| activation of the e2f transcription factor in adenovirus-infected cells involves e1a-dependent stimulation of dna-binding activity and induction of cooperative binding mediated by an e4 gene product. | previous experiments have demonstrated that the dna-binding activity of the e2f transcription factor is increased upon adenovirus infection and that both the e1a and e4 genes are required for activation. in this study, we demonstrated that this enhanced binding of e2f to the e2 promoter is the result of two events. (i) there is stimulation of the dna-binding activity of the e2f factor; this stimulation is e1a dependent but independent of e4. (ii) there is also induction of a stabilized interacti ... | 1990 | 2139893 |
| dna damage and prophage induction and toxicity of nitrofurantoin in escherichia coli and vibrio cholerae cells. | repair-deficient and repair-proficient strains of e. coli k12 were sensitive to nitrofurantoin treatment to varying degrees with the double mutant strain (uvra 6, reca 13) being most sensitive. ultraviolet absorption data and thermal chromatography through a hydroxyapatite column revealed that nitrofurantoin treatment of v. cholerae strain ogawa 154 produced a maximal amount of 55% reversibly bihelical dna at a nitrofurantoin dose of 120 micrograms/ml/h, which indicated the formation of inter-st ... | 1990 | 2139919 |
| contribution of u1 snrna structural domains to u1 snrnp function. | | 1990 | 2141898 |
| influence of chronic alterations of salt intake and aging on the kinetic of red cell na+ and k+ transport in sprague-dawley rats. | the kinetics of na+ and k+ (rb)+ transport mediated by the na(+)-k+ pump and na(+)-k+ cotransport system (assessed as a function of rb+o and na+i) as well as the magnitude of cation leaks were determined in red cells of young male rats subjected to chronic salt deprivation or salt loading (0.1% and 8% nacl diet). these salt intake alterations induced moderate kinetic changes of the na(+)-k+ pump which did not result in significant changes of ouabain-sensitive (os) rb+ uptake or na+ net extrusion ... | 1990 | 2142786 |
| stimulation of a human erythrocyte membrane atpase by glutathione conjugates. | an atp-dependent transport process for s-(2,4-dinitrophenyl) glutathione (dnp-sg) mediated by a novel atpase designated as dnp-sg atpase has been demonstrated in human erythrocytes (labelle et al., febs lett. 228, 53-51, 1988). in order to investigate whether the dnp-sg atpase system represents a generalized mechanism for the transport of xenobiotic conjugates of glutathione (gsh), stimulation of this atpase by different gsh conjugates was studied in membrane vesicles prepared from human erythro ... | 1990 | 2143605 |
| [comparative study of cholera phages isolated from the water of open reservoirs and lysogenic strains of vibrio el-tor]. | | 1990 | 2144501 |
| iron-binding compounds and related outer membrane proteins in vibrio cholerae non-o1 strains from aquatic environments. | a total of 156 strains of vibrio cholerae non-o1 from aquatic origins were examined for the presence of iron uptake mechanisms and compared with o1 strains and other vibrio species. all non-o1 strains were able to grow in iron-limiting conditions, with mics of ethylenediaminedi (o-hydroxyphenylacetic acid) ranging from 20 microm to 2 mm. the production of siderophores was demonstrated by growth in chrome azurol s agar and cross-feeding assays. all strains produced phenolate-type compounds, as as ... | 1990 | 2144956 |
| [the epidemiological importance of vibrio cholerae isolated from different ecological systems]. | the analysis of the data on the isolation of v. cholerae from different ecological systems indicates that v. eltor do not constantly inhibit the rivers and sea at the territory under control. hemolytically active v. cholerae without the vct gene, found to be faintly virulent and avirulent when studied on suckling rabbits used as a model and when evaluated by the complex method, show no tendency towards epidemic spread in the presence of conditions for the realization of the transmission of vibri ... | 1990 | 2146847 |
| preparation and characterization of anti-paroxetine antibodies. | 6-nitroparoxetine was synthesized and reduced to 6-aminoparoxetine. after coupling to glutaraldehyde at the 6-position and to bovine serum albumin, the resulting schiff's base was further reduced into an amino-derivative which served as the antigen. anti-paroxetine antibodies were raised against this antigen in rabbits and the anti-paroxetine igg purified by protein a affinity chromatography. the anti-paroxetine igg demonstrated high specificity towards paroxetine and 6-nitroparoxetine without s ... | 1990 | 2147049 |
| [the epidemiological aspects of the importation of cholera into the territory of the uzbek ssr in recent years]. | | 1990 | 2150142 |
| [sensitivity of enteropathogenic bacteria to furazolidone]. | the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ... | 1990 | 2152485 |
| identification of an iron-regulated virulence determinant in vibrio cholerae, using tnphoa mutagenesis. | several virulence determinants of bacteria are regulated by the concentration of iron in the medium, with increased expression occurring under low-iron conditions. iron-regulated virulence factors have not been previously described in vibrio cholerae. we used the transposon vector tn5 is50l::phoa (tnphoa) to obtain insertion mutations in iron-regulated genes of v. cholerae 0395. one strain, carrying an insertion mutation in iron-regulated gene irga, had reduced virulence in an animal model and h ... | 1990 | 2152889 |
| interaction of cholera toxin with cloned human goblet cells in monolayer culture. | exposure of the intestinal mucosa to vibrio cholerae enterotoxin (ct) results in mucus secretion from intestinal goblet cells. on the other hand, there is evidence that elevation of intracellular adenosine 3',5'-cyclic monophosphate levels is not sufficient to induce rapid mucin secretion. to determine whether ct has direct effects on human goblet cells and whether ct alone can elicit rapid exocytosis of apical mucin granules, purified ct was applied to monolayer cultures of well-differentiated ... | 1990 | 2154122 |
| comparative studies of azithromycin in skin and soft-tissue infections and sexually transmitted infections by neisseria and chlamydia species. | two open, randomized, single centre studies have investigated the efficacy and safety of azithromycin (cp-62,993) in the treatment of infections by azithromycin-sensitive pathogens: (a) acute bacterial infections of skin or soft tissue (compared with erythromycin; n = 82); and (b) urethritis and/or cervicitis caused by neisseria gonorrhoeae and/or chlamydia trachomatis (compared with doxycycline; n = 108). in study a, azithromycin was administered to 42 patients for five days at a dosage of 250 ... | 1990 | 2154430 |
| future vaccines against enteric pathogens. | a small number of bacterial agents, including enterotoxigenic escherichia coli (etec), enteropathogenic e. coli (epec), shigella and vibrio cholerae 01 and one virus, rotavirus, combine to cause a major proportion of the diarrheal illness of public health importance worldwide. salmonella typhi is by far the major cause of enteric fever. attempts to develop safe, practical, and effective vaccines against these agents are under way. examples of vaccine candidates include live oral vaccines against ... | 1990 | 2155260 |
| characterization of the hlyb gene and its role in the production of the el tor haemolysin of vibrio cholerae o1. | el tor strains of vibrio cholerae are capable of producing a haemolysin which they actively secrete into the growth medium. this requires translation to produce the protein at the surface of the cytoplasmic membrane and translocation across this membrane, the periplasmic space and the outer membrane. the mechanism by which this occurs is poorly understood. in addition to the structural gene for the haemolysin (hlya), we have cloned a second adjacent gene, hlyb. by site-directed mutagenesis, spec ... | 1990 | 2162464 |
| induction of ornithine decarboxylase mrna in cerebral cortex in response to lesion of nucleus basalis. | | 1990 | 2164982 |
| subunit analysis of bovine cytochrome c oxidase by reverse phase high performance liquid chromatography. | bovine cytochrome c oxidase subunits were separated by reverse phase high performance liquid chromatography using a c4 column eluted with water and an acetonitrile gradient, both containing 0.1% trifluoroacetic acid. subunits i and iii precipitated in this solvent and could not be analyzed; the remaining eleven subunits were dissociated, denatured, soluble and could be resolved by elution from the column. the protein subunit eluting in each chromatographic peak was identified by a combination of ... | 1990 | 2168156 |
| kinetics of the reaction of prostaglandin h synthase compound ii with ascorbic acid. | the reduction of prostaglandin h synthase compound ii by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 m phosphate buffer (ph 8.0) at 4.0 +/- 0.5 degrees c, by rapid scan spectrometry and transient state kinetics. a saturation effect and nonzero intercept were observed in the plot of pseudo-first-order rate constant versus ascorbic acid concentration. the saturation behavior suggests formation of a complex between prostaglandin h synthase compound ii and ascorbic aci ... | 1990 | 2168160 |
| presence of human papillomavirus type-6-related sequences in inverted nasal papillomas. | twenty dna samples obtained from seven cases of inverted papillomas, eight cases of nasal polyps and five cases of chronic sinusitis were investigated by southern blot hybridization for the possible presence of sequences homologous to human papillomavirus (hpv) types 6, 11, 16 and 18. hpv type-6-related dna was identified in one of the seven inverted papillomas. the restriction endonuclease cleavage patterns showed that this latter dna is a new subtype of hpv type 6 dna. in the other six papillo ... | 1990 | 2168185 |
| vectorial electron and proton transfer steps in the cytochrome bc1 complex. | | 1990 | 2168207 |
| a progesterone-modulated, low-molecular-weight protein from the uterus of the sheep is associated with crystalline inclusion bodies in uterine epithelium and embryonic trophectoderm. | a protein of mr 14,000 (14k protein) has been identified in the medium after culturing sheep conceptuses in vitro. however, it was also a component of uterine flushes of nonpregnant ewes during the luteal phase of the estrous cycle. the 14k protein, which consists of several isoforms, was purified from conceptus culture medium by a simple three-step procedure, involving ammonium sulfate precipitation, high-performance anion-exchange chromatography, and gel filtration on sephacryl s-200. western ... | 1990 | 2168219 |
| cytomegalovirus nucleic acids in allografted hearts. | | 1990 | 2168339 |
| enhancement by calcium of the detection of cytomegalovirus in cells treated with dexamethasone and dimethyl sulfoxide. | mrc-5 cultures treated with dexamethasone, dimethyl sulfoxide, and calcium were compared with untreated cultures and cultures treated with dexamethasone and dimethyl sulfoxide for the detection of human cytomegalovirus by the shell vial method. the addition of calcium resulted in significantly increased growth of human cytomegalovirus ad169. protein studies and cell counts showed renewed cell proliferation in the treated cultures. when low-titer clinical specimens were retested, 27.5% of all pos ... | 1990 | 2168437 |
| accuracy and precision of computer-simulated tissue temperatures in individual human intracranial tumours treated with interstitial hyperthermia. | accurate knowledge of tissue temperature is necessary for effective delivery of clinical hyperthermia in the treatment of malignant tumours. this report compares computer-predicted versus measured intratumoral temperatures in 11 human subjects with intracranial tumours, treated with a conceptually simple 'conductive' interstitial hyperthermia system. interstitial hyperthermia was achieved by the use of parallel arrays of implanted, electrically heated catheters. the tissue was warmed by thermal ... | 1990 | 2168460 |
| urinary bladder blood flow. i. comparison of clearance of locally injected 99mtechnetium pertechnate and radioactive microsphere technique in dogs. | the blood flow of the dog urinary bladder measured by radioactive microsphere technique was compared to the clearance of locally injected 99mtechnetium pertechnate (99mtc) in the bladder wall. in semilogarithmic plots the 99mtc washout curves showed a multiexponential course. from the initial slopes (median 5.7 min) the bladder blood flow was calculated to be only 30-62% of the results obtained from the radioactive microsphere technique (blood flow in the muscular layer 21.7-44.8 ml/100 g/min). ... | 1990 | 2168600 |
| protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves. | formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ... | 1990 | 2168606 |
| increase of the anion and proton permeability of saccharomyces carlsbergensis plasmalemma by n-alcohols as a possible cause of its de-energization. | the effect of n-alcohols on atp-dependent generation of delta ph and em across the plasma membrane vesicles of the yeast saccharomyces carlsbergensis was investigated. the alcohols were shown to collapse delta ph and em in the order c2 less than c3 less than c4 less than c5 less than or equal to c6 greater than or equal to c7 greater than c8 greater than c11, the dissipation of em being more pronounced. inhibition of the plasmalemma h(+)-atpase was insignificant; at low alcohol concentrations it ... | 1990 | 2168610 |
| mechanisms underlying the stimulation of pth release by gppnhp in permeabilized bovine parathyroid cells. | we examined changes in camp and inositol phosphate metabolism to assess the contribution of the guanine nucleotide regulatory (g) protein(s) regulating adenylate cyclase and phospholipase c in mediating the stimulatory effects of gppnhp on pth release from permeabilized bovine parathyroid cells. to examine the role of gs, the g protein stimulating adenylate cyclase, and camp on pth release, permeabilized cells were incubated with either gppnhp or isoproterenol, and the effects of these agents on ... | 1990 | 2168660 |
| effect of superoxide dismutase in cats with cold-induced edema. | we evaluated the potential role of sod, an oxygen free radical scavenger, as a probe to cover the trigger period of injury and the prolonged period of development of edema in vasogenic brain edema. vasogenic brain edema was produced in 34 cats by a standardized cortical freezing lesion. brain edema and the disruption in the bbb were assessed by sg measurement and spread of evans blue by planimetry. detection of superoxide radicals was studied by topical application of nbt within the cranial wind ... | 1990 | 2168663 |
| proton-nuclear magnetic resonance relaxation times in brain edema. | proton relaxation times of protein solutions, bovine brain, and edematous feline brain tissue were studied as a function of water concentration, protein concentration, and temperature. in accordance with the fast proton exchange model for relaxation, a linear relation could be established between r1 and the inverse of the weight fraction of tissue water. this relation also applied to r2 of gray matter and of protein solutions. no straightforward relation with water content was found for r2 of wh ... | 1990 | 2168666 |
| phosphonylmethyl ethers of acyclic nucleoside analogues: inhibitors of hsv-1 induced ribonucleotide reductase. | diphosphates of n-(s)-(3-hydroxy-2-phosphonylmethoxypropyl) and n-(2-phosphonylmethoxyethyl) derivatives of purine and pyrimidine heterocyclic bases inhibit hsv-1 encoded ribonucleotide reductase. of the compounds studied, the most efficient inhibitors of cdp reduction (at 5.1 mumols.l-1) by the hsv-1-encoded enzyme are hpmpapp (ic50 = 0.9 mumols.l-1) and pmeapp (ic50 = 8 mumol.l-1). pmeapp does not inhibit the enzyme isolated from the mutant hsv-1 kos strain pmear which is resistant to pmea at ... | 1990 | 2168691 |
| antiinflammatory properties of the new antirheumatic agent 4-acetylaminophenylacetic acid. | the antiinflammatory activity of 4-acetylaminophenylacetic acid (ms-932) was investigated. ms-932 did not suppress the acute inflammation of carrageenin-induced paw edema in rats or of primary swelling in adjuvant arthritic rats. however, prophylactic treatment with ms-932 inhibited secondary inflammation in adjuvant arthritic rats. ms-932 also restored to normal the weight of the spleen and the serum albumin/globulin ratio of adjuvant arthritic rats. in addition to its prophylactic effect, ms-9 ... | 1990 | 2168705 |
| establishment of ddd/1-mtv-2/mtv-2, nu/nu and ddd/1-mtv-2/mtv-2, nu/+ mice: preliminary characterization in relation to mtv antigen expression and mammary tumorigenesis. | to investigate the effects of the t-cell deprivation on viral mammary tumorigenesis, two double congenic mouse strains of the ddd genetic background, ddd/1-mtv-2/mtv-2, nu/nu and ddd/1-mtv-2/mtv-2, nu/+, were produced by the cross between ddd/1-mtv-2/mtv-2 (ddd-mtv-2) and ddd/1-nu/nu mice, followed by repeated intercross breedings. expression of the mouse mammary tumor virus (mtv)-gp52 antigen was demonstrated in the mammary glands of mice from 14 days on, in both -nu/nu and -nu/+ females. mamma ... | 1990 | 2168807 |
| mu-opioid receptors mediate the inhibitory effect of opioids on dopamine-sensitive adenylate cyclase in primary cultures of rat neostriatal neurons. | the receptors mediating the inhibition of d1 dopamine receptor-stimulated adenylate cyclase by opioids were examined in primary cultures of rat neostriatal neurons. adenylate cyclase activity was dose-dependently increased by the selective d1 dopamine receptor agonist skf 38393 (ec50 = 0.05 microm). this stimulation was fully antagonized by the selective d1 dopamine receptor antagonist sch 23390 (1 microm). skf 38393 (1 microm)-stimulated adenylate cyclase activity was strongly reduced (by almos ... | 1990 | 2168934 |
| low-energy laser irradiation--a new measure for suppression of arachidonic acid metabolism in the optic nerve. | helium-neon low-energy laser (lel) irradiation, known for its therapeutic effects in arthritis, wound healing, and muscular strain, was applied to eyes of rats that had sustained an optic nerve crush injury. crush injury to the optic nerve resulted in a long-lasting in vitro elevation of prostaglandin e2 (pge2) production (1.8-3.9-fold above control values during the 12 day study period). lel irradiation per se had no effect in vitro on pge2 production by the optic nerve; however, lel irradiatio ... | 1990 | 2168952 |
| ebna size polymorphism can be used to trace epstein-barr virus spread within families. | the epstein-barr virus (ebv)-determined nuclear antigens ebna 1, 2, 3, 4, and 6, regularly expressed in ebv-transformed lymphoblastoid cell lines, vary in size among viral strains. we have used this characteristic to trace the spread of the virus within seven families by using an approach called ebnotyping. among 33 evaluable individuals, 3 were ebv seronegative, and 17 different ebv strains could be isolated from the peripheral blood or throat washes of the remaining 30. all unrelated persons c ... | 1990 | 2168960 |
| pertussis toxin b-subunit-induced ca2(+)-fluxes in jurkat human lymphoma cells: the action of long-term pre-treatment with cholera and pertussis holotoxins. | the exotoxins of bordetella pertussis and vibrio cholera have been used to investigate signal transduction in the human t-cell lymphoma jurkat. stimulation of the cells, leading to an increase in cytoplasmic free calcium, could be achieved by the anti-t-cell receptor complex antibody okt3 and by pertussis holotoxin (ptht), or its b-subunit (ptb), but not by cholera holotoxin (ctht) or its b-subunit (ctb). both holotoxins adp-ribosylated specifically g-proteins in the plasma membrane of intact ce ... | 1990 | 2169284 |
| photoprotective effect of superoxide-scavenging antioxidants against ultraviolet radiation-induced chronic skin damage in the hairless mouse. | albino hairless mice (skh:hr-1) exposed chronically to suberythemal doses of ultraviolet radiation develop visible skin changes, histological alterations, and tumors. topical treatment of mice with solutions of superoxide-scavenging antioxidants (such as alpha-tocopherol, ascorbic acid, propyl gallate and trolox) prior to each uvb radiation exposure reduced significantly the severity of these events. tocopherol esters and ascorbyl palmitate were not as effective as the parent compounds in provid ... | 1990 | 2169296 |
| effects of a novel 21-amino steroid, u74006f, on the rat pituitary-adrenocortical axis. | the 21-amino steroid u74006f is a potent inhibitor of lipid peroxidation and has been shown to affect beneficially the acutely injured central nervous system. therapeutically, it is desirable for this compound to be devoid of steroid side-effects. we have demonstrated a significant (p less than 0.001) inhibition of basal acth secretion from cultured rat pituitary cells during a 24-h incubation at concentrations (10-100 mumols/l) previously demonstrated to inhibit lipid peroxidation in vitro. u74 ... | 1990 | 2169520 |
| osteoblasts display receptors for and responses to leukemia-inhibitory factor. | specific binding of leukemia-inhibitory factor (lif) to osteoblasts, but not multinucleated osteoclasts, was demonstrated by receptor autoradiography by using cells isolated from newborn rat long bones. the clonal rat osteogenic sarcoma cells, umr 106-06, which have several phenotypic properties of osteoblasts, expressed 300 lif receptors per cell, with an apparent kd of 60 pm. treatment of calvarial osteoblasts or umr 106-01 cells with lif resulted in a dose-dependent inhibition of plasminogen ... | 1990 | 2170427 |
| evidence for a role of the na+/h+ exchanger in the colony-stimulating-factor-induced ornithine decarboxylase activity and proliferation of the human cell line m-07e. | the subclone m-07e, derived from the interleukin-3 (il-3)-responsive human myeloid cell line m-07, is strictly dependent on either il-3 or granulocyte-macrophage-colony-stimulating factor (gm-csf) for its growth and survival. this cell line may be regarded as a candidate model to investigate the poorly understood events triggered by growth factors binding to human hemopoietic cells. both il-3 and gm-csf induce in m-07e cells an increase of ornithine decarboxylase (odc) activity, which reaches it ... | 1990 | 2170428 |
| cytomegalovirus-induced pneumonitis and myocarditis in newborn mice. a model for perinatal human cytomegalovirus infection. | genetically determined resistance to the lethal effects of infection with murine cytomegalovirus (mcmv) has been reported previously in adult and newborn mice. we examined the pathogenesis of mcmv infection in resistant (cba, h-2k) and susceptible (balb/c, h-2d) mice infected intraperitoneally on the day of birth. balb/c mice developed a severe interstitial pneumonitis and myocarditis 10 days post-infection. their pulmonary and tissues contained high mcmv titres and large numbers of mcmv-antigen ... | 1990 | 2174234 |
| a nontraditional role for water in the cytochrome c oxidase reaction. | the passage of electrons through cytochrome c oxidase is directly related to the activity of water. reducing the activity in a system containing reductant, oxygen, and cytochrome oxidase blocks electron transfer between reduced cytochrome a and oxidized cytochrome a3. the extent of the block is directly related to the osmotic pressure of the system, implying that the protein shell of the oxidase acts as a semipermeable membrane that excludes osmotic perturbants but not water. it appears that app ... | 1990 | 2174258 |
| two-step processing for activation of the cytolysin/hemolysin of vibrio cholerae o1 biotype el tor: nucleotide sequence of the structural gene (hlya) and characterization of the processed products. | vibrio cholerae o1 biotype el tor produces and secretes a 65-kda cytolysin/hemolysin into the culture medium. we cloned the structural gene (hlya) for the cytolysin from the total dna of a v. cholerae o1 el tor strain, n86. nucleotide sequence analysis of hlya revealed an open reading frame consisting of 2,223 bp which can code for a protein of 741 amino acids with a molecular weight of 81,961. consistent with this, a 79-kda protein was identified as the product of hlya by maxicell analysis in e ... | 1990 | 2174833 |
| transcriptional regulation by iron of a vibrio cholerae virulence gene and homology of the gene to the escherichia coli fur system. | we have previously described an iron-regulated virulence determinant in vibrio cholerae. strain mbg40, which contains a tnphoa insertion mutation in the iron-regulated gene irga, has reduced virulence in a newborn mouse model and has lost the major 77-kda iron-regulated outer membrane protein. we report here the cloning of the irga'-'phoa gene fusion, the sequencing of the 5'-proximal portion of irga, and the definition of its promoter region by primer extension. the deduced amino acid sequence ... | 1990 | 2174861 |
| a new selective, differential agar medium for isolation of vibrio cholerae o1: pmt (polymyxin-mannose-tellurite) agar. | a new selective and differential agar medium, polymyxin-mannose-tellurite (pmt) agar was devised to differentiate easily colonies of vibrio cholerae o1 from those of v. cholerae non-o1. the differentiation between colonies of the two vibrios is based on mannose-fermentation. colonies of v. cholerae o1 on the agar are agglutinated with o1 antiserum of v. cholerae much more easily than those on thiosulfate-citrate-bile salts-sucrose (tcbs) agar. | 1990 | 2175000 |
| [cholestyramine adsorbs escherichia coli and vibrio cholerae toxins by way of ion exchange mechanism]. | the use of cholestyramine in the treatment of enterocolitis is justified by its ability to fix the lt toxin of vibrio cholerae and etec, the st toxin of etec and the verotoxin of ehec at the ph of intestinal fluid. to elucidate the nature of the ion-exchange mechanism in the binding of the toxins on the resin, we try to eluate the toxins with 0.1 m to 0.9 m nacl solution at ph 7. irrespective of their presence or absence in the different fractions, the biological activity of the toxins was asses ... | 1990 | 2181919 |
| high- and low-density lipoproteins enhance infection of trypanosoma cruzi in vitro. | trypanosoma cruzi exhibits a developmentally regulated neuraminidase activity that is inhibited by high-density lipoprotein (hdl). we report here that the infection of culture cells by t. cruzi trypomastigotes is enhanced by hdl in a dose-dependent manner. the enhanced infection is prevented by vibrio cholerae neuraminidase, an enzyme whose activity is not inhibited by hdl, suggesting that sialic acid is involved in t. cruzi-host interaction. similar enhancement of infection is also produced by ... | 1990 | 2183047 |
| diabetes mellitus, the renin-angiotensin system, and angiotensin-converting enzyme inhibition. | many traditional antihypertensive drugs are linked to various effects that may cause concern in the hypertensive diabetic patient. the favorable tolerability of angiotensin-converting enzyme (ace) inhibitors in essential hypertension makes it likely that they will be well tolerated when used in diabetic patients. data from ongoing studies support the theory that ace inhibitors lower blood pressure to the same extent in hypertensive diabetics as in patients with essential hypertension. ace inhibi ... | 1990 | 2189073 |
| construction of a bivalent oral vaccine for prevention of typhoid fever and cholera diarrhea. | a recombinant plasmid pmm-ctb containing the gene for production of the nontoxic b subunit of vibrio cholera was transferred into a safe, effective and attenuated oral vaccine ty21a strain of salmonella typhi. the resulting ty21a (pmm-ctb) could steadily produce ct-b subunit that was secreted extracellularly and had the same antigenicity as ct-b produced by v. cholera. furthermore, the characteristics of the antigenicity, the persistance in mice and the galactose sensitivity possessed in the str ... | 1990 | 2190570 |
| vibrio cholerae serogroup o1 in northeast thailand. | strains of vibrio cholerae serogroup o1 biotype el tor that are susceptible to mukerjee cholera bacteriophage group iv (s. mukerjee, bull. w.h.o. 28:333-336, 1963) were found. cholera vibrios isolated from epidemics in northeast thailand were characterized, and 57 of 60 strains isolated in 1986 were susceptible to cholera phage iv. however, all 113 strains isolated in 1988 were not susceptible to the phage. all isolates in both epidemics revealed behaviors typical of el tor vibrios, except phage ... | 1990 | 2191009 |
| binding of bacteria to carbohydrates immobilized on beads to demonstrate the presence of cell-associated hemagglutinins in vibrio cholerae. | we describe a phase contrast microscopy method for direct observation of classical and el tor vibrios to agarose beads containing covalently attached l-fucose or d-mannose. binding of the vibrios to l-fucose beads was found to correlate with fucose-sensitive agglutination of human o erythrocytes, while binding of bacteria to beads with d-mannose was consistent with mannose-sensitive agglutination of chicken erythrocytes. furthermore, vibrios expressing both fucose and mannose-sensitive hemagglut ... | 1990 | 2191694 |
| factors influencing semen quality in pigs. | | 1990 | 2192031 |
| no acquisition of metastatic capacity of r1h rhabdomyosarcoma upon transfection with c-ha-ras oncogene. | the effect of the activated c-ha-ras oncogene on invasiveness and formation of spontaneous metastases was studied using the rhabdomyosarcoma r1h of the rat. r1h tumor cells which are able to grow in vitro and produce tumors upon subcutaneous injection in syngeneic wag/rij rats were transfected with the c-ha-ras (ej) oncogene and the neomycin gene for selection. two r1h cell lines harboring and expressing the human c-ha-ras oncogene, one cell line containing the neomycin gene only, and the parent ... | 1990 | 2192992 |
| [inhibition of enteropathogens by clostridium butyricum miyairi 588]. | the inhibitory effect of clostridium butyricum miyairi 588 against various enteropathogens was investigated in mixed cultures. it was observed that c. butyricum m588 inhibited the growth of vibrio cholerae o1, v. cholerae non-o1, aeromonas hydrophila, and shigella flexneri. considering that the interaction between c. butyricum and shigella is especially important because of their proliferation site in the lower intestine, further examinations were carried out on shigella in particular. results w ... | 1990 | 2193065 |
| hepatic apo b-100 lipoproteins and plasma ldl heterogeneity in african green monkeys. | the contribution of hepatic apolipoprotein (apo) b-100 lipoproteins to plasma low-density lipoprotein (ldl) metabolic heterogeneity was examined in african green monkeys. hepatic 3h-labeled very low-density lipoproteins (vldl) (d less than 1.006, where d is density in g/ml) or hepatic 131i-labeled ldl (1.030 less than d less than 1.063) were isolated from perfused livers and injected simultaneously with autologous plasma 125i-ldl into african green monkeys. serial blood samples were taken, and t ... | 1990 | 2193530 |
| direct effect of parathyroid hormone on insulin secretion from pancreatic islets. | indirect evidence indicates that parathyroid hormone (pth) interacts with pancreatic islets and modulates their insulin secretion. this property of pth has been implicated in the genesis of impaired insulin release in chronic renal failure. we examined the direct effect of pth-(1-84) and pth-(1-34) on insulin release using in vitro static incubation and dynamic perifusion of pancreatic islets from normal rats. both moieties of the hormone stimulated in a dose-dependent manner glucose-induced ins ... | 1990 | 2193536 |
| double-blind, randomized, controlled clinical trial of norfloxacin for cholera. | in a double-blind, randomized clinical trial with 78 adults with acute watery diarrhea and severe dehydration, 37 subjects were positive for vibrio cholerae. in conjunction with rehydration therapy, 13 patients received norfloxacin, 12 received trimethoprim-sulfamethoxazole (tmp-smx), and 12 received a placebo. norfloxacin was superior to tmp-smx and to the placebo in reducing stool output, duration of diarrhea, fluid requirements, and vibrio excretion. tmp-smx was no better than the placebo. | 1990 | 2193628 |
| localization of septation sites. | | 1990 | 2194250 |
| screening for neurotoxicity: complementarity of functional and morphologic techniques. | our philosophy is that screening tests should be applicable across species and emphasize complementarity to neuropathology. within this context, electrophysiological tests comparable to those in human clinical neurology are powerful screening tools. for example, while histopathologic evaluation of the cochlea for ototoxicity is difficult, evoked potential audiometry is fast and easy. in this instance, one might routinely screen for deficits in auditory function, and reserve morphologic technique ... | 1990 | 2195632 |
| [the problem of the effectiveness of condoms against hiv infection]. | in several countries the condom is recommended as the most important protection against hiv infection, although there is no proof that the condom is effective against sexually transmitted diseases (std). prior to the hiv epidemic the condom was used to prevent pregnancy and to decrease the risk of std. to prevent a deadly infection such as that with hiv, safe ways of protection are mandatory. recent studies on hiv prevention show the assumption that condoms provide reliable protection against hi ... | 1990 | 2195654 |
| [the capacity to produce cholera exotoxin in natural strains of vibrio cholerae]. | the study of 27 v. cholerae strains, isolated from cholera patients and found to be hemolytically inactive, with a view to establish their capacity for the production of cholera toxin has revealed that 4 strains (v. cholerae cholerae dacca 35, v. cholerae cholerae dacca 3, v. cholerae cholerae b1307, v. cholerae cholerae j89) produce this protein. the quantitative determination of enterotoxin has been made with the use of gm1 elisa technique. strain dacca 35 has been found to be highly toxigenic ... | 1990 | 2195813 |
| randomised double blind trial of single dose doxycycline for treating cholera in adults. | to compare the efficacy of a single dose of doxycycline (200 or 300 mg) with the standard multiple doses of tetracycline in patients with cholera. | 1990 | 2196962 |
| expression in escherichia coli of two mutated genes encoding the cholera toxin b subunit. | to allow subsequent genetically mediated fusion of foreign antigens to cholera toxin b subunit (ctb), two mutated ctb encoding genes (ctxb) were constructed and overexpressed in escherichia coli. the signal peptide coding sequence was deleted and restriction sites were created at both ends of the modified sequence. both synthesized ctbs contain additional amino acid(s) at the n terminus (one and three). they were purified as insoluble products and refolded into the natural pentameric ctb structu ... | 1990 | 2197181 |
| the essential region for assembly and particle formation in hepatitis b virus surface antigen produced in yeast cells. | the hepatitis b virus (hbv) genome carries a hbv surface antigen (hbsag) gene that can encode a polypeptide of 226 amino acids (aa). this gene can be expressed in the yeast, saccharomyces cerevisiae, and the products can be assembled into 22-nm particles indistinguishable from those recovered from a patient's serum. we constructed a set of deletion derivatives of the hbsag gene, and examined the particle-forming ability of the resulting polypeptides by expressing the gene in yeast. elimination o ... | 1990 | 2197186 |
| structural studies on the subunits of glutamate synthase from azospirillum brasilense. | the amino acid composition and the n-terminal sequences of the two dissimilar subunits of glutamate synthase from azospirillum brasilense have been determined along with the sequences of selected cnbr peptides. comparison of our data with those available for escherichia coli glutamate synthase revealed an overall good homology between the enzymes from the two sources. this is more evident for the heavy subunits where the highly conserved n-terminal sequence containing cys-1, suggests that this r ... | 1990 | 2198943 |
| isolation, characterization, and host-cell-binding properties of a cytotoxin from campylobacter jejuni. | a 68,000-molecular-weight protein was isolated by polyacrylamide gel electrophoresis from the organism-free filtrate of a fully virulent clinical strain of campylobacter jejuni. the eluted protein was heat labile, was inactivated at either ph 3.0 or 9.0, was sensitive to trypsin, and was lethal for fertile chicken eggs. it also had toxic effects on chicken embryo fibroblast, chinese hamster ovary (cho), and intestinal 407 (int407) cells. a monoclonal antibody (cetpmab4) raised to this eluted tox ... | 1990 | 2199497 |
| screening of aquatic samples for vibrio cholerae serotype o1 by a dot-blot method and a latex agglutination test. | a dot-blot, enzyme-linked immunosorbent method and a latex agglutination test were studied for their abilities to detect vibrio cholerae serotype o1 in aquatic samples by testing artificially contaminated water as well as samples from natural potential sources. water samples were preenriched with alkaline peptone and then enriched with monsur peptone water. for the dot-blot test, enriched cultures of organisms in a small portion of the monsur peptone water were transferred to a polyvinylidene di ... | 1990 | 2200338 |
| [morphological changes caused by the dermonecrosis factor of the causative agents of cholera and various other intestinal infections]. | intra- or subepithelial focal purulent inflammation with necrosis of exudating leucocytes during 1-2 days is developed in consequence of intradermal injection of the living cholera vibrios, cultured on membrane agar, or their supernatants. sometimes coagulative necrosis of cover epithelium arises without preliminary purulent inflammation stage from the very beginning. intradermal injection of living cholera vibrios leads to the development of coagulative necrotic foci in derma too. the vascular ... | 1990 | 2201411 |
| from the national institute of allergy and infectious diseases. summary of the 25th united states-japan joint conference on cholera and related diarrheal diseases. | | 1990 | 2201739 |
| development of vaccines against cholera and diarrhoea due to enterotoxigenic escherichia coli: memorandum from a who meeting. | this memorandum summarizes current knowledge on the epidemiology of cholera and diarrhoea due to enterotoxigenic escherichia coli (etec) and outlines the results of recent research to develop an effective oral vaccine against cholera. the meeting reviewed current research on the protective antigens of etec and made a number of recommendations with the aim of stimulating further efforts towards the development of vaccines against disease caused by etec. | 1990 | 2203550 |
| survival of aeromonas hydrophila and escherichia coli in aquatic environments. | survival of aeromonas hydrophila and escherichia coli in distilled water, pond water and effluents of anaerobic digesters were examined. survival capabilities of a. hydrophila in various aquatic systems were similar to e. coli except in the raw effluents of anaerobic digesters, where a. hydrophila survived significantly better than e. coli for 6 days. pathogens such as staphylococcus aureus, salmonella enteritidis and vibrio cholerae were readily killed in the raw effluents of the anaerobic dige ... | 1990 | 2203637 |
| a double-blind, controlled trial of bioflorin (streptococcus faecium sf68) in adults with acute diarrhea due to vibrio cholerae and enterotoxigenic escherichia coli. | the therapeutic efficacy of bioflorin (streptococcus faecium sf68; gipharmex, milan, italy) in acute watery diarrhea was evaluated in 183 bangladeshi adults. vibrio cholerae organisms were isolated from stool cultures in 114 patients, and enterotoxigenic escherichia coli organisms were isolated in 41. in addition to iv rehydration, patients were randomly assigned to receive either capsules of bioflorin containing 1 x 10(9) of live sf68 or capsules of placebo containing killed sf68 once every 8 h ... | 1990 | 2203662 |
| cloning and nucleotide sequence of a heat-stable enterotoxin gene from vibrio cholerae non-o1 isolated from a patient with traveler's diarrhea. | we determined the nucleotide sequence of the heat-stable enterotoxin (sta) gene of the vibrio cholerae non-o1 strain nrt36 isolated from a patient with traveler's diarrhea. the gene is chromosomally encoded and the presumed product is 78 amino acids in length, with a molecular weight of 8,814, though the genes of escherichia coli sta(s) (sth and stp) are encoded in plasmid dna and both products are 72 amino acids in length. the first 18 amino acids at the nh2 terminus were hydrophobic, suggestin ... | 1990 | 2205577 |
| vibrio cholerae hlya hemolysin is processed by proteolysis. | the leukocidal activity of the vibrio cholerae hemolysin (hlya) was utilized to detect, enrich, and clone hybridoma cells expressing neutralizing monoclonal antibody in a new survivor selection protocol. a bank of 550 hybridoma clones was obtained from a mouse immunized with hemolysin by using standard techniques. the hybridoma bank was treated with a dose of hlya hemolysin lethal to nonimmune clones. five surviving hybridoma clones (x1 through x5) which possessed anti-hlya activity were obtaine ... | 1990 | 2205579 |
| development of an in vitro model for study of non-o1 vibrio cholerae virulence using caco-2 cells. | non-o1 vibrio cholerae strains have been reported as a causative agent of diarrhea throughout the world. we recently reported that non-o1 v. cholerae strains cause diarrhea in human volunteers. in this study we evaluated the virulence of three strains of non-o1 v. cholerae in a caco-2 cell adherence assay by light and electron microscopy. a-5 is an environmental isolate which failed to colonized volunteers and did not cause diarrhea. it exhibited low numbers of organisms adherent to caco-2 cells ... | 1990 | 2205582 |
| detection of vibrio cholerae o1 in the aquatic environment by fluorescent-monoclonal antibody and culture methods. | vibrio cholerae o1 in plankton samples collected from ponds and rivers between february 1987 and january 1990 in matlab, bangladesh, was detected by the fluorescent-monoclonal antibody (fa) technique. samples were collected at sites which were monitored fortnightly (fixed sites) as well as at sites that were part of a case-control study. fa results were compared with those obtained by conventional culture methods (cm). a total of 876 samples were collected; v. cholerae o1 was detected in 563 sam ... | 1990 | 2206100 |
| 4-methylumbelliferyl-alpha-glycosides of partially o-acetylated n-acetylneuraminic acids as substrates of bacterial and viral sialidases. | 4-o-acetylated, 7-o-acetylated, and 9-o-acetylated 4-methylumbelliferyl-alpha-n-acetyl-neuraminic acids (neu4,5ac2-mu, neu5,7ac2-mu, neu5,9ac2-mu) were tested as substrates of sialidases of vibrio cholerae and of clostridium perfringens. both sialidases were unable to hydrolyse neu4,5ac2-mu. this compound at 1 mm concentration did not inhibit significantly the cleavage of neu5ac-mu, the best substrate tested. the 4-o-acetylated sialic acid glycoside is hydrolysed slowly by the sialidase from fow ... | 1990 | 2206459 |
| [experimental study on the difference of ischemic threshold between cerebral cortex and thalamus with middle latency auditory evoked potentials]. | middle latency auditory evoked potentials (ml-aeps) have not yet been established as a device of assessing neurological events because their origins have not been definitely identified. in this study, we assessed the neurological usefulness of mla-eps through experimental approach using canine models of acute ischemia localized within the cerebral cortex or thalamus. two types of localized cerebral ischemia were produced in mongrel dogs by clipping of major cerebral arteries and inducing of hypo ... | 1990 | 2206636 |
| lytic effect of vibrio cholerae elastase on gram-positive and -negative bacteria. | elastase of vibrio cholerae caused the lysis of freshly grown cells of gram-negative (pseudomonas aeruginosa, proteus vulgaris, salmonella paratyphi a and klebsiella pneumoniae) bacteria. gram-positive (staphylococcus aureus and s. epidermidis) organisms were resistant to this enzyme. heat killed and lyophilized gram-positive and -negative bacteria (except s. aureus and s. epidermidis) showed higher sensitivity to elastase. both gram-negative and -positive bacteria were lyzed maximally by elasta ... | 1990 | 2210489 |
| the effect of cefuroxime axetil on the faecal flora of healthy volunteers. | the effect on the faecal flora of cefuroxime axetil, an oral ester of cefuroxime, was examined in ten healthy volunteers following a dose of 250 mg twice a day for 41/2 days. the mean blood concentration 2 h after the ninth dose was 3.6 mg/l and the urinary excretion was approximately 40% in 6 h and 50% in 24 h. two volunteers developed mild diarrhoea and one candida vaginitis. although there was variation between volunteers in the changes found in the faecal flora, the major effects were a redu ... | 1990 | 2211457 |
| hormonal dependency of cerebral meningiomas. part 1: female sex steroid receptors and their significance as specific markers for adjuvant medical therapy. | female sex steroid receptors were examined in 50 human cerebral meningiomas. for estrogen receptors, high-affinity binding sites (dissociation constant (kd): 0.05 to 0.2 nm) were found in the cytosolic fraction with a capacity of less than 4 fmol/mg protein in 10 meningiomas using a dextran-coated charcoal (dcc) assay. in the same cytosolic fraction, the solid-phase enzyme immunoassay revealed only one cytosol with a positive colorimetric reaction equal to 5 fmol/mg protein. however, in the nucl ... | 1990 | 2213164 |
| [the composition of a population of cloned cultures of revertant vibrio cholerae l forms]. | the process of l-transformation and l-transformed state duration have been studied for their effect on variability of main characters of revertant cultures of choleric vibrions l-forms at the population level with the use of cloned cultures of the choleric vibrions. the study was conducted on two strains of the choleric vibrion of the eltor biovar in different periods of storage in the l-transformed state (1, 3, 6 months). it has been revealed that characters of the species and biovar remained s ... | 1990 | 2215283 |
| [a comparative study of the oxidation processes in the cells of typical forms and l forms of vibrio cholerae]. | a comparative study of metabolic processes in cells of typical forms and l-forms of cholera vibrios has determined a change in the intensity of oxidative processes demonstrated through a decrease in the activity of respiration enzymes (dehydrogenases and diaphorases) and through a restricted transport of electrons along the respiration chain. the facts established evidence for the transition of l-transformed cells into a physiological state with a deficit of substrates and energy as well as with ... | 1990 | 2215284 |
| penicillin binding proteins of vibrio cholerae. | eleven penicillin binding proteins (pbps) of vibrio cholerae have been identified using [125i] labelled p-hydroxybenzyl penicillin (penx). these proteins are localised in the inner membrane and have molecular weights ranging from 97,000 to 22,000. neutral hydroxylamine released the labelled penx from the pbps and pretreatment with cold benzyl penicillin inhibited labelling completely. the pbp 4 is the most sensitive target for cephaloridine and aztreonam. cephaloridine also binds to three other ... | 1990 | 2222436 |
| endogenous heparin-binding lectin activity in human placenta: purification and developmental expression. | human placental extracts contain a herapin-inhibitable lectin activity. the lectin, which closely resembles those from chicken and rat tissues, was purified by heparin-affinity chromatography. it shares many properties with the previously reported lectins, including hapten specificity, molecular weight of monomers, and immunological cross-reactivity. sections from different stages of placental development, stained by immunohistochemistry procedures using lectin-specific antibody, showed that the ... | 1990 | 2223005 |
| the involvement of the midbrain periaqueductal grey in the cardiovascular response to injury in the conscious and anaesthetized rat. | cell bodies within the periaqueductal grey (pag) of the midbrain of the propofol-anaesthetized rat were stimulated using the excitotoxin kainic acid. this resulted in a rise in arterial blood pressure and a tachycardia, together with a reduction in the sensitivity and a rightward resetting of the baroreflex. these changes mimicked the cardiovascular effect of bilateral hindlimb ischaemia in the rat. further experiments in conscious rats indicated that electrolytic lesions in the pag prevented th ... | 1990 | 2223051 |
| [cardiomyoplasty]. | the article analyses the current state of the problem of cardiomyoplasty as a new method for the treatment of patients with myocardial cardiac insufficiency. the authors show the experimental and clinical material on the possibility and efficacy of using skeletal muscles for improving myocardial contractility and raising the cardiac index. experience gained in our country (6 operations) in cardiomyoplasty in patients with severe form of ischemic heart disease is discussed. the authors used an or ... | 1990 | 2223224 |
| [cardiomyoplasty]. | the article analyses the current state of the problem of cardiomyoplasty as a new method for the treatment of patients with myocardial cardiac insufficiency. the authors show the experimental and clinical material on the possibility and efficacy of using skeletal muscles for improving myocardial contractility and raising the cardiac index. experience gained in our country (6 operations) in cardiomyoplasty in patients with severe form of ischemic heart disease is discussed. the authors used an or ... | 1990 | 2223224 |
| radioimmunotargeting of human tumour cells in immunocompetent animals. | a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ... | 1990 | 2223574 |
| radioimmunotargeting of human tumour cells in immunocompetent animals. | a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ... | 1990 | 2223574 |
| the continuous decrease in the number of sspe annual cases ten years after compulsory anti-measles immunization. | the record of sspe cases diagnosed immunochemically and serologically in our laboratory, standing for about 60% of the total new cases reported in our country, shows a significant decrease in the incidence in 1988-89 (from 5.21 new cases per year per million total population in 1987 to 1.82 cases in 1988). in 85% of the patients, sspe onset occurred at the age of 10 years or more, suggesting the possibility of a primary measles infection before anti-measles immunization became compulsory. high s ... | 1990 | 2223653 |
| the continuous decrease in the number of sspe annual cases ten years after compulsory anti-measles immunization. | the record of sspe cases diagnosed immunochemically and serologically in our laboratory, standing for about 60% of the total new cases reported in our country, shows a significant decrease in the incidence in 1988-89 (from 5.21 new cases per year per million total population in 1987 to 1.82 cases in 1988). in 85% of the patients, sspe onset occurred at the age of 10 years or more, suggesting the possibility of a primary measles infection before anti-measles immunization became compulsory. high s ... | 1990 | 2223653 |
| glucose partitioning in the pregnant ewe: effects of undernutrition and exercise. | maternal whole-body glucose entry rate and uterine and umbilical net uptakes of glucose and oxygen were measured in single-pregnant ewes which were either well-fed throughout, or fed at 0.3-0.4 predicted energy requirement for 7-21 d during late pregnancy. all ewes were studied while standing at rest and then while walking on a treadmill at 0.7 m/s on a 10 degrees slope for 60 min. underfed ewes suffered significant decreases in live weight and had lower fetal, but not placental, weights at 140- ... | 1990 | 2223746 |
| glucose partitioning in the pregnant ewe: effects of undernutrition and exercise. | maternal whole-body glucose entry rate and uterine and umbilical net uptakes of glucose and oxygen were measured in single-pregnant ewes which were either well-fed throughout, or fed at 0.3-0.4 predicted energy requirement for 7-21 d during late pregnancy. all ewes were studied while standing at rest and then while walking on a treadmill at 0.7 m/s on a 10 degrees slope for 60 min. underfed ewes suffered significant decreases in live weight and had lower fetal, but not placental, weights at 140- ... | 1990 | 2223746 |
| mechanistic studies of ionizing radiation and oxidative mutagenesis: genetic effects of a single 8-hydroxyguanine (7-hydro-8-oxoguanine) residue inserted at a unique site in a viral genome. | t4 rna ligase was used to construct a deoxypentanucleotide containing a single 8-hydroxyguanine (7-hydro-8-oxoguanine; g8-oh) residue, which is one of the putatively mutagenic dna adducts produced by oxidants and ionizing radiation. the pentamer d(gctag8-oh)p was prepared by the ligation of a chemically synthesized acceptor molecule, d(gcta), to an adducted donor, 8-hydroxy-2'-deoxyguanosine 5',3'-bisphosphate. the acceptor was efficiently converted to the reaction product (greater than 95%), an ... | 1990 | 2223758 |
| mechanistic studies of ionizing radiation and oxidative mutagenesis: genetic effects of a single 8-hydroxyguanine (7-hydro-8-oxoguanine) residue inserted at a unique site in a viral genome. | t4 rna ligase was used to construct a deoxypentanucleotide containing a single 8-hydroxyguanine (7-hydro-8-oxoguanine; g8-oh) residue, which is one of the putatively mutagenic dna adducts produced by oxidants and ionizing radiation. the pentamer d(gctag8-oh)p was prepared by the ligation of a chemically synthesized acceptor molecule, d(gcta), to an adducted donor, 8-hydroxy-2'-deoxyguanosine 5',3'-bisphosphate. the acceptor was efficiently converted to the reaction product (greater than 95%), an ... | 1990 | 2223758 |
| effect of various biophysicochemical conditions on toxigenicity of vibrio cholerae 01 during survival with a green alga, rhizoclonium fontanum, in an artificial aquatic environment. | toxigenic and nontoxigenic strains of vibrio cholerae 01 occur in the natural aquatic environment. it is not clear whether v. cholerae 01 lose toxigenicity and become nontoxigenic during survival in the aquatic environment as a result of the effect of various biophysicochemical conditions (e.g., sunlight, ph, temperature, competition with other bacteria for nutrients, etc.). five toxigenic strains were exposed to artificial aquatic environments in the presence of a filamentous green alga. rhizoc ... | 1990 | 2224645 |
| a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india. | chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ... | 1990 | 2227713 |