| [evaluation of imipenem/cilastatin sodium in the treatment of respiratory tract infections]. | imipenem/cilastatin sodium (ipm/cs) was administered to 55 patients with respiratory tract infections (rti). a clinical evaluation of ipm/cs was carried out in 51 patients, 28 with pneumonia, 4 with pulmonary abscess, 1 with pyothorax, 6 with bronchitis, 9 with bronchiectasis, 1 with diffuse panbronchiolitis and 2 with rti with chronic obstructive pulmonary disease, and the clinical efficacy rate was 78.4%. causative organisms were isolated in 23 strains out of 20 patients, such as staphylococcu ... | 1990 | 2348550 |
| comparative activity of human and murine tumor necrosis factor in toxicity and anti-infectious assays in mice. | in comparative experiments, the lethal effect of rhutnf or rmutnf was evaluated in animals sensitized by adrenalectomy or galactosamine treatment. no clear-cut difference was observed in the effective dose irrespective of the origin of the tnf preparation in swiss or in c3h/hej mice. the latter mouse substrain known to be a low responder to lps was used in the assays to evaluate the influence of lps contamination in tnf-induced responses. like lps, tnf was shown to induce abortion in pregnant mi ... | 1990 | 2348779 |
| klebsiella metastatic endophthalmitis--a complication of shock wave lithotripsy. | a 70-year-old man suffering from nephrolithiasis developed acute pyelonephritis following extracorporeal shock wave lithotripsy of renal stones. after 10 days, urosepsis complicated by meningitis was diagnosed, and 72 hours later he developed right panophthalmitis with perforation of the globe, which was treated by enucleation. a week later, while on cefotaxime and cefuroxime, a focal infectious process developed in the retina of the left eye's posterior pole, spreading into the vitreous and cau ... | 1990 | 2348968 |
| early versus late onset neonatal septicemia at children's hospital. | an analysis was made of 695 cases of neonatal sepsis at children's hospital from 1982 to 1986. the incidence of neonatal sepsis and septicemia were 6.5 and 2.4 per 1,000 livebirths respectively. there were 178 cases of septicemia with onset during the first four days of life (early onset group) and 77 cases with onset after four days of life (late onset group). both groups did not differ significantly in sex, birth weight and gestational age. most of the cases had low birth weight and were prema ... | 1990 | 2351897 |
| [the specificity of an immune serum to the exocellular lipopolysaccharide of pseudomonas wieringae]. | the serum obtained to exocellular lipopolysaccharide (elps) of pseudomonas wieringae selectively agglutinated strains of pathovar of p. syringae and did not agglutinated strains of p. cichorii, p. solanacearum, p. gladioli pv. allicola, p. fluoroviolaceus, strains of nonphytopathogenic pseudomonads as well as bacteria of the genera erwinia, bacillus, xanthomonas, klebsiella. consequently, the antigen determinant common with antigen of the species pseudomonas syringae is present in the compositio ... | 1990 | 2352502 |
| norfloxacin use in urinary tract infection by urologists and infectious disease specialists. | one hundred thirty-one patients of urologists and infectious disease specialists were entered into an open trial of norfloxacin in the therapy of urinary tract infections (utis). all patients were evaluable for tolerability and 98 were evaluable for efficacy. the patient population in this study was older (mean age 53 years) and had more underlying urogenital disorders (30%) or recent invasive urologic procedures (20%) than the typical patient population with utis. over 50% of the patients had i ... | 1990 | 2354709 |
| oral enterobacteriaceae in patients with hiv infection. | 160 oral swabs of 149 hiv-infected patients and 168 swabs of 166 controls were cultured to detect a possible colonization of the oral cavity with yeasts and enterobacteriaceae. in 5.0% of the hiv-infected patients and in 4.8% of the controls different species of enterobacteriaceae were found. yeasts were always associated with enterobacteriaceae in the group of hiv-infected patients. clinically, different oral lesions were seen in the study group. a correlation between the nature of these lesion ... | 1990 | 2359041 |
| therapeutic studies of cefepime (bmy 28142) in murine meningitis and pharmacokinetics in neonatal rats. | cefepime (bmy 28142) was compared with ceftazidime, cefotaxime, and moxalactam for efficacy in treating experimental meningitis in mice and neonatal rats. mice were infected intracranially with streptococcus pneumoniae, s. agalactiae, staphylococcus aureus, escherichia coli, klebsiella pneumoniae, and pseudomonas aeruginosa and treated intramuscularly. five- to eight-day-old neonatal rats were injected intracisternally with haemophilus influenzae, s. pneumoniae, and s. agalactiae and treated int ... | 1990 | 2360814 |
| bacterial contamination of reconstituted oral rehydration solution. | the extent and nature of bacterial contamination in oral rehydration solution reconstituted for use by individuals and for group of patients was studied. twenty three volunteers (all qualified doctors) were asked to reconstitute a packet of prepackaged salt in half litre of clean unboiled water obtained from taps at their residence. five ml aliquots of ors were collected at 6, 12 and 24 hours after reconstitution for bacteriologic study. the water used by volunteers to reconstitute the ors as we ... | 1990 | 2361738 |
| bacterial cell shape regulation: testing of additional predictions unique to the two-competing-sites model for peptidoglycan assembly and isolation of conditional rod-shaped mutants from some wild-type cocci. | the two-competing-sites model for peptidoglycan assembly for bacterial cell shape regulation suggests that in rods, bacterial cell shape depends on the balance between two reactions (sites), one responsible for lateral wall elongation and the other responsible for septum formation. the two reactions compete with each other so that no lateral wall can be formed during septum formation and vice versa. when the site for lateral wall elongation overcomes that for septum formation, long rods or filam ... | 1990 | 2361946 |
| the impact of pulmonary infections on the survival of lung cancer patients. | to determine the impact of pulmonary infections on survival in patients with lung cancer, a retrospective review of the records of 121 such patients treated at howard university hospital in washington, dc, was done. there were 77 men and 44 women; 118 were black. the mean age was 63.5 years. forty-three patients had squamous cell carcinoma, 31 had adenocarcinoma, 18 had large cell carcinoma, 19 had small cell carcinoma, and ten were unclassified. the stages were as follows: two patients in stage ... | 1990 | 2364370 |
| plasmid--mediated drug resistance in enterobacteriaceae isolated at gondar hospital. | thirteen randomly selected multiresistant strains of e. coli, klebsiella species and providencia stuartii, isolated from gondar hospital patients, were investigated for the presence of transferable drug resistance. resistance transfer occurred in 10 (77%) of the 13 strains. all the transferable resistances were found to be mediated by plasmids of the incompatibility groups h1, fii.j1 and c. the high frequency of the h1 plasmids observed in klebisella strains, indicates multiresistant nosocomial ... | 1990 | 2364901 |
| [the etiology of acute pneumonias in middle-aged and elderly persons]. | as many as 356 patients afflicted with acute pneumonias (of these, 225 were from 60 to over years of age) underwent examinations according to the program including bacteriological analysis of the mucus and detection of antigens and specific antibodies in the blood serum. the etiology of acute pneumonia was established in 346 patients (97.2%). in the etiological structure of acute pneumonia, there prevailed bacteria, among which pneumococci, streptococci and hemophilic bacilli occurring both in t ... | 1990 | 2367992 |
| the gene for klebsiella bacteriophage k11 rna polymerase: sequence and comparison with the homologous genes of phages t7, t3, and sp6. | we determined the nucleotide sequence of gene 1 of klebsiella phage k11, which is a member of the t7 group of phages. the largest open reading frame corresponds to a polypeptide with 906 amino acids and a molecular weight of 100,383 daltons. the deduced amino acid sequence of this polypeptide shows 71% homology to the t7 rna polymerase (the product of t7 gene 1), 72% homology to the t3 rna polymerase and 27% homology to the sp6 rna polymerase. divergent evolution was clearly most pronounced in t ... | 1990 | 2370850 |
| false positive mononucleosis screening test results associated with klebsiella hepatic abscess. | false positive heterophile test results for infectious mononucleosis are rare but may lead to diagnostic confusion. a patient with klebsiella hepatic abscess is described in whom a persistently false positive heterophile test resulted in delayed appropriate diagnosis and therapy. | 1990 | 2371976 |
| effect of organic amendments on bacterial multiplication in lake water. | in cayuga lake water amended with 30 micrograms of glucose or amino acids per ml, an added strain of pseudomonas fluorescens and indigenous bacteria grew extensively, pseudomonas sp. b4 and two rhizobia multiplied at a moderate extent, and introduced escherichia coli and klebsiella pneumoniae multiplied but to only a slight degree. the amendments did not enhance growth of micrococcus flavus and arthrobacter citreus, and an asporogenous strain of bacillus subtilis decreased in numbers. the pseudo ... | 1990 | 2372214 |
| prophylactic and therapeutic effects of a monoclonal antibody to tumor necrosis factor-alpha in experimental gram-negative shock. | a monoclonal antibody to recombinant murine tumor necrosis factor-alpha (tnf alpha), tn3-19.12, was used to explore pathogenetic mechanisms and therapeutic strategies in gram-negative shock. in mice receiving an ld90 dose of escherichia coli o111, tn3-19.12 prevented death if given 1.5 h before or 30 min after challenge. less protection was conferred if the antibody was given 2.5 h after challenge. in control mice receiving an irrelevant antibody, l2-3d9, tnf alpha levels rose (less than or equa ... | 1990 | 2373872 |
| bacteria from solid tumours. | bacteria were grown from 63 (69%) of 91 specimens from necrotic tumours in 63 patients. of the tumours, 14 were abdominal, 5 pelvic, 23 of the head and neck, 4 of the lungs, 4 mediastinal, 2 lymphatic, 3 of the breast, and 8 were miscellaneous. aerobic or facultatively anaerobic bacteria only were present in 12 (19%) specimens, anaerobes only in 10 (16%), and mixed aerobic and anaerobic bacteria in 41 (65%). a total of 83 anaerobic and 47 aerobic and facultatively anaerobic bacteria were isolate ... | 1990 | 2374159 |
| [clinical and pharmacokinetic studies on aztreonam in neonates]. | clinical and pharmacokinetic studies on aztreonam (azt) were performed in neonates. the results are summarized as follows: a total of 6 cases consisting of 5 mature and 1 low-birth-weight infants was clinically evaluated. azt 20 mg/kg was administered 2-3 times daily, via 1 hour intravenous drip infusion for 6-21 days. concomitantly, vancomycin (vcm) 15 mg/kg was administered to 1 case 3 times daily, via 1 hour intravenous drip infusion for 3 days and ampicillin (abpc) 20-50 mg/kg to 3 cases 3 t ... | 1990 | 2374292 |
| [studies of aztreonam in neonates]. | absorption and excretion of aztreonam (azt) in neonates were studied and its clinical evaluation in 10 cases of neonates was performed using 1 hour intravenous drip infusion. 1. serum concentrations of azt in 7 neonates younger than 11 days of age were lower than those in infants. 2. serum concentrations of azt in 5 neonates given 20 mg/kg reached their peaks at the end of intravenous drip infusion with an average value of 45.8 +/- 10.41 micrograms/ml, and t 1/2 was 2.77 +/- 0.32 hours on the av ... | 1990 | 2374296 |
| [clinical evaluation of aztreonam in neonatal infections]. | serum concentration, urinary excretion and clinical application of aztreonam (azt) were studied as follows: 1. serum concentrations of azt 1 hour after intravenous injection were 21.0 micrograms/ml in 1 case administered with approximately 10 mg/kg drug and 44.2 micrograms/ml on the average for 7 cases given approximately 20 mg/kg, indicating that serum concentrations are dose-dependent. average serum half-life in 3 mature babies was 4.75 hours and that in 4 premature babies was 6.59 hours thus ... | 1990 | 2374299 |
| [cryptosporidium as a co-pathogen in infantile diarrhea and pneumonia]. | the authors describe a case of cryptosporidiosis in a 10 month old immunocompetent infant, who suffered from prolonged diarrhoea and pneumonia. cryptosporidium oocysts, giardia lamblia were detected by using modified kinyoun stain, and klebsiella was identified in the stool culture. according to the clinical presentation and the laboratory data the streptococcus infection is presumed as cause of the pneumonia, but the authors could not exclude the role of cryptosporidium in predisposing and/or c ... | 1990 | 2377365 |
| the use of bacteriophage-mediated depolymerisation in investigations of the structure of the capsular polysaccharide from klebsiella serotype k71. | the structure (1) of the heptasaccharide repeating-unit of the capsular polysaccharide from klebsiella serotype k71 follows from methylation analysis and n.m.r. and mass-spectrometric studies of the oligosaccharides obtained on depolymerisation of the polysaccharide with a bacteriophage-borne endo-rhamnosidase. [formula; see text]. | 1990 | 2379215 |
| a prospective study of long-term use of amikacin in a paediatrics department. indications, administration, side-effects, bacterial isolates and resistance. | amikacin (amikin; b-m) was used as the only aminoglycoside for 18 months in a paediatric department within a general hospital because of high levels of resistance of klebsiella pneumoniae, pseudomonas aeruginosa and enterobacter cloacae isolates to tobramycin, gentamicin and netilmicin. between 1 february 1987 and 31 july 1988, 816 children were treated with a slow intravenous injection at a standardised dose adjusted for weight and age. respiratory disease was present in 35.8% of 537 neonates, ... | 1990 | 2382175 |
| [immunomodulating properties of phospholipids]. | immunomodulating properties of glycerophospholipids were studied. both phosphatidyl ethanolamines and phosphatidyl cholines exhibited similar immunostimulating effects. | 1990 | 2382424 |
| a study of the gram-negative bacterial flora in patients with ankylosing spondylitis. | 1. twenty-eight patients with active definite primary ankylosing spondylitis and fifty-four healthy control subjects were studied. 2. the hla-b27 antigen was found in 75% of patients and 3.7% of controls. 3. fecal samples from these subjects were cultured for gram-negative enteric bacteria on two occasions within one month. positive cultures for klebsiella sp were found in 32.1% of patients and in 22.2% of healthy controls, but this difference was not statistically significant. all other microor ... | 1990 | 2386846 |
| direct introduction of a formamido group into the 7 alpha (6 alpha)-position of cephalosporins (penicillins). | a novel direct introduction of a formamido group into the 7 alpha (6 alpha)-position of cephalosporins (penicillins) was achieved by treatment of 7 beta (6 beta)-[(3,5-di-tert-butyl-4-oxo-2,5-cyclohexadien-1- ylidene)methylimino]cephem (penam) esters with n,n-bis(trimethylsilyl)formamide, followed by deblocking with girard reagent t to give the corresponding 7 alpha (6 alpha)-formamido-7 beta (6 beta)-amino derivatives. three 7 alpha-formamidocephalosporins were prepared by the conventional n-ac ... | 1990 | 2387776 |
| klebsiella pneumonia with lung abscess. | | 1990 | 2387832 |
| [clinical examination of ceftibuten in acute bronchitis]. | clinical evaluation of ceftibuten (cetb, 7432-s) was performed in 20 patients with acute bronchitis. they were consisted of 10 males and 10 females aged from 20 to 80 years old. cetb was given orally in daily dose of 300 mg (18 cases) or 600 mg (2 cases) in three divided portions. the duration of administration was 3 to 14 days. especially they were given for 7 days in 16 cases. a total of 11 strains comprising 4 strains of staphylococcus aureus, 2 strains of beta-streptococcus and 1 strain each ... | 1990 | 2391747 |
| [laboratory and clinical studies on norfloxacin in pediatrics]. | norfloxacin (nflx, am-715) is a new quinolone antibacterial agent. in the present study, we carried out laboratory and clinical investigations on nflx in the field of pediatrics. the obtained results are summarized as follows. 1. nflx was very active against escherichia coli, salmonella sp., klebsiella sp., enterobacter sp. and pseudomonas aeruginosa among gram-negative rods. antibacterial activities of nflx against gram-positive cocci were lower than those against gram-negative rods but superio ... | 1990 | 2391753 |
| [bacteriological and clinical studies on norfloxacin in the field of pediatrics]. | bacteriological and clinical studies on norfloxacin (nflx) were carried out in the field of pediatrics, and the results obtained are summarized as follows: 1. the mics of nflx against clinically isolated organisms were determined to compare with mics of nalidixic acid, amoxicillin, cefaclor, erythromycin and fosfomycin. nflx had low mics against both gram-positive and gram-negative bacteria and, particularly, showed higher antimicrobial activity to escherichia coli, salmonella sp., klebsiella pn ... | 1990 | 2391755 |
| aerobic and anaerobic microbiology of paronychia. | pus specimens from 28 patients with paronychia of the finger yielded bacterial growth by techniques for cultivation of aerobic and anaerobic bacteria. anaerobic and aerobic organisms only were isolated in pure culture in five (18%) and eight patients (29%), respectively; mixed aerobic and anaerobic flora were present in 15 patients (54%). seventy-two isolates were recovered, or 2.6 isolates per specimen. the predominant anaerobic organisms were gram-positive anaerobic cocci, bacteroides species, ... | 1990 | 2393186 |
| infection in the elderly. | one hundred and eleven patients with pyogenic infections were studied. of these, 32 patients (28.8%) were diabetic, and 33 patients (30%) had positive cultures obtained. fifty-eight patients (52.3%) had fever (t degrees greater than 37.2 degrees c) while 69 patients (62.2%) had leucocytosis. forty-one patients (36.9%) had nosocomial infections. it was found that patients older than 75 years-old did not respond to infection by a febrile response as often as those younger than 75 years-old. mortal ... | 1990 | 2393238 |
| crossover assessment of serum bactericidal activity and pharmacokinetics of five broad-spectrum cephalosporins in the elderly. | to better define the pharmacokinetics and serum bactericidal activity (sba) of the expanded-spectrum cephalosporins in the elderly, we administered single 2-g intravenous infusions of cefoperazone, cefotaxime, ceftriaxone, ceftazidime, and ceftizoxime to six healthy volunteers over the age of 65 years. serum was collected over 24 h, and concentrations were determined by high-performance liquid chromatography; pharmacokinetic parameters were determined for each drug. sba was measured against repr ... | 1990 | 2393258 |
| deep neck infections in patients at risk for acquired immunodeficiency syndrome. | the acquired immunodeficiency syndrome epidemic has introduced an increasing population of immune-compromised patients susceptible to infections by unusual pathogens. to evaluate the role of traditional antibiotic therapy for deep neck infections in high-risk patients, we reviewed the records of 73 patients with deep neck infections. forty-six had or were at risk for acquired immunodeficiency syndrome, and nine were diabetics. staphylococcal infections were more common in the at-risk group, and ... | 1990 | 2395398 |
| nucleotide sequence of the gene encoding the shv-2 beta-lactamase (blashv-2) of klebsiella ozaenae. | | 1990 | 2395654 |
| comparison between bactec nr. 660 and a conventional 12-tube blood culture system. | the detection power of the automated blood culture system bactec nr 660, based on infrared detection of carbon dioxide in an agitated aerobic medium and a non-agitated anaerobic medium, was compared with that of our conventional 12-tube blood culture system. of 1685 paired blood cultures, 258 (15.3%) were positive in one or both systems. clinically relevant isolates were found in 11.5%. the dominating species were escherichia coli(41%), followed by staphylococcus aureus(14%) and klebsiella spp.( ... | 1990 | 2397114 |
| [antibiotic-resistant klebsiella from coastal waters of the caspian and baltic seas]. | antibiotic resistant klebsiella spp. were found to be widely distributed in coastal waters of seas to which sewage was discharged: 69.9 and 85.9%, in the waters of the baltic and caspian seas, respectively, the number of the strains with multiple resistance was 18.1 and 19.9%, respectively. among the klebsiella spp. with multiple resistance, 40% from the baltic sea and 58.1% from the caspian sea had conjugative r plasmids. statistically significant regional differences in the content of antibiot ... | 1990 | 2400287 |
| [infections in hematological malignancies--clinical analysis of septic patients admitted to the second department of miyazaki medical college hospital in the past ten years]. | two hundred and sixty-two patients (actual number 162) of hematological malignancies were admitted to our department from november 1977 to december 1986. fourty-three of them (16.4%) were demonstrated to be accompanied with sepsis by blood culture. in acute non-lymphocytic leukemias (aml, apl, amol) the rate of sepsis was 33.8% (27 patients), while in lymphocytic malignancies (ml, hd, atl) it was 11.7% (16 patients), particularly being 3.0% in atl. among the detected pathogenic microorganisms, g ... | 1990 | 2401813 |
| detoxification of plasma containing lipopolysaccharide by adsorption. | we compared the ability of 13 materials, all with known affinity for lipopolysaccharide (lps), to remove lps from water, pooled normal human plasma, and plasma from a patient with gram-negative bacterial sepsis. escherichia coli o111:b4 lps was added to water and pooled normal human plasma to a concentration of 200 ng/ml and aliquots were adsorbed in parallel with each of the materials. plasma containing 25 ng/ml of lps was obtained by plasmapheresis from a patient with fatal klebsiella pneumoni ... | 1990 | 2403508 |
| hydrogen peroxide mediates the oxidative inactivation of enzymes following the switch from anaerobic to aerobic metabolism in klebsiella pneumoniae. | klebsiella pneumoniae utilizes distinct pathways for the anaerobic and aerobic metabolism of glycerol. during anaerobic growth, glycerol is first converted to dihydroxyacetone by glycerol dehydrogenase; subsequent phosphorylation yields dihydroxyacetone phosphate. during aerobic growth, glycerol is initially phosphorylated to yield glycerol 3-phosphate; subsequent reduction then gives dihydroxyacetone phosphate. a coordinated response occurs when anaerobically growing cells are switched to aerob ... | 1990 | 2403562 |
| cephalosporins to carbapenems: 1-oxygenated carbapenems and carbapenams. | the photo "wolff" rearrangement of readily available 2-diazoceph-3-em oxides (1) directly affords carbapen-2-ems, allowing a facile entry into a ring system previously accessible only by total synthesis, lengthly semisynthesis or fermentation. the chirality of the cephalosporin is accurately translated into the corresponding carbapenem. the resulting 1-oxocarbapenems (2) were selectively transformed through reduction into 1-oxygenated carbapenems and carbapenams (3 and 4, respectively). on micro ... | 1990 | 2404120 |
| role of the promoter in activation of transcription by nitrogen regulator i phosphate in escherichia coli. | the protein nitrogen regulator i (nri)-phosphate is known to activate the initiation of transcription of the escherichia coli glna gene. this activation is facilitated by the binding of the protein to nri-specific sites located upstream of the sigma 54-dependent glna promoter. to determine whether binding of nri-phosphate to upstream sites is sufficient for activation, we placed several promoters not normally activated by nri-phosphate downstream of nri binding sites and measured activation in i ... | 1990 | 2404958 |
| klebsiella pneumoniae bacteraemia at an urban general hospital. | of 47 patients with klebsiella pneumoniae bacteraemia admitted to the hillbrow hospital, johannesburg during a period of 18 months, 31 were males and 16 were females. features predisposing to illness were found in 89.4% patients, chronic alcoholism, neoplastic disease and diabetes mellitus being the most common. twenty-five infections were acquired in hospital and 22 in the community. most patients (59.6%) had pneumonia. all isolates of k. pneumoniae were resistant to ampicillin (100%); several ... | 1990 | 2405058 |
| trehalose dimycolate enhances survival of fission neutron-irradiated mice and klebsiella pneumoniae-challenged irradiated mice. | the survival of b6d2f1 female mice exposed to lethal doses of fission neutron radiation is increased when trehalose dimycolate (tdm) preparations are given either 1 h after exposure or 1 day before exposure to radiation. tdm in an emulsion of squalene, tween 80, and saline was the most effective formulation for increasing the 30-day survival of mice when given 1 day before (90%) or 1 h after (88%) exposure to radiation. an aqueous suspension of a synthetic analog of tdm was less effective at inc ... | 1990 | 2405452 |
| demonstration of lipid a-binding proteins on murine lymphoma cells using r-mutant gram-negative bacteria as a detector. | some r-mutant escherichia coli and salmonella heavily adhered to murine lymphoma cells of b cell and t cell lineages. this adhesion was primarily mediated by membrane-localized proteins on tumor cells, which bind the polymyxin b-reactive hydrophilic structure of lipis a on bacteria. sds-page analysis of tumor cell membranes showed that proteins or glycoproteins of mw = around 45kd, 25-35kd and around 15kd preferentially bind lipid a. various lymphoma cell lines binding the bacteria at different ... | 1990 | 2405889 |
| sepsis of vascular catheters. ii: in vitro disinfection of colonized tubing. | preparatory to development of in situ disinfection of implanted catheters, silicone rubber tubing colonized by incubation with staphylococcus aureus, staphylococcus epidermidis, or klebsiella pneumoniae was used to test the efficacy of various chemicals in vitro. protocols sterilizing all segments colonized for 24 h (n = 30) were immersion into 50% povidone iodine for 5 and 60 min, 100% povidone iodine for 5, 15, and 60 min, 1.2 x 10(3) ppm chlorine dioxide for 15 and 60 min, and 1.2 x 10(3) ppm ... | 1990 | 2406273 |
| bacterial conversion of glycerol to beta-hydroxypropionaldehyde. | beta-hydroxypropionaldehyde (3-hpa) can be oxidized to acrylic acid, an industrially important chemical used in the manufacture of synthetic plastics and other polymers. of 19 genera and 55 strains tested, 3 klebsiella and 2 enterobacter strains produced 3-hpa. the most efficient strain was klebsiella pneumoniae nrrl b-4011. under optimum conditions (28 degrees c; 40 g of semicarbazide hydrochloride per liter, 70 g of glycerol per liter; and ph 6.0), 3.1 g of b-4011 cells per liter accumulated 2 ... | 1990 | 2407183 |
| essential histidine residues in coenzyme b12-dependent diol dehydrase: dye-sensitized photooxidation and ethoxycarbonylation. | the apoenzyme of diol dehydrase was inactivated by photoirradiation in the presence of rose bengal or methylene blue, following pseudo-first-order kinetics. the inactivation rates were markedly reduced under a helium atmosphere, suggesting that the inactivation is due to photooxidation of the enzyme under air. the half-maximal rate of methylene blue-sensitized photoinactivation was observed at ph around 7.5. amino acid analyses indicated that one to two histidine residues decreased upon the dye- ... | 1990 | 2407195 |
| cholecystopleural fistula with cholelithiasis presenting as a right pleural effusion. | at autopsy, multiple gallstones were recovered from the right pleural space of an elderly patient who presented with a massive right pleural effusion and septic shock. the mechanisms of gallstone migration and fistula formation between the gallbladder and right pleural space are described. despite atypical presentations, gallbladder disease remains an important differential consideration of right pleural effusion in the elderly. | 1990 | 2407457 |
| comparative chemotherapeutic activity of cefpirome and imipenem in experimental infections. | in systemic and local infections, the therapeutic efficacy of cefpirome was compared to that of imipenem and cefotaxime. murine septicemia induced with methicillin-sensitive and methicillin-resistant staphylococcus aureus strains responded well to cefpirome and imipenem therapy, the ed50 values ranged from 0.8 to 28.40 mg/kg and 0.5 to 15.58 mg/kg, respectively. the carbapenem also displayed high efficacy against enterococci and was more potent than cefpirome. cefotaxime, however, exhibited lowe ... | 1990 | 2407707 |
| 6-(substituted methylene)penems, potent broad spectrum inhibitors of bacterial beta-lactamase. ii. racemic furyl and thienyl derivatives. | a series of racemic 6-(substituted methylene)penems have been prepared. these compounds contain a 5-membered monoheteroaromatic ring at c-8. the antibacterial/synergistic and beta-lactamase inhibitory activities of both e- and z-isomers and 2-substituted derivatives are compared. | 1990 | 2407709 |
| comparison of the exoproducts of gram-negative bacteria by sds-page. | the protein exoproducts released during exponential growth of gram-negative bacteria were analysed and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the following bacterial strains were tested: escherichia coli, klebsiella pneumoniae, citrobacter freundii, enterobacter cloacae, serratia liquefaciens, serratia rubidaea, proteus mirabilis, proteus vulgaris, salmonella minnesota, pseudomonas aeruginosa and pseudomonas fluorescens. it is demonstrated by sds-page t ... | 1985 | 2408403 |
| microcin e492, a low-molecular-weight peptide antibiotic which causes depolarization of the escherichia coli cytoplasmic membrane. | microcin e492 is a 5,000- to 7,000-molecular-weight peptide antibiotic which depolarizes the cytoplasmic membrane of sensitive escherichia coli k-12 cells. the microcin has many features in common with colicin v but is distinguished from it by the absence of microcin e472 immunity in colv+ strains and by the fact that colicin v-insensitive cir mutants of e. coli k-12 are microcin sensitive. neither of the two plasmids detected in the producing strain of klebsiella pneumoniae appear to carry the ... | 1985 | 2408563 |
| adjuvant actions of polyclonal lymphocyte activators. v. proliferation of macrophage colony-forming cells in the draining lymph node. | we investigated the action of various polyclonal lymphocyte activators (pla) on the proliferation of macrophage colony-forming cells in vivo at the local site. as pla, klebsiella pneumoniae 03 lipopolysaccharide (k03 lps), escherichia coli 0111 lipopolysaccharide (e. coli lps), dextran sulfate (ds), concanavalin a (con a), phytohemaggulutinin (pha), polyadenylic-polyuridylic acid (poly(a:u], polyinosinic-polycytidylic acid (poly(i:c], and pokeweed mitogen (pwm) were used. all pla tested acted to ... | 1985 | 2408767 |
| a heat-modifiable outer membrane protein carries an antigen specific for the species yersinia enterocolitica and yersinia pseudotuberculosis. | the outer membranes of gram-negative bacteria are considered to be of importance in host-bacteria interaction, in protective immunity, and occasionally in subclassification within a species. in this study, the outer membranes of several strains of yersinia enterocolitica and y. pseudotuberculosis were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). it was found that the appearance of the major proteins depended on the temperature at which they were solubilized i ... | 1985 | 2409152 |
| slo4, a new interferon inducer isolated from klebsiella pneumoniae and escherichia coli. | a product isolated from klebsiella pneumoniae and escherichia coli, coded slo4, has been shown to be effective in endogenous interferon induction in vivo in mouse when administered ip or iv, and in vitro with human leukocyte cultures. in these two systems induced interferon was defined. the inducer has not yet been characterized but seems not to belong to any components known to be interferon inducers such as viral particles, nucleic acids or endotoxins. an analytical study will be carried out t ... | 1985 | 2409177 |
| [protective action of a corpuscular polyvalent pseudomonas aeruginosa vaccine against representative enterobacteria]. | animal experiments have demonstrated that p. aeruginosa vaccine is capable of protecting animals from experimental p. aeruginosa infection, as well as rendering a protective effect with respect to some representatives of the family enterobacteriaceae. the comparative study of the antigenic spectra of the vaccine strains and some representatives of enterobacteriaceae (enterobacter, serratia, citrobacter and klebsiella) has revealed no direct relationship between the degree of this protective effe ... | 1985 | 2409719 |
| [study on the prostatic tissue levels of cefmenoxime (cmx)]. | the concentrations of cefmenoxime (cmx) were examined in the serum and prostatic tissue of 25 patients with benign prostatic hypertrophy and of 1 patient with prostatic carcinoma. the cmx levels were measured at scheduled intervals after 2 g cmx administration by one hour drip infusion prior to prostatectomy. pharmacokinetic analysis was performed based on the two compartment open model theory. 1) maximum serum level of cmx was 124 micrograms/ml at the end of drip infusion and the biological hal ... | 1985 | 2409786 |
| spondyloarthritis, uveitis, hla-b27 and klebsiella. | the association between hla-b27 and ankylosing spondylitis could be explained by a "crosstolerance hypothesis". experimental studies demonstrate immunological crossreactivity between hla-b27 and antigens found in gram-negative bacteria, such as klebsiella. clinical studies show that an increased frequency of patients with ankylosing spondylitis and uveitis have klebsiella in their fecal cultures. furthermore, antibodies to klebsiella can be detected in such patients during active phases of the d ... | 1985 | 2412951 |
| enhanced resistance of mice against influenza virus infection after local administration of glycoprotein extracts from klebsiella pneumoniae. | ru. 41 740, a glycoprotein extract from klebsiella pneumoniae o1k2 strain was tested for its ability to enhance resistance of mice against influenza virus infection. local (aerosol) and systemic (ip) routes of ru. 41 740 administration were compared for their effectiveness in protecting mice. when ru. 41 740 was administered prophylactically (10 mg/kg) via aerosol route (5 consecutive days before challenge), significant protection (p less than 0.0001) was conferred against lethal aerosol inocula ... | 1985 | 2412973 |
| [reactivity to bacterial peptidoglycans in a phagocytosis system. the range of immunological specificity of escherichia coli peptidoglycan]. | the antigenic specificity of e. coli m17 peptidoglycans (pg) was studied in the test system based on the stimulation of neutrophils with pg opsonized with the whole and exhausted igg fraction of human serum. within the generic taxon, the pg under study were similar in their antigenic properties. the opsonic activity of igg exhausted with the preparations of different e. coli strains decreased by 93.2-95.8%. an essential difference of e. coli pg from similar components of other representatives of ... | 1985 | 2413663 |
| changing pattern of neonatal septicaemia in an african city. | the pattern of neonatal septicaemia in a large cosmopolitan african city is presented. comparison of microorganisms isolated in the present study when compared with the results of previous reports from the same institution, show an increase in the incidence of klebsiella and pseudomonas septicaemia. in contrast, escherichia coli infection has decreased appreciably from an incidence of 56.8% to 14.3%. staphylococcus aureus has remained the dominant gram-positive pathogen over the last decade whil ... | 1985 | 2415048 |
| adjuvant actions of linear mannan-possessing lipopolysaccharide (lps) in lps-resistant c3h/hej mice. | immunopotentiation has been demonstrated when klebsiella o3 lipopolysaccharide (ko3 lps), which possesses a linear mannan as the o-specific side chain, was injected subcutaneously into endotoxin resistant c3h/hej mice together with soluble protein antigens. the lps exhibited significantly positive adjuvant effects on antibody responses in vivo after secondary antigen challenge and on delayed-type hypersensitivity (dth) reactions against protein antigens. however, ko3 lps was not a polyclonal b c ... | 1985 | 2416680 |
| [serum and prostatic tissue concentration of ceftizoxime (czx) after intravenous administration in cases of benign prostatic hypertrophy]. | one gram of ceftizoxime (czx) was administrated before transurethral resection in 38 cases of benign prostatic hypertrophy. then the serum and tissue concentration of czx was measured, and compared with the sensitivity of czx to the bacteria separated from the urine of inpatients. the concentration of czx in prostatic tissue was higher than the mic80 for e. coli for 16.7 hr, for klebsiella for 18.7 hr, for proteus for 14.7 hr and for serratia for 8.86 hr. therefore, czx was considered to be an e ... | 1985 | 2418663 |
| symptomatic urinary tract infections in high-risk nigerian newborn infants. | thirty-nine high-risk newborn infants with proven urinary tract infections were studied and compared with 51 control infants. the remarkably low incidence of 0.82/1000 live births and the absence of radiological abnormalities of the urinary tract contrast with previous reports in the literature. septicaemia and necrotizing enterocolitis were more frequently encountered in infants with urinary tract infection. escherichia coli and klebsiella were the predominant pathogens isolated. the male prepo ... | 1985 | 2418764 |
| the influence of the o and k antigens of klebsiella aerogenes on surface hydrophobicity and susceptibility to phagocytosis and antimicrobial agents. | the surface hydrophobicity of klebsiella aerogenes is influenced by the presence of capsular (k) and lipopolysaccharide (o) antigens. loss of both k and o antigens (k-o-), but not the k antigen alone (k-o+), increased surface hydrophobicity and susceptibility to phagocytosis. unheated serum (i.e., containing complement) increased the surface hydrophobicity and phagocytosis of the k-o+ and k-o- strains, but not of the k+o+ encapsulated parent strain. despite the altered susceptibility to phagocyt ... | 1986 | 2419562 |
| [bacteriophage typing of klebsiella. i. role of klebsiella surface structures in bacteriophage binding]. | | 1985 | 2421280 |
| normal anti-klebsiella lymphocytotoxicity in ankylosing spondylitis. | we compared in vitro lymphocytotoxicity (lct) of peripheral blood lymphocytes (pbl), obtained from patients with ankylosing spondylitis (as) and normal controls (nc). assays were performed with antibacterial antisera prepared from as- and nc-derived klebsiella and coliforms escherichia coli. lct assessed by eosin staining was not significantly different in pbl of 12 as patients and 28 controls when reacted with 3 klebsiella and 1 e coli antisera. lct assessed by 51cr release was not significantl ... | 1986 | 2421740 |
| analysis of gene expression in rapidly developing mutants of dictyostelium discoideum. | developmentally regulated gene expression has been analyzed in the wild-type d. discoideum strain nc-4 and a series of temporally deranged mutants. the mutants include representatives from each class of rapid development mutation, fr17(rdea-) and ht506(rdec-), and strain hifm-1, which appears to be defective in the timing of events early in development. we have monitored four prespore-specific genes, three of which show coordinate expression in the wild type. the coordination is maintained in ea ... | 1986 | 2423400 |
| nosocomial klebsiella pneumoniae colonization in a neonatal special care unit. | a clinical study is reported of nosocomial klebsiella pneumoniae colonization and infection in a neonatal special care unit in barbados. of the 14 infants who were affected nine were symptomatic and two died (15%) during a 9-day period. this outbreak appeared to have been an indirect result of an acute shortage of nursing personnel coupled with a breakdown in adequate handwashing techniques. we feel that periodic review and continuous reinforcement of infection control policies in areas with pat ... | 1986 | 2425720 |
| [antibacterial activity of a number of preparations and their combination with a proteolytic enzyme against the microflora of burn and surgical wounds]. | antibacterial activity of antibiotics (azlocillin, tobramycin and piperacillin), antiseptics (metacid, chlorhexidine and quinosoline) and some other drugs (dioxidine and mafenide acetate) against the main representatives of the burn and surgical wound microflora, i. e. staphylococcus, p. aeruginosa, proteus, providencia, serratia, klebsiella and e. coli was studied. tobramycin and piperacillin were highly active against p. aeruginosa, staphylococcus and proteus. it was shown that in the presence ... | 1986 | 2425729 |
| [biotyping and resistotyping for epidemiological tracing of the fecal origin of klebsiella pneumoniae in urinary infections]. | twenty-four (82.7%) out of 29 patients suffering from hospital acquired urinary infections by klebsiella pneumoniae had the same species in their faeces. biotyping of 24 urinary and 219 fecal strains of k. pneumoniae resulted in 50 different biotypes - an average of four biotypes per fecal sample. ten patients (34.4%) had the same biotype in urine and faeces without any correlation with previous vesical catheterization (p greater than 0.05). using resistotyping to four chemical compounds selecte ... | 1985 | 2426557 |
| predictive value of c-reactive protein in neonatal septicaemia. | the following non-specific indices of infection were studied in septicaemic and non-septicaemic babies: haemoglobin, total white blood cell count, differential white count, esr, platelet count, c-reactive protein (crp), serum immunoglobulins, plasma c3 and haptoglobin. forty-three low-birthweight (lbw) infants with clinical features suggesting septicaemia were investigated; blood cultures were positive in 19 and negative in 24. the mortality was 53% in the culture-positive and 13% in the culture ... | 1986 | 2428291 |
| posttranscriptional control of klebsiella pneumoniae nif mrna stability by the nifl product. | posttranscriptional control of nif mrna stability was demonstrated by functional and chemical analyses, using specific probes for four nif transcripts. in the wild type, nif transcripts (except nifla) were stable during derepression, with half-lives of approximately 30 min. they were dramatically destabilized by o2 or elevated temperature (41 degrees c) and to a lesser extent by nh4+. in contrast, the nifla message was not particularly stable, and posttranscriptional control was not evident. in ... | 1986 | 2428807 |
| rapid identification of klebsiella pneumoniae in the lung tissue by fluorescence microscopy. | klebsiella pneumoniae is stained weekly with hematoxylin and eosin, moderately with periodic acid schiff (pas), and strongly by the warthin-starry method. staining by the warthin-starry method, however, takes more than one hour. therefore, we developed a new procedure for rapid identification of k. pneumoniae in paraffin sections of the lung under a fluorescence microscope. | 1986 | 2429402 |
| transcriptional regulation of the spo0f gene of bacillus subtilis. | we have cloned the early sporulation gene spo0f, which encodes an open reading frame of 124 codons. the putative spo0f protein derived from this open reading frame, which has been shown to share homology with the spo0a protein as well as several other regulatory proteins from escherichia coli, salmonella typhimurium, and klebsiella pneumoniae, also shares homology with the e. coli ecori methyltransferase. we have shown by s1 nuclease mapping of in vivo transcripts that spo0f is regulated from du ... | 1986 | 2430944 |
| [enterotoxigenicity of bacteria of the genera klebsiella and enterobacter determined by immunologic methods]. | | 1986 | 2431213 |
| staining of o-specific polysaccharide chains of lipopolysaccharides with ruthenium red. | s-form lipopolysaccharides (lps) from klebsiella strain len-1 (o3: k1-) and from salmonella minnesota strain 1114 were positively stained with ruthenium red, whereas r-form lps from klebsiella strain len-111 (o3-: k1-) and ra, rb1, rcp+, rd1p-, and re lps from the respective mutant strains of s. minnesota were not or only faintly stained by such treatment. from these results it was concluded that ruthenium red stains the o-specific polysaccharide chains of lps. the appearance of stained preparat ... | 1986 | 2431251 |
| analysis of cell division gene ftsz (sulb) from gram-negative and gram-positive bacteria. | the ftsz (sulb) gene of escherichia coli codes for a 40,000-dalton protein that carries out a key step in the cell division pathway. the presence of an ftsz gene protein in other bacterial species was examined by a combination of southern blot and western blot analyses. southern blot analysis of genomic restriction digests revealed that many bacteria, including species from six members of the family enterobacteriaceae and from pseudomonas aeruginosa and agrobacterium tumefaciens, contained seque ... | 1987 | 2432055 |
| solid-state nmr studies of klebsiella pneumoniae grown under nitrogen-fixing conditions. | the carbon and nitrogen metabolism of klebsiella pneumoniae m5a1 has been characterized using 13c and 15n labeling with detection by cross-polarization magic-angle spinning solid-state nmr. cells grown on ammonium typically require some 20 h to derepress fully for nitrogenase when transferred to medium devoid of any source of fixed nitrogen. we have established that during this period some cellular proteins are catabolized with the liberated nitrogen being used for the synthesis of purines neede ... | 1987 | 2432059 |
| an antiserum to a disease-associated factor from the cells of an hla-b27 positive patient with ankylosing spondylitis specifically recognizes an hla-b27 associated determinant. | antiserum raised to a factor elaborated by a lymphoblastoid cell line derived from the peripheral blood cells of an hla-b27 positive patient with ankylosing spondylitis specifically lyses the b27 positive, but not the b27 negative, cells of ankylosing spondylitis patients. the cells of b27 positive and b27 negative normal controls are not lysed. this serum has similar specificity to antisera against cross-reactive bacteria. | 1987 | 2437933 |
| contribution of the mannan o side-chains to the adjuvant action of lipopolysaccharides. | klebsiella 03 lipopolysaccharide (lps) and escherichia coli 09 lps were previously shown to have potent adjuvant activities in augmenting antibody response and delayed-type hypersensitivity to protein antigens. the r form lps extracted from the o-specific polysaccharide-less mutants derived from klebsiella 03 and e. coli 09 strains were characterized by chemical and electrophoretic analyses. the adjuvant activities of the r-lps in augmenting antibody response and dth to protein antigens were muc ... | 1987 | 2438205 |
| autoantibodies to hla b27 in the sera of hla b27 patients with ankylosing spondylitis and reiter's syndrome. molecular mimicry with klebsiella pneumoniae as potential mechanism of autoimmune disease. | ankylosing spondylitis (as) and reiter's syndrome (rs) both show a strong correlation with the hla b27 haplotype. we studied whether sharing of homologous amino acid sequences in the hla b27 antigen with an invading microbe might occur, and if so, what is the biological significance of such homology. in a computer search of the dayhoff data bank, we found a homology of six consecutive amino acids between hla b27.1 antigen residues 72-77 and klebsiella pneumoniae nitrogenase residues 188-193. the ... | 1987 | 2439632 |
| modulation of natural killer activity by muramyl peptides: relationship with adjuvant and anti-infectious properties. | natural killer (nk) activity of spleen cells was studied in dba/2 mice, 24 and 72 h after intravenous injection of various muramyl peptides: muramyl dipeptide (mdp) and derivatives which are both adjuvant-active and able to increase resistance against klebsiella pneumoniae; derivatives which are adjuvant-active but devoid of anti-infectious properties; derivatives which are anti-infectious but devoid of adjuvant activity, and derivatives which are devoid of both activities such as the stereoisom ... | 1987 | 2439906 |
| non-ige-dependent bacteria-induced histamine release from human lung and tonsillar mast cells. | a wide spectrum of formalin-killed bacteria have been tested for their ability to release histamine from human dispersed lung and tonsillar mast cells. escherichia coli, enterobacter cloacae, staphylococcus epidermidis, proteus vulgaris, klebsiella oxytoca and k. pneumoniae were the most effective histamine releasers. further studies on tonsillar mast cells showed that e. coli-induced histamine release differed from ige-dependent release with respect to its kinetics, temperature and ph profiles ... | 1987 | 2441900 |
| acute illness in nigerian children with sickle cell anaemia. | the pattern of illness in 60 consecutive children with homozygous sickle cell disease who attended the paediatric emergency room of a busy lagos hospital with acute illness was studied prospectively. their ages ranged from 3 months to 13 years with a peak in the 2nd year. there were twice as many boys as girls. the commonest symptoms were fever, limb or abdominal pain and cough, and the commonest signs were pallor and hepatomegaly. painful crises occurred in 27, anaemic crises in 11, and a combi ... | 1987 | 2445266 |
| polysaccharide antigens of escherichia coli. | the major surface antigens of escherichia coli are the cell wall lipopolysaccharides (lps; o antigens) and the capsular polysaccharides (ps; k antigens). these polysaccharides are synthesized at the cytoplasmic membrane of the bacteria; the lps are transported to the outer membrane, where they reside, whereas the ps are secreted into capsules. the lps consist of lipid a covalently linked to the core oligosaccharide, which itself is covalently linked to the o-specific polysaccharide. the latter, ... | 1987 | 2446369 |
| [a study of prostatic tissue levels of cefbuperazone]. | the concentration of cefbuperazone (cbpz) were determined by bioassay in the serum and prostatic tissue of 36 patients with benign prostatic hypertrophy, who underwent transurethral resection. one gram of cbpz was injected intravenously prior to surgery. pharmacokinetic analysis was performed using the two or three compartment model theory. the maximum serum level of cbpz was 82.2 micrograms/ml at 30 min after the start of cbpz administration and biological half-life was 97.3 min. cbpz concentra ... | 1987 | 2447766 |
| role of eicosanoids, histamine, and serotonin in the pathogenesis of klebsiella pneumoniae-induced bovine mastitis. | by inoculating klebsiella pneumoniae into the teat canals of mammary glands, coliform mastitis was induced experimentally in 6 lactating cows. release of eicosanoids, histamine, and serotonin in plasma and milk was studied in response to 2 doses of k pneumoniae. a low dose (mean, 5,000 organisms/ml) was inoculated into cows 1 through 4, and a high dose (mean, 200,000 organisms/ml) was inoculated into cows 5 and 6. milk and blood samples were collected before inoculation (0 hours), and hourly, fr ... | 1987 | 2449101 |
| inhibition of human neutrophil elastase by bacterial polyanions. | we previously demonstrated that pneumococcal extracts contain a highly specific inhibitor of human neutrophil elastase (hne). we now show that the active inhibitor in these extracts is a high-molecular-weight, heat-stable substance that appears to be rna, since inhibitory activity of pneumococcal extracts is decreased by incubation with ribonuclease but not by incubation with deoxyribonuclease or proteinase k. moreover, metabolically labeled ([3h]uridine) pneumococcal rna, isolated by phenol ext ... | 1988 | 2449347 |
| bacteriological quality of raw human milk: effect of storage in a refrigerator. | eighty-seven breast milk samples were obtained from 63 mothers of infants on the neonatal intensive care unit of the lagos university teaching hospital. the samples were cultured for bacteria immediately after collection (0 h) and then stored in a domestic refrigerator from where cultures were repeated at 6-hourly intervals for 24 h. at 0 h, three (3.4%) of the samples were sterile; 56 (64%) grew coagulase negative staphylococci, and one (1.1%) streptococcus viridans. thus, 60 (69%) of the sampl ... | 1987 | 2449844 |
| nucleotide sequence and genetic analysis of the nifb-nifq region from azotobacter vinelandii. | a 3.8-kilobase-pair ecori fragment which corrects the mutations carried by the nifb- azotobacter vinelandii strains ca30 and uw45 was cloned, and its nucleotide sequence was determined. four complete open reading frames (orfs) and two partial orfs were found. the translation product of the first partial orf is the carboxy-terminal end of a protein homologous to the nifa gene product from klebsiella pneumoniae. a 285-base-pair sequence containing a potential nif promoter and nif regulatory sites ... | 1988 | 2450865 |
| [effects of human gamma globulin preparations and monoclonal antibody on the whole blood chemiluminescence response]. | | 1987 | 2450934 |
| pore formation by pho-controlled outer-membrane proteins of various enterobacteriaceae in lipid bilayers. | the structural genes of the phoe porins of klebsiella pneumonia, enterobacter cloacae and escherichia coli c, cloned in multicopy plasmids, were transfered into a porin-deficient e. coli k-12 strain, which was constitutive for the pho regulon, and the phoe porins were isolated and purified. phoe of salmonella typhimurium could not be cloned but was isolated from a pho-constitutive strain. reconstitution experiments with artificial lipid bilayer membranes showed that the different phoe proteins f ... | 1988 | 2453363 |
| the sodium ion translocating oxalacetate decarboxylase of klebsiella pneumoniae. sequence of the biotin-containing alpha-subunit and relationship to other biotin-containing enzymes. | the gene encoding the alpha-subunit of the na+ pump oxalacetate decarboxylase of klebsiella pneumoniae was cloned and sequenced. the deduced primary structure of the protein was confirmed by protein sequencing of about 30% of the polypeptide chain. the gene has a gc content of 67% and codes for 596 amino acids. the n-terminal methionine is removed in the mature protein which has a calculated molecular mass of 63,600 daltons. the protein consists of two different domains that are connected by a s ... | 1988 | 2454915 |
| production and characterisation of mouse monoclonal antibodies reacting with the lipopolysaccharide core region of gram-negative bacilli. | monoclonal antibodies to the lipopolysaccharide (lps) core region were produced by immunising mice with escherichia coli strain j5 (chemotype rc). one of these bound to the deepest part of the core, i.e., lipid a, and reacted with other heat-killed but not live gram-negative bacilli, including e. coli, klebsiella pneumoniae and pseudomonas aeruginosa. eight other monoclonal antibodies, binding to the terminal glucose residue of rc lps, reacted with live cells of e. coli strains only. thus, the o ... | 1988 | 2455054 |
| klebsiella and ankylosing spondylitis. | | 1988 | 2456172 |
| bacterial lysis of fibrin seal in vitro. | we performed an in vitro study to determine whether certain bacteria may lyse a commercially available fibrin seal (beriplast, behringwerke, marburg, frg). fibrinogen solution was mixed with actively growing bacterial cultures, and thrombin was added. during 20 days of incubation at 37 degrees c complete lysis was observed with a number of different bacteria, however, at different rates. complete lysis within 1-5 days was observed for the following species (number of strains in parentheses): str ... | 1988 | 2456934 |
| characterization of two genes encoding antigenically distinct type-1 fimbriae of klebsiella pneumoniae. | a uropathogenic isolate of klebsiella pneumoniae was shown to exhibit a mannose-sensitive hemagglutinating phenotype and to produce type-1 fimbriae consisting of subunits with a different electrophoretic mobility than those previously investigated. the gene cluster encoding expression of fimbriae was cloned and the genetic organization of the encoded polypeptides was determined. the gene encoding the major fimbrial subunit was localized and further examined by nucleotide sequence analysis. compa ... | 1988 | 2456966 |