| characterization of aeromonas and vibrio species isolated from a drinking water reservoir. | to study the phenotypic and chemotaxonomic (i.e. phospholipid and cellular fatty acid composition) characteristics of environmental aeromonas spp. and vibrio spp. isolated from a drinking water reservoir near vladivostok city, and the application of some chemotaxonomic markers for discrimination of the two genera and species. | 2001 | 11412322 |
| prevalence of enterotoxigenic motile aeromonads in children, fish, milk and ice-cream and their public health significance. | motile aeromonads, which have been implicated as causative agents of human gastroenteritis, meningitis. endocarditis and osteomyelitis, were isolated from fish, pasteurized and flavored milk and ice creams. forty-five isolates of aeromonas from diarrheic children (100) and 285 food samples comprising of fish (100), milk (85) and ice creams (100) were identified as a. hydrophila (21), a. sobria (16) and a. caviae (8). five of these 45 isolates of aeromonoas (3 a. sobria, 2 a. hydrophila) were fro ... | 2000 | 11414446 |
| mechanical carrier of bacterial enteric pathogens by chrysomya megacephala (diptera: calliphoridae) in chiang mai, thailand. | chrysomya megacephala was studied regarding its mechanically bacterial carrier in urban areas of chiang mai, northern thailand. fifty-six adult flies were randomly collected using sweep insect net during april-may, 1999 from 3 fresh food markets and examined for bacteriological isolation. among them, 49 flies (87.5%) were bacterial carriers. the total 22 bacterial species and 8 groups were isolated. three species previously reported as the bacterial enteric pathogens causing diarrheal disease we ... | 2000 | 11414447 |
| aerolysin from aeromonas hydrophila and related toxins. | | 2001 | 11417121 |
| immunological parameters of javanese carp puntius gonionotus (bleeker) exposed to copper and challenged with aeromonas hydrophila. | this study was to determine the median lethal concentration (lc50) of copper to javenese carp, puntius gonionotus (bleeker), and the immune response after the fish were exposed to sublethal levels of copper and challenged with formalin killed aeromonas hydrophila. the lc50 of copper on p. gonionotus at 24, 48, 72, 96 and 120 h were estimated as 2.17, 0.91, 0.57, 0.53 and 0.42 mg l(-1), respectively. to determine the effect of copper on the immune system, fish were exposed for 66 days to 0.05, 0. ... | 2001 | 11417716 |
| effect of ascorbic acid on the immune response of the catfish, mystus gulio (hamilton), to different bacterins of aeromonas hydrophila. | ascorbic acid is one of the essential vitamins for normal physiological activities of any organism. the present study demonstrates an immunostimulatory effect of vitamin c on the humoral and cell mediated immunity of the bagrid catfish, mystus gulio, determined using different bacterins of aeromonas hydrophila. humoral as well as cell mediated immune responses were elucidated in the vitamin supplemented, vaccinated and unvaccinated groups. the vitamin supplemented lipopolysaccharide (lps) vaccin ... | 2001 | 11417721 |
| bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards. | a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ... | 2001 | 11417826 |
| detection of rtx toxin gene in vibrio cholerae by pcr. | a pcr that amplifies a recently discovered vibrio cholerae rtx (repeat in toxin) toxin gene was developed. among 166 clinical and environmental isolates of v. cholerae causing epidemics and sporadic cases of cholera in various parts of the world, all were found to be toxigenic by both pcr and hep-2 cell cytotoxicity assay. standard strains of the classical biotype containing a deletion within the gene cluster exhibited negative results by both assays. this is the first rapid genotyping method fo ... | 2001 | 11427575 |
| antibacterial effect of protamine in combination with edta and refrigeration. | the antimicrobial effect of protamine (clupeine) on a range of gram-positive and gram-negative foodborne pathogens and spoilage bacteria, was evaluated using an agar dilution assay and a broth dilution assay with alamar blue as growth indicator. protamine was tested alone at concentrations from 0 to 10,000 microg/ml, and in combination with edta (0.9 mm). assays were performed at 5 degrees c, 10 degrees c, 18 degrees c and 30 degrees c to test the effect of temperature. minimum inhibitory concen ... | 2001 | 11428574 |
| characterisation of volatile compounds produced by bacteria isolated from the spoilage flora of cold-smoked salmon. | this study investigated the volatile compounds produced by bacteria belonging to nine different bacterial groups: lactobacillus sake, l. farciminis, l. alimentarius, carnobacterium piscicola, aeromonas sp., shewanella putrefaciens, brochothrix thermosphacta, photobacterium phosphoreum and enterobacteriaceae isolated from cold-smoked salmon. each bacterial group was represented by several strains. in addition, combinations of the groups were examined as well. sterile blocks of cold-smoked salmon ... | 2001 | 11428576 |
| using light scatter signal to estimate bacterial biovolume by flow cytometry. | in the past decade, flow cytometry has become a useful and precise alternative to microscopic bacterial cell counts in aquatic samples. however, little evidence of its usefulness for the evaluation of bacterial biovolumes has emerged in from the literature. | 2001 | 11429769 |
| the cell division genes (ftse and x) of aeromonas hydrophila and their relationship with opsonophagocytosis. | a transposon mutant from aeromonas hydrophila ah-3 was obtained which was highly resistant to opsonophagocytosis. the mutation was identified in the ftse gene and we characterised the operon ftsy, e and x from this bacterium. these genes, as in enteric bacteria, are neighbours to rpoh. the a. hydrophilia ftse and x genes were fully able to complement escherichia coli ftse mutants, and also complement the opsonophagocytosis-resistant phenotype of the a. hydrophila mutant strain. this phenotype se ... | 2001 | 11430412 |
| the mgte mg2+ transport protein is involved in aeromonas hydrophila adherence. | aeromonas hydrophila ah-3 strains carrying mutations in mgte, which encodes a mg2+ and co2+ transport system, showed a 50% reduction of in vitro adherence to hep-2 cells, a reduction in swarming in semisolid swarming agar, and decrease in biofilm formation of over 60% in comparison to the wild-type strain. the cloned a. hydrophila mgte expressed from a plasmid complements a salmonella typhimurium strain deleted for all mg2+ transporters both phenotypically and by measurement of 57co2+ uptake. li ... | 2001 | 11430413 |
| inactivation of aeromonas hydrophila metallo-beta-lactamase by cephamycins and moxalactam. | incubation of moxalactam and cefoxitin with the aeromonas hydrophila metallo-beta-lactamase cpha leads to enzyme-catalyzed hydrolysis of both compounds and to irreversible inactivation of the enzyme by the reaction products. as shown by electrospray mass spectrometry, the inactivation of cpha by cefoxitin and moxalactam is accompanied by the formation of stable adducts with mass increases of 445 and 111 da, respectively. the single thiol group of the inactivated enzyme is no longer titrable, and ... | 2001 | 11432752 |
| [evaluation of bacterial and polluting loads of effluent from activated sludge wastewater treatment plants in yaounde, cameroon]. | activated sludge wastewater treatment plants in yaounde are plagued by failures in their operation. a microbiological and physico-chemical study was carried out on wastewater effluent of an hospital, university campus, and two residential areas made up of more than 2,000 apartments connected to two collective sewase systems. wastewater sampling was performed in the form of daily cycles during which composite samples were made every two hours with samples collected every half hour. variables meas ... | 2001 | 11440881 |
| case report: recovery of calliphora vicina first-instar larvae from a human traumatic wound associated with a progressive necrotizing bacterial infection. | human myiasis caused by calliphora vicina is rare in europe. here we report a case of c. vicina infection occurring in the traumatic leg wound of a healthy 21-year-old man. firstly, a progressive necrotizing infection developed in the wound despite administration of antibiotics. aeromonas hydrophila was isolated from the wound samples. secondly, during debridement, c. vicina first-instar larvae were isolated from the wound. to our knowledge, this is the first european case of c. vicina wound myi ... | 2001 | 11442211 |
| resistance to antibiotics and biocides among non-fermenting gram-negative bacteria. | to investigate the antibiotic and biocide susceptibilities of clinical isolates of rarely encountered gram-negative, non-fermenting bacteria. | 2001 | 11442564 |
| [lipids of microorganisms from the family vibrionaceae causing diseases in fishes]. | lipid fractional and fatty acid compositions of microorganisms from the genera aeromonas, pseudomonas, and vibrio (the family vibrionaceae), causing diseases of different fish species, were studied. motile aeromonads and vibrios displayed higher relative contents of membrane lipids and oleic acid and lower relative contents of storage lipids compared with immotile aeromonads and pseudomonads, which is connected with the activities of their movements. immotile aeromonads and vibrios exhibited hig ... | 2001 | 11443908 |
| prostacyclin is neither sufficient alone nor necessary to cause pulmonary dysfunction: results from infusions of prostacyclin and antiprostacyclin antibody in porcine septic shock. | this study evaluated whether prostacyclin is a necessary mediator of inflammation in graded bacteremia or is sufficient alone in pathophysiologic concentrations to cause the pulmonary derangement of bacteremic shock. | 2001 | 11445706 |
| production of n-acyl homoserine lactones by gram-negative bacteria isolated from contact lens wearers. | the purpose of this study was to investigate the production of n-acyl-homoserine lactone (ahl) signal molecules in ocular gram-negative bacteria. a total of 91 ocular strains isolated from contact lens adverse response patients and asymptomatic subjects were used in the study. these included acinetobacter, aeromonas hydrophila, escherichia coli, haemophilus influenzae, klebsiella oxytoca, pseudomonas aeruginosa, serratia liquefaciens, serratia marcescens, and stenotrophomonas maltophilia. the bi ... | 2001 | 11446456 |
| simulating the effect of eliminating routine bacterial screening on the negative predictive value of the ontario fish hatchery disease monitoring program. | we determined the impact of eliminating routine screening for aeromonas salmonicida and yersinia ruckeri on the efficacy of the ontario ministry of natural resources (omnr) fish disease monitoring program, using monte carlo simulation. because the main purpose of the program is to prevent transferring infected fish among omnr hatcheries, or to wild fish populations through stocking waterways, the hatchery-level negative predictive value (hnpv) was used as an indicator of monitoring efficacy. the ... | 2001 | 11448498 |
| identification of a plasmid encoding shv-12, tem-1, and a variant of imp-2 metallo-beta-lactamase, imp-8, from a clinical isolate of klebsiella pneumoniae. | a multidrug-resistant plasmid encoding tem-1, shv-12, and a variant of imp-2 metallo-beta-lactamase, designated imp-8, was identified from a clinical isolate of klebsiella pneumoniae. there are four nucleotide differences between bla(imp-2) and bla(imp-8), resulting in two amino acid differences. bla(imp-8) was also found to be carried by an integron-borne gene cassette similar to the bla(imp-2) cassette. | 2001 | 11451699 |
| [the effect of tannery waste products on viability of selected bacteria from activated sludge]. | in this study we have investigated the influence of sludge on the activity of bacteria strains: acinetobacter baumannii, aeromonas hydrophila and pseudomonas cepacia. to our studies we have taken waste materials used in the chromium compound leather tanning process as well as materials from leather fat and waste materials, in general. it was confirmed that 1:20 dilution of chromium tanning leather waste, retanage and leather fat allowed the survivability of bacteria in all three strains. through ... | 2001 | 11452739 |
| tract microflora in saudi patients with cholelithiasis. | to identify the microflora in the gallbladder of patients undergoing laparoscopic cholecystectomy for gallstones, and the antibiotic susceptibility pattern of the isolates, as well as the usefulness of gram staining of bile at the time of operation. | 2001 | 11469952 |
| infectious complications of cirrhosis. | infectious complications in cirrhotic patients can cause severe morbidity and mortality. bacterial infections are estimated to cause up to 25% of deaths in cirrhotic patients. the most frequent are urinary tract infection, spontaneous bacterial peritonitis, respiratory tract infection, and bacteremia. it has been said that cirrhosis is the most common form of acquired immunodeficiency, exceeding even aids. the specific risk factors for infection in cirrhotic patients are low serum albumin, gastr ... | 2001 | 11469997 |
| functional polymer affinity matrix for purifying hexahistidine-tagged recombinant protein. | a functional polyacrylic acid (paa) adsorbent has been prepared for metal chelate affinity chromatography. it has been found to chelate nickel ion ni2+ strongly, and was evaluated for the ability to bind proteins containing neighbouring histidine residues. the principle of the technique was illustrated with aeromonas hydrophila outer membrane protein ompts. dna elements coding for adjacent histidines were fused to the aeromonas hydrophila ompts gene. subsequent expression in e. coli resulted in ... | 2001 | 11471803 |
| immunoglobulin genes and antibody responses in the spotted wolffish (anarhichas minor olafsen). | the spotted wolffish anarhichas minor olafsen is a promising new species in aquaculture in the cold waters of northern norway. in this paper, some basic immunological studies of this marine species are reported. of comparative interest are the cdna sequences of the immunoglobulin transcript and the antibody responses to model antigens. of more practical importance are the humoral immune responses and antibody specificities to potentially pathogenic bacteria. full length cdna clones encoding the ... | 2001 | 11478516 |
| interactions of streptomyces griseus aminopeptidase with amino acid reaction products and their implications toward a catalytic mechanism. | streptomyces griseus aminopeptidase (sgap) is a double-zinc exopeptidase with a high preference toward large hydrophobic amino-terminus residues. it is a monomer of a relatively low molecular weight (30 kda), it is heat stable, it displays a high and efficient catalytic turnover, and its activity is modulated by calcium ions. the small size, high activity, and heat stability make sgap a very attractive enzyme for various biotechnological applications, among which is the processing of recombinant ... | 2001 | 11484227 |
| bacterial causes of aids-associated diarrhea in thailand. | the incidence of bacterial diarrhea in aids patients has increased steadily and has led to enormous medical and public health problems. in this study, the clinical data together with 350 rectal swab samples each from aids patients with diarrhea (apd) and non-aids patients with diarrhea (napd), were collected and examined for bacterial enteropathogens at the bamrasnaradura infectious diseases hospital (bidh), nonthaburi, thailand from may to december 1996. patients were matched by age and sex. th ... | 2001 | 11485080 |
| [determination of aeromonas spp. and pseudomonas spp. in chlorinated water supply]. | | 2001 | 11490893 |
| enzymatic activity of micro-organisms isolated from cork wine stoppers. | the production of enzymes by micro-organisms which are found on vegetal substrates is important due to their ability to decompose cellulose, lignin and other components, which guarantee the integrity of the vegetal cell. the objective of this study was to determine the enzymatic activity of filamentous fungi, yeasts and bacteria, isolated from natural cork stoppers for bottles of still and sparkling wines. suspensions of fungal conidia, yeasts and bacterial cells of micro-organisms were establis ... | 2001 | 11491526 |
| [maturation pathway of hemolysin of aeromonas sobria and the mechanism of action of the hemolysin]. | aeromonas sobria has been recognized as pathogens associated with acute gastroenteritis in both adults and children. the major virulence factor has been proposed to be hemolysin which possesses both hemolytic and enterotoxic activities. mature (bio-active) hemolysin secreted out of cells binds to the target cells of the host and injure the cells. however, hemolysin remained in bacteria can not express such toxicity. it means that the maturation and secretion pathway of hemolysin is closely relat ... | 2001 | 11494595 |
| virulence factors in vibrios and aeromonads isolated from seafood. | thirty-one isolates from seafood, identified as aeromonas hydrophila (7), aeromonas caviae (11), vibrio parahaemolyticus (3), vibrio fluvialis (5), vibrio alginolytictus (3), vibrio metschnikovii (1) and vibrio damsela (1), were tested for possible virulence factors including extracellular hydrolytic enzymes, haemolysins, cytotoxins (vero and hep-2 cells) and adherence ability (hep-2 cells). all the a. hydrophila strains were beta-haemolytic and produced cytotoxins as well as one strain of v. fl ... | 2001 | 11497085 |
| induction of inflammatory cytokines by extracellular products and lps of the fish pathogen aeromonas salmonicida ssp. achromogenes in mice and mouse cell cultures. | balb/c mice, injected i.p. with extracellular products (ecp) of aeromonas salmonicida subsp. achromogenes (asa), displayed symptoms similar to toxic shock syndrome. the ld(50) observed was between 1.5 and 2.0 microg g(-1) and the mice died within 19 h. four inflammatory cytokines were measured in mice receiving sublethal ecp doses. tnf-alpha and il-6 showed a sharp peak in the serum while il-1 beta and il-2 were not detected. when peritoneal macrophages were cultivated in the presence of ecp, as ... | 2001 | 11498248 |
| rapid iodometric detection of aeromonas amylase and its diagnostic significance. | twenty-five aeromonas hydrophila isolates from different sources (food, 13; clinical, 6 and environmental, 6) were studied for the mode of production of amylase and rapid iodometric detection of the enzyme in vitro. all twenty-five of the isolates produced the enzyme constitutively at 37 degrees c. amylase producing ability was not dependent on the source of isolation of aeromonas (f = 0.1069; p > 0.05). using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 1 ... | 2001 | 11502374 |
| microbiological quality of the bivalve pinctada imbricata commercialized in cumaná, venezuela. | the habitual consumption of oysters in the city of cumaná, venezuela, is made in poor sanitary conditions, therefore a study was carried out to determine the sanitary quality of the product. for this, 120 samples of oyster pinctada imbricata were collected directly from: a) fishing boats (unhandled samples) and b) after being shucked by the salesman (handled samples). unhandled samples showed the following mpn/g: faecal coliforms < 3 - 2.2 x 10, aeromonas sp. < 3 - 1.1 x 10(3), staphylococcus au ... | 2001 | 11510429 |
| new dna-dna hybridization and phenotypic data on the species aeromonas ichthiosmia and aeromonas allosaccharophila: a. ichthiosmia schubert et al. 1990 is a later synonym of a. veronii hickman-brenner et al. 1987. | previously, a dna fingerprinting study based on amplified fragment length polymorphism (aflp) analysis has revealed a possible genotypic resemblance of the species aeromonas ichthiosmia and aeromonas allosaccharophila to aeromonas veronii (huys et al., int. j. syst. bacteriol. 46, 572-580 [19961). currently, two genotypically indistinguishable biovars are known to exist in the latter species, namely a. veronii biovar sobria and a. veronii biovar veronii. in the current study, new dna-dna hybridi ... | 2001 | 11518320 |
| identification and characterization of carnobacteria isolated from fish intestine. | eleven bacterial strains were isolated from the gastrointestinal tract of four fish species, atlantic salmon (salmo salar l.), arctic charr (salvelinus alpinus l.), atlantic cod (gadus morhua l.) and wolffish (anarhichas lupus l.). all the strains were gram-positive rods, non-sporing, catalase and oxidase-negative, able to grow at ph 9.0 but not on acetate containing media (ph < or = 5.4), and were fermentative. they had a high content of oleic acid (18:1 n-9) in cellular lipid, and were found t ... | 2001 | 11518321 |
| heat resistance and growth of salmonella enteritidis, listeria monocytogenes and aeromonas hydrophila in whole liquid egg. | salmonella enteritidis atcc,13067, listeria monocytogenes atcc,19116 and aeromonas hydrophila atcc,7965 strains were evaluated for growth and thermal resistance in liquid whole egg (lwe). each strain grew well in lwe at temperatures between 4 and 30 degrees c, except s. enteritidis which grew weakly at 4 and 10 degrees c. maximum populations for each strain increased with increasing growth temperature. the thermal destruction of each strain was determined in six liquid products. the egg products ... | 2001 | 11518391 |
| typing of aeromonas isolates from children with diarrhoea & water samples by randomly amplified polymorphic dna polymerase chain reaction & whole cell protein fingerprinting. | aeromonas spp. are water-borne organisms, often associated with childhood diarrhoea. the present study was conducted to examine the epidemiological relationship among the aeromonas spp. isolated from water and children with acute diarrhoea in chennai. | 2001 | 11525157 |
| induction of apoptosis in ht29 human intestinal epithelial cells by the cytotoxic enterotoxin of aeromonas hydrophila. | the cytotoxic enterotoxin produced by aeromonas hydrophila is considered to be the main virulence factor in gastrointestinal infections mediated by this pathogen. in this study, we examined the morphological and apoptotic effects of this toxin on ht29 cells, using light and electron microscopy in situ, as well as agarose gel electrophoresis of cell dna. cells treated with the cytotoxic enterotoxin became round and lost their polarity as well as their adhesion to each other and to the substrate. ... | 2001 | 11527222 |
| a secreted aminopeptidase of pseudomonas aeruginosa. identification, primary structure, and relationship to other aminopeptidases. | using leucine-p-nitroanilide (leu-pna) as a substrate, we demonstrated aminopeptidase activity in the culture filtrates of several pseudomonas aeruginosa strains. the aminopeptidase was partially purified by deae-cellulose chromatography and found to be heat stable. the apparent molecular mass of the enzyme was approximately 56 kda; hence, it was designated ap(56). heating (70 degrees c) of the partially purified aminopeptidase preparations led to the conversion of ap(56) to a approximately 28-k ... | 2001 | 11533066 |
| regulation of the cytotoxic enterotoxin gene in aeromonas hydrophila: characterization of an iron uptake regulator. | the cytotoxic enterotoxin act from a diarrheal isolate, ssu, of aeromonas hydrophila is aerolysin related and crucial to the pathogenesis of aeromonas infections. to elucidate the role of environmental signals which influence the expression of the cytotoxic enterotoxin gene (act), a portion of the act gene, including the putative promoter region, was fused in frame to a truncated alkaline phosphatase gene (phoa) of escherichia coli. the act::phoa reporter gene was then introduced into the chromo ... | 2001 | 11553581 |
| [aeromonas infections]. | | 2001 | 11555917 |
| an outbreak of acute gastroenteritis due to aeromonas sobria in benghazi, libyan arab jamahiriya. | we report an outbreak of acute diarrhoea due to aeromonas sobria in benghazi which occurred during a 1-month period in 1997. of 69 patients admitted with acute gastroenteritis, 28 were positive for a. sobria based on the production of gas from glucose, the production of acetoin, hydrogen sulfide and lysine decarboxylase and on aesculin hydrolysis and fermentation of arabinose and salicin. the strains were sensitive to chloramphenicol, co-trimoxazole, tetracycline and gentamicin but resistant to ... | 2000 | 11556043 |
| antibody response in salmonids against the 70 kda serine protease of aeromonas salmonicida studied by a monoclonal antibody-based elisa. | an antigen-capture enzyme-linked immunosorbent assay (celisa) based on monoclonal antibodies (mab) was set up and evaluated for selective detection of salmonid antibody responses to the antigen p1, which is a weakly immunogenic exoprotease of typical aeromonas salmonicida. this new assay permits a specific determination of anti-protease-antibodies, without antigen purification. serum antibodies induced by the strongly immunogenic lipopolysaccharide could reliably be discriminated from anti-p1-an ... | 2001 | 11557299 |
| a randomized, blinded, controlled trial investigating the gastrointestinal health effects of drinking water quality. | a double-blinded, randomized, controlled trial was carried out in in melbourne, australia, to determine the contribution of drinking water to gastroenteritis. melbourne is one of the few major cities in the world that draws drinking water from a protected forest catchment with minimal water treatment (chlorination only). six hundred families were randomly allocated to receive either real or sham water treatment units (wtus) installed in their kitchen. real units were designed to remove viruses, ... | 2001 | 11564611 |
| thiomandelic acid, a broad spectrum inhibitor of zinc beta-lactamases: kinetic and spectroscopic studies. | resistance to beta-lactam antibiotics mediated by metallo-beta-lactamases is an increasingly worrying clinical problem. candidate inhibitors include mercaptocarboxylic acids, and we report studies of a simple such compound, thiomandelic acid. a series of 35 analogues were synthesized and examined as metallo-beta-lactamase inhibitors. the k(i) values (bacillus cereus enzyme) are 0.09 microm for r-thiomandelic acid and 1.28 microm for the s-isomer. structure-activity relationships show that the th ... | 2001 | 11564740 |
| type ii secretion by aeromonas salmonicida: evidence for two periplasmic pools of proaerolysin. | aeromonas salmonicida containing the cloned gene for proaerolysin secretes the protein via the type ii secretory pathway. here we show that altering a region near the beginning of aera led to a dramatic increase in the amount of proaerolysin that was produced and that a large amount of the protein was cell associated. all of the cell-associated protein had crossed the cytoplasmic membrane, because the signal sequence had been removed, and all of it was accessible to processing by trypsin during ... | 2001 | 11566995 |
| synthesis and antibacterial activity of pyridyl thioureas and arylthiosemicarbazones. | [n-(2-pyridyl)-n'-(4-(un) substituted] thioureas and (substitutedaryl)thiosemicarbazones were synthesised and evaluated for their antibacterial activity. all aryl thiosemicarbazones showed good activity against aeromonas hydrophilia and salmonella typhimurium. but none of the pyridyl thioureas showed any prominent activity against tested bacteria. | 2001 | 11570220 |
| detection of aeromonas hydrophila in a drinking-water distribution system: a field and pilot study. | a 16-month study was conducted on the presence of aeromonas hydrophila in drinking water in indiana, u.s.a. enumeration was conducted in source water, in various sites within a water treatment plant, and in the distribution system in both bulk water and biofilm, as well as in a simulated (annular reactors) drinking-water distribution system. presumptive aeromonas spp. counts on source waters regularly approached 10(3)-10(4) cfu/100 ml, during summer months and granular activated carbon - filtere ... | 2001 | 11575507 |
| helicobacter pylori and other enteric bacteria in freshwater environments in mexico city. | helicobacter pylori infection is common in the mexican population; however, sources, routes, and risk factors for infection as well as mode of transmission remain unclear. | 2001 | 11578764 |
| is aeromonas hydrophila the dominant motile aeromonas species that causes disease outbreaks in aquaculture production in the zhejiang province of china? | the significance of aeromonas hydrophila in association with disease outbreaks in aquaculture production in the zhejiang province of china was investigated. bacteriological examination of moribund fish and crabs resulted in 95 bacterial isolates: 88 bacterial isolates from fish and 7 isolates from crabs. pcr and traditional biochemical methods were used for identification of a. hydrophila. out of 69 motile aeromonads, 35 isolates were identified as a. hydrophila by biochemical tests. however, 6 ... | 2001 | 11592699 |
| not as simple as just punching a hole. | like a variety of other pathogenic bacteria, aeromonas hydrophila secretes a pore-forming toxin that contribute to its virulence. the last decade has not only increased our knowledge about the structure of this toxin, called aerolysin, but has also shed light on how it interacts with its target cell and how the cell reacts to this stress. whereas pore-forming toxins are generally thought to lead to brutal death by osmotic lysis of the cell, based on what is observed for erythrocytes, recent stud ... | 2001 | 11595627 |
| cloning and biochemical characterization of fox-5, an ampc-type plasmid-encoded beta-lactamase from a new york city klebsiella pneumoniae clinical isolate. | klebsiella pneumoniae 5064, isolated in new york, carried plasmid-mediated resistance to multiple beta-lactams and was unresponsive to clavulanic acid. the beta-lactamase gene responsible for cephalosporin resistance encoded fox-5, with 96 to 97% amino acid identities to other members of the fox family of beta-lactamases. the bla(fox-5) coding region was located next to a transposase gene from the aeromonas salmonicida insertion element isas2. | 2001 | 11600376 |
| growth and survival of clinical vs. environmental species of aeromonas in tap water. | the ability of four species of aeromonas (two of clinical and two of environmental origin) to survive and/or grow in tap water microcosms supplemented with sodium thiosulphate was tested. after bottling, the autochthonous microflora reached 6 x 10(5) cfu ml(-1) after a 5-day incubation period in tap water unfiltered and which was non-autoclaved. in filtered tap water, "ultramicrocells" were detected and final populations of ca. 10(6) cfu ml(-1) after 7 days were obtained. aeromonas was inoculate ... | 2001 | 11603856 |
| [drinking water: use, consume and biological hazard]. | the authors drew the attention to the rising danger of water crisis also in countries usually immune from this problem. the most important causes of these crisis are presented along with some of the most important laws concerning civil use of water. the authors underline the biological risk induced by new aetiologycal agents (i.e. aeromonas hydrophyla, criptococcus, escherichia coli (o:127 h7) found in drinking water too. some of the health problems correlated to the civil use of water are also ... | 1995 | 11623483 |
| epidemiology, clinical course and impact on hospitalization costs of acute diarrhea among hospitalized children in athens, greece. | the aim of this study was to describe the etiology, morbidity and hospitalization costs associated with acute diarrhea among hospitalized children in greece. during 1999, 294 hospitalized children (median age 1 y) with acute diarrhea were prospectively studied. bacterial and viral enteropathogens were detected in 100 (34%) and 37 (12.5%) patients, respectively; 17 (6%) patients had mixed infections. isolated agents included salmonella spp. (43 patients; 15%), rotavirus (32; 11%), campylobacter s ... | 2001 | 11669226 |
| three cases of bacteraemia caused by aeromonas veronii biovar sobria. | septicaemia caused by aeromonas species is a life-threatening condition, arising primarily in immunocompromised patients, which has rarely been reported in scandinavia. herein we describe 3 cases of aeromonas sobria bacteraemia from denmark. all the patients were male and all 3 cases occurred during the summer. two patients had acute leukaemia and hiv infection, respectively, while the third patient had colorectal cancer diagnosed several years later. the clinical presentation in all patients wa ... | 2001 | 11669236 |
| coordination chemistry of the amonabactins, bis(catecholate) siderophores from aeromonas hydrophila(1). | the amonabactins are a series of four bis(catecholate) siderophores isolated from the pathogenic organism, aeromonas hydrophila. as tetradentate ligands, they cannot singly satisfy the octahedral coordination sphere of iron. the solution coordination chemistry of the amonabactins has been elucidated using potentiometric and spectrophotometric titrations, circular dichroism, and mass spectroscopy. they form 2:3 metal:ligand complexes at high ph and excess ligand. their complexation behavior is es ... | 1998 | 11670604 |
| acute diarrhoea in children treated in an outpatient setting in athens, greece. | to describe the epidemiology and aetiology of acute diarrhoea among children treated exclusively in an outpatient setting in greece. | 2001 | 11676518 |
| identification and characterization of the three chitin-binding domains within the multidomain chitinase chi92 from aeromonas hydrophila jp101. | the gene (chi92) encoding the extracellular chitinase of aeromonas hydrophila jp101 has been cloned and expressed in escherichia coli. the mature form of chi92 is an 842-amino-acid (89.830-kda) modular enzyme comprised of a family 18 catalytic domain, an unknown-function region (the a region), and three chitin-binding domains (chbds; chi92-n, chbd(ci), and chbd(cii)). the c-terminally repeated chbds, chbd(ci) and chbd(cii), were grouped into family v of cellulose-binding domains on the basis of ... | 2001 | 11679332 |
| [aeromonas species infection with severe clinical manifestation in okinawa, japan -association with gas gangrene-]. | we experienced two patients having aeromonas species infection with severe clinical manifestations. the one patient was a 15-year-old high school girl student, who had been healthy in her school life, was admitted to the hospital with a sudden onset of left thigh muscle pain and swelling. she subsequently went into septic shock and died one day after admission. pathological examination on autopsy revealed massive gas formation, skin bullas and ulcers, and extensive severe soft tissue damage thro ... | 2001 | 11682002 |
| numerical taxonomy of microorganisms isolated from goat cheese made in chile. | 118 strains of heterotrophic microorganisms were isolated from goat cheese produced domestically in the iv region of northern chile (serene, ovalle, and illapel) and sold in supermarkets in valparaíso, chile. the results of 89 phenotypic tests were numerically analyzed against 17 reference strains, using the simple matching coefficient (s(sm)). thirteen phena were found at a 78% similarity level. five of them (a, b, c, d, and e) were assigned to the family enterobacteriaceae, phenon f was identi ... | 2001 | 11685504 |
| antimicrobial peptide defenses against pathogens associated with global amphibian declines. | global declines of amphibian populations are a source of great concern. several pathogens that can infect the skin have been implicated in the declines. the pathogen most frequently associated with recent die-offs is a chytrid fungus, batrachochytrium dendrobatidis. a second fungus, basidiobolus ranarum, was isolated from declining populations of wyoming toads. a third pathogen, aeromonas hydrophila, is an opportunistic bacterium found in healthy frogs, but capable of inducing disease. among the ... | 2002 | 11687264 |
| industrial scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). | large scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] by aeromonas hydrophila 4ak4 was examined in a 20,000 l fermentor. cells were first grown using glucose as a carbon source, and polyhydroxyalkanoate (pha) biosynthesis was triggered by the addition of lauric acid under conditions of limited nitrogen or phosphorus. when cells first grown in a medium containing 50 g glucose l(-1) were further cultivated after the addition of 50 g lauric acid l(-1) under phosph ... | 2001 | 11693933 |
| immunity induced shortly after dna vaccination of rainbow trout against rhabdoviruses protects against heterologous virus but not against bacterial pathogens. | it was recently reported that dna vaccination of rainbow trout fingerlings against viral hemorrhagic septicaemia virus (vhsv) induced protection within 8 days after intramuscular injection of plasmid dna. in order to analyse the specificity of this early immunity, fish were vaccinated with plasmid dna encoding the vhsv or the infectious haematopoietic necrosis virus (ihnv) glycoprotein genes and later challenged with homologous or heterologous pathogens. challenge experiments revealed that immun ... | 2002 | 11696382 |
| identification of thermophilic and mesophilic bacteria and fungi in esfahan (iran) municipal solid waste compost. | the thermophilic and mesophilic microbiota in compost produced from esfahan municipal solid waste were examined at different stages of composting process from day zero to 28 days and was conducted in four different seasons. some of the mesophilic bacteria observed in initial stages of composting process were gram negative escherichia, klebsiella, aeromonas and alcaligenes, and gram positive enterococcus and bacillus species. after 20 days of the composting process lower species diversity of meso ... | 2001 | 11699859 |
| occurrence of two superoxide dismutases in aeromonas hydrophila: molecular cloning and differential expression of the soda and sodb genes. | aeromonas spp., considered as emerging opportunistic pathogens, belong to the family vibrionaceae. among the criteria currently used for their classification is the presence of a single fesod (iron-containing superoxide dismutase), which distinguishes them from enterobacteriacea. in this paper the cloning of the soda and sodb genes encoding two different sods in aeromonas hydrophila atcc 7966 is reported. the sodb gene encoded an fesod (196 amino acids, 21.5 kda), was constitutively expressed an ... | 2001 | 11700360 |
| bacterial interactions with contact lenses; effects of lens material, lens wear and microbial physiology. | contact lens wear is a successful form of vision correction. however, adverse responses can occur during wear. many of these adverse responses are produced as a consequence of bacterial colonization of the lens. the present study demonstrated that during asymptomatic contact lens wear lenses are colonized by low levels of bacteria with gram-positive bacteria, such as coagulase negative staphylococci, predominating. gram-negative bacteria are frequently the causative agents of adverse responses d ... | 2001 | 11700795 |
| [surveillance of various enteropathogenic bacteria from diarrheal cases during 1989-1999 in kobe city]. | incidence of various enteropathogenic bacteria was examined from diarrheal faecal samples that were collected from the patients of kobe city general hospital and some station hospitals (23,862), and from overseas travelers (2,855) over a period of decade (1989-1999) in kobe. a total of 1,580 strains were isolated from domestic and 331 strains from overseas travelers. the results are as follows. 1) thirteen kinds of enteropathogenic bacteria were isolated from domestic diarrheal cases (6.6%). sal ... | 2001 | 11712364 |
| incidence, distribution, and spread of tetracycline resistance determinants and integron-associated antibiotic resistance genes among motile aeromonads from a fish farming environment. | a collection of 313 motile aeromonads isolated at danish rainbow trout farms was analyzed to identify some of the genes involved in high levels of antimicrobial resistance found in a previous field trial (a. s. schmidt, m. s. bruun, i. dalsgaard, k. pedersen, and j. l. larsen, appl. environ. microbiol. 66:4908-4915, 2000), the predominant resistance phenotype (37%) being a combined oxytetracycline (otc) and sulphadiazine/trimethoprim resistance. combined sulphonamide/trimethoprim resistance (135 ... | 2001 | 11722922 |
| behavior of aeromonas hydrophila in bottled mineral waters. | the growth and survival of aeromonas hydrophila in three types of natural mineral waters were investigated. mineral waters with different levels of mineral content (low, medium, and high) were experimentally contaminated with a. hydrophila, stored at different temperatures (10 degrees c and 20 degrees c), and analyzed at intervals over a 60-day period. water samples that were not experimentally contaminated were investigated for indigenous a. hydrophila. the results confirmed that a. hydrophila ... | 2001 | 11726170 |
| dissimilatory fe(iii) and mn(iv) reduction by shewanella putrefaciens requires fere, a homolog of the pule (gspe) type ii protein secretion gene. | shewanella putrefaciens strain 200 respires anaerobically on a wide range of compounds as the sole terminal electron acceptor, including ferric iron [fe(iii)] and manganese oxide [mn(iv)]. previous studies demonstrated that a 23.3-kb s. putrefaciens wild-type dna fragment conferred metal reduction capability to a set of respiratory mutants with impaired fe(iii) and mn(iv) reduction activities (t. dichristina and e. delong, j. bacteriol. 176:1468-1474, 1994). in the present study, the smallest co ... | 2002 | 11741854 |
| characterization of 13 kda granule-associated protein in aeromonas caviae and biosynthesis of polyhydroxyalkanoates with altered molar composition by recombinant bacteria. | analysis of native poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] inclusions from aeromonas caviae fa440 revealed that orf1 (a 348-bp gene located immediately upstream of phac(ac)) encodes a 13-kda granule-associated protein, which was referred to as phap(ac). several recombinant strains of a. caviae were constructed and conducted to analyze their pha-producing abilities. a transconjugant of fa440 harboring additional copies of phapcj(ac) genes accumulated p(3hb-co-3hhx) copolyes ... | 2001 | 11749166 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by metabolically engineered escherichia coli strains. | recombinant escherichia coli strains harboring a plasmid containing a novel artificial polyhydroxyalkanoate (pha) operon consisting of the aeromonas pha biosynthesis related genes and ralstonia eutropha reductase gene were developed for the production of poly(3-hydroxybutyrate-co-hydroxyhexanoate) [p(3hb-co-3hhx)] from dodecanoic acid. by applying stepwise reduction of dissolved oxygen concentration (doc) during the fermentation, the final dry cell weight, pha concentration, and pha content of 7 ... | 2001 | 11749180 |
| circulating pig-a mutant t lymphocytes in healthy adults and patients with bone marrow failure syndromes. | paroxysmal nocturnal hemoglobinuria (pnh) is a clonal hematological disorder with acquired pig-a gene mutations and absent surface expression of proteins utilizing glycosylphosphatidylinositol (gpi) anchors. pnh often follows aplastic anemia, suggesting pig-a mutant cells have relative dominance over normal hematopoietic cells. somatic pig-a mutations could arise after aplasia, or healthy persons could have rare pig-a mutant cells that expand under selection pressure. | 2001 | 11750098 |
| lateral flagella and swarming motility in aeromonas species. | swarming motility, a flagellum-dependent behavior that allows bacteria to move over solid surfaces, has been implicated in biofilm formation and bacterial virulence. in this study, light and electron microscopic analyses and genetic and functional investigations have shown that at least 50% of aeromonas isolates from the species most commonly associated with diarrheal illness produce lateral flagella which mediate swarming motility. aeromonas lateral flagella were optimally produced when bacteri ... | 2002 | 11751834 |
| bacterial flora of hirudo medicinalis and their antibiotic sensitivities in the middle black sea region, turkey. | the rate of infectious complications of leech therapy is almost 20% because hirudo medicinalis has endosymbiotic bacteria. the aim of this study was to determine the bacterial flora of h. medicinalis and their antibiotic sensitivities in a region in turkey. sixteen adult leeches were collected in middle black sea region, turkey. they were rubbed onto blood agar plates directly under ether anesthesia to obtain surface cultures. they were then killed to obtain mouth and gut cultures. culture swabs ... | 2001 | 11756807 |
| effect of dietary beta-1,3 glucan on immune responses and disease resistance of healthy and aflatoxin b1-induced immunocompromised rohu (labeo rohita hamilton). | the immunostimulant beta-1,3 glucan was fed at 0.1% in feed for 7 days to healthy and aflatoxin b1 (afb1)-induced immunocompromised fish, labeo rohita (one of the major tropical carp species), in a 60 day trial. the effects of afb1, glucan and their interactions on non-specific and specific immunity levels and disease resistance of fish were studied. a single intraperitoneal injection of afb1 at 1.25 mg kg(-1) body weight) caused a significant (p < 0.05) reduction in non-specific immunity as mea ... | 2001 | 11759039 |
| class 1 integrons mediate antibiotic resistance in the fish pathogen aeromonas salmonicida worldwide. | the presence of class 1 integrons was investigated in 38 sulfonamide-resistant strains of aeromonas salmonicida subsp. salmonicida, atypical a. salmonicida and escherichia coli conjugants with r plasmids originating from a. salmonicida. the strains originated from finland, france, japan, norway, scotland, switzerland, and the united states. additional resistance determinants in strains with class 1 integrons were also determined. of 21 strains containing a class 1 integron, 19 had a single gene ... | 2001 | 11759088 |
| the occurrence of enteric pathogens and aeromonas species in organic vegetables. | a range of commercially available organic vegetables (n = 86) was examined for the presence of salmonella, campylobacter, escherichia coli, e. coli o 157. listeria and aeromonas spp., to provide information on the occurrence of such organisms in organic vegetables in northern ireland. the study was not designed to quantify such organisms or to compare occurrence with conventionally farmed vegetables. standard enrichment techniques were used to isolate and identify enteric pathogens and aeromonas ... | 2001 | 11759753 |
| isolation and identification of motile aeromonas species from chicken. | in this study a total of 140 broiler carcasses and carcass parts purchased at different supermarkets in ankara including 50 whole carcass, 30 wing, 30 leg and 30 breast samples were analysed for the presence of motile aeromonas species. according to analysis findings, motile aeromonas spp. were isolated from 116 (82.9%) of total 140 samples. the distribution of the isolates were 94%, 86.6%, 80%, 63.3% in broiler carcass, wing, leg and breast samples, respectively. aeromonas hydrophila was isolat ... | 2001 | 11765602 |
| [clinical characterization of blaimp positive gram-negative rods isolated cases]. | we detected the metallo-beta-lactamase gene blaimp positive strains of the gram-negative rods (gnr) isolated in oita medical university hospital between 1993 and 1999 and studied the clinical characteristics of patients infected or colonized with blaimp positive gnr. 25 strains (20 pseudomonas aeruginosa and 5 serratia marcescens) were detected and most of them were isolated from urinary samples after 1997. in the studies of antimicrobial susceptibility, some strains had sensitivity to aztreonum ... | 2001 | 11766377 |
| effect of high oxygen modified atmosphere packaging on microbial growth and sensorial qualities of fresh-cut produce. | the application of high oxygen atmospheres (hoa) (i.e. > 70% o2) for packaging ready-to-eat vegetables was evaluated as an alternative technique for low o2 equilibrium modified atmosphere (ema) packaging (3% o2-5% co2-balance n2) for respiring products. comparative experiments between both techniques were performed in-vitro and in-vivo. typical spoilage causing microorganisms (pseudomonas fluorescens, candida lambica), the moulds botrytis cinerea, aspergillus flavus and the opportunistic psychro ... | 2001 | 11789938 |
| type ii topoisomerase quinolone resistance-determining regions of aeromonas caviae, a. hydrophila, and a. sobria complexes and mutations associated with quinolone resistance. | most aeromonas strains isolated from two european rivers were previously found to be resistant to nalidixic acid. in order to elucidate the mechanism of this resistance, 20 strains of aeromonas caviae (n = 10), a. hydrophila (n = 5), and a. sobria (n = 5) complexes, including 3 reference strains and 17 environmental isolates, were investigated. fragments of the gyra, gyrb, parc, and pare genes encompassing the quinolone resistance-determining regions (qrdrs) were amplified by pcr and sequenced. ... | 2002 | 11796341 |
| microbiological quality of drinking water at eight water treatment plants. | eight drinking water treatment plants were sampled monthly during one year to evaluate the removal of bacterial indicators, new indicators and some pathogenic bacteria. six plants are allocated along the nile river at cairo segment and the two others on ismailia canal. in this study many parameters were determined; the classical bacterial indicators (total bacterial counts at 22 and 37 degrees c, total coliforms, faecal coliforms and faecal streptococci) show the same trend in all plant intakes ... | 2001 | 11798415 |
| diversity, persistence, and virulence of aeromonas strains isolated from drinking water distribution systems in sweden. | the aeromonas populations in 13 swedish drinking water distribution systems, representing different treatments, were investigated. from each system, water samples were collected four times during the period from may to september 1994 from raw water and water after treatment and at two to five sites within the distribution system. in total, 220 water samples were collected. from samples containing presumptive aeromonas, up to 32 colonies were analyzed by the pheneplate aeromonas (php-ae) system, ... | 1997 | 11799982 |
| detection of aeromonas caviae in the common housefly musca domestica by culture and polymerase chain reaction. | aeromonas caviae has been implicated in diarrhoeal disease of livestock and humans. the potential role of houseflies in the epidemiology of this pathogen was investigated by examining the prevalence of a. caviae in houseflies collected from two south carolina farms and one restaurant. isolation was accomplished by culture of flies in alkaline peptone water followed by identification with aeromonas-specific pcr using novel primers (apw-pcr). all isolates cultured from houseflies were identified a ... | 2001 | 11811891 |
| identification and characterization of pathogenic aeromonas veronii biovar sobria associated with epizootic ulcerative syndrome in fish in bangladesh. | sparse information is available on the virulence factors of aeromonas strains isolated from diseased fish, from the environment, and from humans. in the present study, 52 aeromonas isolates obtained from epizootic ulcerative syndrome (eus) lesions in fish, from the aquatic environment, and from children with diarrhea in bangladesh were identified by biochemical phenotyping (i.e., pheneplate [php] typing) and dna fingerprinting and then characterized with respect to certain putative virulence fac ... | 2002 | 11823203 |
| virulence factors of clinical isolates of aeromonas caviae. | the mechanism of pathogenicity of 13 aeromonas caviae strains isolated from fecal specimens of children with diarrhea was examined. isolates possessed several virulence agents. six of 13 isolates produced cholera-like toxin, all strains exhibited adhesive ability and secreted catecholate siderophores. six isolates were able to grow in heat-inactivated serum. production of hemolysins was not detected. the isolates had relatively high values of ld50 in a mouse model, which suggests that there is n ... | 2001 | 11830944 |
| genomic heterogeneity of environmental and clinical aeromonads. | thirty-three isolates of aeromonas from environmental sources and clinical samples were tested and the results, obtained using the pulsed field gel electrophoresis (pfge) technique, were compared with those obtained by biochemical typing. on the basis of their biochemical characteristics 31 strains was assigned to one of the recognised groups or species within the aeromonas genus and 2 strains to the species vibrio fluvialis. these latter were nevertheless found to belong to the aeromonas genus ... | 2002 | 11837387 |
| diseases, prophylaxis and treatment of the atlantic halibut hippoglossus hippoglossus: a review. | after substantial investments in research, the atlantic halibut hippoglossus hippoglossus is now being cultivated commercially in norway, iceland, scotland and canada. as with other domesticated species, disease problems have been experienced. this review summarizes the current state of knowledge of diseases of the atlantic halibut, and their diagnosis, prophylaxis and treatment. in economic terms, the most important losses have been suffered at the larval and juvenile stages. the most important ... | 2001 | 11843141 |
| distribution of virulence genes in aeromonas spp. isolated from sardinian waters and from patients with diarrhoea. | to characterize 46 isolates of different aeromonas spp. strains (26 aeromonas hydrophila, 13 aeromonas sobria and 7 aeromonas salmonicida) isolated from coastal water and clinical sources in sardinia, italy. | 2002 | 11849349 |
| the cloning and characterization of a second alpha-amylase of a. hydrophila jmp636. | the aim of this study was to identify, clone and characterize the second amylase of aeromonas hydrophila jmp636, amyb, and to compare it to amya. | 2002 | 11849357 |
| structure of peptidase t from salmonella typhimurium. | the structure of peptidase t, or tripeptidase, was determined by multiple wavelength anomalous dispersion (mad) methodology and refined to 2.4 a resolution. peptidase t comprises two domains; a catalytic domain with an active site containing two metal ions, and a smaller domain formed through a long insertion into the catalytic domain. the two metal ions, presumably zinc, are separated by 3.3 a, and are coordinated by five carboxylate and histidine ligands. the molecular surface of the active si ... | 2002 | 11856302 |
| the aminopeptidase from aeromonas proteolytica can function as an esterase. | the aminopeptidase from aeromonas proteolytica (aap) can catalyze the hydrolysis of l-leucine ethyl ester ( l-leu-oet) with a rate of 96 +/- 5 s-1 and a km of 700 microm. the observed turnover number for l-leu-oet hydrolysis by aap is similar to that observed for peptide hydrolysis, which is 67 +/- 5 s-1. the k(cat) values for the hydrolysis of l-leu-oet and l-leucine- p-nitroanilide ( l- pna) catalyzed by aap were determined at different ph values under saturating substrate concentrations. cons ... | 2002 | 11862549 |
| a case of acute cholecystitis due to aeromonas sobria and hafnia alvei from northern europe. | | 1998 | 11864254 |
| transfer of multiple drug resistance plasmids between bacteria of diverse origins in natural microenvironments. | plasmids harboring multiple antimicrobial-resistance determinants (r plasmids) were transferred in simulated natural microenvironments from various bacterial pathogens of human, animal, or fish origin to susceptible strains isolated from a different ecological niche. r plasmids in a strain of the human pathogen vibrio cholerae o1 e1 tor and a bovine escherichia coli strain were conjugated to a susceptible strain of the fish pathogenic bacterium aeromonas salmonicida subsp. salmonicida in marine ... | 1994 | 11865872 |
| temperature dependent activation of leucocyte populations of rainbow trout, oncorhynchus mykiss, after intraperitoneal immunisation with aeromonas salmonicida. | the temperature dependence of in vivo activation of rainbow trout oncorhynchus mykiss, leucocyte populations after intraperitoneal injection (i.p.) of fish with a t-cell independent antigen aeromonas salmonicida (strain mt423) was investigated using a proliferation assay and flow cytometric analysis with mab specific for trout leucocyte surface markers. in trout kept at 15-17 degrees c a prominent activation of blood and spleen leucocytes was found. also, drastic changes of the percentage of the ... | 2002 | 11866129 |