Publications

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lectin purification from carp roe (cyprinus carpio l.), investigation of its carbohydrate specificity and application in histochemistry.a method of lectin purification from carp roe (ccra) was elaborated, which includes affinity chromatography on cross-linked ovomucoid and copolymers of polyvinyl alcohol and blood group-specific substances. that allowed obtaining lectin with electrophoretic purity and yielding of ˜ 42 mg÷kg roe. electrophoresis in 15% polyacrylamide gel in the presence of β-mercaptoethanol showed one band with molecular mass ˜ 15 kda, whereas in the absence of β-mercaptoethanol, ccra exposed band with molecular ...201628002514
a novel igf3 gene in common carp (cyprinus carpio): evidence for its role in regulating gonadal development.since the insulin-like growth factor 3 (igf3) gene was recently discovered in fish ovary, its function in the gonads has received much attention. in this study, we isolated two igf3 subtypes from common carp (cyprinus carpio), which comprised full-length cdna of 707 and 1153 nucleotides encoding 205 and 198 amino acids (aa), respectively. the igf3 aa sequence had the highest gene homology of 72% with the corresponding sequence in zebrafish (danio rerio). phylogenetic tree construction revealed t ...201628002497
short-term effects of t-2 toxin or deoxynivalenol on lipid peroxidation and the glutathione system in common carp.the purpose of this study was to investigate the short-term effects of a single oral dose of t-2 and ht-2 toxin at 0.15, 0.33 and 1.82 mg kg(-1) body weight, or deoxynivalenol (don) and 15-acetyl-don at 0.13, 0.31 and 1.75 mg kg(-1) body weight in common carp. conjugated dienes and trienes (the early markers of lipid peroxidation) were elevated in all don-treated groups at the 16th hour, while thiobarbituric acid reactive substances (tbars; termination marker) were increased at the highest dose ...201627993104
erratum to: prevalence, sensitivity and specificity of antibodies against carbamylated proteins in a monocentric cohort of patients with rheumatoid arthritis and other autoimmune rheumatic diseases.anti-carp antibodies were detected in 117 patients with ra (37.9%), acpa in 190 patients (61.4%) and rf in 202 patients (65.3%). two (2.04%) of the nhs were positive for anti-carp, one nhs (1.02%) was positive for acpa and three nhs were positive for rf (3.06%). among disease controls, anti-carp antibodies were detected in 33 patients (16.5%), acpa in 29 patients (14.5%) and rf in 64 patients (32%). in particular, 16.8% of patients with systemic lupus erythematosus and 31.1% of patients with sjö ...201627986080
new cell motility model observed in parasitic cnidarian sphaerospora molnari (myxozoa:myxosporea) blood stages in fish.cellular motility is essential for microscopic parasites, it is used to reach the host, migrate through tissues, or evade host immune reactions. many cells employ an evolutionary conserved motor protein- actin, to crawl or glide along a substrate. we describe the peculiar movement of sphaerospora molnari, a myxozoan parasite with proliferating blood stages in its host, common carp. myxozoa are highly adapted parasitic cnidarians alternately infecting vertebrates and invertebrates. s. molnari blo ...201627982057
structural, functional and in vitro digestion characteristics of spray dried fish roe powder stabilised with gum arabic.fish roes are considered as nutritionally valuable for their high content of essential fatty acids and amino acids. however, roe lipids undergo considerable extent of oxidation during processing and storage, imparting objectionable bitter taste and rancid flavour to roe products. hence, the objective of the study was to reconstitute the roe mass and microencapsulate lipid fraction, so that small oil droplets are entrapped within a dry matrix of roe proteins during spray drying. prior to spray dr ...201727979150
genome wide identification, phylogeny, and expression of aquaporin genes in common carp (cyprinus carpio).aquaporins (aqps) are integral membrane proteins that facilitate the transport of water and small solutes across cell membranes. among vertebrate species, aqps are highly conserved in both gene structure and amino acid sequence. these proteins are vital for maintaining water homeostasis in living organisms, especially for aquatic animals such as teleost fish. studies on teleost aqps are mainly limited to several model species with diploid genomes. common carp, which has a tetraploidized genome, ...201627935978
application of biotic ligand model in predicting copper acute toxicity to carp (cyprinidae).three representative species of cyprinidae fishes (aristichthys nobilis, ctenopharyngodon idellus, and cyprinus carpio), which are abundant in chinese surface waters, were studied to determine their sensitivity to copper (cu) in acute exposures. we first performed acute toxicity tests to determine the cu lc50 value for each species in water with varying characteristics. the biotic ligand model (blm) was then calibrated using the toxicity data for these species together with binding properties sp ...201727928618
effects of chronic dietary exposure of zinc oxide nanoparticles on the serum protein profile of juvenile common carp (cyprinus carpio l.).zinc oxide (zno) nanoparticles (nps) have been dramatically used in industry, biology, and medicine. despite their interesting physico-chemical properties for application in various industrial, medical, and consumer products, safe use of zno nps are under challenges due to the inadequate information related to their toxicological endpoints. proteomics was applied to evaluate the sub-lethal effects of dietary exposure to zno nps on serum proteome profile of juvenile common carp, (cyprinus carpio) ...201727916310
antibodies against carbamylated proteins and cyclic citrullinated peptides in systemic lupus erythematosus: results from two well-defined european cohorts.articular manifestations are common in systemic lupus erythematosus (sle) whereas erosive disease is not. antibodies to cyclic citrullinated peptide (anti-ccp) are citrulline-dependent in rheumatoid arthritis (ra), whereas the opposite is suggested in sle, as reactivity with cyclic arginine peptide (cap) is typically present. antibodies targeting carbamylated proteins (anti-carp) may occur in anti-ccp/rheumatoid factor (rf)-negative cases long before clinical onset of ra. we analysed these antib ...201627912793
effects of dietary vitamin e deficiency on systematic pathological changes and oxidative stress in fish.the aim of this study was to investigate the effects of dietary vitamin e deficiency on systematic pathological changes and oxidative stress in fish. a total of 320 healthy common carp (cyprinus carpio) were randomized into four groups; the control group was fed a basal diet supplemented with 100 iukg-1 of vitamin e, while the three experimental groups were fed the same basal diet with reduced vitamin e content (0, 25, or 50 iukg-1). findings showed that fish in the experimental groups mainly pr ...201627911874
smoking is associated with the concurrent presence of multiple autoantibodies in rheumatoid arthritis rather than with anti-citrullinated protein antibodies per se: a multicenter cohort study.the contribution of smoking to rheumatoid arthritis (ra) is hypothesized to be mediated through formation of anti-citrullinated protein antibodies (acpa). in ra, however, autoantibodies such as acpa, rheumatoid factor (rf), and anti-carbamylated protein antibodies (anti-carp) often occur together, and it is thus unclear whether smoking is specifically associated with some autoantibodies rather than others. we therefore investigated whether smoking is only associated with acpa or with the presenc ...201627906045
plasticity in growth of farmed and wild atlantic salmon: is the increased growth rate of farmed salmon caused by evolutionary adaptations to the commercial diet?domestication of atlantic salmon for commercial aquaculture has resulted in farmed salmon displaying substantially higher growth rates than wild salmon under farming conditions. in contrast, growth differences between farmed and wild salmon are much smaller when compared in the wild. the mechanisms underlying this contrast between environments remain largely unknown. it is possible that farmed salmon have adapted to the high-energy pellets developed specifically for aquaculture, contributing to ...201627905882
prevalence, sensitivity and specificity of antibodies against carbamylated proteins in a monocentric cohort of patients with rheumatoid arthritis and other autoimmune rheumatic diseases.antibodies against carbamylated proteins (anti-carp) have been recently identified in the sera of patients with rheumatoid arthritis (ra). the objective of the study was to evaluate the prevalence, sensitivity and specificity of anti-carp compared to anti-citrullinated peptide antibodies (acpa) and rheumatoid factor (rf), replicating the existing data in a large cohort of italian patients with ra and extending the evaluation to other autoimmune rheumatic diseases (airds).201627887639
zebrafish embryonic development is induced by carp sperm.haploid gynogenetic screens increase the efficiency of forward genetic screens and linkage analysis in fish. typically, uv-irradiated zebrafish sperm is used to activate zebrafish oocytes for haploid screens. we describe the use of uv-irradiated common carp sperm to activate haploid gynogenetic zebrafish development. carp × zebrafish hybrids are shown to have a characteristic set of features during embryonic development and exhibit functional development of several tissues (muscle, heart and ner ...201627881764
parasitic infection alters haematology and immunity parameters of common carp, cyprinus carpio, linnaeus, 1758.the present study was conducted to investigate the effects of parasite infection on haematological parameters of common carp, cyprinus carpio. to this end, fish with same age were sampled from a warm water fish farm. after fish sampling, fish were transferred to lab for parasite study. parasite incidence percent and density were calculated for two population carps. after parasite study, to investigate the haematological parameters, the blood sampling was carried out from two group fish i.e. infe ...201627876978
alternations in the liver enzymatic activity of common carp, cyprinus carpio in response to parasites, dactylogyrus spp. and gyrodactylus spp.the present study was carried out to investigate the effects of parasites, monogenea, dactylogyrus spp. and gyrodactylus spp. on some enzymatic and biochemical components of liver in healthy and infected common carp, cyprinus carpio. for this purpose, 10 healthy and 10 infected fish were collected from farm. the blood samples were taken and after separation of serum, the values of aspartate aminotransferase (ast), alanine aminotransferase (alt) enzymes activities as well as creatinine and urea w ...201627876903
blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy.fish is a frequent elicitor of severe ige-mediated allergic reactions. beside avoidance, there is currently no allergen-specific therapy available. hypoallergenic variants of the major fish allergen, parvalbumin, for specific immunotherapy based on mutation of the 2 calcium-binding sites have been developed.201627876628
morphological features of an endangered japanese strain of cyprinus carpio: reconstruction based on seven snp markers.morphological analyses of 183 specimens of japanese common carp cyprinus carpio (171 from lake biwa and 12 from nursery ponds) using genetic hybrid indices demonstrated that the typical native japanese strain of c. carpio has a more elongate body, more branched dorsal-fin rays, fewer and shorter gill rakers, more developed pneumatic bulb, more coiled pneumatic duct, longer posterior swimbladder and shorter intestine than the typical introduced c. carpio. these results provide a basis for a bette ...201727859297
european carp (cyprinus carpio l.) protein-derived ex vivo digests and in vitro hydrolysates differ in the ace i inhibitory activity and composition of released ace inhibitory peptides.carp muscle tissue is a valuable source of biologically active constituents known to positively influence human health. in this study, carp protein digests/hydrolysates generated by human/ porcine digestive enzymes were analysed for their angiotensin i-converting enzyme inhibitory (acei) activity. the ex vivo digests and in vitro hydrolysates were used in a screening for acei peptides as well. carp proteins were hydrolysed more rapidly by human gastrointestinal juices than by porcine enzymes. sa ...201727855606
description of a myxozoan parasite myxobolus sp.n pkb2014 from an edible fish, with emphasis on its molecular characterization.in india, more than 104 species of myxobolus have so far been reported infecting freshwater as well as marine fishes. the study focuses on the description of a new myxosporean species, myxobolus sp.n pkb 2014 from the gill lamellae of an indian major carp labeo rohita. the species have been described on the basis of morphological characterization of the spores, tissue architecture and 18s rdna sequence data. the plasmodia of myxobolus sp.n pkb 2014 were round in shape measuring, 50 to 70 μm in d ...201527844012
description and experimental transmission of tetracapsuloides vermiformis n. sp. (cnidaria: myxozoa) and guidelines for describing malacosporean species including reinstatement of buddenbrockia bryozoides n. comb. (syn. tetracapsula bryozoides).this paper provides the first detailed description of a tetracapsuloides species, tetracapsuloides vermiformis n. sp., with vermiform stages in the bryozoan host, fredericella sultana, and its experimental transmission from f. sultana to cyprinus carpio. the suitability of morphological, biological and 18s rdna sequence data for discrimination between malacosporean species is reviewed and recommendations are given for future descriptions. presently, malacosporean species cannot be differentiated ...201727842616
effect of t-2 toxin-contaminated diet on common carp (cyprinus carpio l.).the t-2 toxin, a fungal metabolite produced by fusarium molds, occurs in a range of agriculture products. reduced availability of fish meal has led to increasing use of cereals as a source of protein in commercial aquaculture feeds, which has increased the potential for mycotoxin contamination. the purpose of this study was to investigate toxicity of t-2 toxin intake in common carp (cyprinus carpio l.) using haematological, biochemical and immunological parameters and oxidative stress indices. i ...201727840172
the evolution, structure and function of the ray finned fish (actinopterygii) glucocorticoid receptors.basal ray-finned fish (actinopterygii) possess a single glucocorticoid receptor (gr) and when compared to the lobe-finned vertebrate (sarcopterygii) gr possess nine additional amino acids between the zinc-finger of the dna binding domain. a whole genome duplication event which occurred between 320 and 350mya in the teleost lineage following the split from the basal ray-finned fish resulted in 2 grs: one gr group, gr1, has retained the 9 amino acids insert whereas the other group, gr2, has not. t ...201627838382
quantitative trait loci for morphometric traits in multiple families of common carp (cyprinus carpio).quantitative trait locus (qtl) mapping is frequently used to understand the genetic architecture of quantitative traits. herein, we performed a genome scan for qtl affecting the morphometric characters in eight full-sib families containing 522 individuals using different statistical methods (sib-pair and half-sib model). a total of 194 qtls were detected in 25 different regions on 10 linkage groups (lgs). among them, 37 qtls on five lgs (eight, 13, 24, 40 and 45) were significant (5% genome-wide ...201727826895
poly i:c facilitates the phosphorylation of ctenopharyngodon idellus type i ifn receptor subunits and jak kinase.members of the janus kinase (jak) family, jak1 and tyk2 take part in jak-stat signaling pathway mediated by interferon in mammalian cells. similar to the mammalian counterparts, fish jak1 and tyk2 also perform their potential biological activities by phosphorylating cytokine receptors and stat. in the present study, ctenopharyngodon idellus jak1 (cijak1) and tyk2 (cityk2) were cloned and identified. the full-length cdna of cijak1 (kt724352.1) is 3829 bp, with an open reading frame (orf) of 3465 ...201727815207
effects of ndrg1 family proteins on photoreceptor outer segment morphology in zebrafish.rods and cones are functionally and morphologically distinct. we previously identified n-myc downstream-regulated gene 1b (ndrg1b) in carp as a cone-specific gene. here, we show that ndrg1b and its paralog, ndrg1a-1, contribute to photoreceptor outer segment (os) formation in zebrafish. in adult zebrafish photoreceptors, ndrg1a-1 was localized in the entire cone plasma membranes, and also in rod plasma membranes except its os. ndrg1b was expressed specifically in cones in the entire plasma membr ...201627811999
dietary phytoimmunostimulant persian hogweed (heracleum persicum) has more remarkable impacts on skin mucus than on serum in common carp (cyprinus carpio).immunostimulation through the use of sustainable and eco-friendly dietary additives is one of the current prophylactic strategies in fish husbandry. plant-based immunostimulants are highly considered for this intent, both for their scientific and practical advantages. persian hogweed (heracleum persicum) is a flower-bearing herb that possesses interesting pharmacological importance due to its bioactive compounds. it is commonly used as spice, food additive, dietary supplement and traditional rem ...201627751916
cardiac ankyrin repeat protein attenuates cardiomyocyte apoptosis by upregulation of bcl-2 expression.cardiac ankyrin repeat protein (carp) is a nuclear transcriptional co-factor that has additional functions in the myoplasm as a component of the muscle sarcomere. previous studies have demonstrated increased expression of carp in cardiovascular diseases, however, its role in cardiomyocyte apoptosis is unclear and controversial. in the present study, we investigated possible roles of carp in hypoxia/reoxygenation (h/r) -induced cardiomyocyte apoptosis and the underlying mechanisms. neonatal mouse ...201627713078
antioxidant balance and accumulation of advanced glycation end products after the consumption of standard diets including maillard reaction products from silver carp peptides.the aim of this research was to investigate the accumulation and excretion of ages including fluorescent compounds and n-ε-carboxymethyllysine (cml) in streptozotocin-induced diabetes c57bl/6j mice fed on a diet containing maillard reaction products (mrps) from a silver carp peptide-glucose model system at different heating temperatures for the first time, and analyze the relationship between their in vitro antioxidant activity based on dpph and orac assays and in vivo oxidative stress. the in v ...201627805212
the roles of tissue nitrate reductase activity and myoglobin in securing nitric oxide availability in deeply hypoxic crucian carp.in mammals, treatment with low doses of nitrite has a cytoprotective effect in ischemia/reperfusion events, as a result of nitric oxide formation and s-nitrosation of proteins. interestingly, anoxia-tolerant lower vertebrates possess an intrinsic ability to increase intracellular nitrite concentration during anoxia in tissues with high myoglobin and mitochondria content, such as the heart. here, we tested the hypothesis that red and white skeletal muscles develop different nitrite levels in cruc ...201627742892
facilitated bioaccumulation of perfluorooctanesulfonate in common carp (cyprinus carpio) by graphene oxide and remission mechanism of fulvic acid.as one of the most popular carbon-based nanomaterials, graphene oxide (go) has the potential to be released in aquatic environment and interact with some coexistent organic pollutants, such as perfluorooctanesulfonate (pfos), which is an emerging persistent organic pollutant. in this study, the adsorption of pfos on go in the presence of fulvic acid (fa), the impacts of go and fa on pfos toxicokinetics in carp (cyprinus carpio), and in vitro digestion behaviors were examined. the results indicat ...201627718559
a light-colored region of caudal fin: a niche of melanocyte progenitors in crucian carp (cyprinus carpio l.).melanocyte stem cells are a population of immature cells which sustain the self-renewal and replenish the differentiated melanocytes. in this research, a light-colored region (lcr) is observed at the heel of caudal fin in juvenile crucian carp. by cutting off the caudal fin, the operated caudal fin can regenerate in accordance with the original pigment pattern from the retained lcr. as markers of stem cells, oct4 and sox2 have been found to be highly expressed in the lcr as well as mitfa, a labe ...201727797132
complexity of gap junctions between horizontal cells of the carp retina.in the vertebrate retina, horizontal cells (hcs) reveal homologous coupling by gap junctions (gj), which are thought to consist of different connexins (cx). however, recent studies in mouse, rabbit and zebrafish retina indicate that individual hcs express more than one connexin. to provide further insights into the composition of gj connecting hcs and to determine whether hcs express multiple connexins, we examined the molecular identity and distribution of gj between hcs of the carp retina. we ...201727793781
molecular characterization of activin receptor type iia and its expression during gonadal maturation and growth stages in rohu carp.activin receptor type iia (actriia), a transmembrane serine/threonine kinase receptor is an important regulator of physiological traits, viz., reproduction and body growth in vertebrates including teleosts. however, existing knowledge of its role in regulating fish physiology is limited. to address this, we have cloned and characterized the actriia cdna of labeo rohita (rohu), an economically important fish species of the indian subcontinent. comparative expression profiling of the receptor gene ...201727575753
pre-acclimation to low ammonia improves ammonia handling in common carp (cyprinus carpio) when exposed subsequently to high environmental ammonia.we tested whether exposing fish to low ammonia concentrations induced acclimation processes and helped fish to tolerate subsequent (sub)lethal ammonia exposure by activating ammonia excretory pathways. common carp (cyprinus carpio) were pre-exposed to 0.27mm ammonia (∼10% 96h lc50) for 3, 7 and 14days. thereafter, each of these pre-exposed and parallel naïve groups were exposed to 1.35mm high environmental ammonia (hea, ∼50% 96h lc50) for 12h and 48h to assess the occurrence of ammonia acclimati ...201627788451
nanocerium oxide increases the survival of adult rod and cone photoreceptor in culture by abrogating hydrogen peroxide-induced oxidative stress.in vitro cell culture system for adult rod and cone photoreceptor (pr) is an effective and economical model for screening drug candidates against all kinds of age related retinal blindness. interestingly, adult pr cells have a limited survival in the culture system, thus preventing full exploitation of this in vitro approach for drug screening applications. the limited survival of the adult pr cells in culture is due to their inherently high oxidative stress and photic injury. mixed valence-stat ...201627782496
copper bioaccumulation and depuration in common carp (cyprinus carpio) following co-exposure to tio2 and cuo nanoparticles.metal oxide nanoparticles (nps), such as tio2 and cuo, are widely applied in an increasing number of products and applications, and therefore their release to the aquatic ecosystems is unavoidable. however, little is known about joint toxicity of different nps on tissues of aquatic organisms, such as fish. this study was conducted to assess the uptake and depuration of cu following exposure to cuo nps in the presence of tio2 nps in the liver, intestine, muscle, and gill of common carp, cyprinus ...201627638716
dietary choline deficiency and excess induced intestinal inflammation and alteration of intestinal tight junction protein transcription potentially by modulating nf-κb, stat and p38 mapk signaling molecules in juvenile jian carp.this study investigated the effects of choline on intestinal mucosal immune and the possible mechanisms in fish by feeding juvenile jian carp (cyprinus carpio var. jian) with graded levels of dietary choline (165-1820 mg/kg diet) for 65 days. the results firstly showed that choline deficiency induced inflammatory infiltration in the proximal intestine (pi), mid intestine (mi) and distal intestine (di) of fish. meanwhile, compared with the optimal choline group, choline deficiency decreased the a ...201627693201
agaricus bisporus powder improved cutaneous mucosal and serum immune parameters and up-regulated intestinal cytokines gene expression in common carp (cyprinus carpio) fingerlings.the aim of the present study was to investigate immunomodulatory effects of agaricus bisporus, white bottom mushroom powder (wbmp) on common carp (cyprinus carpio) fingerlings. carps were fed on different levels of wbmp (0, 0.5, 1 and 2%) for 8 weeks and at the end of feeding trial, skin mucus immune parameters (total ig, lysozyme and protease activity), cytokines gene expression (tnf-alpha, il1b, il8) in intestine as well as serum non-specific immune parameters (total ig, lysozyme and ach50) we ...201627678509
protective effects of calcium on cadmium accumulation in co-cultured silver carp (hypophthalmichthys molitrix) and triangle sail mussel (hyriopsis cumingii).discovering cost effective strategies to reduce cadmium (cd) uptake is of great concern for consumer food safety in the aquaculture industry. this study investigated the protective effects of calcium (ca) on cd uptake in co-cultured silver carp (hypophthalmichthys molitrix) and triangle sail mussel (hyriopsis cumingii). the results show that ca-depending on its applied concentration-caused a significant decrease in the cd uptake into muscle (by 48 %-72 %), gills (by 51 %-57 %), liver (by 52 %-81 ...201627761596
sublethal toxicity of quinalphos on oxidative stress and antioxidant responses in a freshwater fish cyprinus carpio.extensive use of quinalphos, an organophosphorus pesticide, is likely to reach the aquatic environment and thereby posing a health concern for aquatic organisms. oxidative stress and antioxidant responses may be good indicators of pesticide contamination in aquatic organisms. the data on quinalphos induced oxidative stress and antioxidant responses in carps are scanty. this study is aimed to assess the two sublethal concentrations of quinalphos (1.09 and 2.18 μl l(-1) ) on oxidative stress and a ...201625899319
telemetry-determined habitat use informs multi-species habitat management in an urban harbour.widespread human development has led to impairment of freshwater coastal wetlands and embayments, which provide critical and unique habitat for many freshwater fish species. this is particularly evident in the laurentian great lakes, where such habitats have been severely altered over the last century as a result of industrial activities, urbanization, dredging and infilling. in toronto harbour, extensive restoration efforts have been directed towards improving the amount and quality of aquatic ...201727744518
toxicological hazard induced by sucralose to environmentally relevant concentrations in common carp (cyprinus carpio).sucralose (suc) is an artificial sweetener that is now widely used in north american and europe; it has been detected in a wide variety of aquatic environments. it is considered safe for human consumption but its effects in the ecosystem have not yet been studied in depth, since limited ecotoxicological data are available in the peer-reviewed literature. this study aimed to evaluate potential suc-induced toxicological hazard in the blood, brain, gill, liver and muscle of cyprinus carpio using ox ...201727744200
transcriptome analysis reveals that insulin is an immunomodulatory hormone in common carp.common carp (cyprinus carpio) is a widespread freshwater fish and economically important species in china and other east asian countries. recent studies suggest that insulin can alter the expression of immune genes and, thus, can be regarded as an immunomodulatory hormone. to understand the mechanism of the immune response to insulin, we performed a comparative rna-seq transcriptome analysis using livers from common carp injected with insulin (5 μg/g bodyweight) or saline as a control. after fil ...201627742590
aquatic nuisance species in the great lakes and mississippi river basin-a risk assessment in support of glmris.the u.s. army corps of engineers is conducting the great lakes and mississippi river interbasin study to identify the highest risk aquatic nuisance species currently established in either the mississippi river basin or the great lakes basin and prevent their movement into a new basin. the great lakes and mississippi river interbasin study focuses specifically on aquatic nuisance species movement through the chicago area waterway system, a multi-use waterway connecting the two basins. in support ...201727734087
the c-terminal dimerization motif of cyclase-associated protein is essential for actin monomer regulation.cyclase-associated protein (cap) is a conserved actin-regulatory protein that functions together with actin depolymerizing factor (adf)/cofilin to enhance actin filament dynamics. cap has multiple functional domains, and the function to regulate actin monomers is carried out by its c-terminal half containing a wiskott-aldrich syndrome protein homology 2 (wh2) domain, a cap and x-linked retinitis pigmentosa 2 (carp) domain, and a dimerization motif. wh2 and carp are implicated in binding to actin ...201627729544
geno- and cytotoxicity induced on cyprinus carpio by aluminum, iron, mercury and mixture thereof.metals such as al, fe and hg are used in diverse anthropogenic activities. their presence in water bodies is due mainly to domestic, agricultural and industrial wastewater discharges and constitutes a hazard for the organisms inhabiting these environments. the present study aimed to evaluate geno- and cytotoxicity induced by al, fe, hg and the mixture of these metals on blood of the common carp cyprinus carpio. specimens were exposed to the permissible limits in water for human use and consumpti ...201727721126
podophyllum hexandrum ameliorates endosulfan-induced genotoxicity and mutagenicity in freshwater cyprinid fish crucian carp.medicinal plants continue to act as a repository for novel drug leads with novel mechanisms of action. podophyllum hexandrum royale (berberideceae) treats diverse conditions in folk medicine.201727718769
consistent individual differences in seed disperser quality in a seed-eating fish.animal-mediated seed dispersal (zoochory) is considered to be an important mechanism regulating biological processes at larger spatial scales. to date, intra-specific variation in seed disperser quality within seed-dispersing animals has not been studied. here, i employed seed feeding trials to quantify individual differences in disperser quality within the common carp (cyprinus carpio) using seeds of two aquatic plants: unbranched bur-reed (sparganium emersum, sparganiaceae) and arrowhead (sagi ...201727704241
transfer, tissue distribution and bioaccumulation of microcystin lr in the phytoplanktivorous and carnivorous fish in anzali wetland, with potential health risks to humans.this study was conducted to determine microcystin lr concentration in the aquatic ecosystem of the anzali wetland in iran. extraction and detection of mc-lr from the water and fish samples was conducted by the spe and hplc-uv apparatus. our results showed that among toxic cyanobacteria, anabaena was the dominant genera during the study period. the results indicated that mc-lr content in water ranged from 0.18 to 3.02μg/l in dissolved fraction and undetectable level to 1.13μg/l in cellular fracti ...201727697341
structural and functional characterization of recombinant interleukin-10 from indian major carp labeo rohita.interleukin-10, an important regulator of both the innate and adaptive immune systems, is a multifunctional major cytokine. though it is one of the major cytokines, il-10 from the indian major carp, labeo rohita, has not yet been characterized. in the present study, we report large scale production and purification of biologically active recombinant il-10 of l. rohita (rlril-10) using a heterologous expression system and its biophysical and functional characterization. high yield (~70 mg/l) of s ...201727689097
canthaxanthin: from molecule to function.the aim of this review is to summarize the relevant literature about the use of canthaxanthin in food science and nutrition research. canthaxanthin is a red-orange carotenoid that belongs to the xanthophyll group. this naturally occurring pigment is present in bacteria, algae and some fungi. canthaxanthin is also responsible for the color of flamingo feathers, koi carp skin and crustacean shells. canthaxanthin is widely used in poultry (broiler, laying hens) as a feed additive. canthaxanthin can ...201627687695
carp-1 functional mimetics are novel inhibitors of drug-resistant triple negative breast cancers.doxorubicin and cisplatin are the frontline therapeutics for treatment of the triple negative breast cancers (tnbcs). emergence of drug-resistance often contributes to failure of drugs and poor prognosis, and thus necessitates development of new and improved modalities to treat tnbcs. we generated and characterized chemotherapy-resistant tnbc cells following their culture in chronic presence of doxorubicin or cisplatin, and tested whether their viabilities were inhibited by a novel class of carp ...201627687593
accumulation of metals in juvenile carp (cyprinus carpio) exposed to sublethal levels of iron and manganese: survival, body weight and tissue.many oxbows are contaminated by fe and mn as a consequence of the elemental concentration of sediment and water originating from the upper tisza region of hungary. the phenomenon is partly caused by anthropogenic activities and mainly due to the geochemical characteristics of the region. the effects of fe and mn on the aquatic ecosystem of these wetlands were investigated in a model experiments in this study. survival, individual body weight and the elemental concentrations of organs were determ ...201727683024
assessment of heavy metal pollution risks in yonki reservoir environmental matrices affected by gold mining activity.this study reports the heavy metal (hg, cd, cr, cu, and pb) contamination risks to and safety of two species of fresh water fish (tilapia, oreochromis mossambicus and carp, cyprinus carpio) that are farmed in the yonki reservoir in the eastern highlands of papua new guinea (png). the upper reaches of the reservoir are affected by alluvial and large-scale gold mining activities. we also assessed heavy metal levels in the surface waters and sediments and in selected aquatic plant species from the ...201627663878
genetic distances of crucian carp populations analyzed by pcr approach.genomic dnas isolated from crucian carp of four rivers, belonging to the family cyprinidae was amplified by seven oligonucleotides primers. in the present study, we employed hierarchical clustering method in order to reveal genetic distances and variations. crucian carp was acquired from hangang river (cah), geumgang river (cag), nakdonggang river (can) and yeongsangang river (cay). the primer bion-12 generated the most loci (a total of 50) with an average of 10 in the cay population. the primer ...201627660831
effect of single and combination of three triazine metabolites at environmental concentrations on early life stages of common carp (cyprinus carpio l.).the sensitivity of early life stages of common carp (cyprinus carpio l.) to chronic exposure to single and combined environmental concentrations of the triazine metabolites terbuthylazine 2-hydroxy, terbuthylazine-desethyl and atrazine 2-hydroxy was evaluated under laboratory conditions. their effects were assessed on lipid peroxidation, antioxidant enzymes (total superoxide dismutase, glutathione reductase, catalase, glutathione s-transferase, reduced glutathione), mortality, growth, developmen ...201627650852
histopathological effects following short-term coexposure of cyprinus carpio to nanoparticles of tio2 and cuo.the aim of this research was to investigate the coexposure of nanoparticles of titanium dioxide (tio2) and copper oxide (cuo) on the alterations of the gill, intestine, kidney, and liver tissues of carps (cyprinus carpio). in this study, carps (length 23 ± 1.5 cm; weight 13 ± 1.3 g) were divided into six groups of 15 each and exposed to 2.5 and 5.0 mg l(-1) of cuo nanoparticles (nps), 10.0 mg l(-1) of tio2 nps, and 2.5 and 5.0 mg l(-1) of cuo nps + 10.0 mg l(-1) of tio2 np mixture. fish were sam ...201627650436
molecular characterization and growth association of two apolipoprotein a-ib genes in common carp (cyprinus carpio).apolipoprotein a-i (apoa-i) is functionally involved in the transportation and metabolism of lipids in vertebrates. in this study, two isoforms of apoa-ib in common carp (cyprinus carpio l.) were characterized. sequence comparison and phylogenetic analysis showed that c. carpio apoa-ib is relatively conserved within cyprinid fishes. during embryonic development, c. carpio apoa-ib was first expressed at the stage of multi-cells, and the highest mrna level was observed at the stage of optic vesicl ...201627649163
protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss).the purpose of the study was to analyze seminal plasma composition, sperm production, and sperm motility over the course of the spawning season in the common carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss). the highest mean percent of motile sperm (carp, 98.3 ± 1.6%; rainbow trout, 92.8 ± 5.6%) and highest spermatozoon velocity (carp, 286.3 ± 7.8 μm sec(-1) ; rainbow trout, 245.3 ± 8.3 μm sec(-1) ) were observed in the middle phase of the spawning period, at 5 sec post-activation. ...201627641056
characterization of sections of the sava river based on fish community structure.sampling was undertaken, with the same fishing gear and along the sava river, from its source to its confluence, in september 2014 and september 2015. in total, 44 fish species were identified, of which 37 were native species and 7 were alien. fish samples revealed independence in terms of both species composition and their abundance under different hydrological conditions. during flooding and high water levels in 2014, pelagic fish species were sampled in greater proportion than at lower water ...201727639023
identification of small peptides of acidic collagen extracts from silver carp skin and their therapeutic relevance.low-temperature techniques that prevent protein denaturation are being used to extract collagen from fish skin for cosmetic purposes. these extracts contain collagen with its triple helix structure preserved, as well as a number of other proteins.201627627554
aphanius sophiae (actinoptrygii, cyprinodontidae), a new host for ichthyophthirius multifiliis (ciliophora) reported from iran.aphanius sophiae (heckel, 1849) or soffia tooth-carp is endemic species to the endorheic kor river basin, fars province, southwest of iran. in spite of importance of this species as a biologic control of anopheles larvae, little attention has been paid to its diseases particularly parasitic infections. a total of 24 a. sophiae (12 males and 12 females) in safashahr spring, kor river basin, were collected by using dip net in february 2012. surface of all fish were gross examined and screened for ...201627605832
nkx2.5/carp signaling pathway contributes to the regulation of ion channel remodeling induced by rapid pacing in rat atrial myocytes.remodeling of atrial electrophysiology and structure is the primary feature of atrial fibrillation (af). evidence suggests that abnormalities in the expression levels of embryological cardiovascular development‑associated transcription factors, including nkx2.5, are crucial for the development of af. rat atrial myocardial cells (amcs) in culture dishes were placed in an electric field and stimulated. transmission electron microscopy was used to observe the ultrastucture prior to and following ra ...201627600472
the impact of stunning methods on stress conditions and quality of silver carp (hypophthalmichthys molitrix) fillets stored at 4°c during 72h postmortem.this study aimed to evaluate different stunning methods [percussion (t1), immersion in ice/water slurry (t2), and gill cut (t3)] on quality and stress conditions of silver carp (hypophthalmichthys molitrix) fillets stored at 4°c in 72h postmortem. rigor index (ri%), behavioral analysis, levels of lactic acid and muscle glycogen were measured for stress level evaluation. meanwhile, sensory assessment, texture properties, cooking loss, adenosine triphosphate (atp) related compounds, adenosine mono ...201727596401
exposure to sub-acute doses of fipronil and buprofezin in combination or alone induces biochemical, hematological, histopathological and genotoxic damage in common carp (cyprinus carpio l.).use of pesticides or insecticides can be highly toxic to aquatic life forms due to leaching and agricultural runoff, rains or flood. fipronil (fp) is a gaba receptor inhibitor, while buprofezin (bpfn) is an insect growth regulator. presently, we exposed groups of aquaria acclimated carp fish (cyprinus carpio) for 96h to sub-lethal concentrations of fipronil (400μgl(-1); 9.15×10(-7)moll(-1)) and buprofezin (bpfn, 100mgl(-1); 1.072×10(-6)moll(-1)) singly or in combination. the extent of damage was ...201627595653
a full-body transcriptome and proteome resource for the european common carp.the common carp (cyprinus carpio) is the oldest, most domesticated and one of the most cultured fish species for food consumption. besides its economic importance, the common carp is also highly suitable for comparative physiological and disease studies in combination with the animal model zebrafish (danio rerio). they are genetically closely related but offer complementary benefits for fundamental research, with the large body mass of common carp presenting possibilities for obtaining sufficien ...201627590662
carp il10a and il10b exert identical biological activities in vitro, but are differentially regulated in vivo.we recently reported on the functional characterization of carp il10. we showed that carp il10 is able to downregulate proinflammatory activities by carp phagocytes and promote b cell proliferation, differentiation and antibody production as well as proliferation of memory t cells. taking advantage of the recent annotation of the carp genome, we completed the sequence of a second il10 paralogue, named il10b, the presence of which was expected owing to the recent (8 million years ago) fourth roun ...201727586813
lipid composition in common carp (cyprinus carpio) sperm possessing different cryoresistance.the present study examined the lipid composition of plasma membranes in carp sperm with different post-thaw motility. the approach adapted for carp sperm cryopreservation, which involves the selection of the most effective protocol for individual males by comparing two cryoprotective media, was applied to the cryopreservation procedure. sperm motility prior to freezing was greater than 80% but decreased to 40% in one group and to 10% in another group following cryopreservation. lipid content of ...201627574978
improvement of common carp (cyprinus carpio) sperm cryopreservation using a programable freezer.the applicability of a programmable freezer for the increased-scale cryopreservation of common carp sperm was investigated. the effect of different equilibration times, cryopreservation methods, extenders, dilution ratios, activating solutions on the post-thaw motility of common carp sperm was investigated. the suitable post-thaw storage time-interval as well as fertilizing capacity of cryopreserved sperm was also examined. the motility, curvilinear velocity (vcl) and straightness (str) values d ...201627570061
infection and immunity against ichthyophthirius multifiliis in zebrafish (danio rerio).ichthyophthirius multifiliis, causing white spot disease, is a serious pathogen in aquaculture as well as for the ornamental fish industry. in carp, channel catfish and rainbow trout the immune responses against the parasite have been partly elucidated and these species are able to acquire a high level of immunity against the disease. zebrafish are however, known to be more resilient towards the disease than channel catfish but the pathology and the ability to obtain protection is unknown. in th ...201627567935
impacts of loach bioturbation on the selective bioaccumulation of hbcdd diastereoisomers and enantiomers by mirror carp in a microcosm.to assess the impacts of bioturbation at the water-sediment interface on the bioaccumulation of hexabromocyclododecane diastereoisomers (hbcdds) by pelagic organisms and the bioisomerization and enantioselectivity therein, we built microcosms containing water, mirror carp (cyprinus carpio), and sediment. the microcosms were sorted into two groups, with or without loach (misgurnus anguillicaudatus) living at the water-sediment interface. a 50-d accumulation test was conducted by spiking the micro ...201627565315
dimethoate induced behavioural changes in juveniles of cyprinus carpio var. communis under temperate conditions of kashmir, india.the present study was designed to investigate acute toxicity of dimethoate on juvenile cyprinus carpio var. communis. fishes weighing 10 ± 2 gms were selected and mortality data was statistically evaluated by finney's probit method. the 96-hour lc50 value for cyprinus carpio was found as 1.1 ppm in static bioassay system. mean values of physicochemical parameters of aquarium waters determined during bioassay depicted slight variation indicating that the mortality in aquarium fishes occurred due ...201627563689
how could fully scaled carps appear in natural waters in madagascar?the capacity of organisms to rapidly evolve in response to environmental changes is a key feature of evolution, and studying mutation compensation is a way to evaluate whether alternative routes of evolution are possible or not. common carps (cyprinus carpio) carrying a homozygous loss-of-function mutation for the scale cover gene fgfr1a1, causing the 'mirror' reduced scale cover, were introduced in madagascar a century ago. here we show that carps in malagasy natural waters are now predominantl ...201627559059
toxic effects of copper sulfate and copper nanoparticles on minerals, enzymes, thyroid hormones and protein fractions of plasma and histopathology in common carp cyprinus carpio.differences in toxicological effects of dissolved copper and copper nanoparticles were studied in common carp, cyprinus carpio. the fish were exposed to 0.25mgl(-1) copper as copper sulfate (0.25cu), 0.25mgl(-1) copper as copper oxide nanoparticles (0.25ncu) and 25mgl(-1) copper as copper oxide nanoparticles (25ncu) over 14days. plasma biochemical, enzymatic and hormonal characteristics, and liver and kidney histopathology were examined at the end of the experiment. the results showed that both ...201627555376
anti-carbamylated protein antibodies as a reproducible independent type of rheumatoid arthritis autoantibodies.a large fraction of the patients with rheumatoid arthritis (ra) develop specific autoantibodies, which until recently were only of two types, rheumatoid factor (rf) and anti-citrullinated protein antibodies (acpa). we aimed to replicate important findings about a recently described third type of specific autoantibodies, anti-carbamylated protein (anti-carp) antibodies, because they have been described based only in the homemade elisa from a single laboratory. our study included 520 patients with ...201627537849
carp interacts with titin at a unique helical n2a sequence and at the domain ig81 to form a structured complex.the cardiac ankyrin repeat protein (carp) is up-regulated in the myocardium during cardiovascular disease and in response to mechanical or toxic stress. stress-induced carp interacts with the n2a spring region of the titin filament to modulate muscle compliance. we characterize the interaction between carp and titin-n2a and show that the binding site in titin spans the dual domain un2a-ig81. we find that the unique sequence un2a is not structurally disordered, but that it has a stable, elongated ...201627531639
determination of eugenol in fish and shrimp muscle tissue by stable isotope dilution assay and solid-phase extraction coupled gas chromatography-triple quadrupole mass spectrometry.in this study, we developed a new method for the accurate quantification of eugenol in fish samples based on stable isotope dilution assay (sida) and solid-phase extraction (spe) coupled gas chromatography-triple quadrupole mass spectrometry (sida-spe-gc-ms/ms). due to the difference of matrix effect (me), it was difficult to determine accurately the level of eugenol residue in different fish and shrimp samples based on external standard calibration method. sida was applied to compensate matrix ...201627531030
cell fusion as the formation mechanism of unreduced gametes in the gynogenetic diploid hybrid fish.the gynogenetic diploid hybrid clone line (gdh) derived from red crucian carp (♀ rcc) × common carp (♂ cc) possesses the unusual reproductive trait of producing unreduced diploid eggs. to identify the mechanism underlying this phenomenon, we examined the structure, in vivo developmental process and in vitro dynamic development of the gdh gonad. in summary, compared with rcc and cc, gdh showed certain special straits. first, a high frequency (84.7%) of germ cell fusion occurred in gonadal tissue ...201627530321
association study between single nucleotide polymorphisms in leptin and growth traits in cyprinus carpio var. jian.leptin is a hormone that affects the regulation of body weight, energy expenditure, fat metabolism, food intake, and appetite. in this study, we cloned the jllep-a1 and jllep-a2 genes in jian carp (cyprinus carpio var. jian) and performed an association analysis between identified polymorphisms and growth traits. three polymorphisms in exons of jllep-a1 (a1-t113c) and jllep-a2 (a2-g415a and a2-g427a) were identified, and genotyped by the polymerase chain reaction - restriction fragment length po ...201627525905
reference gene validation for quantification of gene expression during final oocyte maturation induced by diethylstilbestrol and di-(2-ethylhexyl)-phthalate in common carp.final oocyte maturation is the key step to successful spawning and fertilization. quantitative real-time pcr (qpcr) is the technique of election to quantify the abundance of functional genes in such study. reference gene is essential for correct interpretation of qpcr data. however, an ideal universal reference gene that is stable under all experimental circumstances has not been described. researchers should validate their reference genes while performing qpcr analysis. the expression of 6 cand ...201627521935
environment shapes the fecal microbiome of invasive carp species.although the common, silver, and bighead carps are native and sparsely distributed in eurasia, these fish have become abundant and invasive in north america. an understanding of the biology of these species may provide insights into sustainable control methods. the animal-associated microbiome plays an important role in host health. characterization of the carp microbiome and the factors that affect its composition is an important step toward understanding the biology and interrelationships betw ...201627514729
a scalable approach to capacitated arc routing problems based on hierarchical decomposition.the capacitated arc routing problem (carp) is a challenging optimization problem with lots of applications in the real world. numerous approaches have been proposed to tackle this problem. most of these methods, albeit showing good performance on carp instances of small and median sizes, do not scale well to large-scale carps, e.g., taking at least a few hours to achieve a satisfactory solution on a carp instance with thousands of tasks. in this paper, an efficient and scalable approach is propo ...201627514069
carbonic anhydrases in cnidarians: novel perspectives from the octocorallian corallium rubrum.although the ability to elaborate calcium carbonate biominerals was apparently gained independently during animal evolution, members of the alpha carbonic anhydrases (α-cas) family, which catalyze the interconversion of co2 into hco3-, are involved in the biomineralization process across metazoans. in the mediterranean red coral corallium rubrum, inhibition studies suggest an essential role of cas in the synthesis of two biominerals produced in this octocoral, the axial skeleton and the sclerite ...201627513959
benchmark dataset for undirected and mixed capacitated arc routing problems under time restrictions with intermediate facilities.in this article we present benchmark datasets for the mixed capacitated arc routing problem under time restrictions with intermediate facilities (mcarptif). the problem is a generalisation of the capacitated arc routing problem (carp), and closely represents waste collection routing. four different test sets are presented, each consisting of multiple instance files, and which can be used to benchmark different solution approaches for the mcarptif. an in-depth description of the datasets can be f ...201627508252
effects of acute exposure to sublethal waterborne cadmium on energy homeostasis in silver carp (hypophthalmichthys molitrix).effects of acute exposure to sublethal waterborne cadmium (cd) on energy homeostasis in filter-feeding fishes have rarely been studied. the response patterns of energy substances were investigated in juvenile silver carp (hypophthalmichthys molitrix) exposed to sublethal waterborne cd for 96 h. the results showed the 96hlc50 of cd on juvenile silver carp was 1.723 mg/l. sublethal acute exposure of cd significantly affected the energy homeostasis of juvenile silver carp, including increase in pla ...201627488982
nuclear internal transcribed spacer-1 as a sensitive genetic marker for environmental dna studies in common carp cyprinus carpio.the recently developed environmental dna (edna) analysis has been used to estimate the distribution of aquatic vertebrates by using mitochondrial dna (mtdna) as a genetic marker. however, mtdna markers have certain drawbacks such as variable copy number and maternal inheritance. in this study, we investigated the potential of using nuclear dna (ncdna) as a more reliable genetic marker for edna analysis by using common carp (cyprinus carpio). we measured the copy numbers of cytochrome b (cytb) ge ...201727487846
mri-detected osteitis is not associated with the presence or level of acpa alone, but with the combined presence of acpa and rf.in rheumatoid arthritis (ra) bone marrow edema (bme, osteitis) and anti-citrullinated protein antibodies (acpa) are associated with radiographic progression. acpa have been associated with bme, but it is unknown if this association is confined to acpa and bme. we performed cross-sectional analysis of the association of acpa, rheumatoid factor (rf) and anti-carbamylated protein (anti-carp) antibodies with bme and other types of inflammation (synovitis, tenosynovitis) detected by magnetic resonanc ...201627485323
retraction notice to "carrageenan: a natural seaweed polysaccharide and its applications" [carp 105 (2014) 97-112]. 201627474681
effects of carbon dioxide on juveniles of the freshwater mussel (lampsilis siliquoidea [unionidae]).carbon dioxide (co2 ) has shown promise as a tool to control movements of invasive asian carp, but its effects on native freshwater biota have not been well studied. the authors evaluated lethal and sublethal responses of juvenile fatmucket (lampsilis siliquoidea) mussels to co2 at levels (43-269 mg/l, mean concentration) that bracket concentrations effective for deterring carp movement. the 28-d lethal concentration to 50% of the mussels was 87.0 mg/l (95% confidence interval [ci] 78.4-95.9) an ...201727466973
fish gut microbiota analysis differentiates physiology and behavior of invasive asian carp and indigenous american fish. 201627458892
differential enrichment of ttf-i and tip5 in the t-like promoter structures of the rdna contribute to the epigenetic response of cyprinus carpio during environmental adaptation.to ensure homeostasis, ectothermic organisms adapt to environmental variations through molecular mechanisms. we previously reported that during the seasonal acclimatization of the common carp cyprinus carpio, molecular and cellular functions are reprogrammed, resulting in distinctive traits. importantly, the carp undergoes a drastic rearrangement of nucleolar components during adaptation. this ultrastructural feature reflects a fine modulation of rrna gene transcription. specifically, we identif ...201627458840
biomarkers in rheumatoid arthritis, what is new?rheumatoid arthritis (ra) is a chronic inflammatory disease with autoimmune pathogenesis. it affects mainly small joints (of the hands and feet) and has many systemic manifestations. studying biomarkers in rheumatology intensely appeared from the need to understand the mechanisms underlying some rheumatic diseases. discovering new biomarkers with key roles in various stages of evolution, remains a subject of interest for ra. currently, according to the eular 2010 criteria, the rheumatoid factor ...201727453744
carp edema virus in polish aquaculture - evidence of significant sequence divergence and a new lineage in common carp cyprinus carpio (l.).fish samples initially collected by local veterinarians on the common and koi carp farms in poland between 2013 and 2015 as part of a khv surveillance programme, when the water temperature was between 16 and 26 °c, and were also tested for cev by qpcr. a partial 478 nucleotide fragment of the 4a gene was subsequently generated from 17 qpcr-positive common carp cyprinus carpio samples from 36 farm sites tested during the period. sequence alignments and analysis revealed the presence of cev in pol ...201727453481
occurrence, pathology, and ultrastructure of iridovirus and cytoplasmic polyhedrosis viruses in daphnids from the czech republic.iridescent (ivs, family iridoviridae, genus iridovirus) and cytoplasmic polyhedrosis viruses (cpvs; family reoviridae, genus cypovirus) are well known in insects, with thirteen iv species recognized from various orders, and sixteen cpv species known from lepidopterans. in 1975, an iv and cpv were reported in the daphnid, simocehpalus expinosus, in florida, but other reported daphnid virus infections seem to be rare. here we report infected daphnids from woodland and carp ponds in the czech repub ...201627449679
erratum: an ultra-high density linkage map and qtl mapping for sex and growth-related traits of common carp (cyprinus carpio). 201627444814
meta-analysis: diagnostic accuracy of anti-carbamylated protein antibody for rheumatoid arthritis.the anti-carbamylated protein (carp) antibody is a novel biomarker that might help in the diagnosis of rheumatoid arthritis (ra). we aim to assess the diagnostic value of anti-carp antibody for ra.201627437936
theory and application of semiochemicals in nuisance fish control.controlling unwanted, or nuisance, fishes is becoming an increasingly urgent issue with few obvious solutions. because fish rely heavily on semiochemicals, or chemical compounds that convey information between and within species, to mediate aspects of their life histories, these compounds are increasingly being considered as an option to help control wild fish. possible uses of semiochemicals include measuring their presence in water to estimate population size, adding them to traps to count or ...201627417504
dynamic apical surface rings in superficial layer cells of koi cyprinus carpio scale epidermis.this study examined the novel ring-shaped structures found in the apical surface of individual cells of the scale epidermis of koi cyprinus carpio. these apical rings are highly dynamic structures with lifetimes ranging from a few to several minutes. while several ring forms were observed, the predominant ring morphology is circular or oval. two distinct ring forms were identified and designated type i and type ii. type i rings have a well-defined outer border that encircles the surface microrid ...201627412047
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