| in-vitro acquisition of iron from the iron---desferrioxamine complex by aeromonas hydrophila. | | 1996 | 11866778 |
| immuno-capture pcr for detection of aeromonas hydrophila. | in this report, we describe the use of universal primer pcr (uppcr) for the detection of 16s ribosomal rna (rrna) genes from aeromonas hydrophila captured by anti-a. hydrophila antibody coupled to a microplate. the approach combining immuno-capture with uppcr provides a quick, sensitive, and reproducible way for the detection of bacterial cells. | 2002 | 11869801 |
| molecular and culture-based analyses of prokaryotic communities from an agricultural soil and the burrows and casts of the earthworm lumbricus rubellus. | the microbial populations in no-till agricultural soil and casts of the earthworm lumbricus rubellus were examined by culturing and molecular methods. clone libraries of the 16s rrna genes were prepared from dna isolated directly from the soil and earthworm casts. although no single phylum dominated the soil library of 95 clones, the largest numbers of clones were from acidobacteria (14%), cytophagales (13%), chloroflexi (8%), and gamma-proteobacteria (8%). while the cast clone library of 102 cl ... | 2002 | 11872477 |
| microbial populations of an upflow anaerobic sludge blanket reactor treating wastewater from a gelatin industry. | the microbial populations of an upflow anaerobic sludge blanket reactor, used for treating wastewater from the gelatin industry, were studied by microbiological methods and phase-contrast and electron microscopy. microscopy examination of the sludge showed a complex mixture of various rod-shaped and coccoid bacterial pluslong filaments and verymobile curved rods. in addition free-living anaerobic ciliates and flagellates were also observed. the trophic group population observed in decreasing ord ... | 2001 | 11873883 |
| [diagnosis of pnh by detecting the resistance of rbcs to the hemolytic effect of hec toxin secreted by aeromonus hydrophila]. | to study the feasibility of diagnosing paroxysmal nocturnal hemoglobinuria (pnh) with hec toxin (secreted by aeromonus hydrophila) test. | 2000 | 11877028 |
| surface characters and extracellular toxins involved in the pathogenesis of aeromonas hydrophila. | a small number of serotypically distinct strains of a. hydrophila obtained from diseased freshwater fish were examined for their pathogenic properties comprising of cell surface characteristics and extracellular toxins. test strains exhibited homogeneity in their cell surface characteristics despite being serologically heterogeneous. studies on extracellular biological activities revealed qualitative and quantitative differences in production of toxins, probably explaining their antigenic divers ... | 2001 | 11883514 |
| hydrolysis of thionopeptides by the aminopeptidase from aeromonas proteolytica: insight into substrate binding. | a series of l-leucine aniline analogues were synthesized that contained either a carbonyl or thiocarbonyl as a part of the amide bond. additionally, the para-position on the phenyl ring of several substrates was altered with various electron-withdrawing or donating groups. the kinetic constants k(m) and k(cat) were determined for the hydrolysis of each of these compounds in the presence of the aminopeptidase from aeromonas proteolytica (aap) containing either zn(ii) or cd(ii). the dizinc(ii) for ... | 2002 | 11888288 |
| characterization of an adp-ribosyltransferase toxin (aext) from aeromonas salmonicida subsp. salmonicida. | an adp-ribosylating toxin named aeromonas salmonicida exoenzyme t (aext) in a. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. gene aext, encoding toxin aext, was cloned and characterized by sequence analysis. aext shows significant sequence similarity to the exos and exot exotoxins of pseudomonas aeruginosa and to the yope cytotoxin of different yersinia species. the aext gene was detected in all of the 12 a. salmonicida subsp. salmonicida strai ... | 2002 | 11889090 |
| [a case of severe septicemia due to aeromonas hydrophila]. | septicemia by aeromonas hydrophila (a. hydrophila) developed in a 69-year-old female with a history of uncompensated liver cirrhosis. she was admitted to our hospital, complaining of fever, vomiting, diarrhea, and pain as well as swelling of the left lower extremity. seven hours later, skin of the left extremity developed bullae and became discolored. although she was treated with infusion of antibiotics plus dopamine, continuous hemodiafiltration, glucose-insulin therapy and mechanical ventilat ... | 2002 | 11889792 |
| aeromonas jandaei cellulitis and bacteremia in a man with diabetes. | | 2002 | 11893376 |
| role of various enterotoxins in aeromonas hydrophila-induced gastroenteritis: generation of enterotoxin gene-deficient mutants and evaluation of their enterotoxic activity. | three enterotoxins from the aeromonas hydrophila diarrheal isolate ssu have been molecularly characterized in our laboratory. one of these enterotoxins is cytotoxic in nature, whereas the other two are cytotonic enterotoxins, one of them heat labile and the other heat stable. earlier, by developing an isogenic mutant, we demonstrated the role of a cytotoxic enterotoxin in causing systemic infection in mice. in the present study, we evaluated the role of these three enterotoxins in evoking diarrh ... | 2002 | 11895956 |
| differences in the microflora of scarified and unscarified seeds of karwinskia humboldtiana (rhamnaceae). | seeds of karwinskia humboldtiana obtained from a 1997 collection in the locality of villa de gracía nuevo (león, mexico) were contaminated with spores of filamentous fungi, bacteria and yeasts. the concentration of microorganisms in unscarified seeds ranged from 3.0 x 10(3) to 7.5 x 10(3) cfu/g. predominant were bacterial isolates of the genera aeromonas sp., bacillus, and pseudomonas; from filamentous fungi were identified alternaria, aspergillus niger, cladosporium sp., fusarium sp., mucor sp. ... | 2001 | 11898346 |
| effects of refrigeration and alcohol on the load of aeromonas hydrophila in oysters. | members of the bacterial genus aeromonas are widely distributed throughout the environment and are readily cultured from a variety of foods. one member of this genus, aeromonas hydrophila, has been reputed to be a significant cause of gastrointestinal disease. in this study, we examined the effects of refrigeration and alcohol on the level of a. hydrophila in oysters. specifically, vodka was examined because it is used by the food service industry in preparation of oysters romanoff. one set of o ... | 2002 | 11899058 |
| aeromonas species in foods. | aeromonas species have been recognized as potential or emerging foodborne pathogens for more than 20 years. aeromonads are estuarine bacteria and are ubiquitous in fresh water, fish and shellfish, meats, and fresh vegetables. actual sourced foodborne outbreaks are few, but epidemiological evidence suggests that the bacterium can cause self-limiting diarrhea, with children being the most susceptible population. most aeromonads are psychrotrophic and can grow in foods during cold storage. aeromona ... | 2002 | 11899061 |
| in vitro antimicrobial susceptibility of clinical isolates of aeromonas caviae, aeromonas hydrophila and aeromonas veronii biotype sobria. | | 2002 | 11909850 |
| recent developments in carbapenems. | carbapenems are beta-lactam antibiotics characterised by the presence of a beta-lactam ring with a carbon instead of sulfone in the 4-position of the thyazolidinic moiety. the first carbapenem to be utilised in therapy was imipenem, the n-formimidoyl derivative of thienamycin. imipenem is coadministered with cilastatin, an inhibitor of human renal dehydropeptidase i, as imipenem is hydrolysed by this enzyme. meropenem was the first carbapenem with a 1-beta-methyl group and 2-thio pyrrolidinyl mo ... | 2002 | 11922861 |
| identification of mouse submaxillary gland protein in mouse saliva and its binding to mouse oral bacteria. | the mouse submaxillary gland protein (msmgp) is highly expressed in the submandibular gland of the adult mouse and rat. it shares 51% identity at the amino-acid level with a human protein, the prolactin-inducible protein (pip)/gross cystic disease fluid protein 15 (gcdfp-15), which has been found in saliva, tears, sweat, seminal plasma, submucosal glands of the lung and amniotic fluid. more recently, the human pip has been reported to bind to bacterial strains normally found in the mouth, ear ca ... | 2002 | 11922875 |
| change in the composition of the microflora on vacuum-packaged beef during chiller storage. | five fresh beefcut samples were divided into 10 pieces, respectively, vacuum-packaged and stored at 2 degrees c for up to 6 weeks. the average ph values of five pieces from five beef samples were 5.62 +/- 0.04 at the start of storage and 5.12 +/- 0.07 after 6 weeks of storage. the pieces were homogenized, diluted and cultivated at weekly intervals on glucose-blood-liver (bl), de man rogosa and sharpe (mrs) and trypticase soya (ts) agars at 7 degrees c for 10-14 days. from plates with 30-300 colo ... | 2002 | 11929174 |
| production of superoxide anion by head-kidney leucocytes of indian major carps immunised with bacterins of aeromonas hydrophila. | on contact with micro-organisms or any other antigenic particles which are recognised as phagocytosable, the leucocytes of vertebrates raise their oxygen consumption suddenly (respiratory burst). the consumed oxygen is then converted into reactive oxygen species (ros). an effort has been made in the present study to estimate the production of superoxide anion, one of the ros, by the resident as well as activated head-kidney leucocytes of indian major carps. activation was accomplished by immunis ... | 2002 | 11931016 |
| comparsion of selected growth media for culturing serratia marcescens, aeromonas sp., and pseudomonas aeruginosa as pathogens of adult stomoxys calcitrans (diptera: muscidae). | stable flies, stomoxys calcitrans (l.), were orally infected with aeromonas sp., pseudomonas aeruginosa (schroeter), and serratia marcescens bizio that were cultured on egg-yolk media, nutrient broth, and fly egg media. aeromonas and serratia caused mortality when the bacteria were originally grown on egg-yolk medium. pseudomonas was equally lethal regardless of the media on which it was cultured. a wild isolate of aeromonas caused greater death than an isolate that had been passed through host ... | 2002 | 11931277 |
| predictive modelling for packaging design: equilibrium modified atmosphere packages of fresh-cut vegetables subjected to a simulated distribution chain. | the impact of temperature fluctuations in a simulated cold distribution chain, typical of commercial practice, was investigated on both the microbial and sensorial quality of equilibrium modified atmosphere (ema) packaged minimally processed vegetables. the internal o2 concentration of the designed packages could be predicted for the different steps of the simulated distribution chain by applying an integrated mathematical system. the internal atmosphere in the packages remained in its aerobic r ... | 2002 | 11934040 |
| incidence of toxic aeromonas isolated from food and human infection. | one hundred and ninety four aeromonas isolates (99 from food and 95 from clinical sources) were analyzed as to the species involved and the toxins produced. of the clinical isolates of aeromonas, 29.4% were enterotoxigenic, 43.1% were hemolytic and 89% were cytotoxigenic. among the food isolates, 18.2% were enterotoxigenic, 17.1% were hemolytic and 72.7% were cytotoxigenic. aeromonas sobria and aeromonas veronii produced more enterotoxin and cytotoxin than the other isolates, whereas a. veronii ... | 2002 | 11934569 |
| potential virulence and antimicrobial susceptibility of aeromonas popoffii recovered from freshwater and seawater. | aeromonas popoffii is the most recent species within the genus aeromonas described from freshwater. in our study this species was also recovered from this habitat and for the first time from seawater. most of the virulence factors known in aeromonas spp. (aerolysin/hemolysin, serine protease, lipases and dnases) were highly prevalent in this species. third-generation cephalosporins and quinolones were the most active antimicrobial agents against a. popoffii. | 2002 | 11934570 |
| double-edged role of statins in angiogenesis signaling. | 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitors (statins) exert potent vasculoprotective effects. however, the potential contribution to angiogenesis is controversial. in the present study, we demonstrate that atorvastatin dose-dependently affects endothelial cell migration and angiogenesis. in vivo relevant concentrations of 0.01 to 0.1 micromol/l atorvastatin or mevastatin promote the migration of mature endothelial cells and tube formation. moreover, atorvastatin also increases mig ... | 2002 | 11934843 |
| production of n-acetyl-d-glucosamine from alpha-chitin by crude enzymes from aeromonas hydrophila h-2330. | the selective and efficient production of n-acetyl-d-glucosamine (glcnac) was achieved from flake type of alpha-chitin by using crude enzymes derived from aeromonas hydrophila h-2330. | 2002 | 11950472 |
| novel aeromonas hydrophila ppd134/91 genes involved in o-antigen and capsule biosynthesis. | the sequences of the o-antigen and capsule gene clusters of the virulent aeromonas hydrophila strain ppd134/91 were determined. the o-antigen gene cluster is 17,296 bp long and comprises 17 genes. seven pathway genes for the synthesis of rhamnose and mannose, six transferase genes, one o unit flippase gene, and one o-antigen chain length determinant gene were identified by amino acid sequence similarity. pcr and southern blot analysis were performed to survey the distribution of these 17 genes a ... | 2002 | 11953367 |
| aeromonas hydrophila vacuolating activity in the caco-2 human enterocyte cell line as a putative virulence factor. | a non-enterotoxigenic strain of aeromonas hydrophila isolated from diarrheic stools of an 8-month-old child was found to cause vacuolation in caco-2 cells. the vacuoles became prominent 60 min after addition of the bacterial culture to the cell monolayers and, after 120 min, a complete disruption of the monolayers was observed. cell vacuolation was not detected when caco-2 monolayers were tested with sterile filtrates of overnight cultures of the a. hydrophila vacuolating strain ah14846. this st ... | 2002 | 11958929 |
| [effect of bacterial infection on the lipid composition of carp blood lymphocytes]. | the initial stage of bacterial infection is characterized by an increase in the level of total lipids and polyenoic fatty acids in membrane phospholipids of blood lymphocytes of carp (cyprinus carpio l.). in the fish infected with aeromonads, changes in the ratios of fatty acids in phospholipids are similar for either lymphocytes, liver, or whole blood. the extent to which these changes are pronounced depends on the original physiological status of the fish. | 2002 | 11962218 |
| the regulation of biofilm development by quorum sensing in aeromonas hydrophila. | aeromonas hydrophila is an opportunistic gram-negative pathogen that readily attaches to stainless steel to produce a thin biofilm with a complex 3d structure covering 40-50% of the available surface and producing large microcolonies. as a. hydrophila possesses an n-acylhomoserine lactone (ahl)-dependent quorum-sensing system based on the ahyri locus, the presence of the ahyi protein and c4-hsl within the biofilm phase was first established by western blot and ahl biosensor analysis respectively ... | 2002 | 11966822 |
| expression of the exeab complex of aeromonas hydrophila is required for the localization and assembly of the exed secretion port multimer. | aeromonas hydrophila secretes protein toxins via the type ii pathway, involving the products of at least two operons, exeab (gspab) and exec-n (gspc-n). in the studies reported here, aerolysin secretion was restored to c5.84, an exea::tn5-751 mutant, by overexpression of exed alone in trans. expression studies indicated that these results did not reflect a role of exeab in the regulation of the exec-n operon. instead, immunoblot analysis showed that exed did not multimerize in c5.84, and fractio ... | 2002 | 11967081 |
| substrate-activated zinc binding of metallo-beta -lactamases: physiological importance of mononuclear enzymes. | we have investigated the influence of substrate binding on the zinc ion affinity of representatives from the three metallo-beta-lactamase subclasses, b1 (bcii from bacillus cereus and blab from chryseobacterium meningosepticum), b2 (cpha from aeromonas hydrophila), and b3 (l1 from stenotrophomonas maltophilia). by competition experiments with metal-free apoenzymes and chromophoric zinc chelators or edta, we determined the dissociation constants in the absence and presence of substrates. for the ... | 2002 | 11967267 |
| abdominal infections in patients with acute leukaemia: a prospective study applying ultrasonography and microbiology. | a prospective study of 62 chemotherapy-induced neutropenic episodes in patients with acute leukaemia was conducted to determine the incidence and causes of abdominal infections, and to assess the diagnostic value of the combined use of ultrasonography (us) and microbiology. each patient underwent us of liver, gallbladder and complete bowel before chemotherapy, on days 2-4 after the end of chemotherapy and in cases of fever, diarrhoea or abdominal pain. us was combined with a standardized clinica ... | 2002 | 11972517 |
| production of a monoclonal antibody against aeromonas hydrophila and its application to bacterial identification. | to develop a monoclonal antibody (mab) for the rapid detection of aeromonas hydrophila in human faeces. | 2002 | 11972699 |
| enhanced accumulation and changed monomer composition in polyhydroxyalkanoate (pha) copolyester by in vitro evolution of aeromonas caviae pha synthase. | by in vitro evolution experiment, we have first succeeded in acquiring higher active mutants of a synthase that is a key enzyme essential for bacterial synthesis of biodegradable polyester, polyhydroxyalkanoate (pha). aeromonas caviae fa440 synthase, termed phac(ac), was chosen as a good target for evolution, since it can synthesize a pha random copolyester of 3-hydroxybutyrate and 3-hydroxyhexanoate [p(3hb-co-3hhx)] that is a tough and flexible material compared to polyhydroxybutyrate (phb) hom ... | 2002 | 11976116 |
| in vitro stepwise autoprocessing of the proform of pro-aminopeptidase processing protease from aeromonas caviae t-64. | pa protease (pro-aminopeptidase processing protease) is an extracellular zinc metalloprotease produced by the gram-negative bacterium aeromonas caviae t-64. the 590-amino-acid precursor of pa protease is composed of a putative 19-amino-acid signal sequence, a 165-amino-acid n-terminal propeptide, a 33 kda mature protease domain and an 11 kda c-terminal propeptide. the proform of pa protease, which was produced as inclusion bodies in escherichia coli, was subjected to in vitro refolding. it was r ... | 2002 | 11983417 |
| lateral flagella of aeromonas species are essential for epithelial cell adherence and biofilm formation. | mesophilic aeromonas strains express a single polar flagellum in all culture conditions and produce lateral flagella on solid media. such hyperflagellated cells demonstrate increased adherence. nine lateral flagella genes, lafa-u for aeromonas hydrophila, and four aeromonas caviae genes, lafa1, lafa2, lafb and fliu, were isolated. mutant characterization, nucleotide and n-terminal sequencing demonstrated that the a. hydrophila and a. caviae lateral flagellins were almost identical, but were dist ... | 2002 | 11985716 |
| evaluation of immune functions of rainbow trout (oncorhynchus mykiss)--how can environmental influences be detected? | in fish, the first line of defense against infectious microorganisms is based on a broad range of nonspecific humoral and cellular immune mechanisms ("innate immunity") which without prior specific activation can act in forming a more static barrier (fish shellfish immunol. 10 (2000) 243; dev. comp. immunol. 25 (2001) 827). this natural resistance is normally effective enough to protect fish from infectious diseases until specific immune responses are being induced (fig. 1; dev. comp. immunol. 2 ... | 2002 | 11988361 |
| direct detection of diarrheagenic aeromonas from faeces by polymerase chain reaction (pcr) targeting aerolysin toxin gene. | detection of diarrheagenic aeromonas specific aerolysin toxin (aer) gene by pcr based assay and isolation, identification of diarrhea causing aeromonas from faeces by culture methods were carried out in the division of active surveillance, national institute of cholera and enteric diseases (niced), kolkata, india for a period of 12 months. out of 602 faecal samples collected from patients with acute diarrhea admitted in infectious diseases (id) hospital, kolkata, 68 (11.29%) samples were found t ... | 2001 | 12004918 |
| engineering of ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from fructose and solid-state properties of the copolymer. | recombinant ralstonia eutropha capable of producing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer [p(3hb-co-3hhx)] from fructose was engineered by introduction of genes for crotonyl-coa reductase (ccr) from streptomyces cinnamonensis (ccrsc) and for pha synthase and (r)-specific enoyl-coa hydratase from aeromonas caviae (phac-jac). in this recombinant strain, c6-acyl-coa intermediates were provided via beta-ketothiolase-mediated elongation of butyryl-coa, which was generated from croto ... | 2002 | 12005535 |
| occurrence of staphylococcus and enteropathogens in soft cheese commercialized in the city of rio de janeiro, brazil. | to investigate the presence of staphylococcus aureus, enteropathogenic escherichia coli (epec), aeromonas spp. and yersinia spp. in soft cheese commercialized in rio de janeiro, brazil. | 2002 | 12010558 |
| role of bacteria in the epizootic ulcerative syndrome (eus) of fishes. | bacteriological examination of certain water bodies and fishes carrying eus was carried out. as a whole, 17 species of bacteria were isolated from the investigated water bodies and eus affected fishes. the species of bacteria isolated from fishes are common to those isolated from water. experimental infection trials conducted suggested that aeromonas hydrophila in association with pseudomonas fluorescens, may be playing the role of primary aetiological agent in producing eus in fishes. | 2001 | 12017259 |
| screening of soil bacteria for production of biocleaner. | soil bacteria were studied for the production of biodegradable cleaning agents. among 86 bacterial strains resistant to liquid paraffin, 58 showed hemolytic activity. these strains were cultured, and the supernatant of culture broths was evaluated for cleaning activity against a dirty porcelain tile. potent activity was exhibited in 18 strains. the lowest value of surface tension was obtained from bacillus sp. nkb03 suggesting the presence of a biosurfactant. aeromonas sp. nkb26c and bacillus ce ... | 2002 | 12018259 |
| acquired carbapenem-hydrolyzing beta-lactamases and their genetic support. | carbapenem-hydrolyzing beta-lactamases of several ambler molecular classes have been reported as the source of acquired beta-lactam antibiotic resistance in gram negative bacteria. the metallo-enzymes of ambler class b are the most prevalent enzymes in this case. these clavulanic-acid resistant enzymes have a large spectrum of hydrolysis including penicillins, cephalosporins (third and fourth generations), carbapenems but not monobactams. they are responsible for acquired resistance in several g ... | 2002 | 12022255 |
| starvation survival and viable but nonculturable states in aeromonas hydrophila. | the behavior of aeromonas hydrophila stored at 4 degrees c and 25 degrees c in nutrient-poor filtered sterilized distilled water was investigated. at 4 degrees c, the a. hydrophila population declined below the detection level (0.1 cell ml(-1)) after 7 weeks, whereas the number of cells with intact membrane as determined by the live/dead method decreased only by 1 log unit. although, this response is reminiscent of the so-called vbnc state, the cells could not be resuscitated by an upshift to 25 ... | 2002 | 12023732 |
| first documented case of aeromonas veronii biovar sobria infection in northern europe. | the first scandinavian case of aeromonas veronii biovar sobria infection, an episode of peritonitis in a patient suffering from a cirrhotic liver and an earlier unrecognized appendiceal cancer, is described. | 2002 | 12030401 |
| detection and identification of bacterial pathogens of fish in kidney tissue using terminal restriction fragment length polymorphism (t-rflp) analysis of 16s rrna genes. | we report the application of a nucleic acid-based assay that enables direct detection and identification of bacterial pathogens in fish kidney tissue without the need for bacterial culture. the technique, known as terminal restriction fragment length polymorphism (t-rflp), employs the polymerase chain reaction (pcr) using a primer pair that targets 2 highly conserved regions of the gene that encodes for the 16s small subunit of the bacterial ribosome. each primer is 5' labeled with a different f ... | 2002 | 12033704 |
| the immune responses of common carp, cyprinus carpio l., injected with carp interleukin-1beta gene. | the function of the common carp, cyprinus carpio l., interleukin-1beta (il-1beta) gene was studied by dna injection. to investigate the immune responses to il-1beta, a plasmid construct of cytomegalovirus (cmv)-driven carp il-1beta was injected into the epaxial muscle of carp. il-1beta protein expressed in serum on 1, 3, and 5 days after plasmid injection was quantified by elisa and immunohistochemistry. il1-beta gene injection increased proliferation of the lymphocytes by phytohemagglutinin (ph ... | 2002 | 12034023 |
| correlation of the suicide phenomenon in aeromonas species with virulence and enteropathogenicity. | certain strains of mesophilic aeromonads like a. hydrophila, a. veronii biotype sobria and a. caviae when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. as these strains become nonviable after 24 h, this phenomenon is termed suicide. we investigated suicidal strains of aeromonas species as means of understanding animal virulence and enteropathogenicity. non suicidal strains of a. hydrophila showed and overall 88.8% lethality rate and non ... | 2001 | 12035354 |
| activation of soil respiration and shift of the microbial population balance in soil as a response to lavandula stoechas essential oil. | lavandula stoechas, a native plant of greece, is rich in essential oil and fenchone is its major constituent. we examined the effect of the essential oil and its main constituents on soil metabolism and microbial growth. addition of the essential oil or fenchone to soil samples induced a remarkable increase in soil respiration. this was accompanied by an increase in the soil bacterial population of three orders of magnitude. this sizable population was not qualitatively similar to that of the co ... | 2002 | 12035924 |
| foodborne pathogenic bacteria in prepackaged fresh retail portions of farmed rainbow trout and salmon stored at 3 degrees c. | twelve lots of fresh unskinned fillets of rainbow trout (oncorhynchus mykiss) and 10 lots of fresh sliced salmon (salmo salar) prepacked in trays wrapped with an oxygen-permeable film were obtained immediately after packing from two supermarkets having in-plant facilities for packaging wet fish. during storage at 3 degrees c, listeria innocua was detected in eight lots of trout fillets after 4 days storage. l. monocytogenes was recovered from a single lot also contaminated with l. innocua. initi ... | 2002 | 12038570 |
| antibiotic resistance of aeromonas hydrophila isolated from marketed fish and prawn of south india. | a total of 319 strains of aeromonas hydrophila were isolated from 536 fish and 278 prawns for a 2-year period. all the strains were tested for resistance to 15 antibiotics and 100% of the strains was resistant to methicillin and rifampicin followed by bacitracin and novobiocin (99%). only 3% of the strains exhibited resistance against chloramphenicol. the multiple antibiotic resistance (mar) indexing of a. hydrophila strains showed that all of them originated from high-risk sources. | 2002 | 12038573 |
| synthesis of a novel class of polyhydroxyalkanoates in arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation. | the poly[(r)-3-hydroxyalkanoate] (pha) synthase gene (phac(ac)) of aeromonas caviae fa440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. the modified gene was introduced into arabidopsis thaliana plants by agrobacterium-mediated transformation. the transgenic arabidopsis plant expressed the introduced gene and its protein, and it accumulated pha in its tissues. gas chromatography-mass spectrometry analy ... | 2002 | 12040103 |
| an expression release system for heterogeneous proteins in e.coli mediated by the kil gene. | an expression release system for heterogeneous proteins in escherichia coli mediated by the colicin release gene(kil gene) was constructed. the system is based on the ability of the kil protein to release periplasmic proteins into the growth medium. beta-lactamase, an e.coli periplasmic protein, and prolyl endopeptidase (pep), a periplasmic protein of aeromonas punctata subsp. punctata were used as report proteins. commonly, these two proteins are seldom released into the growth medium. the resu ... | 2001 | 12040395 |
| parasitic and bacterial contamination in collards using effluent from treated domestic wastewater in chiang mai, thailand. | thailand often has inadequate water supply for agriculture during the dry season. the reuse of treated wastewater treatment plants could solve this problem. treatment of domestic wastewater of chiang mai municipality by the aerated lagoon system (al) releases more than 25,000 m3 of treated water everyday. the reuse of wastewater in agriculture is an efficient use of water, especially in tropical countries or in drought zones. the objective of this study is to demonstrate the possibility of using ... | 2001 | 12041598 |
| two independent, light-sensing two-component systems in a filamentous cyanobacterium. | two orfs, cpha and cphb, encoding proteins cpha and cphb with strong similarities to plant phytochromes and to the cyanobacterial phytochrome cph1 of synechocystis sp. pcc 6803 have been identified in the filamentous cyanobacterium calothrix sp. pcc7601. while cpha carries a cysteine within a highly conserved amino-acid sequence motif, to which the chromophore phytochromobilin is covalently bound in plant phytochromes, in cphb this position is changed into a leucine. both orfs are followed by rc ... | 2002 | 12047374 |
| a 45-kda acetylcholinesterase protoxin of aeromonas hydrophila: purification and immunogenicity in fish. | a rabbit antiserum to the 15-kda acetylcholinesterase toxin neutralised the lethal effect of the 15-kda toxin of aeromonas hydrophila when injected into trout. however, immunisation of fish with the 15-kda toxoid failed to induce an antibody response, and a higher molecular mass form of this toxin was purified from the extracellular products with the aim of inducing an immune response in fish. the optimal conditions for production of extracellular products by a. hydrophila strain b32 were studie ... | 2002 | 12052546 |
| aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in bangladesh, and extended description of aeromonas hydrophila subsp. hydrophila (chester 1901) stanier 1943 (approved lists 1980). | a group of 10 aeromonas hydrophila-like strains referred to as group bd-2, which mainly comprised haemolytic and cytotoxic isolates from diarrhoeal children in bangladesh, was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (faflp) and eric-pcr fingerprinting, microplate dna-dna hybridization and extensive phenotypic characterization. comparison of faflp and eric-pcr genotypic profiles indicated that the group bd-2 isolates constituted a sep ... | 2002 | 12054229 |
| nosocomial infections with aeromonas hydrophila from leeches. | the manner in which leeches are maintained before they are used for therapy has not been studied as a factor contributing to nosocomial infections. a 5-year retrospective survey of aeromonas hydrophila nosocomial infections at a hospital in marseille, france, revealed infections in 5 (4.1%) of an estimated 122 patients treated with leeches in the hand surgery unit and 2 (2.4%) of an estimated 85 patients treated with leeches in other hospital units. the retrospective survey showed that the hand ... | 2002 | 12060892 |
| rotavirus and enteric pathogens in infantile diarrhoea in manipal, south india. | the etiology of rotavirus in acute diarrhoeal illness in children 0-5 years of age, admitted to the pediatric wards of kasturba medical college hospital, manipal was studied over a period of 5 years. rotavirus in the faeces detected by latex agglutination test accounted for 19.56% of the diarrhoea with maximum incidence (65%) in the 7-12 months of age group. bacterial aetiological agents continued to play a significant role (69.6%) in diarrhoeal diseases. enteroaggregative e. coli was common in ... | 2002 | 12061671 |
| phenotypic study by numerical taxonomy of strains belonging to the genus aeromonas. | this study was undertaken to cluster and identify a large collection of aeromonas strains. | 2002 | 12067377 |
| novel plasmid-encoded class c beta-lactamase (mox-2) in klebsiella pneumoniae from greece. | klebsiella pneumoniae kol, a clinical strain resistant to various beta-lactams, was isolated from the stools of a patient from greece. this strain harbored a new pi 9.1 plasmid-mediated ampc beta-lactamase with unusually high levels of hydrolytic activity for cefoxitin and cefotetan that we named mox-2. sequencing of bla(mox-2) revealed 93.2, 92.9, 92.7, and 73.1% identities with the deduced amino acid sequences of cmy-8, mox-1, cmy-1, and the ampc beta-lactamase of aeromonas sobria, respectivel ... | 2002 | 12069985 |
| comparative studies on aeromonas strains isolated from lakes balaton (hungary) and fertó/neusiedlersee (hungary). | ecological and comparative taxonomic investigations were carried out on 49 aeromonas strains isolated from water samples of two moderately alkaline lakes of hungary, lake balaton and lake fertó/neusiedlersee together with 3 authentic strains of aeromonas hydrophila. five phena were created at greater than 92% similarity value using the upgma method with the jaccard coefficient. strains isolated from lake balaton were determined as a. hydrophila, while strains originated from lake fertó were iden ... | 2002 | 12073824 |
| numbers and types of microorganisms in vacuum-packed cold-smoked freshwater fish at the retail level. | fifty-four packages (each one belonging to a different lot) of vacuum-packed cold-smoked salmon (30) and trout (24) produced by six spanish smokehouses were obtained at retail level after 3 weeks storage at 2+/-1 degrees c. sensorial, chemical, physicochemical and microbiological characteristics were examined. overall, ph, a(w), salt content in water phase, aerobic plate counts at 30 and 25 degrees c. levels of enterobacteriaceae, lactic acid bacteria (lab), fungi and presumptive aeromonads and ... | 2002 | 12076034 |
| pcr-based assays for the fish pathogen aeromonas salmonicida. i. evaluation of three pcr primer sets for detection and identification. | in an effort to develop a rapid diagnostic test for the fish pathogen aeromonas salmonicida, the performance of 2 polymerase chain reaction (pcr) primer sets (ap and paas) targeting the fish pathogen a. salmonicida and 1 pcr primer set (miy) targeting a. salmonicida subsp. salmonicida were evaluated. initially, the pcr assays were used to screen purified dna extracted from 308 a. salmonicida isolates. the ap and paas pcr tests were demonstrated to be 100% specific for the species a. salmonicida ... | 2002 | 12078981 |
| pcr-based assays for the fish pathogen aeromonas salmonicida. ii. further evaluation and validation of three pcr primer sets with infected fish. | two aeromonas salmonicida-specific polymerase chain reaction (pcr) tests and 1 a. salmonicida subsp. salmonicida-specific pcr test were used to screen salmonid populations that were either overtly or covertly infected with a. salmonicida subsp. salmonicida. it was demonstrated that these pcr assays could be used to replace the biochemical testing currently employed to confirm the identity of a. salmonicida isolates cultured from infected fish. the ap and paas pcr assays were also capable of dire ... | 2002 | 12078982 |
| early cell signaling by the cytotoxic enterotoxin of aeromonas hydrophila in macrophages. | a cytotoxic enterotoxin (act) of aeromonas hydrophila is an important virulence factor with hemolytic, cytotoxic and enterotoxic activities. in this report, we demonstrated act rapidly mobilized calcium from intracellular stores and evoked influx of calcium from the extracellular milieu in macrophages. a direct role of calcium in act-induced prostaglandin (e.g. pge(2)) and tumor necrosis factor alpha (tnf alpha) production was demonstrated in macrophages using a cell-permeable calcium chelator b ... | 2002 | 12079405 |
| characterization of carnobacterium divergens strain 6251 isolated from intestine of arctic charr (salvelinus alpinus l.). | an atypical strain of carnobacterium divergens, strain 6251, was isolated from the small intestine of arctic charr (salvelinus alpinus l.), fed high dietary carbohydrate. this strain showed marked growth inhibitory effects in vitro against the fish pathogens aeromonas salmonicida subsp. salmonicida (furunculosis), vibrio anguillarum (vibriosis) and vibrio viscocus (winter ulcer). the strain is a non-motile gram-positive psychrotrophic rod that lacks both catalase and oxidase, grows at ph 9.1 (ct ... | 2002 | 12086178 |
| high mortality in zebrafish (danio rerio). | a group of 100 adult zebrafish were housed in a new system at a stocking density of 20 fish per tank. four weeks after arrival, 15 fish presented with petechial hemorrhages and ulceration on the surfaces of the skin. samples of the fish were collected for histopathology, fungal culture, and bacterial culture and sensitivity. water samples were analyzed for ph, ammonia, nitrite, and submitted for bacterial and fungal culture. histologically, the epidermis had multiple areas of ulceration and mono ... | 1999 | 12086431 |
| bacterial and fungal aerosols in indoor environment in central and eastern european countries. | studies of indoor bioaerosols conducted in central and eastern european countries, as a result of the scarcity of funding, mostly do not attain the level presented by similar studies in northern america and western europe. for socio-economic reasons, most of the intense studies on indoor bioaerosols in central and eastern european countries were carried out in industrial facilities and have contributed significantly to occupational health science. in contrast, until recently, insufficient of stu ... | 2002 | 12088392 |
| technical-scale production of cyanophycin with recombinant strains of escherichia coli. | by the use of escherichia coli dh1 harboring cpha from synechocystis sp. strain pcc6803, large-scale production of cyanophycin at 30- and 500-liter culture volumes was established. transcription of cpha was controlled by the thermosensitive ci857 repressor, which enabled induction of cpha by a simple temperature shift in the culture fluid. maximum cyanophycin cell content of up to 24% (wt/wt) of cellular dry matter was obtained by induction in the early exponential growth phase and cultivation o ... | 2002 | 12089018 |
| an analysis of human pathogens found in horse/mule manure along the john muir trail in kings canyon and sequoia and yosemite national parks. | to determine the prevalence of microorganisms that are potentially pathogenic for humans in horse/mule manure along the john muir trail (jmt). | 2002 | 12092962 |
| aeromonas hydrophila wound infection in elective surgery. | patients with diabetes mellitus are subject to wound infection with unusual pathogens. a patient who had colonic surgery developed aeromonas hydrophila wound infection, which mainly results from contamination by water or soil. | 2002 | 12096577 |
| high-efficiency expression of prolyl endopeptidase from aeromonas punctata subsp. punctata in escherichia coli. | the open reading frame (orf) of prolyl endopeptidase gene from aeromonas punctata subsp. punctata (appep) was amplified by pcr in three parts. the amplified dna segments were ligated to form the complete appep gene, and then cloned into expression vectors pbl (temperature inducible) and pkkh (iptg inducible), respectively. after induction, the expression amounts of recombinant appep in bl21/pkkh-pep and bl21/pbl-pep were about 30% of the total bacterial proteins, and the enzyme activities were 1 ... | 2000 | 12098799 |
| [species composition and microbial contamination of atmospheric humidity condensate and potable water from the mir water supply systems during mir main missions 4 through 27]. | analyzed were quantitative and qualitative characteristics, and processes of formation and transformation of microflora in the atmospheric humidity condensate (ahc) and potable water (pw) regenerated from the condensate by the long-operating system (srv-k) aboard space station mir. the paper presents data on species composition of microflora of ahc, and pw regenerated by srv-k in the period from 1989 through to 1999. the following microbial species were identified in ahc: alcaligenes (14.8%), ba ... | 2002 | 12098948 |
| a novel dizinc bridged hydroxamate model for hydroxamate inhibited zinc hydrolases. | reaction of zn(oac)(2).2h2o with tmen leads to the formation of [zn(tmen)(oac)2] (i) which reacts with benzohydroxamic acid to form zn(ba)2.h2o (ii) and the novel dizinc hydroxamate bridged complex [zn2(mu-oac)2(oac)(mu-ba)(tmen)] (iii), which may also be prepared by self-assembly and whose structure closely mimics that of the native hydroxamate inhibited aeromonas proteolytica aminopeptidase. | 2002 | 12109086 |
| antimicrobial multiresistance in bacteria isolated from freshwater chilean salmon farms. | the intensive use of antimicrobial agents, mainly oxytetracycline, to prevent and control bacterial pathologies in chilean salmon culture is a frequent practice. a total of 103 gram-negative oxytetracycline-resistant bacteria recovered from various sources of 4 chilean freshwater salmon farms were identified and investigated for their susceptibility patterns to various antibacterial agents, by using an agar disk diffusion method. antibacterial resistance patterns of isolates were not correlated ... | 2002 | 12109474 |
| vector potential of houseflies for the bacterium aeromonas caviae. | houseflies, musca domestica linnaeus (diptera: muscidae), have been implicated as vectors or transporters of numerous gastrointestinal pathogens encountered during feeding and ovipositing on faeces. the putative enteropathogen aeromonas caviae (proteobacteria: aeromonadaceae) may be present in faeces of humans and livestock. recently a. caviae was detected in houseflies by pcr and isolated by culture methods. in this study, we assessed the vector potential of houseflies for a. caviae relative to ... | 2002 | 12109714 |
| properties of recombinant aeromonas punctata prolyl endopeptidase. | the properties of recombinant aeromonas punctata prolyl endopeptidase(appep) were studied using specific substrate and peptides. results show that the optimum catalytic temperature and ph was 34 degrees and 8.4, the stability of the appep was in the range of 4-32 degrees and ph 6.0-10.0, and its k(m) was 0.03 mmol/l based on the z-gly-pro-betana. the appep was not sensitive to pmsf, tlck, tpck, trypsion inhibitor, edta, tetrathionate and some metal ions, but was sensitive to sds and zn(2 ), and ... | 1999 | 12110936 |
| bacterial enteropathogens and factors associated with seasonal episodes of gastroenteritis in nsukka, nigeria. | each year, between april and october, many children of school age and some young adults in nsukka, nigeria suffer from gastroenteritis. the period covers the rainy season in this part of africa, when manured farmland occasionally is flooded. in view of the number of people suffering diarrhoea and occasionally low-grade fever, it became necessary to investigate the nature of the bacterial agents responsible. between april and october (1996-1998), 500 loose or watery stools were collected from pat ... | 2002 | 12113407 |
| cloning and nucleotide sequencing of prolyl endopeptidase gene from aeromonas punctata subsp. punctata. | prolyl endopeptidase activity was found in aeromonas punctata subsp. punctata. the genomic dna was partially digested with ecori and the recovered 8-16 kb dna fragments were inserted into the ecori site of plasmid puc19, and were transformated into escherichia coli dh5alpha. the resulted clones were screened by using benzyloxycarbonyl-gly-pro-beta-naphthylamide, the specific substrate of prolyl endopeptidase and a positive clone was obtained. the 12 kb insertion fragment of recombinant plasmid w ... | 1999 | 12114972 |
| the role of the fatty acid beta-oxidation multienzyme complex from pseudomonas oleovorans in polyhydroxyalkanoate biosynthesis: molecular characterization of the fadba operon from p. oleovorans and of the enoyl-coa hydratase genes phaj from p. oleovorans and pseudomonas putida. | in order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (fadba) in the provision of (r)-3-hydroxyacyl-coa thioesters for medium-chain-length polyhydroxyalkanoate (pha(mcl)) biosynthesis, the fadba(po) operon of pseudomonas oleovorans was cloned and characterized. the fadba(po) operon and a class-ii pha synthase gene of pseudomonas aeruginosa were heterologously co-expressed in escherichia coli to determine whether the putative epimerase funct ... | 2002 | 12115060 |
| diverse bacteria are pathogens of caenorhabditis elegans. | practically and ethically attractive as model systems, invertebrate organisms are increasingly recognized as relevant for the study of bacterial pathogenesis. we show here that the nematode caenorhabditis elegans is susceptible to a surprisingly broad range of bacteria and may constitute a useful model for the study of both pathogens and symbionts. | 2002 | 12117988 |
| characterization of a novel plasmid-mediated cephalosporinase (cmy-9) and its genetic environment in an escherichia coli clinical isolate. | an escherichia coli strain, hkym68, which showed resistance to broad-spectrum cephalosporins was isolated from a sputum specimen in japan. the high-level resistance of the strain to ceftazidime, cefpirome, and moxalactam was carried by a self-transferable plasmid. the beta-lactamase gene responsible for the resistance was cloned and sequenced. the deduced amino acid sequence of this gene product, cmy-9, had a single amino acid substitution (e85d), the residue reported to be part of the recogniti ... | 2002 | 12121914 |
| influence of diet on the occurrence of some bacteria in the intestinal flora of wild and pet birds. | enterobacteriaceae, e. coli in particular, do not belong to the intestinal flora of granivorous pet birds. this is indicated by the fact that the feces of only 9% of healthy budgerigars and 17% of finches tested were positive for enterobacteria. stressful situations such as overcrowding in small cages coincident with increased noise and low light levels can enhance the colonization of the gut with e. coli. on the other hand it seems nearly impossible to colonize the intestine of budgerigars with ... | 2002 | 12125172 |
| optimisation of one-tube pcr-elisa to detect femtogram amounts of genomic dna. | a simple, high-throughput, low-cost polymerase chain reaction-enzyme linked immunosorbent assay (pcr-elisa) protocol that detects the presence of 4 fg of dna from four bacterial fish pathogens yersinia ruckeri, tenacibaculum maritimum (formerly flexibacter maritimus), lactococcus garvieae and aeromonas salmonicida was developed. dna amplification was undertaken in a biphasic system with free and bound pcr that are achieved in the one nucleolink tube. solid-phase amplicons were detected using bio ... | 2002 | 12133608 |
| clinical presentation of microbial keratitis with daily wear frequent-replacement hydrogel lenses: a case series. | microbial keratitis (mk) is a serious complication associated with contact lens wear. in this case series we report three clinical presentations of mk associated with daily-wear monthly-replacement soft contact lenses. all of these patients were using vifilcon a (55% water content, ionic, u.s. food and drug administration [fda] group iv) monthly-replacement soft contact lenses. a gram-stained smear of the corneal scrapings of two patients showed numerous gram-negative cocci bacilli, and tissue c ... | 2002 | 12144239 |
| the occurrence of cytotoxic aeromonas hydrophila strains in italian mineral and thermal waters. | bacteria of the genus aeromonas are ubiquitous in aquatic environments, including mineral drinking and thermal waters. motile species are related to different diseases, mostly gastrointestinal disorders. criteria for aeromonas pathogenicity in humans and animals are still unclear and neither is the relationship between production virulence and pathogenicity factors. in the present study, strains of aeromonas hydrophila, from 61 samples of bottled mineral waters and 23 thermal italian sources hav ... | 2002 | 12146524 |
| rapid detection of six types of bacterial pathogens in marine waters by multiplex pcr. | a rapid multiplex pcr (m-pcr) method that allows the simultaneous detection, in a single tube, of six commonly encountered waterborne pathogens is developed. the target genes used were: the aerolysin (aero) gene of aeromonas hydrophila, the invasion plasmid antigen h (ipah) gene of shigella flexneri, the attachment invasion locus (ail) gene of yersinia enterocolitca, the invasion plasmid antigen b (ipab) gene of salmonella typhimurium, the enterotoxin extracellular secretion protein (epsm) gene ... | 2002 | 12146868 |
| bacterial flora from the gut of the wild and cultured banana prawn, penaeus merguiensis. | there is growing awareness of the influence of the bacterial composition of the gut on the health and growth of the host. this study compared the bacterial flora from the digestive system of the wild and cultured prawn, penaeus merguiensis. | 2002 | 12147069 |
| the role of the n-terminal propeptide of the pro-aminopeptidase processing protease: refolding, processing, and enzyme inhibition. | pro-aminopeptidase processing protease (pa protease) is an extracellular zinc metalloprotease produced by aeromonas caviae t-64 and it is classified as m04.016 according to the merops database. the precursor of pa protease consists of four regions; a signal peptide, an n-terminal propeptide, a c-terminal propeptide, and the mature pa protease. the in vitro refolding of the intermediate pro-pa protease containing the c-terminal propeptide (mc) was investigated in the presence and absence of the n ... | 2002 | 12147230 |
| evaluation of a new etest for detecting metallo-beta-lactamases in routine clinical testing. | several etest (ab biodisk, solna, sweden) gradient formats were developed for detection of metallo-beta-lactamases based on the reduction of imipenem (ip) or ceftazidime (tz) mics in the presence of edta or 2-mercaptopropionic acid (mpa). the etest metallo-beta-lactamase (etest mbl) strips consisted of a double-sided seven-dilution range of ip or tz (4 to 256 microg/ml) and ip or tz (1 to 64 microg/ml) overlaid with a constant concentration of edta or mpa. the prototype strips were evaluated on ... | 2002 | 12149325 |
| a global non-conjugative tet c plasmid, pras3, from aeromonas salmonicida. | two 11.8 kb non-conjugative, but mobilizable r plasmids designated pras3.1 and pras3.2 were isolated from aeromonas salmonicida subspecies salmonicida and atypical a. salmonicida, respectively. differences between the plasmids were of minor extent and they are considered as being variants of the same plasmid, pras3. the genes repa, repb, moba, mobc, mobd, and mobe were organized similar to corresponding genes in the small, mobilizable plasmid ptf-fc2 isolated from acidithiobacillus ferrooxidans ... | 2002 | 12151232 |
| endotoxins associated with cyanobacteria and their removal during drinking water treatment. | the aim of this study was to investigate endotoxin concentrations in cyanobacterial water blooms and strains, and to assess the removal of endotoxins during drinking water treatment. endotoxin concentrations were measured from 151 hepatotoxic, neurotoxic and non-toxic cyanobacterial water blooms by using limulus amebocyte lysate (lal) assay, and the results were compared to bacterial data. endotoxin activities ranged from 20 to 3.8 x 10(4) endotoxin units (eu) per ml. endotoxicity of the samples ... | 2002 | 12153030 |
| physicochemical and biological properties of an extracellular serine protease of aeromonas sobria. | previously, we cloned a protease gene of aeromonas sobria, determined its nucleotide sequence and established a method of purifying its product. in this study, we examined the properties of the purified protease. the protease was temperature-labile and had an optimal ph of 7.5. metallo-protease inhibitors and a cysteine protease inhibitor did not block the proteolytic activity of the enzyme. the treatment with reagents to modify sulfhydryl group did not reduce the activity. but, serine protease ... | 2002 | 12153115 |
| enterotoxin-like factors of aeromonas species isolated in kenya. | three out of thirteen aeromonas strains tested have a heat-labile enterotoxin-like factor which does not cross-react with cholera toxin serologically, but evokes fluid accumulation in the gut of infant mouse, and causes chinese hamster ovary cell elongation. | 1994 | 12153351 |
| leech therapy for patients with surgically unsalvageable venous obstruction after revascularized free tissue transfer. | to assess the efficacy and associated complications of a leech therapy protocol used for patients with a head and neck free tissue transfer in whom flap viability is threatened because of surgically unsalvageable venous obstruction. | 2002 | 12162779 |
| in vivo evolution of the aeromonas punctata polyhydroxyalkanoate (pha) synthase: isolation and characterization of modified pha synthases with enhanced activity. | in vivo random mutagenesis of the polyhydroxyalkanoate (pha) synthase gene from aeromonas punctata was performed employing the mutator strain escherichia coli xl1-red. about 200,000 mutants were screened on nile red-containing medium and five mutants with enhanced fluorescence were selected. four of these mutants exhibited enhanced in vivo and in vitro pha synthase activity. mutant m1, which carried the single mutation f518i, showed a five-fold increase in specific pha synthase activity, whereas ... | 2002 | 12172613 |
| virulence markers in aeromonas hydrophila and aeromonas veronii biovar sobria isolates from freshwater fish and from a diarrhoea case. | to evaluate the public health significance of representative strains of two aeromonas spp., mainly from freshwater fish, on the basis of production of virulence-associated factors and presence of the haemolytic genes aera and hlya. | 2002 | 12174039 |
| biochemical identification and numerical taxonomy of aeromonas spp. isolated from environmental and clinical samples in spain. | to study the phenotypic characteristics of aeromonas spp. from environmental and clinical samples in spain and to cluster these strains by numerical taxonomy. | 2002 | 12174040 |
| the 1.20 a resolution crystal structure of the aminopeptidase from aeromonas proteolytica complexed with tris: a tale of buffer inhibition. | the aminopeptidase from aeromonas proteolytica (aap) is a bridged bimetallic enzyme that removes the n-terminal amino acid from a peptide chain. to fully understand the metal roles in the reaction pathway of aap we have solved the 1.20 a resolution crystal structure of native aap (pdb id = 1lok). the high-quality electron density maps showed a single tris molecule chelated to the active site zn(2+), alternate side chain conformations for some side chains, a sodium ion that mediates a crystal con ... | 2002 | 12176384 |