| assessment of genetic variability and relatedness among atypical aeromonas salmonicida from marine fishes, using aflp-fingerprinting. | atypical strains of aeromonas salmonicida are the causal agent of atypical furunculosis or ulcer disease in various fish species, including spotted wolffish anarhichas minor, which is a promising species in the norwegian fish-farming industry. isolates of atypical a. salmonicida comprise a very heterogenous group showing large variety in biochemical, molecular and virulence characteristics. the genetic variability among atypical isolates from wolffish was characterised using amplified fragment l ... | 2002 | 12180702 |
| survival of an aeromonas hydrophila in an artificial mineral water microcosm. | the survival capacity of an aeromonas hydrophila strain (named sb14) isolated from mineral water was investigated in an artificial mineral water microcosm. the bacterial count of this microorganism was compared with two strains of other species from aquatic environments (pseudomonas fluorescens ssd and pseudomonas putida ssc) and a bacterium indicative of faecal pollution (escherichia coli atcc 25922). among the strains, all added to sterile pyrex glass flasks (1 l) to yield a final bacterial co ... | 2002 | 12188142 |
| [in vitro evaluation of antibacterial substances produced by bacteria isolated from different marine organisms]. | bacteria from several groups of marine organisms were isolated and, using direct antibiograms, identified those that produce antibacterial substances, using a human pathogenic strain of staphylococcus aureus atcc6538 as revealing microorganism. bacteria which produce substances that inhibited s. aureus growth were identified through morphological, physiological and biochemical tests. out of 290 bacteria, 54 (18.6%) inhibited the growth of s. aureus, but only 27 survived for identification. bival ... | 2001 | 12189804 |
| [the expression of aeromonas hydrophila ompts gene and the immunogenicity of recombinant ompts]. | primers were designed based on ompts gene reported recently. with the specific primers, one target fragment about 1024 bp lacking the signal sequence of ompts gene was amplified from a. hydrophila genomic dna via pcr. the ompts gene was hyperexpressed using gene fusion expression vector prset system, and the recombinant omp exhibited a size of 39.9 kd with sds-page and western blot analysis, which showed about 51% of total lysate proteins. antibody to the purified recombinant omp reacted not onl ... | 2002 | 12192861 |
| immunisation of indian major carps against aeromonas hydrophila by intraperitoneal injection. | the indian major carps catla catla, labeo rohita and cirrhinus mrigala were immunised against the potent bacterial fish pathogen, aeromonas hydrophila by the intraperitoneal route, in field conditions. two different polyvalent antigen preparations namely, whole cell and extracellular products (ecp) were used. immunisation schedule consisting of a single booster dose given 28 days after priming resulted in a good agglutinating antibody response in the carps. kinetics of the response were similar ... | 2002 | 12201649 |
| structure of the o-polysaccharide of aeromonas hydrophila o:34; a case of random o-acetylation of 6-deoxy-l-talose. | the o-polysaccharide of aeromonas hydrophila o:34 was obtained by mild-acid degradation of the lipopolysaccharide and studied by chemical methods and nmr spectroscopy before and after o-deacetylation. the polysaccharide was found to contain d-man, d-galnac and 6-deoxy-l-talose (l-6dtal), and the following structure of the tetrasaccharide repeating unit was established [carbohydrate structure see text] where 6dtal(i) is o-acetylated stoichiometrically at position-2 and 6dtal(ii) carries no, one o ... | 2002 | 12204621 |
| biosynthesis and properties of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) polymers. | in support of programs to identify polyhydroxyalkanoates with improved materials properties, we report on our efforts to characterize the mechanical and thermal properties of copolyesters of 3-hydroxybutyrate (3hb) and 3-hydroxyhexanoate (3hhx). the copolyesters, having molar fraction of 3hhx ranging from 2.5 to 35 mol % and average molecular weights ranging from 1.15 x 10(5) to 6.65 x 10(5), were produced by fermentation using aeromonas hydrophila and a recombinant strain of pseudomonas putida ... | 2002 | 12217047 |
| conversion of a transmembrane to a water-soluble protein complex by a single point mutation. | proteins exist in one of two generally incompatible states: either membrane associated or soluble. pore-forming proteins are exceptional because they are synthesized as a water-soluble molecule but end up being located in the membrane -- that is, they are nonconstitutive membrane proteins. here we report the pronounced effect of the single point mutation y221g of the pore-forming toxin aerolysin. this mutation blocks the hemolytic activity of the toxin but does not affect its initial structure, ... | 2002 | 12219082 |
| development of an elisa using a recombinant 41 kda partial protein (p45n') for the detection of riemerella anatipestifer infections in ducks. | riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. an indirect enzyme-linked immunosorbent assay (elisa) has been developed to facilitate early detection of r. anatipestifer infection in ducks. the antigen used was a recombinant 41 kda n-terminal fragment (rp45n') of a newly characterized r. anatipestifer potential surface protein, p45, which was expressed in escherichia coli as an n- ... | 2002 | 12220809 |
| monitoring faecal contamination of the thames estuary using a semiautomated early warning system. | the colifast early warning system, based on measuring beta-galactosidase activity (2 h method), was evaluated for monitoring the level of faecal contamination in the upper tidal thames. two trials were performed, one following heavy rain in november 2000, the next during a dry and sunny period in july 2001. in general the beta-galactosidase activity and the two coliform reference methods (recovery following membrane filtration with membrane lauryl sulphate broth (mlsb) and colilert quantitray) w ... | 2002 | 12227600 |
| pcr detection of potentially pathogenic aeromonads in raw and cold-smoked freshwater fish. | development of a pcr assay for detection of aeromonads carrying the hlya and/or aera genes in fish. | 2002 | 12234351 |
| an aeromonas salmonicida type iv pilin is required for virulence in rainbow trout oncorhynchus mykiss. | aeromonas salmonicida expresses a large number of proven and suspected virulence factors including bacterial surface proteins, extracellular degradative enzymes, and toxins. we report the isolation and characterization of a 4-gene cluster, tapabcd, from virulent a. salmonicida a450 that encodes proteins homologous to components required for type iv pilus biogenesis. one gene, tapa, encodes a protein with high homology to type iv pilus subunit proteins from many gram-negative bacterial pathogens, ... | 2002 | 12240967 |
| inhibition of hemolytic activity of aeromonas salmonicida gcat in rainbow trout red blood cells by a monoclonal antibody. | the present study shows that a monoclonal antibody (mab) directed to aeromonas salmonicida glycerophospholipid:cholesterol acyltransferase (gcat) is capable of protecting rainbow trout red blood cells from the cytotoxic effects of the enzyme in vitro. | 2002 | 12240974 |
| bacterial pathogens isolated from cultured bullfrogs (rana castesbeiana). | a commercial bullfrog (rana castesbeiana) operation in south georgia had multiple epizootics of systemic bacterial infections over a 3-year period, 1998-2000. a number of potential pathogens (aeromonas hydrophila, chryseobacterium (flavobacterium) meningosepticum, chryseobacterium (flavobacterium) indolgenes, edwardsiella tarda, citrobacterfreundii, pseudomonas spp., and (streptococcus iniae) were isolated from various tissues. clinically, frogs demonstrated acute onset of torticolis, stupor, an ... | 2002 | 12296400 |
| simultaneous detection of aeromonas salmonicida, flavobacterium psychrophilum, and yersinia ruckeri, three major fish pathogens, by multiplex pcr. | a multiplex pcr assay based on the 16s rrna genes was developed for the simultaneous detection of three major fish pathogens, aeromonas salmonicida, flavobacterium psychrophilum, and yersinia ruckeri. the assay proved to be specific and as sensitive as each single pcr assay, with detection limits in the range of 6, 0.6, and 27 cfu for a. salmonicida, f. psychrophilum, and y. ruckeri, respectively. the assay was useful for the detection of the bacteria in artificially infected fish as well as in ... | 2002 | 12324372 |
| antibiotic resistance patterns of aeromonas spp. isolated from environmental and clinical sources. | microbiologists from the international centre for diarrhoeal disease research, bangladesh (icddr,b) collected water, sediment, plant, and plankton samples from a lake and a river in dhaka, bangladesh to isolate aeromonas spp. they also took clinical isolates from patients at the dhaka hospital of icddr,b. the microbiologists identified at least 154 isolates of aeromonas and analyzed them for antibiotic susceptibility using the standard agar disc diffusion method. 92.8% of the environmental is ... | 1988 | 12342624 |
| persistent diarrhoea still a serious and difficult problem. | in bangladesh, the likelihood of a diarrhea episode becoming chronic ranges from 7.5-23%. if health providers knew the clinical characteristics of the early phase of diarrhea that becomes persistent diarrhea, they could manage that early diarrheal episode to limit its duration and minimize adverse effects. in bangladesh, aggregative adherent escherichia coli (eaggec) is the most common pathogen in initial stools of children with diarrhea. further, shigella, giardia, aeromonas, and rotavirus a ... | 1992 | 12344717 |
| breast feeding may protect children against campylobacter jejuni associated diarrhoea. | to investigate the bacterial etiology of infant diarrhea in karachi, pakistan, 180 rectal swab samples collected from diarrhea patients under 5 years of age and 30 samples from age- and sex-matched healthy children were analyzed. enteropathogenic organisms were isolated from 70 case samples (38.9%). the two most common etiologic agents were campylobacter jejuni (19.4%) and aeromonas hydrophilia (6.1%). salmonella, shigella, and yersina appear to have a limited incidence in this age group. no ... | 1996 | 12346517 |
| necrotizing fasciitis of the upper extremity resulting from a water moccasin bite. | aeromonas hydrophila infection has been described as the cause of necrotizing fasciitis in patients with suppressed immune systems, burns, or trauma in an aquatic setting. we report a case in which severe necrotizing fasciitis involving hand, arm, chest, and lateral side of trunk, along with toxic shock, developed after the patient was bitten by a venomous snake. mixed aerobic and anaerobic bacteria, including a hydrophila, were isolated from the wound culture. the patient was treated with antiv ... | 2002 | 12356121 |
| requirement of n-glycan on gpi-anchored proteins for efficient binding of aerolysin but not clostridium septicum alpha-toxin. | aerolysin of the gram-negative bacterium aeromonas hydrophila consists of small (sl) and large (ll) lobes. the alpha-toxin of gram-positive clostridium septicum has a single lobe homologous to ll. these toxins bind to glycosylphosphatidylinositol (gpi)-anchored proteins and generate pores in the cell's plasma membrane. we isolated cho cells resistant to aerolysin, with the aim of obtaining gpi biosynthesis mutants. one mutant unexpectedly expressed gpi-anchored proteins, but nevertheless bound a ... | 2002 | 12356721 |
| cloning of the class d beta-lactamase gene from burkholderia pseudomallei and studies on its expression in ceftazidime-susceptible and -resistant strains. | ceftazidime is the antibiotic of choice for the treatment of melioidosis. ceftazidime-resistant burkholderia pseudomallei have been identified and beta-lactamase production implicated in resistance. in this study, 25 strains of b. pseudomallei (15 clinical and 10 environmental strains) were examined for their ability to yield mutants that overexpress beta-lactamase. ceftazidime-resistant mutants were selected readily at high frequency and displayed four- to eight-fold increases in the mics of ce ... | 2002 | 12356787 |
| aeromonas culicicola sp. nov., from the midgut of culex quinquefasciatus. | the taxonomic position was examined of three isolates, mtcc 3249t, sh and slh, from the midgut of female culex quinquefasciatus and aedes aegyptii mosquitoes. numbers of cells of these isolates increased 2000-fold after a blood meal of the mosquitoes. 16s rrna gene sequence analysis of the novel strains showed that they were highly homologous to strains of aeromonas. dna-dna hybridization studies showed that dna of strain mtcc 3249t was 96 and 88% similar to that of strains sh and slh, respectiv ... | 2002 | 12361279 |
| staphylococcal scalded-skin syndrome in an adult due to methicillin-resistant staphylococcus aureus. | we report a case of a 71-year-old man with staphylococcal scalded-skin syndrome (ssss). the patient, with a chronic history of diabetes mellitus, was admitted to our hospital with lumbago, and a diagnosis of renal-cell carcinoma with bone metastasis was made. in hospital he had sudden onset of high fever and erythema, followed by the formation of flaccid bullae and exfoliation, with a positive nikolsky sign. methicillin-resistant staphylococcus aureus (mrsa), producing exfoliative toxin b, was i ... | 2002 | 12373491 |
| evidence for a type iii secretion system in aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. we have screened this bacterium with a broad-host-range probe directed against yscv, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type iii secretion system. this has led to the identification of seven open reading frames that encode homologues to proteins functioning within the type iii secretion systems of yersinia sp ... | 2002 | 12374830 |
| effect of superatmospheric oxygen packaging on sensorial quality, spoilage, and listeria monocytogenes and aeromonas caviae growth in fresh processed mixed salads. | atmospheres with o2 levels higher than 70 kpa have recently been suggested as an innovation to modified atmosphere packaging (map) for fresh processed vegetables to maintain sensory quality and safety. in the present work, mixed vegetable salad collected from a commercial processing plant and stored with the map technique was studied. two gas mixtures were actively generated by using an initial o2 concentration of 95 kpa and combined with two plastic films. the low-barrier film permeability for ... | 2002 | 12380740 |
| microbiological investigations of rainwater and graywater collected for toilet flushing. | seven danish rainwater systems were investigated with respect to the microbial water quality. the general microbiological quality (total numbers of bacteria (aodc)), and heterotrophic plate counts on r2a and plate count agar in the toilets supplied with rainwater were approximately the same as in the reference toilets supplied with drinking water. however, in 12 of the 27 analysed samples one or more pathogens were observed (aeromonas sp., pseudomonas aeruginosa, legionella non-pneumophila, camp ... | 2002 | 12381006 |
| the identification of the transcriptional regulator crp in aeromonas hydrophila jmp636 and its involvement in amylase production and the 'acidic toxicity' effect. | the physiological examination of amylase production by aeromonas hydrophila jmp636 and identification of the mechanism of regulation. | 2002 | 12392524 |
| a rifampicin resistance mutation in the rpob gene confers ppgpp-independent antibiotic production in streptomyces coelicolor a3(2). | in streptomyces coelicolor a3(2), deletion of rela or a specific mutation in rplk ( relc) results in an inability to synthesize ppgpp (guanosine 5'-diphosphate 3'-diphosphate) and impairs production of actinorhodin. we have found that certain rifampicin-resistant ( rif) mutants isolated from either rela or relc strains regain the ability to produce actinorhodin at the same level as the wild-type strain, although their capacity to synthesize ppgpp is unchanged. these rif mutants were found to hav ... | 2002 | 12395192 |
| experimental evidence for major histocompatibility complex-allele-specific resistance to a bacterial infection. | the extreme polymorphism found at some major histocompatibility complex (mhc) loci is believed to be maintained by balancing selection caused by infectious pathogens. experimental support for this is inconclusive. we have studied the interaction between certain mhc alleles and the bacterium aeromonas salmonicida, which causes the severe disease furunculosis, in atlantic salmon (salmo salar l.). we designed full-sibling broods consisting of combinations of homozygote and heterozygote genotypes wi ... | 2002 | 12396502 |
| natural antibodies and their significance in active immunization and protection against a defined pathogen in fish. | natural antibody activity against aeromonas salmonicida extracellular a-layer protein (a-protein) showed large individual variations in a farmed group of 101 goldfish (carassius auratus l.). statistical analyses of these variations led us to divide this group into homogeneous high and low naturally active (hna and lna) subgroups. the hna fish were largely protected against experimental infection with a virulent atypical a. salmonicida strain, while 100% morbidity was recorded in the lna group. i ... | 2002 | 12399194 |
| structurally distinct active sites in the copper(ii)-substituted aminopeptidases from aeromonas proteolytica and escherichia coli. | the aminopeptidase from aeromonas proteolytica (aap) was titrated with copper, which bound sequentially at two distinct sites. both the mono- and disubstituted forms of aap exhibited catalytic hyperactivity relative to the native dizinc enzyme. monosubstituted aap exhibited an axial cu(ii) epr spectrum with slight ph dependence: at ph 6.0 g(parallel) = 2.249, g( perpendicular ) = 2.055, and a(parallel)((63/65)cu) = 1.77 x 10(-)(2) cm(-)(1), whereas at ph 9.65 g(parallel) = 2.245, g( perpendicula ... | 2002 | 12405829 |
| granulocyte responses to experimental injection of live and formalin-killed bacteria in carp (cyprinus carpio). | the objective of the study was to investigate the dynamics of changes in number of granulocytes in bacterial infections of carp (cyprinus carpio). carp were inoculated with non-pathogenic or pathogenic bacteria and changes in type i (neutrophils) and type ii granulocyte (basophils/eosinophils) counts in kidney, circulating blood and peritoneal cavity were assessed. after the injection of non-pathogenic bacteria (escherichia coli), the number of type i and ii cells in blood increased after 6-12h, ... | 2002 | 12406659 |
| crystal structure of the (r)-specific enoyl-coa hydratase from aeromonas caviae involved in polyhydroxyalkanoate biosynthesis. | the (r)-specific enoyl coenzyme a hydratase ((r)-hydratase) from aeromonas caviae catalyzes the addition of a water molecule to trans-2-enoyl coenzyme a (coa), with a chain-length of 4-6 carbons, to produce the corresponding (r)-3-hydroxyacyl-coa. it forms a dimer of identical subunits with a molecular weight of about 14,000 and is involved in polyhydroxyalkanoate (pha) biosynthesis. the crystal structure of the enzyme has been determined at 1.5-a resolution. the structure of the monomer consist ... | 2003 | 12409309 |
| antibacterial activity of chitosan against aeromonas hydrophila. | the effect of chitosan on growth and production of haemolysin by aeromonas hydrophila was investigated as well as the effects of temperature, ph, salts and irradiation on the antibacterial activity of chitosan. it was found that chitosan affected growth and haemolysin production of a. hydrophila in varying degrees compared to the control. growth and haemolysin production were clearly suppressed at 0.04% of chitosan. suppression was more effective at ph 6.0 than at ph 7.0. the bactericidal effect ... | 2002 | 12428449 |
| diarrhoeagenic bacterial pathogens in hiv-positive patients with diarrhoea in rural communities of limpopo province, south africa. | potential enteric bacterial pathogens in 60 hiv-positive patients with chronic diarrhoea in rural communities of the limpopo province, south africa, were identified using standard microbiological methods. the kirby-bauer disk-diffusion method was employed to determine antibiograms of isolated bacteria. results revealed that diarrhoeagenic bacterial agents were isolated from 48 (80%) of the 60 hiv-positive patients with diarrhoea. forty-four (73.3%) and 16 (26.7%) of the 60 patients were female a ... | 2002 | 12430759 |
| chain stiffness of heteropolysaccharide from aeromonas gum in dilute solution by dynamic light scattering. | dynamic light scattering measurements have been made on 15 fractions of aeromonas (a) gum, an extracellular heteropolysaccharide produced by the strain aeromonas nichidenii, with dimethylsulfoxide containing 0.2m lithium chloride as the solvent at 25 degrees c. data for the translational diffusion coefficient d covering a molecular weight range from 4.5 x 10(5) to 2.1 x 10(6) and ratios of the z-average radius of gyration <s(2)>(z) (1/2) to the hydrodynamic radius r(h) (calculated with previous ... | 2002 | 12434427 |
| a chemical switch regulates fibrate specificity for peroxisome proliferator-activated receptor alpha (pparalpha ) versus liver x receptor. | fenofibrate is clinically successful in treating hypertriglyceridemia and mixed hyperlipidemia presumably through peroxisome proliferator-activated receptor alpha (pparalpha)-dependent induction of genes that control fatty acid beta-oxidation. lipid homeostasis and cholesterol metabolism also are regulated by the nuclear oxysterol receptors, liver x receptors alpha and beta (lxralpha and lxrbeta). here we show that fenofibrate ester, but not fenofibric acid, functions as an lxr antagonist by dir ... | 2003 | 12441342 |
| the protein encoded at the 3' end of the serine protease gene of aeromonas sobria functions as a chaperone in the production of the protease. | for the successful production of aeromonas sobria serine protease (asp), open reading frame 2 (orf2) protein, encoded at the 3' end of the protease operon, is required. in this study, we examined the action of orf2 protein. the results showed that the protein associated with asp in the periplasm and helped asp to form an active structure. | 2002 | 12446656 |
| [persistent properties of protozoa-associated bacteria]. | the species structure and persistent properties (antilysozyme and antihistone activity) of bacteria forming associations with protozoa is revealed. among them, 68.9% of the isolates were enterobacteria, the remaining organisms belonged to the families aeromonas, alcaligenes, pseudomonas, vibrio, etc. within the family enterobacteriaceae bacteria of the escherichia group prevailed. 50.4% of the isolates were found to have antilysozyme activity and 97%--antihistone activity. the level of persisten ... | 2002 | 12449703 |
| distribution of aeromonas spp. as identified by 16s rdna restriction fragment length polymorphism analysis in a trout farm. | this study used restriction fragment length polymorphism (rflp) with aeromonas-specific primers to identify species of aeromonas and to investigate their distribution in a trout farm and stream. | 2002 | 12452953 |
| phenotypic characteristics and pathogenicity of aeromonas genomospecies isolated from common carp (cyprinus carpio l.). | to evaluate the relationship between the genomospecies, phenotypic profile and pathogenicity for carp of 37 motile aeromonas strains. | 2002 | 12452960 |
| the catalytic domains of thiamine triphosphatase and cyab-like adenylyl cyclase define a novel superfamily of domains that bind organic phosphates. | the cyab protein from aeromonas hydrophila has been shown to possess adenylyl cyclase activity. while orthologs of this enzyme have been found in some bacteria and archaea, it shows no detectable relationship to the classical nucleotide cyclases. furthermore, the actual biological functions of these proteins are not clearly understood because they are also present in organisms in which there is no evidence for cyclic nucleotide signaling. | 2002 | 12456267 |
| rapid detection of columnaris disease in channel catfish (ictalurus punctatus) with a new species-specific 16-s rrna gene-based pcr primer for flavobacterium columnare. | a 16-s rrna gene from the chromosomal dna of the fish-pathogenic bacterium flavobacterium columnare (formerly flexibacter columnaris), strain ars-i, was cloned, sequenced and used to design a polymerase chain reaction (pcr) primer set. the primer set amplified a specific 1193-bp dna fragment from f. columnare strains but not from related bacteria, f. psychrophilum, f. aquatile, f. branchiophilum, or other bacterial pathogens of fish, flexibacter maritimus, cytophaga johnsonae, edwardsiella ictal ... | 2003 | 12459241 |
| an unwanted import to the uk: a carbapenem-resistant clinical isolate of acinetobacter baumannii producing metallo-beta-lactamase. | | 2002 | 12461042 |
| isolation of hexavalent chromium-reducing cr-tolerant facultative anaerobes from tannery effluent. | several facultative anaerobes tolerant to high levels of chromate (>400 mg/ml) were isolated from tannery effluents. these isolates displayed varying degrees of cr(vi) reduction under aerobic and anaerobic conditions at room temperature (24+/-2 degrees c). interestingly, eight isolates were efficient in reducing 70% cr(vi) anaerobically. this includes 5 isolates of genus aerococcus, two isolates of micrococcus and single isolate of genus aeromonas. these isolates were subjected to further charac ... | 2001 | 12483605 |
| induced colistin resistance as an identifying marker for aeromonas phenospecies groups. | to investigate the taxonomic interest of colistin resistance as an identifying marker for aeromonas phenospecies groups. | 2003 | 12485337 |
| prevalence and resistance to antibiotics for aeromonas species from retail fish in malaysia. | a total of 87 market fish samples representing five types of fish were evaluated for the presence of aeromonas spp. of the samples examined, 69%, 55%, 11.5% and 2.3% harbored aeromonas spp., a. veronii biovar sobria, a. hydrophila and a. caviae, respectively. the 60 isolated aeromonas spp. strains were further examined for hemolytic activity, resistance to antimicrobial agents and presence of plasmids. hemolytic activity varied widely among the isolated strains. though all the isolates demonstra ... | 2003 | 12485753 |
| phylogenetic study and identification of human pathogenic vibrio species based on partial hsp60 gene sequences. | the use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. a 600-bp partial hsp60 gene was amplified by pcr and sequenced from 29 strains representing 15 vibrio species within the family vibrionaceae. sequence comparison of the amplified partial hsp60 gene revealed 71-82% sequence identity among different vibrio species and 96-100% sequence identity among epidemiologically distinct strains with the same species designation. this degre ... | 2002 | 12489780 |
| antibacterial activity of mappia foetida leaves and stem. | successive petroleum ether, chloroform and methanol extracts of mappia foetida leaves and stem were tested for their antibacterial activity. the methanol fractions were found to be most effective against all the tested organisms. | 2002 | 12490245 |
| a dna probe specific for aeromonas colonies. | members of the genus aeromonas are important enteropathogens. commercial identification systems are often unable to correctly identify aeromonas strains and misidentification as vibrio spp. is common. a digoxigenin-dna probe based on a 237 bp of the glycerophospholipid-cholesterol acyltransferase gene has been tested in a colony hybridization assay. the probe hybridized with all aeromonas species tested (n = 16) but not with strains of other enteropathogenic bacteria (n = 20). the probe allowed ... | 2002 | 12493167 |
| [clinical and microbiological characteristics of spontaneous bacterial peritonitis (sbp) in a recent five year period]. | recently, treatment failure with the third generation of cephalosporin was increasingly noted in patients with spontaneous bacterial peritonitis (sbp). we therefore were to evaluate the pattern of antibiotic resistance and its clinical significance. | 2002 | 12499818 |
| lipase activity and gene cloning of acinetobacter calcoaceticus lp009. | the production of lipase from acinetobacter calcoaceticus lp009, a bacterium isolated from raw milk, was found to be best induced by tween-80 at 1.0% concentration. it was efficiently secreted, and only a minute amount of activity was detected at the cell surface and intracellularly. a. calcoaceticus lp009 lipase exhibited maximum activity at ph 7.0 and 50 degrees c, and was relatively stable upon storage at ph 5.0 to 7.0 and at 4, 30, or 37 degrees c. the enzyme was found to be inactivated by e ... | 1998 | 12501281 |
| polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria. | polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ... | 1997 | 12501353 |
| specific probiotic characterization of weissella hellenica ds-12 isolated from flounder intestine. | a total of 199 microorganisms were isolated from the intestinal contents of flounder (paralichthys olivaceus) in a fish farm in seoul, korea. among these strains, ds-12 was selected as a candidate for flounder probiotics because of its excellent exhibition of antimicrobial activity against fish pathogens such as edwardsiella, pasteurella, aeromonas, and vibrio, and initiate growth in 10% nacl, 10% bile, and in broth at ph 3 for 90 min. this strain was gram-positive, and catalase-negative coccoid ... | 1998 | 12501410 |
| dna-dna reassociation and phenotypic data indicate synonymy between aeromonas enteropelogenes schubert et al. 1990 and aeromonas trota carnahan et al. 1991. | mainly on the basis of phylogenetic and genotypic evidence, it has been suggested previously that the species aeromonas enteropelogenes schubert et al. 1990 is identical to the species aeromonas trota carnahan et al. 1991. probably because the description of a. enteropelogenes preceded the proposal of a. trota by only a few months, dna-dna hybridizations were never performed between representative strains of these two taxa. in the present study, new dna-dna hybridizations between the type strain ... | 2002 | 12508855 |
| [a colorimetric method of hec toxin hemolysis test for diagnosis of paroxysmal nocturnal hemoglobinuria]. | the purpose of the study is to establish a colorimetric method of hec toxin hemolysis test for diagnosis of paroxysmal nocturnal hemoglobinuria (pnh). rbcs from normal persons and patients with pnh and non-pnh anemia were treated with hec toxin secreted by aeromonas hydrophila j-1 strain and the absorbance at 630 nm was measured to quantitate the extent of hemolysis. the results demonstrated that the rbcs from pnh patients showed resistance to the toxin hemolysis, which was in accord with the pe ... | 2002 | 12513727 |
| differential growth response of colony-forming alpha- and gamma-proteobacteria in dilution culture and nutrient addition experiments from lake kinneret (israel), the eastern mediterranean sea, and the gulf of eilat. | even though it is widely accepted that bacterioplankton growth in lakes and marine ecosystems is determined by the trophic status of the systems, knowledge of the relationship between nutrient concentrations and growth of particular bacterial species is almost nonexistent. to address this question, we performed a series of culture experiments with water from lake kinneret (israel), the eastern mediterranean sea, and the gulf of eilat (northern red sea). in the initial water samples, the proporti ... | 2003 | 12513996 |
| a colonization factor (production of lateral flagella) of mesophilic aeromonas spp. is inactive in aeromonas salmonicida strains. | the nine laf (lateral flagellum) genes of mesophilic aeromonads are in the aeromonas salmonicida genome. the laf genes are functional, except for lafa (flagellin gene), which was inactivated by transposase 8 (is3 family). a pathogenic characteristic of mesophilic aeromonads (lateral flagella) is abolished in this specialized pathogen with a narrow host range. | 2003 | 12514057 |
| molecular typing of aeromonas isolates in natural mineral waters. | a total of 103 isolates of aeromonas spp. were obtained over a 3-year period from a natural mineral water and from surface streams located within the boundaries of the watershed of the natural mineral water wells and were typed by macrorestriction analysis of genomic dna with xbai and by pulsed-field gel electrophoresis. all aeromonas caviae isolates from the natural mineral water belonged to the same clone, and an analogous clonal identity was found among aeromonas hydrophila isolates. these tw ... | 2003 | 12514065 |
| temperature effect on the immune defense functions of arctic charr salvelinus alpinus. | the arctic charr salvelinus alpinus is an endangered fish species in finland, and thus farming is carried out mainly for stocking purposes. farmed charr are susceptible to infection with atypical aeromonas salmonicida (aas). losses of valuable brood stock will severely reduce the genetic diversity of stocked charr. no commercial vaccines are available to prevent aas infection, and vaccines against furunculosis (caused by typical a. salmonicida, tas) do not protect the charr against aas infection ... | 2002 | 12517005 |
| isolation and characterization of aeromonas species from an eutrophic tropical estuary. | isolation and characterization of aeromonas species were undertaken in the ebrié lagoon (abidjan, côte d'ivoire) over a one year period. overall 63% of 501 water samples were identified as positive for aeromonas spp. a. sobria, a. hydrophila and a. caviae represent respectively 49.20%, 20.63% and 30.15% of the positive samples. thirty nine of the positive samples were recovered from surface water samples; while 24 bottom water samples were positive for aeromonas. aeromonas species are most frequ ... | 2002 | 12523537 |
| resistance to quaternary ammonium compounds in food-related bacteria. | microbial resistance to antimicrobial agents continues to be a major problem. the frequent use and misuse of disinfectants based on quaternary ammonium compounds (qacs) in food-processing industries have imposed a selective pressure and may contribute to the emergence of disinfectant-resistant microorganisms. a total number of 1,325 gram-negative isolates (escherichia coli, other coliforms vibrio spp., and aeromonas spp.) and 500 enterococcus spp. from food and food-processing industries and fis ... | 2002 | 12523638 |
| decolorization of the textile dyes by newly isolated bacterial strains. | six bacterial strains with the capability of degrading textile dyes were isolated from sludge samples and mud lakes. aeromonas hydrophila was selected and identified because it exhibited the greatest color removal from various dyes. although a. hydrophila displayed good growth in aerobic or agitation culture (agi culture), color removal was the best in anoxic or anaerobic culture (ana culture). for color removal, the most suitable ph and temperature were ph 5.5-10.0 and 20-35 degrees c under ano ... | 2003 | 12523970 |
| a comparative study of carboxyfluorescein diacetate and carboxyfluorescein diacetate succinimidyl ester as indicators of bacterial activity. | staining bacteria with esterified fluorogenic substrates followed by flow cytometric analysis offers a means for rapid detection of metabolically active bacteria. flow cytometry (fcm) was used to assess carboxyfluorescein diacetate (cfda) and carboxyfluorescein diacetate succinimidyl ester (cfda/se) as indicators of bacterial activity for cultured bacteria, including aeromonas hydrophila, bacillus subtilis, escherichia coli, pseudomonas aeruginosa, staphylococcus epidermidis and bacteria from en ... | 2003 | 12531507 |
| degradation of benzyldimethylalkylammonium chloride by aeromonas hydrophila sp. k. | the aim of this work was to study the biodegradation of benzyldimethylalkylammonium chloride (bac) by aeromonas hydrophila sp. k, an organism isolated from polluted soil and capable of utilizing bac as sole source of carbon and energy. | 2003 | 12534818 |
| sensitivity of polarized epithelial cells to the pore-forming toxin aerolysin. | aerolysin is one of the major virulence factors produced by aeromonas hydrophila, a human pathogen that produces deep wound infection and gastroenteritis. the toxin interacts with target mammalian cells by binding to the glycan core of glycosylphosphatidyl inositol (gpi)-anchored proteins and subsequently forms a pore in the plasma membrane. since epithelial cells of the intestine are the primary targets of aerolysin, we investigated its effect on three types of polarized epithelial cells: caco- ... | 2003 | 12540553 |
| microbial agents associated with waterborne diseases. | many classes of pathogens excreted in feces are able to initiate waterborne infections. there are bacterial pathogens, including enteric and aquatic bacteria, enteric viruses, and enteric protozoa, which are strongly resistant in the water environment and to most disinfectants. the infection dose of viral and protozoan agents is lower than bacteria, in the range of one to ten infectious units or oocysts. waterborne outbreaks of bacterial origin (particularly typhoid fever) in the developing coun ... | 2002 | 12546197 |
| [production, partial purification and characterization of methamidophos-degrading enzyme from methylotroph wb-1]. | after cultivation for 20 h in the inorganic salt medium with methamidophos as sole carbon and nitrogen source, methylotroph wb-1 could produce methamidophos-degrading enzyme in larger amounts. the enzyme was partially purified by sonication disruption, tween-20 extraction, heat treatment(9 min at 60 degrees c), deae-cellulose 32 and cm-cellulose32 chromatography with 22.8 times purification and 78.8% recovery. activity staining showed single violate band corresponding to that of protein staining ... | 2000 | 12548765 |
| [purification and identification of siderophore from aeromonas hydrophila]. | siderophore of aeromonas hydrophila(ah) j-1 was purified from culture supernatant by polyamide column chromatography, eluted by double-distilled water, ethyl acetate precipitated, lyophilized, then the white powder was collected, and demonstrated by cas and arnow assay. the siderophore was phenolate, composed of 2,3-dihydroxybenzoic acid (2,3-dhb), lysine, glycine and tryptophan by hplc. being cultured iniron-deficient medium, the purified siderophore could stimulated the growth of ah n9a which ... | 2000 | 12548885 |
| [gene cloning and expression of prolyl endopeptidase from aeromonas punctata]. | prolyl endopeptidase gene was cloned from aeromonas punctata subsp. punctata(st-78-3-3) using activity screening method and the 3.3 kb dna fragment containing pep gene was sequenced. dna sequence from 20-2092 bp was orf region coding 690 amino acids of prolyl endopeptidase. it was a new pep gene through gene search. the genetic engineered e. coli bl21/pgem-pep overexpressing recombinant aeromonas punctata prolyl endopeptidase(appep) was constructed. cultured in yh medium, the expressed appep was ... | 2000 | 12548992 |
| inhibitory activity of spices and essential oils on psychrotrophic bacteria. | this study was designed to evaluate "in vitro" the inhibitory effects of spices and essential oils on the growth of psycrotrophic food-borne bacteria: aeromonas hydrophila, listeria monocytogenes and yersinia enterocolitica. the sensitivity to nine spices and their oils (chilli, cinnamon, cloves, ginger, nutmeg, oregano, rosemary, sage, thyme) was studied. antibacterial activity was evaluated on liquid and solid medium. spices: 1% concentration of each spice was added separately to triptic soy b ... | 2003 | 12578319 |
| aeromonas sobria corneal ulcer. | | 2003 | 12579184 |
| high antigenic cross-reaction among the bacterial species responsible for diseases of cultured freshwater fishes and strategies to overcome it for specific serodiagnosis. | antigenic sharing among the most commonly bacterial pathogens such as aeromonas hydrophila, edwardsiella tarda and pseudomonas fluorescens of indian major carps has been studied using immunological reactions such as cross-agglutination, disc diffusion and indirect enzyme linked immunosorbent assay (elisa). the data were analysed using statistical analysis (sas), version 6.12. the results showed high antigenic similarities among the bacterial whole cells, whole cell lysates, somatic 'o' antigens, ... | 2003 | 12581749 |
| the genetics of glycosylation in gram-negative bacteria. | in recent years there has been a dramatic increase in reports of glycosylation of proteins in various gram-negative systems including neisseria meningitidis, neisseria gonorrhoeae, campylobacter jejuni, pseudomonas aeruginosa, escherichia coli, caulobacter crescentus, aeromonas caviae and helicobacter pylori. although this growing list contains many important pathogens (reviewed by benz and schmidt [mol. microbiol. 45 (2002) 267-276]) and the glycosylations are found on proteins important in pat ... | 2003 | 12586395 |
| solar inactivation of mesophilic aeromonas by exogenous photooxidation in high-rate algal pond treating waste water. | investigations were carried out to observe the effect of sunlight on the survival of mesophilic aeromonas (a. caviae, a. hydrophila and a. sobria) in high-rate algal pond. | 2003 | 12588563 |
| general function of n-terminal propeptide on assisting protein folding and inhibiting catalytic activity based on observations with a chimeric thermolysin-like protease. | pro-aminopeptidase processing protease (pa protease) is a thermolysin-like metalloprotease produced by aeromonas caviae t-64. the n-terminal propeptide acts as an intramolecular chaperone to assist the folding of pa protease and shows inhibitory activity toward its cognate mature enzyme. moreover, the n-terminal propeptide strongly inhibits the autoprocessing of the c-terminal propeptide by forming a complex with the folded intermediate pro-pa protease containing the c-terminal propeptide (mc). ... | 2003 | 12589825 |
| studies of the mechanism of action of the aerolysin-like hemolysin of aeromonas sobria in stimulating t84 cells to produce cyclic amp. | we previously reported that the aerolysin-like hemolysin of aeromonas sobria stimulates t84 cells to produce cyclic amp, which then emerges in the culture medium. in order to clarify the mechanism of action of the hemolysin, we examined the involvement of adenosine nucleotide. the results show that the hemolysin stimulates t84 cells to release atp, which is then converted to adenosine by ectonucleotidase. the adenosine generated might stimulate the p1 adenosine receptors of t84 cells to produce ... | 2003 | 12595476 |
| distribution of aeromonas hydrophila serogroups in different clinical samples and the development of polyclonal antibodies for rapid identification of the genus aeromonas by direct agglutination. | we characterized a collection of 256 aeromonas hydrophila strains isolated from blood, discharge and stool for their serogroup designation. of these, 2.3% were untypable and 15.2% were rough strains. among the typable strains, about 50% comprised serogroups o:11, o:16, o:18, o:34 and o:83. to develop rapid differentiation of aeromonas from other oxidase-positive bacteria, antisera against aeromonas were produced to establish a direct, genus-specific, agglutination test. it was found that among 1 ... | 2002 | 12597362 |
| immunomodulation by dietary vitamin c in healthy and aflatoxin b1-induced immunocompromised rohu (labeo rohita). | the aim of this study was to examine the immunomodulatory effect of high levels of dietary vitamin c in healthy and immunocompromised rohu (labeo rohita) treated with aflatoxin b1 (afb1). four groups of rohu were fed experimental diets containing either no vitamin c or supplemented with vitamin c at 500 ppm for 60 days. on the first day of feeding, one group fed the high vitamin c diet and one fed the vitamin c deficient diet, were injected intraperitoneally with a single doses of afb1 at 1.25 m ... | 2003 | 12602688 |
| aeromonas isolates from human diarrheic stool and groundwater compared by pulsed-field gel electrophoresis. | gastrointestinal infections of aeromonas species are generally considered waterborne; for this reason, aeromonas hydrophila has been placed on the united states environmental protection agency contaminant candidate list of emerging pathogens in drinking water. in this study, we compared pulsed-field gel electrophoresis patterns of aeromonas isolates from stool specimens of patients with diarrhea with aeromonas isolates from patients' drinking water. among 2,565 diarrheic stool specimens submitte ... | 2003 | 12603994 |
| cloning, dna sequence, and expression of aeromonas caviae ws7b chitinase gene. | a chitinase-producing bacterium, designated ws7b, was isolated from a soil sample obtained from a black-pepper plantation on bangka island, indonesia. fatty-acid methyl-ester analysis indicated that the isolate was aeromonas caviae. a chitinase gene from ws7b was cloned in a puc19-based plasmid vector, but without its natural promoter. the complete nucleotide sequence of the gene was determined, and the structural gene consisted of a 2748-bp region encoding 864 amino acids. dna sequence analysis ... | 2003 | 12611264 |
| [presence and meaning of aeromonas in drinkable water] | many species of aeromonas are thought to be the cause of diarrhoeal syndromes, bactaeremia and eye infections. recent studies have shown that the presence of aeromonas in drinkable water is not associated with that of feacal indicators. the authors have tested 62 samples of drinkable water from various wells, as well as 14 samples of two mineral waters collected in eastern sicily from 1996 to 1999. aeromonas were found in 14 (18%) out of 76 samples. also, biochemical detection of the strains sho ... | 2002 | 12616271 |
| characterization of microbial communities from coastal waters using microarrays. | molecular methods, including dna probes, were used to identify and enumerate pathogenic vibrio species in the chesapeake bay; our data indicated that vibrio vulnificus exhibits seasonal fluctuations in number. our work included a characterization of total microbial communities from the bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. to identify members of the microbial community, we amplified ... | 2003 | 12620025 |
| detection of erythrocytes deficient in glycosylphosphatidyl-inositol anchored membrane proteins in patients with paroxysmal nocturnal hemoglobinuria by the toxin hec secreted by aeromonas hydrophila j-1. | to study the feasibility of diagnosing paroxysmal nocturnal hemoglobinuria (pnh) with toxin hec, the abbreviation of hemolytic, entreotoxigenicity and cytotoxity secreted by aeromonas hydrophila j-1. | 2003 | 12623426 |
| detection and characterization of the hemolysin genes in aeromonas hydrophila and aeromonas sobria by multiplex pcr. | a multiplex pcr assay was designed to amplify the aeromonas hydrophila and a. veronii bv. sobria hemolysin and aerolysin genes. the assay was evaluated by using 121 clinical isolates and 7 reference strains of aeromonas spp., and these were divided into five genotypes on the basis of the results of the multiplex pcr. the five genotypes were characterized as type 1 for those carrying the ahh1 gene only (36% of isolates), type 2 for those carrying the asa1 gene only (8.5% of isolates), type 3 for ... | 2003 | 12624028 |
| niche-specific association of aeromonas ribotypes from human and environmental origin. | a total of 88 aeromonas isolates from distinct locations and sources (39 from extraintestinal infections, 31 from diarrhoeic, ten from non-diarrhoeic faeces, all human, and eight from fresh water) were subjected to phenospecies identification, serotyping, ribotyping and detection of some virulence markers. the strains belonged to four different phenospecies marked by 19 o serogroups and 38 ribotypes. no strong correlation between these parameters was found, and no group, as defined by the typing ... | 2003 | 12636248 |
| scope of potential bacterial agents of diarrhoea and microbial assessment of quality of river water sources in rural venda communities in south africa. | the microbial quality of several, usually untreated, surface domestic water sources, used by rural communities in the venda region of south africa, was assessed to gauge their fitness for human consumption and to highlight the possible impact of waterborne diseases. the water sources studied were six points on the levubu river and the rivers mutale, ngwedi, tshinane, makonde, mutshindudi and mudaswali. total and faecal coliform, heterotrophic bacteria, enterococci and coliphage counts were used ... | 2003 | 12639006 |
| bacteriocin-like substance (bls) production in aeromonas hydrophila water isolates. | 30 aeromonas hydrophila water isolates were tested for bacteriocin-like substance (bls) production using a target panel of closely related microorganisms and other gram-positive and gram-negative bacteria, including food-borne pathogens. a. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. in particular all a. hydrophila showed antibacterial activity against one or more of the ... | 2003 | 12644237 |
| bacteraemia in high-risk patients. | in this study, we noticed a high incidence of bacteraemia in high-risk patients especially due to nonfermenter gram negative bacilli (nfgnb) and coagulase negative staphylococci (cons). bacteraemia caused by some rare bacteria such as moraxella spp., aeromonas spp., flavobacterium meningosepticum was also noted during the study. antibiotic resistance pattern showed that many isolates were multi drug resistant (mdr). this can be attributed to nosocomial-infection, which may occur due to more and ... | 2002 | 12645165 |
| integron-containing incu r plasmids pras1 and par-32 from the fish pathogen aeromonas salmonicida. | a 45-kb r plasmid, pras1, that confers resistance to tetracyclines, trimethoprim, and sulfonamides was isolated in 1989 from an atypical strain of the fish pathogen aeromonas salmonicida. this plasmid could be transferred by conjugation to escherichia coli with a high degree of efficiency (frequency, 0.48). the following year pras1 was isolated from a. salmonicida subsp. salmonicida in the same area. incompatibility group u plasmid pras1 contained a drug resistance-determining region of 12 kb co ... | 2003 | 12654659 |
| effect of diuron on aquatic bacteria in laboratory-scale wastewater treatment ponds with special reference to aeromonas species studied by colony hybridization. | six laboratory-scale wastewater treatment ponds were filled with sediment and water obtained from a reference pond (a wastewater treatment plant located in a rural environment at montel-de-gelat, puy-de-dôme, france). they were kept at 20 degrees c, with alternative light and dark periods (12 h-12 h), and fed with raw effluent supplied weekly. three of them were treated with diuron (dissolved in dmso) at a final concentration 10 mg/l, while the other three received only dmso. physico-chemical pa ... | 2003 | 12656266 |
| differential expression of the enolase gene under in vivo versus in vitro growth conditions of aeromonas hydrophila. | aeromonas hydrophila is an emerging human pathogen that leads to gastroenteritis and other invasive diseases. by using a murine peritoneal culture (mpc) model, we identified via restriction fragment differential display pcr (rfddpcr) five genes of a. hydrophila that were differentially expressed under in vivo versus in vitro growth conditions. the gene encoding enolase was among those five genes that were differentially up regulated. enolase is a glycolytic enzyme and its surface expression was ... | 2003 | 12668143 |
| coordination geometries of metal ions in d- or l-captopril-inhibited metallo-beta-lactamases. | d- and l-captopril are competitive inhibitors of metallo-beta-lactamases. for the enzymes from bacillus cereus (bcii) and aeromonas hydrophila (cpha), we found that the mononuclear enzymes are the favored targets for inhibition. by combining results from extended x-ray absorption fine structure, perturbed angular correlation of gamma-rays spectroscopy, and a study of metal ion binding, we derived that for cd(ii)1-bcii, the thiolate sulfur of d-captopril binds to the metal ion located at the site ... | 2003 | 12668674 |
| influence of polarisation and differentiation on interaction of 43-kda outer-membrane protein of aeromonas caviae with human enterocyte-like caco-2 cell line. | it has been recognised that adherence and invasion to host cells are important steps in the pathogenesis of entero-pathogenic bacteria, including aeromonas caviae. however, the virulence factors of a. caviae remain, for the most part, poorly known. this study examined the interaction of a. caviae isolates to caco-2 cells in different polarisation and differentiation conditions. the adherence of a. caviae may be related to accessibility of host cell basolateral receptors. aggregative a. caviae is ... | 2003 | 12684708 |
| detection and genetic analysis of group ii capsules in aeromonas hydrophila. | the genetic organization and sequences of the group ii capsule gene cluster of aeromonas hydrophila ppd134/91 have been determined previously. the purified capsular polysaccharides can increase the ability of avirulent strain ppd35/85 to survive in naive tilapia serum but have no inhibitory effect on the adhesion of ppd134/91 to carp epithelial cells. in this study, the presence of group ii capsules among 33 randomly chosen a. hydrophila strains was examined by electron microscopy and genetic an ... | 2003 | 12686647 |
| rotavirus type a and other enteric pathogens in stool samples from children with acute diarrhea on the colombian northern coast. | the present study, conducted from march 1998 to july 2000, determined the etiology of acute diarrhea in 253 young children and infants from cartagena and sincelejo, colombia. in 253 stool samples, the following enteric pathogens were recovered: rotavirus type a (36.6%) as the major agent, salmonella spp (9.0%), shigella spp (8.0%), enteric pathogenic escherichia coli (6.0%), enteric hemorragic esc. coli (2.8%), providencia alcalifaciens (2.8%), aeromonas hydrophila (2.0%), yersinia enterocolitic ... | 2003 | 12687410 |
| study of the effect of the midgut bacterial flora of culex quinquefasciatus on the susceptibility of mosquitoes to japanese encephalitis virus. | culex quinquefasciatus mosquitoes were collected from the field and microbial flora was isolated from their midgut. isolates belonging to 13 different genera were obtained. four most abundant isolates viz. pseudomonas sp., acinetobacterjunii, staphylococcus epidermis and aeromonas culicicola were used to study their effect on the susceptibility of mosquitoes to japanese encephalitis virus (jev). incorporation of individual isolates in the mosquito bloodmeal resulted in an increased susceptibilit ... | 2002 | 12693864 |
| molecular cloning, sequencing and characterization of omp48, the gene encoding for an antigenic outer membrane protein from aeromonas veronii. | to clone, sequence and characterize the gene encoding the omp48, a major outer membrane protein from aeromonas veronii. | 2003 | 12694457 |
| enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) via manipulating the fatty acid beta-oxidation pathway in e. coli. | acyl-coa dehydrogenase gene (yafh) of escherichia coli was expressed together with polyhydroxyalkanoate synthase gene (phac(ac)) and (r)-enoyl-coa hydratase gene (phaj(ac)) from aeromonas caviae. the expression plasmids were introduced into e. coli jm109, dh5 alpha and xl1-blue, respectively. compared with the strains harboring only phac(ac) and phaj(ac), all recombinant e. coli strains harboring yafh, phac(ac) and phaj(ac) accumulated at least four times more poly(3-hydroxybutyrate-co-3-hydroxy ... | 2003 | 12694916 |
| transferrin and the innate immune response of fish: identification of a novel mechanism of macrophage activation. | we have previously demonstrated that a non-cytokine serum protein called transferrin was a primary activating molecule of the goldfish (carassius auratus) macrophage antimicrobial response. the ability of the enzymatically cleaved forms of this protein to modulate fish macrophage function is novel and may represent a primitive and evolutionary conserved mechanism for the induction of no response of macrophages. in the present study we confirm our earlier findings using immunoaffinity purified go ... | 2003 | 12697311 |