-barr virus. | the epstein-barr virus (ebv) is a common herpesvirus that has been linked to several human neoplasms. it has been found in over 90% of cases of burkitt's lymphoma in endemic regions, although only 15% of cases in nonendemic regions show evidence of infection. the virus has been found in malignant lymphomas in immunocompromised states, including the acquired immunodeficiency syndrome, iatrogenic immunosuppression, such as in posttransplantation patients, and congenital immunodeficiencies. recentl ... | 1990 | 2177957 |
methyl transferase activity in secondary leukemia. | | 1990 | 2177980 |
medical eponyms updated: 3. burkitt's lymphoma. | | 1990 | 2177998 |
rapid expansion of human immunoglobulin repertoire (vh, v kappa, v lambda) expressed in early fetal bone marrow. | we have isolated pre-b- and b-cell clones after transformation by epstein-barr virus (ebv) of human fetal bone marrow cells between weeks 8 and 13 of gestation. these clones were characterized for immunoglobulin (ig) chain synthesis, the status (rearranged versus germ line) of the heavy (h) chain, kappa, and lambda loci, and of the ig mrna transcripts by using specific variable (v), vh, v kappa, and v lambda probes covering the almost complete igv repertoire. mature b cells could already be iden ... | 1990 | 2178002 |
susceptibility to hiv-1 infection of a human b-lymphoblastoid cell line, dg75, transfected with subgenomic dna fragments of epstein-barr virus. | we have previously shown that the presence of the ebv genome and active ebv infection in some burkitt's lymphoma cell lines confer the susceptibility of hiv-1 infection in spite of the absence of surface cd4 receptors in these cells. in the present study we used an ebv genome negative burkitt's lymphoma b-cell line, dg75, transfected with three different subgenomic fragments of ebv expressing the nuclear antigens (ebna1 and ebna2) and the latent membrane protein (lmp), respectively. immunofluore ... | 1990 | 2178126 |
isolation of human herpesvirus-6 from clinical specimens using human fibroblast cultures. | the isolation and characterization of human herpesvirus-6 (hhv-6) has been hindered by the lack of cell lines useful for its rapid propagation. recently, we have reported that the mrc-5 cell line (human diploid lung fibroblasts) was susceptible for hhv-6 infection. in this study, we report on the isolation of hhv-6 from the peripheral blood or buffy coat of three chronic fatigue syndrome patients, one post-liver transplant patient, and one severe chronic active epstein-barr virus syndrome patien ... | 1990 | 2178187 |
undifferentiated carcinoma with lymphoid stroma of the major salivary glands. | undifferentiated carcinoma with lymphoid stroma or lymphoepithelial carcinoma of the major salivary glands is a demographically and histopathologically unique malignancy. although whites may have the disease, it is preponderantly a carcinoma of north american eskimos and native greenlanders. the carcinoma shares many features with undifferentiated nasopharyngeal carcinomas, from which it must be distinguished: histologic appearance, putative relationship with epstein-barr virus, predilection for ... | 1990 | 2178544 |
endothelial cells modulate both t-cell-dependent and t-cell-independent plaque-forming cell generation in vitro. | the effects of live endothelial cells (ec), paraformaldehyde fixed ec, and ec supernatant were measured on pokeweed mitogen (pwm)-induced t-cell-dependent plaque-forming cell (pfc) generation by peripheral blood mononuclear cell (pbm). at low doses (less than or equal to 2 x 10(4) cells/culture) live ec helped pfc generation. at higher doses (greater than or equal to 10 x 10(4) cells/culture) the effect of live ec was always marked suppression (less than 10% of baseline pfc). in contrast both fi ... | 1990 | 2179140 |
thymic carcinoma. report of five cases and review of the literature. | among 54 mediastinal tumours we examined in the past 20 years, there were 5 cases of primary thymic carcinomas, each with widespread metastases. histological features in three cases were consistent with lymphoepithelioma-like carcinoma. one case showed an epidermoid pattern with keratotic pearls resembling hassall bodies. one undifferentiated carcinoma developed from a cortical thymoma. epstein-barr virus could not be detected in tumour tissue with in situ hybridization. a review of the literatu ... | 1990 | 2179229 |
aids-associated lymphoma. | like other immunodeficient populations, hiv-infected individuals are at risk for developing high grade b-cell malignancies. the aetiology of these lymphomas remains unknown. while the tumours share many of the features of b-cell lymphomas seen in immunosuppressed transplant recipients, unlike transplant recipients, epstein-barr virus genomic sequences are identified in only a small minority of peripheral lymphomas from hiv-infected individuals. the majority of lymphomas are classified as diffuse ... | 1990 | 2182138 |
infection after liver transplantation. | infections occurring in liver transplant recipients result in significant morbidity and mortality. factors influencing the frequency of posttransplant infections include pretransplant nutritional status, latent viral infections, and the degree of immunosuppression used to modulate the immune response to the allograft. infectious agents may be introduced into the patient via the allograft, through infusion of blood products, and through intravenous lines, catheters, and drains. infections also de ... | 1990 | 2183644 |
neurasthenia in the 1980s: chronic mononucleosis, chronic fatigue syndrome, and anxiety and depressive disorders. | in the 1980s, patients suffering from unexplained fatigue and what seemed like a prolonged attack of acute mononucleosis were given the diagnosis of chronic mononucleosis or chronic infection with the epstein-barr virus. although the diagnosis has great appeal, the epstein-barr virus does not cause the syndrome (cfs) of chronic fatigue, which has been renamed and redefined chronic fatigue syndrome to remove the inference that the virus is its cause. from a historical perspective, both syndromes ... | 1990 | 2184452 |
malignant lymphomas associated with immunodeficiency states. | an increased incidence of malignant lymphomas is common to all types of immunodeficient patients whether they be of the natural or constitutionally occurring type, acquired as in acquired immunodeficiency syndrome (aids) or of iatrogenic origin as in organ transplantation. although there is some degree of heterogeneity, the most characteristic feature of these immunodeficient states is alteration of t-cell cytotoxic function. the malignant lymphomas show a variety of relatively common features, ... | 1990 | 2188564 |
aetiology of nasopharyngeal carcinoma. | nasopharyngeal carcinoma (npc), a rare tumour in most parts of the world, occurs in high frequencies in china and south east asia and is particularly high among the southern chinese. in certain parts of china the incidence may reach 50 per 10(5) per year while in most other ethnic groups the incidence is very low, e.g. among caucasians the incidence is less than 1 per 10(5) per year. these marked geographic and racial differences in incidence have stimulated much research into the aetiology of t ... | 1990 | 2189340 |
construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents. | prior to constructing a library of yeast artificial chromosomes (yacs) containing very large human dna fragments, we performed a series of preliminary experiments aimed at developing a suitable protocol. we found an inverse relationship between yac insert size and transformation efficiency. evidence of occasional rearrangement within yac inserts was found resulting in clonally stable internal deletions or clonally unstable size variations. a protocol was developed for preparative electrophoretic ... | 1990 | 2190217 |
the extrachromosomal replication of dictyostelium plasmid ddp2 requires a cis-acting element and a plasmid-encoded trans-acting factor. | dictyostelium discoideum plasmid ddp2 from the wild strain ws380b is a 5.8-kilobase (kb) supercoiled circle with a copy number of 300 per haploid genome. we previously described the construction of an extrachromosomally replicating transformation vector pndei carrying 4.7 kb of ddp2 sequences (b. leiting, and a. noegel, plasmid 20:241-248, 1988). in order to reduce the sequences required for extrachromosomal maintenance in d. discoideum, we characterized ddp2 by sequence analysis, by deletion ex ... | 1990 | 2192261 |
serum igg and iga antibodies specific to epstein-barr virus capsid antigen in a longitudinal study of human immunodeficiency virus infection and disease progression in homosexual men. | a longitudinal study of serum igg and iga antibody titers to epstein-barr virus (ebv) viral capsid antigen (vca) was carried out in 218 homosexual men at various stages of human immunodeficiency virus (hiv) infection. the serum samples tested were obtained from the following groups: 24 hiv seroconverters, 41 persistently hiv-seropositive asymptomatic individuals, 22 seropositives who developed aids-related complex (arc), 29 hiv seropositives who developed lymphadenopathy syndrome (las), 35 hiv s ... | 1990 | 2193673 |
lymphoma and other hiv-associated malignancies. | the immunodeficient state that evolves in persons infected with the human immunodeficiency virus (hiv) appears to increase their risk of certain types of cancer. among these are primary lymphoma of the central nervous system, undifferentiated non-hodgkin's lymphoma, squamous cell carcinoma, anorectal carcinoma, and cutaneous malignancies. these malignancies are similar in incidence to those seen in other immunodeficient patients. lymphoma, in particular, is associated with a more aggressive dise ... | 1990 | 2193954 |
t-cell-depleted autologous bone marrow transplantation therapy: analysis of immune deficiency and late complications. | fourteen patients with t-cell-derived leukemia and lymphoma underwent high-dose chemoradiotherapy and anti-t-cell monoclonal antibody-treated autologous bone marrow transplantation (abmt). all patients were either in sensitive relapse or had adverse prognostic features, and five patients had a history of bone marrow involvement with disease. patients received a median of 2 (1 to 3) prior chemotherapy regimens; 10 patients received local radiotherapy. after high-dose ablative therapy, greater tha ... | 1990 | 2194591 |
binding activity and autophosphorylation of the insulin receptor from patients with myotonic dystrophy. | to clarify a mechanism of insulin resistance associated with myotonic dystrophy, we studied the insulin receptor by using three different types of cells--circulating erythrocytes, cultured skin fibroblasts, and epstein-barr virus(ebv)-transformed lymphocytes. in myotonic dystrophy, insulin binding to erythrocytes and fibroblasts was significantly decreased as a result of a reduction of the binding affinity. insulin binding to ebv-transformed lymphocytes was normal. when the receptors were purifi ... | 1990 | 2195130 |
virus infections in patients with aids. | virus infections are common in patients with acquired immune deficiency syndrome (aids). viruses can have two distinct relationships with human immunodeficiency virus (hiv); they can be opportunists if the second virus takes advantage of decreased immune function in the host or they can act as co-factors to accelerate the rate at which aids develops. viruses acting as opportunists may cause no symptoms or may be life-threatening. several, including herpes simplex, varicella zoster, cytomegalovir ... | 1990 | 2201112 |
enumeration of ige secreting b cells. a filter spot-elisa. | to assess the frequency of ige producing cells in humans a filter immunoplaque assay has been developed to detect ige secretion from individual b lymphocytes in unfractionated peripheral blood mononuclear cells (pbmc). pbmc were incubated in microfilter plates containing nitrocellulose membranes coated with polyclonal anti-human ige antibody, and the ige production by a single cell was detected using a specific anti-human ige monoclonal antibody followed by enzymatic development. the products of ... | 1990 | 2202765 |
[oral hairy leukoplakia in patients with hiv infection]. | oral hairy leukoplakia is a new clinicopathological entity. little is known about its etiology, pathogenesis and natural history. it is considered a specific marker of hiv infection, although it has been also reported in hiv-negative patients. the etiology appears related to epstein-barr virus. hairy leukoplakia has a characteristic clinical and histopathological aspect, but it is not specific. because hairy leukoplakia may be easily confused with other lesions of the tongue, the dentist should ... | 1990 | 2204797 |
iscoms. | the common problem for defined purified antigens or antigen determinants has been to make them immunogenic regardless of whether they are produced conventionally, produced as cloned genetechnology products or chemically synthesized. the first problem is to get the protective antigen into a submicroscopic particle where the antigen is presented in several copies, i.e. as a multimer. for some antigens it seems also necessary to enhance the immunogenicity with an adjuvant. the immunostimulating com ... | 1990 | 2205974 |
agranulocytosis following infectious mononucleosis. | a girl developed acute agranulocytosis (45/mm3), 37 days after the onset of infectious mononucleosis. the bone marrow showed myeloid hyperplasia with maturation arrest and erythroid hypoplasia. a normal amount of colony forming units of granulocytes and macrophages (cfu-gm) colonies with a relative high number of clusters was observed. neither anti-neutrophil antibodies nor circulating inhibitors of colony growth were found in serum. granulocyte and macrophage colony stimulating factor (gm-csf) ... | 1990 | 2209660 |
autoantibody production by severe combined immunodeficient mice reconstituted with synovial cells from rheumatoid arthritis patients. | in an attempt to characterize the heterogeneity of the human autoantibody response, mice with severe combined immunodeficiency were reconstituted with synovial or blood lymphocytes from patients with rheumatoid arthritis (ra). mononuclear cells extracted from synovial fluid or tissue (smc) were a greatly enriched source of igm rheumatoid factor (rf)-producing cells compared to the peripheral blood mononuclear cells (pbmc) of rheumatoid arthritis patients or normal donors. six to nine weeks after ... | 1990 | 2209691 |
antigens recognized on a melanoma cell line by autologous cytolytic t lymphocytes are also expressed on freshly collected tumor cells. | peripheral blood lymphocytes of a melanoma patient were stimulated in vitro with a permanent cell line derived from the autologous tumor. stable cytolytic t lymphocyte (ctl) clones were obtained that lysed the melanoma cell line and did not lyse autologous epstein-barr virus-transformed b lymphocytes or k-562 cells. these ctl clones were directed against two distinct antigens on the melanoma line. in view of the possibility that these antigens could be culture artefacts, we tested the stimulator ... | 1990 | 2209693 |
t cell target 1 (tct.1): a novel target molecule for human non-major histocompatibility complex-restricted t lymphocytes. | we have studied two gamma/delta t cell clones, e102 and e117, generated in a mixed lymphocyte culture using an allogeneic epstein-barr virus-transformed b cell line, e418. these clones were both found to express a molecular form of t cell receptor (tcr) infrequent in human peripheral blood, associating a v1-j1-c delta chain and a v3-jp2-c2 gamma chain. functionally, they appeared as cytotoxic t lymphocytes (ctl) with non-major histocompatibility complex (mhc) (class i and ii) requiring cytotoxic ... | 1990 | 2212943 |
toxoplasmic chorioretinitis and hepatic granulomas. | a 71-yr-old male presented with a 2-month history of fever, malaise, and weight loss. physical exam revealed chorioretinitis. laboratory studies were notable for elevated levels of alkaline phosphatase, gamma-glutamyl transpeptidase, aspartate transaminase, and alanine transaminase. immunoglobulin g antibody to toxoplasma gondii was positive to a dilution of 1:4096, whereas serologic studies for hepatitis a virus, hepatitis b virus, cytomegalovirus, epstein-barr virus, human immunodeficiency vir ... | 1990 | 2220741 |
acute sore throat. | the second common problem to be presented in this series is the acute sore throat. the common causes are viral pharyngitis and tonsillitis due to streptococcus pyogenes. another important cause that warrants attention is epstein barr virus (infectious mononucleosis) so that prescribing of penicillins is carefully considered. the sore throat may be the presentation of serious and hidden systemic diseases, such as blood dyscrasias, aids and diabetes (due to moniliasis). | 1990 | 2222302 |
viral oncogenesis and the immune system. | oncogenic transformation of normal cells and the establishment of transformed cells to form malignant tumors is a complex, multistep process influenced by viruses in multiple ways. the relationship between viruses and the immune system manifests itself, in part, through various roles of viruses in transformation of host cells, including cells of the immune system. a large number of viruses participate in oncogenic transformation of cells in many animal species. candidates for oncogenic transform ... | 1990 | 2224909 |
activity of glucocerebrosidase in extracts of different cell types from type 1 gaucher disease patients. | glucocerebrosidase activity in extracts of leukocytes, epstein-barr virus transformed lymphocytes and fibroblasts from portuguese type 1 gaucher disease patients was studied. the residual glucocerebrosidase activity in all extracts from patients was less than 25% if measured in the presence of bile salt taurocholate. however, if measured in the absence of bile salt the residual enzyme activity in extracts from patients was cell type specific: it was severely reduced in the case of fibroblasts, m ... | 1990 | 2225530 |
[acquired immunodeficiency]. | secondary immunodeficiencies are much more frequent than primary immunological disorders. there are many etiologies, such as malnutrition, cytostatic drug therapy, burns, protein-losing conditions and infections. this review briefly considers the history of virus-induced immunosuppression. today, measles and aids are the most important virus-induced immunodeficiency diseases. in rate instances, infections with epstein-barr virus can lead to hypogammaglobulinemia and b cell lymphomas. a viral eti ... | 1990 | 2227749 |
unusual lymphoproliferative oropharyngeal lesions in heart and heart-lung transplant recipients. | three unusual cases of oropharyngeal lympho-proliferative lesions were seen in recipients of heart and heart-lung transplants. two caused acute upper respiratory obstruction necessitating urgent ent intervention. all patients were receiving immunosuppressive drugs including cyclosporin. the two obstructive cases were adenotonsillar enlargement in a 6-year-old, and a tumour of the tonsil and tongue base with cervical lymph node enlargement in a 32-year-old male. both were caused by epstein-barr v ... | 1990 | 2230584 |
the control of o6-methylguanine-dna methyltransferase (mgmt) activity in mammalian cells: a pre-molecular view. | human peripheral blood lymphocytes (pbls) can have a range of o6-methylguanine-dna methyltransferase (mgmt) activities. pbls from some individuals may have almost no mgmt activity. such individuals have most often been subject to malignancy or to immunodeficiency disease. long-term lymphoblastoid lines (lcls) prepared from pbls of normal subjects by epstein-barr virus (ebv) transformation have mgmt activities which are in general somewhat higher than the pbls from which they derive. such culture ... | 1990 | 2233795 |
down-regulation of lfa-1 adhesion receptors by c-myc oncogene in human b lymphoblastoid cells. | the function of the c-myc gene and its role in tumorigenesis are poorly understood. in order to elucidate the role of c-myc oncogene activation in b cell malignancy, the phenotypic changes caused by the expression of c-myc oncogenes in human b lymphoblastoid cells immortalized by epstein-barr virus were analyzed. c-myc oncogenes caused the down-regulation of lymphocyte function-associated antigen-1 (lfa-1) adhesion molecules (alpha l/beta 2 integrin) and loss of homotypic b cell adhesion in vitr ... | 1990 | 2237417 |
the internist in the management of head and neck cancer. | the general internist has an important role in the management of head and neck squamous cell cancers. this heterogeneous group of cancers must be accurately diagnosed and staged before planning treatment. curability is directly related to stage at presentation and, because most patients with such cancers present to internists first, these physicians must be familiar with presenting symptoms and must be suspicious enough to refer patients with symptoms for appropriate evaluation. the work-up of p ... | 1990 | 2240879 |
infectious mononucleosis in older adults. | infectious mononucleosis as a manifestation of primary epstein-barr virus infection occurs uncommonly in adults over age 40. while fever is almost universal, older patients with the disease often present without lymphadenopathy, pharyngitis, splenomegaly, lymphocytosis or atypical lymphocytes. jaundice and hepatomegaly occur more commonly in older patients than in adolescents and create diagnostic confusion. often, infectious mononucleosis in this age group is confused with lymphoma, leukemia or ... | 1990 | 2244552 |
angiocentric immunoproliferative lesion/t-cell non-hodgkin's lymphoma and the acquired immune deficiency syndrome: a case report and review of the literature. | the lesions known as lymphocytic vasculitis, polymorphic reticulosis (midline malignant reticulosis, lethal midline granuloma), lymphomatoid granulomatosis, and angiocentric lymphoma form what have been collectively termed the angiocentric immunoproliferative lesions (ail). because of recent reports demonstrating clonal rearrangements of the t-cell receptor in these lesions, the ail are now thought to represent a continuous spectrum of post-thymic t-cell non-hodgkin's lymphoma (nhl). nhl associa ... | 1990 | 2245397 |
establishment and characterization of a new human b-cell line (onhl-1) from non-hodgkin's lymphoma: constant expression of bcl-2 gene during mitogen-induced growth inhibition. | a new b-cell line (onhl-1) was established from non-hodgkin's lymphoma. onhl-1 was free from epstein-barr virus nuclear antigen and expressed cd20, cd24, and slg (mu, delta, gamma and kappa), thus being equivalent to the mature b-cell stage. chromosome analysis revealed a markedly abnormal pattern including 14q+ and 6q-. in accordance with the positive expression of surface kappa light chains, one of the kappa genes was found to be rearranged. however, rearrangement of the lambda locus was also ... | 1990 | 2249898 |
biological characterization of recombinant vaccinia viruses in mice infected by the respiratory route. | a murine model based on infection by the respiratory route has been used to study the pathogenesis of recombinant vaccinia viruses. the neurovirulent western reserve (wr) strain and the wyeth smallpox vaccine strain were used as vectors. recombinant viruses were constructed by insertion of the epstein-barr virus membrane glycoprotein 340 gene into the thymidine kinase (tk) gene of each vaccinia virus. intranasal inoculation of dba/2 mice with 10(6) pock-forming units (pk.f.u.) of the wr strain w ... | 1990 | 2254756 |
[why are there anti-cardiolipin antibodies in patients with infectious mononucleosis and in patients with systemic lupus erythematosus?]. | the phenomenon of anticardiolipin antibodies (acl) is in recent years the subject of interest as it is connected with manifestations of thromboses at different sites, with repeated miscarriages in pregnant women and with thrombocytopenia. as compared with the original finding in patients with systemic lupus erythematosus (sle), the authors find they are present also in infection with the epstein-barr virus--in patients with acute infectious mononucleosis, in as many as 61% of a total number of 7 ... | 1990 | 2267733 |
structure and expression of the human polymorphic epithelial mucin gene: an expressed vntr unit. | the human polymorphic epithelial mucin (pem) is expressed apically by glandular epithelium and by the carcinomas that develop from these tissues. previously isolated cdna clones revealed that the core protein contained a large domain consisting of variable numbers of 60 bp tandem repeats (tr), making it an expressed minisatellite. we now report the full genomic sequence of the pem gene, including 803 bp of 5' flanking sequence. the gene is composed of 7 exons and varies in size from approximatel ... | 1990 | 2268309 |
[the viral and immunological characteristics of cardiomyopathies and myocarditis]. | as many as 97 patients with myocardial lesions: congestive and hypertrophic cardiomyopathy (cmp), postmyocarditis cmp (pm cmp), myocarditis (mc), alcoholic heart injury (ahi), coronary heart disease (chd), vegetodysovarian myocardiodystrophy were examined by means of a complex of the virological tests (for coxsackie b, epstein-barr and hepatitis b viruses) and immunoassays (for antibodies to different components of the myocardium, leukocyte migration inhibition test, antibody-dependent cellular ... | 1990 | 2274878 |
[regression of cerebral post-transplantation lymphoma under cyclosporin a reduction]. | 4.5 months after successful kidney transplantation a non-hodgkin-lymphoma with polymorph centroblastic appearance of the tonsillar gland developed in a 21 years old male patient during immunosuppressive therapy with cyclosporine a and prednisone parallel to infection with epstein barr virus. focal epileptic seizures occurred and were due to cerebral posttransplantation lymphomas as proven by brain biopsy. reduction of immunosuppressive therapy led to complete remission as shown by cct and mri. | 1990 | 2280583 |
the iscom: an immunostimulating system. | to make purified antigens highly immunogenic, they have to be presented in several copies in the form of a microscopic or submicroscopic particle. this is the case, regardless of whether the antigens are obtained by isolation from conventional microorganisms, or from gene-manipulated cells, or synthesized. in the iscom, the antigens are attached as multimers to a 40-nm cage-like particle with a built-in adjuvant. the antigens in iscoms are rapidly transported from the injection site to the drain ... | 1990 | 2283159 |
[oropharyngeal and rhinopharyngeal carcinomas in patients treated for non-hodgkin's lymphoma]. | the authors describe two cases of nasopharynx and oropharynx carcinomas in treated non-hodgkin lymphoma's patients. they evaluate pathogenetic hypotheses related to lymphoma, its treatment and some exogenous factors like smoke, alcohol, epstein-barr virus. | 1990 | 2291014 |
burkitt's lymphoma in ethiopian children. | burkitt's lymphoma is the commonest malignancy seen in childhood in ethiopia. thirty-nine children admitted to the ethio-swedish children's hospital from january 1971 to december 1988 were studied. the male to female ratio was 1.3:1, 24(63%) were from non-malarious areas, 29(76%) came from areas with altitude above 1500 meters above sea level, 36(95%) came from areas with mean annual temperature above 16 degrees c and 35(61%) patients came from areas with mean annual rainfall above 1000 mm. the ... | 1990 | 2293434 |
selective expression of the p70 subunit of the interleukin-2 receptor on lymphocytes from patients with infectious mononucleosis. | the lymphocytosis manifested in infectious mononucleosis (im) during acute phase is ascribed to a reactive expansion of cd8+ t lymphocytes caused by epstein-barr virus (ebv)-infected b lymphocytes. expression of hla-dr antigen on im lymphocytes suggests that these t lymphocytes are somehow activated in vivo. in the present study, we analyzed the interleukin-2 (il-2) receptor expression on lymphocytes from six patients with acute im. radiolabeled il-2 binding assay revealed that im lymphocytes fr ... | 1990 | 2295000 |
sinus histiocytosis with massive lymphadenopathy and malignant lymphoma. an unreported association. | sinus histiocytosis with massive lymphadenopathy is a benign disorder often characterized by spontaneous remission. although immunologic abnormalities are common and high epstein barr virus titers have been reported, to date no case has been associated with malignant lymphoma. the authors report here the first case of this association. | 1990 | 2297667 |
a prospective study of viral and mycoplasma infections in chronic inflammatory bowel disease. | seventy-two children with chronic inflammatory bowel disease were investigated for infections with various viruses and mycoplasma pneumoniae, chlamydia psittaci, and coxiella burnetii to determine whether these pathogens are associated with acute onset exacerbations. altogether 54 infections were identified serologically, of which 23 (42.6%) were associated with exacerbations. this corresponded to 24.2% of the recorded exacerbations during the study period. the respiratory pathogens accounted fo ... | 1990 | 2298361 |
tropical arthritis in papua new guinea: a reactive arthritis. | an idiopathic, asymmetrical oligoarthritis affecting young adults is prevalent in the tropical regions of africa, asia and melanesia. a serological study was undertaken in 23 consecutive patients with polyarthritis. each patient was assigned two paired control subjects. a standardized history, physical examination and investigations were performed. acute and convalescent sera were examined for evidence of recent infection. the presence of hla-b27 was determined. twelve (52%) of the 23 patients h ... | 1990 | 2300025 |
a nonsense mutation causing decreased levels of insulin receptor mrna: detection by a simplified technique for direct sequencing of genomic dna amplified by the polymerase chain reaction. | mutations in the insulin receptor gene can render the cell resistant to the biological action of insulin. we have studied a patient with leprechaunism (leprechaun/minn-1), a genetic syndrome associated with intrauterine growth retardation and extreme insulin resistance. genomic dna from the patient was amplified by the polymerase chain reaction catalyzed by thermus aquaticus (taq) dna polymerase, and the amplified dna was directly sequenced. a nonsense mutation was identified at codon 897 in exo ... | 1990 | 2300553 |
immunoblastic lymphoma of t-cell type in a chronically immunosuppressed renal transplant recipient. | a case of non-hodgkin's lymphoma of t-cell type occurring in a renal transplant recipient is described. this lymphoma was classified as large cell, immunoblastic type and presented as a mediastinal mass, although it was demonstrated to be disseminated at autopsy two weeks later. lymphoma cells expressed the immunologic profile of a mature, activated cytotoxic/suppressor t-lymphocyte. t-cell receptor gene rearrangement and karyotypic studies confirmed a clonal t-cell genotype. molecular studies f ... | 1990 | 2301290 |
non-promoting diterpene esters can induce epstein-barr virus early antigen expression in the raji cell line. | a range of diterpene ester ligands with selective biological activity (e.g., irritant but not tumour promoting) were tested for their ability to induce epstein-barr virus (ebv) early antigen expression in the lymphoblastoid raji cell line. all c12/c13 substituted compounds were found to be capable of inducing some antigen expression at nm-microm levels, including desacetyl-alpha-sapinine, a compound largely devoid of biological activity. the non-promoting, fluorescent compound, sapintoxin a, was ... | 1990 | 2302694 |
appearance of bisected n-acetylglucosamine residue of biantennary sugar chains and decrease of high molecular weight oligosaccharides of human lymphocytic cell membranes during differentiation. | asparagine-linked sugar chains obtained from the plasma membranes of human acute lymphocytic leukemic cells, human b cells, epstein-barr virus (ebv)-infected b cell lines, and t cells were quantitatively liberated from the polypeptide portions by hydrazinolysis followed by n-acetylation and nab3h4 reduction. the radioactive oligosaccharides were fractionated by high-voltage paper electrophoresis. their structures were studied by column chromatography and sequential exoglycosidase digestion. the ... | 1990 | 2302846 |
production of baboon (papio hamadryas) monoclonal antibodies by herpesvirus papio immortalized baboon lymph node cells. | baboon monoclonal antibodies specific for monomorphic determinants of baboon apolipoprotein b were produced from in vivo primed lymph node cells immortalized by herpesvirus papio (hvp). two cell lines from the immortalized cells were readily stabilized and grew rapidly. since epstein-barr virus (ebv) cannot be used to immortalize baboon or macaque cells, hvp expands the potential sources of monoclonal antibodies to include baboons, and is the immortalizing agent of choice for macaques as well. | 1990 | 2303734 |
unusual presentations of lymphoma of the head and neck in childhood. | lymphoma of the head and neck in children can pose a significant diagnostic problem, especially when histologic analysis indicates non-hodgkin's lymphoma and the initial site of involvement is extranodal. this report describes 15 pediatric cases of lymphoma seen from 1981 to 1987 with an initial presentation in the head and neck. cervical lymph nodes represented the initial site of involvement in 10 of the cases. the other five cases presented with disease in the tonsillar fossa; maxillary sinus ... | 1990 | 2319881 |
gamma delta t cell receptor-bearing lymphocytes during epstein-barr virus infection. | lymphocytes bearing gamma delta t cell receptors (tcr) constitute a minor subpopulation of human peripheral blood lymphocytes. their role and function during microbial infections are largely unknown. in 10 patients with epstein-barr virus-induced infectious mononucleosis, the gamma delta tcr-expressing t cell population expanded during the acute phase. these cells were largely delta tcs1-, cd4-, and cd8- but expressed activation antigens such as human leukocyte antigen-dr and cd38. the convalesc ... | 1990 | 2324529 |
prospective study of the occurrence of monoclonal gammapathies following bone marrow transplantation in young children. | we have prospectively studied the occurrence of monoclonal serum immunoglobulins in 38 recipients of bmt. patients were young children with primary immunodeficiencies (n = 31), other inherited diseases (n = 4), leukemia (n = 2), or aplastic anemia (n = 1). twenty-nine received an hla-nonidentical marrow and nine an hla-identical marrow. serum monoclonal immunoglobulins were detected by the immunofixation method. monoclonal immunoglobulins were found in 26 patients. monoclonal components were mor ... | 1990 | 2326867 |
immunoreactive growth hormone (gh) secretion by human lymphocytes: augmented release by exogenous gh. | peripheral blood mononuclear cells (pbmcs) from normal adults secreted small amounts of human growth hormone (gh; 0.2-0.6 pg/10(5) cells/7 days culture) as measured by a highly sensitive enzyme immunoassay. stimulation of pbmcs with phytohemagglutinin (pha) consistently showed a 4-6 fold increase in gh secretion. transformed b-lymphocytes by epstein-barr virus also secreted gh (0.8-4.8 pg/5 x 10(4) cells/7 days culture). gh secreted by lymphocytes comigrated with pituitary gh on an ultrogel aca4 ... | 1990 | 2334410 |
mab 104, a new monoclonal antibody, recognizes the b7 antigen that is expressed on activated b cells and htlv-1-transformed t cells. | a new monoclonal antibody (mab) of the igg1 subclass, mab 104, has been obtained after immunization of mice with the burkitt lymphoma cell line jijoye. it only weakly binds to a small proportion of non-activated normal b cells and binds to a larger proportion of in vitro-activated normal b cells. all tested epstein-barr virus (ebv)-transformed b-cell lines, burkitt lymphoma cell lines and freshly isolated follicular b-lymphoma cell preparations strongly bound mab 104. mab 104 did not bind to per ... | 1990 | 2335374 |
development of multiple monoclonal serum immunoglobulins (multiclonal gammopathy) following both hla-identical unfractionated and t cell-depleted haploidentical bone marrow transplantation in severe combined immunodeficiency. | we have identified five patients with severe combined immunodeficiency (scid) who developed multiple monoclonal serum immunoglobulin components (multiclonal gammopathy) following bone marrow transplantation. four patients received haploidentical bone marrow stem cells depleted of t cells and other mature marrow cells by soy lectin agglutination and/or sheep erythrocyte rosetting. one patient received unfractionated hla-identical bone marrow. twenty-one distinct paraproteins were detected: 14 igg ... | 1990 | 2338452 |
aftr: a fifth human b-cell-specific surface antigen. | we report the identification and characterization of a fifth b-cell-specific surface antigen (aftr) detected by three murine monoclonal antibodies. aftr is present on all malignant human b-cell lines tested except those of plasma cells and on malignant b cells from 49/49 patients, including those with pre-b-cell acute lymphoblastic leukemia. similarly, aftr is found on all cd19+ b cells from normal bone marrow, peripheral blood, and tonsil, although it is only weakly expressed on epstein-barr vi ... | 1990 | 2341301 |
[chronic infection caused by epstein-barr virus and chronic fatigue syndrome]. | | 1990 | 2342394 |
acute psychosis in a patient with epstein-barr virus infection. | a case study is presented of an adolescent female who became psychotic and attempted suicide during an acute epstein-barr virus infection. resolution of the infection resulted in the patient returning to her previous normal mental status. the adolescent's ingestion of acetaminophen masked the signs and symptoms of infection and delayed proper diagnosis. | 1990 | 2347847 |
associations between cerebrospinal fluid parameters and high degrees of anxiety or depression in united states air force personnel infected with human immunodeficiency virus. | the relationship between anxiety and depression, and serum and cerebrospinal fluid (csf) immune parameters was studied in 98 patients randomly selected from those undergoing routine medical evaluation for human immunodeficiency virus seropositivity as part of the u.s. air force's mandatory screening program. a total of 95% of the sample did not have acquired immunodeficiency syndrome (aids). the population was subdivided into high and low anxiety or depression groups for statistical purposes. si ... | 1990 | 2348194 |
diagnosis of nasopharyngeal carcinoma. | the diagnosis of nasopharyngeal carcinoma rests on perceptive symptom analysis, a careful clinical examination, and the selection of appropriate clinical investigations. a high index of suspicion is required. when mirror examination fails to provide a satisfactory view of the nasopharynx, flexible or rigid nasopharyngeal endoscopy is mandatory. serologic examination of iga antibodies to epstein-barr virus early antigen and viral capsid antigen is helpful in identifying high-risk patients. biopsy ... | 1990 | 2351085 |
epstein-barr virus nuclear antigen 2 transactivates latent membrane protein lmp1. | several lines of evidence are compatible with the hypothesis that epstein-barr virus (ebv) nuclear antigen 2 (ebna-2) or leader protein (ebna-lp) affects expression of the ebv latent infection membrane protein lmp1. we now demonstrate the following. (i) acute transfection and expression of ebna-2 under control of simian virus 40 or moloney murine leukemia virus promoters resulted in increased lmp1 expression in p3hr-1-infected burkitt's lymphoma cells and the p3hr-1 or daudi cell line. (ii) tran ... | 1990 | 2352328 |
fast gel electrophoresis to analyze dna-protein interactions. | a rapid method for electrophoresis of dna-protein complexes is described. popular "gel-shift" assays are performed using pharmacia phastsystem with its convenience of pre-cast gels and buffer strips and microprocessor-controlled high-resolution separation. using this system, the products of a dna binding reaction (dna-protein complexes) can be separated from "free" dna in less than one hour. dna fragments as well as oligonucleotides have been used as targets with partially purified extracts cont ... | 1990 | 2357378 |
[establishment of a novel cell line derived from nasopharyngeal carcinoma]. | a novel epithelial cell line (designated as hne-1), derived from nasopharyngeal carcinoma (npc), was established and has passed more than 100 generations over one year. the npc biopsy specimen was taken from a 27 year old man with poorly differentiated squamous cell carcinoma of the nasopharynx. the cultured cells showed polygonal shape and grew into multilayers under the inverted microscope. electron microscopy showed that hne-1 cells were characterized by bi-directional differentiation with so ... | 1990 | 2364868 |