| molecular mechanisms of virulence in fungus-host interactions for aspergillus fumigatus and candida albicans. | research on fungi that cause opportunistic infections has increased dramatically during the past few years, largely because these organisms cause significant morbidity and mortality. most of this research has focused on defining the virulence factors produced by these pathogens, as well as developing methods for the diagnosis of fungal diseases. with regard to studies on the biology of candida albicans, it is now possible to isolate genes, disrupt their expression, and observe the specific effec ... | 1998 | 9988513 |
| molecular diagnosis and epidemiology of fungal infections. | a variety of methods are utilized for dna strain subtyping of candida spp. because no 'gold standard' exists. random amplified polymorphic dna (rapd) or restriction enzyme analysis (rea) are useful to determine the source of an outbreak, but more reproducible and discriminatory methods such as southern hybridization and pulsed field gel electrophoresis (pfge) may be required. when applied to some nosocomial candida infections, multiple strains and species have been identified. microevolution of ... | 1998 | 9988514 |
| combination of three typing methods for the molecular epidemiology of aspergillus fumigatus infections. european research group on biotype and genotype of aspergillus. | this study investigated the source of infection and strain relatedness of aspergillus fumigatus isolates from bronchial colonisation and invasive aspergillosis (ia) in four transplant patients. environmental isolates from the patient's home and from the hospital and infecting isolates were obtained for patient a who developed ia. clinic environmental and colonising isolates were obtained for patient b. sequential isolates were obtained from various organs from patient c who developed ia and also ... | 1999 | 9989647 |
| in vitro susceptibilities of aspergillus species to voriconazole, itraconazole, and amphotericin b. | we studied the in vitro activity of voriconazole (vcz) itraconazole (itz) and amphotericin b (amb) against 216 clinical isolates of aspergillus spp. (142 aspergillus fumigatus and 74 nonfumigatus aspergillus spp. isolates) using a broth macrodilution method. the mics (microgram/ml) (mean, range) for a. fumigatus were: vcz 0.88, 0.25-4; itz 0.54, 0.25-4; amb 2.16, 0.5-8. mic90s were: vcz 2, itz 1, amb 4. mics for nonfumigatus aspergillus spp. were: vcz 1.57, 0.25-4; itz 1.74, 0.25-4; amb 2.88, 0. ... | 1999 | 9990469 |
| association of a myosin immunoanalogue with cell envelopes of aspergillus fumigatus conidia and its participation in swelling and germination. | a myosin immunoanalogue was identified in conidia of aspergillus fumigatus by western blotting, indirect immunofluorescence assay, and gold immunoelectron microscopy with two different antimyosin antibodies. the distribution pattern of this protein was followed during the early stages of germination. a single 180-kda polypeptide, detected predominantly in a cell envelope extract, was found to cross-react with monoclonal and polyclonal antibodies raised against vertebrate muscle myosin. immunoele ... | 1999 | 10024566 |
| allergic bronchopulmonary aspergillosis in cystic fibrosis: role of atopy and response to itraconazole. | (1) to determine the relationship between ige levels and the prevalence of allergic bronchopulmonary aspergillosis (abpa) in cystic fibrosis (cf) patients, (2) to establish the usefulness of assessing atopy as an identifying risk factor for abpa, (3) to evaluate the clinical course of patients receiving and not receiving itraconazole as reflected in oral steroid dose requirements and number of acute episodes of abpa, and (4) to determine the role of acute episodes of abpa in pulmonary exacerbati ... | 1999 | 10027433 |
| expression of atrc - encoding a novel member of the atp binding cassette transporter family in aspergillus nidulans - is sensitive to cycloheximide. | a new member of the abc superfamily of transmembrane proteins in aspergillus nidulans has been cloned and characterized. the topology of conserved motifs subgroups atrc in the p-glycoprotein cluster of abc permeases, the members of this subfamily, are known to participate in multidrug resistance (mdr) in diverse organisms. alignment results display significant amino acid similarity to afumdr1 and aflmdr1 from aspergillus fumigatus and flavus, respectively. northern analysis reveals that atrc mrn ... | 1999 | 10036328 |
| in vitro and in vivo activities of ns-718, a new lipid nanosphere incorporating amphotericin b, against aspergillus fumigatus. | we evaluated the in vitro and in vivo potencies of a new lipid nanosphere that incorporates amphotericin b (amb), ns-718, against aspergillus fumigatus. the in vitro activity of ns-718 (the mic at which 90% of strains are inhibited [mic90], 0.25 microgram/ml) against 18 isolates of a. fumigatus was similar to that of deoxycholate amb (d-amb; fungizone; mic90, 0.25 microgram/ml), but ns-718 was more potent than liposomal amb (l-amb; ambi-some; mic90, 1.0 microgram/ml). the in vivo efficacy of ns- ... | 1999 | 10049253 |
| amphotericin b- and fluconazole-resistant candida spp., aspergillus fumigatus, and other newly emerging pathogenic fungi are susceptible to basic antifungal peptides. | the present study shows that a number of basic antifungal peptides, including human salivary histatin 5, a designed histatin analog designated dhvar4, and a peptide from frog skin, pgla, are active against amphotericin b-resistant candida albicans, candida krusei, and aspergillus fumigatus strains and against a fluconazole-resistant candida glabrata isolate. | 1999 | 10049295 |
| fungal peritonitis in patients on continuous ambulatory peritoneal dialysis. | the purpose of this study was to analyze the microbiological and clinical features of fungal peritonitis in patients with endstage renal failure treated with continuous ambulatory peritoneal dialysis (capd). the diagnosis of peritonitis was based on abdominal discomfort or pain, cloudy peritoneal effluent with an elevated leukocyte count and isolation of fungi from the peritoneal effluent. amphotericin b, flucytosine, ketoconazole, miconazole and more recently fluconazole were used for antifunga ... | 1998 | 10052546 |
| early diagnosis of central nervous system aspergillosis with combination use of cerebral diffusion-weighted echo-planar magnetic resonance image and polymerase chain reaction of cerebrospinal fluid. | we treated a patient diagnosed as central nervous system (cns) aspergillosis with the combined use of cerebral diffusion-weighted echo-planar magnetic resonance imaging (dwi) and polymerase chain reaction of the cerebrospinal fluid (csf-pcr). dwi, a cutting-edge imaging modality to reveal the earliest changes of cerebral infarction, detected cerebral fungal embolization when the conventional computed tomographic scan and magnetic resonance imaging failed to reveal it. csf-pcr demonstrated the pr ... | 1999 | 10052742 |
| in-vitro activity of voriconazole, itraconazole and amphotericin b against filamentous fungi. | the in-vitro fungistatic and fungicidal activities of voriconazole were compared with those of itraconazole and amphotericin b. mics for 110 isolates belonging to 11 species of filamentous fungi were determined by a broth microdilution adaptation of the method recommended by the national committee for clinical laboratory standards. minimum lethal concentrations (mlcs) of the three antifungal agents were also determined. the mic ranges of the three compounds were comparable for aspergillus flavus ... | 1998 | 10052897 |
| bronchoscopic and serologic diagnosis of aspergillus fumigatus pulmonary infection in a bottlenose dolphin (tursiops truncatus). | a 4-yr-old male bottlenose dolphin (tursiops truncatus) developed an aspergillus fumigatus pneumonia. fungal elements were identified by cytology and microbiology from endoscopic bronchoalveolar lavage and brushings of a raised yellow endobronchial lesion. the results of qualitative immunodiffusion serology, a technique that identifies specific circulating antibodies to aspergillus fumigatus, were suggestive of an active infection. the dolphin was treated with itraconazole for over 2 yr, which r ... | 1998 | 10065855 |
| comparison of interferon-gamma, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor for priming leukocyte-mediated hyphal damage of opportunistic fungal pathogens. | proinflammatory cytokines have been proposed as adjunctive therapeutic agents to enhance the host immune response during infections caused by opportunistic fungi. the study compared the differential in vitro priming effects of interferon-gamma (ifn-gamma), granulocyte colony-stimulating factor (g-csf), and granulocyte-macrophage colony-stimulating factor (gm-csf) on hyphal damage of opportunistic fungi mediated by isolated neutrophils (polymorphonuclear leukocytes, pmnl) and buffy coat cells (po ... | 1999 | 10068606 |
| deficiency of the hematopoietic cell-specific rho family gtpase rac2 is characterized by abnormalities in neutrophil function and host defense. | in mammals, the rho family gtpase rac2 is restricted in expression to hematopoietic cells, where it is coexpressed with rac1. rac2-deficient mice were created to define the physiological requirement for two near-identical rac proteins in hematopoietic cells. rac2-/- neutrophils displayed significant defects in chemotaxis, in shear-dependent l-selectin-mediated capture on the endothelial substrate glycam-1, and in both f-actin generation and p38 and, unexpectedly, p42/p44 map kinase activation in ... | 1999 | 10072071 |
| allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa) is a syndrome seen in patients with asthma and cystic fibrosis. it is characterized by chronic colonization of the airways with a ubiquitous fungus, aspergillus fumigatus. the clinical expression of abpa results from the complex interaction of chronic colonization of the airways with a fumigatus, host factors allowing this colonization, and the host's genetically determined immune response. clinically the syndrome is characterized by recurrent episo ... | 1999 | 10073280 |
| aspergillus meningitis: diagnosis by non-culture-based microbiological methods and management. | the performance of antibody detection, antigen detection, and aspergillus genus-specific pcr for diagnosing aspergillus meningitis was investigated with 26 cerebrospinal fluid (csf) samples obtained from a single patient with proven infection caused by aspergillus fumigatus. immunoglobulin g antibodies directed against aspergillus were not detected by enzyme-linked immunosorbent assay in csf or serum. the antigen galactomannan was detected in the csf 45 days before a culture became positive, and ... | 1999 | 10074549 |
| contaminations occurring in fungal pcr assays. | successful in vitro amplification of fungal dna in clinical specimens has been reported recently. in a collaboration among five european centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal pcr were analyzed. the identities of all contaminants were specified by cycle sequencing and genbank analysis. twelve of 150 pcr assays that together included over 2,800 samples were found to be contaminated (3.3% of the negative controls were ... | 1999 | 10074553 |
| production of specific monoclonal antibodies to aspergillus species and their use in immunohistochemical identification of aspergillosis. | two anti-aspergillus murine monoclonal antibodies (mabs), designated 164g and 611f, have been produced; both specifically recognize cytoplasmic antigens of a. fumigatus, a. flavus, and a. niger by enzyme-linked immunosorbent assay. the mabs can identify aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining. | 1999 | 10074559 |
| detection of pcr-amplified fungal dna by using a pcr-elisa system. | in order to speed up and standardize polymerase chain reaction (pcr)-based detection of medically important fungi in clinical samples we established a combination of commercially available kits for dna extraction, pcr amplification and detection of the amplicons. the pcr plate assay proved to be as sensitive and specific as our previously published assay (5 cfu ml(-1) blood). moreover, in a selected group of patients, all patients with proven and probable invasive fungal infection were found to ... | 1998 | 10075496 |
| activated lymphocytes reduce adherence of aspergillus fumigatus. | lymphocytes comprise up to 30% of the cells present in human bronchoalveolar lavage fluid and thus could participate in host response to infectious aspergillus fumigatus conidia. we have examined the possibility that lymphocytes might play a role during early infection by either damaging the fungus or interfering with adherence. when incubated with a. fumigatus conidia for 20 h, highly purified 5-day-old lymphocytes activated with either il-2 or phytohaemagglutinin, but not untreated lymphocytes ... | 1998 | 10075497 |
| comparison of six extraction techniques for isolation of dna from filamentous fungi. | filamentous fungi have a sturdy cell wall which is resistant to the usual dna extraction procedures. we determined the dna extraction procedure with the greatest yield of high quality fungal dna and the least predilection for cross-contamination of equipment between specimens. each of six extraction methods was performed using aspergillus fumigatus hyphae. the six methods were: (1) glass bead pulverization with vortexing; (2) grinding with mortar and pestle followed by glass bead pulverization; ... | 1998 | 10075499 |
| analysis of peptidogalactomannans from the mycelial surface of aspergillus fumigatus. | peptidogalactomannans (pgms) from mycelium of two strains of aspergillus fumigatus were fractionated by cetavlon precipitation and size exclusion chromatography and their carbohydrate structures analysed using methylation-fragmentation analysis, partial acetolysis and 13c-nuclear magnetic resonance spectroscopy. the most significant difference between the pgms of the two strains was the degree of branching and the proportion of non-reducing ends of alpha-d-manp and beta-d-galf units. methylation ... | 1998 | 10075501 |
| aspergillus fumigatus antigen exposure results in pulmonary airway resistance in wild-type but not in il-4 knockout mice. | inhalation of aspergillus fumigatus, a ubiquitous fungus, results in the development of allergic bronchopulmonary aspergillosis, a disabling allergic lung disease. for better patient management early diagnosis is essential, and understanding of the immune mechanism is important in achieving this goal. although animal model studies have contributed to the understanding of the disease mechanism, details on the immunopathogenesis are still lacking. in the present study, we have developed an allergi ... | 1999 | 10075870 |
| aspergillus fumigatus catalases: cloning of an aspergillus nidulans catalase b homologue and evidence for at least three catalases. | the presence of catalases in the water soluble fractions of three aspergillus fumigatus strains was investigated using non-denaturing and denaturing polyacrylamide gel electrophoresis and western analysis. using non-denaturing polyacrylamide gel electrophoresis and staining for catalase activity, three separate catalases were identified. an a. fumigatus catalase gene (catb) was cloned from genomic dna using the aspergillus niger catr gene as a probe. polyclonal antibodies were raised to a glutat ... | 1999 | 10076909 |
| [acute hantavirus infection caused by a genetically newly identified viral strain. severe and complicated course of hemorrhagic fever with renal syndrome (nephropathia epidemica)]. | a 49-year-old patient, a hobby hunter, fell ill acutely with joint and limb pain, abdominal pain, nausea and subfebrile temperatures. at hospitalization, the patient was in bad general condition, showing ascites and lid edema, and acute renal failure was diagnosed. | 1999 | 10081288 |
| molecular cloning, characterization, and expression of the m antigen of histoplasma capsulatum. | the major diagnostic antigens of histoplasma capsulatum are the h and m antigens, pluripotent glycoproteins that elicit both humoral and t-cell-mediated immune responses. these antigens may play a role in the pathogenesis of histoplasmosis. m antigen is considered immunodominant because antibodies against it are the first precipitins to arise in acute histoplasmosis and are commonly present during all phases of infection. the biological activity of monomolecular m antigen and its ability to elic ... | 1999 | 10085041 |
| [gerog fresenius and the species aspergillus fumigatus]. | the species aspergillus fumigatus was first extensively described by g. fresenius. j. b. georg w. fresenius was born in frankfurt/main, germany, in 1808 and also died there in 1866. he studied medicine and finished his doctorate thesis (md) in 1829. afterwards he started his career as a physician and surgeon in frankfurt/main in the same year. in 1831 fresenius became a university lecturer for botany at the "senckenbergisches medicinisches institut"; this institute specialized in botany. in this ... | 1998 | 10085696 |
| clinical and microbiological characteristics of smokers with early onset periodontitis. | cigarette smoking is a potential risk factor which has recently been associated with periodontal disease progression. the objective of this study was to compare the microbial profile of smokers and non-smokers in a group of patients with early onset periodontitis. the study population consisted of 60 healthy individuals, 40 males and 20 females aged 22 to 35 yr, exhibiting early onset periodontitis. thirty patients were smokers (30.9 cigarettes/d) and 30 non-smokers. smokers had a higher proport ... | 1999 | 10086883 |
| increased antigen-specific th-2 response in allergic bronchopulmonary aspergillosis (abpa) in patients with cystic fibrosis. | the majority of patients with cystic fibrosis (cf) become colonized with aspergillus fumigatus (a. fumigatus) in the lower respiratory tract, the prevalence being up to 60%. between 1-11% of cf patients develop allergic bronchopulmonary aspergillosis (abpa). previous studies of abpa in selected patients suffering from cystic fibrosis or atopic asthma have suggested a pathogenic role for antigen-specific "th2-like" t lymphocytes. the aim of this study was to evaluate the quantitative importance o ... | 1999 | 10088929 |
| formation of an aspergilloma in a patient with allergic bronchopulmonary aspergillosis on corticosteroid therapy. | | 1998 | 10091467 |
| pulmonary surfactant protein a binds to cryptococcus neoformans without promoting phagocytosis. | evidence is accumulating that the alveolar collecting surfactant protein a (sp-a) plays an important role in the first line of defence against infiltrating pathogenic micro-organisms and viruses. the ability of sp-a to facilitate the binding and uptake of acapsular cryptococcus neoformans by monocyte-derived macrophages, human alveolar macrophages, monocytes and polymorphonuclear leucocytes was investigated. | 1999 | 10092994 |
| an expression system matures: a highly efficient and cost-effective process for phytase production by recombinant strains of hansenula polymorpha. | an efficient process was developed for the low-cost production of phytases using hansenula polymorpha. glucose or glucose syrups, previously reported as repressive substrates, were used as main carbon sources during fermentation. glucose was even the most productive substrate for high-level production of phytases. compared with the process using glycerol, the standard carbon source used for this process until now, the use of glucose led to a reduction of more than 80% in the raw materials costs. ... | 1999 | 10099617 |
| mycotoxins of aspergillus fumigatus in pure culture and in native bioaerosols from compost facilities. | exposure to secondary metabolites of airborne fungi in waste handling facilities is discussed in regard to potential toxic impacts on human health. the relevance of mycotoxins has been intensely studied in connection with contamination of food and feed. toxic secondary metabolites are expected to be present in airborne spores, but exposure to mycotoxins in bioaerosols has not been studied sufficiently. aspergillus fumigatus is one of the most frequent species in the air of compost plants. a wide ... | 1999 | 10101846 |
| an aureobasidin a resistance gene isolated from aspergillus is a homolog of yeast aur1, a gene responsible for inositol phosphorylceramide (ipc) synthase activity. | the aur1 gene of saccharomyces cerevisiae, mutations in which confer resistance to the antibiotic aureobasidin a, is necessary for inositol phosphorylceramide (ipc) synthase activity. we report the molecular cloning and characterization of the aspergillus nidulans aura gene, which is homologous to aur1. a single point mutation in the aura gene of a. nidulans confers a high level of resistance to aureobasidin a. the a. nidulans aura gene was used to identify its homologs in other aspergillus spec ... | 1999 | 10102364 |
| spinal aspergillus abscess in a patient with bronchocentric granulomatosis. | aspergillus fumigatus hyphae is often found in the lung tissue of patients with bronchocentric granulomatosis (bcg). this organism is believed to be one agent responsible for inciting the hypersensitivity response and subsequent development of the characteristic pathology that defines bcg. the definitive etiology of this disease, however, remains conjectural. corticosteroids represent the mainstay of therapy. the fungi recovered from patients with bcg are considered noninvasive; thus, the risk o ... | 1995 | 10155170 |
| modulation of neutrophil-mediated activity against the pseudohyphal form of candida albicans by granulocyte colony-stimulating factor (g-csf) administered in vivo. | renewed interest in neutrophil transfusions has emerged with the development and clinical use of granulocyte colony-stimulating factor (g-csf). g-csf not only increases neutrophil (polymorphonuclear leukocyte, pmnl) production but also modulates various physiological properties of pmnl. the effects of g-csf on pmnl-mediated fungicidal activity were evaluated by administration of g-csf (300 micrograms/day subcutaneously) to 5 healthy volunteers for 6 days. g-csf significantly enhanced pmnl-mediat ... | 1999 | 10191242 |
| synthesis and reaction of cyanopyridone derivatives and their potential biological activities. | 4-carboxy-3-cyano-6-biphenyl-pyrid-2-one (1) was prepared and then allowed to react with methyliodide, benzenesulphonyl chloride, phenylisothiocyanate, acetic anhydride, o-phenylenediamine, phenylmagnesium bromide and phosphorus pentasulphide affording the corresponding n-substituted pyrid-2-one 2-5, 4-(benzimidazol-2-yl)-pyrid-2-one 7, 2-hydroxy-2-phenyl-1,2-dihydro-pyridine 8 and 2-thiopyridone 9 derivatives, respectively. treatment of 1 with dimethyl sulphate and phosphorus oxychloride to giv ... | 1999 | 10192104 |
| effects of aspergillus fumigatus culture filtrate on antifungal activity of human phagocytes in vitro. | aspergillus fumigatus can colonise the airways and the lungs with localised underlying conditions and occasionally invade the surrounding lung tissues even in subjects without systemic predisposing factors, presumably by escaping the local host defences. the aim of this study was to investigate the effects of a fumigatus culture filtrate (acf) on the activities of human phagocytes--inhibition of germination of a fumigatus spores by alveolar macrophages (ams) and hyphal damage by polymorphonuclea ... | 1998 | 10193398 |
| aspergillus fumigatus and aspergillosis. | aspergillus fumigatus is one of the most ubiquitous of the airborne saprophytic fungi. humans and animals constantly inhale numerous conidia of this fungus. the conidia are normally eliminated in the immunocompetent host by innate immune mechanisms, and aspergilloma and allergic bronchopulmonary aspergillosis, uncommon clinical syndromes, are the only infections observed in such hosts. thus, a. fumigatus was considered for years to be a weak pathogen. with increases in the number of immunosuppre ... | 1999 | 10194462 |
| molecular cloning of a second phospholipase b gene, caplb2 from candida albicans. | accumulating evidence suggests that phospholipase b, secreted by pathogenic fungi such as candida albicans, cryptococcus neoformans and aspergillus fumigatus, functions as one of the virulence factors. in the present study, we have attempted to clone phospholipase b gene from c. albicans. by rt-pcr analysis with degenerate primers based on conserved regions of phospholipase b from saccharomyces cerevisiae, penicillium notatum and torulaspora delbrueckii two similar but different cdna fragments w ... | 1999 | 10200936 |
| rapid extraction of fungal dna from clinical samples for pcr amplification. | a hexadecyltrimethylammonium bromide (ctab) method for isolating fungal dna from clinical samples, suitable for pcr amplification is described. yeast and filamentous fungi dna from clinical samples was amplified with primers complementary to the genes coding for rrna, amplifying a 105 bp fragment and internal transcribed spacer primers amplifying fragments between 242 and 622 bp. the level of sensitivity was 10 +/- 5 yeast and 28 aspergillus fumigatus cfu ml-1 of biological fluid. | 1999 | 10200937 |
| [systemic aspergillosis]. | | 1999 | 10201207 |
| [cerebral aspergillosis]. | | 1999 | 10201208 |
| [pulmonary aspergilloma]. | | 1999 | 10201209 |
| [invasive pulmonary aspergillosis]. | | 1999 | 10201210 |
| [allergic bronchopulmonary aspergillosis]. | | 1999 | 10201212 |
| [hepatic aspergillosis]. | | 1999 | 10201213 |
| comparison of e-test and broth microdilution methods for antifungal drug susceptibility testing of molds. | we compared the e test with a broth microdilution method, performed according to national committee for clinical laboratory standards document m27-a guidelines, for determining the in vitro susceptibilities of 90 isolates of pathogenic molds (10 absidia corymbifera, 10 aspergillus flavus, 10 aspergillus fumigatus, 10 aspergillus niger, 10 aspergillus terreus, 10 exophiala dermatitidis, 10 fusarium solani, 10 scedosporium apiospermum, 5 scedosporium prolificans, and 5 scopulariopsis brevicaulis). ... | 1999 | 10203509 |
| femur osteomyelitis due to a mixed fungal infection in a previously healthy man. | we describe a previously healthy, 22-year-old man who, after a closed fracture of the femur and subsequent operation, developed chronic osteomyelitis. within a few days, infected bone fragments, bone, and wound drainage repeatedly yielded three different filamentous fungi: aspergillus fumigatus, aspergillus flavus, and chalara ellisii. histologic examination of the bone revealed septate hyphae. after sequential necrotomies of the femur and irrigation-suction drainage with added antimycotic thera ... | 1999 | 10203517 |
| distribution of chitinase in guinea pig tissues and increases in levels of this enzyme after systemic infection with aspergillus fumigatus. | intravenous infection of guinea pigs with the fungus aspergillus fumigatus resulted in increased levels of chitinase in serum and tissues of the animals. the molecular properties of the enzyme were demonstrated to be different from those of the fungal chitinase, but also from guinea pig lysozyme and beta-n-acetylhexosaminidase. bio-gel p-100 gel filtration showed that in liver, spleen, heart and lung tissue of control animals there were two molecular mass forms present with apparent molecular ma ... | 1999 | 10206706 |
| molecular dissection of mitogillin reveals that the fungal ribotoxins are a family of natural genetically engineered ribonucleases. | mitogillin and the related fungal ribotoxins are highly specific ribonucleases which inactivate the ribosome enzymatically by cleaving the 23-28 s rna of the large ribosomal subunit at a single phosphodiester bond. the site of cleavage occurs between g4325 and a4326 (rat ribosome numbering) which are present in one of the most conserved sequences (the alpha-sarcin loop) among the large subunit ribosomal rnas of all living species. amino acid sequence comparison of ribotoxins and guanyl/purine ri ... | 1999 | 10212236 |
| optically active antifungal azoles. viii. synthesis and antifungal activity of 1-[(1r,2r)-2-(2,4-difluoro- and 2-fluorophenyl)-2-hydroxy-1-methyl-3-(1h-1,2,4-triazol-1-yl)propyl]- 3-(4-substituted phenyl)-2(1h,3h)-imidazolones and 2-imidazolidinones. | new optically active antifungal azoles, 1-[(1r,2r)-2-(2,4-difluoro- and 2-fluorophenyl)-2-hydroxy-1-methyl-3-(1h-1,2,4-triazol-1-yl)propyl ]-3-(4- substituted phenyl)-2(1h,3h)-imidazolones (1,2) and 2-imidazolidinones (3,4), were prepared in a stereocontrolled manner from (1s)-1-[(2r)-2-(2,4- difluoro- and 2-fluorophenyl)-2-oxiranyl]ethanols (15, 16). compounds 1-4 showed potent antifungal activity against candida albicans in vitro and in vivo, as well as a broad antifungal spectrum for various ... | 1999 | 10212385 |
| isolated renal aspergillus abscess in an aids patient with a normal cd4+ cell count on highly active antiretroviral therapy. | isolated renal aspergillus abscess is a very rare complication of hiv infection. it usually occurs in patients with severe immune deficiency. the case of a 29-year-old hiv-infected homosexual male, a nonintravenous drug abuser, who developed a right renal aspergillus abscess despite normalization of the cd4+ cell count after highly active antiretroviral treatment is described. when antimicrobial treatment failed (amphotericin b followed by itraconazole), he was cured by right nephrectomy and rem ... | 1999 | 10219579 |
| [pneumonias in patients with malignant hemopathies. their etiology, response to treatment and prognostic factors in 69 patients (88 episodes)]. | to analyze the etiology, treatment response and prognostic factors in 88 episodes of pneumonia in patients with hematological malignancies diagnosed in one center during a period of 30 months. | 1999 | 10220762 |
| renal aspergillosis giving rise to obstructive uropathy and recurrent anuric renal failure. | a sixty-year-old previously healthy male patient presented with anuric renal failure of sudden onset. he was detected to have aspergillus fumigatus fungal balls in the renal pelvis, ureters and bladder which were removed and his renal function improved. he was treated with itraconazole and sent home. three weeks later he again presented with anuria and renal failure. he had recurrence of the obstruction with the same fungus. the fungal ball was removed, a double 'j' stenting was performed and he ... | 1998 | 10221171 |
| humoral and cell-mediated autoimmune reactions to human acidic ribosomal p2 protein in individuals sensitized to aspergillus fumigatus p2 protein. | a panel of cdnas encoding allergenic proteins was isolated from an aspergillus fumigatus cdna library displayed on the surface of filamentous phage. solid phase-immobilized serum immunoglobulin e (ige) from a. fumigatus-allergic individuals was used to enrich phage displaying ige-binding molecules. one of the cdnas encoded a 11.1-kd protein that was identified as acidic ribosomal phosphoprotein type 2 (p2 protein). the allergen, formally termed rasp f 8, shares >62% sequence identity and >84% se ... | 1999 | 10224291 |
| ige-mediated reactions to autoantigens in allergic diseases. | there is increasing evidence that ige-mediated reactivity against autoantigens could play a role in the pathology of severe chronic atopic diseases. | 1999 | 10224375 |
| resistance of aspergillus fumigatus to itraconazole. | | 1998 | 10225408 |
| production, characterization, and epitope mapping of a monoclonal antibody against aspartic proteinase of candida albicans. | a monoclonal antibody (mab; mab cap1) that was reactive with extracellular aspartic proteinase of candida albicans (cap) was produced. the mab showed strong sensitivity and reactivity to cap but not to the aspartic proteinases of candida parapsilosis, candida tropicalis, and aspergillus fumigatus or to human cathepsin d or porcine pepsin. the epitope of the cap recognized by the mab was the proteinaseous part of cap and the putative epitope of the mab was located in the asp77 to gly103 sequence. ... | 1999 | 10225850 |
| conformational and linear b-cell epitopes of asp f 2, a major allergen of aspergillus fumigatus, bind differently to immunoglobulin e antibody in the sera of allergic bronchopulmonary aspergillosis patients. | asp f 2 is a major aspergillus fumigatus allergen involved in allergic bronchopulmonary aspergillosis. knowledge of the b-cell epitopes may contribute to the understanding of immunoregulation and immunodiagnosis. to elucidate the immunoglobulin e (ige) binding epitopes in the linear sequence of asp f 2, we synthesized decamer peptides spanning the whole molecule of asp f 2 on derivatized cellulose membranes and evaluated ige binding in abpa patient and control sera. peptides three to five amino ... | 1999 | 10225885 |
| zinc-regulated biosynthesis of immunodominant antigens from aspergillus spp. | aspnd1 and aspf2 are immunodominant antigens from aspergillus nidulans and a. fumigatus, respectively, that are readily synthesized in infections in the human host, as demonstrated by their reactivity with more than 80% of sera from patients with aspergilloma or allergic bronchopulmonary aspergillosis. we demonstrate here that both antigens are exclusively produced under situations of low bioavailability of free zn2+. addition of micromolar concentrations of zn2+ to the culture medium strongly s ... | 1999 | 10225898 |
| characterization of allergens from penicillium oxalicum and p. notatum by immunoblotting and n-terminal amino acid sequence analysis. | penicillium species are important causative agents of extrinsic bronchial asthma. however, little is known about the allergens of these ubiquitous fungal species. objective the object was to analyse the composition, the allergenic cross-reactivity and the n-terminal sequences of allergens from two prevalent airborne penicillium species, p. oxalicum and p. notatum. | 1999 | 10231324 |
| in vitro antifungal susceptibilities of candida albicans and other fungal pathogens to polygodial, a sesquiterpene dialdehyde. | in vitro antifungal activities of polygodial were investigated against several fungal pathogens. polygodial showed strong antifungal activity, comparable to amphotericin b, against yeast-like fungi candida albicans, c. utilis, c. krusei, cryptococcus neoformans, saccharomyces cerevisiae and also filamentous fungi including trichophyton mentagrophytes, t. ruburum, and penicillium marneffei. other strains such as aspergillus fumigatus, a. flavus, p. chrysogenum, c. lipolytica, and c. tropicalis sh ... | 1999 | 10232062 |
| [the role of virulence factor of elastolytic proteinase from aspergillus fumigatus]. | the role of virulence factor of elastolytic proteinase produced by aspergillus fumigatus was investigated in mice immunocompromised with cyclophosphamide (cy) to block neutrophil and lymphocyte functions or with carrageenan (ca) to block the phagocytic activity of macrophages. these mice were infected through inhalation with the spores of elastolytic proteinase producing or nonproducing strains of a. fumigatus, and then were observed daily for 15 days or until death. compared to cy-immunocomprom ... | 1999 | 10234079 |
| aspergillus osteomyelitis in a child who has p67-phox-deficient chronic granulomatous disease. | here we describe aspergillus osteomyelitis of the tibia in a 9-year-old boy who has an autosomal recessive form of chronic granulomatous disease (cgd). the patient showed a p67-phagocyte oxidase (phox) deficiency, which is rare type of cgd in japan. the initial treatment which consisted of surgical debridement and antibiotic therapy with amphotericin b (amph), did not control the infection. aspergillus fumigatus (a. fumigatus) pure isolated from drainage fluid and necrotic bone tissue demonstrat ... | 1999 | 10319618 |
| prostatic and renal aspergillosis in a patient with aids. | | 1999 | 10320130 |
| mycotic keratitis in non-steroid exposed vernal keratoconjunctivitis. | to report a patient with vernal keratoconjunctivitis who developed mycotic keratitis in absence of known risk factors. | 1999 | 10321546 |
| density and molecular epidemiology of aspergillus in air and relationship to outbreaks of aspergillus infection. | after five patients were diagnosed with nosocomial invasive aspergillosis caused by aspergillus fumigatus and a. flavus, a 14-month surveillance program for pathogenic and nonpathogenic fungal conidia in the air within and outside the university hospital in rotterdam (the netherlands) was begun. a. fumigatus isolates obtained from the department of hematology were studied for genetic relatedness by randomly amplified polymorphic dna (rapd) analysis. this was repeated with a. fumigatus isolates c ... | 1999 | 10325319 |
| [renal and perirenal abscess]. | renal and perirenal abscesses are rare infections of the urinary tract traditionally caused by staphylococcus aureus. today however there is a predominance of abscesses secondary to coliform bacteria such as e. coli. this paper presents a revision of our series over the last ten years (1987-1996). a total of 11 abscesses (3 renal and 8 perinephritic) were recorded. the most frequent symptom for visiting the clinic was back pain. all patients had predisposing associated conditions. the microbiolo ... | 1999 | 10327677 |
| occlusion of the internal carotid artery by means of microcoils for preventing epistaxis caused by guttural pouch mycosis in horses. | occlusion of the internal carotid artery by insertion of intravascular platinum microcoils for guttural pouch mycosis was experimentally evaluated in 9 healthy adult thoroughbred horses. the internal carotid artery was ligated to its origin, and an arteriotomy was made distal to the ligature, which was then occluded by insertion of the microcoil approximately 13 cm distal to its origin. cessation of blood flow was determined visually and by angiography at the arteriotomy site. six horses were ev ... | 1999 | 10331192 |
| the neuropathology of intestinal failure and small bowel transplantation. | the aim of this study was to elucidate the neuropathological substrate of intestinal failure before and after small bowel transplantation (sbt). retrospective analysis of complete autopsy or brain biopsy specimens of 17 patients with intestinal failure (12 children and 5 adults) were studied. patients were divided into two groups. group i (transplanted group; n = 13) included those patients who underwent intestinal transplantation under tacrolimus and steroids immunosuppressive therapy. group ii ... | 1999 | 10334488 |
| effects of methylprednisolone and cyclosporine a on fungal growth in vitro. | the management of corneal transplants after mycotic keratitis often poses a therapeutic dilemma. clinicians are hesitant to use topical steroids because of their potential enhancement of fungal growth. this study seeks to evaluate the in vitro effects of methylprednisolone and cyclosporine a on the growth of various molds that often are responsible for keratomycoses. | 1999 | 10336034 |
| interleukin-12 enhances antifungal activity of human mononuclear phagocytes against aspergillus fumigatus: implications for a gamma interferon-independent pathway. | the potential of recombinant human interleukin-12 (il-12) to enhance the capacity of human monocytes (mnc) to elicit an oxidative burst and damage hyphae of aspergillus fumigatus was investigated. incubation of peripheral blood mononuclear cells (pbmc) from healthy adults with 10 to 100 ng of il-12/ml at 37 degrees c for 2 to 3 days enhanced the production of superoxide anion (o2-) in response to phorbol myristate acetate (pma) (p = 0.04) and unopsonized a. fumigatus hyphae (p = 0.03) and furthe ... | 1999 | 10338518 |
| aspergillus fumigatus endophthalmitis in a patient with acute myeloid leukaemia. | a 55-year-old patient developed progressive loss of vision in one eye following induction chemotherapy for acute myeloid leukaemia (aml). aspergillus fumigatus was cultured from vitreal aspirates. the patient was treated with intravenous and intravitreal amphotericin b but suffered complete loss of vision in her right eye. we believe this is the first report of culture-proven aspergillus fumigatus endophthalmitis in a patient treated for a haematological malignancy. | 1999 | 10342076 |
| specific igg subclass antibody pattern to aspergillus fumigatus in patients with cystic fibrosis with allergic bronchopulmonary aspergillosis (abpa). | igg and igg subclass antibodies to aspergillus fumigatus (a fumigatus) were measured in a large population of patients with cystic fibrosis to elucidate a putative antibody pattern specific for allergic bronchopulmonary aspergillosis (abpa). | 1999 | 10343631 |
| antifungal jujubogenin saponins from colubrina retusa. | antifungal assay-guided isolation of the 95% ethanol extract of the stems of colubrina retusa yielded jujubogenin 3-o-alpha-l-arabinofuranosyl(1-->2)-[beta-d-glucopyranosyl (1-->3)]-alpha-l-arabinopyranoside (1), which showed modest growth-inhibitory effects against candida albicans, cryptococcus neoformans, and aspergillus fumigatus (mics, 50 microg/ml). in addition, two new minor saponins, jujubogenin 3-o-alpha-l-arabinofuranosyl(1-->2)-[2-o-(trans, cis)p-coumaroyl-beta-d-glucopyranosyl(1-->3) ... | 1999 | 10346942 |
| in vitro antifungal activity of nikkomycin z in combination with fluconazole or itraconazole. | nikkomycins are nucleoside-peptide antibiotics produced by streptomyces species with antifungal activities through the inhibition of chitin synthesis. we investigated the antifungal activities of nikkomycin z alone and in combination with fluconazole and itraconazole. checkerboard synergy studies were carried out by a macrobroth dilution procedure with rpmi 1640 medium at ph 6.0. at least 10 strains of the following fungi were tested: candida albicans, other candida spp., cryptococcus neoformans ... | 1999 | 10348760 |
| the influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage. | the aerobic decomposition of barley silage treated with two inoculants (laca and lacb) containing mixtures of lactobacillus plantarum and enterococcus faecium was investigated over a 28-day period. initially, yeast and bacterial populations were larger in silage inoculated with laca than in silage treated with lacb or water alone (control). differences in the succession of yeasts in silage treated with laca were observed relative to the other two treatments. from silage treatment with laca, issa ... | 1999 | 10349723 |
| collaboration of human phagocytes with ly 303366 for antifungal activity against aspergillus fumigatus. | ly 303366, an inhibitor of 1, 3-beta-d-glucan synthase, was tested alone, or in co-culture with neutrophils or monocytes, for antifungal activity against aspergillus fumigatus using the xtt metabolism assay. ly 303366 at 0.1 mg/l for 48 h significantly inhibited growth by conidia in a microtest plate xtt assay system. inhibition was similar if the drug was removed after only 24 h. microscopically this correlated with less growth and stunted malformed hyphae. ly 303366 (0.1 mg/l) also inhibited t ... | 1999 | 10350377 |
| hilar adenopathy in allergic bronchopulmonary aspergillosis. | a 20-year-old male student developed allergic bronchopulmonary aspergillosis (abpa). computed tomography (ct) of the thorax done to detect central bronchiectasis (cb) for confirmation of diagnosis revealed, in addition, right hilar lymphadenopathy. hilar adenopathy is thought to be rare in abpa and has been documented only once before. because of the finding of hilar adenopathy, the earlier reported patient had to undergo an invasive surgical procedure. | 1999 | 10353584 |
| characterization of sphingolipids from mycopathogens: factors correlating with expression of 2-hydroxy fatty acyl (e)-delta 3-unsaturation in cerebrosides of paracoccidioides brasiliensis and aspergillus fumigatus. | significant differences exist between mammals and fungi with respect to glycosphingolipid (gsl) structure and biosynthesis. thus, these compounds, as well as the cellular machinery regulating their expression, have considerable potential as targets for the diagnosis and treatment of fungal diseases. in this study, the major neutral gsl components extracted from both yeast and mycelium forms of the thermally dimorphic mycopathogen paracoccidioides brasiliensis were purified and characterized by 1 ... | 1999 | 10353841 |
| multiple renal aspergillus abscesses in an aids patient: contrast-enhanced helical ct and mri findings. | renal insufficiency or allergic reactions for x-ray contrast agents are frequent limitations in immunocompromised hosts such as neutropenic or aids patients. due to a better tolerance of contrast agents in mri, this technique is well suited for investigation of parenchymal organs. we demonstrate an allergic aids patient who presented with fever and flank pain. at sonography, anechoic renal lesions were supposed to be non-complicated cysts; however, on t2-weighted mri, the center was of high sign ... | 1999 | 10354871 |
| sino-orbital aspergillosis in a dog. | fungal rhinitis-sinusitis with orbital involvement was diagnosed in a dog with profuse unilateral ocular and bilateral nasal discharge, enophthalmos, and a corneal descemetocele. the descemetocele was treated with a conjunctival pedicle graft. frontal sinusotomy was performed, and clotrimazole was infused through catheters placed in the frontal sinuses and nasal cavities to saturate the tissues for 1 hour. successful resolution of orbital infection may have been aided by a fistula between the fr ... | 1999 | 10363096 |
| in vitro amphotericin b resistance in clinical isolates of aspergillus terreus, with a head-to-head comparison to voriconazole. | amphotericin b therapy continues to be the "gold standard" in the treatment of invasive aspergillosis in the immunocompromised host. although aspergillus fumigatus and aspergillus flavus constitute the major species, several reports have described invasive pulmonary or disseminated disease due to the less common aspergillus terreus and dismal clinical outcomes with high-dose amphotericin b. we therefore evaluated 101 clinical isolates of a. terreus for their susceptibility to amphotericin b and ... | 1999 | 10364610 |
| binding of asp-hemolysin from aspergillus fumigatus to oxidized low density lipoprotein. | asp-hemolysin is a human low density lipoprotein (ldl) binding protein. we found evidence that asp-hemolysin binds to oxidized ldl (oxldl) as well as to ldl in a concentration-dependent manner. this result suggests that asp-hemolysin is a novel protein, which shares binding abilities to ldl and oxldl. | 1999 | 10375182 |
| fundamental signals that regulate eosinophil homing to the gastrointestinal tract. | the histological identification of increased eosinophils in the gastrointestinal tract occurs in numerous clinical disorders; however, there is a limited understanding of the mechanisms regulating eosinophil trafficking into this mucosal surface. the results presented in this study characterize the processes regulating eosinophil homing into the gastrointestinal tract at baseline. eosinophils were found to be present in the lamina propria of 19-day-old embryos and germ-free adult mice at concent ... | 1999 | 10377178 |
| [does allergic fungal sinusitis exist? preliminary results of a prospective study]. | allergic aspergillar sinusitis is a very controversial clinical feature. we present results of a prospective study aimed at evaluating the reality of allergic aspergillar sinusitis in a nosologic and clinical point of vue. during a 5 months period, 31 patients underwent surgery: 21 sino-nasal polyposis, 5 chronic sinusitis without polyposis, 5 chronic sinusitis with radiologic images evocative of mycosis. the study was carried out using clinical criteria (per-operative discovery of glue-like, th ... | 1999 | 10378036 |
| immune responses to aspergillus antigen in il-4-/-mice and the effect of eosinophil ablation. | exposure to aspergillus fumigatus allergens results in enhanced total serum ige and peripheral blood eosinophils in mice. the associated pulmonary inflammation and immunologic responses are comparable to those detected in human allergic bronchopulmonary aspergillosis. allergen-induced cytokines are thought to regulate the inflammatory and immune responses in these animals. | 1999 | 10380772 |
| [successful treatment of allergic bronchopulmonary aspergillosis with erythromycin and fluconazole]. | a 30-year-old woman was admitted to our hospital because of productive cough, wheezing, and the disclosure of abnormal shadows on chest x-ray films. the patient was given a diagnosis of allergic bronchopulmonary aspergillosis (abpa) based on eight findings: asthma, eosinophilia, elevated serum ige concentrations, immediate skin reactivity to aspergillus antigen, the presence of precipitating antibodies against aspergillus antigen, lung infiltration, central bronchiectasis, and repeated culture o ... | 1999 | 10390974 |
| systemic mycoses during prophylactical use of liposomal amphotericin b (ambisome) after liver transplantation. | we investigated the prophylactical administration of liposomal amphotericin b (ambisome) in the early phase after liver transplantation (ltx). fifty-eight patients received ambisome prophylactically after ltx. ambisome (1 mg kg-1 day-1) was given intravenously for 7 days after ltx. immunosuppressive prophylaxis was cyclosporin a (csa) based in 11 patients. forty-seven patients had a tacrolimus-based immunosuppressive regimen. csa and tacrolimus dosages were adjusted to trough levels of 150-250 n ... | 1999 | 10394848 |
| synthetic analogues of irlbacholine: a novel antifungal plant metabolite isolated from irlbachia alata. | irlbacholine and a series of related analogues were synthesized and their antifungal activities against candida albicans, cryptococcus neoformans, and aspergillus fumigatus were assessed. the natural bisphosphocholine, irlbacholine, was the most potent compound, its 22-carbon chain length appearing to be optimal. | 1999 | 10395496 |
| in vitro allergen-induced degranulation of pulmonary mast cells from horses with recurrent airway obstruction (heaves). | to determine the capacity of pulmonary mast cells (pmc) to degranulate in response to various potential allergens and other secretagogues in horses with recurrent airway obstruction (heaves) and clinically normal horses before and after exposure to moldy hay. | 1999 | 10407477 |
| immunoblotting technique for the detection of allergens of aspergillus fumigatus: influence of nonidet p-40 on the sensitivity. | immunoblotting provides a useful technique for the study of antigens, antibodies and allergens. to overcome problems regarding the loss of antigenic properties during the blotting and developing procedures, several solutions have been described. the inclusion of nonidet p-40, recommended to increase the sensitivity of developing procedures for immunoblots, in an existing procedure for the detection of allergens of aspergillus fumigatus, however, led to decreased sensitivity of the method. | 1999 | 10412684 |
| comparative investigations of airborne culturable microorganisms in selected waste treatment facilities and in neighbouring residential areas. | the evaluation of airborne microorganisms in waste treatment facilities is complicated by different measuring systems, a lack of measuring standards and large variations between individual counts. in the present study, different sectors of the waste management industry were compared by determining median values of airborne culturable microorganisms from numerous parallel counts over a prolonged time period. the samples were taken during the warm season using the six-stage andersen volumetric sam ... | 1999 | 10418096 |
| production of nitric oxide by rat alveolar macrophages stimulated by cryptococcus neoformans or aspergillus fumigatus. | cryptococcus neoformans and aspergillus fumigatus are airborne fungi and the alveolar macrophages (am) constitute a first line of host defence against both pathogens. we investigated the ability of rat am to produce nitric oxide (no) when challenged in vitro with c. neoformans, a. fumigatus conidia or inert silica particles alone and together with interferon gamma (ifn-gamma). the role of no in the killing of c. neoformans as well as the relationship between phagocytosis of the yeast or a. fumig ... | 1999 | 10421846 |
| cytokine and chemokine responses following pulmonary challenge with aspergillus fumigatus: obligatory role of tnf-alpha and gm-csf in neutrophil recruitment. | aspergillus fumigatus causes life-threatening invasive pulmonary aspergillosis in the immunocompromised patient. in this study we have used a murine model of intratracheal challenge with a. fumigatus to investigate the recruitment of inflammatory cells in the lung and the expression of proinflammatory cytokines and chemokines. our results show that a. fumigatus causes an acute pulmonary inflammatory response which is dominated by neutrophils and to a lesser extent macrophages. during the peak of ... | 1999 | 10421850 |
| determination of optimum growth conditions for gliotoxin production by aspergillus fumigatus and development of a novel method for gliotoxin detection. | gliotoxin is a toxic metabolite of aspergillus fumigatus fresenius and other fungi. it has been suggested that this toxin may play an important role in the pathogenesis of aspergillosis as gliotoxin has immunosuppressive activity both in vitro and in vivo. we have determined the optimum growth conditions for the production of gliotoxin by selected isolates of a. fumigatus using a number of defined media. gliotoxin was detected by thin layer chromatography and high performance liquid chromatograp ... | 1999 | 10421857 |
| aerobiological analysis in a salami factory: a possible case of extrinsic allergic alveolitis by penicillium camembertii. | a 39-year-old man was hospitalized with a history of fatigue, dyspnoea and low grade fever which seemed to be related to his working environment. the patient was employed in a salami factory, working near the area where the salami are seasoned with fungal inocula. chest x-ray showed diffuse initial changes of reticulonodular pattern that disappeared after a brief course of steroids therapy. precipitating antibodies to penicillium notatum and aspergillus fumigatus were found both in plasma and br ... | 1999 | 10421864 |
| aurantosides d, e, and f: new antifungal tetramic acid glycosides from the marine sponge siliquariaspongia japonica. | aurantosides d-f (4-6), new polyene tetramic acids comprising an n-trisaccharide unit, have been isolated from the marine sponge siliquariaspongia japonica. their structures were determined by spectral and chemical methods. a reinvestigation of nmr data of the previously isolated aurantosides a and b led to revision of the geometry of the terminal double bond. aurantosides exhibit potent antifungal activity against aspergillus fumigatus and candida albicans. | 1999 | 10425118 |