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iron requirement and iron uptake from various iron compounds by different plant species.the fe requirements of four monocotyledonous plant species (avena sativa l., triticum aestivum l., oryza sativa l., zea mays l.) and of three dicotyledonous species (lycopersicum esculentum mill., cucumis sativus l., glycine maxima (l.) merr.) in hydroponic cultures were ascertained. fe was given as nafe-eddha chelate (fe ethylenediamine di (o-hydroxyphenylacetate). i found that the monocotyledonous species required a substantially higher fe concentration in the nutrient solution in order to att ...197416658933
subcellular localization of the red-absorbing form of phytochrome by immunocytochemistry.an immunocytochemical technique was used to localize the red-absorbing form of phytochrome at the light- or electron-microscope level in etiolated barley (hordeum vulgare l.) coleoptile tip, rice (oryza sativa l.) coleoptilar node, maize (zea mays l.) coleoptile tip, rye (secale cereale l.) coleoptile tip and coleoptilar node, and oat (avena sativa l.) root cap. staining for phytochrome in the cells was found to be generally distributed throughout the cytoplasm. in addition, barley also showed s ...197424442776
the effect of green synthesized cuo nanoparticles on callogenesis and regeneration of oryza sativa l.in this study, we have investigated the effect of copper oxide nanoparticles (cuo-nps) on callogenesis and regeneration of oryza sativa l (super basmati, basmati 2000, basmati 370, and basmati 385). in this regard, cuo-nps have been bio-synthesized via azadirachta indica leaf extract. scanning electron microscope (sem) analysis depicts average particle size of 40 ± 5 nm with highly homogenous and spherical morphology. x-ray diffraction (xrd) and fourier transform infrared spectroscopy (ftir) hav ...201627630655
increase in growth, productivity and nutritional status of wheat (triticum aestivum l. cv. wh-711) and enrichment in soil fertility applied with organic matrix entrapped urea.field experiments were conducted during two consequent years in semi-arid, subtropical climate of rohtak district situated in north-west indian state haryana to evaluate the effects of eco-friendly organic matrix entrapped urea (omeu) on wheat (triticum aestivum l. cv. wh-711). the omeu prepared in granular form contained cow dung, rice bran (grain cover of oryza sativa), neem (azadirachta indica) leaves and clay soil (diameter of particles < 0.002 mm) in 1:1:1:1 ratios and saresh (plant gum of ...201324006800
ethnoveterinary remedies of diseases among milk yielding animals in kathua, jammu and kashmir, india.the triangle of relationship between human beings, animals and plants has existed for ages, and has given rise to intense-relationships and consequently rich traditions of ethnoveterinary knowledge throughout the world. the predominantly rural population and the strong agricultural base have provided unique situation for rich ethnoveterinary practices in the study area.201222366093
anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longetivity.the exposure to ultraviolet radiations (uvr) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ros), leading to aging. the skin can be treated and protected from the injurious effects of ros by using various pharmaceutical formulations, such as cream. cream can be loaded with antioxidants to quench ros leading to photo-protective effects. moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. this review arti ...201526448818
review of anthraquinone applications for pest management and agricultural crop protection.we have reviewed published anthraquinone applications for international pest management and agricultural crop protection from 1943 to 2016. anthraquinone (aq) is commonly found in dyes, pigments and many plants and organisms. avian repellent research with aq began in the 1940s. in the context of pest management, aq is currently used as a chemical repellent, perch deterrent, insecticide and feeding deterrent in many wild birds, and in some mammals, insects and fishes. criteria for evaluation of e ...201627252044
growth and cadmium phytoextraction by swiss chard, maize, rice, noccaea caerulescens, and alyssum murale in ph adjusted biosolids amended soils.past applications of biosolids to soils at some locations added higher cd levels than presently permitted. cadmium phytoextraction would alleviate current land use constraints. unamended farm soil, and biosolids amended farm and mine soils were obtained from a fulton co., il biosolids management facility. soils contained 0.16, 22.8, 45.3 mg cd kg(-1) and 43.1, 482, 812 mg zn kg(-1) respectively with initial ph 6.0, 6.1, 6.4. in greenhouse studies, swiss chard (beta vulgaris var. cicla), a cd-acc ...201525174422
mitochondrial genome sequence of the legume vicia faba.the number of plant mitochondrial genomes sequenced exceeds two dozen. however, for a detailed comparative study of different phylogenetic branches more plant mitochondrial genomes should be sequenced. this article presents sequencing data and comparative analysis of mitochondrial dna (mtdna) of the legume vicia faba. the size of the v. faba circular mitochondrial master chromosome of cultivar broad windsor was estimated as 588,000 bp with a genome complexity of 387,745 bp and 52 conservative mi ...201323675376
the complete sequence of the rice (oryza sativa l.) mitochondrial genome: frequent dna sequence acquisition and loss during the evolution of flowering plants.the entire mitochondrial genome of rice (oryza sativa l.), a monocot plant, has been sequenced. it was found to comprise 490,520 bp, with an average g+c content of 43.8%. three rrna genes, 17 trna genes and five pseudo trna sequences were identified. in addition, eleven ribosomal protein genes and two pseudo ribosomal protein genes were found, which are homologous to 13 of the 16 genes for ribosomal proteins in the mitochondrial genome of the liverwort (marchantia polymorpha). a greater degree o ...200212471441
localization of glyoxylate-cycle marker enzymes in peroxisomes of senescent leaves and green cotyledons.crude particulate homogenates from leaves of barley (hordeum vulgare l.), rice (oryza sativa l.), leaf-beet (beta vulgaris var. cicla l.) and pumpkin (cucurbita pepo l.) cotyledons were separated on sucrose density gradients. the peroxisomal fractions appeared at a buoyant density of 1.25 g·cm(-3) and contained most of the activities of catalase (ec 1.11.1.6), and hydroxypyruvate reductase (ec 1.1.1.81) on the gradients. in peroxisomal fractions from detached leaves and green cotyledons incubate ...199024202025
uptake and decomposition of the herbicide propanil in the plant bidens pilosa l. dominating in the yangtze three gorges reservoir (tgr), china.propanil (3',4'-dichloropropionanilide) is a selective post emergence herbicide for controlling broad leaf and grass weeds in rice (oryza sativa l.). after being taken up by plants, the fate of propanil in decomposing plant material is of particular importance to the phytoremediation of the environment. therefore, we investigated the biotransformation of propanil in the plant bidens pilosa under conditions close to those present in the three gorges reservoir (tgr), china. plants pre-treated with ...201626846318
genomic selection and association mapping in rice (oryza sativa): effect of trait genetic architecture, training population composition, marker number and statistical model on accuracy of rice genomic selection in elite, tropical rice breeding lines.genomic selection (gs) is a new breeding method in which genome-wide markers are used to predict the breeding value of individuals in a breeding population. gs has been shown to improve breeding efficiency in dairy cattle and several crop plant species, and here we evaluate for the first time its efficacy for breeding inbred lines of rice. we performed a genome-wide association study (gwas) in conjunction with five-fold gs cross-validation on a population of 363 elite breeding lines from the int ...201525689273
effect of substituting brown rice for corn on lactation and digestion in dairy cows fed diets with a high proportion of grain.the effects of the substitution of brown rice (oryza sativa l.; br) for corn (zea mays l.) in ensiled total mixed ration (tmr) that had a high proportion of grain on feed intake, lactation performance, ruminal fermentation, digestion, and n utilization were evaluated. nine multiparous holstein cows (51 ± 9 d in milk) were used in a replicated 3 × 3 latin square design with 3 dietary treatments: a diet containing 0, 20, or 40% steam-flaked br and 40, 20, or 0% steam-flaked corn (dry matter basis) ...201424342688
sustainable reuse of rice residues as feedstocks in vermicomposting for organic fertilizer production.over the past decade, rice (oryza sativa or oryza glaberrima) cultivation has increased in many rice-growing countries due to the increasing export demand and population growth and led to a copious amount of rice residues, consisting mainly of rice straw (rs) and rice husk (rh), being generated during and after harvesting. in this study, eudrilus eugeniae was used to decompose rice residues alone and rice residues amended with cow dung (cd) for bio-transformation of wastes into organic fertilize ...201423900949
investigation of the differences in thermal stability of two recombinant human serum albumins with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine liposomes by uv circular dichroism spectropolarimetry.previous studies have demonstrated that liposome-protein interactions can result in changes to the thermal stability of the protein. we utilized far-uv circular dichroism spectropolarimetry and fluorescence spectroscopy to investigate the interaction of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (dppc) liposomes with two recombinant human serum albumins (rhsa). we demonstrate that rhsa expressed in oryza sativa (osrhsa) has improved secondary structure thermal stability compared to rhsa express ...201222423959
ch₄ and n₂o emissions from different varieties of forage rice (oryza sativa l.) treating liquid cattle waste.to evaluate global warming potential (gwp) on livestock waste treatment and biomass production in rice field, methane (ch(4)) and nitrous oxide (n(2)o) fluxes from forage rice fields planted with 4 different cultivars (oryza sativa l. cv. hamasari, leafstar, kusahonami and takanari) were measured. each of the cultivars were subjected either to basal fertilization alone (control plots) (84 kg n ha(-1)), or to basal fertilization plus topdressing with liquid cattle waste or lcw (treatment plots) ( ...201222289172
simple and efficient way to detect small polymorphic bands in plants.there are many ways to detect polymorphism. in this study we use the microsatellite markers to detect the polymorphism for the salt tolerance. this method has been successfully conducted in oryza sativa and brassica juncea. the results are reproducible. in contrast to previous methods, our method is simple and quite accurate for detecting the polymorphic bands. in this study instead of using agarose gel and ethidium bromide staining, we used non-denaturing polyacrylamide gel and a low-cost impro ...201526484259
application of a rotation system to oilseed rape and rice fields in cd-contaminated agricultural land to ensure food safety.this field experiment analyzed the phytoremediation effects of oilseed rape in moderately cadmium (cd)-contaminated farmland and the food safety of successive rice in an oilseed rape-rice rotation system. two oilseed rape cultivars accumulated cd at different rates. the rapeseed cultivar zhucang huazi exhibited high cd accumulation rates, higher than the legal limit for human consumption (0.2mgkg(-1)); cd concentrations in the cultivar chuanyou ii-93 were all below the maximum allowed level. pla ...201425108175
oryza sativa polyamine oxidase 1 back-converts tetraamines, spermine and thermospermine, to spermidine.oryza sativa polyamine oxidase 1 back-converts spermine (or thermospermine) to spermidine. considering the previous work, major path of polyamine catabolism in rice plant is suggestive to be back-conversion but not terminal catabolism. rice (oryza sativa) contains seven genes encoding polyamine oxidases (paos), termed ospao1 to ospao7, based on their chromosomal number and gene id number. we previously showed that three of these members, ospao3, ospao4 and ospao5, are abundantly expressed, that ...201424105034
mitochondrial atpa gene is altered in a new orf220-type cytoplasmic male-sterile line of stem mustard (brassica juncea).the purpose of this research is to identify the probable mitochondrial factor associated with cytoplasmic male sterility (cms) by comparative analysis of cms and its isogenic maintainer lines in stem mustards. dramatic variations in the morphology of floral organs were observed in cms stem mustard. mitochondrial atpa gene was shown to be altered in cms compared with that in its maintainer line, of which mitochondrial atpa gene from its maintainer line was sequenced to encode 507 amino acids. it ...200918026850
proline suppresses rubisco activity in higher plants.seedlings of brassica juncea, sesbania sesban, and oryza sativa exposed to salt stress accumulated proline to levels as high as 4- to 20-fold over those of controls. because chloroplasts are the major site for synthesis of stress induced proline accumulation, in vitro studies were conducted to see how the over-accumulation of this solute influences the activity of the major chloroplastic enzyme ribulose 1,5-bis-phosphate carboxylase (rubisco) purified from the above plant species. surprisingly, ...19989826546
nitrogen fertilizer management for enhancing crop productivity and nitrogen use efficiency in a rice-oilseed rape rotation system in china.the use of efficient rates of nitrogen (n) fertilizer application is important with regard to increasing crop productivity and maintaining environmental sustainability. rice-oilseed rape rotations are a mainstay of the economy and food security of china. therefore, a field experiment was carried out during 2011-2013 in honghu to identify the most appropriate n application rates for enhancing crop productivity and n use efficiency for rice (oryza sativa l.)-oilseed rape (brassica napus l.) rotati ...201627746809
enhanced salt tolerance under nitrate nutrition is associated with apoplast na+ content in canola (brassica. napus l.) and rice (oryza sativa l.) plants.to analyze the effect of nitrogen form on salt stress, we studied the response of two different plant species, canola (brassica napus l.), a dicotyledon which prefers no3(-) nutrition, and rice (oryza sativa l.), a monocotyledon which prefers nh4(+) nutrition, to salt stress under no3(-) (nn) and nh4(+) (an) nutrition. salt stress was simulated by the addition of 150 and 100 mm nacl to nn (nns) and an (ans) in canola and rice seedlings, respectively. salt stress induced reductions of shoot and r ...201627519313
modern breeding and biotechnological approaches to enhance carotenoid accumulation in seeds.there is an increasing demand for carotenoids, which are fundamental components of the human diet, for example as precursors of vitamin a. carotenoids are also potent antioxidants and their health benefits are becoming increasingly evident. protective effects against prostate cancer and age-related macular degeneration have been proposed for lycopene and lutein/zeaxanthin, respectively. additionally, β-carotene, astaxanthin and canthaxanthin are high-value carotenoids used by the food industry a ...201627485229
characteristics of heavy metal transfer and their influencing factors in different soil-crop systems of the industrialization region, china.soil heavy metals and their bioaccumulation in agricultural products have attracted widespread concerns, yet the transfer and accumulation characteristics of heavy metals in different soil-crop systems was rarely investigated. soil and crop samples were collected from the typical agricultural areas in the yangtze river delta region, china. the concentrations of cu, pb, zn, cd and hg in the soils, roots and grains of rice (oryza sativa l.), wheat (triticum l.) and canola (brassica napus l.) were ...201626771531
analysis of aliphatic waxes associated with root periderm or exodermis from eleven plant species.aliphatic waxes can be found in association with suberized tissues, including roots. non-polar lipids were isolated by rapid solvent extraction of mature regions of intact roots from eleven angiosperms, including both monocots and dicots. the majority of roots analyzed were taproots or tuberous taproots that had undergone secondary growth and thus were covered by a suberized periderm. the exceptions therein were maize (zea mays l.) and rice (oryza sativa l.), which present a suberized exodermis. ...201526143051
effects of winter covering crop residue incorporation on ch₄ and n₂o emission from double-cropped paddy fields in southern china.residue management in cropping systems is useful to improve soil quality. however, the studies on the effects of residue management on methane (ch4) and nitrous oxide (n2o) emission from paddy field in southern china are few. therefore, the emissions of ch4 and n2o were investigated in double cropping rice (oryza sativa l.) systems with different winter covering crops using the static chamber-gas chromatography technique to assess the effects of different covering crops on the emissions of green ...201525913315
a simple sample preparation approach based on hydrophilic solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry for determination of endogenous cytokinins.cytokinins (cks), a vital family of phytohormones, play important roles in the regulation of shoot and root development. however, the quantification of cks in plant samples is frequently affected by the complex plant matrix. in the current study, we developed a simple, rapid and efficient hydrophilic interaction chromatography-solid phase extraction (hilic-spe) method for cks purification. cks were extracted by acetonitrile (acn) followed by hilic-spe (silica as sorbents) purification. the extra ...201324212141
accumulation and translocation of 198hg in four crop species.the uptake and transport of mercury (hg) through vegetation play an important role in the biogeochemical cycling of hg. however, quantitative information regarding hg translocation in plants is poorly understood. in the present study, hg uptake, accumulation, and translocation in 4 crops-rice (oryza.sativa l.), wheat (triticum l.), corn (zea mays l.), and oilseed rape (brassica campestris l.)-grown in hoagland solution were investigated using a stable isotope ((198)hg) tracing technique. the dis ...201424173818
a novel membrane-bound e3 ubiquitin ligase enhances the thermal resistance in plants.high temperature stress disturbs cellular homoeostasis and results in a severe retardation in crop growth and development. thus, it is important to reveal the mechanism of plants coping with heat stress. in this study, a novel gene that we identified from brassica napus, referred to as bntr1, was found to play a key role in heat stress response in planta. bntr1 is a membrane-bound ringv (c₄hc₃) protein that displays e3 ligase activity in vitro. we demonstrated that modest expression of bntr1 is ...201424102799
temporal changes in climatic variables and their impact on crop yields in southwestern china.knowledge of variability in climatic variables changes and its impact on crop yields is important for farmers and policy makers, especially in southwestern china where rainfed agriculture is dominant. in the current study, six climatic parameters (mean temperature, rainfall, relative humidity, sunshine hours, temperature difference, and rainy days) and aggregated yields of three main crops (rice: oryza sativa l., oilseed rape: brassica napus l., and tobacco: nicotiana tabacum l.) during 1985-201 ...201423736776
comparative mapping reveals similar linkage of functional genes to qtl of yield-related traits between brassica napus and oryza sativa.oryza sativa and brassica napus-two important crops for food and oil, respectively-share high seed yield as a common breeding goal. as a model plant, o. sativa genomics have been intensively investigated and its agronomic traits have been advanced. in the present study, we used the available information on o. sativa to conduct comparative mapping between o. sativa and b. napus, with the aim of advancing research on seed-yield and yield-related traits in b. napus. firstly, functional markers (fro ...201222942086
effects of rapeseed residue on lead and cadmium availability and uptake by rice plants in heavy metal contaminated paddy soil.rapeseed (brassica napus l.) has been cultivated for biodiesel production worldwide. winter rapeseed is commonly grown in the southern part of korea under a rice-rapeseed double cropping system. in this study, a greenhouse pot experiment was conducted to assess the effects of rapeseed residue applied as a green manure alone or in combinations with mineral n fertilizer on cd and pb speciation in the contaminated paddy soil and their availability to rice plant (oryza sativa l.). the changes in soi ...201121764102
selenium accumulation by forage and grain crops and volatilization from seleniferous soils amended with different organic materials.greenhouse and field experiments were conducted to study the direct and residual effect of applying different organic materials on se accumulation by crops and volatilization from seleniferous soils of northwestern india. among organic manures, press mud and poultry manures proved 4-5 times more effective in reducing se accumulation by different crops than farmyard manure. efficiency of organic manures increased with increase in application rates. application of both press mud and poultry manure ...201020015536
imidazolinone-tolerant crops: history, current status and future.imidazolinone herbicides, which include imazapyr, imazapic, imazethapyr, imazamox, imazamethabenz and imazaquin, control weeds by inhibiting the enzyme acetohydroxyacid synthase (ahas), also called acetolactate synthase (als). ahas is a critical enzyme for the biosynthesis of branched-chain amino acids in plants. several variant ahas genes conferring imidazolinone tolerance were discovered in plants through mutagenesis and selection, and were used to create imidazolinone-tolerant maize (zea mays ...200515627242
bioavailability of bound residue derived from 14c-labeled chlorsulfuron in soil and its mechanism of phytotoxicity.the bioavailability of bound residue (br) derived from 14c-labeled chlorsulfuron in soil and effect of the main components of the br on growth of rape (brassica napus) and rice (oryza sativa l.) were investigated. the results showed that the br with the concentration of 0.28 and 0.56 nmol/g air-dried soil, which was calculated by special radioactivity of 14c-labeled chlorsulfuron parent compound, resulted in significant depression effect on growth of rape seedling. it was assured that the main c ...200415137651
molecular cloning and characterization of a new na+/h+ antiporter gene from brassica napus.a new na+/h+ vacuolar antiporter gene from brassica napus was cloned. the full-length cdna of b. napus antiporter gene (bnnhx1, genbank acc. no. ay189676) was 1819 bp and contained a 1545 bp open reading frame encoding a protein of 515 amino acids. homology analysis and molecular modeling revealed that bnnhx1 strongly resembled other na+/h+ antiporter genes. its encoded protein belonged to a typical sodium/hydrogen exchanger superfamily and shared consensus sequences within predicted transmember ...200314756421
surface structure and properties of plant seed oil bodies.storage triacylglycerols (tag) in plant seeds are present in small discrete intracellular organelles called oil bodies. an oil body has a matrix of tag, which is surrounded by phospholipids (pl) and alkaline proteins, termed oleosins. oil bodies isolated from mature maize (zea mays) embryos maintained their discreteness, but coalesced after treatment with trypsin but not with phospholipase a2 or c. phospholipase a2 or c exerted its activity on oil bodies only after the exposed portion of oleosin ...19921560029
corrigendum: transcriptomic comparison between brassica oleracea and rice (oryza sativa) reveals diverse modulations on cell death in response to sclerotinia sclerotiorum. 201627721501
transcriptomic comparison between brassica oleracea and rice (oryza sativa) reveals diverse modulations on cell death in response to sclerotinia sclerotiorum.sclerotinia stem rot caused by sclerotinia sclerotiorum is a devastating disease of brassica crops, but not in rice. the leaves of a rice line, a partial resistant (r) and a susceptible (s) brassica oleracea pool that bulked from a resistance-segregating f2 population were employed for transcriptome sequencing before and after inoculation by s. sclerotiorum for 6 and 12 h. distinct transcriptome profiles were revealed between b. oleracea and rice in response to s. sclerotiorum. enrichment analys ...201627647523
tc1-like transposable elements in plant genomes.the tc1/mariner superfamily of transposable elements (tes) is widespread in animal genomes. mariner-like elements, which bear a ddd triad catalytic motif, have been identified in a wide range of flowering plant species. however, as the founding member of the superfamily, tc1-like elements that bear a dd34e triad catalytic motif are only known to unikonts (animals, fungi, and entamoeba).201424926322
genome-wide identification and analysis of the b3 superfamily of transcription factors in brassicaceae and major crop plants.the plant-specific b3 superfamily of transcription factors has diverse functions in plant growth and development. using a genome-wide domain analysis, we identified 92, 187, 58, 90, 81, 55, and 77 b3 transcription factor genes in the sequenced genome of arabidopsis, brassica rapa, castor bean (ricinus communis), cocoa (theobroma cacao), soybean (glycine max), maize (zea mays), and rice (oryza sativa), respectively. the b3 superfamily has substantially expanded during the evolution in eudicots pa ...201323377560
expression of brd1, a plant defensin from brassica rapa, confers resistance against brown planthopper (nilaparvata lugens) in transgenic rices.plant defensins are small (5-10 kda) basic peptides thought to be an important component of the defense pathway against fungal and/or bacterial pathogens. to understand the role of plant defensins in protecting plants against the brown planthopper, a type of insect herbivore, we isolated the brassica rapa defensin 1 (brd1) gene and introduced it into rice (oryza sativa l.) to produce stable transgenic plants. the brd1 protein is homologous to other plant defensins and contains both an n-terminal ...200919714315
plantgm: a database for genetic markers in rice (oryza sativa) and chinese cabbage (brassica rapa).the plant genetic map database (plantgm) has been developed as a web-based system which provides information about genetic markers in rice (oryza sativa) and chinese cabbage (brassica rapa). the database has three major parts and functions; (1) map search, (2) marker search, and (3) qtl search. at present, the database provides characterization information for about 3258 genetic markers. it has 2800 rflp and 112 qtl markers related to rice in addition to 321 rflp and 25 pcr-based markers for chi ...200819238232
phylogenetic relationships of 10 grass species: an assessment of phylogenetic utility of the internal transcribed spacer region in nuclear ribosomal dna in monocots.entire sequences of the internal transcribed spacers (itss) and 5.8s subunit of nuclear ribosomal dna (nrdna) were obtained from nine grass species by direct double-stranded sequencing of polymerase chain reaction (pcr) amplified dna fragments. these sequences from subfamily pooideae (triticum aestivum, crithodium monococcum, sitopsis speltoides, hordeum vulgare, secale montanum, avena longiglumis, bromus inermis, brachypodium distachyon) and subfamily panicoideae (sorghum bicolor) together with ...19948181731
archaeobotanical study of ancient food and cereal remains at the astana cemeteries, xinjiang, china.starch grain, phytolith and cereal bran fragments were analyzed in order to identify the food remains including cakes, dumplings, as well as porridge unearthed at the astana cemeteries in turpan of xinjiang, china. the results suggest that the cakes were made from triticum aestivum while the dumplings were made from triticum aestivum, along with setaria italica. the ingredients of the porridge remains emanated from panicum miliaceum. moreover, direct macrobotantical evidence of the utilization o ...201223028807
constitutive expression of capla1 conferred enhanced growth and grain yield in transgenic rice plants.phospholipids are not only important components of cell membranes, but participate in diverse processes in higher plants. in this study, we generated capsicum annuum phospholipiase a1 (capla1) overexpressing transgenic rice (oryza sativa l.) plants under the control of the maize ubiquitin promoter. the t4 capla1-overexpressing rice plants (ubi:capla1) had a higher root:shoot mass ratio than the wild-type plants in the vegetative stage. leaf epidermal cells from transgenic plants had more cells t ...201626803502
capub1, a hot pepper u-box e3 ubiquitin ligase, confers enhanced cold stress tolerance and decreased drought stress tolerance in transgenic rice (oryza sativa l.).abiotic stresses such as drought and low temperature critically restrict plant growth, reproduction, and productivity. higher plants have developed various defense strategies against these unfavorable conditions. capub1 (capsicum annuum putative u-box protein 1) is a hot pepper u-box e3 ub ligase. transgenic arabidopsis plants that constitutively expressed capub1 exhibited drought-sensitive phenotypes, suggesting that it functions as a negative regulator of the drought stress response. in this s ...201626674966
determination of the source of bioavailable sr using ⁸⁷sr/⁸⁶sr tracers: a case study of hot pepper and rice.the geographical origin of agricultural products has been intensively studied, but links between agricultural products and the environments are poorly established. soils, water (streamwater and groundwater), and plants (hot pepper, capsicum annuum; and rice, oryza sativa) were collected from all regions of south korea and measured sr isotope ratios ((87)sr/(86)sr). sequential leaching of soil showed that sr in the exchangeable and carbonate fractions (bioavailable) had a lower (87)sr/(86)sr rati ...201425186083
molecular cloning of indian jujube (zizyphus mauritiana) allergen ziz m 1 with sequence similarity to plant class iii chitinases.indian jujube (zizyphus mauritiana) is a sweet fruit that is abundantly cultivated in taiwan. we have previously identified 42 and 30 kda allergens that are cross-reactive with latex allergen from crude indian jujube extract. this study aimed to clone the 30 kda ziz m 1 z. mauritiana allergen. the ziz m 1 encoding cdna was isolated from a zapii cdna library constructed from z. mauritiana mrna, sequenced and expressed in pichia pastoris. the protein predicted from the cdna sequence has 330 amino ...200616122801
a new aox homologous gene osim1 from rice (oryza sativa l.) with an alternative splicing mechanism under salt stress.a differentially expressed osim1 gene was isolated from rice salt-tolerant mutant m-20 by differential display. sequence analysis revealed that the amino-acid sequence of osim1 showed 66% and 62% identity with ptox from tomato ( capsicum annuum) and atim from arabidopsis, both of which encoded chloroplast-orientated terminal oxidase. comparison of the nucleotide sequence of the osim1 cdna with its genomic sequence revealed that osim1 genomic dna contained nine exons and eight introns. a pseudo-t ...200312669200
the effects of a selection of alkaloids on the invertase activity of some higher plants.the effects of several alkaloids on the invertase activities of ricinus communis, solanum tuberosum, oryza sativa and carica papaya were studied. the following alkaloids: caffeine, berberine, strychnine, morphine, ethyl-narceine and nornicotine, have different inhibitory effects on invertase activity. kinetic studies of the alkaloid inhibition revealed that different alkaloids exhibited diverse types of inhibition of invertase. it was found that alkaloids form reversible complexes with invertase ...19979442928
influence of sodium selenite on growth, nutrient utilization and selenium uptake in cavia porcellus.a 70 day experiment on forty guinea pigs (cavia porcellus) was conducted to find the influence of different level of sodium selenite (inorganic selenium supplementation) on growth, nutrient utilization and selenium uptake. the sodium selenite was supplemented into a basal diet at 0, 0.1, 0.2 and 0.3 ppm, respectively and the basal diet comprised of 25% ground cowpea (vigna unguiculata) hay, 30% ground maize (zea mays) grain, 22% ground gram (cicer arietinum) grain, 9.5% deoiled rice (oryza sativ ...201224163954
photoinhibition at low temperature in chilling-sensitive and -resistant plants.photoinhibition resulting from exposure at 7 degrees c to a moderate photon flux density (300 micromoles per square meter per second, 400-700 nanometers) for 20 hours was measured in leaves of annual crops differing widely in chilling tolerance. the incidence of photoinhibition, determined as the decrease in the ratio of induced to total chlorophyll fluorescence emission at 693 nanometers (f(v)/f(max)) measured at 77 kelvin, was not confined to chilling-sensitive species. the extent of photoinhi ...198916666971
protein content and amino acids profile of pseudocereals.quinoa (chenopodium quinoa), amaranth (amaranthus caudatus) and buckwheat (fagopyrum esculentum) represent the main protein source in several diets, although these pseudocereals are not currently present in the fcdb nutrient profile information. the aim of this work is to characterise the aa profile of these pseudocereals and compare them with rice. total protein content revealed to vary from 16.3g/100g (quinoa salta) to 13.1g/100g (buckwheat) and lower values were found in rice samples (6.7g/10 ...201626433287
antixenosis and antibiosis resistance in rice cultivars against chilo suppressalis (walker) (lepidoptera: crambidae).the striped stem borer, chilo suppressalis (walker) (lepidoptera: crambidae), is an important pest afflicting rice in most rice-growing countries in the world. deliniating the categories of resistance in rice genotypes under field conditions could be helpful in managment of this pest. two categories of resistance, antixenosis and antibiosis, were examined in ten popular and diverse rice genotypes of different origin that had been selected for their resistance to the striped stem borer in a previ ...201628039677
combined transcriptome and metabolome analyses to understand the dynamic responses of rice plants to attack by the rice stem borer chilo suppressalis (lepidoptera: crambidae).rice (oryza sativa l.), which is a staple food for more than half of the world's population, is frequently attacked by herbivorous insects, including the rice stem borer, chilo suppressalis. c. suppressalis substantially reduces rice yields in temperate regions of asia, but little is known about how rice plants defend themselves against this herbivore at molecular and biochemical level.201627923345
the overexpression of insect endogenous small rnas in transgenic rice inhibits growth and delays pupation of striped stem borer (chilo suppressalis).the striped stem borer (ssb), chilo suppressalis walker, is a major rice insect pest worldwide. rna interference (rnai) has become a promising strategy for developing insect-resistant crops. in a previous study, five double-stranded rnas (dsrnas) targeting important ssb housekeeping genes were overexpressed in rice, but none of the acquired dsrna-transgenic rice plants showed significant effects on ssb.201627862861
the rice transcription factor wrky53 suppresses herbivore-induced defenses by acting as a negative feedback modulator of mitogen-activated protein kinase activity.the mechanisms by which herbivore-attacked plants activate their defenses are well studied. by contrast, little is known about the regulatory mechanisms that allow them to control their defensive investment and avoid a defensive overshoot. we characterized a rice (oryza sativa) wrky gene, oswrky53, whose expression is rapidly induced upon wounding and induced in a delayed fashion upon attack by the striped stem borer (ssb) chilo suppressalis. the transcript levels of oswrky53 are independent of ...201526453434
characterization of competitive interactions in the coexistence of bt-transgenic and conventional rice.transgene flow through pollen and seeds leads to transgenic volunteers and feral populations in the nature, and consumer choice and economic incentives determine whether transgenic crops will be cultivated in the field. transgenic and non-transgenic plants are likely to coexist in the field and natural habitats, but their competitive interactions are not well understood.201525928331
seasonal expression of bt proteins in transgenic rice lines and the resistance against asiatic rice borer chilo suppressalis (walker).laboratory bioassays and field surveys were carried out to compare the resistance of three transgenic rice (oryza sativa l.) lines including bt-dl expressing a single gene cry1ab, bt-kf6 expressing stacked genes cry1ac and cpti genes and bt-sy63 expressing a fusion gene cry1ab/cry1ac, respectively, to an important rice pest chilo suppressalis (walker). in addition, enzyme-linked immunosorbent assays (elisa) were conducted to monitor the bt protein expressions in rice leaves and stems at differen ...201122251743
an ear-motif-containing erf transcription factor affects herbivore-induced signaling, defense and resistance in rice.ethylene responsive factors (erfs) are a large family of plant-specific transcription factors that are involved in the regulation of plant development and stress responses. however, little to nothing is known about their role in herbivore-induced defense. we discovered a nucleus-localized erf gene in rice (oryza sativa), oserf3, that was rapidly up-regulated in response to feeding by the rice striped stem borer (ssb) chilo suppressalis. antisense and over-expression of oserf3 revealed that it po ...201121831212
silicon-mediated rice plant resistance to the asiatic rice borer (lepidoptera: crambidae): effects of silicon amendment and rice varietal resistance.the asiatic rice borer, chilo suppressalis (walker) (lepidoptera: crambidae), is one of the most destructive pests in rice, oryza sativa l., throughout asian countries. the aim of this study was to investigate the potential of applied silicon in mediating rice plant resistance to c. suppressalis in a susceptible (shanyou63) and a moderately resistant (yanfeng47) rice cultivar. silicon-treated plants showed significant increases in silicon content compared with the control. silicon addition signi ...201020857756
induction of serotonin accumulation by feeding of rice striped stem borer in rice leaves.tryptophan (trp)-related secondary metabolism has been implicated in the defense against pathogen infection and insect feeding in various gramineous species. recently, we also reported that rice plant accumulated serotonin and tryptamine as well as their amide compounds coupled with phenolic acids in response to the infection by fungal pathogen. these compounds were likely to play an important role in the formation of physical barrier to the invading pathogens. to extend our study to elucidate t ...200819704837
changes in levels of juvenile hormone and molting hormone in larvae and adult females of chilo suppressalis (lepidoptera: pyralidae) after imidacloprid applications to rice.insect hormones regulate growth and development and fecundity of insects. the current study investigated changes in juvenile hormone (jh) and molting hormone (mh) levels in fourth instars and adult females of chilo suppressalis (walker) (lepidoptera: pyralidae) after imidacloprid application to rice, oryza sativa l. the results showed that jh level in fourth instars that developed feeding on fengyouxiangzhan rice plants sprayed with 15, 30, and 60 ppm imidacloprid was significantly higher than t ...200717849869
distribution of imidacloprid residues in different parts of rice plants and its effect on larvae and adult females of chilo suppressalis (lepidoptera: pyralidae).the distribution of imidacloprid residue in different parts of rice, oryza sativa l. (graminales: poaceae), plants was investigated for three rice varieties. changes in larval hormones in chilo suppressalis (walker) (lepidoptera: pyralidae) that fed on imidacloprid-treated plant sources and the fecundity of resultant adult females (moths) also were determined. no significant differences in imidacloprid residue were detected in rice stem, leaves, and the unexpanded new leaf 7 d after treatment ex ...200717461061
utilization of extrusion technology for the development of millet based complementary foods.millet based complementary foods were developed using sorghum (sorghum vulgare), rice (oryza sativa), besan (cicer arietinum; bengal gram dhal flour), legume mix (green gram and roasted bengal gram; phaseolus aureus roxb and cicer arietinum) and soybean (glycine max merr) with a lab scale twin screw extruder. the extrudates were subjected to acceptability studies initially and at the end of the storage period i.e. 3 months at laboratory level by panel of judges using a 5-point hedonic scale. phy ...201425328236
a computational analysis on lectin and histone h1 protein of different pulse species as well as comparative study with rice for balanced diet.the issue of balanced nutrition is of great concern to human. meat and fish are the best sources of protein. the affordability of these resources for people in developing countries is less. thus, there is an increasing interest in pulses and its derivates as an alternative to fish and meat. lectin and histone h1 are the most common proteins in various pulses and our interest is in identifying the dominant essential amino acids in them for use as supplements. however, actin and lectin are common ...201222419840
multifunctional crop trait ontology for breeders' data: field book, annotation, data discovery and semantic enrichment of the literature.agricultural crop databases maintained in gene banks of the consultative group on international agricultural research (cgiar) are valuable sources of information for breeders. these databases provide comparative phenotypic and genotypic information that can help elucidate functional aspects of plant and agricultural biology. to facilitate data sharing within and between these databases and the retrieval of information, the crop ontology (co) database was designed to provide controlled vocabulary ...201022476066
characterization of the genes encoding the cytosolic and plastidial forms of adp-glucose pyrophosphorylase in wheat endosperm.in most species, the synthesis of adp-glucose (glc) by the enzyme adp-glc pyrophosphorylase (agpase) occurs entirely within the plastids in all tissues so far examined. however, in the endosperm of many, if not all grasses, a second form of agpase synthesizes adp-glc outside the plastid, presumably in the cytosol. in this paper, we show that in the endosperm of wheat (triticum aestivum), the cytosolic form accounts for most of the agpase activity. using a combination of molecular and biochemical ...200212428011
accurate measurement of transgene copy number in crop plants using droplet digital pcr.genetic transformation is a powerful means for the improvement of crop plants, but requires labor- and resource-intensive methods. an efficient method for identifying single-copy transgene insertion events from a population of independent transgenic lines is desirable. currently, transgene copy number is estimated by either southern blot hybridization analyses or quantitative polymerase chain reaction (qpcr) experiments. southern hybridization is a convincing and reliable method, but it also is ...201728231382
ratoon rice generated from primed parent plants exhibit enhanced herbivore resistance.rice ratooning is practiced in many rice-growing countries for achieving increased rice production with limited labour input. here, we report that attack by insect herbivores, or treatment with a defense signaling compound in parent plants, can prime anti-herbivore defense responses in subsequent ratoon plants. we compared the defense responses of rice ratoons generated from parent plants that had been either infested by cnaphalocrocis medinalis (rice leaffolder, lf) caterpillars or treated with ...201728042888
priming of jasmonate-mediated antiherbivore defense responses in rice by silicon.although the function of silicon (si) in plant physiology has long been debated, its beneficial effects on plant resistance against abiotic and biotic stresses, including insect herbivory, have been well documented. in addition, the jasmonate (ja) signaling pathway plays a crucial role in mediating antiherbivore defense responses in plants. however, potential interactions between ja and si in response to insect attack have not been examined directly. to explore the role ja may play in si-enhance ...201324003150
resistance of cultivated rice varieties to cnaphalocrocis medinalis (lepidoptera: pyralidae).cnaphalocrocis medinalis guenée (lepidoptera: pyralidae) is a major pest in rice, oryza sativa l. (graminales: poaceae), in asia. the current study investigated the resistance of 17 rice varieties or lines to c. medinalis and behavioral responses of the insect to varieties of different corrected damage ratings (cdrs) and damaged leaves scales (dlss). the results showed that most varieties (or lines) commonly cultured in rice production were susceptible (dls 3 and 5) to damage caused by c. medina ...201020857724
molecular and biochemical markers associated with leaffolder (cnaphalocrocis medinalis g.) resistance in rice (oryza sativa l.).association of molecular markers namely isozymes and simple sequence repeats (ssrs) and various biochemical markers to leaffolder (cnaphalocrocis medinalis g., a predominant insect pest of rice) resistance were studied in rice (oryza sativa l.). recombinant inbred lines (rils) of f8 generation obtained by crossing ir36 (susceptible parent) and tnaulfr831311 (moderately resistant parent) were used in this study. soluble protein content, protein profile, and peroxidase and phenylalanine ammonia ly ...200523923546
abiotic stresses affect differently the intron splicing and expression of chloroplast genes in coffee plants (coffea arabica) and rice (oryza sativa).despite the increasing understanding of the regulation of chloroplast gene expression in plants, the importance of intron splicing and processing of chloroplast rna transcripts under stress conditions is largely unknown. here, to understand how abiotic stresses affect the intron splicing and expression patterns of chloroplast genes in dicots and monocots, we carried out a comprehensive analysis of the intron splicing and expression patterns of chloroplast genes in the coffee plant (coffea arabic ...201627448724
ogro: the overview of functionally characterized genes in rice online database.the high-quality sequence information and rich bioinformatics tools available for rice have contributed to remarkable advances in functional genomics. to facilitate the application of gene function information to the study of natural variation in rice, we comprehensively searched for articles related to rice functional genomics and extracted information on functionally characterized genes.201227234245
how to isolate a plant's hypomethylome in one shot.genome assembly remains a challenge for large and/or complex plant genomes due to their abundant repetitive regions resulting in studies focusing on gene space instead of the whole genome. thus, dna enrichment strategies facilitate the assembly by increasing the coverage and simultaneously reducing the complexity of the whole genome. in this paper we provide an easy, fast, and cost-effective variant of mre-seq to obtain a plant's hypomethylome by an optimized methyl filtration protocol followed ...201526421293
the study of the e-class sepallata3-like mads-box genes in wild-type and mutant flowers of cultivated saffron crocus (crocus sativus l.) and its putative progenitors.to further understand flowering and flower organ formation in the monocot crop saffron crocus (crocus sativus l.), we cloned four mikc(c) type ii mads-box cdna sequences of the e-class sepallata3 (sep3) subfamily designated csatsep3a/b/c/c_as as well as the three respective genomic sequences. sequence analysis showed that cdna sequences of csatsep3 c and c_as are the products of alternative splicing of the csatsep3c gene. bioinformatics analysis with putative orthologous sequences from various p ...201121621873
isolation and functional characterization of cslsi1, a silicon transporter gene in cucumis sativus.cucumber (cucumis sativus) is a widely grown cucurbitaceous vegetable that exhibits a relatively high capacity for silicon (si) accumulation, but the molecular mechanism for silicon uptake remains to be clarified. here we isolated and characterized cslsi1, a gene encoding a silicon transporter in cucumber (cv. mch-4). cslsi1 shares 55.70 and 90.63% homology with the lsi1s of a monocot and dicot, rice (oryza sativa) and pumpkin (cucurbita moschata), respectively. cslsi1 was predominantly expresse ...201727701737
synthesis and herbicidal activity of 12-(aryloxyacyloxyimino)-1,15-pentadecanlactone derivatives.a series of novel 12-(aryloxyacyloxyimino)-1,15-pentadecanlactone derivatives (3) were synthesized, and their structures including configuration of c=n bond were confirmed by (1)h nmr, elemental analysis and x-ray diffraction analysis. the bioassay showed that some of them exhibited excellent herbicidal activity against amaranthus tricolor l. the activity of compounds 3 except compounds 3a1-2 was much higher than the commercial herbicide 2,4-d and the activity of about half of compounds 3 was co ...200919117417
germanium-68 as an adequate tracer for silicon transport in plants. characterization of silicon uptake in different crop species.a basic problem in silicon (si) uptake studies in biology is the lack of an appropriate radioactive isotope. radioactive germanium-68 ((68)ge) has been used previously as a si tracer in biological materials, but its suitability for the study of si transport in higher plants is still untested. in this study, we investigated (68)ge-traced si uptake by four crop species differing widely in uptake capacity for si, including rice (oryza sativa), barley (hordeum vulgare), cucumber (cucumis sativus), a ...200717098850
synthesis and herbicidal activity of n,n-diethyl-3-(arylselenonyl)-1h-1,2,4-triazole-1-carboxamide.based on the carbamoyl triazole herbicide cafenstrole, 12 novel selenium-containing compounds were designed and synthesized. all of the compounds were characterized and confirmed by ir, 1h nmr, and high-resolution mass spectroscopy. the bioassay tests showed that some of the compounds (c2, c4, c(7-8), and c12) exhibited good inhibitory activity against cucumber (cucumis sativus l.) and semen euphorbiae (leptochloa chinensis n.). especially, compound c6 inhibited the growth of cucumber and semen ...200617002445
effects of 3-methylgibberellin analogs on gibberellin 3β-hydroxylases and plant growth.six 3-methylgibberellin analogs were synthesized, and their effects on the ga 3β-hydroxylases from immature seeds of phaseolus vulgaris and cucurbita maxima, and/or on the growth of dwarf rice (oryza sativa l. cv. tan-ginbozu) and cucumber (cucumis sativus l. cv. spacemaster) were investigated. 3-methyl-ga5 and 2, 3-didehydro-3-methyl-ga9· inhibited the conversion of [2, 3-(3)h2]ga9 to [2-(3)h]ga4 by ga 3β-hydroxylases from both p. vulgaris and c. maxima at 3 μm and higher. their c/d-ring-rearra ...199227286375
physiology and bio - chemistry of germination of different types of seeds - iv - effect of certain chemicals on growth anddevelopment of cucumber, mungo, paddy, raddish and tomato plants.the effect of 100 ppm solution of each of kinetin, adenine, uracil and thymine on the vegetative and reproductive growth of cucumis sativus, phaseolus mungo, oryza sativus, raphanus sativus and lycopersicum esculentum plants were studied. the rate of vegetative growth was found to be more in the cucumis sativus, raphanus sativus and lycopersicum esculentum plants treated with all the chemicals mentioned above over that of the controls. phaseolus mungo and oryza sativa plants shows almost the sam ...198422557413
destination-selective long-distance movement of phloem proteins.the phloem macromolecular transport system plays a pivotal role in plant growth and development. however, little information is available regarding whether the long-distance trafficking of macromolecules is a controlled process or passive movement. here, we demonstrate the destination-selective long-distance trafficking of phloem proteins. direct introduction, into rice (oryza sativa), of phloem proteins from pumpkin (cucurbita maxima) was used to screen for the capacity of specific proteins to ...200515863519
nutritional, sensory and physical analysis of pumpkin flour incorporated into weaning mix.the objective of this study was to develop a cereal-pulse complementary food fortified with different concentrations of pumpkin powder (cucurbita moschata), and to analyse its sensory and physic-chemical parameters. fresh pumpkins(cucurbita moschata) were procured from the market and dehydrated and powdered in the laboratory. sorghum (sorghum vulgare) and whole green gram (vigna radiate) were germinated, dried, pulverised and combined with powdered rice (oryza sativa) in the ratio of 2:1:1. pump ...201022691991
in vitro synthesis of curcuminoids by type iii polyketide synthase from oryza sativa.curcuminoids, major components of the spice turmeric, are used as a traditional asian medicine and a food additive. curcumin, a representative curcuminoid, has received a great deal of attention because of its anti-inflammatory, anticarcinogenic, and antitumor activities. here we report a novel type iii polyketide synthase named curcuminoid synthase from oryza sativa, which synthesizes bisdemethoxycurcumin via a unique mechanism from two 4-coumaroyl-coas and one malonyl-coa. the reaction begins ...200717932040
investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed tissue.we report the investigation of an endosperm-specific promoter from the rsus3 gene from rice (oryza sativa). the promoter was characterized by deletion analysis and transient expression in guar (cyamopsis tetragonoloba) seed-tissue. transient expression was monitored by histochemical gus assay, and quantitative dual reporter assays comprising firefly luciferase as a test reporter, and renilla luciferase and gus as reference reporters. these revealed high expression levels of the reporter genes di ...200616670901
molecular characterization and in silico analysis of sorghum panallergens: profilin and polcalin.in india, 20-30% of the human population suffer from allergic rhinitis and 15% of them develop asthma. plant pollens are one of the causative aeroallergens and are mixture of a number of molecules including major and minor allergens (panallergens). profilin and polcalcin are the known pollen specific panallergens. allergenicity of the sorghum plant in andhra pradesh was found to be 54.9%. but the allergens responsible have not been characterized well. this study highlights identification and mol ...201526669015
immunoglobulin e-binding reactivities of natural pollen grain extracts from selected grass species in the philippines.pollen grains have been reported to be present in the philippine atmosphere but studies regarding their allergenicity are limited.201222701864
description and sem observations of meloidogyne sasseri n. sp. (nematoda: meloidogynidae), parasitizing beachgrasses.meloidogyne sasseri n. sp. is described and illustrated from american beachgrass (ammophila breviliffulata) originally collected from henlopen state park and fenwick island near the maryland state line in delaware, united states (6). its relationship to m. graminis, m. spartinae, and m. californiensis is discussed. primary distinctive characters of the female perineal pattern were a high to rounded arch with shoulders, widely spaced lateral lines interrupting transverse striations, a sunken vulv ...199319279820
a genetic linkage map of tetraploid orchardgrass (dactylis glomerata l.) and quantitative trait loci for heading date.orchardgrass (dactylis glomerata l.), or cocksfoot, is indigenous to eurasia and northern africa, but has been naturalized on nearly every continent and is one of the top perennial forage grasses grown worldwide. to improve the understanding of genetic architecture of orchardgrass and provide a template for heading date candidate gene search in this species, the goals of the present study were to construct a tetraploid orchardgrass genetic linkage map and identify quantitative trait loci associa ...201222551303
characterization of allergenic epitopes of ory s1 protein from oryza sativa and its homologs.vaccination is the most effective technique suggested now days for allergy treatment. recombinant-based approaches are mostly focused on genetic modification of allergens to produce molecules with reduced allergenic activity and conserved antigenicity. the molecules developed for vaccination in allergy possess significantly reduced allergenicity in terms of ige binding, and therefore will not lead to anaphylactic reactions upon injection. this approach is probably feasible with every peptide all ...200920011147
cross-species amplification tests and diversity analysis using 56 pcr markers in dactylis glomerata and lolium perenne.we report results of cross-species amplification in dactylis glomerata and lolium perenne of 12 simple sequence repeats (ssrs) isolated from lolium multiflorum×festuca glaucescens, 42 ssrs from festuca arundinacea and two sequence tagged sites from oryza sativa. we compared the transferability and diversity between d. glomerata and l. perenne, which are important forage crops. while nei's gene diversity values were equivalent in both species (from 0.14 to 0.92), the mean number of allele per loc ...200921564590
expression of a heterologous s-adenosylmethionine decarboxylase cdna in plants demonstrates that changes in s-adenosyl-l-methionine decarboxylase activity determine levels of the higher polyamines spermidine and spermine.we posed the question of whether steady-state levels of the higher polyamines spermidine and spermine in plants can be influenced by overexpression of a heterologous cdna involved in the later steps of the pathway, in the absence of any further manipulation of the two synthases that are also involved in their biosynthesis. transgenic rice (oryza sativa) plants engineered with the heterologous datura stramonium s-adenosylmethionine decarboxylase (samdc) cdna exhibited accumulation of the transgen ...200212177487
production and analysis of asymmetric hybrid plants between monocotyledon (oryza sativa l.) and dicotyledon (daucus carota l.).asymmetric hybrid plants were obtained from fused protoplasts of a monocotyledon (oryza sativa l.) and a dicotyledon (daucus carota l.). x-ray-irradiated protoplasts isolated from a cytoplasmic malesterile (cms) carrot suspension culture were fused with iodoacetoamide-treated protoplasts isolated from a 5-methyltryptophan (5mt)-resistant rice suspension culture by electrofusion. the complementary recovered cells divided and formed colonies, which were then cultivated on regeneration medium suppl ...199424177855
selection and nuclear dna analysis of cell hybrids between daucus carota and oryza sativa.in order to study the fate of the parental genomes in somatic cell hybrids between distantly related species, protoplasts from cultured cells of daucus carota and oryza sativa were fused. selective conditions resulted, exclusively, in the growth of hybrid colonies which combined the capacity to multiply of carrot cells with the natural resistance to a2ca of rice cells. a methodology for measuring the relative contribution of the parental cells to the hybrid nuclear genome has been worked out. th ...198523196174
inactivation of aphidicolin by plant cells.plant cells are endowed with an aphidicolin inactivating activity. data on cultured cells show that the rate of inactivation depends on the cell type, daucus carota cells being the most effective among the other tested materials (oryza sativa and nicotiana plumbaginifolia). also germinating seedling of haplopappus gracilis and of citrullus vulgaris inactivate aphidicolin.inactivation, which may lead to unexpected results when a prolonged incubation with the drug is required, as in the case of th ...198324258125
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