| biochemical characterization of four novel mutations in the thyroid hormone receptor beta gene in patients with resistance to thyroid hormone. | in resistance to thyroid hormone (rth), decreased tissue responsiveness to thyroid hormones is usually caused by mutations in the thyroid hormone receptor beta (thrb) gene. subsequently, in serum the level of thyroid stimulating hormone (tsh) is not suppressed despite increased concentrations of thyroxine (t4) and triiodothyronine (t3). in our laboratory, dna sequences of exon 7 to 10 in the thrb gene have been analysed in individuals with biochemical signs of rth. four novel point mutations wer ... | 2008 | 19378427 |
| the genome and proteome of a virulent escherichia coli o157:h7 bacteriophage closely resembling salmonella phage felix o1. | based upon whole genome and proteome analysis, escherichia coli o157:h7-specific bacteriophage (phage) wv8 belongs to the new myoviral genus, "the felix o1-like viruses" along with salmonella phage felix o1 and erwinia amylovora phage phiea21-4. the genome characteristics of phage wv8 (size 88.49 kb, mol%g+c 38.9, 138 orfs, 23 trnas) are very similar to those of phage felix o1 (86.16 kb, 39.0 mol%g+c, 131 orfs and 22 trnas) and, indeed most of the proteins have their closest homologs within feli ... | 2009 | 19379502 |
| [construction, identification of human b7.1 eukaryotic expressing vector and its expression on leukemic cells]. | the aim of study was to investigate the construction, identification and expression of human b7.1 (cd80) eukaryotic expressing vector on hl-60 cells. b7.1 gene was subcloned from the cloning vector using pcr. the pcr products and eukaryotic expressing vector (phook) both were separately digested with apai, sali and were ligated using t4 dna ligase. the ligased products were transduced into dh-5alpha. b7.1 gene containing clones was selected by digestion with apai and sali, and were further confi ... | 2009 | 19379586 |
| positron emission tomography-computed tomography in predicting locoregional invasion in esophageal squamous cell carcinoma. | in order to clarify the role of positron emission tomography-computed tomography (pet/ct) in thoracic esophageal squamous cell carcinoma we investigated its value in predicting locoregional invasion. | 2009 | 19379906 |
| pneumocephalus after resection of a lung cancer with posterior chest wall involvement. | pneumocephalus after resection of intrathoracic tumors is an extremely rare event. a delayed presentation of iatrogenic subarachnoid pleural fistula resulted in symptomatic pneumocephalus after resection of a locally recurrent t4n0 nonsmall cell lung cancer involving the chest wall, t2 to t4 rib heads, and intercostal vertebral foramina. progressive neurologic decline was noted 3 weeks after discharge. computed tomography of the head and chest confirmed the presence of an apical pleural space, t ... | 2009 | 19379924 |
| evaluation of the new tnm staging system proposed by the international association for the study of lung cancer at a single institution. | the seventh tnm classification of malignant tumours will be published in 2009. the international association for the study of lung cancer has proposed a revision of the current pathologic staging system. we illustrated the effects of this new system and pointed out potential problems using a retrospective study of surgical cases of non-small cell lung cancer at our institution. | 2009 | 19379988 |
| screening for dysfunction to promote multidisciplinary intervention by using the university of washington quality of life questionnaire. | to investigate the potential of the university of washington quality of life questionnaire (uw-qol) in routine clinics as a quick screening tool for possible dysfunction in patients after treatment of head and neck cancer. | 2009 | 19380359 |
| acceptance of major surgical procedures and quality of life among long-term survivors of advanced head and neck cancer. | to evaluate the acceptance of major surgical procedures and quality of life among long-term survivors of advanced head and neck cancer treated at a single institution. | 2009 | 19380360 |
| robot-assisted surgery for upper aerodigestive tract neoplasms. | to assess the feasibility and safety of performing robot-assisted resections of head and neck tumors, and to predict which variables lead to successful robot-assisted resection and better functional outcome. | 2009 | 19380364 |
| crystal structure of the beta beta alpha-me type ii restriction endonuclease hpy99i with target dna. | the beta beta alpha-me restriction endonuclease (rease) hpy99i recognizes the cgwcg target sequence and cleaves it with unusual stagger (five nucleotide 5'-recessed ends). here we present the crystal structure of the specific complex of the dimeric enzyme with dna. the hpy99i protomer consists of an antiparallel beta-barrel and two beta 4 alpha 2 repeats. each repeat coordinates a structural zinc ion with four cysteine thiolates in two cxxc motifs. the beta beta alpha-me region of the second bet ... | 2009 | 19380375 |
| preoperative t staging of urinary bladder cancer: does diffusion-weighted mri have supplementary value? | the objective of our study was to evaluate whether diffusion-weighted mri has supplementary value in the preoperative t staging of urinary bladder cancer. | 2009 | 19380561 |
| [gastrojejunostomy for irresectable gastric cancer with pyloric stenosis-new role of surgery in the era of s-1]. | we report a patient with an advanced gastric cancer complicated by pyloric stenosis who was effectively treated by s-1 mono-therapy after gastrojejunostomy. a 62-year-old man consulted a general practitioner for abdominal pain and anorexia. gastric roentgenography and upper gastrointestinal endoscopy showed gastric cancer(borrmann type 3) with pyloric stenosis. he was referred to our department. he underwent laparotomy, which revealed a t4 tumor invading the pancreas head, but neither liver nor ... | 2009 | 19381039 |
| treatment and outcome of advanced external auditory canal and middle ear squamous cell carcinoma. | this is a retrospective study to evaluate the outcomes and complications of combined treatment, surgery with or without adjunctive intraoperative radiotherapy, of locally advanced temporal bone squamous cell carcinoma. a series of 17 patients with locally advanced squamous cell carcinoma of the temporal bone were treated between september 2002 and february 2007. eleven patients had primary tumors, and 6 patients had recurrences. according to the university of pittsburgh staging system, 5 patient ... | 2009 | 19381105 |
| effects of thyroid status on arginine vasotocin receptor vt2r expression and adrenal function in osmotically stimulated domestic fowl. | the role of thyroid hormones in the regulation of adrenal function during stress has been documented in mammals, but only limited reports are available in avian species. the present study was undertaken to analyze the effect of hyper- or hypothyroidism on the adrenal activity under control (hydrated) and osmotically stressed (water deprived, wd) conditions, with special emphasis on the expression of arginine vasotocin receptor vt2 (vt2r) in pituitary corticotrophs. chickens were made hyper- or h ... | 2009 | 19381646 |
| the zn center of the anaerobic ribonucleotide reductase from e. coli. | strict and facultative anaerobes depend on a class iii ribonucleotide reductase for their growth. these enzymes are the sole cellular catalysts for de novo biosynthesis of the deoxyribonucleotides needed for dna chain elongation and repair. in its active form, the class iii ribonucleotide reductase from escherichia coli contains a free radical located on the g681 residue which is essential for the activation of the ribonucleotide substrate toward its reduction. the 3d structure of the homologous ... | 2009 | 19381696 |
| methods to study transcription-coupled repair in chromatin. | transcription-coupled repair (tcr) is a sub-pathway of nucleotide excision repair that allows for the enhanced repair of the transcribed strand of active genes. a classical method to study dna repair in vivo consists in the molecular analysis of uv-induced dna damages at specific loci. cells are irradiated with a defined dose of uv light leading to the formation of dna lesions and incubated in the dark to allow repair. about 90% of the photoproducts consist of cyclobutane pyrimidine dimers, whic ... | 2009 | 19381941 |
| does the circadian rhythm of melatonin affect ischemia-reperfusion injury after coronary artery bypass grafting? | melatonin is a potent scavenger of free radicals and an antioxidant. we studied the relationship between the protective effect of melatonin against ischemia-reperfusion injury (iri) during cardiopulmonary bypass, the plasma level of melatonin, and the time of surgery. | 2009 | 19383595 |
| evaluation at atomic resolution of the role of strain in destabilizing the temperature-sensitive t4 lysozyme mutant arg 96 --> his. | mutant r96h is a classic temperature-sensitive mutant of bacteriophage t4 lysozyme. it was in fact the first variant of the protein to be characterized structurally. subsequently, it has been studied extensively by a variety of experimental and computational techniques, but the reasons for the loss of stability of the mutant protein remain controversial. in the crystallographic refinement of the mutant structure at 1.9 a resolution one of the bond angles at the site of substitution appeared to b ... | 2009 | 19384984 |
| contributions of all 20 amino acids at site 96 to the stability and structure of t4 lysozyme. | to try to resolve the loss of stability in the temperature-sensitive mutant of t4 lysozyme, arg 96 --> his, all of the remaining 18 naturally occurring amino acids were substituted at site 96. also, in response to suggestions that the charged residues lys85 and asp89, which are 5-8 a away, may have important effects, each of these amino acids was replaced with alanine. crystal structures were determined for many of the variants. with the exception of the tryptophan and valine mutants r96w and r9 ... | 2009 | 19384988 |
| structural origin of weakly ordered nitroxide motion in spin-labeled proteins. | a disulfide-linked nitroxide side chain (r1) used in site-directed spin labeling of proteins often exhibits an epr spectrum characteristic of a weakly ordered z-axis anisotropic motion at topographically diverse surface sites, including those on helices, loops and edge strands of beta-sheets. to elucidate the origin of this motion, the first crystal structures of r1 that display simple z-axis anisotropic motion at solvent-exposed helical sites (131 and 151) and a loop site (82) in t4 lysozyme ha ... | 2009 | 19384990 |
| validation of spinal motion with the spine reposition sense device. | a sagittal plane spine reposition sense device (srsd) has been developed. two questions were addressed with this study concerning the new srsd: 1) whether spine movement was occurring with the methodology, and 2) where movement was taking place. | 2009 | 19386126 |
| treatment of nasopharyngeal carcinoma using intensity-modulated radiotherapy-the national cancer centre singapore experience. | the aim of this study was to determine the efficacy and acute toxicity of our early experience with treating nasopharyngeal carcinoma (npc) patients with intensity-modulated radiotherapy (imrt). | 2009 | 19386431 |
| a prognostic index for predicting lymph node metastasis in minor salivary gland cancer. | large studies examining the clinical and pathological factors associated with nodal metastasis in minor salivary gland cancer are lacking in the literature. | 2010 | 19386433 |
| carbohydrate analysis of acanthamoeba castellanii. | we analyzed biochemically acanthamoeba castellanii trophozoites, intact cysts and cyst walls belonging to the t4 genotype using gas chromatography combined with mass spectrometry. cyst walls were prepared by removing intracellular material from cysts by pre-treating them with sodium dodecyl sulphate (sds) containing dithiothreitol, and then subjecting these to a series of sequential enzymatic digestions using amyloglucosidase, papain, dnase, rnase and proteinase k. the resulting "cyst wall" mate ... | 2009 | 19389397 |
| caspase-cleaved tau expression induces mitochondrial dysfunction in immortalized cortical neurons: implications for the pathogenesis of alzheimer disease. | in alzheimer disease (ad) mitochondrial abnormalities occur early in the pathogenic process and likely play a significant role in disease progression. tau is a microtubule-associated protein that is abnormally processed in ad, and a connection between tau pathology and mitochondrial impairment has been proposed. however, few studies have examined the relationship between pathological forms of tau and mitochondrial dysfunction. we recently demonstrated that inducible expression of tau truncated a ... | 2009 | 19389700 |
| phenotypic and functional characterization of human mammary stem/progenitor cells in long term culture. | cancer stem cells exhibit close resemblance to normal stem cells in phenotype as well as function. hence, studying normal stem cell behavior is important in understanding cancer pathogenesis. it has recently been shown that human breast stem cells can be enriched in suspension cultures as mammospheres. however, little is known about the behavior of these cells in long-term cultures. since extensive self-renewal potential is the hallmark of stem cells, we undertook a detailed phenotypic and funct ... | 2009 | 19390630 |
| compensatory sweating after thoracoscopic sympathectomy: characteristics, prevalence and influence on patient satisfaction. | this prospective study aimed at investigating predictive factors for compensatory sweating after thoracoscopic sympathectomy. | 2009 | 19390718 |
| analysis of mel-18 expression in prostate cancer tissues and correlation with clinicopathologic features. | mel-18 is a member of the polycomb group (pcg) of proteins, which are chromatin regulatory factors that play an important role in development and oncogenesis. this study was designed to investigate the clinical and prognostic significance of mel-18 in the patients with prostate cancer. | 2011 | 19395284 |
| selenium and thyroid hormone axis in critical ill states: an overview of conflicting view points. | in critical ill states the plasma selenium levels are low and inversely correlated with the severity and outcome of the disease. the plasma selenium levels indicate the amount of circulating selenoproteins and selenoenzymes. these are important for the maintenance of the redox system, modulating the immune system and also for thyroid hormone metabolism. not only all three deiodinases (d1-3) are selenoenzymes, but within the thyroid gland there are several other selenoenzymes, which are important ... | 2009 | 19398053 |
| surgical management of superficial siderosis. | superficial siderosis is a rare condition resulting from the presence of chronic bleeding into the subarachnoid space usually causing gait instability and deafness. the surgical management of superficial siderosis depends on localizing the source of hemorrhage. | 2009 | 19398385 |
| pleural malignant mesothelioma causing cord infiltration through the nerve root. case report. | a 61-year-old man presented with a rare pleural malignant mesothelioma of the spine manifesting as progressive weakness of the bilateral lower extremities, numbness in the body and both legs, and dysfunction of the bladder and bowel. he had previous occupational exposure to asbestos while working at a car repair shop and had undergone right panpleuropneumonectomy under a diagnosis of sarcomatous type mesothelioma in the right pleural space. magnetic resonance imaging of the spine with gadolinium ... | 2009 | 19398862 |
| quantitative thyroid scintigraphy in greyhounds suspected of primary hypothyroidism. | the existence of hypothyroidism in greyhounds remains controversial and its investigation is complicated by the low circulating thyroid hormone concentrations typically found in healthy dogs of this breed. quantitative measurement of thyroidal technetium-99m pertechnetate ((99m)tco4-) uptake is known to be useful in assessing thyroid function in other breeds. the aim of this study was to evaluate thyroid scintigraphy as a method of assessing thyroid function in greyhounds suspected of primary hy ... | 2009 | 19400473 |
| the prevalence of thyroid dysfunction in an iodine-sufficient area in iran. | the prevalence of hypothyroidism in iran is unknown. the aims of the present study were to estimate the prevalence of overt and subclinical hypothyroidism among the adult population of isfahan, a large metropolitan city in iran, 15 years after universal salt iodization. | 2009 | 19400604 |
| comparison of the effect of propofol and n-acetyl cysteine in preventing ischaemia-reperfusion injury. | the aim of this study was to compare the effects of propofol and n-acetyl cysteine (nac) on tourniquet-induced ischaemia-reperfusion injury by determining malonyldialdehyde, ischaemia-modified albumin, lactate, blood gas and haemodynamic levels in arthroscopic knee surgery. | 2009 | 19401659 |
| effects of selenomethionine supplementation on selenium status and thyroid hormone concentrations in healthy adults. | selenium, a potential cancer prevention agent currently being tested against prostate cancer in the selenium and vitamin e cancer prevention trial (select), plays an integral role in thyroid metabolism. the effects of long-term selenium supplementation on thyroid hormone concentrations are unknown. | 2009 | 19403637 |
| resistance of acanthamoeba cysts to disinfection in multiple contact lens solutions. | acanthamoebae are free-living amoebae found in the environment, including soil, freshwater, brackish water, seawater, hot tubs, and jacuzzis. acanthamoeba species can cause keratitis, a painful vision-threatening infection of the cornea, and fatal granulomatous encephalitis in humans. more than 20 species of acanthamoeba belonging to morphological groups i, ii, and iii distributed in 15 genotypes have been described. among these, acanthamoeba castellanii, a. polyphaga, and a. hatchetti are frequ ... | 2009 | 19403771 |
| acute chest pain in a top soccer player due to thoracic disc herniation. | case report. | 2009 | 19404165 |
| management of squamous cell carcinoma of the base of tongue with chemoradiation and brachytherapy. | treatment of base of tongue (bot) squamous cell carcinoma (scc) has traditionally been associated with poor prognosis and significant morbidity. we report a program consisting of concurrent chemoradiation followed by brachytherapy for these patients. | 2009 | 19405086 |
| [results of alginate and hypertonic solution in wound healing of head and neck cancers]. | in head and neck cancers, complications such as pharyngostoma, orostoma, or fistula can present following surgery or surgery after irradiation. | 2008 | 19408512 |
| epidemiology and clinical characteristics of thyroid dysfunction in children and adolescents with type 1 diabetes. | the aim of the study was to evaluate the natural course and potential risk factors of autoimmune thyroiditis (ait) and thyroid dysfunction, and their influences on growth and glycemic control in children and adolescents with type 1 diabetes mellitus (t1d). the study comprised 148 subjects (age range 1-21 years; males 51%) with t1d. during the interval of 12 years serum levels of thyroid peroxidase (anti-tpo) and thyroglobulin (anti-tg) autoantibodies, thyroid-stimulating hormone (tsh) and tyroks ... | 2009 | 19408637 |
| different behaviors in vivo of mutations in the beta hairpin loop of the dna polymerases of the closely related phages t4 and rb69. | the t4 and rb69 dna replicative polymerases are members of the b family and are highly similar. both replicate dna with high fidelity and employ the same mechanism that allows efficient switching of the primer terminus between the polymerase and exonuclease sites. both polymerases have a beta hairpin loop (hereafter called the beta loop) in their exonuclease domains that plays an important role in active-site switching. the beta loop is involved in strand separation and is needed to stabilize pa ... | 2009 | 19409904 |
| thyroid autoimmunity and thyroid hormone reference intervals in apparently healthy chinese adults. | thyroid disorders are common, often go unrecognized, and when left untreated, can have serious public health implications. dietary and ethnic differences are known to influence thyroid function. however, comparatively few studies have examined the prevalence of autoimmune thyroid disease (aitd) and thyroid function test (tft) reference intervals in non-western populations. | 2009 | 19410568 |
| reconstitution of uracil dna glycosylase-initiated base excision repair in herpes simplex virus-1. | herpes simplex virus-1 is a large double-stranded dna virus that is self-sufficient in a number of genome transactions. hence, the virus encodes its own dna replication apparatus and is capable of mediating recombination reactions. we recently reported that the catalytic subunit of the hsv-1 dna polymerase (ul30) exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities that are integral to base excision repair. base excision repair is required to maintain genome stability as ... | 2009 | 19411250 |
| ghrelin response to oral glucose load in hyperthyroidism, before and after treatment with antithyroid drugs. | hyperthyroidism is characterized by hyperphagia and increased basal metabolic rate. ghrelin peptide is implicated in food intake through activation of the orexigenic neuropeptide y/agouti related protein in the arcuate nucleus of hypothalamus. also different studies suggested that ghrelin might play a role in states of energy insufficiency, controlling body weight. we therefore evaluate ghrelin levels in severe hyperthyroidism before and after medical treatment when euthyroidism was achieved, in ... | 2009 | 19411802 |
| radiometabolic treatment of hyperthyroidism with a calculated dose of 131-iodine: results of one-year follow-up. | oral administration of radioactive iodine (131i) is a well-known and effective procedure for the treatment of hyperthyroidism. however, the optimal dose is still a matter of debate, as is the frequency of recurrence and hypothyroidism. the aim of our study was to evaluate the 1-yr outcome of a calculated dose of 131i activity in the treatment of hyperthyroidism, following the guidelines published jointly by the italian society of endocrinology and the italian society of nuclear medicine.we studi ... | 2009 | 19411811 |
| the effect of lead intoxication on endocrine functions. | studies on the effects of lead on the endocrine system are mainly based on occupationally lead-exposed workers and experimental animal models. although evidence is conflicting, it has been reported that accumulation of lead affects the majority of the endocrine glands. in particular, it appears to have an effect on the hypothalamic-pituitary axis causing blunted tsh, gh, and fsh/lh responses to trh, ghrh, and gnrh stimulation, respectively. suppressed gh release has been reported, probably cause ... | 2009 | 19411819 |
| functional and topographic segregation of glomeruli revealed by local staining of antennal sensory neurons in the honeybee apis mellifera. | in the primary olfactory center of animals, glomeruli are the relay stations where sensory neurons expressing cognate odorant receptors converge onto interneurons. in cockroaches, moths, and honeybees, sensory afferents from sensilla on the anterodorsal surface and the posteroventral surface of the flagellum form two nerves of almost equal thicknesses. in this study, double labeling of the two nerves, or proximal/distal regions of the nerves, with fluorescent dyes was used to investigate topogra ... | 2009 | 19412930 |
| the surgical dilemma of 'functional inoperability' in oral and oropharyngeal cancer: current consensus on operability with regard to functional results. | if surgical resection of a tumour results in an unacceptable loss of function, this is defined as 'functional inoperability'. the current survey aims to define the borders of functional inoperability in oral and oropharyngeal carcinoma and evaluate its current use by obtaining opinions from the experts in the field. | 2009 | 19413612 |
| synthesis of atp derivatives of compounds of the mevalonate pathway (isopentenyl di- and triphosphate; geranyl di- and triphosphate, farnesyl di- and triphosphate, and dimethylallyl diphosphate) catalyzed by t4 rna ligase, t4 dna ligase and other ligases potential relationship with the effect of bisphosphonates on osteoclasts. | compounds of the mevalonate pathway containing a terminal di- or triphosphate (mev-pp or mev-ppp) were tested as substrates of several enzyme ligases (t4 rna ligase, t4 dna ligase, firefly luciferase and other ligases) for the synthesis of atp derivatives of the mev-pppa or mev-ppppa type. t4 rna ligase, in the presence of atp and the substrates: geranyl, farnesyl or isopentenyl triphosphates, and geranyl, farnesyl, dimethylallyl or isopentenyl diphosphates, all at 0.3 mm concentration, catalyze ... | 2009 | 19414000 |
| structural consequences of a 3'-->3' dna interstrand cross-link by a trinuclear platinum complex: unique formation of two such cross-links in a 10-mer duplex. | combined multidimensional nuclear magnetic resonance spectroscopy and electrospray mass spectrometry was used to analyze the platinated dna adduct of the phase ii anticancer drug [[trans-ptcl(nh(3))(2)](2)-mu-[trans-pt(nh(3))(2)(nh(2)(ch(2))(6)nh(2))(2)]](no(3))(4) (bbr3464) with [5'-d(acg*tatacg*t)-3'](2). two 1,2-interstrand cross-links were formed by concomitant binding of two trinuclear moieties to the oligonucleotide. the four dna-bound platinum atoms coordinated in the major groove at n7 p ... | 2009 | 19415349 |
| a novel variant in serpina7 gene in a family with thyroxine-binding globulin deficiency. | thyroxine-binding globulin (tbg) carries approximately 75% of serum t4 and t3. this protein is encoded by serpina7 gene, formerly known as tbg gene, localized on x-chromosome (xq22.2). a deficiency in tbg is suspected when abnormally low serum total t4 and t3 are encountered in clinically euthyroid subjects in the presence of normal serum tsh. this condition has been associated with different serpina7 gene mutations resulting in amino acid substitutions or truncations in the mature protein. here ... | 2009 | 19415532 |
| 23rd anniversary of percutaneous laser disc decompression (pldd). | in mid-february 1986, peter ascher and daniel choy performed the first percutaneous laser disc decompression (pldd) procedure at the neurosurgical department, university of graz, graz, austria. it was planned to deliver 1000 j of energy with an nd:yag laser to a herniated l4-l5 disc causing sciatica. at 600 j the procedure was terminated because the pain was gone. | 2009 | 19416003 |
| relationship of hormonal resuscitation therapy and central venous pressure on increasing organs for transplant. | hormonal resuscitation therapy (hrt) has been shown to increase the number of organs available for transplant. likewise, optimal fluid balance, as measured by central venous pressure (cvp), impacts the function of donor lungs. the purpose of this study is to examine the interplay of these two variables in donor management and the impact they have on organs transplanted, with particular emphasis on hearts and lungs. | 2009 | 19416777 |
| antitumor activity of endogenous mflt4 displayed on a t4 phage nanoparticle surface. | flt4 plays a key role in promoting tumor metastasis by stimulating solid tumor lymphangiogenesis. in this study, mouse flt4 (mflt4) was displayed on t4 phage in order to explore the feasibility of breaking immune tolerance to "self-antigens" and to evaluate the phage's antitumor activity. | 2009 | 19417736 |
| [how to prescribe thyroid hormones]. | thyroid substitution is generally considered easy as well by general practitioners as by specialists, considering that a single hormone levothyroxin is recommended and that laboratory tests are readily available for measurement of free t4 and tsh. however cross sectional studies have shown that about 45% of patients are over-treated and under-treated. this paper summarizes the critical information useful to facilitate a better management of hypothyroid patients by promoting long lasting euthyroi ... | 2009 | 19418976 |
| relationship between free t4 levels and postnatal steroid therapy in preterm infants. | transient hypothyroxinemia is the most common thyroid dysfunction in preterm infants. hypothalamic-pituitary-thyroid immaturity and non-thyroidal illness contribute to its etiology. the aim of the present study was therefore to determine the relationship between thyroid hormone status and early postnatal steroid therapy in preterm infants. | 2009 | 19419509 |
| juvenile onset systemic lupus erythematosus thyroid dysfunction: a subgroup with mild disease? | the aim of this study was to evaluate the frequency of thyroid dysfunction and thyroid antibodies in patients with juvenile onset systemic lupus erythematosus (josle) and its association with clinical and immunological features. seventy-seven patients with josle, 64 females, median age 19 years, were consecutively enrolled from march to december 2007. clinical data related to thyroid dysfunction and lupus were obtained by chart review and patient interview. serum levels of tsh, free t4, anti-thy ... | 2009 | 19419839 |
| reproductive and developmental toxicity of a pentabrominated diphenyl ether mixture, de-71, to ranch mink (mustela vison) and hazard assessment for wild mink in the great lakes region. | polybrominated diphenyl ethers (pbdes) are widespread persistent and bioaccumulative environmental contaminants. recent scientific attention has focused on the developmental toxicity of pbde commercial mixtures following perinatal exposure of rodents; however, these studies do not necessarily predict toxicity to highly exposed top predators, such as mink (mustela vison). here we assessed the effects of environmentally relevant doses (0, 0.1, 0.5, and 2.5 ppm [wt/wt] in feed) of a technical penta ... | 2009 | 19420013 |
| vaccinia virus g8r protein: a structural ortholog of proliferating cell nuclear antigen (pcna). | eukaryotic dna replication involves the synthesis of both a dna leading and lagging strand, the latter requiring several additional proteins including flap endonuclease (fen-1) and proliferating cell nuclear antigen (pcna) in order to remove rna primers used in the synthesis of okazaki fragments. poxviruses are complex viruses (dsdna genomes) that infect eukaryotes, but surprisingly little is known about the process of dna replication. given our previous results that the vaccinia virus (vacv) g5 ... | 2009 | 19421403 |
| reconstruction of extensive posterior mid-thoracic soft-tissue defects after spinal surgery on irradiated skin. | a 56-year-old patient presented with neurological disorders resulting from an extrinsic medullary compression extending from t3 to t5 associated with a t4 corporeal invasion by a high-grade non-hodgkin's malignant lymphoma. treatment consisted in a laminectomy without spinal stabilization followed immediately by chemotherapy (vcad) and locoregional radiotherapy (20 cycles of 50 gy between t3 and t7 followed by nine sequences of 18 gy). the evolution revealed unfavourable with reappearance of a c ... | 2009 | 19423420 |
| pattern of use of adjuvant chemotherapy for stage ii colon cancer: a single-institution experience. | purpose: american society of clinical oncology (asco) guidelines define high-risk prognostic features (hrfs) in stage ii colon cancer and recommend limiting adjuvant chemotherapy to patients with hrfs. we evaluated the extent to which hrfs influenced decisions on adjuvant chemotherapy before and after publication of the guidelines. patients and methods: we reviewed data from 100 consecutive patients with stage ii colon cancer resected between january 2000 and june 2007. practice pattern in the p ... | 2009 | 19423502 |
| expression analysis of vegf-a and vegf-b: relationship with clinicopathological parameters in bladder cancer. | the present investigation was conducted first to determine whether correlation exists between vegf-a and -b mrna levels and clinicopathological parameters and to assess their prognostic value in bladder cancer, then to clarify the expression level and biological significance of vegf-a isoforms. total rna was isolated from 37 specimens of bladder cancer. northern blot analysis revealed that vegf-b mrna was not expressed either in normal urothelium or in bladder cancer and detected three vegf-a tr ... | 2009 | 19424629 |
| a population-based study of patterns of care for muscle-invasive bladder cancer in sweden. | to analyse the management of muscle-invasive bladder cancer in a population-based national register, and specifically to investigate the role of curative therapy (i.e. cystectomy or radiotherapy) in relation to patient, tumour and hospital characteristics. | 2009 | 19424934 |
| heterogeneity of systemic inflammatory responses to periodontal therapy. | we investigated the effect of comprehensive periodontal therapy on the levels of multiple systemic inflammatory biomarkers. | 2009 | 19426174 |
| the effect of pneumoperitoneum in the steep trendelenburg position on cerebral oxygenation. | davinci robot-assisted laparoscopic radical prostatectomy (ralp) requires pneumoperitoneum in the steep trendelenburg position, which results in increased intracranial pressure and cerebral blood flow. the aim of this study was to evaluate the effect of pneumoperitoneum in a 30 degrees trendelenburg position on cerebral oxygenation using regional cerebral oxygen saturation (rso2). | 2009 | 19426238 |
| evidence for transcriptase quantum processing implies entanglement and decoherence of superposition proton states. | evidence requiring transcriptase quantum processing is identified and elementary quantum methods are used to qualitatively describe origins and consequences of time-dependent coherent proton states populating informational dna base pair sites in t4 phage, designated by g-c-->g'-c', g-c-->*g-*c and at-->*a-*t. coherent states at these 'point' dna lesions are introduced as consequences of hydrogen bond arrangement, keto-amino-->enol-imine, where product protons are shared between two sets of indis ... | 2009 | 19427355 |
| external beam radiotherapy plus 24-hour continuous infusion of gemcitabine in unresectable pancreatic carcinoma: long-term results of a phase ii study. | to evaluate the efficacy of gemcitabine-based chemoradiation (ct-rt) in treating patients (pts) affected by locally advanced pancreatic cancers (lapc). | 2010 | 19427747 |
| immunoprotective analysis of vhhp2, a vibrio harveyi vaccine candidate. | vhhp2 is an outer membrane protein identified in a pathogenic vibrio harveyi strain, t4, isolated from diseased fish. when used as a subunit vaccine, purified recombinant vhhp2 affords high level of protection upon japanese flounder against v. harveyi challenge. vaccination with vhhp2 induced the expression of a number of immune-related genes, especially those encoding immunoglobulin m (igm) and major histocompatibility complex (mhc) iialpha. a vhhp2 surface display system, in the form of the fi ... | 2009 | 19428886 |
| hsp90b1, a thyroid hormone-responsive heat shock protein gene involved in photoperiodic signaling. | in order to further advance the understanding of genes involved in avian photoperiodic signaling, a chicken hypothalamic cdna microarray was made to identify changes in gene expression in the whole hypothalamus of juvenile male domestic chickens after 4 days' photostimulation. the most robust change was a depression in heat shock protein 90b1 (hsp90b1) expression. this observation was confirmed using quantitative pcr, and it was subsequently demonstrated that the depression in hsp90b1 expression ... | 2009 | 19429192 |
| spinal anesthesia with isobaric tetracaine in patients with previous lumbar spinal surgery. | previous lumbar spinal surgery (plss) is not currently considered as a contraindication for regional anesthesia. however, there are still problems that make spinal anesthesia more difficult with a possibility of worsening the patient's back pain. spinal anesthesia using combined spinal-epidural anesthesia (csea) in elderly patients with or without plss was investigated and the anesthetic characteristics, success rates, and possible complications were evaluated. | 2009 | 19430559 |
| kinetics of the t4 gene 32 protein-single-stranded nucleic acid interaction. | | 1980 | 19431394 |
| fluctuations in the velocity of individual dna molecules during agarose gel electrophoresis. | the velocity of the center of mass of individual t4 dna molecules during agarose gel electrophoresis, computed from digitized video-microscopic images, fluctuated between 0 and 4.5 mum/s after a field e = 5 v/cm was applied; the amplitude of the velocity peaks was twice the averaged steady-state velocity. the velocity fluctuations correlated with changes in molecular configuration. the mean velocity (10 molecules) showed a sharp rise in less than 0.2 s, followed by a shallow minimum and a broad ... | 1992 | 19431863 |
| structural properties and antibacterial effects of hydrophobic and oleophobic sol-gel coatings for cotton fabrics. | in a continuation of previous studies, the wetting properties of the hydrophobic diureapropyltriethoxysilane [bis(aminopropyl)-terminated polydimethylsiloxane (1000)] (pdmsu) sol-gel hybrid, which forms washing-resistant water-repellent finishes on cotton fabrics, were further investigated. the addition of 1h,1h,2h,2h-perfluorooctyltriethoxysilane (pfotes) to pdmsu resulted in a highly apolar low-energy surface on aluminum with gammastotal equal to 14.5 mj/m2 and a detlagiwi value of -82 mj/m2. ... | 2009 | 19432495 |
| involvement of pgc-1, nrf-1, and nrf-2 in metabolic response by rat liver to hormonal and environmental signals. | we studied liver oxidative capacity and o2 consumption in hypothyroid rats treated for 10 days with t4, or t3, or treated for 10 days with t3 and exposed to cold for the last 2 days. the metabolic response of homogenates and mitochondria indicated that all treatments increased the synthesis of respiratory chain components, whereas only the cold-induced mitochondrial proliferation. determination of mrna and protein expression of transcription factor activators, such as nrf-1 and nrf-2, and coacti ... | 2009 | 19433258 |
| thyroid hormone: a "prime suspect" in human immunodeficiency virus (hiv/aids) patients? | acquired immunodeficiency syndrome (aids) is the final and most serious stage of the disease caused by human immunodeficiency virus. the immune system is the target of aids. we investigate presently any possible involvement of thyroid hormone, the deficiency of which gives rise to oedema and susceptibility to nonspecific infections; with a view to finding the primary factor seeding the disease. it has been reported that circumcision reduced the incidence of hiv/aids infection. beyond circumcisio ... | 2008 | 19434216 |
| adjustment of n stages of gastric cancer by the ratio between the metastatic and examined lymph nodes. | the present study attempted to determine whether n stage could be adjusted for the differences in survival rates based on the ratio between the examined and metastatic lymph nodes (ln ratio). | 2009 | 19434459 |
| [expression of galectin 3 and sambucus nigra agglutinin and their clinicopathological significance in benign and malignant lesions of stomach]. | to study the expressive levels of galectin-3(gal-3) and sambucus nigra agglutinin(sna) and their clinicopathological significance in the benign and malignant lesions of stomach. | 2009 | 19434543 |
| photo-assisted gene delivery using light-responsive catanionic vesicles. | photoresponsive catanionic vesicles have been developed as a novel gene delivery vector combining enhanced cellular uptake with phototriggered release of vesicle payload following entry into cells. vesicles with diameters ranging from 50 to 200 nm [measured using cryo-transmission electron microscopy (tem) and light-scattering techniques] form spontaneously, following mixing of positively charged azobenzene-containing surfactant and negatively charged alkyl surfactant species. fluorescent probe ... | 2009 | 19435291 |
| overt and subclinical thyroid dysfunction among indian pregnant women and its effect on maternal and fetal outcome. | thyroid disorders are among the common endocrine problems in pregnant women. it is now well established that not only overt, but subclinical thyroid dysfunction also has adverse effects on maternal and fetal outcome. there are few data from india about the prevalence of thyroid dysfunction in pregnancy. with this background, this study aims to find prevalence of thyroid dysfunction in pregnancy and its impact on obstetrical outcome in indian population. | 2010 | 19437026 |
| evaluation of new thiadiazoles and benzothiazoles as potential radioprotectors: free radical scavenging activity in vitro and theoretical studies (qsar, dft). | thiol and aminothiol compounds are among the most efficient chemical radioprotectors. to increase their efficiency, we synthesized two new classes of thiol and aminothiol compounds derived from benzothiazole (t1, t2, am1, am2) and thiadiazole (t3, t4, am3) structures. we examined them for their ability to scavenge free radicals (dpph*, abts(*+), *oh). thiol derivatives with a thiadiazole structure are the most active compounds scavenging dpph* and abts(*+) free radicals, with an ic(50) of 0.053+ ... | 2009 | 19439222 |
| enantioselective determination of thyroxine enantiomers by ligand-exchange ce with uv absorbance and icp-ms detection. | a simple ce method has been developed for the separation and determination of thyroxine (t4) enantiomers in pharmaceutical formulations. the method was based on ligand-exchange mechanism using a cu(ii)/l-proline complex as chiral selector. the effects of different parameters affecting separation such as chiral selector concentration, organic additive, buffer ph and temperature were investigated. a baseline separation of the two enantiomers was obtained at a cu(ii)/l-proline ratio of 1:8 in a bor ... | 2009 | 19441034 |
| cyp1a2*1c, cyp2e1*5b, and gstm1 polymorphisms are predictors of risk and poor outcome in head and neck squamous cell carcinoma patients. | head and neck squamous cell carcinoma (hnscc) is associated with environmental factors, especially tobacco and alcohol consumption. most of the carcinogens present in tobacco smoke are converted into dna-reactive metabolites by cytochrome p450 (cyps) enzymes and detoxification of these substances is performed by glutathione s-transferases (gsts). it has been suggested that genetic alterations, such as polymorphisms, play an important role in tumorigenesis and hnscc progression. the aim of this s ... | 2009 | 19442564 |
| clinical and pathological response to primary chemotherapy in patients with locally advanced breast cancer grouped according to hormonal receptors, her2 status, grading and ki-67 proliferation index. | biological markers that reliably predict clinical and pathological response to primary systemic therapy may have considerable clinical potential; this study evaluated response compared to expression of er, pgr and her2, grading and ki-67 proliferation index before and after neoadjuvant chemotherapy in patients with locally advanced breast cancer (labc). | 2009 | 19443376 |
| structural basis for the nuclease activity of a bacteriophage large terminase. | the dna-packaging motor in tailed bacteriophages requires nuclease activity to ensure that the genome is packaged correctly. this nuclease activity is tightly regulated as the enzyme is inactive for the duration of dna translocation. here, we report the x-ray structure of the large terminase nuclease domain from bacteriophage spp1. similarity with the rnase h family endonucleases allowed interactions with the dna to be predicted. a structure-based alignment with the distantly related t4 gp17 ter ... | 2009 | 19444313 |
| the effect of treatment on survival in patients with advanced laryngeal carcinoma. | over the last 2 decades, survival from laryngeal cancer has decreased. we sought to identify factors associated with decreased survival in laryngeal cancer. | 2009 | 19444887 |
| [ct evaluation of locoregional spread of carcinoma of the gallbladder]. | this study aims to assess the diagnostic capability of ct to preoperatively detect evidence of locoregional spread of gallbladder cancer. | 2009 | 19446691 |
| preparation of long sticky ends for universal ligation-independent cloning: sequential t4 dna polymerase treatments. | ligation-independent cloning (lic) is a useful method for efficient directional cloning of a pcr product. lic requires a specially designed vector containing a long stretch of sequence that is missing any one of the four nucleotides. when the linearized vector is treated with t4 dna polymerase, in the presence of the absent base, long single-stranded overhangs are generated that are suitable for cloning. in this study, long and efficient sticky ends for lic were produced by sequential t4 dna pol ... | 2009 | 19447347 |
| accuracy of sentinel node biopsy in breast cancer patients with a high prevalence of axillary metastases. | sentinel lymph node biopsy (slnb) has become a standard diagnostic tool for axillary staging of breast cancer and has many advantages in comparison with axillary dissection. the goal of our study was to determine accuracy and predictive value of slnb for assessing the axillary lymph nodes involvement in breast cancer. | 2009 | 19447739 |
| thyroid function in the critically ill newborn and child. | here we review typical thyroid function alterations observed in the critically ill pediatric patient. | 2009 | 19448543 |
| turner's syndrome and subclinical autoimmune thyroid disease: a two-year follow-up study. | although autoimmune thyroid disease (aitd) is frequent in turner's syndrome (ts), followup studies are scant, and there are none regarding subclinical thyroiditis. we investigated thyroid function and morphology in 17 patients with ts (mean age 14.6 years) with transient and asymptomatic variations of tsh and/or thyroid hormones. our 2-year follow-up included measurements of tsh, free t4, t3 and tpo and tg antibodies, ultrasound (us) (first and last evaluations) and scintigraphy (first evaluatio ... | 2009 | 19449668 |
| treatment results and late complications of 556 patients with locally advanced nasopharyngeal carcinoma treated with radiotherapy alone. | the aim of this study was to investigate the outcome in 556 patients with locally advanced nasopharyngeal carcinomas treated by radiation therapy alone. we observed 556 patients with stage t3-4 and n0-3 carcinoma who were treated by conventional radiotherapy alone between january and december 1999. the total dose delivered to the nasopharynx was 66-80 gy over 6.5-8 weeks and to the neck lymph nodes 60-70 gy over 6-7 weeks. the 5-year actuarial overall survival rate (os) reached 66.41%. the os wa ... | 2009 | 19451314 |
| effect of treadmill training on autonomic dysreflexia in spinal cord--injured rats. | weight-supported treadmill training is an emerging rehabilitation method used to improve locomotor ability in patients with spinal cord injury (sci). however, little research has been undertaken to test the effect of such training on other consequences of sci, such as neuropathic pain and autonomic dysfunction. | 2009 | 19451618 |
| squamous cell carcinoma of the urinary bladder: a clinicopathologic and immunohistochemical study of 16 cases. | squamous cell carcinoma is an uncommon histologic type in the urinary bladder. we searched our surgical pathology files and identified 16 cases of squamous cell carcinoma of the urinary bladder. the mean age of patients was 65.4 years (range, 41-77 years). all patients received transurethral resection of the bladder, which demonstrated pure squamous cell carcinomas. seven patients had clinical evidence of tumor spreading out of the bladder (t4) and did not undergo radical cystectomy. the other 9 ... | 2009 | 19454359 |
| safety and efficacy of sugammadex for the reversal of rocuronium-induced neuromuscular blockade in cardiac patients undergoing noncardiac surgery. | the present randomized, safety-assessor blinded, placebo-controlled trial was designed to assess safety and efficacy of sugammadex, a novel selective relaxant-binding agent, in patients with underlying cardiovascular disease undergoing noncardiac surgery. | 2009 | 19455040 |
| assessment of urinary iodine excretion among normal kuwaiti adults. | this study was performed to investigate the status of iodine intake among the kuwaiti population and its effect on thyroid function. the study group was comprised of 139 females and 86 males with a mean age of 33 and 35 years, respectively. urinary iodine excretion (uie) and serum free t4 (ft4), thyrotropin hormone (tsh), antiperoxidase antibodies (anti- tpoab), and antithyroglobulin antibodies (anti-tgab) were determined. median uie was 148 μg/l (within the recommended level by the world health ... | 2009 | 19455284 |
| thyroid status can influence brain mast cell population. | neuroanatomical mapping as well as the influence of thyroid status on brain mast cell distribution and detectable mast cell number in adult rana esculenta is studied. treatment with tizoxin (t4) does not modify number or activational state of brain mast cells, whereas administration of the antithyroid agent 6-n-propyl-2-thiouracil induces a significant increase (up to 40%) in the mast cell number within the telencephalon and diencephalon. hypophysectomy induces a significant decrease (up to 65%) ... | 2009 | 19456362 |
| anti-thyroid hormone activity of bisphenol a, tetrabromobisphenol a and tetrachlorobisphenol a in an improved reporter gene assay. | previously, we transiently transfected gal4-fused thyroid hormone receptor (tr) expressing vector and the gal4 response reporter structure puas-tk-luc into hepg2 cell, developed a tr beta-1 mediated reporter gene assay to screen for compounds that acted on the tr signaling pathway. in this study, we improved the test efficiency by changing the transfected cell line into cv-1 cell. triiodothyronine (t3) and thyroxine (t4) induced higher luciferase expression, with the median effective concentrati ... | 2009 | 19457453 |
| glycogenic induction of thyroid hormone conversion and leptin system activation in the liver of postpartum dairy cows. | in the regulation of energy metabolism, the liver plays an important role in the reinforcement of energy production. in periparturient cows the energy homeostasis turns into a negative energy balance that may shift the physiological regulation of energy balance towards pathological processes. propylene glycol (pg), as a complementary source of energy used in the nutrition of dairy cows, alters systemic thyroid hormone economy; however, the exact mechanism through which highly glycogenic feed sup ... | 2009 | 19457782 |
| [rapid site-directed mutagenesis on full-length plasmid dna by using designed restriction enzyme assisted mutagenesis]. | to use the designed restriction enzyme assisted mutagenesis technique to perform rapid site-directed mutagenesis on double-stranded plasmid dna. the target amino acid sequence was reversely translated into dna sequences with degenerate codons, resulting in large amount of silently mutated sequences containing various restriction endonucleases (res). certain mutated sequence with an appropriate re was selected as the target dna sequence for designing mutation primers. the full-length plasmid dna ... | 2009 | 19459340 |
| changes in the content of major proteins and selected hormones in the blood serum of piglets during the early postnatal period. | the aim of the study was to determine the changes in the content of major proteins, glucose and selected hormones in the blood of piglets during the first 7 days of neonatal life. the study involved an entire litter of eight newborn piglets of f1 hybrids (polish white large x polish landrace) delivered from one sow in the second gestation. in blood samples collected directly after parturition (before colostrum intake), in the 12th, 24th and 48th hour and in 7th day of life, the content of total ... | 2009 | 19459467 |