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determination of mitochondrial cytochrome b gene sequence for red deer (cervus elaphus) and the differentiation of closely related deer meats.the cytochrome b gene sequence for red deer was determined using the dye terminator cycle sequencing method and used for identification of deer meat in meat and meat products. red deer showed a similarity of 94.1, 84.0, 81.1, 85.5 and 85.6% to sika deer (cervus nippon), bovine, pigs, sheep and goats, respectively. to differentiate the deer meat, oligonucleotide primers rd-1(5'-tcatcgcagcactcgctatagtacact-3'), rd-2(5'-atctccaagtaggtctggtgcgaataa-3') were designed for the region of the cytochrome ...199822060620
both mitogen-activated protein kinase (mapk)/extracellular-signal-regulated kinases (erk) 1/2 and phosphatidylinositide-3-oh kinase (pi3k)/akt pathways regulate activation of e-twenty-six (ets)-like transcription factor 1 (elk-1) in u138 glioblastoma cells.epidermal growth factor (egf) and its receptor (egfr) have been shown to play a significant role in the pathogenesis of glioblastoma. in our study, the egfr was stimulated with egf in human u138 glioblastoma cells. we show that the activated mitogen-activated protein kinase (mapk)/extracellular-signal-regulated kinases (erk) 1/2 pathway phosphorylated the e twenty-six (ets)-like transcription factor 1 (elk-1) mainly at serine 383 residue. mitogen-activated protein kinase kinase (mek) 1/2 inhibit ...201122085529
experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle: comparison to bovine spongiform encephalopathy in cattle.prion diseases or transmissible spongiform encephalopathies (tses) of animals include scrapie of sheep and goats; transmissible mink encephalopathy (tme); chronic wasting disease (cwd) of deer, elk and moose; and bovine spongiform encephalopathy (bse) of cattle. the emergence of bse and its spread to human beings in the form of variant creutzfeldt-jakob disease (vcjd) resulted in interest in susceptibility of cattle to cwd, tme and scrapie. experimental cross-species transmission of tse agents p ...201121908269
Vaccination with BM86, subolesin and akirin protective antigens for the control of tick infestations in white tailed deer and red deer.Red deer (Cervus elaphus) and white-tailed deer (Odocoileus virginianus) are hosts for different tick species and tick-borne pathogens and play a role in tick dispersal and maintenance in some regions. These factors stress the importance of controlling tick infestations in deer and several methods such as culling and acaricide treatment have been used. Tick vaccines are a cost-effective alternative for tick control that reduced cattle tick infestations and tick-borne pathogens prevalence while r ...201222079077
Modification to histopathological lesion severity score in red deer (Cervus elaphus) affected by Johne's disease. 201121851306
t(X;14)(p11.4;q32.33) is recurrent in marginal zone lymphoma and upregulates GPR34.Genetic events underlying pathogenesis of nodal and extranodal marginal zone lymphoma (MZL) are incompletely understood. We report here a novel t(X;14)(p11.4;q32.33) identified in 4 lymphoma cases: 2 with a mucosa-associated lymphoid tissue (MALT) lymphoma, 1 with a nodal MZL and 1 with gastric diffuse large B-cell lymphoma. In all cases lymphoma evolved from a previous auto-immune disorder. Fluorescence in situ hybridization (FISH) and molecular studies showed that t(X;14) which is mediated by ...201122058210
pronounced reduction of fluoride exposure in free-ranging deer in north bohemia (czech republic) as indicated by the biomarkers skeletal fluoride content and dental fluorosis.wild deer have been recommended as bioindicators of fluoride pollution. we compared bone fluoride concentrations and prevalence and severity of dental fluorosis in free-ranging european roe deer (capreolus capreolus) and red deer (cervus elaphus) from five counties in the northwestern part of the czech republic that had been collected by hunters and whose mandibles were presented at trophy exhibitions in the years 1996/1997 ("early period") and 2009 ("late period"). data on atmospheric fluoride ...201122137477
effect of vaccination and natural infection with mycobacterium avium subsp. paratuberculosis on specificity of diagnostic tests for bovine tuberculosis in farmed red deer (cervus elaphus).to assess the effect of vaccination against paratuberculosis (johne's disease) on reactivity to diagnostic tests for bovine tuberculosis (tb) in deer exposed to natural challenge with mycobacterium avium subsp. paratuberculosis (map), and to investigate map infection as a factor in tb test cross-reactivity at the herd level.201121851298
nitrogen metabolism of four raw meat diets in domestic cats.little nutritional information has been collected from domestic cats fed raw meat diets. the objective of the present study was to evaluate differences in n metabolism of domestic cats fed raw beef-based diet (66 % crude protein (cp) and 20 % fat), bison-based diet (49 % cp and 39 % fat), elk-based diet (79 % cp and 6 % fat) and horse-based diet (60 % cp and 26 % fat). a total of eight intact adult female cats were fed to maintain body weight in a cross-over design. daily food intake, faecal and ...201122005421
organochlorine compounds in red deer (cervus elaphus l.) and fallow deer (dama dama l.) from inland and coastal croatia.polychlorinated biphenyl (pcb) and organochlorine pesticide (ocp) levels in fat tissue of red and fallow deer (cervus elaphus l. and dama dama l.) from two inland and an adriatic area were established. of 17 analysed pcbs, pcb-28, pcb-138, pcb-153, pcb-180 and pcb-118 were found in all samples, whilst pcb-101 and pcb-170 were found in more than 50% of samples. they ranged between 0.03 and 5.98 ng g(-1) fat weight. of seven analysed ocps, hcb, γ-hch and 1,1-dichloro-2,2-di(4-chlorophenyl)ethylene ...201121904863
immediate-early transcriptional response to angiotensin ii in human adrenocortical cells.angiotensin ii binds to the angiotensin ii receptors type 1 (at1 receptors) in adrenocortical cells and triggers an intracellular signaling cascade leading to changes in the gene expression pattern. here, we show that stimulation with angiotensin ii induces the expression of biologically active early growth response (egr)-1, a zinc finger transcription factor, in human h295r adrenocortical cells. expression of a dominant-negative mutant of the ternary complex factor elk-1, a key transcriptional ...201121914770
suspicion of mycobacterium avium subsp. paratuberculosis transmission between cattle and wild-living red deer (cervus elaphus) by multi target genotyping.for epidemiological tracing of paratuberculosis (johne's disease), multi-target genotyping of the etiologic agent mycobacterium avium subsp. paratuberculosis (map) is necessary. the study was undertaken to assess the informative value of different typing techniques and individual genome markers by investigation of map transmission between wild-living red deer and farmed cattle with known shared habitats.53 map-type-ii-isolates were differentiated by short-sequence repeat (ssr, 4 loci)-, mycobact ...201122179249
tissue-specific expression of human calcineurin-binding protein 1 in mouse synovial tissue can suppress inflammatory arthritis.calcineurin (cn) is a calcium- and calmodulin-dependent serine/threonine phosphatase. in immune cells, cn controls the activity of a wide range of transcription factors, including nuclear factor of activated t, nuclear factor-kappa b, c-fos, and elk-1. cn plays an important role in synoviocyte activation and arthritis progression in vivo and this function is tightly linked to dysregulated intracellular ca(2+) store and ca(2+) response triggered by proinflammatory cytokines. in the present study, ...201122175542
first description of nodular onchocercosis (onchocerca jakutensis) in free-ranging italian red deer (cervus elaphus).onchocercosis is a vector-transmitted parasitic disease involving wild and domestic ungulates, humans, and dogs. red deer (cervus elaphus) host numerous onchocerca spp. which have precise anatomic sites in the host and two species, onchocerca flexuosa wedl, 1856 and onchocerca jakutensis guba-now, 1964, are found inside subcutaneous nodules. between september and november 2007, subcutaneous nodules were observed on both thighs in shot red deer of a tuscany population. we observed cystic structur ...201122102667
corrected misidentification of first instars of the bot flies cephenemyia apicata and c. jellisoni, with a review of the biology and life history of both species.first instars obtained from the uteri of host-seeking females, and compared with previously published descriptions of larvae collected from columbian black-tailed deer (odocoileus hemionus columbianus), revealed that larvae described as cephenemyia apicata bennett & sabrosky (diptera: oestridae) actually are c. jellisoni townsend. several new characters are added to supplement the original descriptions of the first instars of these species. collections of all larvae from deer autopsied during di ...201122168389
conservation of cervus elaphus meat in modified atmospheres.this study examines the effect of three types of modified atmospheres, each with a different gas composition (a:40% co(2)+60% n(2); b:80% co(2)+20% o(2); c:80% co(2)+20% n(2)), on the development of meat quality of cervus elaphus in order to suggest a gas composition that best preserves this type of meat. meat quality was assessed by examining ph, colour as l(*)a(*)b(*) values, drip loss (dl), cooking loss (cl) and shear force (sf). in samples of group a, ph values tended to be higher in all sto ...200322063440
dwarfism associated with chondrodysplasia in a red deer (cervus elaphus). 201122016513
variability of female responses to conspecific vs. heterospecific male mating calls in polygynous deer: an open door to hybridization?males of all polygynous deer species (cervinae) give conspicuous calls during the reproductive season. the extreme interspecific diversity that characterizes these vocalizations suggests that they play a strong role in species discrimination. however, interbreeding between several species of cervinae indicates permeable interspecific reproductive barriers. this study examines the contribution of vocal behavior to female species discrimination and mating preferences in two closely related polygyn ...201121887242
Morphogenetic aspects of deer antler development.Deer antlers are unique mammalian appendages that have potential for application as a valuable model for biomedical research, such as organ regeneration, bone development and growth control. In this review we described the morphogenetic aspects of antler development, by summarizing relevant information accumulated over the course of our antler research in combine with other published papers. Antler development consists of two distinct phenomena: generation and regeneration. Generation starts wit ...201222202000
molecular dynamics simulations and in silico peptide ligand screening of the elk-1 ets domain.abstract:201122044511
influence of packaging method and length of chilled storage on microflora, tenderness and colour stability of venison loins.sections of venison loins (ld) weighing approximately 300 g from 12 red deer (cervus elaphus) were packaged using four packaging methods: (a) vacuum packaging, (b) co(2) flushed using a nylon containment film (co(2)-nylon), (c) co(2) flushed using an ultra-high barrier containment film (co(2)-uhb), and (d) co(2) flushed using an aluminium foil laminate containment film (co(2)-foil) and stored for 1, 6, 12 and 18 weeks at 0°c. meat ph values were lower in all co(2) flushed meat packages (p<0·05) ...198822055411
activation of the intracellular metabotropic glutamate receptor 5 in striatal neurons leads to upregulation of genes associated with sustained synaptic transmission including arc/arg3.1.the g-protein coupled receptor, metabotropic glutamate receptor 5 (mglur5), is expressed on both cell surface and intracellular membranes in striatal neurons. using pharmacological tools to differentiate membrane responses, we previously demonstrated that cell surface mglur5 triggers rapid, transient cytoplasmic ca2+ rises resulting in c-jun n-terminal kinase, ca2+/calmodulin-dependent protein kinase (camk), and cyclic adenosine 3',5'-monophosphate-responsive element-binding protein (creb) phos ...201122179607
first molecular evidence of anaplasma ovis and rickettsia spp. in keds (diptera: hippoboscidae) of sheep and wild ruminants.to evaluate the presence of rickettsial agents in hippoboscid flies with molecular methods, 81 sheep keds (melophagus ovinus) were collected from 23 sheep, 144 deer keds (lipoptena cervi) were caught in the environment, and a further 463 and 59 individuals of the latter species were obtained from fresh carcasses of 29 red deer and 17 roe deer, respectively. dna was extracted individually or in pools. anaplasma ovis was demonstrated in all examined sheep keds, and from one pool of free-living dee ...201121923269
cholesterol concentration and fatty acid profile of red deer (cervus elaphus) meat.the effects of gender and age on intramuscular fat (imf) levels, cholesterol concentration, and fatty acid composition were investigated in the semitendinosus (st) and triceps brachii (tb) muscles of feral red deer (cervus elaphus). six stags of >2years of age, four hinds of 1year, and six calves of 6months were shot in slovenia. generally, all parameters measured were influenced by interaction of muscle and treatment group (hinds, stags and calves) at the 5% level or less. in st muscle, the imf ...200822063609
early growth response 1--a transcription factor in the crossfire of signal transduction cascades.early growth response-1 (egr-1) is a cys2-his2-type zinc-finger transcription factor. a broad range of extracellular stimuli is capable of activating egr-1, thus mediating growth, proliferation, differentiation or apoptosis. egr-1 is, therefore, participating in the progression of a variety of diseases such as atherosclerosis or cancer. functional response elements connect egr-1 to signal transduction cascades targeting egr-1. five serum response elements (sre) have been identified in the promot ...201122053691
campylobacter spp. distribution in biofilms on different surfaces in an agricultural watershed (elk creek, british columbia): using biofilms to monitor for campylobacter.despite its relevance to public health, presence and concentrations of campylobacter spp. in biofilms in natural aquatic environments has not been investigated. this study examined the occurrence of campylobacter spp. in biofilms on a variety of surfaces (river rock, slate rock, wood, lexan™, sandpaper, and sediment) and in water from december 2005 to december 2006 to find a substratum that facilitated campylobacters detection in natural aquatic environments. samples were collected at four sites ...201122204983
prostaglandin e(2) production in mice is reduced by consumption of range-fed sources of red meat.many view bison as a healthful alternative to other red meat sources, and as a way to decrease health risks, they associate it with meat consumption. using mice as a model for immune function, we hypothesized that consumption of meat from range-fed bison would decrease prostaglandin (pg) e(2) and alter prostacyclin (pgi(2)) release upon immune challenge when compared with mice fed meat from grain-finished bison, range-fed beef, feedlot steers, free-ranging elk, or chicken breast. after 2 weeks o ...201122153516
the inhibitory effects of nkx3.1 on igf-1r expression and its signalling pathway in human prostatic carcinoma pc3 cells.nkx3.1, which is a prostate-specific homeobox gene, plays an important role in prostate cancer and usually functions as a tumour suppressor gene. in this study, we investigated the inhibitory effect of nkx3.1 on insulin-like growth factor (igf)-1r expression and its downstream signalling pathway in pc3 cells. pc3 cells were stably transfected with nkx3.1 expression plasmid (pcdna3.1-nkx3.1) or vector plasmid (pcdna3.1+). the igf-ir mrna and protein expression levels were assessed in pc3-nkx3.1 t ...201122179513
Alpha and gamma herpesvirus detection in two herds of farmed red deer (Cervus elaphus) in New Zealand.Abstract201222175434
food chain model based on field data to predict westslope cutthroat trout (oncorhynchus clarkii lewisi) ovary selenium concentrations from water selenium concentrations in the elk valley, british columbia.previous studies conducted in the elk river watershed showed that selenium concentrations are higher in aquatic biota in lentic compared to lotic habitats of the system having similar water selenium concentrations. studies have also shown that water selenium concentrations have increased over time (∼10% per year) and recent annual average concentrations have ranged up to 0.044 mg/l in areas downstream from mine discharges. for the present study, trophic transfer of selenium was characterized in ...201122189838
bilateral microphthalmia and aphakia associated with multiple eye abnormalities in a free-living european red deer calf (cervus elaphus).a free-living european red deer calf (cervus elaphus) was euthanized due to bilateral microphthalmia. lens was missing, replaced by proliferating squamous epithelial cells; hyperplastic squamous cells, sebaceous and mucinous glands were observed within the cornea with the characteristics of inclusion cyst. findings were consistent with congenital microphthalmia/aphakia, with multiple eye abnormalities.201122134109
evidence for distinct chronic wasting disease (cwd) strains in experimental cwd in ferrets.chronic wasting disease (cwd) is an evolving prion disease of cervids (deer, elk and moose) that has been recognized in north america and korea. infection of non-cervid reservoir or transport species in nature is not reported. however, the ferret (mustela putorius furo) is susceptible to cwd after experimental inoculation. here, we report that infection of ferrets with either of two ferret cwd isolates by various routes of exposure has revealed biologically distinct strain-like properties distin ...201221918005
taenia arctos n. sp. (cestoda: cyclophyllidea: taeniidae) from its definitive (brown bear ursus arctos linnaeus) and intermediate (moose/elk alces spp.) hosts.taenia arctos n. sp. (cestoda: cyclophyllidea: taeniidae) is described from the brown bear ursus arctos linnaeus (definitive host) and moose/elk alces spp. (intermediate hosts) from finland (type-locality) and alaska, usa. the independent status of the new species and the conspecificity of its adults and metacestodes have been recently confirmed by the mtdna sequence data of lavikainen et al. (2011; parasitology international, 60, 289-295). special reference is given to morphological differences ...201122002024
dpp activates integrin-mediated anchorage-dependent signals in undifferentiated mesenchymal cells.dpp, a major noncollagenous protein of the dentin matrix is a highly acidic protein and binds ca2+ avidly and thus linked to matrix mineralization. here, we demonstrate that the rgd domain in dpp can bind to integrins on the cell surface of undifferentiated mesenchymal stem cells and pulp cells. this coupling generates intracellular signals that are channeled along cytoskeletal filaments and activates the non-receptor tyrosine kinase fak, which plays a key role in signaling at sites of cellular ...201122134916
historic, pre-european settlement, and present-day contribution of wild ruminants to enteric methane emissions in the united states.the objectives of this analysis were to estimated historic (pre-european settlement) enteric methane (ch(4)) emissions from wild ruminants in the contiguous united states and compare these to present-day ch(4) emissions from farmed ruminants. the analysis included bison, elk (wapiti), and deer (white-tailed and mule). wild ruminants such as moose, antelope (pronghorn), caribou, and mountain sheep and goat were not included in the analysis due to their natural range being mostly outside of the co ...201122178852
identification of meats from red deer (cervus elaphus), fallow deer (dama dama), and roe deer (capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12s rrna gene.polymerase chain reaction (pcr) based on oligonucleotide primers targeting the mitochondrial 12s rrna gene was applied to the specific identification of meats from red deer (cervus elaphus), fallow deer (dama dama), and roe deer (capreolus capreolus). the use of a common reverse primer, together with forward specific primers for red deer, fallow deer, and roe deer, allowed the selective amplification of the desired cervid sequences. the specificity of each primer pair was verified by pcr analysi ...200722064291
(-)-epigallocatechin gallate suppresses proliferation of vascular smooth muscle cells induced by high glucose by inhibition of pkc and erk1/2 signalings.proliferation of vascular smooth muscle cells (vsmcs) plays an important role in the development and progression of diabetes-related vascular complications. (-)-epigallocatechin gallate (egcg), the major catechin derived from green tea, is able to exert antidiabetes effects in animal models. however, it is not known whether or not egcg inhibits vsmc proliferation induced by high glucose. this study tested the hypothesis that egcg might have an inhibitory effect on vsmc proliferation induced by h ...201121973165
the influence of environment, mode of nutrition and animal species on level of nitrosamine contamination in venison.the work aimed to research occurrence of nitrosamines in raw venison from different game species and mode of nutrition effect on their meat concentrations. samples of venison from male and female elks, male and female red deer, male and female roe deer, male and female fallow deer, male and female wild boars, male and female brown hares and males and females of wild rabbits shot in poland during the permissible hunting seasons were used for the experiments after their jointing and cutting. in th ...200322063687
Inbreeding depression in red deer calves.ABSTRACT:201122039837
med23 mutation links intellectual disability to dysregulation of immediate early gene expression.med23 is a subunit of the mediator complex, a key regulator of protein-coding gene expression. here, we report a missense mutation (p. r617q) in med23 that cosegregates with nonsyndromic autosomal recessive intellectual disability. this mutation specifically impaired the response of jun and fos immediate early genes (iegs) to serum mitogens by altering the interaction between enhancer-bound transcription factors (tcf4 and elk1, respectively) and mediator. transcriptional dysregulation of these g ...201121868677
climate change intensification of herbivore impacts on tree recruitment.altered species interactions are difficult to predict and yet may drive the response of ecological communities to climate change. we show that declining snowpack strengthens the impacts of a generalist herbivore, elk (cervus elaphus), on a common tree species. thick snowpack substantially reduces elk visitation to sites; aspen (populus tremuloides) shoots in these areas experience lower browsing rates, higher survival and enhanced recruitment. aspen inside herbivore exclosures have greatly incre ...201121976686
a comparison of biochemical and meat quality variables in red deer (cervus elaphus) following either slaughter at pasture or killing at a deer slaughter plant.to investigate effects of pre-slaughter handling on blood and muscle biochemistry and venison quality, paddock-shot (n=8) and commercially slaughtered red deer (n=8) were compared. the deer were kept in two larger groups. one stag per group per day was head-shot, exsanguinated, electrically stimulated then transported 150 m to a deer slaughter premises (dsp) for processing. prior to each slaughter day one of the groups was mustered into a deer yards and six (including two experimental) deer were ...200222063109
sdf-1α induces pdgf-b expression and the differentiation of bone marrow cells into pericytes.platelet-derived growth factor b (pdgf-b) and its receptor, pdgfr-β, play a critical role in pericyte maturation; however, the mechanisms by which pdgf-b is upregulated in the tumor microenvironment remain unclear. we previously showed that upregulating stromal-derived factor, sdf-1α, in vegf(165)-inhibited ewing's sarcoma tumors (tc/sivegf(7-1)) induced pdgf-b mrna expression, increased infiltration and differentiation of bone marrow cells (bmc) into pericytes and, rescued tumor growth. the pur ...201121911740
mycobacterial infections in free-living cervids in germany (2002-2006).we examined 1,022 free-living roe deer, red deer, and fallow deer for mycobacteria in germany, 2002-2006. retropharyngeal lymph nodes and other tissues were processed for culture and isolates were identified with the use of polymerase chain reaction and dna sequencing. mycobacteria were found in 18.3% of deer, with mycobacterium avium in 14.8%. other atypical mycobacteria were detected in 5.3%. members of the mycobacterium tuberculosis complex were not detected.201122102674
Mercury exposure and mechanism of response in large game using the Almadén mercury mining area (Spain) as a case study.Mercury (Hg) accumulation, transfer, defense mechanism and adverse effects were studied in red deer (Cervus elaphus) and wild boar (Sus scrofa) from the Almadén mining district (Spain), the largest (285,000t of Hg) and the oldest (more than 2000 years) Hg mine/refining operation site in the world. Red deer (n=168) and wild boar (n=58) liver, kidney, bones (metacarpus), testis and muscle were analyzed for total Hg and selenium (Se) within a range of distances to the Almadén mining district. The h ...201122019248
electrical stimulation of red deer (cervus elaphus) carcasses - effects on rate of ph-decline, meat tenderness, colour stability and water-holding capacity.a total of 14 female red deer were included in a study on the effects of low voltage carcass electrical stimulation on meat tenderness, colour stability and water-holding capacity. carcasses were randomly allocated to either electrical stimulation treatment (es; 90-95 v unipolar pulses, 7.5 ms duration, 15 hz for a duration of 55 s) or no electrical stimulation (non-es) (n=7 in each group). temperature and ph decline was recorded in m.m. triceps brachii, longissimus dorsi et lumborum (at the las ...200122062680
authentication of meat from game and domestic species by snapshot minisequencing analysis.the aim of the present study is to develop an assay for the specific identification of meat from capreolus capreolus, cervus elaphus, capra ibex, rupicapra rupicapra, targeting sequences of the cytochrome b (cyt b) gene of mitochondrial dna. the assay is also intended to enable differentiation between meat from these wild species as well as ovis aries, capra hircus, bubalus bubalis, bos taurus and sus scrofa domestic species. the primers used in the preliminary pcr were designed in well conserve ...200822063325
unexpected high responses to tuberculin skin-test in farmed red deer: implications for tuberculosis control.tuberculosis (tb) in deer is a serious zoonotic disease of worldwide distribution. detection of infected animals is usually performed using single or comparative skin-testing (sst/cst), although false responses due to sensitization to other mycobacteria may occur, hampering diagnostic specificity. we describe the evolution of the responses to the sst, cst and to an in-house serological assay in a red deer farm subjected to regular tb testing in southern spain in an attempt to understand the dyna ...201122197268
influence of hunting-season stage and ripening conditions on nitrogen fractions and degradation of myofibrillar proteins in venison (cervus elaphus) chorizo sausages.the influence of hunting-season stage (deer hunted at the start versus deer hunted at the end of the season) and ripening conditions (natural versus controlled drying rooms) on nitrogen fractions and degradation of myofibrillar proteins were studied in chorizo sausages made with venison. variations of nitrogen fractions during 21 days of ripening were found. in all batches, myofibrillar protein content decreased and proteolysis indices were between 4.6% and 14.4% at the end of ripening. however, ...200722064193
Elk-1 Interacts with Dynein upon Serum Stimulation but Independent of Serine 383 Phosphorylation.Elk-1 belongs to the Ternary Complex Factor (TCF) subfamily of the ETS (from E26 viral oncogene) domain superfamily of transcription factors, and has been known as a regulator of mitogen-induced immediate early gene transcription upon Mitogen Activated Protein Kinase (MAPK) activation. Elk-1 has been previously shown to interact with neuronal microtubules, and here we show that P-S383-Elk-1, in addition to co-localizing with motor proteins kinesin, Eg5 and Mitotik Kinesin-Like Protein (MKLP) at ...201121935709
Novel regulatory mechanism and functional implication of plasminogen activator inhibitor-1 (PAI-1) expression in CpG-ODN-stimulated macrophages.Macrophages are activated by recognizing bacterial DNA and CpG-oligodeoxynucleotides (CpG-ODNs) through Toll-like receptor-9 (TLR-9). Plasminogen activator inhibitor-1 (PAI-1) has been shown to be an important factor in inflammation-induced macrophage migration which is essential for defense functions. The aim of this study was to demonstrate the molecular mechanism associated with the regulation of PAI-1 expression and its biological significance in CpG-ODN-stimulated mouse macrophages. Our res ...201222078208
Comparative analysis of essential collective dynamics and NMR-derived flexibility profiles in evolutionarily diverse prion proteins.Collective motions on ns-µs time scales are known to have a major impact on protein folding, stability, binding and enzymatic efficiency. It is also believed that these motions may have an important role in the early stages of prion protein misfolding and prion disease. In an effort to accurately characterize these motions and their potential influence on the misfolding and prion disease transmissibility we have conducted a combined analysis of molecular dynamic simulations and NMR-derived flexi ...201121869604
the ratio of growth differentiation factor 9: bone morphogenetic protein 15 mrna expression is tightly co-regulated and differs between species over a wide range of ovulation rates.recent evidence suggests that the species-specific ovulation-rate phenotypes may be influenced by differences in the expression levels of bone morphogenetic protein 15 (bmp15) and growth differentiation factor 9 (gdf9) mrna and protein. the aim of this study was to compare gdf9 and bmp15 mrna levels in individual denuded oocytes (do) from a range of single (i.e. cow, red deer), single-to-triple (i.e. sheep) and high (i.e. pig, mouse, rat) ovulation-rate species. compared to all other species stu ...201221970812
Relationships between in vivo microdamage and the remarkable regional material and strain heterogeneity of cortical bone of adult deer, elk, sheep and horse calcanei.Natural loading of the calcanei of deer, elk, sheep and horses produces marked regional differences in prevalent/predominant strain modes: compression in the dorsal cortex, shear in medial-lateral cortices, and tension/shear in the plantar cortex. This consistent non-uniform strain distribution is useful for investigating mechanisms that mediate the development of the remarkable regional material variations of these bones (e.g. collagen orientation, mineralization, remodeling rates and secondary ...201121951210
real-time pcr for detection and quantification of red deer (cervus elaphus), fallow deer (dama dama), and roe deer (capreolus capreolus) in meat mixtures.a rapid real-time polymerase chain reaction (pcr) technique using sybr green detection system, has been developed for the quantification of red deer, fallow deer, and roe deer dnas in meat mixtures. the method combines the use of cervid-specific primers that amplify a 134, 169, and 120bp of the 12s rrna gene fragment of red deer, fallow deer and roe deer, respectively, and universal primers that amplify a 140bp fragment on the nuclear 18s rrna gene from eukaryotic dna. the c(t) (threshold cycle) ...200822062757
selected beetle assemblages captured in pitfall traps baited with deer dung or meat in balsam fir and sugar maple forests of central quebec.vertebrate dung and carrion are rich and strongly attractive resources for numerous beetles that are often closely linked to them. the presence and abundance of beetles exploiting such resources are influenced by various ecological factors including climate and forest cover vegetation. we studied selected assemblages of coprophilous and necrophagous beetles in quebec along a 115-km north-south transect in three balsam fir (abies balsamea (l.) miller) forest sites and in a fourth forest site dom ...201022127165
Polymorphic integrations of an endogenous gammaretrovirus in the mule deer genome.Endogenous retroviruses constitute a significant genomic fraction in all mammalian species. Typically they are evolutionarily old and fixed in the host species population. Here we report on a novel endogenous gammaretrovirus (CrERV? for cervid endogenous gammaretrovirus) in the mule deer, Odocoileus hemionus that is insertionally polymorphic among individuals from the same geographical location, suggesting that it has a more recent evolutionary origin. Using PCR-based methods, we identified seve ...201122190723
risk factors associated with bruising in red deer at a commercial slaughter plant.bruising of slaughter animals has both economic and welfare implications. in order to identify potential risk factors we surveyed bruising of red deer carcasses from a single deer slaughter plant (dsp) over a three year period. downgrading due to bruising did not vary between the three years surveyed [1991, n = 21 454, 6.5% downgraded (dg); 1992, n = 22 289, 7.9% dg; 1993, n = 22 262, 6.1% dg]. from an intensive examination of one year's kill (1991) we related the incidence of bruising to variou ...199622060828
structure of the carboxy-terminal region of a kcnh channel.the kcnh family of ion channels, comprising ether-à-go-go (eag), eag-related gene (erg), and eag-like (elk) k(+)-channel subfamilies, is crucial for repolarization of the cardiac action potential, regulation of neuronal excitability and proliferation of tumour cells. the carboxy-terminal region of kcnh channels contains a cyclic-nucleotide-binding homology domain (cnbhd) and c-linker that couples the cnbhd to the pore. the c-linker/cnbhd is essential for proper function and trafficking of ion ch ...201222230959
vocal anatomy, tongue protrusion behaviour and the acoustics of rutting roars in free-ranging iberian red deer stags (cervus elaphus hispanicus).roaring in rutting iberian red deer stags cervus elaphus hispanicus is unusual compared to other subspecies of red deer, which radiated from the iberian refugium after the last glacial maximum. in all red deer stags, the larynx occupies a permanent low mid-neck resting position and is momentarily retracted almost down to the rostral end of the sternum during the production of rutting calls. simultaneous with the retraction of the larynx, male iberian red deer pronouncedly protrude the tongue dur ...201222257361
antioxidant activity of protein hydrolysates from aqueous extract of velvet antler (cervus elaphus) as influenced by molecular weight and enzymes.the crude protein hydrolysates from aqueous extract of velvet antler (aeva) were prepared by simulated gastrointestinal digestion (sgi, pepsin-pancreatin) using pancreatin-pepsin, alcalase and neutrase. the resulting hydrolysates were separated by sequential ultrafiltration into four fractions. the antioxidant activities of peptide fractions were evaluated by 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging, ferric reducing antioxidant power (frap), 2,2'-azinobis-(3-ethylbenzothiazoline-6 ...201122224289
"expression of protein kinase cαand the mzf-1 and elk-1 transcription factors in human breast cancer cells"."recently, our research into hepatocellular carcinoma (hcc) has shown that the transcription factors myeloid zinc finger-1 (mzf-1) and ets-like-protein 1 are related to protein kinase c alpha (pkcα) expression. the purpose of this study was to determine the correlation of the expression of pkcαwith the expressions of elk-1 and mzf-1 in various differentiated breast cancer cell lines: mda- mb-231, hs57bt, skbr3, mda-mb-468 and mcf-7. the malignant potential in the five lines of breast cancer cell ...201222242952
rapid detection of cwd prp: comparison of tests designed for the detection of bse or scrapie.chronic wasting disease (cwd) is a transmissible spongiform encephalopathy (tse) mainly affecting cervids in north america. the accumulation of an abnormal form of host-encoded prion protein (prp(cwd) ) in the cns and lymphoid tissues is characteristic of the disease and known to be caused by pathogenic prion proteins (prp(res) ), which are thought to be transmitted mainly by contact with body fluids, such like saliva. species known to be naturally infected by cwd include rocky mountain elk (cer ...201122212828
modelling transmission of bovine tuberculosis in red deer and wild boar in normandy, france.in early 2001, mycobacterium bovis infection was confirmed in red deer (rd) (cervus elaphus) shot in normandy region, france. an epidemiological survey conducted during the following hunting season in two connected forests confirmed the occurrence of the disease in both free-ranging rd and wild boar (wb) (sus scrofa). this was the first detected bovine tuberculosis outbreak in wildlife in france. we present a simple deterministic age-structured model of the within- and between-species m. bovis t ...201222958262
first detection of echinococcus granulosus g1 and g7 in wild boars (sus scrofa) and red deer (cervus elaphus) in romania using pcr and pcr-rflp techniques.the epidemiological status of ce in our country places romania into the top of the european countries and among the first countries worldwide. two hundred ninety wild animals (267 wild boars, 21 red deer and 2 mouflons) hunted in a private ground from bihor county from western romania were the subject of the necropsy in january 2012.out of 290 wild animals, 35 were positive during necropsy for hydatic cysts and from these, 33 wild boars and 2 red deer had hydatic cystsonly in the liver parenchym ...201223332123
giardia prevalence in wild cervids in poland.a total of 181 faecal samples were collected from wild cervids in two regions of poland. specimens were taken from 65 fallow deer (dama dama), 61 red deer (cervus elaphus), 50 roe deer (capreolus capreolus), and five moose (alces alces). giardia cysts were detected in one faecal specimen from the red deer and in two samples from the roe deer. although this study has demonstrated that giardia infection is remarkably rare in wild cervids, it should be emphasized that there are large populations of ...201223914615
host and pathogen dna identification in blood meals of nymphal ixodes ricinus ticks from forest parks and rural forests of poland.dna analysis of blood meals from unfed nymphal ixodes ricinus allows for the identification of tick host and tick-borne pathogens in the host species. the recognition of host species for tick larvae and the reservoirs of borrelia, rickettsia and anaplasma species were simultaneously carried out by analysis of the blood meals of 880 questing nymphal i. ricinus ticks collected in forest parks of szczecin city and rural forests in northwestern poland that are endemic areas for lyme borreliosis. the ...201324352572
attachment site selection of life stages of ixodes ricinus ticks on a main large host in europe, the red deer (cervus elaphus).ticks and tick-borne diseases are increasing in many areas of europe and north america due to climate change, while land use and the increased abundances of large hosts play a more controversial role. the pattern of host selection involves a crucial component for tick abundance. while the larvae and nymphs feed on a wide range of different sized hosts, the adult female ticks require blood meal from a large host (>1 kg), typically a deer, to fulfil the life cycle. understanding the role of differ ...201425391754
babesia spp. in european wild ruminant species: parasite diversity and risk factors for infection.babesia are tick-borne parasites that are increasingly considered as a threat to animal and public health. we aimed to assess the role of european free-ranging wild ruminants as maintenance mammalian hosts for babesia species and to determine risk factors for infection. edta blood was collected from 222 roe deer (capreolus c. capreolus), 231 red deer (cervus e. elaphus), 267 alpine chamois (rupicapra r. rupicapra) and 264 alpine ibex (capra i. ibex) from all over switzerland and analysed by pcr ...201424925474
vertical transmission of bartonella schoenbuchensis in lipoptena cervi.lipoptena cervi (diptera: hippoboscidae) is a hematophagous ectoparasite of cervids, which is considered to transmit pathogens between animals and occasionally to humans. the principal life stage that is able to parasitize new hosts is a winged ked that just emerged from a pupa. to facilitate efficient transmission of pathogens between hosts, vertical transmission from female deer keds to their offspring is necessary. we investigated vertical transmission of several vector-borne pathogens associ ...201525889985
the influence of red deer space use on the distribution of ixodes ricinus ticks in the landscape.many wingless ectoparasites have a limited capacity for active movement and are therefore primarily dependent on hitchhiking on their hosts for transportation. the distribution of the tick ixodes ricinus is expected to depend mainly on transportation by hosts and tick subsequent survival in areas where they drop off. in europe, the most important hosts of adult female i. ricinus are cervids. the extensive space use of large hosts provides a much larger dispersal potential for i. ricinus than tha ...201627737695
molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from portugal.wildlife can act as reservoir of different tick-borne pathogens, such as bacteria, parasites and viruses. the aim of the present study was to assess the presence of tick-borne bacteria and protozoa with veterinary and zoonotic importance in cervids and wild boars from the centre and south of portugal.201627160767
isolation of digital dermatitis treponemes from hoof lesions in wild north american elk (cervus elaphus) in washington state, usa.since 2008, a large increase in the numbers of cases of lameness have been seen in wild north american elk (cervus elaphus) from washington state, usa. the most recent cases manifested as foot lesions similar both clinically and pathologically to those seen in digital dermatitis (dd) in cattle and sheep, a disease with a bacterial etiopathogenesis. to determine whether the same bacteria considered responsible for dd are associated with elk lameness, lesion samples were subjected to bacterial iso ...201425355757
pathogens at the livestock-wildlife interface in western alberta: does transmission route matter?in southwestern alberta, interactions between beef cattle and free-ranging elk (cervus elaphus) may provide opportunities for pathogen transmission. to assess the importance of the transmission route on the potential for interspecies transmission, we conducted a cross-sectional study on four endemic livestock pathogens with three different transmission routes: bovine viral diarrhea virus and bovine herpesvirus 1 (predominantly direct transmission), mycobacterium avium subsp. paratuberculosis (ma ...201424517283
spatio-temporal trends and risk factors affecting west nile virus and related flavivirus exposure in spanish wild ruminants.during the last decade, the spread of many flaviviruses (genus flavivirus) has been reported, representing an emerging threat for both animal and human health. to further study utility of wild ruminant samples in west nile virus (wnv) surveillance, we assessed spatio-temporal trends and factors associated with wnv and cross-reacting flaviviruses exposure, particularly usutu virus (usuv) and meaban virus (mbv), in wild ruminants in spain. serum samples from 4693 wild ruminants, including 3073 fre ...201627829427
foraging ecology of three sympatric ungulate species - behavioural and resource maps indicate differences between chamois, ibex and red deer.the spatial distribution of forage resources is a major driver of animal movement patterns. understanding where animals forage is important for the conservation of multi-species communities, since interspecific competition can emerge if different species use the same depletable resources. however, determining forage resources in a spatially continuous fashion in alpine grasslands at high spatial resolution was challenging up to now, because terrain heterogeneity causes vegetation characteristics ...201526807258
molecular identification of theileria parasites of northwestern chinese cervidae.theileria and babesia protozoan parasites are transmitted mainly by tick vectors. these parasites cause heavy economic losses to the live-stock industry, as well as affecting the health of wild animals in parasite-endemic areas. identification of infectious agents in wild animals is not only crucial for species preservation, but also provides valuable information on parasite epidemiology. here, we conducted a molecular surveillance study in northwestern china to assess the prevalence of blood pa ...201424885179
climate and environmental change drives ixodes ricinus geographical expansion at the northern range margin.global environmental change is causing spatial and temporal shifts in the distribution of species and the associated diseases of humans, domesticated animals and wildlife. in the on-going debate on the influence of climate change on vectors and vector-borne diseases, there is a lack of a comprehensive interdisciplinary multi-factorial approach utilizing high quality spatial and temporal data.201424401487
prevalence of neoehrlichia mikurensis in ticks and rodents from north-west europe.neoehrlichia mikurensis s an emerging and vector-borne zoonosis: the first human disease cases were reported in 2010. limited information is available about the prevalence and distribution of neoehrlichia mikurensis in europe, its natural life cycle and reservoir hosts. an ehrlichia-like schotti variant has been described in questing ixodes ricinus ticks, which could be identical to neoehrlichia mikurensis.201222515314
anaplasma phagocytophilum strains from voles and shrews exhibit specific anka gene sequences.anaplasma phagocytophilum is a gram-negative bacterium that replicates obligate intracellularly in neutrophils. it is transmitted by ixodes spp. ticks and causes acute febrile disease in humans, dogs, horses, cats, and livestock. because a. phagocytophilum is not transmitted transovarially in ixodes spp., it is thought to depend on reservoir hosts to complete its life cycle. in europe, a. phagocytophilum was detected in roe deer, red deer, wild boars, and small mammals. in contrast to roe deer, ...201324283328
landscape level variation in tick abundance relative to seasonal migration in red deer.partial migration is common among northern ungulates, typically involving an altitudinal movement for seasonally migratory individuals. the main driving force behind migration is the benefit of an extended period of access to newly emerged, high quality forage along the green up gradient with increasing altitude; termed the forage maturation hypothesis. any other limiting factor spatially correlated with this gradient may provide extra benefits or costs to migration, without necessarily being th ...201323951125
spatial and seasonal variation in the prevalence of anaplasma phagocytophilum and borrelia burgdorferi sensu lato in questing ixodes ricinus ticks in norway.understanding the variation in prevalence of borrelia burgdorferi sensu lato (lyme borreliosis spirochaetes, lbs) and anaplasma phagocytophilum (causing tick-borne fever in ruminants and human granulocytic ehrlichiosis) in ticks is vital from both a human and an animal disease perspective to target the most effective mitigation measures. from the host competence hypothesis, we predicted that prevalence of lbs would decrease with red deer density, while prevalence of a. phagocytophilum would incr ...201323786850
correlation of tbe incidence with red deer and roe deer abundance in slovenia.tick-borne encephalitis (tbe) is a virus infection which sometimes causes human disease. the tbe virus is found in ticks and certain vertebrate tick hosts in restricted endemic localities termed tbe foci. the formation of natural foci is a combination of several factors: the vectors, a suitable and numerous enough number of hosts and in a habitat with suitable vegetation and climate. the present study investigated the influence of deer on the incidence of tick-borne encephalitis. we were able to ...201323776668
anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in south-western slovakia.ixodes ricinus is the principal vector of anaplasma phagocytophilum, the ethiological agent of granulocytic anaplasmosis in europe. anaplasmosis is an emerging zoonotic disease with a natural enzootic cycle. the reservoir competence of rodents is unclear. monitoring of a. phagocytophilum prevalence in i. ricinus and rodents in various habitat types of slovakia may contribute to the knowledge about the epidemiology of anaplasmosis in central europe.201525980768
a new multiple-locus variable-number tandem repeat analysis reveals different clusters for anaplasma phagocytophilum circulating in domestic and wild ruminants.anaplasma phagocytophilum is a tick-borne intragranulocytic alpha-proteobacterium. it is the causative agent of tick-borne fever in ruminants, and of human granulocytic anaplasmosis in humans, two diseases which are becoming increasingly recognized in europe and the usa. however, while several molecular typing tools have been developed over the last years, few of them are appropriate for in-depth exploration of the epidemiological cycle of this bacterium. therefore we have developed a multiple-l ...201425228371
prevalence and diversity among anaplasma phagocytophilum strains originating from ixodes ricinus ticks from northwest norway.the tick-borne pathogen anaplasma phagocytophilum causes great concern for livestock farmers. tick-borne fever is a widespread disease in norway, and antibodies have been produced amongst sheep, roe deer, red deer, and moose. the main vector ixodes ricinus is found along the norwegian coastline as far north as the arctic circle. a total number of 1804 i. ricinus ticks were collected and the prevalence of the pathogen was determined by species-specific qpcr. the overall infection rate varied from ...201425215241
piroplasmosis in wildlife: babesia and theileria affecting free-ranging ungulates and carnivores in the italian alps.piroplasmosis are among the most relevant diseases of domestic animals. babesia is emerging as cause of tick-borne zoonosis worldwide and free-living animals are reservoir hosts of several zoonotic babesia species. we investigated the epidemiology of babesia spp. and theileria spp. in wild ungulates and carnivores from northern italy to determine which of these apicomplexan species circulate in wildlife and their prevalence of infection.201424533742
isolation and characterization of babesia pecorum sp. nov. from farmed red deer (cervus elaphus).the diversity of babesia species infecting cervids in parts of central and southern spain was analyzed by collecting blood from farmed red deer (cervus elaphus). babesia sp. was isolated in vitro from two red deer herds in cádiz and ciudad real. the number of babesia sp. carriers differed between the two herds: 36/77 in cádiz and 1/35 in ciudad real. hyalomma lusitanicum was the most prevalent tick species identified on the cádiz farm vegetation and on sampled animals, and is therefore a candida ...201425155988
sex-biased differences in the effects of host individual, host population and environmental traits driving tick parasitism in red deer.the interactions between host individual, host population, and environmental factors modulate parasite abundance in a given host population. since adult exophilic ticks are highly aggregated in red deer (cervus elaphus) and this ungulate exhibits significant sexual size dimorphism, life history traits and segregation, we hypothesized that tick parasitism on males and hinds would be differentially influenced by each of these factors. to test the hypothesis, ticks from 306 red deer-182 males and 1 ...201323819112
seroprevalence and risk factors associated to mycobacterium bovis in wild artiodactyl species from southern spain, 2006-2010.the control of bovine tuberculosis (btb) is at a critical point in the last stage of eradication in livestock. wildlife species recently have emerged infected with tb in europe, particularly ungulates in the iberian peninsula. epidemiological information regarding tb in wild ungulates including affected species, prevalence, associated risk factors and appropriate diagnostic methods need to be obtained in these countries. a cross-sectional study was carried out on wild artiodactyl species, includ ...201222523563
the epidemiology of mycobacterium bovis in wild deer and feral pigs and their roles in the establishment and spread of bovine tuberculosis in new zealand wildlife.in new zealand, wild deer and feral pigs are assumed to be spillover hosts for mycobacterium bovis, and so are not targeted in efforts aimed at locally eradicating bovine tuberculosis (tb) from possums (trichosurus vulpecula), the main wildlife host. here we review the epidemiology of tb in deer and pigs, and assess whether new zealand's tb management programme could be undermined if these species sometimes achieve maintenance host status. in new zealand, tb prevalences of up to 47% have been re ...201525295713
country, cover or protection: what shapes the distribution of red deer and roe deer in the bohemian forest ecosystem?the bohemian forest ecosystem encompasses various wildlife management systems. two large, contiguous national parks (one in germany and one in the czech republic) form the centre of the area, are surrounded by private hunting grounds, and hunting regulations in each country differ. here we aimed at unravelling the influence of management-related and environmental factors on the distribution of red deer (cervus elaphus) and roe deer (capreolus capreolus) in this ecosystem. we used the standing cr ...201525781942
diet of wolves canis lupus returning to hungary.at the end of the nineteenth century, the wolf canis lupus was extinct in hungary and in recent decades has returned to the northern highland area of the country. the diet of wolves living in groups in aggteleki national park was investigated using scat analysis (n = 81 scats) and prey remains (n = 31 carcasses). throughout the year wolves (average, minimum two wolves per year) consumed mostly wild-living ungulates (mean percent of biomass consumed, b% 97.2%; relative frequency of occurrence, %o ...201122448046
a two-years' survey on the prevalence of tuberculosis caused by mycobacterium caprae in red deer (cervus elaphus) in the tyrol, austria.a survey of 143 hunter-harvested red deer for tuberculosis was conducted in an alpine area in western austria over two subsequent years. there, single tuberculosis cases caused by mycobacterium caprae had been detected in cattle and red deer over the preceding decade. the area under investigation covered approximately 500 km(2), divided into five different hunting plots. lymph nodes of red deer were examined grossly and microscopically for typical tuberculosis-like lesions and additionally by mi ...201223762580
a bayesian approach to study the risk variables for tuberculosis occurrence in domestic and wild ungulates in south central spain.bovine tuberculosis (btb) is a chronic infectious disease mainly caused by mycobacterium bovis. although eradication is a priority for the european authorities, btb remains active or even increasing in many countries, causing significant economic losses. the integral consideration of epidemiological factors is crucial to more cost-effectively allocate control measures. the aim of this study was to identify the nature and extent of the association between tb distribution and a list of potential r ...201222931852
toxoplasma gondii in sympatric wild herbivores and carnivores: epidemiology of infection in the western alps.toxoplasma gondii is an apicomplexan parasite that is able to infect almost all warm blooded animals. in europe, the domestic cat is the main definitive host. worldwide, 6 billion people are infected with this parasite. the goal of our research is to evaluate the prevalence of t. gondii infection in wild animals from a previously unsampled area in northern italy where 0.1% of women seroconvert during pregnancy each year.201424766665
reliable discrimination of 10 ungulate species using high resolution melting analysis of faecal dna.identifying species occupying an area is essential for many ecological and conservation studies. faecal dna is a potentially powerful method for identifying cryptic mammalian species. in new zealand, 10 species of ungulate (order: artiodactyla) have established wild populations and are managed as pests because of their impacts on native ecosystems. however, identifying the ungulate species present within a management area based on pellet morphology is unreliable. we present a method that enables ...201424637802
getting the biggest birch for the bang: restoring and expanding upland birchwoods in the scottish highlands by managing red deer.high deer populations threaten the conservation value of woodlands and grasslands, but predicting the success of deer culling, in terms of allowing vegetation to recover, is difficult. numerical simulation modeling is one approach to gain insight into the outcomes of management scenarios. we develop a spatially explicit model to predict the responses of betula spp. to red deer (cervus elaphus) and land management in the scottish highlands. our model integrates a bayesian stochastic stage-based m ...201323919137
multilevel d-loop pcr identification of hunting game.the control region of mtdna (d-loop) was used for hair samples of the five hunting game species identification: red deer (cervus elaphus), roe deer (capreolus capreolus), fallow deer (dama dama), mouflon (ovis aries musimon), and wild boar (sus scrofa). for d-loop multilevel pcr detection scheme was applied in six primers (ce cvzv 1 = 5'-gatcacgagcttgatcacca-3'; ce cvzv 2 = 5'-aggagtgggcgattttaggt-3'; dd cvzv 3 = 5'-cgcgtgaaaccaacaacccgc-3'; dd cvzv 4 = 5'-ccgggtcggggccttagacg-3'; ssw cvzv 5 = 5 ...201327275406
toward an identification of resources influencing habitat use in a multi-specific context.interactions between animal behaviour and the environment are both shaping observed habitat use. despite the importance of inter-specific interactions on the habitat use performed by individuals, most previous analyses have focused on case studies of single species. by focusing on two sympatric populations of large herbivores with contrasting body size, we went one step beyond by studying variation in home range size and identifying the factors involved in such variation, to define how habitat f ...201122216164
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