| organized nerve culture. i. a technique to study the effect of m. leprae infection. | neonatal dorsal root ganglia were cultivated in vitro by the technique of murray. within a week bundles of organized nerve fibers containing proliferating schwann cells in different phases of axon association and fibroblast cells destined to become peri- or endoneural cells were obtained, peri- or endoneural cells were obtained. many of these nerve fibers were myelinated within 3-4 weeks. such 1 or 2 week old cultures were inoculated with m. leprae, and bacilli were found in the cytoplasm of sch ... | 1980 | 6995359 |
| organized nerve culture. ii. dna synthesis in schwann cells in the presence of m. leprae. | schwann cells that contain m. leprae fail to incorporate dna precursor, indicating blockage of dna synthesis. such a block could lead to no proliferation of schwann cells, an essential requirement for successful association with axons and consequent myelination. | 1980 | 6995360 |
| significance of immune reactions in leprosy. | | 1980 | 6995566 |
| immunology of leprosy. | | 1980 | 6996917 |
| delineation of a human t cell subset responsible for lepromin-induced suppression in leprosy patients. | | 1980 | 6997379 |
| immune response to mycobacterium leprae: further studies on the assessment of humoral immune response in mice. | immuncyte proliferation in the spleens of mice given both a primary and a second infection sixty days later, was detected soon after the second challenge was administered. plaque-forming cell assay for both the direct and developed plaques indicated that all cells producing antibody of both immunoglobulin classes were present in the animals when they were administered the second challenge. hemagglutinating antibody determinations indicated that igg antibodies are recognizable at a time when the ... | 1980 | 6999803 |
| effect of purification steps on the immunogenicity of mycobacterium leprae. | in studies aimed at the development of an antileprosy vaccine for use in man, mycobacterium leprae suspensions were prepared from livers of experimentally infected armadillos. the 2 methods of purification in chief use, carried out after irradiation of the tissue with 2.5 megarads of gamma irradiation from 60co, involved treatment with 0.1n naoh for 2 h at room temperature, trypsin and chymotrypsin digestion for 24h at 37 degrees, and separation in a 2-phase liquid polymer (dextran:polyethylene ... | 1980 | 7000133 |
| searches among mycobacterial cultures for antileprosy vaccines. | all mycobacteria species share some antigens, so there may be cultivable mycobacterial cultures that can provide vaccine protection against leprosy. vaccine protection against mycobacterium leprae infections in mice has been demonstrated for m. leprae itself, as living or heat-killed suspensions, and for mycobacterium bovis (bcg), as living suspensions. results are reported here with 17 other cultures. the mycobacterial suspensions were injected intradermally, and the mice were challenged in the ... | 1980 | 7000701 |
| the suppressive effect of m. leprae on the in vitro proliferative responses of lymphocytes from patients with leprosy. | peripheral blood lymphocytes from sixty leprosy patients and eight healthy contacts known to be responsive to m. leprae, were stimulated in vitro with concanavalin a (con a) or ppd alone or in combination with autoclaved, whole m. leprae. time kinetics and the percentage of inhibition induced by m. leprae differed in the two disease groups and contacts. antigen-generated suppression of con a-stimulated lymphocyte transformation was observed on day 4 in seventeen of twenty-one (80%) tuberculoid p ... | 1980 | 7002396 |
| viability of mycobacterium leprae after multiplication in mice. | to measure the rate at which mycobacterium leprae are killed in the course of the mouse footpad infection after the maximum of multiplication has been achieved, m. leprae were harvested shortly before and at intervals after multiplication had reached the level of 10(6) organisms per footpad, serially diluted, and inoculated into the footpads of passage mice. beginning 1 year later, foot-by-foot harvests of m. leprae were performed from passage mice, and the proportion of viable organisms in the ... | 1980 | 7002795 |
| comparative studies of antigen 21 in mycobacterium and nocardia species: possible taxonomic relationships with mycobacterium leprae. | studies of mycobacterium leprae, mycobacterium tuberculosis and nocardia caviae in comparison with each other and with other mycobacterium and nocardia species were performed on the basis of antigen 21 intramolecular heterogeneity. three different antisera were used: rabbit anti-mycobacterium smegmatis antiserum, rabbit anti-nocardia asteroides antiserum, and a lepromatous serum pool. with reference to each of the three antiserum sources used the strains were ranked in an order of relatedness or ... | 1980 | 7002798 |
| detection of antigen in nocardia caviae cross-reacting with mycobacterial antigen no. 21 in m. leprae using a lepromatous leprosy serum pool as antibody reagent. | eighteen strains of nocardia, representing five different species, were studied in crossed immunoelectrophoresis against a lepromatous leprosy serum pool for the presence of antigen no. 21. this mycobacterial antigen shows antigenic heterogeneity with species specific antigenic determinants defined in mycobacterium leprae. all four strains of n. caviae were found to share antigen no. 21 determinants with mucobacteria. all other strains of nocardia were negative in these direct immunoprecipitatio ... | 1980 | 7002810 |
| combined rifampin and dapsone chemotherapy of mycobacterium leprae infection of the neonatally thymectomized lewis rat. | neonatally thymectomized lewis rats (ntlr) were shown to be highly susceptible to infection with mycobacterium leprae. we have used them in chemotherapeutic studies as models of human lepromatous leprosy. ntlr chronically infected with m. leprae were treated with various regimens combining a background of the minimal effective dose (med) of dapsone (4,4'-diaminodiphenylsulfone, dds) or 100 times this dose in the diet with one to ten doses of rifampin (rmp) of 10 mg/kg. to test for persisting via ... | 1980 | 7002811 |
| the transmission of leprosy in man. | existing clinical, scientific, and epidemiological knowledge on the mode of transmission of human leprosy is reviewed under the following headings: a. the release of viable organisms from the host into the environment. b. the presence of viable organisms so released into the environment. c. entry into the new human host and distribution within the body. d. production of clinical illness. it is concluded that much of the published evidence deals with one, or rather few, parameters, whose relation ... | 1980 | 7002813 |
| effect of purification procedures on the viability of mycobacterium leprae. | | 1980 | 7002817 |
| [control of leprosy: fantasies and possibilities]. | | 1980 | 7004552 |
| conversion of reactions to leprolin and lepromin in patients with lepromatous leprosy by the transfer factor. | conversion of leprolin and early lepromin reactions was achieved by two injections of transfer factor made of lymphocytes from lepromin-positive tuberculoid leprosy patients. however, the late reaction to lepromin remained unchanged. the importance of the degree of sensitivity of the cell donor was demonstrated, and a booster dose was also found to be useful. the feasibility of using transfer factor in treatment of lepromatous leprosy is briefly discussed. | 1980 | 7004800 |
| macrophage culture from untreated leprosy cases. | macrophage cultures from the peripheral blood were performed in 25 untreated bacteraemic leprosy patients. the monocytes on conversion to macrophages in culture continued to harbour solidly stained m. leprae till the termination of culture. it is suggested that the majority of these bacilli are viable and capable of multiplication intracellularly in macrophages if cultures could be maintained for sufficiently long periods. it was observed that bacteraemia in leprosy can be detected by the cultur ... | 1980 | 7005539 |
| life cycle of mycobacterium leprae. | | 1980 | 7005541 |
| secondary sulphone resistance in leprosy (report of a case). | a case of secondary sulphone resistant lepromatous leprosy proved by mouse foot-pad tests is reported from pondicherry, india. | 1980 | 7005542 |
| antibodies against mycobacterium leprae antigen 7 from birth to 18 months of age: an indicator of intra-uterine infection in leprosy. | all babies of three non-leprosy mothers and ten tuberculoid leprosy mothers and four of five babies of mothers with inactive lepromatous leprosy showed a decline in serum concentration of antibodies against m. leprae antigen 7 during the first 4 months of life, as expected from catabolism of maternal igg. by contrast, ten of twenty babies of mothers with active lepromatous leprosy showed a decline in concentration of anti-m. leprae 7 antibodies considerably less than expected. this indicates tha ... | 1980 | 7006873 |
| bcg, corynebacterium parvum or mycobacterium leprae added to cultures of bcg-primed mouse spleen cells cause an enhanced primary antibody response in vitro. | a few weeks after mice were injected i.v. with 10(8) live mycobacterium bovis, bcg, the antibody response of their spleen cells to srbc in vitro was comparable with the response of cells from untreated mice. addition of bcg organisms to the culture vessels resulted in enhanced antibody-forming cell (afc) responses by the primed cells but not by the cells from the untreated mice. no evidence was found for a direct stimulation of b cells and cell depletion experiments suggested macrophages were di ... | 1981 | 7007221 |
| cultivable precursors of mycobacterium leprae. | during attempts at test tube culture of m. leprae in our laboratory we have repeatedly isolated a non-acid fast, coccoid organism from lepromatous tissue and skin smears (chatterjee, 1976). these organisms show a tendency to generate acid fast mycobacteria in test tube passages and in mice experimentally infected with these organisms, and a number of stable, pigmented mycobacterial cultures have been obtained from them that are being maintained in test tube media. in the first report cited above ... | 1980 | 7007724 |
| choice of skin slit smears for study of bacterial and morphological indices. | skin slit smears of 46 patients of lepromatous leprosy (16 untreated, 30 long treated) were studied from ear lobules, fingers, elbows and knees. in untreated patients ear lobules gave highest bi, as compared to other sites. mi from ear lobules was higher than elbows and knees but slightly lower than that from fingers. in treated patients sites other than ear lobules yielded solid staining bacilli more frequently. multiple sites and especially peripheral sites are recommended for study of skin sl ... | 1980 | 7007725 |
| third annual report of the undp/world bank/who special programme for research and training in tropical diseases. | | 1980 | 7007726 |
| demonstration of antibodies against mycobacterium leprae both in immunoglobulin g and m in sera from pregnant and non-pregnant lepromatous leprosy patients. | | 1980 | 7007768 |
| hla-linked control of susceptibility to tuberculoid leprosy and association with hla-dr types. | in an attempt to confirm an hla-linked effect on the course of mycobacterium leprae infection observed in families from surinam (south america), we conducted a similar family study in an endemic area in india. we observed a significant (p less than .05) excess of identical hla-glo haplotypes only from healthy parents among siblings affected with tuberculoid leprosy. compared with healthy controls, unrelated patients with tuberculoid leprosy (n = 15) showed a significant heterogeneity at the hla- ... | 1980 | 7008255 |
| foot pad enlargement as a measure of induced immunity to mycobacterium leprae. | foot pad enlargement (fpe) has been used as a measure of induced immunity to m. leprae, fpe peaked at 2-3 days, but it sometimes persisted for 4 weeks or more. both as the inducing and eliciting antigen, heat-killed m. leprae were effective, and the optimum dose was about 1 x 10(7) bacilli. higher doses were associated with flattening of the dose-response curve. disrupted bacilli were not effective in immunizing mice, but they elicited fpe responses in mice immunized with intact bacilli. cord fa ... | 1980 | 7009447 |
| evidence for species-specific lipid antigens in mycobacterium leprae. | a partially purified lipid fraction from mycobacterium leprae yielded distinct lines of precipitation with antisera from two lepromatus patients and from an infected armadillo. there was no reaction to the sera from patients with tuberculosis or a m. avium infection or to the serum from a normal armadillo. the activity in the lipid fraction was unaffected by mild alkali, and upon hydrolysis the fraction yielded 6-deoxyhexoses. this information suggests that the lipid antigens of m. leprae may be ... | 1980 | 7009448 |
| an improved embedding method for electron microscopy of lepromata. | spurr's resin mixture has been found to be the embedding material most suitable for electron microscopic studies of lepromata at present. like methacrylate, it can penetrate well into foamy structures and the leprosy bacilli within foamy structures. on the other hand, like epon 812, it is stable against the electron beam and can preserve ultrafine structures. additionally, we have found that the use of dimethylformamide or dimethylsulfoxide instead of propylene oxide as substituter improves the ... | 1980 | 7009449 |
| thoughts on the immunology of leprosy. | | 1980 | 7009450 |
| identification problems of strain 0122. | | 1980 | 7009452 |
| genetics and leprosy. | | 1980 | 7009982 |
| multivariate analysis of the activity of rifamycins against rapidly growing mycobacteria, to define an in vitro screening model for their activity against mycobacterium leprae. | in an attempt to select a group of rapidly growing mycobacteria which could serve as a model for the in vitro screening of rifamycin derivatives for their activity against mycobacterium leprae, the mic values of 237 strains of rapidly growing mycobacteria were determined for 4 reference rifamycins with known activity against m. leprae and 19 rifamycins with unknown activity against m. leprae. study of the results by a multivariate statistical technique, the principal components analysis, defined ... | 1980 | 7011330 |
| attempt to induce immune-tolerance to m. leprae in mice. | | 1981 | 7012440 |
| pathologic changes in striated muscles in leprosy. | eighteen muscle biopsies from male adult patients of lepromatous and borderline lepromatous leprosy were studied. bi in the biopsies ranged from 1 + to 3+. there was conspicuous absence of granuloma except for a collection of foamy histiocytes in intermysial region and on two occasions in the intermysial nerve endings in ll patients. at places solid staining bacilli were seen in normal looking muscles. none of the biopsies showed loss of muscle striations, fatty change, sarcolemmal changes, fibr ... | 1981 | 7012441 |
| bcg induced immunity to mycobacterium leprae in mice. | one to three bcg vaccinations were given to various groups of mice--25 to 2 days preceding challenge with myco. leprae. harvesting of myco. leprae was done every month upto a period of 7 months. lymph node enlargement was taken as an indicator of successful vaccination. an increasing degree of protection was noted in bcg immunised mice as observed by a reduced number of bacilli harvested. protection afforded was proportional to the number of doses of bcg given. | 1981 | 7012442 |
| towards a vaccine against leprosy. | | 1981 | 7012444 |
| the immunogenicity of killed mycobacteria. | | 1980 | 7012498 |
| preliminary immunological studies in search of correlates of protective immunity carried out on some iranian leprosy patients and their families. | | 1980 | 7012499 |
| dapsone-resistant leprosy in a population of bamako (mali). | | 1980 | 7012500 |
| assaying dapsone in mouse diets. | | 1980 | 7012501 |
| quantitative study of the separation of mycobacterium leprae from armadillo tissue. | | 1980 | 7012502 |
| does the suppression of the immune response in pre-clinical lepromatous leprosy affect both cell-mediated and humoral responses in mycobacterium leprae antigens? | | 1981 | 7017316 |
| activity of four clofazimine analogues against mycobacterium leprae. | | 1981 | 7017318 |
| the mononuclear cell series in leprosy: an ultrastructural report. | | 1981 | 7017319 |
| selective loss of mycobacterium leprae responsiveness of circulating lymphocytes in primary neuritic leprosy. | | 1981 | 7017320 |
| combined chemotherapy against mycobacterium leprae in the mouse. | | 1980 | 7018423 |
| adenosine triphosphate content in mycobacterium leprae. a brief communication. | | 1981 | 7019109 |
| the 1980 journal--a perspective in leprosy. | | 1981 | 7019112 |
| identification of mycobacterium leprae: use of wall-bound mycolic acids. | a simple method for extraction and analysis of wall-bound mycolic acids from small samples of mycobacteria is described. separation of mycolic acid classes according to their functional groups by thin-layer chromatography showed a difference between mycobacterium leprae and a number of strains of acid-fast bacilli cultured from leprosy biopsies in vitro. this technique is proposed as a convenient preliminary test in the identification of possible cultures of m. leprae. | 1980 | 7019389 |
| [lipidic constituents of "mycobacterium leprae" isolated from experimentally infected armadillo (author's transl)]. | mycobacterium leprae (obtained from experimentally infected armadillo) was submitted to saponification. the liposoluble part was methylated and fractionated by chromatographic methods. each fraction was studied by gas-liquid chromatography. cholesterol (from the infected host) and the main fatty acids were identified. mycolic acids were isolated, and their structures determined, using mass spectrometry. these structures are useful to make a comparison of m. leprae with some other mycobacteria. s ... | 1981 | 7020522 |
| paracrystalline inclusions in mycobacterium leprae. | the occurrence of paracrystalline inclusions of mycobacterium leprae infected with the mycobacteriophage d29 or treated with mitomycin c was reported before [5, 6]. in pursuing these studies we have now documented by electron micrography a number of paracrystals we thought sufficient to further describe these inclusions, and to show that they appeared to be formed in association with the intracellular membranous structures of the leprosy bacilli. | 1981 | 7020523 |
| leprosy without mycobacterium leprae. | | 1980 | 7021515 |
| superoxide dismutase, peroxidatic activity and catalase in mycobacterium leprae purified from armadillo liver. | superoxide dismutase has been identified and peroxidatic activity demonstrated in mycobacterium leprae. the superoxide dismutase, shown indirectly to be a manganese-containing enzyme, was present at low activity in the cell-free extract. peroxidatic activity was detected in a haemoprotein on polyacrylamide gels, but quantitative assay was not possible. catalase, although present in a cell-free extract, appeared to be a host-derived enzyme, thus emphasizing the importance of establishing the auth ... | 1980 | 7021767 |
| incorporation of 3h-thymidine in mycobacterium leprae within differentiated human macrophages. | the factors influencing the incorporation of 3h-thymidine (3h-tdr) in the dna of mycobacterium leprae within macrophages derived from human blood have been evaluated. fifty strains of m. leprae derived from skin nodules of patients with lepromatous leprosy were studied for their ability to incorporate 3h-tdr. control macrophages of the same donor maintained alone, or with autoclaved m. leprae, showed low levels of baseline 3h-tdr incorporation. during a 15-day period of pulsing, 27 of the m. lep ... | 1981 | 7021836 |
| the uptake of 3h-thymidine in mycobacterium leprae inoculated mouse macrophage cultures as a rapid indicator of bacillary viability. factors influencing the specificity of the in vitro assay. | mouse peritoneal macrophages derived from balb/c were used as host cells for 17 human-derived m. leprae strains. simultaneous pulsing with 3h-thymidine (3h-tdr) showed uptake of the radiolabel in 58.8% of the bacilli over a 14 day period. preliminary data of three m. leprae strains indicate that the 3h-tdr is preferentially incorporated into the mycobacterial dna and not into the mammalian host cell dna. this in vitro assay provides a rapid assessment of m. leprae viability. the factors influenc ... | 1981 | 7024148 |
| a novel phenolic glycolipid from mycobacterium leprae possibly involved in immunogenicity and pathogenicity. | a phenolic glycolipid was obtained in high amounts (2% of dry weight) from mycobacterium leprae isolated from infected armadillo liver. infrared and nuclear magnetic resonance spectroscopy showed that it is closely related to "mycoside a" from mycobacterium kansasii and is therefore a glycosylphenolic phthiocerol diester. the crucial difference between the two products is in the composition of the attached trisaccharide. gas-liquid chromatography-mass spectroscopy showed that the product from m. ... | 1981 | 7024248 |
| a report on the biochemical analysis of mycobacterium w. | a mycobacterial strain known as mycobacterial strain w was analysed for its growth characteristics and biochemical traits. this strain was found to be a rapid grower, with luxurient growth on lowenstein-jensen medium, dubos agar, middlebrook's agar and sauton's medium. colonies were smooth, convex and nonpigmented. some of the colonies which appeared rough were similar to smooth colonies at least in biochemical characteristics. this organism was tolerant to wide range of temperatures and to chem ... | 1981 | 7024633 |
| discharge of m. leprae in milk of leprosy patients. | a quantitative estimation of discharge of m. leprae in the milk of 39 leprosy female patients has been made. twelve of the 39 patients (10 ll/bl and 1 each of t/bt and bb) showed bacilli in their milk. only one of these patients was on treatment. afb count in 10 ml of milk was found to range from 4.3 x 10(4) to 4.3 x 10(5). significance of discharge of such a large number of bacilli in breast milk is discussed. | 1981 | 7024634 |
| fourth annual report of the undp/world bank/who special programme for research and training in tropical diseases. | | 1981 | 7024635 |
| the immunology of leprosy: speculations on the leprosy spectrum. | leprosy is a chronic infectious disease caused by mycobacterium leprae. the disease presents a wide spectrum of clinical manifestations, ranging from lepromatous to tuberculoid leprosy; each form may be punctuated by episodes of acute exacerbation, called reactional states. these polar forms and reactional states appear to be determined by immunologic interactions between the host and the microorganism. this review describes the different measurable parameters that permit the classification of a ... | 1981 | 7025147 |
| drug resistance in leprosy. | | 1980 | 7026426 |
| studies of lysosomal enzymes in macrophages. iii. lysosomal enzyme activities in cultured macrophages infected with some species of mycobacteria. | | 1981 | 7028685 |
| [difference in infectivity of mycobacterium leprae from patients in immunosuppressed mice (author's transl)]. | | 1981 | 7028712 |
| [problems in designing a protocol for a leprosy vaccine trial. (author's transl)]. | investigations carried out by the scientific working group on immunology of leprosy (immlep) have led to the planning of field trials of an experimental vaccine preparation to be carried out in few years' time. the objectives of these trials have been defined as follows: 1) the main objective should be the assessment of the protective effect of the vaccine against leprosy, its various forms and more particularly the lepromatous form. 2) another important objective will be to determine the value ... | 1981 | 7029677 |
| [studies on the lipids of "mycobacterium gordonae" in comparison with those of "m. leprae" and of some other scotochromogenic mycobacteria (author's transl)]. | the mycolic acids were isolated from mycobacterium gordonae (strain atcc 14470), and purified by thin layer chromatography. three species were studied by mass spectrometry. the analogy between m. gordonae and m. leprae, based on the lack of tuberculostearic acid, was supported by the comparison of the structures of their mycolic acids. succinct analyses of the lipids of three other scotochromogenic strains of mycobacteria, using thin layer chromatography and gas-liquid chromatography, were perfo ... | 1981 | 7030172 |
| the mycobacterium smegmatis peroxidase, cross-reacting antigen with mycobacterium leprae. | | 1981 | 7030274 |
| potential anti-leprosy vaccine from killed icrc bacilli--a clinicopathological study. | | 1981 | 7030942 |
| the effect of body temperature and cell-mediated immunity on the growth of mycobacterium marinum and mycobacterium leprae in mice. | evidence is presented that the high susceptibility of armadillos to infection with mycobacterium leprae cannot be explained solely in terms of body temperature because mutant mice maintained with a body temperature similar to that of armadillos do not become heavily infected with m. leprae. the depression of cell-mediated immunity accompanying the low body temperature is not sufficient to produce an overwhelming infection. the results obtained with m. marinum suggest that whereas lack of cell-me ... | 1981 | 7031255 |
| a brief review of experiences with short-term clinical trials monitored by mouse-foot-pad-inoculation. | | 1981 | 7031389 |
| pharmacologically-active mediators of hypersensitivity reactions in the blood of lepromatous patients with erythema nodosum leprosum. | | 1981 | 7031390 |
| release of soluble factors from lymph nodes containing mycobacterial granulomas and their effect on fibroblast function in vitro. | | 1981 | 7032710 |
| the effect of delayed addition of antigen and 'e' rosetting on the proliferative response to mycobacterial antigens of peripheral blood lymphocytes from normal individuals or from patients with tuberculosis or leprosy. | some suppressor cells are reported to lose their activity when precultured without stimulus in vitro. we have investigated the role of such suppressors in responsiveness to mycobacterial antigens of peripheral blood mononuclear cells (pbmnc) from patients with leprosy or tuberculosis, or from normal donors. delayed addition of mycobacterial antigens (mycobacterium leprae, mycobacterium vaccae and mycobacterium tuberculosis), but not of a fungal antigen (candida albicans) caused enhanced response ... | 1981 | 7033116 |
| relationship between t-cell population in neonatally thymectomized lewis rats and susceptibility to infection with mycobacterium leprae. | the neonatally thymectomized lewis rat (ntlr) is highly susceptible to infection with m. leprae. however, a significant percentage of ntlr respond to infection with m. leprae in much the same way as do intact rats, yet show no evidence of residual thymus. to determine whether there was a correlation between the number of remaining t-cells and susceptibility to infection with m. leprae, a direct fluorescent antibody test was performed using a highly specific, absorbed antithymocyte globulin label ... | 1981 | 7033149 |
| experimental mycobacterial granulomas in guinea pig lymph nodes: ultrastructural observations. | a systematic ultrastructural study has been performed of the mononuclear phagocytes in granulomas induced by different types of mycobacteria, e.g. live bcg (pasteur), irradiated m. leprae and irradiated bcg (pasteur) in guinea pig lymph nodes. live bcg (pasteur) induces a granuloma which peaks at 2-3 weeks and by light microscopy, a large number of the infiltrating cells have the appearance of epithelioid cells. ultrastructurally a large proportion of these cells have a distinct appearance. they ... | 1981 | 7033491 |
| [studies on m. leprae isolated from the lymph of a lepromatous patient using the freeze-fracture technic]. | | 1981 | 7034060 |
| induction of delayed-type hypersensitivity to mycobacterium leprae in healthy individuals. | delayed-type hypersensitivity (dth) to a soluble mycobacterium leprae skin test antigen (sml) was successfully induced in healthy volunteers following immunization with 2 x 10(8) killed armadillo-derived m. leprae. no better sensitization was obtained by a mixture of live bcg and killed m. leprae. the relative specificity of the dth reaction to sml has been demonstrated in this study, since little cross-reactivity was observed to ppd, after immunization with bcg ro m, leprae alone, or combined. ... | 1981 | 7035030 |
| failure to induce delayed-type hypersensitivity to mycobacterium leprae in long-term treated lepromatous leprosy patients. | lepromatous leprosy (ll) patients whose bacillary load has decreased to almost undetectable levels by long-term chemotherapy failed to develop delayed-type hypersensitivity (dth) to mycobacterium leprae antigen following immunization with killed armadillo-derived m. leprae. when these ll patients were immunization with killed armadillo-derived m. leprae. when these ll patients were immunized with killed m. leprae in a mixture with live bcg, only dth to purified protein derivative (ppd) was induc ... | 1981 | 7035031 |
| applications of flow cytometry on bacteria: cell cycle kinetics, drug effects, and quantitation of antibody binding. | | 1982 | 7035105 |
| the association of pregnancy and leprosy. ii. pregnancy in dapsone-resistant leprosy. | | 1981 | 7035779 |
| activity of the combination of isoniazid, protionamide and dapsone against mycobacterium leprae and some other mycobacteria. | no additive effect was found at the addition of isoniazid to either dapsone or protionamide against m. leprae and against highly dapsone-sensitive in vitro growing mycobacterium sp. strains. it is concluded that the indication of the administration of isoniazid in leprosy is extremely limited and could only be envisaged as a companion drug in combined therapy of dapsone-resistant multibacillary leprosy in light-skinned people, when aminoglycosides cannot be administered. | 1981 | 7037011 |
| a comparison of the bactericidal activity of a series of rifampicins against mycobacterium leprae. | the bactericidal activity against mycobacterium leprae of six 3-(4-methylpiperazin-1-yl-iminomethyl)rifamycin sv (rifampicin) derivatives was compared with that of rifampicin in a modified proportional bactericidal test, using a smaller number of experimental animals and allowing the administration of a single dose of the drugs. no correlation was found between the in vitro activity of the rifamycin derivatives against m. tuberculosis or rapidly growing mycobacteria in vitro and the activity aga ... | 1982 | 7037013 |
| serology of leprosy. ii. demonstration of specific antibodies to mycobacterium leprae by counter current electrophoresis. | a rapid and simple method for the demonstration of specific antibodies to mycobacterium leprae using counter current electrophoresis (cce) has been described. three types of antigens (sonicate antigen, surface antigen and purified protoplasmic protein antigen) were prepared from purified m. leprae and tested against sera from patients with different types of leprosy, and their healthy household contacts, by cce. antibodies specific to m. leprae were demonstrated by cce after absorption of sera w ... | 1981 | 7038297 |
| skin test response to m. leprae precursor coccoid organisms in a high leprosy endemic area as compared to lepromin. | the paper describes the results on several hundred skin tests on humans using antigens from leprosy derived non-acid fast coccoid organisms, and a mycobacterium that grew out of one of the coccoid organisms. early skin test response to the cytoplasmic fraction of one of the leprosy derived, non-acid fast, coccoid organism was very highly correlated with standard lepromin. | 1981 | 7038299 |
| inhibition of foot pad multiplication of m. leprae in mice vaccinated with leproma derived m. leprae precursor coccoid organism and passive transfer of dth response to normal mice through infusion of splenic cells of such vaccinated mice. | | 1981 | 7038300 |
| abstract of papers presented at the international symposium on mycobacterium leprae and other atypical mycobacteria. | | 1981 | 7038301 |
| [terminal innervation in 3 cases of leprous neuropathy (author's transl)]. | three cases of leprosy are reported. in all patients, the diagnosis was made by finding hansen bacilli in skin lesions, nasal secretions and ears, and the immunological typing of leprosy was established. conduction velocity of motor and sensory nerves was measured and muscle biopsies were obtained. histochemical typing of muscle fibers and measurement of the terminal innervation ratio of motor axon vitally stained with methylene blue were performed. a subclinical involvement of peripheral motor ... | 1981 | 7038820 |
| iga and igm antibodies against mycobacterium leprae in cord sera and in patients with leprosy: an indicator of intrauterine infection in leprosy. | a solid-phase radioimmunoassay was developed for demonstration and quantification of iga and igm anti-m. leprae antibodies. iga and igm anti-m. leprae antibodies were demonstrated in a lepromatous serum pool, in various amounts in individual patients with lepromatous leprosy, and in lower concentration in tuberculoid leprosy and non-leprosy controls. iga and igm anti-m. leprae antibodies were demonstrated in cord sera from babies of mothers with leprosy. the reliability of fetal iga and igm anti ... | 1981 | 7038849 |
| thymus-dependent lymphocytes in leprosy. ii. effect of chemotherapy on t-lymphocyte subpopulations. | the basis of the immunological unresponsiveness seen in leprosy patients is unknown. untreated lepromatous leprosy patients display an unspecific cellular anergy which disappears with treatment, leaving an anergy specific for mycobacterium leprae. these patients suffer from a complication, erythema nodosum leprosum, characterized by a recurrent eruption of tender skin nodules disappearing in 2 to 3 days. these nodules show a histological picture reminiscent of an arthus reaction. erythema nodosu ... | 1982 | 7040440 |
| leprosy. | | 1982 | 7040711 |
| mycobacterium leprae and the host response. | | 1981 | 7040872 |
| the lymphocyte transformation test in leprosy with special reference to its use in epidemiology. | | 1981 | 7040878 |
| in vitro lymphocyte stimulation using a purified antigen of mycobacterium leprae and tuberculin ppd. | | 1981 | 7040879 |
| subclinical infection in leprosy--its detection and control by fluorescent leprosy antibody absorption (fla-abs) test. | | 1981 | 7040880 |
| radioimmunoassay and other serologic tests and their application in epidemiological work. | | 1981 | 7040881 |
| leprosy--the source of infection and its mode of transmission. | | 1981 | 7040883 |
| occurrence of non-cultivable acid-fast bacilli in the environment and their relationship to m. leprae. | | 1981 | 7040884 |
| reversion of the downhill course of active lepromatous leprosy by repeated transfusions of fresh blood, donated by healthy but lepromin positive patients. | earlier studies of immunologic reconstitution therapy of active lepromatous leprosy patients by repeated infusions of viable allogeneic blood lymphocytes indicated a beneficial effect. in order to avoid cell separation and its associated risks, we attempted to transfer immunity passively into seven seriously ill lepromatous patients by repeated transfusions of fresh blood donated by healthy, but tuberculin and lepromin positive, subjects. the results showed clinical improvement in some cases wit ... | 1982 | 7041343 |
| [pathogenic mycobacteria]. | it is well established that mycobacteria other than tubercle bacilli and m. leprae are important human pathogens. at least 15 species comprise all the presently known mycobacterial pathogens of man. eleven species are potential pathogens because disease production often dependent on special factors of the human host. the comments include some characterization of the mycobacteria, especially of the potential pathogenic species. a summary of important nontuberculous mycobacterial diseases of man i ... | 1982 | 7041441 |