the diameter of anodic tio(2) nanotubes affects bone formation and correlates with the bone morphogenetic protein-2 expression in vivo. | introduction: recently, it has been demonstrated that the nanoscale environment is a critical factor for cellular behaviour. it has been shown that the diameter of tio(2) nanotube layers controls the cellular behaviour of cells involved in the bone-forming process in vitro. therefore, the aim of the present study was to investigate the effects of the diameter of tio(2) nanotubes on peri-implant bone formation and the expression of bone matrix proteins in vivo. materials and methods: ninety exper ... | 2011 | 21443609 |
[standardized selection and its consequences for genetic population structure (examplified by sus scrofa l.)]. | type of selection for total fitness, specific for domestic animals and termed by us standardizing selection, is discussed. consequences of such selection for the population structure genetic are considered taking a population of domestic pigs as an example. examples of effective and ineffective standardizing selection are presented. specific features of genetic determination often quantitative traits detected in analysis of standardizing selection are discussed. | 2011 | 21446188 |
combined chemical and optical methods for monitoring the early decay stages of surrogate human models. | as the body decays shortly after death, a variety of gases and volatile organic compounds (vocs) constantly emanate. ethical and practical reasons limit the use of human corpses in controlled, time-dependent, intervening experiments for monitoring the chemistry of body decay. therefore the utilization of pig carcasses serves as a potential surrogate to human models. the aim of this work was to study buried body decay in conditions of entrapment in collapsed buildings. six domestic pigs were used ... | 2011 | 21450424 |
species-specific variation in rela underlies differences in nf-?b activity: a potential role in african swine fever pathogenesis. | african swine fever virus (asfv) is a highly infectious disease of domestic pigs, with virulent isolates causing a rapidly fatal hemorrhagic fever. in contrast, the porcine species endogenous to africa tolerate infection. the ability of the virus to persist in one host while killing another genetically related host implies that disease severity may be, in part, modulated by host genetic variation. to complement transcription profiling approaches to identify the underlying genetic variation in th ... | 2011 | 21450812 |
quantitative computed tomography in porcine lung injury with variable versus conventional ventilation: recruitment and surfactant replacement. | biologically variable ventilation improves lung function in acute respiratory distress models. if enhanced recruitment is responsible for these results, then biologically variable ventilation might promote distribution of exogenous surfactant to nonaerated areas. our objectives were to confirm model predictions of enhanced recruitment with biologically variable ventilation using computed tomography and to determine whether surfactant replacement with biologically variable ventilation provides ad ... | 2011 | 21460711 |
vaccination with viral protein-mimicking peptides postpones mortality in domestic pigs infected by african swine fever virus. | periodic outbreaks of african swine fever virus (asfv) infection around the world threaten local populations of domestic pigs with lethal disease and provide grounds for pandemic spread. effective vaccination may bring this threat under control. we investigated the effectiveness of select peptides mimicking viral proteins in establishing a protective immune response. forty-six synthetic peptides based on the analysis of the complete nucleotide sequence of asfv were tested for immunogenicity in m ... | 2011 | 21468582 |
orthodontic tooth movement into jaw regions treated with synthetic bone substitute. | orthodontic tooth movement is fraught with risks and complications. root resorption is the most frequent and important outcome which may arise due to inappropriate force magnitude or tooth movement into dense or altered bone. this study was aimed to demonstrate histologic changes in tooth roots following movement into a jaw region treated with synthetic bone substitute. another objective was to evaluate the method of experimental tooth movement using an animal model. | 2010 | 21469267 |
a novel isolation method of brucella species and molecular tracking of brucella suis biovar 2 in domestic and wild animals. | brucella suis biovar 2 is the most common aetiological agent of porcine brucellosis in europe. b. suis biovar 2 is considered to have low zoonotic potential, but is a causative agent of reproductive losses in pigs, and it is thus economically important. the multilocus variable-number of tandem repeats genotyping analysis of 16 loci (mlva-16) has proven to be highly discriminatory and is the most suitable assay for simultaneously identifying b. suis and tracking infections. the aim of this study ... | 2011 | 21474256 |
xenobiotic metabolizing cytochrome p450 in pig, a promising animal model. | the pig has been used as an important animal model for human studies because of its similarity in size, physiology and disease development. however, in contrast to the extensive data available on the cytochrome p450 (cyp) system for humans and rodents, the data related to pig are limited because of, among others, the presence of intra-species differences (domestic pigs and minipigs). the knowledge of the cyp superfamily in a given experimental animal is crucial for pharmacological and toxicologi ... | 2011 | 21476973 |
voiding prior to parachuting. | multiple incidents of isolated bladder ruptures have occurred in the military parachuting community after jumps. the aim of this article is to demonstrate the amount of energy it takes to rupture a distended bladder and encourage prejump voiding. | 2011 | 21481808 |
variable effects of high-dose adrenaline relative to standard-dose adrenaline on resuscitation outcomes according to cardiac arrest duration. | adjustment of adrenaline (epinephrine) dosage according to cardiac arrest (ca) duration, rather than administering the same dose, may theoretically improve resuscitation outcomes. we evaluated variable effects of high-dose adrenaline (hda) relative to standard-dose adrenaline (sda) on resuscitation outcomes according to ca duration. | 2011 | 21482013 |
genome-wide footprints of pig domestication and selection revealed through massive parallel sequencing of pooled dna. | background: artificial selection has caused rapid evolution in domesticated species. the identification of selection footprints across domesticated genomes can contribute to uncover the genetic basis of phenotypic diversity. methodology/main findings: genome wide footprints of pig domestication and selection were identified using massive parallel sequencing of pooled reduced representation libraries (rrl) representing ∼2% of the genome from wild boar and four domestic pig breeds (large white, la ... | 2011 | 21483733 |
ileorectal bypass performed entirely through a transanal route in a porcine model. | background: natural orifice transluminal endoscopic surgery (notes), a new frontier of minimally invasive surgery, uses the body's natural orifices to create an access for surgical procedures. this study aimed to verify the technical feasibility of ileorectal bypass performed entirely through a transanal access. methods: the procedure was performed on 10 domestic pigs, after which they were killed. a transanal endoscopic microsurgery (tem) device and endoscopic and laparoscopic instruments were ... | 2011 | 21484531 |
partial protection against classical swine fever virus elicited by dendrimeric vaccine-candidate peptides in domestic pigs. | we report the immunogenicity of three dendrimeric peptide vaccine candidates for classical swine fever virus (csfv). each dendrimeric construct contained four copies of a b-cell epitope from the e2 glycoprotein of csfv [construct 1: e2 (694-712); 2: e2 (712-727); 3: e2 (829-842)] joined to a t-cell epitope from the ns3 protein (residues 1446-1460). intramuscular immunization of domestic pigs with the different constructs significantly reduced the clinical score after lethal challenge with csfv. ... | 2011 | 21496472 |
experimental pig model of old myocardial infarction with long survival leading to chronic left ventricular dysfunction and remodeling as evaluated by pet. | a pig model of reduced left ventricular (lv) function and remodeling or chronic heart failure with long survival after myocardial infarction (mi) has not been established. the aim of this study was to evaluate the pathophysiologic status of a pig model of old mi using a series of pet studies. | 2011 | 21498524 |
[extracorporeal cardiac shock wave therapy improved myocardial micro-vascular circulation after acute myocardial infarction at early stage in pigs]. | to explore the effect of low-energy extracorporeal shock wave therapy to improve myocardial micro-vascular circulation after acute myocardial infarction at the early stage in pig model. | 2011 | 21500558 |
characterization of a novel porcine enterovirus in domestic pig in hungary. | porcine enteroviruses (pevs) of genus enterovirus are small, non-enveloped viruses with single-stranded, positive sense genomic rna, belonging to the family picornaviridae. the discovery of two distinct serotypes (pev9 and 10) was first reported in 1979. despite the sporadic detection and partial genome sequences of these viruses our knowledge about the prevalence and molecular epidemiology of pev types in domestic pigs is very deficient. in this study, we identified a novel pev from fecal sampl ... | 2011 | 21504800 |
seroprevalence of toxoplasma gondii infection in domestic pigs in durango state, mexico. | abstract little is known concerning the prevalence of toxoplasma gondii infection in pigs in mexico. antibodies to t. gondii were determined in 1,077 domestic pigs in durango, mexico using the modified agglutination test (mat). two groups (a, b) of pigs were sampled: group a pigs (n=555) were raised in 3 geographical regions in durango state and group b pigs (n=522) were from sonora state but slaughtered in durango city. overall, antibodies to t. gondii were found in 137 (12.7%) of 1,077 pigs, w ... | 2011 | 21506843 |
hypothesis on the dual origin of the mammalian subplate. | the development of the mammalian neocortex relies heavily on subplate. the proportion of this cell population varies considerably in different mammalian species. subplate is almost undetectable in marsupials, forms a thin, but distinct layer in mouse and rat, a larger layer in carnivores and big-brained mammals as pig, and a highly developed embryonic structure in human and non-human primates. the evolutionary origin of subplate neurons is the subject of current debate. some hypothesize that sub ... | 2011 | 21519390 |
detection and phylogenetic characterization of human hepatitis e virus strains, czech republic. | to determine the origin of hepatitis e virus in the czech republic, we analyzed patient clinical samples. five isolates of genotypes 3e, 3f, and 3g were obtained. their genetic relatedness with czech strains from domestic pigs and wild boars and patient recollections suggest an autochthonous source likely linked to consumption of contaminated pork. | 2011 | 21529412 |
successful transfection of genes using aav-2/9 vector in swine coronary and peripheral arteries. | background: gene therapy has attracted attention for its potential to treat several cardiovascular diseases. the use of adeno-associated viral (aav) vectors to facilitate therapeutic gene transfer to suppress intimal hyperplasia is a promising concept. the objective of this study was to analyze the in vivo transduction of a novel recombinant aav-2/9 vector with sm22α promoter, containing β-galactosidase gene (lacz) or green fluorescent protein (gfp) as reporter genes, to the medial layer smooth ... | 2011 | 21529824 |
long-term evaluation of a selective retrograde coronary venous perfusion model in pigs (sus scrofa domestica). | the lack of suitable target vessels remains a challenge for aortocoronary bypass grafting in end-stage coronary heart disease. this study aimed to investigate the arterialization of cardiac veins as an alternative myocardial revascularization strategy in an experimental long-term model in pigs. selective retrograde perfusion of a coronary vein (aorta to coronary vein bypass, retrobypass) before ligation of the ramus interventricularis paraconalis (equivalent to the left anterior descending arter ... | 2011 | 21535926 |
pcr-based molecular characterization and insilico analysis of food-borne trematode parasites paragonimus westermani, fasciolopsis buski and fasciola gigantica from northeast india using its2 rdna. | food-borne fluke infections/trematodiases are emerging as a major public health problem worldwide with over 40 million people affected and over 10% of world population at risk of infection. the major concentration of these infections is in southeast asian and western pacific regions, where the epidemiological factors (including the prevalent socio-cultural food habits) are conducive for transmission of these infections. the preponderance of these infections is usually in food deficit poor commun ... | 2011 | 21544167 |
evaluation of post-farm-gate passive surveillance in swine for the detection of foot and mouth disease in australia. | pigs are considered high risk for the introduction and spread of foot and mouth disease (fmd) in australia. one of the most likely pathways of introduction of fmd into australia would be through the illegal importation of fmd-contaminated meat, which is then fed to feral or domestic pigs. locations where animals from different origins are commingled, such as livestock markets and abattoirs, pose a risk for disease spread. early detection of exotic diseases at these locations is crucial in limiti ... | 2011 | 21546105 |
protection of european domestic pigs from virulent african isolates of african swine fever virus by experimental immunisation. | african swine fever (asf) is an acute haemorrhagic disease of domestic pigs for which there is currently no vaccine. we showed that experimental immunisation of pigs with the non-virulent ourt88/3 genotype i isolate from portugal followed by the closely related virulent ourt88/1 genotype i isolate could confer protection against challenge with virulent isolates from africa including the genotype i benin 97/1 isolate and genotype x uganda 1965 isolate. this immunisation strategy protected most pi ... | 2011 | 21549789 |
effects of sustained abdominal aorta compression on coronary perfusion pressures and restoration of spontaneous circulation during cardiopulmonary resuscitation in swine. | objectives: the present study was undertaken to explore whether sustained abdominal aorta compression-cardiopulmonary resuscitation (saac-cpr), as a means, can raise coronary perfusion pressure (cpp) as well as restoration of spontaneous circulation (rosc) during cpr. in the present study, we hypothesised that saac-cpr elevates cpp during cpr and improves rosc, without causing liver laceration. methods: animals were randomised into one of two groups (standard cpr and saac-cpr). ten domestic swin ... | 2011 | 21550162 |
integration between a percutaneous implant and the porcine small bowel. | inflammatory bowel diseases, cancer or trauma may require removal of all or part of the intestines, leaving the patient with a need to wear external stoma appliances for collection of bowel contents. by connecting the small bowel to a percutaneous port, equipped with a sealing lid, a fully continent and leak proof stoma can be created without a need for permanently wearing stoma appliance. the prerequisites for a connection between a permanent, transabdominal implant and a visceral organ are lar ... | 2011 | 21563306 |
genotyping of mycoplasma hyopneumoniae in wild boar lung samples. | mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia (ep), an important cause of disease-associated losses in swine production and a role of wild boar in recurrent infections can be supposed. genotypes of m. hyopneumoniae from wild boar are unknown but could indicate its role as a potential reservoir. therefore, 34 lung samples being pcr-positive for m. hyopneumoniae from wild boar from the geneva region in switzerland were assayed by genotyping using the p146 and multi-locus se ... | 2011 | 21571452 |
thermodilution-derived indices for assessment of left and right ventricular cardiac function in normal and impaired cardiac function. | objective:: the aim of this study was to assess whether thermodilution-derived parameters of right and left ventricular cardiac function (right ventricular ejection fraction, global ejection fraction, cardiac function index) are able to track changes of cardiac contractile function and whether they are influenced by substantial preload reduction. design:: prospective animal study. setting:: university-affiliated animal research laboratory. subjects:: domestic pigs. interventions:: sixteen domest ... | 2011 | 21572331 |
emergence of classical swine fever virus in israel in 2009. | classical swine fever (csf) re-emerged in israel in february 2009 after an absence of 62years. the outbreak occurred on a domestic pig farm in northern israel and affected domestic pigs and wild boar. on the basis of phylogenetic analysis of a 190 base pair fragment of the e2 glycoprotein gene, the israeli csf virus strain belonged to genotype 2.1 and was genetically most similar to a chinese csf virus strain. | 2011 | 21592830 |
laparoscopic fixation of biologic mesh at the hiatus with fibrin or polyethylene glycol sealant in a porcine model. | background: the objective of this study was to determine the acute and chronic fixation strengths achieved by fibrin or polyethylene glycol (peg) sealants to secure biologic mesh at the esophageal hiatus in a porcine model. methods: for this study, 32 female domestic pigs were divided into four groups of 8 each. the four groups respectively received acute fibrin sealant, acute peg sealant, chronic fibrin sealant, and chronic peg sealant. laparoscopically, a 5.5 × 8.5-cm piece of biodesign surgis ... | 2011 | 21594740 |
quantitative assessment of cardiac output and left ventricular function by noninvasive phase-contrast and cine mri: validation study with invasive pressure-volume loop analysis in a swine model. | purpose: to validate noninvasive cardiac output measurements of phase-contrast magnetic resonance imaging (pc-mri) and cine mri using an invasive pressure-volume (pv) loop technique on a swine model. materials and methods: we compared three methods for evaluating cardiac function at rest and under pharmaceutical low-dose inotropic infusion conditions: 1) phase-contrast mri, 2) cine mri, and 3) pv loop relationship. these measurements were made in 14 domestic pigs under rest conditions. identical ... | 2011 | 21598342 |
[artyfechinostomum sufratyfex lane, 1915 (trematoda:echinostomatidae), a parasite of mammals in southern vietnam]. | trematoda artyfechinostomumn sufratyfex lane, 1915 was found in different mammal species of southern vietnam. original measurements of this species from domestic pig, black rat, and asian palm civet are given. | 2011 | 21598669 |
evolution of endogenous retroviruses in the suidae: evidence for different viral subpopulations in african and eurasian host species. | abstract: background: porcine endogenous retroviruses (pervs) represent remnants of an exogenous form that have become integrated in the domestic pig (sus scrofa) genome. although they are usually inactive, the capacity of gamma1 ervs to infect human cells in vitro has raised concerns about xenotransplantation because the viruses could cross the species barrier to humans. here we have analyzed the evolution of gamma1 ervs in ten species of suidae (suids, pigs and hogs) from eurasia and africa us ... | 2011 | 21609472 |
parenteral aluminum induces liver injury in a newborn piglet model. | parenteral nutrition-associated cholestasis remains a significant problem, especially for the surgical neonate. aluminum is a toxic element known to contaminate parenteral nutrition. we hypothesize that parenterally administered aluminum causes liver injury similar to that seen in parenteral nutrition-associated cholestasis. | 2011 | 21616246 |
the consumption of glucose during ex vivo lung perfusion correlates with lung edema. | ex vivo lung perfusion (evlp) has been recently proposed to recondition organs before transplantation from donors with marginal or unacceptable features. the aim of our investigation was to explore glucose consumption during evlp. | 2011 | 21620034 |
long-term treatment with nifedipine suppresses coronary hyperconstricting responses and inflammatory changes induced by paclitaxel-eluting stent in pigs in vivo: possible involvement of rho-kinase pathway. | aims accumulating evidence indicates that coronary vasoconstricting responses are enhanced at the edges of coronary segment implanted with a drug-eluting stent (des) compared with a bare-metal stent (bms) in humans. we have recently demonstrated that rho-kinase pathway plays an important role in des-induced coronary hyperconstricting responses associated with inflammatory changes in pigs in vivo. this study examined whether long-term treatment with calcium channel blocker suppresses des-induced ... | 2011 | 21624903 |
a comparative assessment of zootherapeutic remedies from selected areas in albania, italy, spain and nepal. | zootherapy is the treatment of human ailments with remedies derived from animals and their products. despite its prevalence in traditional medical practices worldwide, research on this phenomenon has often been neglected in comparison to medicinal plant research. interviews regarding zootherapeutic traditions were conducted with informants from albania, italy, nepal and spain. we identified 80 species used in zootherapeutic remedies, representing 4 phyla in the animal kingdom: annelida, arthropo ... | 2010 | 21625340 |
a prospective randomized comparison of traditional laparoendoscopic single-site surgery with needlescopic-assisted laparoscopic nephrectomy in the porcine model. | abstract background and purpose: laparoendoscopic single-site (less) surgery produces virtually no scar but is technically challenging because of the loss of triangulation. the objective of this study is to compare classic transumbilical less nephrectomy with needlescopic-assisted laparoscopy (nal) surgery. in doing so, we evaluated whether the addition of a single 2-mm subcostal port could restore triangulation while not jeopardizing recovery or cosmetic outcome in the porcine model. materials ... | 2011 | 21631303 |
treatment of major vein injury with the hemostatic fleece tachosil by interposing a peritoneal patch to avoid vein thrombosis: a feasibility study in pigs. | vein lacerations in awkward locations are difficult to repair and carry high mortality. the hemostatic fleece, tachosil, is effective in preventing intraoperative bleeding in different settings, but has not been recommended for use in large vein injury. tachosil with a peritoneal patch interposed to avoid vein thrombosis has been reported as a method to obtain hemostasis in vein laceration, but further studies of this method are needed. | 2011 | 21633573 |
[laparoscopic pancreatic resections in experimental setting and clinical practice]. | pancreatic fistula is a major postoperative complication after pancreatic resection. one of the main risk factors of developing the pancreatic fistula after distal pancreatectomy is the method employed for the management of the pancreatic remnant. | 2011 | 21634100 |
differential resistance of mammalian sperm chromatin to oxidative stress as assessed by a two-tailed comet assay. | protamines of eutherian species are cysteine-rich molecules that become cross-linked by disulfide bonds during epididymal transit, whereas the protamines of most marsupial species lack cysteine residuals. the present study made use of the differences in protamine structure between eutherian and metatherian mammal spermatozoa to examine the comparative resistance of sperm dna to oxidative damage in three eutherian species (mus musculus, homo sapiens, sus domesticus) and three metatherian species ... | 2011 | 21635811 |
evaluation of genetic variability in the collared peccary pecari tajacu and the white-lipped peccary tayassu pecari by microsatellite markers. | in this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. six primers developed for domestic pigs were used and amplified in both species. they revealed the presence of five polymorphic loci and one monomorphic locus. the polymorphic loci included 4 of the 16 alleles in collared peccaries, and 3 of the 10 alleles in the white-lipped peccaries. polymorphic information content (pic) in both spec ... | 2010 | 21637606 |
genetic diversity and positive selection analysis of classical swine fever virus isolates in south china. | classical swine fever virus (csfv) causes a highly contagious disease that leads to significant economic losses in the pig industry worldwide. however, there is a paucity of knowledge on the accurate genotyping of csfv isolates in south china. this study genotyped the e2 gene of 14 csfv strains isolated during 2008-2010 from domestic pigs in different districts of south china. phylogenetic analyses revealed that all of the 14 csfv isolates were clustered into genetic subgroup 1.1. this contrasts ... | 2011 | 21643769 |
the first report of mycobacterium celatum isolation from domestic pig (sus scrofa domestica) and roe deer (capreolus capreolus) and an overview of human infections in slovenia. | mycobacterium celatum, a slowly growing potentially pathogenic mycobacterium first described in humans, is regarded as an uncommon cause of human infection, though capable of inducing invasive disease in immunocompromised hosts. according to some reports, a serious disease due to m. celatum may also occur in individuals with no apparent immunodeficiency. in animals, an m. celatum-related disease has been described in three cases only: twice in a domestic ferret (mustela putorius furo) and once i ... | 2011 | 21647336 |
outbreak of swine erysipelas in a semi-intensive wild boar farm in spain. | swine erysipelas (se) is a disease caused by the bacterium erysipelothrix rhusiopathiae and is one of the best-known and most serious diseases affecting domestic pigs. however, few studies exist concerning the susceptibility of wild boars to this disease and the role of this species as a reservoir. this study investigates and describes an outbreak of se that occurred on a semi-intensive wild boar breeding farm housing 40 boars in extremadura (sw spain) on 11-18 february 2010. seven animals died, ... | 2011 | 21649879 |
a pig model for the histomorphometric evaluation of hard tissue around dental implants. | abstract this study aimed to evaluate the frontal bone of swiss domestic pigs as an animal model for the histologic-histomorphometric examination of bony tissue around dental implants. sla surface implants 4.1 mm in diameter and 10 mm in length were inserted into the frontal bones of 9 swiss-domestic pigs. histologic and histomorphometric studies were conducted on the undecalcified sections. histological examinations showed that the specimens contain sufficient amount of bone, which provides hom ... | 2011 | 21651415 |
middle ear of human and pig: a comparison of structures and mechanics. | animal studies are an essential method to evaluate implants for the middle ear prior to clinical studies. new zealand rabbits, guinea pigs and chinchillas are, among other small mammals, well established animal models, but their auditory system is significantly smaller compared to human. hence, the suitability of the domestic pig (sus scrofa domesticus) as a new animal model for research on the middle ear (me), that would match the human in size, was investigated. | 2011 | 21657989 |
quality traits of wild boar mould-ripened salami manufactured with different selections of meat and fat tissue, and with and without bacterial starter cultures. | quality traits of wild boar mould-ripened salami were assessed in eight batches produced from two different assortments of meat (hind leg vs. shoulder), fat tissue (backfat from domestic pigs vs. fat tissue from wild boar), and with and without addition of a commercial bacterial starter culture. chemical composition of finished products (day 35) were in compliance with national food codex. batches produced with a bacterial starter culture were generally preferred by taste panels, had significant ... | 2011 | 21664055 |
heart-lung interactions measured by electrical impedance tomography. | : the clinical value of stroke volume variations to assess intravascular fluid status in critically ill patients is well known. electrical impedance tomography is a noninvasive monitoring technology that has been primarily used to assess ventilation. we investigated the potential of electrical impedance tomography to measure left ventricular stroke volume variation as an expression of heart-lung interactions. the objective of this study was thus to determine in a set of different hemodynamic con ... | 2011 | 21666450 |
transmission of salmonella between swine farms by the housefly (musca domestica). | the domestic pig is an important source of human salmonellosis, and houseflies are potential mechanical vectors of foodborne salmonella pathogens. in 2005, we recovered 144 salmonella isolates from flies and swine stool samples from 11 farms in taoyuan county and hsin chu county (northwestern taiwan). a total of 71.5% of the isolates were resistant to at least three antibiotics. there were a total of 14 serotypes, and 8 of these serotypes were present in both flies and swine stool samples. some ... | 2011 | 21669083 |
measurement of the midpalatal suture width. | abstract objective: to conduct a pilot study to investigate the potentiality to determine the midpalatal sutural width radiographically with a flat-panel volume computed tomography (fpvct) in a porcine model. materials and methods: bone samples from the midpalatal suture of five young (16 weeks) and five old (200 weeks) sus scrofa domestica were gathered. the midpalatal suture width was measured via fpvct and compared to respective histological preparations. results with p < .05 were considered ... | 2011 | 21812573 |
systemic gene delivery in large species for targeting spinal cord, brain, and peripheral tissues for pediatric disorders. | adeno-associated virus type 9 (aav9) is a powerful tool for delivering genes throughout the central nervous system (cns) following intravenous injection. preclinical results in pediatric models of spinal muscular atrophy (sma) and lysosomal storage disorders provide a compelling case for advancing aav9 to the clinic. an important translational step is to demonstrate efficient cns targeting in large animals at various ages. in the present study, we tested systemically injected aav9 in cynomolgus ... | 2011 | 21811247 |
hepatitis e virus in domestic pigs and surface waters in slovenia: prevalence and molecular characterization of a novel genotype 3 lineage. | a novel hepatitis e virus (hev) genotype 3 lineage was identified in slovenian pig herds. stool samples from six slovenian pig farms were collected and tested for the presence of hev rna. of 85 individual samples 15 (20.3%) were positive for hev rna of which 2/38 (5.3%), 6/21 (28.6%) and 7/26 (26.9%) were from suckling, weanling and fattening pigs, respectively. additionally, 51 pooled porcine stool samples were tested in one of the biggest pig farm and the estimated infection rate of individual ... | 2011 | 21802527 |
the codon usage model of the context flanking each cleavage site in the polyprotein of foot-and-mouth disease virus. | to investigate the codon usage pattern of the contexts flanking 11 cleavage sites of foot-and-mouth disease virus (fmdv) polyprotein, the codon usage model of the corresponding codon position and the synonymous codon usage in the target contexts of 66 strains were characterized by two simple methods based on the relative synonymous codon usage value. the synonymous codons usage pattern was also compared between this virus and two species of hosts (cattle and domestic pig). it is indicated that f ... | 2011 | 21801856 |
african swine fever virus p72 genotype ix in domestic pigs, congo, 2009. | african swine fever virus p72 genotype ix, associated with outbreaks in eastern africa, is cocirculating in the republic of the congo with west african genotype i. data suggest that viruses from eastern africa are moving into western africa, increasing the threat of outbreaks caused by novel viruses in this region. | 2011 | 21801650 |
connexin 43 gene therapy prevents persistent atrial fibrillation in a porcine model. | aims atrial fibrillation (af) is the most common sustained cardiac arrhythmia, and effective treatment of af still remains an unmet medical need. af is associated with atrial conduction disturbances caused by electrical and/or structural remodeling. we hypothesized that af suppresses expression of the gap junction protein connexin 43, and that cx43 gene transfer to both atria would prevent persistent atrial fibrillation. the first aim of this study was to assess whether atrial fibrillation (af) ... | 2011 | 21799069 |
catheter-based renal sympathetic denervation: chronic preclinical evidence for renal artery safety. | background: renal sympathetic hyperactivity is associated with hypertension, a leading cause of mortality worldwide. renal sympathetic denervation via the symplicity catheter system has been shown to decrease blood pressure by 33/11 mmhg by 6 months, with no radiofrequency (rf)-related adverse sequelae visible by ct/mr angiography or renal duplex ultrasound 6 months after the procedure. here, we present preclinical work predating those clinical results. we performed therapeutic renal sympathetic ... | 2011 | 21796327 |
comparison of anterior transgastric access techniques for natural orifice translumenal endoscopic surgery. | background: the advancement of natural orifice translumenal endoscopic surgery (notes) into clinical practice is dependent on its safety, efficacy, and efficiency. access is the obligatory first step in notes and serves as a surrogate to technical difficulties associated with this novel surgical approach. this study aimed to compare endoscopic transgastric access techniques in terms of safety, reproducibility, and efficiency. methods: seven variations for anterior transgastric notes access were ... | 2011 | 21789648 |
porcine kobuvirus from pig stool specimens in shanghai, china. | in this study, a total of 116 stool specimens were collected from pigs of different ages in three pig farms of shanghai in china during 2010. forty-five (38.8%) stool specimens were positive for porcine kobuvirus using reverse transcriptase-polymerase chain reaction (rt-pcr). the prevalence rate of porcine kobuvirus in the three pig farms which are located in minhang district, qingpu district and fengxian district was 46.7%(21/45), 35.1%(13/37), and 32.4%(11/34), respectively. we demonstrated th ... | 2011 | 21789634 |
influence of tongue inspection during cysticercosis diagnosis on some behavioral and physiological stress measures in pigs (sus scrofa domestica). | to evaluate the level of stress induced by the tongue inspection method during cysticercosis diagnosis in pigs at 60 and 90 kg, 27 animals were assigned to one of three experimental groups. in the first group (t1), pigs were held with a snout noose. in addition, animals in t2 were subjected to tongue inspection, while in t0, none of the procedures described above were performed. resistance to be handled and serum cortisol concentration were higher (p?<?0.05) than those from t0 in t1 and t2 group ... | 2011 | 21786048 |
suppression of persistent atrial fibrillation by genetic knockdown of caspase 3: a pre-clinical pilot study. | aims atrial fibrillation (af) is linked to cardiomyocyte apoptosis, leading to atrial remodelling and reduction in electrical conduction velocity. we hypothesized that genetic suppression of an apoptotic key enzyme, caspase 3, would prevent the development of persistent af by reducing apoptosis which may serve as an arrhythmogenic substrate. methods and results atrial fibrillation was induced in domestic pigs by atrial burst pacing via an implanted cardiac pacemaker. study animals were then assi ... | 2011 | 21785105 |
vertebral growth modulation by hemicircumferential electrocoagulation: an experimental study in pigs. | introduction: this experimental study in pigs was aimed at evaluating spinal growth disorders after partial arrest of the vertebral epiphyseal plates (ep) and neurocentral cartilages (ncc). unilateral and multisegmental single or combined lesions of the physeal structures were performed by electrocogulation throughout a video-assisted thoracoscopical approach. materials and methods: thirty 4-week-old domestic pigs (mean weight 16 kg) were included in the experiments. the superior and inferior ep ... | 2011 | 21779856 |
cardiac aav9-s100a1 gene therapy rescues post-ischemic heart failure in a preclinical large animal model. | as a prerequisite for clinical application, we determined the long-term therapeutic effectiveness and safety of adeno-associated virus (aav)-s100a1 gene therapy in a preclinical large animal model of heart failure. s100a1, a positive inotropic regulator of myocardial contractility, becomes depleted in failing cardiomyocytes in humans and animals, and myocardial-targeted s100a1 gene transfer rescues cardiac contractile function by restoring sarcoplasmic reticulum calcium (ca(2+)) handling in acut ... | 2011 | 21775667 |
mri studies of cryoinjury infarction in pig hearts: ii. effects of intrapericardial delivery of adipose-derived stem cells (adsc) embedded in agarose gel. | the purpose was to assess effects of intrapericardially deposited adipose-derived stem cells (adsc) as a source of angiogenic factors on cryoinjury infarction. to enhance this effect and reduce incorporation of adsc into tissue, the cells were immobilized in agarose gel patches transplanted onto cryoinjured epicardium. in domestic pigs (15-20?kg) the left ventricular (lv) anterior wall of exposed hearts was cryoinjured using aluminum rod (f=25?mm) cooled in liquid nitrogen. sterilized circular p ... | 2011 | 21774011 |
prevalence of hepatitis e virus-specific antibodies in humans with occupational exposure to pigs. | due to the increasing number of non-travel-associated hepatitis e virus (hev) infections observed in several industrialised countries including germany, there is a substantial interest in the characterisation of risk factors and transmission routes relevant to autochthonous hev infections. autochthonous cases are believed to be the result of a zoonotic hev transmission from pigs, wild boars and deer. recently, a high prevalence of hev-specific antibodies in the german domestic pig population has ... | 2011 | 21773797 |
molecular identification and phylogenetic analysis of trichinella isolates from different provinces in mainland china. | polymerase chain reaction (pcr) and sequencing are useful for species identification of trichinella spp., especially when their morphological characteristics useful for identifying taxa are lacking. in the present study, nine trichinella isolates from different provinces in mainland china were identified by the pcr-based method using the 5s ribosomal dna intergene spacer region (5s isr) and the mitochondrial large subunit ribosomal dna genes as molecular markers. the results indicated that eight ... | 2011 | 21773771 |
mitochondrial targeting of peroxiredoxin 5 is preserved from annelids to mammals but is absent in pig sus scrofa domesticus. | peroxiredoxin 5 (prdx5) is a thioredoxin peroxidase able to reduce hydrogen peroxide, alkyl hydroperoxides and peroxynitrite. in human, prdx5 was reported to be localized in the cytosol, the mitochondria, the peroxisomes and the nucleus. mitochondrial localization results from the presence of an n-terminal mitochondrial targeting sequence (mts). here, we examined the conservation of mitochondrial localization of prdx5 in animal species. we found that prdx5 mts is present and functional in the an ... | 2011 | 21763786 |
mesenchymal stem cells show radioresistance in vivo. | irradiation impacts on the viability and differentiation capacity of tissue-borne mesenchymal stem cells (msc), which play a pivotal role in bone regeneration. as a consequence of radiotherapy, bones may develop osteoradionecrosis. when irradiating human bone-derived msc in vitro with increasing doses, the cells' self-renewal capabilities were greatly reduced. mitotically stalled cells were still capable of differentiating into osteoblasts and preadipocytes. as a large animal model comparable to ... | 2011 | 21762375 |
determination of the mechanical properties of the skin of pig foetuses with respect to its structure. | skin is an important barrier protecting the organism against external environmental factors. determination of its mechanical characteristics as regards its structure has significant scientific and application value. in this work, uniaxial tensile tests were conducted to determine the basic mechanical parameters of skin with respect to its structure. the subject of the study were skin samples taken from domestic pig foetuses. they were excised from different parts of body, in the direction parall ... | 2011 | 21761809 |
sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets. | | 2011 | 21755265 |
mrna expression profiles of p450 3a enzymes in the liver and small intestine of the domestic pig. | the cytochrome p450 (p450) 3a family is considered to be the most important and abundantly expressed p450 subfamily in mammals. the mrna expression levels of four p450 3a enzymes in porcine liver and small intestine were investigated using real-time reverse transcriptase-polymerase chain reaction (rt-pcr). the expression of p450 3a mrnas (p450 3a pool) was higher in the liver than that in the small intestine. in the small intestine, the p450 3a mrnas were gradually decreased from the duodenum to ... | 2011 | 21752411 |
diabetes mellitus negatively affects peri-implant bone formation in the diabetic domestic pig. | von wilmowsky c, stockmann p, harsch i, amann k, metzler p, lutz r, moest t, neukam fw, schlegel ka: diabetes mellitus negatively affects peri-implant bone formation in the diabetic domestic pig. j clin periodontol 2011; 38: 771-779. doi: 10.1111/j.1600-051x.2011.01746.x. abstract: aim: diabetes mellitus is classified as a relative contraindication for implant treatment, and higher failure rates have been seen in diabetic patients. the aim of the present study was to investigate the effect of di ... | 2011 | 21752046 |
feasibility of initiating extracorporeal life support during mechanical chest compression cpr: a porcine pilot study. | background: recently, portable extracorporeal membrane oxygenation (ecmo) machines have become commercially available. this creates the potential to utilize extracorporeal life support (ecls) for the treatment of sudden cardiac arrest in the emergency department, and potentially in the out-of-hospital setting. objective: we sought to determine the feasibility of installing the ecmo circuit during delivery of mechanical chest compression cpr. methods: we used 5 mixed-breed domestic swine with a m ... | 2011 | 21835144 |
epidemiological and clinical aspects of human brucella suis infection in polynesia. | summaryhigh brucellosis seroprevalence rates in domestic swine herds have been reported in wallis and futuna islands and are associated with a significant burden of human infection by brucella suis, a species that is rarely incriminated in human disease. between 2003 and 2010, seven patients had a positive blood culture for b. suis biovar 1, 11 symptomatic patients had a positive rose bengal test (rbt) and a positive serum agglutination test (sat) and three asymptomatic cases were found to be po ... | 2011 | 21733252 |
sequencing technologies and genome sequencing. | the high-throughput - next generation sequencing (ht-ngs) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the sanger method. with the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cos ... | 2011 | 21698376 |
sensitivity and ex vivo validation of finite element models of the domestic pig cranium. | a finite element (fe) validation and sensitivity study was undertaken on a modern domestic pig cranium. bone strain data were collected ex vivo from strain gauges, and compared with results from specimen-specific fe models. an isotropic, homogeneous model was created, then input parameters were altered to investigate model sensitivity. heterogeneous, isotropic models investigated the effects of a constant-thickness, stiffer outer layer (representing cortical bone) atop a more compliant interior ... | 2011 | 21718316 |
seroprevalence of taenia solium infections in croatian patients presenting with epilepsy. | epilepsy is one of the most common neurological disorders, while neurocysticercosis caused by taenia solium infection of the central nervous system currently represents the leading cause of secondary epilepsy in central and south america, east and south asia, and sub-saharan africa. as a result of increased migration from these endemic regions, neurocysticercosis and subsequent epilepsy are becoming a growing public health problem in developed countries as well. in order to determine the prevale ... | 2011 | 21729385 |
pluripotent stem cells and reprogrammed cells in farm animals. | pluripotent cells are unique because of their ability to differentiate into the cell lineages forming the entire organism. true pluripotent stem cells with germ line contribution have been reported for mice and rats. human pluripotent cells share numerous features of pluripotentiality, but confirmation of their in vivo capacity for germ line contribution is impossible due to ethical and legal restrictions. progress toward derivation of embryonic stem cells from domestic species has been made, bu ... | 2011 | 21682936 |
genetic characterization and phylogeography of the wild boar sus scrofa introduced into uruguay. | the european wild boar sus scrofa was first introduced into uruguay, in southern south america during the early decades of the last century. subsequently, and starting from founder populations, its range spread throughout the country and into the neighbouring brazilian state rio grande do sul. due to the subsequent negative impact, it was officially declared a national pest. the main aim in the present study was to provide a more comprehensive scenario of wild boar differentiation in uruguay, by ... | 2011 | 21734838 |
impact of epididymal maturation, ejaculation and in vitro capacitation on tyrosine phosphorylation patterns exhibited of boar (sus domesticus) spermatozoa. | mammalian spermatozoa acquire functionality during epididymal maturation and ability to penetrate and fertilize the oocyte during capacitation. the aim of this study was to investigate the impact of epididymal maturation, ejaculation and capacitation on phosphotyrosine content of sperm proteins. western blot, immunocytochemical and flow cytometry analyses demonstrated that epididymal maturation in vivo is associated with a progressive loss of phosphotyrosine residues of the sperm head followed b ... | 2011 | 21835448 |
hemodynamic and histologic characterization of a swine (sus scrofa domestica) model of chronic pulmonary arterial hypertension. | the purpose of this work was to develop and characterize an aortopulmonary shunt model of chronic pulmonary hypertension in swine and provide sequential hemodynamic, angiographic, and histologic data by using an experimental endoarterial biopsy catheter. nine yucatan female microswine (sus scrofa domestica) underwent surgical anastomosis of the left pulmonary artery to the descending aorta. sequential hemodynamic, angiographic, and pulmonary vascular samples were obtained. six pigs (mean weight, ... | 2011 | 21819696 |
gouleako virus isolated from west african mosquitoes constitutes a proposed novel genus in the family bunyaviridae. | the family bunyaviridae is the most diversified family of rna viruses. we describe a novel prototypic bunyavirus, tentatively named goul+®ako virus, isolated from various mosquito species trapped in c+¦te d'ivoire. the s segment comprised 1,087 nucleotides (nt), the m segment 3,188 nt, and the l segment 6,358 nt, constituting the shortest bunyavirus genome known so far. the virus had shorter genome termini than phleboviruses and showed no evidence of encoded nss and nsm proteins. an uncharacteri ... | 2011 | 21715500 |
pathologic evaluation of hemostatic agents in percutaneous nephrolithotomy tracts in a porcine model. | abstract background and purpose: hemostatic agents have been suggested as an adjunct for tubeless percutaneous nephrolithotomy (pcnl). we pathologically evaluated the percutaneous tracts injected with the fibrin sealant (fs) evicel and hemostatic gelatin matrix (hgm) surgiflo at various time intervals to determine their absorption and tract closure rates. we also evaluated whether these agents reduced urine leak rates in a porcine model. materials and methods: percutaneous access was obtained in ... | 2011 | 21740197 |
genome engineering with zinc-finger nucleases. | zinc-finger nucleases (zfns) are targetable dna cleavage reagents that have been adopted as gene-targeting tools. zfn-induced double-strand breaks are subject to cellular dna repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. this article briefly reviews the history of zfn development and summarizes applications that have been made to genome editing in many different organisms and situations. considerable progress has been made i ... | 2011 | 21828278 |
current perspectives on the phylogeny of filoviridae. | sporadic fatal outbreaks of disease in humans and non-human primates caused by ebola or marburg viruses have driven research into the characterization of these viruses with the hopes of identifying host tropisms and potential reservoirs. such an understanding of the relatedness of newly discovered filoviruses may help to predict risk factors for outbreaks of hemorrhagic disease in humans and/or non-human primates. recent discoveries such as three distinct genotypes of reston ebolavirus, unexpect ... | 2011 | 21742058 |
selection of reference genes for gene expression studies in pbmc from bama miniature pig under heat stress. | heat stress decreases immune function and increases disease susceptibility in stressed animals, which are important factors for industry and public health. we investigated the molecular mechanisms underlying heat stress by profiling the expression of target genes involved in the cellular response in the blood of bama miniature pigs (sus scrofa domestica) over 21 days with the use of quantitative rt-pcr (qrt-pcr). reliable standards were established for the normalization of qrt-pcr. six potential ... | 2011 | 21820186 |
toxoplasma gondii in switzerland: a serosurvey based on meat juice analysis of slaughtered pigs, wild boar, sheep and cattle. | toxoplasmosis is one of the most important zoonotic diseases worldwide and is caused by the protozoan toxoplasma gondii. besides vertical infection during pregnancy, humans can get infected post-natally either by peroral uptake of sporulated toxoplasma oocysts or by ingestion of tissue cysts upon consumption of raw or undercooked meat. the aim of this study was to approximate the risk of human infection via meat consumption by estimating the seroprevalence of t. gondii in slaughtered animals in ... | 2011 | 21824348 |
enhanced delivery of topically-applied formulations following skin pre-treatment with a hand-applied, plastic microneedle array. | the purpose of this work is to characterize microchannels created by polymeric microneedles, applied by hand, and to demonstrate enhanced delivery of topically applied formulations of lidocaine hydrochloride and methylprednisolone sodium succinate (mpss). 3m's microstructured transdermal system (mts) arrays were applied to domestic swine to demonstrate reliability of penetration, depth of penetration and durability of the structures to repeat application and high force. tissue levels of lidocain ... | 2011 | 21696356 |
randomized objective comparison of live tissue training versus simulators for emergency procedures. | there is a lack of objective analysis comparing live tissue and simulator training. this article aims to objectively determine the difference in outcomes. twenty-four air force volunteers without prior experience performing emergency procedures were randomly assigned to receive training in tube thoracostomy (chest tube) and cricothyroidotomy training on either a pig model (sus scrofa domestica) or on the traumaman simulator. one week posttraining, students were tested on human cadavers with obje ... | 2011 | 21679588 |
neisseria meningitidis and escherichia coli are protected from leukocyte phagocytosis by binding to erythrocyte complement receptor 1 in human blood. | the initial interaction of gram-negative bacteria with erythrocytes and its implications on leukocyte phagocytosis and oxidative burst in human whole blood were examined. alexa-labeled escherichia coli, wild-type h44/76 n. meningitidis and the h44/76lpxa lipopolysaccharide (lps)-deficient mutant were incubated with whole blood using lepirudin as anticoagulant which has no adverse effects on complement. bacteria free in plasma, bound to erythrocytes or phagocytized by granulocytes and monocytes w ... | 2011 | 21839519 |
genetic detection and analysis of porcine bocavirus type 1 (pobov1) in european wild boar (sus scrofa). | novel porcine parvoviruses showing the genetic characteristics of bocaviruses have recently been identified. the first such porcine bocavirus (pobov1), described as boca-like virus (pbo-likev), was discovered in pmws affected pigs in sweden. later, several other bocaviruses with divergent genomes were reported under various names in domestic pigs. this is the first report of the presence of bocaviruses in european wild boars. 842 wild boar samples originating from the western region of romania ( ... | 2011 | 21822671 |
relative distribution of drugs in decomposed skeletal tissue. | skeletal tissues from a domestic pig exposed to amitriptyline, diazepam, and pentobarbital were analyzed to determine the relative distribution of these drugs in bone. following drug exposure and euthanasia, remains were allowed to decompose outdoors to complete skeletonization between summer 2007 and fall 2009. remains were recovered and separated according to bone type. twelve different bone types were pulverized and sampled in triplicate. each bone sample underwent methanolic extraction (96 h ... | 2010 | 21819797 |
development of lidocaine-coated microneedle product for rapid, safe, and prolonged local analgesic action. | purpose: to demonstrate rapid (~1-ámin) lidocaine delivery using 3m's solid microstructured transdermal system (smts) for prolonged, local analgesic action. methods: polymeric microneedles were fabricated via injection molding and then dip-coated using an aqueous lidocaine formulation. the amount of lidocaine coated onto the microneedles was determined by high performance liquid chromatography (hplc). to assess drug delivery and dermal pharmacokinetics, lidocaine-coated microneedles were inserte ... | 2011 | 21735335 |
pseudorabies virus in wild swine: a global perspective. | suid herpesvirus 1 (suhv1, syn. aujeszky's disease virus [adv] or pseudorabies virus [prv]), which belongs to the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus is the causative agent of aujeszky's disease (ad, pseudorabies), a notifiable disease, that causes substantial economic losses to the swine industry in countries, where ad is present. members of the family suidae (true pigs) are the only natural hosts for prv, although the virus can infect numerous other mammals ... | 2011 | 21837416 |
guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - a comparison of different tissue sources. | due to donor side morbidity and the absence of osteogenic properties in bone substitutes, there is a growing need for an alternative to traditional bone grafting within the scope of tissue engineering. this animal study was conducted to compare the in-ávivo osteogenic potential of adipose-derived (ad), periosteum-derived (pd) and bone marrow-derived (bm) mesenchymal stem/progenitor cells (msc). autologous mesenchymal stem/progenitor cells of named tissue origin were induced into osteogenic diffe ... | 2011 | 21723141 |
non-contiguous finished genome sequence of bacteroides coprosuis type strain (pc139). | bacteroides coprosuis whitehead et al. 2005 belongs to the genus bacteroides, which is a member of the family bacteroidaceae. members of the genus bacteroides in general are known as beneficial protectors of animal guts against pathogenic microorganisms, and as contributors to the degradation of complex molecules such as polysaccharides. b. coprosuis itself was isolated from a manure storage pit of a swine facility, but has not yet been found in an animal host. the species is of interest solely ... | 2011 | 21677860 |
influence of the halothane gene (hal) on pork quality in two commercial crossbreeds. | we evaluated the effect of the halothane (hal) gene on the quality of pork in domestic pigs. half-carcasses from two different commercial pig (sus domestica) crossbreeds were analyzed, 46 of which were homozygous dominant (hal(nn)) and 69 of which were heterozygous (hal(nn)) for the halothane gene. the measures included backfat thickness, lean meat percentage, carcass weight, ph 24 h after slaughtering, color, and drip loss; dna was extracted from the haunch muscle. swine with the hal(nn) genoty ... | 2011 | 21823098 |
seroepidemiologic survey for chlamydia suis in wild boar (sus scrofa) populations in italy. | we used serology to estimate the prevalence of exposure to chlamydiae in italian populations of wild boars (sus scrofa). sera from 173 hunter-killed wild boars harvested during the 2006-2009 hunting seasons in three italian regions were tested for antibodies to chlamydia suis, chlamydophila pecorum, chlamydophila abortus, and chlamydophila psittaci by the microimmunofluorescence test. antibody titers to chlamydiae ôëñ 1:32 were detected in 110 of the 173 samples tested (63.6%). specific reactivi ... | 2011 | 21719838 |
comparison of the compression anastomosis ring (endocar) with a circular stapled anastomosis in a porcine model. | purpose. the aim of this study was to compare characteristics of rectal compression and stapled anastomoses at multiple time points. methods. a total of 50 domestic pigs underwent a rectal anastomosis with a compression device or a circular stapler. they were sacrificed at zero-time, 2 days, 1 week, 1 month, and 3 months. burst and maximal tolerated pressure and sites of failure, internal diameters, and radiographic leak rates were assessed. desmosine (elastin) levels were determined. results. t ... | 2011 | 21742666 |