| acute cholangitis. | cholangitis is an infection of the biliary ductal system that results from the combination of bactibilia and biliary obstruction. choledocholithiasis has been the leading cause of cholangitis. however, in recent years, especially at tertiary referral centers, nonoperative biliary manipulations, often in patients with unresectable malignancies, have become the most common cause of cholangitis. as a result, the complete triad of fever and chills, jaundice, and abdominal pain, as originally describ ... | 1990 | 2247816 |
| cefpodoxime: comparable evaluation with other orally available cephalosporins. with a note on the role of beta-lactamases. | the antibacterial activity of cefpodoxime, a new orally active methoxy-imino cephalosporin was evaluated in 470 recent isolates of gram-positive cocci and gram-negative rods from clinical specimens and compared to that of other orally active beta-lactam compounds. cefpodoxime was highly active against ampicillin-resistant enterobacteria producing the plasmid-mediated tem-1, tem-2 or oxa-1 enzymes, as was the case for the other newer compounds. however, it was poorly active against cefuroxime-res ... | 1990 | 2248685 |
| [the immunobiological properties of the antigenic preparations obtained from a vaccinal strain of klebsiella pneumoniae 204 grown in media of differing compositions]. | in the technology of the cultivation of k. pneumoniae vaccine strain 204 with a view to obtaining biomass for the production of antigenic preparations the traditionally used culture medium with full nutritional value has been replaced by the alternative variant of synthetic medium. the specific physiological and morphological features of this strain grown in synthetic culture medium have been studied and described. irrespective of the composition of the culture media used for cultivation, the an ... | 1990 | 2251890 |
| [a clinical trial of the preparation palin in women with refractory colpitis]. | the authors carried out clinical trial of the preparation palin (pipemidinic acid) on 43 women with persistent colpitis after preliminary testing. there was negative microbiological finding in 67% of women. the results of the authors differed from those of other authors, since palin was used mainly in women with chronic colpitis resistant to routine treatment. minimal side effects were found. usage of palin is recommended in women with colpitis, caused by: e. coli, enterococcus, klebsiella and m ... | 1990 | 2252156 |
| homology among bacterial catalase genes. | catalase activities in crude extracts of exponential and stationary phase cultures of various bacteria were visualized following gel electrophoresis for comparison with the enzymes from escherichia coli. citrobacter freundii, edwardsiella tarda, enterobacter aerogenes, klebsiella pneumoniae, and salmonella typhimurium exhibited patterns of catalase activity similar to e. coli, including bifunctional hpi-like bands and a monofunctional hpii-like band. proteus mirabilis, erwinia carotovora, and se ... | 1990 | 2253114 |
| circulation in italy of beta-lactamase-producing strains within the major groups of bacterial pathogens. | a multicenter study was undertaken in italy to assess the circulation of beta-lactamase-producing organisms and their current incidence within the major groups of bacterial pathogens. almost four thousand strains, all freshly isolated from clinical material, were examined at four centers serving different areas of italy. despite some significant center-to-center differences, this survey documented the occurrence of a large overall circulation of beta-lactamase-producing organisms among clinical ... | 1990 | 2253734 |
| lung cavities in patients with coeliac disease. | the clinical, radiological and pathological features of 7 patients with coeliac disease (cd) who developed lung abscesses or cavities are described. these patients were seen during a 20-year period during which time approximately 600 coeliacs were seen and 50 died. six of the coeliac patients with lung abscess died. the patients were middle aged. staphylococcal infection, klebsiella pneumoniae, bronchial carcinoma and previous pulmonary tuberculosis accounted for the cavities in 4 patients. in t ... | 1990 | 2253825 |
| cloning of the klebsiella aerogenes nac gene, which encodes a factor required for nitrogen regulation of the histidine utilization (hut) operons in salmonella typhimurium. | the nac (nitrogen assimilation control) gene from klebsiella aerogenes, cloned in a low-copy-number cloning vector, restored the ability of k. aerogenes nac mutants to activate histidase and repress glutamate dehydrogenase formation in response to nitrogen limitation and to limit the maximum expression of the nac promoter. when present in salmonella typhimurium, the k. aerogenes nac gene allowed the hut genes to be activated during nitrogen-limited growth. thus, the nac gene encodes a cytoplasmi ... | 1990 | 2254273 |
| regulation of assimilatory nitrate reductase formation in klebsiella aerogenes w70. | klebsiella aerogenes w70 could grow aerobically with nitrate or nitrite as the sole nitrogen source. the assimilatory nitrate reductase and nitrite reductase responsible for this ability required the presence of either nitrate or nitrite as an inducer, and both enzymes were repressed by ammonia. the repression by ammonia, which required the ntr (nitrogen regulatory) system (a. macaluso, e. a. best, and r. a. bender, j. bacteriol. 172:7249-7255, 1990), did not act solely at the level of inducer e ... | 1990 | 2254283 |
| catabolism of 3-hydroxybenzoate by the gentisate pathway in klebsiella pneumoniae m5a1. | growth of klebsiella pneumoniae m5a1 on 3-hydroxybenzoate leads to the induction of 3-hydroxybenzoate monooxygenase, 2,5-dihydroxybenzoate dioxygenase, maleylpyruvate isomerase and fumaryl-pyruvate hydrolase. growth in the presence of 2,5-dihydroxybenzoate also induces all of these enzymes including the 3-hydroxybenzoate monooxygenase which is not required for 2,5-dihydroxybenzoate catabolism. mutants defective in 3-hydroxybenzoate monooxygenase fail to grow on 3-hydroxybenzoate but grow normall ... | 1990 | 2256782 |
| hospital and catchment area antibiotic utilization and bacterial sensitivity in primary infections following gastric surgery in huddinge, sweden. | the relationship between hospital and catchment area utilization of antimicrobial agents and the antibiotic sensitivity of bacteria isolated from primary infections has been studied after 750 gastric operations performed between 1972 and 1986 at the huddinge university hospital. over 80% of the antibiotics were used in the catchment area. penicillins (g and v) overall were the most commonly used drugs and comprised 37% of the total antibiotic consumption in 1977-1986. narrow-spectrum antibiotics ... | 1990 | 2257854 |
| [cloning and expression of clostridium thermocellum f7 endoglucanase gene in gram-negative bacteria]. | the endoglucanase gene of clostridium thermocellum f7 was cloned in escherichia coli cells using pkm4 vector. both the physical mapping and analysis of the gene products in the e. coli mini-cells system suggest cloning of a new cel gene different from those described earlier. the activity of endoglucanase in e. coli cells is localized in the periplasm, which correlates with secretion of enzymes of this type in c. thermicellum. apart from 2 major components with mr 42.5 and 43 kda, corresponding ... | 1990 | 2258032 |
| pefloxacin efficacy in gram-negative bacillary meningitis. | sixteen patients with acute meningitis caused by gram-negative bacteria were treated with pefloxacin intravenously. the age range of the patient group was six months to 85 years with a mean age of 40 years; three patients were children. in all but two patients meningitis was a complication of neurosurgical operations and fourteen of the sixteen had received prior therapy which was not successful. the causative organisms were: pseudomonas aeruginosa (5), acinetobacter calcoaceticus (4), klebsiell ... | 1990 | 2258348 |
| pefloxacin in the treatment of septicaemia: three years' experience. | sixty cases of septicaemia treated with pefloxacin, alone (30) or in combination with other antibiotics (30) were analysed retrospectively. a satisfactory outcome was achieved in 33 (87%) of 38 evaluable gram-negative septicaemias (including success in 25 of 29 treated with pefloxacin alone) and in 15 of 21 staphylococcal septicaemias, mainly treated with combinations of antibiotics. failures and relapses were associated with a variety of local or systemic underlying conditions. | 1990 | 2258358 |
| trial and error in producing ankylosing-spondylitis-selective antisera according to andrew geczy. | geczy found that rabbit sera raised against klebsiella strain k43 cross-reacted with the cells from hla-b27 positive patients with ankylosing spondylitis (as). other laboratories failed to reproduce these results. after a series of unsuccessful attempts, however, we managed to prepare one selective antiserum, using e. coli, isolated from a dutch bechterew patient, in offspring of rabbits geczy sent us. ever since we obtained irreproducible results only. this paper reports about the many attempts ... | 1990 | 2259890 |
| attempt to show inhibition by oxygen in the modifying factor assay. | an australian laboratory has demonstrated that antibodies to certain strains of enteric bacteria react with cells from hla-b27-positive patients with a 'modifying factor' (mf) from these bacteria. these observations have proved difficult to reproduce. we investigated the possibility that oxygen inhibits the test by blocking b27, but we were unable to obtain consistent positive results. | 1990 | 2259891 |
| neonatal septicaemia in harare hospital: aetiology and risk factors. the puerperal sepsis study group. | during february to june 1987, one hundred and sixty one babies admitted to harare neonatal unit had positive blood cultures, giving a sepsis rate of 21/100 live births at harare hospital. the case records of these 161 babies were reviewed and compared with records of 50 babies admitted from the same catchment area and during the same time period but who had negative blood cultures. babies with early (less than 48 hr) onset or late onset sepsis tended to have lower birth weights and shorter gesta ... | 1990 | 2261631 |
| antibody coated bacteria in urine of patients with recent spinal injury. | twenty patients with an acute spinal injury were prospectively studied to assess the clinical importance of antibody coated bacteria (acb) in the urine and the association among the different bacterial species with a positive antibody coated bacteria test. clinical urinary tract infection was associated with a positive acb test on 45% of occasions. three hundred and ninety nine urine samples containing 541 bacterial isolates were assessed for the presence of acb; 13% were found to be positive an ... | 1990 | 2262569 |
| [susceptibilities of uropathogenic bacteria to ampicillin, cefazolin, cefmetazole and gentamicin. nine-year survey of changing patterns of susceptibilities]. | we analyzed antibiotic susceptibilities of urinary isolates of escherichia coli, klebsiella spp., proteus mirabilis, and indole (+) proteus group to ampicillin (abpc), cefazolin (cez), cefmetazole (cmz) and gentamicin (gm) in 69 laboratories in 1988 and also studied changing patterns of susceptibilities from 1980 to 1988. minimal inhibitory concentrations were determined using the agar dilution method (mueller-hinton agar, bbl) with inoculation of 10(6) cfu/ml of bacteria. ninety to 99% of the s ... | 1990 | 2262960 |
| [clinical laboratory approach to estimate effective administrative dose of minocyclin. reevaluation of in vitro mic break points in disc susceptibility test]. | antimicrobial activities of minocycline (mino) against various clinical isolates, 270 strains obtained in 1988, were determined and the reliability of the mino disc susceptibility test in estimating approximate values of mics was studied. clinical significance of a 4 category system for the interpretation of the disc tests, which is widely used in japan, and that of a 3 category system used in the usa and europe, were also evaluated to determine which system would be more suitable for the evalua ... | 1990 | 2262962 |
| in vitro effect of chlorhexidine and amikacin on oral gram-negative bacilli from bone marrow transplant recipients. | prophylactic use of chlorhexidine (chx) mouthrinses has been shown to benefit the oral health status of bone marrow transplant recipients and other immunosuppressed persons and to reduce systemic complications of oral origin. however, a problem that often emerges with these patients is oropharyngeal and lower respiratory tract colonization by opportunistic aerobic or facultative gram-negative bacilli (gnb). trends in four studies indicated that chx rinses may predispose these persons to oral col ... | 1990 | 2263328 |
| cefotaxime and aminoglycoside treatment of meningitis caused by gram-negative enteric organisms. | we reviewed cases of gram-negative enteric bacillary meningitis in infants and children treated with cefotaxime at texas children's hospital from january, 1984, through june, 1989. seventeen of 20 children had an underlying condition predisposing to the development of meningitis. the etiologic organisms in these 20 children (2 days to 12 years old; median, 12 days old) were klebsiella sp, 9; escherichia coli, 4; enterobacter cloacae, 3; citrobacter diversus, 2; other, 2. with the exception of on ... | 1990 | 2263430 |
| [the preventive treatment of recurring respiratory infections using ru 41740 in 3,008 children]. | biostim is an immunomodulating agent of biologic origin indicated for the prophylactic treatment of recurrent respiratory tract infections in adults and children over one year of age. it has been proved effective by double-blind placebo-controlled trials. this study was undertaken to evaluate the value and tolerance of biostim in a large number of patients under the conditions of actual use. three thousand and eight patients were included in the study. at inclusion, 93.7% of patients had a histo ... | 1990 | 2264700 |
| [experimental and clinical study of nitazol as an antibacterial drug in the complex treatment of peritonitis]. | antibacterial properties of the soviet drug nitazol which is a derivative of imidazole were studied. it was shown that nitazol in a dose of 4-8 micrograms/ml was highly active against gram-negative nonsporulating anaerobes, gram-positive anaerobic cocci and spore-forming clostridia spp. unlike metronidazole, it was efficient against both standard and clinical strains of facultative anaerobes such as e. coli, s. aureus and klebsiella spp. isolated from patients with peritonitis and being poly-res ... | 1990 | 2264753 |
| the nifen genes participating in femo cofactor biosynthesis and genes encoding dinitrogenase are part of the same operon in bradyrhizobium species. | the nucleotide sequences of genes homologous to the klebsiella pneumoniae nifen genes have been determined in bradyrhizobium japonicum 110. the coding regions for the nife and nifn consist, respectively, of 1641 and 1407 nucleotides. the nifd gene (coding for the beta-subunit of dinitrogenase) and nife are linked, and separated by 95 nucleotides. in the region of 12 nucleotides that separates nife from nifn the stop codon for nife overlaps the putative ribosome binding site for nifn. in contrast ... | 1990 | 2266945 |
| [clinico-immunological aspects of intestinal klebsiella infection]. | | 1990 | 2267596 |
| survival of bacteria during aerosolization. | one form of commercial application of microorganisms, including genetically engineered microorganisms is as an aerosol. to study the effect of aerosol-induced stress on bacterial survival, nonrecombinant spontaneous antibiotic-resistant mutants of four organisms, enterobacter cloacae, erwinia herbicola, klebsiella planticola, and pseudomonas syringae, were sprayed in separate experiments in a greenhouse. samples were collected over a distance of 15 m from the spray site for enumeration. spores o ... | 1990 | 2268156 |
| klebsiella pneumoniae nitrogenase. mechanism of acetylene reduction and its inhibition by carbon monoxide. | the electron flux through the mofe-protein of nitrogenase from klebsiella pneumoniae determines the absolute and relative rates of 2h+ reduction to h2 and acetylene (c2h2) reduction to ethylene (c2h4) at saturating levels of reductant (na2s2o4) and mgatp. high electron flux, induced by a high fe-protein (kp2)/mofe protein (kp1) ratio, favours c2h2 reduction. these data can be explained if ethylene, the two-electron reduction product of c2h2, is not released until three electrons have been transf ... | 1990 | 2268290 |
| treatment of community-acquired lower respiratory tract infections with oral cefuroxime axetil. | the subjects were adult hospitalized patients, 12 with pneumonia and eight with acute bronchitis. the patients with pneumonia received 500 mg of cefuroxime orally twice daily and the patients with bronchitis received 250 mg twice daily. treatment lasted for ten days in responsive patients. the pathogens identified in the patients' sputum were streptococcus pneumoniae, klebsiella pneumoniae, haemophilus influenzae, enterobacter aerogenes, staphylococcus aureus, or branhamella catarrhalis. clinica ... | 1990 | 2268866 |
| nonspecific resistance-enhancing activity of zymosan in experimental bacterial infections. | the nonspecific resistance-enhancing activity of zymosan on experimental bacterial (escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, serratia marcescens and staphylococcus aureus) infections was investigated. zymosan, being of very low toxicity, significantly enhanced nonspecific resistance to the above bacteria. the enhancement was the most pronounced 2 to 5 days after zymosan treatment. | 1990 | 2270737 |
| [etiology, diagnosis and treatment of hepatic abscesses after biliary tract surgery]. | the majority of hepatic abscesses of biliary tract origin are the result of suppurative or non suppurative cholangitis, caused either by common duct stones, inflammatory stricture or malignancies. we report our experience of 7 consecutive liver abscesses after biliary tract surgery, treated in our surgical unit over a 2-year period, there were 6 females and 1 male with an age of 41 to 83 years. hepatic abscess after biliary surgery developed from 10 days to 14 months postoperatively. two patient ... | 1990 | 2270919 |
| citrate substitutes for homocitrate in nitrogenase of a nifv mutant of klebsiella pneumoniae. | an organic acid extracted from purified dinitrogenase isolated from a nifv mutant of klebsiella pneumoniae has been identified as citric acid. h2 evolution by the citrate-containing dinitrogenase is partially inhibited by co, and by some substrates for nitrogenase. the response of maximum velocities to changes in ph for both the wild-type and the nifv- dinitrogenase was compared. no substantial differences between the enzymes were observed, but there are minor differences. both enzymes are stabl ... | 1990 | 2271541 |
| fimbrial hemagglutinins in klebsiella pneumoniae. | eleven klebsiella pneumoniae strains were isolated from urine specimens which were examined for their ability to produce hemagglutinins (has). bacteria were grown under various culture conditions. suspension of bacteria grown in broth or on phosphate-buffered nutrient agar (pba) were tested for agglutination in the presence and absence of 2% (w/v) d-mannose, on rockedtiles at 4 degrees c and ambient temperature with mangroup-o(m), fowl(f), ox(o), guinea-pig(g), horse (h), rabbit (r) and sheep (s ... | 1990 | 2273249 |
| [cefoperazone--possibility of safer treatment for infections in patients with chronic renal failure]. | cefoperazone was used in the treatment of infections in 13 cases of chronic renal failure. in 8 cases recurrent urinary tract infections were diagnosed, septicaemia in 3 cases, pneumonia in 2. cefoperazone 2-4 g daily was a very effective and well tolerated drug. only one therapeutic failure was noted in a patient with mixed urinary tract infection (strains of enterobacter cloacae and klebsiella pneumoniae). no side effects developed, and in patients in the phase of moderate renal failure some d ... | 1990 | 2275197 |
| multiple scleral abscesses with recurrent bacterial endophthalmitis eight months following cataract surgery. | scleral involvement in association with endophthalmitis is unusual. we report a case of recurrent bacterial endophthalmitis and multiple scleral abscesses occurring 8 months following cataract extraction. culture of pus from scleral abscesses first grew pseudomonas aeruginosa; and klebsiella pneumoniae on recurrence. the patient responded to intensive topical and systemic antibiotic therapy and recovered visual acuity of 20/60. | 1990 | 2275363 |
| overview of gram-negative sepsis. | an overview of gram-negative sepsis is presented, and the need for improved treatment for this condition is emphasized. the availability of new and more potent antimicrobial agents has not substantially altered the mortality from sepsis and septic shock. gram-negative infection, bacteremia, sepsis, and septic shock remain major clinical problems, particularly among hospitalized patients. the estimated incidence of gram-negative sepsis in the united states alone is 200,000 cases annually. the pre ... | 1990 | 2275477 |
| [spectrum of pathogens in hospital infections in onco-urologic patients and their sensitivity to antibacterial drugs]. | retrospective examination of case records and laboratory findings related to 155 patients discharged from the urological department of the all-union oncology research centre, academy of medical sciences of the ussr within a period of 3 months showed that 41.9 per cent of the oncourological patients had infectious processes requiring antibacterial therapy. among 80 infectious episodes 62 or 77.5 per cent were of intrahospital origin. there was a relationship of the frequency of the infections to ... | 1990 | 2275593 |
| [role of microbiological surveillance in the choice of antibacterial therapy for intestinal klebsiella infection in infants]. | data on the frequency of intestinal infections caused by klebsiella spp. in infants at the age of 1 month to 1 year and antibiotic resistance of k. pneumoniae and k. oxytoca isolates from newborns and infants aged 1 month to 1 year are presented. the frequency of the klebsiella isolates from the newborns and infants at the age of 1 month to 1 year with acute intestinal infections amounted to 17.7 +/- 1.4 and 42.5 +/- 1.4 per cent, respectively. the majority of the clinical strains had a multiple ... | 1990 | 2275594 |
| control of infection due to klebsiella pneumoniae in an intensive care nursery. | because of an increased incidence of necrotizing enterocolitis (nec) temporally related to klebsiella pneumoniae colonization noted in several infants, a study was undertaken to identify the source and to eradicate the infection. twenty infants in the neonatal intensive care unit (nicu) and 51 in the well-baby nursery were prospectively studied. cultures were done on all infants. in addition, cultures were done on all employees and parents who regularly visited. our results showed an increased i ... | 1990 | 2277281 |
| immunological cross-reactivity between enterobacter aerogenes and klebsiella capsular polysaccharides. | enterobacter aerogenes capsular polysaccharides (cps) which cross-react with klebsiella k3, k21, and k68 cps were purified. humans immunized with a vaccine containing klebsiella k3 and k21 cps produced antibody which recognized these e. aerogenes cps. the magnitude of the immune response to the e. aerogenes cps varied among the serotypes. passive transfer of an immunoglobulin produced from the plasma of donors immunized with the above vaccine afforded significant protection against challenge wit ... | 1990 | 2277587 |
| positive and negative regulation of expression of the l-sorbose (sor) operon by sorc in klebsiella pneumoniae. | in klebsiella pneumoniae the gene products involved in the degradation of the ketose l-sorbose are encoded in the sor operon. it comprises, besides structural genes for uptake and catabolism, a promoter-proximal gene sorc, encoding a protein sorc of mr 40 kda, for which no enzymatic function has been detected. all sor genes are coordinately expressed and inducible by l-sorbose. polar insertions and frameshift mutations in sorc cause a pleiotropic negative effect on the expression of all other so ... | 1990 | 2277638 |
| in vitro activity of hre 664, a penem antibiotic. | hre 664, a new penem antibiotic, inhibited 90% of escherichia coli, klebsiella, citrobacter diversus, proteus mirabilis, proteus vulgaris, salmonella, shigella, providencia, aeromonas, and morganella at less than or equal to 2 micrograms/ml but was considerably less active than cefotaxime, ceftazidime, and imipenem. it did not inhibit pseudomonas aeruginosa (mic greater than 128 micrograms/ml). hre 664 inhibited enterobacter spp., citrobacter freundii, and serratia marcescens at 1-8 micrograms/m ... | 1990 | 2279381 |
| bacterial isolates involved in cases of septicaemia in a nigerian hospital. | of the nine hundred and twenty patients clinically diagnosed of having septicaemia at the obafemi awolowo university teaching hospital complex, ile-ife, nigeria between 1980-1987, 233 (25.3%) had blood samples positive by culture. thirteen different bacterial species were identified from positive blood cultures of which six predominated: staphylococcus aureus (30.5%), coliforms (17.6%), klebsiella spp. (14.2%), pseudomonas aeruginosa (9.2%), escherichia coli (7.9%) and salmonella typhi (5.9%). t ... | 1990 | 2279469 |
| [successful treatment of chronic lung abscess by the method of operative selective occlusion of the bronchi]. | | 1990 | 2280543 |
| the influence of the klebsiella pneumoniae regulatory gene nifl upon the transcriptional activator protein nifa. | the influence of the klebsiella pneumoniae nifl gene product upon the interaction of the transcriptional activator protein nifa with the nifh promoter has been examined using in vivo dimethylsulphate 'footprinting'. binding of nifa to the upstream activator sequence (uas) of the nifh promoter in the presence of the nifl protein was observed under nitrogen-limiting growth conditions. growth in the presence of nh4+ or addition of nh4+ to nitrogen-limited cells diminished the interaction of nifa wi ... | 1990 | 2280685 |
| antibacterial effect of zinc oxide in vitro. | antibacterial activity, zinc concentrations and ph were measured in müller-hinton broth containing different amounts of zinc oxide and inoculated with staphylococcus aureus (10(6) colony forming units/ml). the minimum inhibitory concentrations (mic) of zinc oxide to different clinical isolates were determined using the müller-hinton agar dilution tests. gram-positive bacteria were most susceptible. gram-negative aerobic bacteria and streptococci were usually not inhibited even at the highest con ... | 1990 | 2281305 |
| antibacterial activity of rosin and resin acids in vitro. | the antibacterial effects of rosins and resin acids were studied in vitro using three methods, disc diffusion on agar, agar dilution, and broth dilution. rosin and some resin acids had antibacterial effects that were restricted to gram-positive bacteria. the abietic type of acids had a more pronounced antibacterial activity than the pimaric and labdane acids when the disc diffusion method was used but there was no inhibition of growth of gram-negative bacteria. among the individual resin acids, ... | 1990 | 2281306 |
| antibiotic resistance among coliform bacteria isolated from carcasses of commercially slaughtered chickens. | a total of 322 coliform bacteria escherichia coli, enterobacter spp., citrobacter spp., klebsiella spp. and serratia spp., were isolated from 50 carcasses of commercially slaughtered chickens. their resistance to ampicillin, tetracycline, gentamicin, chloramphenicol, cephalotine, cotrimoxazole, nalidixic acid and nitrofurantoin, were determined. the most commonly found resistance was to tetracycline followed by cephalotine, cotrimoxazole and nalidixic acid. a large percentage of e. coli (41%) an ... | 1990 | 2282290 |
| molecular cloning, expression and nucleotide sequence of the rcsa gene of erwinia amylovora, encoding a positive regulator of capsule expression: evidence for a family of related capsule activator proteins. | a gene encoding a positive activator of the expression of extracellular polysaccharide (eps) synthesis in the phytopathogen erwinia amylovora has been isolated from a genomic library in escherichia coli. the presence of the cloned gene in e. coli stimulated transcription of the genes encoding colanic acid biosynthesis and could complement rcsa mutations. introduction of the gene on a multicopy plasmid into er. amylovora caused a threefold increase in eps expression. the nucleotide sequence of th ... | 1990 | 2283503 |
| [primary splenic abscess in a brother and sister with thalassemia major]. | | 1990 | 2283787 |
| [comparative study of the effect of various antiseptics and ozone gas on pathogens of ulcus cruris]. | the germicidal influence of various antiseptics on the bacterial and fungal flora of leg ulcers was studied in vitro. best effects were achieved by the triphenylmethane dyes brilliant green and methylrosaniline chloride. chloramine and povidone iodine yielded average results, whereas the germicidal effects of potassium permanganate and eosin were not satisfactory. on the other hand, treatment with ozone resulted in sufficient inactivation of germs--with the only exceptions of serratia and klebsi ... | 1990 | 2284834 |
| nucleotide sequence and phylogeny of shv-2 beta-lactamase. | we determined the nucleotide sequence of the blashv-2(pbp60-1) gene from klebsiella ozaenae which confers resistance to broad-spectrum cephalosporins. the structural gene encodes a polypeptide product of 286 amino acids, and the estimated molecular weight of the mature protein is 28,900. amino acid sequence comparison of the shv-2pbp60-1 enzyme with all known class a beta-lactamases and homology studies showed that the residues were highly conserved. furthermore, shv-2pbp60-1 was clearly related ... | 1990 | 2285285 |
| in vitro activities of 15 oral beta-lactams against klebsiella pneumoniae harboring new extended-spectrum beta-lactamases. | the activities of 15 oral beta-lactams against klebsiella pneumoniae harboring new extended-spectrum beta-lactamases were studied. all compounds were affected by these enzymes, especially by the shv derivatives. except for ceftibuten, the compounds with the greatest intrinsic activity were more affected by the presence of these enzymes than were older compounds with moderate intrinsic activity. | 1990 | 2285291 |
| [neonatal meningitis: observations on its etiology, mortality and sequelae]. | a retrospective study was carried out on all cases diagnosed with neonatal meningitis at the hospital infantil de méxico federico gómez. a rate of 9.6 cases per 1,000 discharged patients was found as well as 6.7% association with sepsis. in 50 of the cases analyzed, an etiologic agent was identified in 23 children; the main bacteria identified were escherichia coli (24%), klebsiella pneumoniae (14%), enterobacter (4%), proteus mirabilis and pseudomonas sp (2%), respectively. the mortality rate w ... | 1990 | 2285466 |
| the role of microorganisms in psoriasis. | the microflora of 297 psoriasis patients was extensively examined. throat, urine, and skin surfaces from scalp, ears, chest, face, axillary, submammary, umbilical, upper back, inguinal crease, gluteal-fold, perirectal, vaginal, pubis, penis, scrotal, leg, hands, feet, finger, and toenail areas were cultured for aerobic bacteria, yeast, and dermatophytes. antibody levels to streptococcal enzymes were performed (streptolysin-o, dnase-b, hyaluronidase, streptozyme). giemsa smears and koh preparatio ... | 1990 | 2285571 |
| clinico-bacteriological study of neonatal conjunctivitis. | perinatal risk factors and bacteriological profile of conjunctivitis were studied among 245 newborns delivered at jipmer hospital between july 1986 and june 1988. maternal and neonatal factors like lack of antenatal care, presence of adverse intrapartum factors, operative deliveries, birth asphyxia, pre-maturity and prolonged hospital stay significantly increased the chances of developing conjunctivitis (p less than 0.001). bacteria were recovered from 163 (66.5%) cases. staphylococcus was the c ... | 1990 | 2286405 |
| severe infections treated with intravenous ofloxacin: a prospective clinical multicentre swiss study. | in this open prospective clinical trial of iv ofloxacin, 87 patients were treated for severe bacterial infections. overall results showed a clinical cure rate of 75% and 79/87 87 patients improved. 86% of causative organisms (all sensitive to ofloxacin) were eliminated. the best results were obtained in urinary tract infections where all 30 patients were clinically cured and 27 organisms (escherichia coli, group d streptococcus, proteus mirabilis and klebsiella pneumoniae) were eliminated. rapid ... | 1990 | 2286585 |
| efficacy of intravenous ofloxacin: a french multicentre trial in 185 patients. | the efficacy of intravenous ofloxacin therapy (200 mg 12-hourly) followed, when appropriate, by oral administration of the same dose was evaluated in an open multicentre trial involving 185 patients in 31 french hospitals. dosage adjustment was made for patients in renal failure. infection was hospital-acquired in 35 cases, 53 patients required admission to an intensive care unit. the infections comprised septicaemia (n = 56), pneumonia (n = 18), bronchitis (n = 10), urinary tract (n = 78), fema ... | 1990 | 2286586 |
| intravenous ofloxacin in severe infections. | the efficacy and tolerance of intravenous ofloxacin was studied in 70 patients suffering from soft tissue infections (n = 33), intra-abdominal abscesses (n = 14), septicaemia (n = 12), pneumonia (n = 9) and brucellosis (n = 2). the average daily dose was 6 mg/kg divided into two doses. pathogens treated included enterobacter cloacae (n = 14), escherichia coli (n = 12), staphylococcus aureus (n = 13), pseudomonas aeruginosa (n = 11), klebsiella pneumoniae (n = 10), enterococcus faecalis (n = 8) a ... | 1990 | 2286587 |
| [enzyme-linked immunosorbent assay (elisa) for legionella pneumophila using 60-kda protein antigen]. | the enzyme-linked immunosorbent assay (elisa) has been evaluated for the detection of antibodies against legionella pneumophila. three-grade antigens were prepared from legionella pneumophila serogroup i. crude antigen was made by enzyme digestion, sonication and centrifugation and then became half pure by ammonium sulfate precipitation. it was purified to form 60-kda protein antigen by size-exclusion chromatography on a sephacryl s400 column and ion-exchanged chromatography on a deae-5pw column ... | 1990 | 2286787 |
| [clinical study of s 6472 in urinary tract infection]. | clinical studies on s 6472, a longer lasting preparation of cefaclor (ccl), were performed and the following results were obtained. s 6472 was administered orally to 102 patients with urinary tract infections including 16 with acute uncomplicated pyelonephritis, 32 with acute uncomplicated cystitis, 31 with complicated pyelonephritis and 23 with complicated cystitis. 95 patients were treated with 375 mg of s 6472 2 times daily and 7 patients were treated with 750 mg of s 6472 2 times daily. the ... | 1990 | 2287054 |
| [susceptibility of clinically isolated bacterial strains to imipenem/cilastatin sodium]. | in vitro antibacterial activities of imipenem/cilastatin sodium (imipenem) and other beta-lactams against clinically isolated 353 bacterial strains were investigated. the results obtained in this study are summarized as follows: 1. imipenem (ipm) showed potent antibacterial activities against gram-positive cocci such as staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, streptococcus agalactiae. 2. ipm had inferior or equivalent antibacterial activities to beta-lactams aga ... | 1990 | 2287059 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates]. | this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ... | 1990 | 2287060 |
| analysis of site-directed mutations in the alpha- and beta-subunits of klebsiella pneumoniae nitrogenase. | using directed mutagenesis, amino acid substitutions have been made in the alpha- and beta-subunits of the klebsiella pneumoniae nitrogenase component 1 at positions normally occupied by conserved cysteine or tyrosine residues. nif+, nif- and intermediate phenotypes have been obtained. to extend our earlier biochemical characterization (kent et al., 1989) the electrophoretic mobility of component 1 of the mutant and wild-type nitrogenases has been analysed by non-denaturing gel electrophoresis. ... | 1990 | 2287274 |
| cloning and physical mapping of the sor genes for l-sorbose transport and metabolism from klebsiella pneumoniae. | the sor genes of klebsiella pneumoniae kay2026, which enable the bacterium to metabolize the ketose l-sorbose, have been cloned on an 8.3kb dna fragment into the multicopy plasmid, pacyc184. the genes were mapped by restriction analysis, by deletion mapping and by insertion mutagenesis with tn1725. the corresponding gene products were identified by the maxicell technique. the structural genes sord, sora and sore code for a d-glucitol-6-p dehydrogenase (27 kilodalton (kd)), an enzymell (ellsor) a ... | 1990 | 2287279 |
| use of plasmid analysis to determine the source of bacterial invasion of the urinary tract. | gram negative colonisation and infection of the urinary tract is a well recognised complication of the neuropathic bladder caused by spinal cord injury (sci). k. pneumoniae accounts for one third of all urinary tract infections in hospitalised sci patients. plasmid analysis has been shown to reliably fingerprint bacterial strains, particularly k. pneumoniae, so that growth from two separate locations in or on the body can be accurately analysed as to migration from a reservoir to a target locati ... | 1990 | 2287522 |
| [endogenous bacterial endophthalmitis. report of 3 cases]. | since the antibiotic era, endophthalmitis has been a rare complication of bacterial septicemia. the authors report hereby 3 cases of endogenous bacterial endophthalmitis. both eyes were involved in one case. causative bacteria were identified in blood. cultures were positive respectively for streptococcus pneumoniae, staphylococcus aureus and klebsiella pneumoniae. all patients received intravenous antibiotherapy, once intravitreal antibiotic was injected. clinical manifestations were three time ... | 1990 | 2290003 |
| [inhalation therapy of antibiotics]. | we produced experimental murine klebsiella pneumoniae pneumonia by air-borne infection using exposure apparatus (ld50: 9.7 x 10 c.f.u./lung). the mic value of cefazolin was 1.56 micrograms/ml, and that of gentamicin was 0.39 micrograms/ml. we treated this murine pneumonia with aerosolized antibiotics. the infected mice received inhalation of 50 mg (5 mg/ml), 100 mg (10 mg/ml), 200 mg (20 mg/ml) and 400 mg (40 mg/ml) gentamicin were alive. survival rate of the infected mice treated with inhalatio ... | 1990 | 2290226 |
| bacteria causing respiratory infections in children and their sensitivity to antibiotics. | the author lists the most frequent bacterial agents isolated from the nose and pharynx samples of children that visited the child health centre from 1983 to 1986. the gram-positive bacteria found most often were: staphylococcus aureus (44% in 1983, 40% in 1986). streptococcus pneumoniae (6% in 1983, 12% in 1986), and streptococcus haemolyticus of group "a" (14% in 1983, 19% in 1986). the isolated gram-negative bacteria were: e. coli (14%), klebsiella enterobacter (8%), and proteus species and ps ... | 1990 | 2291383 |
| in vitro activity against clinically important gram-positive and gram-negative bacteria of sulbactam, alone and in combination with ampicillin, cefotaxime, mezlocillin, and piperacillin. | the in vitro effects of the single agents, and the synergistic/antagonistic action of three different combinations of ampicillin (amp, cas 69-53-4), cefotaxime (ctx, cas 63527-52-6), mezlocillin (mez, cas 51481-65-3), and piperacillin (pip, cas 61477-96-1) with the beta-lactamase inhibitor sulbactam (sul, cas 68373-14-8) were determined against 675 gram-positive and gram-negative, both aerobic and anaerobic bacteria. all the combinations of sulbactam and the antibiotics (1: 1, 1:2 and 1:4) exhib ... | 1990 | 2291754 |
| in-vitro activity of cefpodoxime against 1834 isolates from domiciliary infections at 20 uk centres. | a total of 1834 non-copy, general practice or outpatient isolates were collected by 20 hospital laboratories within the british isles, and identified and tested for susceptibility to nine antimicrobial agents available by oral administration at one centre. against enterobacteriaceae cefpodoxime was the most active beta-lactam agent tested, the mic90s being: for escherichia coli 1.0 mg/l, for proteus mirabilis 1.0 mg/l, for citrobacter spp. 2.0 mg/l, and for enterobacter spp., serratia spp., morg ... | 1990 | 2292533 |
| changes in the antibiotic sensitivities of urinary pathogens, 1971-1989. | all urinary pathogens from general practice and hospital have been tested for sensitivity to a range of antimicrobial agents for the last 19 years. there have been marked changes during that time. in general practice there has been a considerable increase in the proportion of staphylococcal infections from 5.1% to a peak of 14.8% in 1982 and a more recent decline to 3.4%. there has also been a decrease in the proportion caused by proteus mirabilis, from 9.2% to 5.0%. similar, but smaller changes ... | 1990 | 2292543 |
| handfishing-associated klebsiella bloodstream infection. | | 1990 | 2295851 |
| evolution of aromatic amino acid biosynthesis and application to the fine-tuned phylogenetic positioning of enteric bacteria. | a comprehensive phylogenetic tree for virtually the entire assemblage of enteric bacteria is presented. character states of aromatic amino acid biosynthesis are used as criteria, and the results are compared with partial trees based upon sequencing of 16s rrna, 5s rrna, and tryptophan leader peptide. three major clusters are apparent. enterocluster 1 possesses a gene fusion (trpg-trpd) encoding anthranilate synthase: anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase of tryptop ... | 1990 | 2298692 |
| influence of ru 41.740 on human monocytes in vitro: release of soluble factors which retard multiplication of tumor cells in culture. | ru 41.740 (biostim) is an immunostimulating substance of bacterial origin with a reported protective activity against bacterial infections in man. the aim of the present investigation was to determine if biostim-exposed blood mononuclear cells release soluble factors in vitro which may inhibit growth of tumor cells in culture. it was observed that exposure of mononuclear cells to biostim at concentrations as low as 1 ng/ml for 24 h resulted in the release of factors capable of retarding growth o ... | 1990 | 2303322 |
| amoxicillin-clavulanic acid therapy of spontaneous bacterial peritonitis: a prospective study of twenty-seven cases in cirrhotic patients. | spontaneous bacterial peritonitis in cirrhosis is a serious complication that demands urgent attention. we report here a prospective study of the treatment of 27 episodes of spontaneous bacterial peritonitis in 22 cirrhotic patients with amoxicillin and clavulanic acid. the infection of ascitic fluid was diagnosed by a positive culture plus an ascitic neutrophil count exceeding 75/microliters, or by an ascitic neutrophil count exceeding 500/microliters. the infection was treated with 1 gm amoxic ... | 1990 | 2312050 |
| [emphysematous pyelonephritis]. | the authors report on a case of a emphysematous pyelonephritis due to klebsiella pneumoniae, occurring in a diabetic patient and successfully treated by nephrectomy. they compare it to the literature. | 1990 | 2313112 |
| postantibiotic effect of the penem fce 22101 against selected gram-positive and gram-negative bacteria in vitro and in vivo by the use of a tissue cage model in rabbits. | isolates of staphylococcus aureus, streptococcus pneumoniae, haemophilus influenzae and klebsiella pneumoniae were tested for their bactericidal activity and postantibiotic effect (pae) with the new penem fce 22101. the tissue cage model in rabbits was used to study pae in vivo. the bactericidal activity against all four species was shown to be in the range of 0.05-4.0 mg/l. a 99.9% killing effect at mbc concentrations was reached within 2 hours with s. pneumoniae and k. pneumoniae and within 6- ... | 1990 | 2317348 |
| prevention of fatal infections by recombinant human interleukin 1 alpha in normal and anticancer drug-treated mice. | the preventive capability of interleukin 1 alpha (il-1) against bacterial infections was estimated in normal and anticancer drug-treated balb/c mice in comparison with ok432, granulocyte colony-stimulating factor, interferons alpha and gamma, and interleukin 2. pretreatment with il-1 (days -4 and -2) resulted in a significantly higher survival rate in normal mice inoculated i.p. with klebsiella pneumoniae, pseudomonas aeruginosa or listeria monocytogenes (day 0). the i.p. and s.c. administration ... | 1990 | 2317799 |
| purification and n-terminal analysis of urease from helicobacter pylori. | urease of helicobacter pylori (formerly campylobacter pylori) is believed to represent a critical virulence determinant for this species. ammonia generated by hydrolysis of urea may protect the acid-sensitive bacterium as it colonizes human gastric mucosa. an h. pylori strain, cultured from a gastric biopsy of a patient with complaints of abdominal pain and a history of peptic ulcer disease, was isolated on selective medium and cultured in mueller-hinton broth supplemented with 4% fetal calf ser ... | 1990 | 2318539 |
| efficiency factors and atp/adp ratios in nitrogen-fixing bacillus polymyxa and bacillus azotofixans. | the efficiency factor, the number of moles of atp generated per mole of glucose fermented, was determined in anaerobic, non-carbon-limited n2-fixing cultures of bacillus polymyxa, bacillus macerans, bacillus azotofixans, and clostridium butyricum through identification and quantitation of the fermentation products by 13c nuclear magnetic resonance spectroscopy and measurement of acetate kinase activities. all three bacillus species had acetate kinase activities and produced acetate and ethanol a ... | 1990 | 2318806 |
| emphysematous cystitis: a complication of urinary tract infection occurring predominantly in diabetic women. | the case of a patient with emphysematous cystitis who presented with complaints of lower abdominal pain, dysuria, and pneumaturia is presented. the presenting symptoms, differential diagnosis, and radiographic and cystoscopic appearances of emphysematous cystitis, a rare complication of lower urinary tract infection occurring almost exclusively in diabetics, are reviewed. treatment consists of urinary drainage, prompt initiation of antibiotic therapy, and strict glucose control. the prognosis us ... | 1990 | 2321826 |
| [the pathologic anatomy of important forms of bacterial pneumonia]. | postmortem examinations were conducted on 7,952 cases with acute inflammatory processes of respiratory organs, among them 452 adults, between 1949 and 1988. bacteria had been responsible for 87.5%, of these pneumonia cases, including klebsiella for 15.3%, pseudomonas for 13.5%, staphylococci for 9.9%, pneumococci for 4%, streptococci for 3.9% and haemophilus for 0.7%. other bacterial species of lower pathogenicity were recorded from 24.4% of all individuals who had died with pneumonia and mixed ... | 1990 | 2327186 |
| in vivo selection for transmissible drug resistance in salmonella typhi during antimicrobial therapy. | we report the recovery of salmonella typhi that acquired resistance to ampicillin, chloramphenicol, trimethoprim-sulfamethoxazole, and gentamicin subsequent to multiple antibiotic therapy. escherichia coli and klebsiella pneumoniae isolates which were recovered from the same stool sample displayed identical resistance patterns. agarose gel electrophoresis revealed that s. typhi and laboratory-derived transconjugants contained a high-molecular-weight plasmid present in the resistant intestinal ba ... | 1990 | 2327754 |
| in vitro activity of ro 23-9424, a dual-action cephalosporin, compared with activities of other antibiotics. | the in vitro activity of ro 23-9424, which is desacetyl-cefotaxime linked to fleroxacin, was compared with the activities of cefotaxime, desacetyl-cefotaxime, fleroxacin, ofloxacin, and ciprofloxacin. it inhibited the majority of members of the family enterobacteriaceae, except for some serratia marcescens, citrobacter freundii, and enterobacter cloacae strains, at less than or equal to 0.25 microgram/ml and had an mic for 90% of strains tested (mic90) of 8 micrograms/ml against pseudomonas aeru ... | 1990 | 2327765 |
| molecular epidemiology of tem-3 (ctx-1) beta-lactamase. | a total of 33 clinical isolates encoding tem-3 (ctx-1) from four french hospitals were studied. the strains belonged to seven species, klebsiella pneumoniae (n = 24), escherichia coli (n = 3), serratia marcescens (n = 2), citrobacter freundii (n = 1), enterobacter aerogenes (n = 1), enterobacter cloacae (n = 1), and klebsiella oxytoca (n = 1). all the strains harbored an inc7 or m self-transferable plasmid with a size of approximately 85 kilobases. the plasmids had closely related ecori, hincii, ... | 1990 | 2327769 |
| iron k-edge x-ray-absorption spectroscopy of the iron-vanadium cofactor of the vanadium nitrogenase from azotobacter chroococcum. | iron k-edge e.x.a.f.s. data for the iron-vanadium cofactor (fevaco) from azotobacter chroococcum vanadium nitrogenase reported here provide further evidence for the structural similarity between this and the iron-molybdenum nitrogenase cofactor (femoco) from klebsiella pneumoniae molybdenum nitrogenase [arber, flood, garner, gormal, hasnain & smith (1988) biochem. j. 252, 421-425]. the e.x.a.f.s. data are consistent with the vanadium being present in a v-fe-s cluster, thus confirming that the n- ... | 1990 | 2327976 |
| the in-vitro activity of ceftibuten against 475 clinical isolates of gram-negative bacilli, compared with cefuroxime and cefadroxil. | the in-vitro activity of ceftibuten was compared with cefuroxime and cefadroxil against 475 clinically-significant, epidemiologically-distinct isolates of gram-negative bacilli: 170 from blood, 212 from urine and 93 from a supplementary collection of multiply-resistant strains known to have resistance plasmids, to have caused sporadic or epidemic nosocomial infection, or both. ceftibuten mics ranged from 0.003 to greater than 32 mg/l, with a modal mic of 0.01 mg/l: 95% of all isolates had ceftib ... | 1990 | 2329100 |
| nosocomial septicemia in the cancer patient: the influence of central venous access devices, neutropenia, and type of malignancy. | nosocomial septicemias that occurred over a 32-month period on an inpatient medical oncology service were reviewed. one hundred four episodes of septicemia occurred in 84 patients, 33% with solid tumors and 67% with leukemia or lymphoma. sixty were primary septicemias, with the remainder being secondary. of the 118 isolates recovered, 42% were gram-positive organisms, 45% gram-negative organisms, and 13% were fungi. coagulase-negative staphylococci and escherichia coli were the most common gram- ... | 1990 | 2329966 |
| epidemiology of antibiotic resistance in human isolates of enterobacteriaceae in sicily. | the authors have studied the antimicrobial susceptibility of 1073 clinical isolates of various genera of enterobacteriaceae (collected during the period july-december 1988) to ampicillin, piperacillin, cefotaxime, ceftazidime, ceftriaxone, aztreonam, imipenem, gentamicin, amikacin, netilmicin, norfloxacin, and ciprofloxacin. antimicrobial susceptibility was determined by bauer-kirby disk diffusion method. of 1073 tested bacteria, 704 (65.6%) produced beta-lactamase detectable by nitrocefin test. ... | 1990 | 2332782 |
| [microflora of suppurative inflammatory foci in surgical patients and its sensitivity to antibiotics]. | materials from 1134 patients with various surgical infections were tested. from the materials collected from purulent inflammatory foci 2137 cultures were isolated. many of them belonged to micrococcaceae (63.7 per cent). cultures belonging to enterobacteriaceae and streptococcaceae were isolated from 50.9 and 25.2 per cent of the patients respectively. isolates belonging to other families were less frequent: diphtheroids were isolated from 13.3 per cent of the patients and pseudomonas spp. were ... | 1990 | 2334244 |
| [studies on respiratory infections in primary care clinic (ii). distribution and antibiotic sensitivity to 45 agents of bacteria isolated from patients with respiratory infections visiting a doctor in private practice]. | the bacteriology of the isolates from the sputum or the throat swab of patients with respiratory infections visiting a doctor in private practice in sendai city during the period from march in 1988 to february in 1989 was documented, and their sensitivity to 45 antimicrobial agents was determined. of the 568 patients, 514 cases had acute pharyngitis, 8 cases each had acute tonsillitis and acute bronchitis, 7 cases were acute pneumonia, 6 cases had herpangina, 18 cases had hand-foot-mouth disease ... | 1990 | 2335751 |
| in vitro and in vivo activities of a new quinolone, win 57273, possessing potent activity against gram-positive bacteria. | the antibacterial activity of a new 7-dimethylpyridinyl quinolone, win 57273, was assessed by using in vitro and in vivo models. agar inclusion and broth dilution in vitro tests revealed broad-spectrum activity against gram-positive and selected gram-negative organisms, with the greatest potency observed against the staphylococci. the mic for 90% of coagulase-positive strains tested (mic90) was less than or equal to 0.002 micrograms/ml; for the coagulase-negative strains the mic90 was 0.008 micr ... | 1990 | 2344163 |
| beta-lactamase production in members of the family enterobacteriaceae and resistance to beta-lactam-enzyme inhibitor combinations. | recent reports that members of the family enterobacteriaceae that produce high levels of certain beta-lactamases are often resistant to ticarcillin-clavulanate prompted this study to assess the relationship between type and amount of enzyme produced and susceptibility to ticarcillin-clavulanate, piperacillin-tazobactam, and cefoperazone-sulbactam. agar dilution mics were determined by using 73 strains of enterobacteriaceae that produced a single beta-lactamase that had been characterized and qua ... | 1990 | 2344169 |
| synthesis and antimicrobial evaluation of some arylhydrazones of 4-[(2-methylimidazo[1,2-a]pyridine-3-yl)azo]benzoic acid hydrazide. | a short series of arylhydrazones of 4-[(2-methylimidazo[1,2-a]pyridine-3-yl)azo]benzoic acid hydrazide was synthesized and tested for antimicrobial activity. all of the compounds show antimicrobial activity against escherichia coli. a few members were also active against klebsiella pneumoniae, staphylococcus aureus and pseudomonas aeruginosa. the interplanar angle (theta) between the aryl ring and the adjacent azomethine group of some representative compounds was measured by electronic absorptio ... | 1990 | 2344260 |
| cytotoxic component(s) of klebsiella oxytoca on hep-2 cells. | a cytotoxic component(s) was detected in culture filtrates of klebsiella oxytoca isolated from patients with antibiotic-associated hemorrhagic colitis. eleven of 12 isolates exhibited cytotoxicity on hep-2 cells. the cytotoxic activity of k. oxytoca strain mh43-1 was stable for the treatment of 60 c for 30 min, but inactivated by the treatment of 100 c for 15 min. this cytotoxicity was not destroyed by the treatment with trypsin or pronase, and the component was filtrable through a membrane filt ... | 1990 | 2345532 |
| [neonatal septicemia: differences in full-term and pre-term newborn infants]. | neonatal septicemia was assessed by blood cultures in 115 newborns (nb) during a two years study in a pediatric hospital of reference in mexico city. the studied patients were divided in two groups of gestational age, and the differences of etiologic agents, clinical signs, laboratory findings and clinical outcome were compared at term and preterm neonates. we observed staphylococcus epidermidis became the first cause of septicemia in at term nb (p less than 0.001), while escherichia coli and kl ... | 1990 | 2346609 |
| synthesis of the tetrasaccharide repeating-unit of the polysaccharide from klebsiella type 23. | methyl 2-o-allyl-4-o-benzyl-3-o-(2,3,4,6-tetra-o-acetyl-beta-d- glucopyranosyl)-alpha-l-rhamnospyranoside was obtained by condensing methyl 2-o-allyl-4-o-benzyl-alpha-l-rhamnopyranoside with tetra-o-acetyl-alpha-d-glucopyranosyl bromide. benzylation, removal of the allyl group, and condensation of the product with ethyl 6-o-acetyl-2,3,4-tri-o-benzyl-1-thio-beta-d-glucopyranoside gave methyl 2-o-(6-o-acetyl-2,3,4-tri-o-benzyl-alpha-d-glucopyranosyl)-4-o-benzyl-3- o- (2,3,4,6-tetra-o-benzyl-beta-d ... | 1990 | 2346945 |
| a new method for induction of experimental autoimmune uveoretinitis (eau) in mice. | experimental autoimmune uveoretinitis (eau) was induced in two strains of mice by repeated-immunization protocol. sma mice (h-2 nondefined) and c57bl/6 mice (h-2b) were immunized with s-antigen mixed with klebsiella 03 lipopolysaccharide (k03 lps) repeatedly at intervals of 1 to 4 weeks. following the tertiary immunization, the mice exhibited histopathological changes of eau as well as significant immune responses to the antigen. the antigen doses required for successful eau induction were 4 mic ... | 1990 | 2347201 |
| septicemia in the elderly: incidence, etiology and prognostic factors. | a study on septicemia in the elderly (mean age 80.3 +/- 9.1 years) was carried out during 1982-85. a total of 184 episodes of bacteremia occurred in 175 patients (incidence rate 7.2%); 61% were attributed to community-acquired sepsis. gram-negative microorganisms accounted for 64% of all episodes and gram-positive for 30%. the overall mortality was 18.3%. using univariate analysis, significant factors associated with a high mortality were: hospital-acquired sepsis, respiratory infections as sour ... | 1990 | 2347685 |