| specificity of the immune response of mice to herpes simplex virus glycoproteins b and d constitutively expressed on l cell lines. | mouse l cell lines have been developed which constitutively express glycoproteins b (gb) and d (gd) of herpes simplex virus type 1. when used to study the immune response of mice to the viral glycoproteins, it was found that both gb and gd induce a delayed type hypersensitivity response and both also induce an antibody response, but only the cell line expressing gd could stimulate the production of neutralizing antibody. virus-specific cytotoxic t lymphocytes (ctls) recognized gb expressed by th ... | 1987 | 3494812 |
| production, binding characteristics, and immunogenicity of heterologous anti-idiotypic antibody to herpes simplex virus glycoprotein c. | a monoclonal antibody specific for glycoprotein c (gc) of herpes simplex virus type 1 (hsv-1) was used to prepare a heterologous anti-idiotypic antibody in rabbits. after absorption with normal mouse immunoglobulin (nms) the anti-idiotypic (anti-id) antibody retained binding activity for moab d4.1, the immunogen. the anti-id (anti-id c) also demonstrated a cross-reactive binding activity, as shown by elisa, for moab d4.2 and moab d4.8 which was specific for glycoprotein d (gd) and glycoprotein b ... | 1987 | 3509672 |
| oral bromovinyldeoxyuridine therapy for herpes simplex and varicella-zoster virus infections in severely immunosuppressed patients: a preliminary clinical trial. | twenty-five patients with haematological diseases were treated orally with the highly potent and selective anti-herpes agent, bromovinyldeoxyuridine (bvdu), in a dosage of 7.5 mg/kg/day (divided over three or four doses a day) for 5 days for an intercurrent mucocutaneous herpesvirus infection. of these 25 patients, 8 were severely granulocytopenic at the time of the viral infection, and 12 recently had undergone bone-marrow transplantation; 5 were under cytotoxic therapy for a lymphoproliferativ ... | 1986 | 3511181 |
| oral acyclovir (zovirax) in herpes simplex dendritic corneal ulceration. | sixty patients with simple dendritic corneal ulceration were randomly assigned to double blind treatment with either acyclovir tablets (400 mg) or acyclovir ophthalmic ointment administered five times daily. there was no significant difference in the proportions of patients healed in either treatment group (88.9% on oral acyclovir and 96.6% on acyclovir ointment). the median healing time was five days in both groups. no systemic or significant local side effects were noted in either treatment gr ... | 1986 | 3521717 |
| adenovirus replication as an in vitro probe for drug sensitivity in human tumors. | the feasibility of using adenovirus 5 as an in vitro probe for chemosensitivity in short-term cultures of human tumors was evaluated using human melanoma cell lines and primary cultures of melanoma biopsies. a convenient immunoperoxidase method was developed for quantitating viral replication 2 days after infection. two different approaches were explored: the host cell reactivation assay (hcr) using drug-treated virus; and the viral capacity assay using drug-treated cells. the hcr assay detected ... | 1986 | 3525182 |
| synthesis of fused glycoprotein d of herpes simplex virus type 1 but not type 2 inhibits escherichia coli hosts. | glycoprotein d from either herpes simplex virus type 1 (gd-1) or type 2 (gd-2) has been expressed in escherichia coli as a series of chimeric proteins. the expression vector used in this study, pjs413, was derived from pbr322 and contains several cloning sites between the lacz promoter-operator and the phage lambda cro gene. plasmids containing fusions between the cro gene, gd-related sequences and lacz was constructed and shown to direct the synthesis of 160-kda proteins. the accumulation of fu ... | 1986 | 3527874 |
| detection of viral antigens in cerebrospinal fluid of rabbits with experimental herpes simplex virus type 1 encephalitis. | we investigated several methods for the rapid diagnosis of herpes simplex virus induced encephalitis in a rabbit model. the corneas of twenty-two rabbits were infected with herpes simplex virus type 1 (hsv-1) and diagnosis of herpes encephalitis was made by virus isolation, immunofluorescent and peroxidase staining of brain biopsies, demonstration of anti-hsv igm in cerebrospinal fluid (csf) and by an indirect enzyme-linked immunosorbent assay (elisa), designed for detection of viral antigens. w ... | 1986 | 3530193 |
| combined antiviral effects of acyclovir or bromovinyldeoxyuridine and human immunoglobulin in herpes simplex virus-infected mice. | suboptimal dosage regimens of antivirals (acyclovir or bromovinyldeoxyuridine) and human immunoglobulin have been combined in the treatment of hairless mice infected with herpes simplex virus type 1. the aim of this study was to establish how late after infection human immunoglobulin could be given to still be effective and for how long would the protective effect last. the combination of acyclovir or bromovinyldeoxyuridine with passive immunization proved additive or even synergistic depending ... | 1987 | 3619435 |
| effects of acyclovir therapy during simultaneous reactivation of latent hsv-1 in rabbits. | acyclovir (acv) therapy with a simultaneous reactivation of latent hsv-1 was evaluated in hsv-1 infected rabbit eyes. when the latently infected rabbits received epinephrine iontophoresis into corneas without acv therapy 100% of eyes shed virus into tear film. the shedding was initiated on the second day of the epinephrine iontophoresis and lasted for an average of 4.6 days. when the rabbits received acv (60 mg/kg body weight) intravenously once daily and topically (5% acv ointment) twice daily ... | 1987 | 3619436 |
| recurrent herpes simplex virus type 1 corneal epithelial lesions after radial keratotomy in the rabbit. | the effects of radial keratotomy (rk) on herpes simplex virus type 1 ocular shedding and recurrent corneal epithelial lesions were assessed for 14 days after rk in rabbits latently infected with strain mckrae. ten latently infected rabbits had rk performed on the right eye. the left eye was used as the control. viral shedding was monitored by ocular tear-film swabs. the recurrent lesions were monitored by slit-lamp examination. in the rk-treated eyes, 15 (11%) of 140 swabs were positive, compare ... | 1987 | 3619747 |
| immunologic modulation of virus-induced pathology in a murine model of acute herpetic retinal necrosis. | unilateral inoculation of herpes simplex virus type 1 (kos strain) into the anterior chamber of balb/c eyes produces an ocular disease with a distinctive differential pattern of retinal pathology. specifically, the retina of the inoculated eye remains histologically intact, whereas the contralateral retina becomes necrotic. we demonstrate that retinal necrosis in opposite uninjected eyes directly correlates with the presence of herpes simplex viral antigens, whereas the intact retinas of virus-i ... | 1987 | 3623838 |
| antiherpesvirus activity of 9-(4-hydroxy-3-hydroxy-methylbut-1-yl)guanine (brl 39123) in cell culture. | the activity of 9-(4-hydroxy-3-hydroxymethylbut-1-yl)guanine (brl 39123) against several herpesviruses was compared with that of acyclovir (acv). in plaque reduction tests with clinical isolates of herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), and varicella-zoster virus, mean 50% inhibitory concentrations (ic50s) (n = number tested) for brl 39123 were 0.4 (n = 17), 1.5 (n = 13), and 3.1 (n = 5) micrograms/ml, respectively. corresponding ic50s for acv were 0.2, 0.6, an ... | 1987 | 3631945 |
| quantitative studies on langerhans cells in mouse corneal epithelium following infection with herpes simplex virus. | dendritic langerhans cells (lcs) were identified in flat-mount preparations of mouse corneal epithelium after staining for atpase activity. they were found predominantly in the limbus, but after inoculating the cornea with hsv1 strain sc16 lc, numbers increased both in the limbus and the central cornea. numbers of lcs reached a maximum on day 8 and if severe keratitis was present remained high at least until day 22. a small but significant increase in lcs was also found in the opposite, uninocul ... | 1987 | 3653284 |
| cytological and immunological study of the aqueous humor in acute retinal necrosis syndrome. | we experienced 4 cases of acute retinal necrosis syndrome, and performed anterior chamber paracentesis as a diagnostic aid in their acute and convalescent phases. the aqueous humor obtained by it was used for measuring antibody titers to herpes group virus and electron microscopic observation. one case showed elevated titer to herpes simplex virus type 1 in the convalescent phase, while the other to varicella-zoster virus, suggesting that these 2 viruses could cause this disease. electron micros ... | 1987 | 3658335 |
| dna sequence responsible for the amplification of adjacent genes. | a 10.3-kb dna fragment in the 5'-flanking region of the rat prolactin (rprl) gene was isolated from f1bgh(1)2c1, a strain of rat pituitary tumor cells (gh cells) that produces prolactin in response to 5-bromodeoxyuridine (brdu). following transfection and integration into genomic dna of recipient mouse l cells, this dna induced amplification of the adjacent thymidine kinase gene from herpes simplex virus type 1 (hsv1tk). we confirmed the ability of this "amplicon" sequence to induce amplificatio ... | 1987 | 3677995 |
| potentiated adherence of sickle erythrocytes to endothelium infected by virus. | systemic viral infection is a known precipitant of vasocclusive crisis in sickle patients, but the mechanism underlying this clinical observation is unknown. in the present studies, human umbilical vein endothelial cells were infected with herpes simplex virus type 1 (hsv) to model systemic viral disease. the already abnormal adherence of sickle erythrocytes to control endothelium is enhanced 1.8 +/- 0.4-fold to hsv-infected endothelium (p less than 0.001). this component of potentiated adherenc ... | 1987 | 3680511 |
| measurement of the stratum corneum drug reservoir to predict the therapeutic efficacy of topical iododeoxyuridine for herpes simplex virus infection. | a rapid, in vivo measurement of the penetration of antiviral compounds into the skin would improve our ability to predict the therapeutic efficacy of topical treatments for herpes simplex virus (hsv) infection. we have studied the concentration of iododeoxyuridine (idu) in the stratum corneum of guinea pig skin by tape stripping at different time points after single and multiple topical doses of the drug. these results were correlated with the efficacy of topical idu against an experimental cuta ... | 1987 | 3680984 |
| high-performance liquid chromatographic analysis of (e)-5-(2-bromovinyl)-2'-deoxyuridine and its metabolites in serum, urine and herpes simplex virus type-1 infected cells. | | 1986 | 3700568 |
| response of dairy calves to vaccinia viruses that express foreign genes. | repeated intradermal inoculations of calves with wild-type vaccinia virus and recombinant vaccinia viruses expressing human hepatitis b virus surface antigen and herpes simplex virus, type 1, glycoprotein d produced characteristic pox lesions at each site of injection. in some instances, calves were inoculated as many as five times at intervals from 4 to 7 weeks. the lesions invariably were more severe after the second inoculation. subsequent inoculations produced a less severe area of redness, ... | 1986 | 3700615 |
| pharmacokinetics and metabolism of e-5-(2-[131i]iodovinyl)-2'-deoxyuridine in dogs. | e-5-(2-iodovinyl)-2'-deoxyuridine (ivdu) is a potent inhibitor of herpes simplex virus type 1 replication in vitro. the selective antiviral activity of ivdu is due to preferential phosphorylation by the herpes simplex virus type 1-encoded thymidine kinase. this selective sequesteration provided the rationale for the development of radioiodinated ivdu as a potential radiopharmaceutical compound for use in noninvasive diagnosis of herpes simplex virus encephalitis. we studied the pharmacokinetics ... | 1986 | 3717935 |
| [plasma exchange and acyclovir in behçet's disease]. | it has been postulated that behçet's disease may be a viral induced vasculitis. in the present study plasma exchanges were applied in an attempt to remove circulating immune complexes and restore cellular immunity. high doses of acyclovir were administered in association with plasma exchanges since it has been shown that herpes simplex virus type 1 might be involved in behçet's vasculitis. seven patients with severe ocular manifestations of behçet's disease were selected for the study. plasma ex ... | 1986 | 3722720 |
| herpes simplex encephalitis in infants. | two cases are reported of severe encephalitis in infants that were caused by a primary infection with herpes simplex virus type 1. both patients presented with focal seizures; the clinical diagnosis was confirmed by the rapid appearance of igm antibody to herpes simplex virus type 1 in cerebrospinal fluid and serum. in spite of the early use of acyclovir, which was administered by the intravenous route, the clinical response was poor and both infants were left with severe neurological impairment ... | 1986 | 3724604 |
| exposure to low-dose ultraviolet radiation suppresses delayed-type hypersensitivity to herpes simplex virus in mice. | ultraviolet b (uvb) radiation is reported to induce a defect in epidermal antigen presentation which leads to specific suppression of the delayed-type hypersensitivity (dth) response to trinitrochlorobenzene. we have used a similar system to examine the murine dth response to herpes simplex virus type 1 (hsv-1). mice irradiated with 96 mj/cm2 uvb on shaved dorsal skin 3 days before s.c. injection of live hsv-1 in the flank showed 54-92% suppressed dth responses to challenge with inactivated viru ... | 1986 | 3745940 |
| potential of bromovinyldeoxyuridine in anticancer chemotherapy. | bromovinyldeoxyuridine (bvdu) is a highly potent and selective antiherpetic agent which offers great potential for the treatment of severe herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) infections in cancer patients. bvdu inhibits the replication of hsv-1 and vzv at a concentration as low as 1-10 ng/ml; and the proliferation of tumor cells transformed with the hsv-1 thymidine kinase gene is even inhibited by bvdu concentrations lower than 1 ng/ml. moreover, bvdu is inhibito ... | 1986 | 3752935 |
| ocular penetration and efficacy of chloroethyldeoxyuridine against herpetic keratouveitis. | topical administration of 0.1% or 1% chloroethyldeoxyuridine eyedrops caused a significant reduction in the severity of experimental herpes simplex iritis and stromal keratitis in rabbits. the healing effect caused by chloroethyldeoxyuridine was comparable to that obtained with 0.1% and 0.5% bromovinylde-oxyuridine eyedrops. the drug levels achieved in the anterior chamber fluid following topical application of 1% eyedrops of [2-14c]cedu, a radiolabelled analogue of chloroethyldeoxyuridine, were ... | 1986 | 3759363 |
| thymidine kinase activity of ocular herpes simplex isolates resistant to iudr therapy. | thymidine kinase (tk) activity was examined in extracts of tk-deficient cells infected with low passage isolates of herpes simplex virus type 1 (hsv-1) obtained from patients with herpetic keratitis. tk activity induced by the virus, relative phosphorylation rates of thymidine and iododeoxyuridine (iudr), and km values for thymidine and for iudr were compared for tk induced by the different viral isolates. four of the five isolates showing iudr resistance in vitro and in vivo also exhibited alte ... | 1986 | 3759371 |
| a novel selective broad-spectrum anti-dna virus agent. | a new compound has been found, (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine ((s)-hpmpa), that has potent and selective activity against a broad spectrum of dna viruses, including herpes simplex virus (types 1 and 2); varicella zoster virus; thymidine kinase-deficient (tk-) mutants of herpes simplex and varicella zoster virus; human cytomegalovirus; phocid, simian, suid, bovid and equid herpesviruses; african swine fever virus; vaccinia virus; and human adenoviruses. it is also active again ... | 1986 | 3762696 |
| nk-sensitivity of virus-carrier target cells. | peripheral blood lymphocytes from selected donors have a higher natural killer (nk) activity against the cells of a human erythroblastoid line (k-562) persistently infected with measles virus or herpes simplex virus type 1 than against their uninfected counterparts. the mechanisms by which persistent infection may enhance the nk activity displayed by lymphocytes are discussed. | 1986 | 3765389 |
| improvement of the bioavailability of the anti-herpes virus agent bvdu by use of 5'-o-alkoxycarbonyl derivatives with increased metabolic stability. | (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) was found by hplc analysis to be rapidly metabolized in mice and in liver homogenates from mouse and man to the antivirally inactive (e)-5-(2-bromovinyl) uracil (bvu) but was comparatively stable in blood from both species. of a series of 5'-o-alkoxycarbonyl derivatives of bvdu, the 5'-o-tert.-butoxycarbonyl derivative (brl 37000) was the most stable in mouse and human blood and liver homogenates, neither its ester bond nor its n-glycosidic linkage bei ... | 1986 | 3793660 |
| role of nk cells in protection of mice against herpes simplex virus-1 infection. | natural killer (nk) cells have been implicated in the recognition and killing of a variety of virus infected target cells in vitro, yet their role in vivo remains uncertain. in these experiments, the role of nk cells in the regulation of resistance to herpes simplex virus-1 (hsv-1) was studied. adult c57bl/6 mice are resistant to hsv-1 (hfem strain), but are rendered highly susceptible by treatment with cyclophosphamide 24 hr prior to infection. in this model, passive transfer of 10(8) normal sp ... | 1987 | 3805719 |
| therapeutic efficacy of liposome-encapsulated ribavirin and muramyl tripeptide in experimental infection with influenza or herpes simplex virus. | large, negatively charged, multilamellar liposomes were examined for their ability to improve the therapeutic activity of the broad-spectrum antiviral agent ribavirin (rib) and the synthetic immunostimulant muramyl tripeptide (mtp-pe) in the treatment of viral pneumonitis. liposome-encapsulated mtp-pe (l-mtp-pe) was superior to free mtp-pe in activating alveolar macrophages and in protecting mice against intranasal challenge with 10 ld50 (50% lethal dose) of herpes simplex virus type 1 (hsv-1). ... | 1987 | 3805775 |
| [hemophagocytic syndrome associated with herpes simplex virus. apropos of a case with a fatal outcome]. | a male patient, aged 23 years, with no notable previous history, was admitted to hospital because of fever, splenomegaly, and pancytopenia. the bone marrow was infiltrated by mature looking histiocytes displaying erythrophagocytosis. other findings included polyclonal hypergammaglobulinemia, elevated transaminases, hypofibrinogenemia, and hypertriglyceridemia. a diagnosis of virus-associated hemophagocytic syndrome was made and virology studies attributed it to herpes simplex virus type 1. thera ... | 1986 | 3808939 |
| possible association of mycoplasma and viral respiratory infections with bacterial meningitis. | the presence of viral infection was evaluated in 160 children older than three months with bacterial meningitis who were admitted to children's medical center or parkland memorial hospital, dallas, tx, between october 1979 and march 1982. results were compared with a single serologic specimen in 138 children without meningitis. a recent history of upper respiratory infection was obtained from 60% of patients, including 10/13 with pneumococcal, 9/16 with meningococcal, and 77/131 with haemophilus ... | 1987 | 3812456 |
| in vitro proliferation of lymphocytes from cyclophosphamide-pretreated mice immunized with antigen mixed with dimethyl dioctadecyl ammonium bromide. | a blastogenesis assay employing lymphocytes from cyclophosphamide-pretreated mice immunized with antigen mixed with the immunopotentiating compound dimethyl dioctadecyl ammonium bromide is described. the model antigen used for determining the assay parameters was inactivated purified measles virus. the optimal time for removal of immunologically primed t cells was 7 days after immunization of mice pretreated 2 days previously with 200 mg of cyclophosphamide/kg. the peak lymphoproliferative respo ... | 1987 | 3819441 |
| adenosine deaminase in herpes simplex virus induced corneal stromal disease. | the development of herpes simplex virus (hsv)-induced disciform stromal disease produced by the intrastromal injection of the re strain of hsv-1 is characterized by concurrent increases in adenosine deaminase (ada) activity and corneal thickness. ada activity increased from 8.6 units/cornea in normal corneas to about 14.1 units and 31.1 units in mock-infected controls and hsv-infected corneas respectively by 15 days postinjection. the molecular weight species of ada in the corneas of hsv-infecte ... | 1987 | 3829699 |
| a primate model for acute and recurrent herpetic keratitis. | twelve squirrel monkeys were inoculated in both eyes with the rodanus strain of herpes simplex virus type 1 (hsv-1) and were examined for the presence of acute epithelial keratitis. all of the eyes developed dendritic keratitis within 72 hours after inoculation. the twelve monkeys plus two additional similarly infected monkeys were also examined for the presence of clinical recurrences of ocular herpes infections and spontaneous shedding of virus in their tears. two of the eyes developed stromal ... | 1987 | 3829703 |
| histopathologic characteristics of two forms of experimental herpes simplex virus retinitis. | herpes simplex virus type 1 (hsv-1) inoculated intracamerally into one anterior chamber of a balb/c mouse produces retinitis in the uninoculated contralateral eye within 7 to 10 days while the retina of the inoculated eye is spared. in sharp contrast, animals receiving hsv type 2 (hsv-2) by the anterior chamber route develop a dramatic retinitis in the inoculated eye by day 7 postinoculation while the retina of the contralateral eye remains uninvolved. histopathologic examination of retinal dest ... | 1987 | 3829704 |
| effect of neuraminidase on fc and c3b receptors on rabbit corneal cells infected with herpes simplex virus. | the effect of neuraminidase on fc receptors (fcr) and c3b receptors (c3br) was studied in epithelial, stromal and endothelial cells of the rabbit cornea infected with type 1 (hsv-1) and type 2 herpes simplex virus (hsv-2) in vitro. fcr were induced on epithelial, stromal and endothelial cells of the rabbit cornea by both hsv-1 and hsv-2, but their activities were not enhanced by neuraminidase. on the other hand, the treatment of hsv-infected corneal cells with neuraminidase resulted in the enhan ... | 1987 | 3829705 |
| photochemical cross-linking of cap binding proteins to eucaryotic mrnas: effect of mrna 5' secondary structure. | we used uv light-induced cross-linking to study the interactions of cap binding proteins with the 5' cap structure of eucaryotic mrnas. thymidine kinase gene (herpes simplex virus type 1) transcripts prepared in vitro using the sp6 rna polymerase transcription system were capped and methylated posttranscriptionally with [alpha-32p]gtp and s-adenosyl-l-methionine to yield cap-labeled transcripts. irradiation of capped transcripts with crude rabbit reticulocyte initiation factors in the presence o ... | 1985 | 3837842 |
| "in vitro" study on human mononuclear phagocytic cells behaviour versus dna and rna viruses. | it is generally accepted that mononuclear phagocytic cells are of crucial importance in defence against viral infections. using a method of "in vitro" differentiation from human blood monocytes to macrophages, the interactions between human monocyte-macrophage with herpes simplex virus type 1 and 2, adenovirus type 17, coxsackie virus b type 4, poliovirus type 1 and echo virus type 2, are reported. in all viruses tested on monocytes, the infection of the cells was very poor according to the data ... | 1985 | 3843076 |
| experimental development of herpes simplex virus infection in hamster buccal pouch. | in the present study, we have developed an intraoral herpes simplex virus type 1 (hsv-1) infection model in the hamster buccal pouch. this animal model could be used for further oral cancer research related to herpes simplex virus infection. | 1985 | 3856800 |
| live recombinant vaccines using genetically engineered vaccinia virus. | | 1985 | 3866514 |
| acyclic analogues of 2'-deoxynucleosides related to 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine as potential antiviral agents. | a series of acyclic analogues of 2'-deoxynucleosides related in structure to 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, 1) have been synthesized and evaluated for antiviral activity against herpes simplex virus type 1 (f strain). additionally, the ability of these analogues to function as substrates for the virus-specified thymidine kinase was examined. phosphorylation by this kinase is essential for antiviral activity. although the acyclic 4-oxopyrimidine nucleosides were substrates for ... | 1985 | 3871860 |
| the effect of prostaglandin e2 on the initial immune response to herpes simplex virus infection. | numerous clinical situations, demonstrated to be associated with reactivation of herpes simplex virus infections, have also been shown to produce increases in local levels of prostaglandins. the current study was initiated to determine if prostaglandins play a role in the immune response and control of herpes simplex virus infections. lymphocytes from volunteers were stimulated with concanavalin a, herpes simplex virus 1, or herpes simplex virus 2 in the presence of prostaglandin e2 or ibuprofen ... | 1985 | 3872073 |
| a role for t lymphocytes in preventing experimental herpes simplex virus type 1-induced retinitis. | we have previously shown that herpes simplex virus type 1 (hsv-1) inoculated into the anterior chamber (ac) of one eye of balb/c mice results in retinal destruction in the opposite eye while retinas in virus-injected eyes are preserved. in the present study, immunodeficient mice (athymic balb/c or normal balb/c which had received either gamma-irradiation [450 r] or cyclophosphamide [150 mg/kg treatment]), demonstrated bilateral retinal destruction upon unilateral ac inoculation of hsv-1. reconst ... | 1985 | 3877027 |
| human monocyte-mediated cytotoxicity against herpes simplex virus-infected cells: activation of cytotoxic monocytes by free and liposome-encapsulated lymphokines. | human peripheral blood monocytes were incubated with free or liposome-encapsulated human lymphokines containing macrophage-activating factor (maf) and tested for their effect on herpes simplex virus (hsv)-infected target cells. activated monocytes lysed allogeneic hsv type 2 (hsv-2)-infected whole human embryo cells and xenogeneic balb/c mouse embryo cells (10e2) without any significant effect on uninfected cells, as measured by release of 51cr from target cells after 18 h of cocultivation. kine ... | 1985 | 3882870 |
| the effect of dimethylnitrosamine on host resistance and immunity. | adult female b6c3f1 mice were injected ip with 0.2 ml phosphate-buffered saline (pbs) only or pbs containing 1.5, 3, or 5 mg dimethylnitrosamine (dmn)/kg body wt daily for 14 days. on day 16, mice were evaluated for changes in immune status as measured by the antibody response to sheep red blood cells (srbcs), blastogenesis to t- and b-cell mitogens, natural killer (nk) cell function, delayed hypersensitivity, and alveolar macrophage (am) bactericidal activity; and for changes in host resistance ... | 1985 | 3883572 |
| cultured human neural cells accumulate a heat-shock protein during acute herpes simplex virus infection. | herpes simplex virus-1 (hsv-1) infection of cultured human neural cells causes the accumulation of a host cell-encoded nuclear protein identified as a 57,000 mol.wt. stress protein by monoclonal antibody ti56. this protein is cell cycle-related in fibroblast, may mediate host cell control during hsv infection and could play a role in the regulation of hsv latency. | 1985 | 3909005 |
| oral acyclovir in herpetic keratitis. | a double blind comparative study of oral acyclovir and acyclovir ophthalmic ointment was carried out on 29 patients with simple herpetic dendritic corneal ulceration. patients were randomly allocated to the study. healing was achieved in all fourteen patients treated with acyclovir ointment, and in fourteen out of fifteen patients treated with oral acyclovir. the mean healing time of 5.6 days was identical for both patient groups. there was no significant systemic or local side effects recorded ... | 1985 | 3909544 |
| recombinant human interferon alpha d in hsv-1 recurrence in the rabbit. | recombinant human interferon alpha subtype d (rifn alpha d) was effective in reducing the shedding of herpes simplex virus type-1 (hsv-1) induced by 6-hydroxydopamine iontophoresis followed by topical epinephrine application in previously infected rabbit corneas. a treatment schedule of rifn alpha d, two drops qid was superior to one drop bid. rifn alpha a also appeared to be effective in reducing viral shedding. rabbits treated with rifn alpha d during two episodes of adrenergically induced hsv ... | 1985 | 3918950 |
| a micropreparation of fluorescein conjugates of immunoglobulin g and fab from serum. | protein a-sepharose cl-4b (pas) was used to isolate rabbit immunoglobulin g from crude anti-herpes simplex virus-1 serum. papain treatment of the pas-bound immunoglobulin g released fab fragments from the solid support, while fc-containing fragments remained bound to pas. pas-immobilized immunoglobulin g was fluoresceinated by reaction with fluorescein isothiocyanate followed by papain cleavage to yield fluorescein-conjugated fab fragments in solution. these fragments retained activity toward he ... | 1985 | 3927771 |
| human alpha and gamma interferon analogs in rabbits with herpetic keratitis. | complete gene synthesis methods have been used to construct analogs of human interferons (ifns): these include a consensus of the known human ifn-alpha s, designated ifn-alpha con1 and a variant of human ifn-gamma, designated ifn-gamma 4a. these interferons, in purified form, were used topically against herpes simplex virus type 1 (hsv-1) induced ocular keratitis in rabbits. eyes pretreated with ifn-alpha con1 had decreased signs of infection and a lower incidence of hsv-1 positive trigeminal ga ... | 1985 | 3928522 |
| recombinant huifn-gamma prevents herpes simplex keratitis in african green monkeys: demonstration of synergism with recombinant huifn-alpha 2. | human interferon gamma (huifn-gamma) was found to prevent herpes-simplex virus (hsv 1) induced keratitis in monkey eyes, when administered topically at concentrations of greater than or equal to 3 x 10(5) reference units/ml. protective efficacy demonstrated with lower concentrations of huifn-gamma in combination with low titers of huifn-alpha provided evidence of synergistic interferon activity in vivo. tolerance problems observed in eyes affected by virus inoculation seem to be attributable to ... | 1985 | 3929044 |
| [early treatment of ocular herpes simplex. apropos of 6 cases of herpetic conjunctivitis]. | six patients with previous history of herpetic keratitis were seen during the 48 first hours after the out set of a conjunctivitis. herpes simplex virus type 1 was isolated in all cases. treated immediately with trifluorothymidine, they healed quickly without developing any corneal lesions. these data suggest the use of early antiviral treatment in patients with recurrent herpetic keratitis, even preventive therapy in at risk patients in well known predisposing conditions. | 1985 | 3938787 |
| incidence of herpes genitalis in northern ireland in 1973-83 and herpes simplex types 1 and 2 isolated in 1982-4. | during 1973 - 83 there was a pronounced rise in the number of new cases of herpes genitalis in northern ireland. this study of 127 patients with herpes genitalis shows that 30% had herpes simplex virus type 1 (hsv 1) and 70% had herpes simplex virus type 2 (hsv 2) infections. of 41 sexual contacts who attended, only seven had herpes genitalis (caused in each case by hsv 2); another patient had a history of genital lesions, and one other of oral lesions, but hsv was not isolated from either patie ... | 1986 | 3949348 |
| ocular toxicity of intravitreal vidarabine solubilized in dimethyl sulfoxide. | intravitreal injections of the antiviral drug vidarabine in doses of 10, 50, 100, and 200 micrograms dissolved in 100% dimethyl sulfoxide (dmso) were administered to the eyes of 12 dutch and new zealand pigmented rabbits to determine ocular toxicity. the eyes were examined one week, one month, and two months after inoculation with slit-lamp biomicroscopy, indirect ophthalmoscopy, electroretinography, and histopathologic examination with light and electron microscopy. no permanent damage to ocula ... | 1986 | 3954645 |
| ocular penetration of (125i)ivdu, a radiolabeled analogue of bromovinyldeoxyuridine. | following topical application of (125)ivdu, the radiolabeled analogue of bromovinyldeoxyuridine ([e]-5-[2-bromovinyl]-2'-deoxyuridine), as 0.5% or 0.3% eyedrops, to rabbits, (125i)ivdu appeared in the anterior chamber fluid at drug levels well above the minimum concentration (0.01 microgram/ml) required for inhibition of herpes simplex virus type 1 replication. these findings are consistent with the efficacy of 0.5% bromovinyldeoxyuridine eyedrops in the topical treatment of herpes simplex uveit ... | 1985 | 3967955 |
| lysis of herpes simplex virus (hsv) infected targets. iv. hsv-induced change in the effector population. | herpes simplex virus type 1 (hsv-1) stimulation of peripheral blood lymphocytes from patients (pbl-p) with both recurrent corneal and oral-facial hsv-1 lesions results in altered natural killer (nk) activity. freshly isolated pbl-p exhibit high lysis of hsv-1 infected allogeneic fibroblasts but low lysis of uninfected fibroblasts. after stimulation with hsv-1, pbl-p exhibit markedly increased lysis of uninfected fibroblasts such that the hsv-1 infected and uninfected fibroblasts are lysed with e ... | 1985 | 3972502 |
| viral meningitis. | viral meningitis is part of the aseptic meningitis syndrome but must be distinguished from bacterial meningitis on the basis of a careful examination of the csf and sound clinical judgment. enteroviruses probably account for the bulk of cases of aseptic meningitis that occur in the united states and which are reported to the centers for disease control each year. the seasonal pattern in the incidence of aseptic meningitis is largely due to the seasonal variation of enteroviral infections. early ... | 1985 | 3990441 |
| [digestive manifestations of the acquired immunodeficiency syndrome (aids): study in 26 patients]. | we studied the gastrointestinal manifestations in 26 cases of aids. the patients belonged to two different epidemiological groups: the first group included thirteen french homosexual men, the second group included 6 haitians, 6 africans and a pakistanian, none of them admit homosexual activity. the clinical manifestations were: chronic watery diarrhea in 17 cases, bloody diarrhea in 2 cases; loss of weight in the 26 cases; dysphagia in five cases; jaundice in one patient (due to kaposi sarcoma o ... | 1985 | 3996815 |
| nucleosides. 133. synthesis of 5-alkenyl-1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)cytosines and related pyrimidine nucleosides as potential antiviral agents. | the synthesis of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)cytosines with a halovinyl or vinyl substituent at c-5 was accomplished from the corresponding 5-iodo (fiac, 1) and/or 5-chloromercuri nucleoside analogues with use of li2pdcl4- and pd(oac)2-mediated coupling reactions. thiation of the benzoylated derivative of the 5-ethyluracil nucleoside 3 followed by s-methylation and then ammonolysis provided 5-ethyl-2'-fluoro-ara-c. 5-ethynyl-2'-fluoro-ara-c (19a) and 5-ethynyl-2'-fluoro-ara-u (19 ... | 1985 | 4009596 |
| herpes simplex virus (type 1) encephalitis: report of a case treated with acyclovir. | | 1985 | 4015432 |
| degradation of cellular mrnas induced by a virion-associated factor during herpes simplex virus infection of vero cells. | we have used northern blot hybridization to study the accumulation of specific cellular mrnas in vero cells infected with herpes simplex virus (hsv) type 1 or type 2. hsv-1 infection decreased the cytoplasmic levels of beta- and gamma-actin, beta-tubulin, and histone h3 and h4 mrnas, though not all at the same rate. hsv-2 infection resulted in a more rapid decrease in actin and histone mrna levels compared with hsv-1 infection. the turnover rate of each type of mrna studied was accelerated in hs ... | 1985 | 4020960 |
| dependence on antibody for induction of chemiluminescence in polymorphonuclear leukocytes by herpes simplex virus. | the induction of luminol-dependent chemiluminescence in rabbit polymorphonuclear leukocytes (pmn) by a stromal keratitis causing strain (re) of herpes simplex virus type 1 (hsv-1) was examined. virus alone and virus infected rabbit corneal cells were unable to stimulate chemiluminescence. however, when the virus or virus infected cells were incubated in the presence of hsv-1 specific immune serum or purified igg, a gradual chemiluminescent response was observed. virus and virus infected cells in ... | 1985 | 4030251 |
| herpes simplex virus type 1 infection of endothelium reduces collagen and fibronectin synthesis. | protein synthesis was assessed in virus-infected and -uninfected cultures of bovine aorta endothelial cells by following the incorporation of [14c]proline into nondialyzable protein and by use of an elisa assay. monolayers were infected at confluency by incubating with herpes simplex virus type i at a multiplicity of infection (ratio of virus to cells) of 1.0, 1 hour prior to the introduction of the [14c]proline. samples were taken from infected and uninfected cultures at various time points pos ... | 1985 | 4033068 |
| synergistic activity of combinations of recombinant human alpha interferon and acyclovir, administered concomitantly and in sequence, against a lethal herpes simplex virus type 1 infection in mice. | the nucleoside analog acyclovir [9-(2-hydroxyethoxymethyl)guanine] and the hybrid recombinant human alpha interferon (rhuifn-alpha a/d) were evaluated in weanling mice for their efficacy alone and in combination against a lethal systemic infection with herpes simplex virus type 1. simultaneous parenteral treatment with combinations of both agents at various doses resulted in a higher percentage of survival than when either agent was administered alone, with a synergistic interaction demonstrated ... | 1985 | 4037769 |
| comparison of topically applied 5-ethyl-2'-deoxyuridine and acyclovir in the treatment of cutaneous herpes simplex virus infection in guinea pigs. | three percent 5-ethyl-2'-deoxyuridine (edu) in an aqueous cream base was compared with 5% acyclovir (acv) in polyethylene glycol ointment and 3% edu in 95% dimethyl sulfoxide (dmso) for efficacy in the topical treatment of an experimental dorsal cutaneous herpes simplex virus type 1 infection in guinea pigs. topical acv treatment reduced the mean lesion number by 15%, the lesion area by 32%, and the lesion virus titer by 60% when compared with measurements at contralateral sites treated with the ... | 1985 | 4037770 |
| evaluation of bromovinyldeoxyuridine-related compounds in the treatment of experimental herpes simplex keratitis. | several newly synthesized compounds, all structurally related to the highly potent and selective antiherpes agent bromovinyldeoxyuridine ( [e]-5-[2-bromovinyl]-2'-deoxyuridine), have been evaluated for their healing effect on herpes simplex virus type 1 keratitis in rabbits. these novel compounds included chloroethyldeoxyuridine (5-[2-chloroethyl]-2'-deoxyuridine), trifluoropropenyldeoxyuridine ( [e]-5-[3,3,3-trifluoro-1-propenyl]-2'-deoxyuridine), bromovinylaminodideoxyuridine ( [e]-5-[2-bromov ... | 1985 | 4038133 |
| [herpes simplex virus infections]. | there are two serological types of herpes simplex virus, hsv-1 and hsv-2. the types can be differentiated by different methods. after a primary infection hsv reaches the sensory ganglions by the way of peripheral nervous tracts. the virus remains for life in the ganglion as virus genome. after activation and return movement a new infection can appear. a lot of various diseases are caused by primary infection, reactivation or reinfection. the diagnosis in herpes virus diseases includes the detect ... | 1985 | 4039869 |
| spontaneous electrical activity induced by herpes virus infection in rat sensory neuron cultures. | dissociated cultures of rat dorsal root ganglion neurons were infected with a syncytial strain of herpes simplex virus type 1. over 90% of neurons in infected cultures were spontaneously active and fired action potentials which, on membrane potential hyperpolarization, were replaced by depolarizing events similar to excitatory postsynaptic potentials. amplitude analysis of these events produced populations described by the sum of several unitary events with gaussian rather than binomial or poiss ... | 1985 | 4041799 |
| 5-(haloalkyl)-2'-deoxyuridines: a novel type of potent antiviral nucleoside analogue. | syntheses of 5-(2-haloethyl)-2'-deoxyuridines, 5-(3-chloropropyl)-2'-deoxyuridines, and 5-(2-chloroethyl)-2'-deoxycytidine are described. the antiviral activities of these compounds were determined in cell culture against herpes simplex virus types 1 and 2. all compounds were shown to possess significant and selective antiviral activity. the most potent derivative, 5-(2-chloroethyl)-2'-deoxyuridine (cedu), inhibited hsv-1 at concentrations below 0.1 microgram/ml. it exerted measurable inhibitory ... | 1985 | 4067994 |
| psychosis and herpes simplex encephalitis. | we have reported an unusual presentation of herpes simplex encephalitis in a patient with a 3 1/2-year history of a schizo-affective disorder. in the month immediately before diagnosis, the patient lost contact with reality and became violent. after successful treatment with antipsychotic medication, he had agitation and disorientation, as well as fever and cerebrospinal fluid pleocytosis. there were no focal neurologic findings. when a low-density lesion in the right temporal lobe was defined b ... | 1985 | 4071143 |
| influence of 1-dodecylazacycloheptan-2-one (azone) on the topical therapy of cutaneous herpes simplex virus type 1 infection in hairless mice with 2',3'-di-o-acetyl-9-beta-d-arabinofuranosyladenine and 5'-o-valeryl-9-beta-d-arabinofuranosyladenine. | the predictive value of a recently developed physical model was tested in the topical treatment of cutaneous infections caused by herpes simplex virus type 1 in hairless mice with two ester prodrugs of 9-beta-d-arabinofuranosyladenine (ara-a) (1). the tests were conducted with 2',3'-di-o-acetyl-ara-a (4) and 5'-o-valeryl-ara-a (3) topically applied with and without 15% 1-dodecylazacycloheptan-2-one (2) (azone), a percutaneous penetration enhancer. in addition to the in vivo studies, in vitro dif ... | 1985 | 4087174 |
| type specific and type common antigens in cells infected with herpes simplex virus type 1 and on the surfaces of naked and enveloped particles of the virus. | | 1974 | 4132223 |
| letter: herpes-simplex virus (type 1) transmitted to a neonate. | | 1974 | 4141028 |
| adaptation of herpes simplex virus type 1 to supraoptimal temperature. ii. immunofluorescent and cytological study of virus-infected cells, adapted and non-adapted to 40 degrees c. | | 1974 | 4156156 |
| [herpes simplex virus type 1 and 2 in various disease enities]. | | 1967 | 4294718 |
| [genetic markers of herpes simplex virus. 1. some genetic markers of k strain of herpes simplex virus of the 2d antigenic group]. | | 1970 | 4319411 |
| antigens of herpes simplex virus type 1 and 2-immunodiffusion and inhibition passive hemagglutination studies. | | 1971 | 4326538 |
| size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. | studies of the size, composition, and structure of the deoxyribonucleic acid (dna) of the f and g prototypes of herpes simplex virus (hsv) subtypes 1 and 2 (hsv-1 and hsv-2) showed the following. (i) as previously reported by good-heart et al. hsv-1 and hsv-2 dna have a buoyant density of 1.726 and 1.728 g/cm(3), corresponding to 67 and 69 guanine +/- cytosine moles per cent, respectively. the difference in guanine plus cytosine content of the dna species was confirmed by the finding of a 1 c di ... | 1971 | 4329966 |
| type-specific surface antigens of cells infected with herpes simplex virus (1 and 2). | | 1971 | 4331091 |
| the differentiation of herpes simplex virus type 1 and type 2 by temperature markers. | | 1971 | 4331810 |
| differences in thermal stability of deoxythymidine kinase activity in extracts from cell infected with herpes simplex virus type 1 or type 2. | | 1971 | 4332984 |
| genetic relatedness of type 1 and type 2 herpes simplex viruses. | the extent of homology between herpes simplex virus(1) and(2) (hsv-1 and hsv-2) deoxyribonucleic acid (dna) was measured in two ways: (i) by determination of the relative rate of hybridization of labeled hsv-1 and hsv-2 dna to excess unlabeled hsv-1 or hsv-2 dna immobilized on filters and (ii) by determination of the rate of hybridization of labeled hsv-1 and hsv-2 dna to excess unlabeled hsv-1 or hsv-2 dna in solution. approximately 40% of hsv-1 and hsv-2 dna is homologous at hybridization temp ... | 1972 | 4337161 |
| adenovirus-associated virus multiplication. vii. helper requirement for viral deoxyribonucleic acid and ribonucleic acid synthesis. | the adsorption of adenovirus-associated virus (aav) type 2 by kb cells and the subsequent penetration of the aav genome to the cell nucleus was measured with and without helper adenovirus type 2 (ad2). it was found that the helper virus did not enhance either process. on the other hand, a synthesis of aav deoxyribonucleic acid (dna) or ribonucleic acid (rna) was not detected by nucleic acid hybridization after kb cells were infected with aav type 2 alone, whereas both aav dna and rna synthesis w ... | 1972 | 4339193 |
| adenovirus-associated virus multiplication. 8. analysis of in vivo transcription induced by complete or partial helper viruses. | we have analyzed ribonucleic acid (rna) synthesized in kb cells co-infected with adenovirus-associated virus (aav) type 2 and either adenovirus type 2 (ad2) or herpes simplex virus type 1 (hsv-1). with either type of helper virus, synthesis of aav rna was readily detected by deoxyribonucleic acid (dna)-rna hybridization. as is the case for aav rna synthesized with helper ad2, the aav rna synthesized with hsv-1 as helper annealed only to the thymidine-rich (minus) aav dna strand. in addition, aav ... | 1972 | 4339201 |
| differentiation between herpes simplex virus type 1 and type 2 strains by immunoelectroosmophoresis. | a method has been elaborated to differentiate between herpes simplex type 1 and type 2 viruses by immunoelectroosmophoresis. with rabbit immune sera cross-absorbed with heterologous virus antigen, a distinct difference was shown between the two virus types. herpes simplex type 1 virus tested against cross-absorbed type 1 antiserum gave two precipitin lines. herpes simplex type 2 virus gave one precipitin line when tested against cross-absorbed homologous serum. when the viral antigens were teste ... | 1972 | 4341522 |
| ribonucleic acid synthesis in cells infected with herpes simplex virus: controls of transcription and of rna abundance. | analysis of the kinetics of hybridization in liquid of labeled herpes simplex virus-1 dna and excess viral rna revealed the following: (i) cells infected by herpes simplex virus-1 for 2 hr (before dna synthesis) contain two classes of rna molecules differing 140-fold in molar concentrations. the abundant and scarce rnas are transcribed from 14 and 30% of the dna, respectively. rna extracted at 8 hr after infection (late rna) also contains abundant and scarce classes differing 40-fold in molar co ... | 1972 | 4341703 |
| biologic and immunologic comparison of two plaque variants of herpes simplex virus type 1. | | 1972 | 4343373 |
| proteins specified by herpes simplex virus. 8. characterization and composition of multiple capsid forms of subtypes 1 and 2. | two classes of herpesvirus capsids, designated a and b, were isolated from the nuclei of human cells infected with herpes simplex virus (hsv). a and b capsids share in common four structural proteins, i.e., no. 5, 19, 23, and 24. b capsids contain 7.7 to 9.7 times more deoxyribonucleic acid than a capsids; moreover, they contain proteins no. 21 and 22a in addition. all of the proteins contained in the capsid except no. 22a are present in the enveloped nucleocapsids (virions) in approximately the ... | 1972 | 4344252 |
| differential effect of temperature on the replication of herpes simplex virus type 1 and type 2. | | 1972 | 4344946 |
| immunologic manipulation of metastases due to herpesvirus transformed cells. | herpes simplex virus type 1 (hsv-1), type 2 (hsv-2), and simian virus 40 (sv40) fail to induce immunity in weanling syrian hamsters to transplant of hamster cells transformed by hsv-2. however, the development of metastatic tumors is markedly enhanced by prior immunization with hsv-1. immunization with sv40, ultraviolet-irradiated tumor cells, or ultraviolet-irradiated normal hamster embryo cells inhibits the development of metastases. the hsv-hamster system appears a good one for the study of d ... | 1973 | 4347994 |
| genetic studies with herpes simplex virus type 1. the isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. | | 1973 | 4348796 |
| rna synthesis in cells infected with herpes simplex virus. vii. control of transcription and of transcript abundancies of unique and common sequences of herpes simplex virus 1 and 2. | analysis of the kinetics of hybridization in liquid of labeled herpes simplex virus (hsv) 1 and 2 dnas with excess unlabeled rna extracted at 2 (early) and 8 (late) h postinfection revealed the following. (i) the rna transcripts present in the hsv-1-infected cells at 2 and 8 h postinfection are complementary to 44 and 48% of hsv-1 dna. the rna transcripts present in the hsv-2-infected cells at 2 and 8 h postinfection are complementary to 21 and 50% of hsv-2 dna. (ii) the transcripts present in 2 ... | 1973 | 4351458 |
| antigenic relationships among four herpesviruses. | common viral antigens were detected, by fluorescent-antibody studies, in cells infected with herpes simplex virus 1, squirrel monkey herpesvirus 1, bovine rhinotracheitis, and equine abortion viruses. the two primate viruses showed slight cross-neutralization. | 1973 | 4351969 |
| nonvirion antigens produced by herpes simplex viruses 1 and 2. | of nine herpes simplex virus 1 strains (from lip, mouth, throat, cornea, or brain) only five produced enough nonvirion antigen (i.e., not a structural component of the virus) to be detected by complement fixation with specially prepared, virion-absorbed, type-1 guinea pig antisera, while the remaining four strains produced only enough of the same antigen to induce specific antibody in hyperimmunized guinea pigs. while the type 1 virion antiserum used reacted equally well by complement fixation w ... | 1973 | 4352219 |
| infection with herpes-simplex viruses 1 and 2. ii. | herpes-simplex virus 1 (hsv1) and 2 can cause recurrent infection in man and in nonhuman species localized on a specific area of the body, such as face, cornea, or genitals. the origin of the virus responsible for a recurrent infection is not usually apparent. some possible sources of virus can be exogenous infection, endogenous infection from another site of the body, chronic, continuous viral multiplication around the site of involvement, and persistence of the virus at the site of the recur ... | 1973 | 4353782 |
| oncogenic transformation of hamster embryo cells after exposure to inactivated herpes simplex virus type 1. | the in vitro transformation of hamster embryo fibroblasts by herpes simplex virus type 1 (hsv-1) after exposure of the virus to uv irradiation is described. cell transformation was induced by 2 out of 12 strains of hsv-1 that were tested for transforming potential. cells transformed by the kos strain of hsv-1 were not oncogenic when injected into newborn syrian hamsters. however, cells transformed by hsv-1 strain 14-012 induced tumors in 47% of the newborn hamsters injected. hsv-specific antigen ... | 1973 | 4355928 |
| proteins specified by herpes simplex virus. xi. identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell. | analyses of polypeptides made in hep-2 cells infected with herpes simplex virus type 1 by high-resolution polyacrylamide gel electrophoresis revealed the synthesis of at least 49 infected cell polypeptides (icp) ranging in molecular weight from 15,000 to 280,000. evidence for virus specificity based on increased rates of synthesis postinfection, immunological specificity, and viral control of mobility and rate of synthesis was available for 47 of the icp. these 47 polypeptides can account for 75 ... | 1973 | 4357511 |
| virus type-specific thymidine kinase in cells biochemically transformed by herpes simplex virus types 1 and 2. | transformation of mouse cells (ltk(-)) and human cells (hela bu) from a thymidine kinase (tk)-minus to a tk(+) phenotype (herpes simplex virus [hsv]-transformed cells) has been induced by infection with ultraviolet-irradiated hsv type 2 (hsv-2), as well as by hsv type 1 (hsv-1). medium containing methotrexate, thymidine, adenine, guanosine, and glycine was used to select for cells able to utilize exogenous thymidine. we have determined the kinetics of thermal inactivation of tk from cells lytica ... | 1974 | 4359421 |