| antimicrobial activity of tulathromycin and 14 other antimicrobials against virulent rhodococcus equi in vitro. | this study determined the antimicrobial activity of tulathromycin against rhodococcus equi in vitro. ninety-eight virulent isolates of r. equi from equine clinical cases were examined, of which 20 isolates were macrolide resistant. a custom 96-well antimicrobial susceptibility testing plate was used, allowing 14 additional antimicrobials to be tested against r. equi. isolates were cultured with various concentrations of antimicrobials, and minimal inhibitory concentration (mic) values were deter ... | 2010 | 20957614 |
| manipulating the stereoselectivity of limonene epoxide hydrolase by directed evolution based on iterative saturation mutagenesis. | limonene epoxide hydrolase from rhodococcus erythropolis dcl 14 (leh) is known to be an exceptional epoxide hydrolase (eh) because it has an unusual secondary structure and catalyzes the hydrolysis of epoxides by a rare one-step mechanism in contrast to the usual two-step sequence. from a synthetic organic viewpoint it is unfortunate that leh shows acceptable stereoselectivity essentially only in the hydrolysis of the natural substrate limonene epoxide, which means that this eh cannot be exploit ... | 2010 | 20958062 |
| rhodococcus erythropolis septicaemia in a patient with acute lymphocytic leukaemia. | rhodococcus erythropolis rarely causes infection in humans. we report the second case of r. erythropolis septicaemia in a 7-year-old child. however, to our knowledge it is the first case in a patient with acute lymphocytic leukaemia who had been undergoing chemotherapy. the identification was performed using 16s rrna gene sequencing. even though r. erythropolis is rarely associated with human infections, it should be considered as a potential causative agent of bacteraemia, rather than overlooke ... | 2010 | 20965915 |
| starvation/stationary-phase survival of rhodococcus erythropolis sq1: a physiological and genetic analysis. | the adaptation of rhodocccus erythropolis sq1 to energy and carbon starvation was investigated in terms of both the capacity to survive starvation and the contribution of a nutrient-induced stationary phase to cross-protection to other types of environmental stress. it was found that r. erythropolis sq1 survives for at least 43 days in lb and distilled water, and 65 days in chemically defined medium (cdm) containing high (1%) or low (0.1%) glucose. furthermore, early stationary-phase r. erythrop ... | 2010 | 20967536 |
| analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry. | a sensitive and reliable high-performance liquid chromatographic method with tandem mass spectrometric detection has been developed and used for the determination of 2-methylthio-cytokinin derivatives produced by the phytopathogenic actinomycete rhodococcus fascians. the cultivation medium containing secreted cytokinins was concentrated and subjected to a solid-phase extraction (c18 and ion-exchange). the purified samples were further separated and analyzed by hplc-esi-ms/ms. this allowed to ach ... | 2010 | 20969996 |
| effect of alkyl hydroxybenzenes on the properties of dioxygenases. | the aim of the present work was to investigate the influence of alkylhydroxybenzenes (ahbs) and tyrosol, which belong to cell differentiation factors d(1) group of autoregulators on properties of biodegradation enzymes, catechol 1,2-dioxygenase (cat 1,2-do) and methylcatechol 1,2-dioxygenase (mcat 1,2-do) of rhodococcus opacus 6a. ahbs were found to have a greater effect on mcat 1,2-do than on cat 1,2-do. it was expressed by more pronounced changes in the activity of mcat 1,2-do with unsubstitut ... | 2010 | 20972919 |
| virulence genes and plasmid profiles in rhodococcus equi isolates from domestic pigs and wild boars (sus scrofa) in brazil. | the virulence genes and plasmid profiles of 23 rhodococcus equi isolates from 258 lymph nodes from domestic pigs (129 nodes with lesions and 129 without lesions) and 120 lymph nodes from slaughtered wild boars (60 nodes with lesions and 60 without) were characterized. r. equi was obtained from 19 lymph nodes of domestic pigs, 17 with, and two without lesions, and from four lymph nodes with lesions, from wild boars. the 23 isolates were tested for the presence of vapa and vapb genes, responsible ... | 2010 | 20980033 |
| emulsification potential of a newly isolated biosurfactant-producing bacterium, rhodococcus sp. strain ta6. | an indigenous biosurfactant producing bacterium, rhodococcus sp. strain ta6 was isolated from iranian oil contaminated soil using an efficient enrichment and screening method. during growth on sucrose and several hydrocarbon substrates as sole carbon source, the bacterium could produce biosurfactants. as a result of biosurfactant synthesis, the surface tension of the growth medium was reduced from 68mnm(-1) to values below 30mnm(-1). the biosurfactant was capable of forming stable emulsions with ... | 2010 | 21030223 |
| biodegradation of di-n-butyl phthalate by rhodococcus sp. jdc-11 and molecular detection of 3, 4-phthalate dioxygenase gene. | rhodococcus sp. jdc-11, capable of utilizing di-n-butyl phthalate (dbp) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16s rrna gene sequence analysis. the optimum ph, temperature, and agitation rate for dbp degradation by rhodococcus sp. jdc-11 was 8.0, 30 degrees c, and 175 rpm, respectively. in addition, the effect of glucose concentration on dbp degradation indicated that low concentration of glucose inhibited the degradation of dbp whi ... | 2010 | 21030830 |
| the suitability of selected methods of nucleic acid extraction for detecting rhodococcus equi dna in tracheobronchial wash fluid using pcr. | the aim of the present study was to compare the effectiveness of selected dna extraction methods for the detection of rhodococcus equi from tracheobronchial wash fluid by pcr. three methods of nucleic acid extraction were evaluated, based mainly on the activity of proteolytic enzymes. a commercial kit for isolation and purification of bacterial dna was also used in the study. in one procedure, an additional component, the cationic detergent ctab, was used. it has been found that the traditional ... | 2010 | 21033553 |
| expression and inducibility of endosulfan metabolizing gene in rhodococcus strain isolated from earthworm gut microflora for its application in bioremediation. | the metabolizing potential of a bacterial strain rhodococcus mtcc 6716, isolated from the gut of an indian earthworm (metaphire posthuma) was studied for endosulfan bioremediation. in the present work, the optimum conditions for the maximum growth, kinetic of endosulfan degradation, regression equation, half life and correlation coefficient were studied. endosulfan induced alterations in the expression of mrna and protein of specific endosulfan metabolizing marker gene (esd) was studied. maximum ... | 2010 | 21035330 |
| disseminated rhodococcus equi infection in dromedary camels (camelus dromedarius). | rhodococcus (r). equi, a recognized pathogen in horses, is emerging as a human opportunistic pathogen, especially in immunocompromized people. it affects also new world camelids, but there are no reports of r. equi infection in old world camelids yet. four cases of disseminated r. equi infection in adult breeding dromedaries occurred at one camel farm near dubai within 16 months of each other. at necropsy the lungs were diffusely consolidated with large caseous areas. histology revealed severe s ... | 2010 | 21035967 |
| a high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry. | nitrile-hydrolyzing enzymes (nitrilase or nitrile hydratase/amidase) have been widely used in the pharmaceutical industry for the production of carboxylic acids and their derivatives, and it is important to build a method for screening for nitrile-hydrolyzing enzymes. in this paper, a simple, rapid, and high-throughput screening method based on the ferric hydroxamate spectrophotometry has been proposed. to validate the accuracy of this screening strategy, the nitrilases from rhodococcus erythrop ... | 2010 | 21038095 |
| multiple-genome comparison reveals new loci for mycobacterium species identification. | to identify loci useful for species identification and to enhance our understanding of the population structure and genetic variability of the genus mycobacterium, we conducted a multiple-genome comparison of a total of 27 sequenced genomes in the suborder of corynebacterineae (18 from the mycobacterium genus, 7 from the corynebacterium genus, 1 each from the nocardia and rhodococcus genera). our study revealed 26 informative loci for species identification in mycobacterium. the sequences from t ... | 2010 | 21048007 |
| a simple enzymatic method for production of a wide variety of d-amino acids using l-amino acid oxidase from rhodococcus sp. aiu z-35-1. | a simple enzymatic method for production of a wide variety of d-amino acids was developed by kinetic resolution of dl-amino acids using l-amino acid oxidase (l-aao) with broad substrate specificity from rhodococcus sp. aiu z-35-1. the optimum ph of the l-aao reaction was classified into three groups depending on the l-amino acids as substrate, and their respective activities between ph 5.5 and 8.5 accounted for more than 60% of the optimum activity. the enzyme was stable in the range from ph 6.0 ... | 2010 | 21048866 |
| analysis of the composition and characteristics of culturable endophytic bacteria within subnival plants of the tianshan mountains, northwestern china. | this study first described the composition and characteristics of culturable endophytic bacteria isolated from wild alpine-subnival plant species growing under extreme environmental conditions (i.e., on the border of a glacier with frequently fluctuating and freezing temperatures, strong wind, and high ultraviolet radiation). using a cultivation-dependent approach and 16s rrna gene amplification techniques, 93 bacterial isolates showing different phenotypic properties were obtained from 20 diffe ... | 2011 | 21061126 |
| [growth peculiarities of hydrocarbon-oxidizing rhodococcus and pseudomonads dissociates in mono- and mixed cultures]. | growth of r-, s-, and m-dissociates of rhodococcus rubropertinctus in mixed culture with r-, s-, and m-dissociates of rhodococcus aeruginosa in comparison with rhodococcus monoculture cultivated on mineral nutrient medium with hexadecane has been studied. the amount of cells in the stationary growth phase has increased 10-15 times in comparison with the monoculture, and pseudomonads which dominated in population, in associations of m-dissociate of r. rubropertinctus with any dissociate of r. aer ... | 2010 | 21061599 |
| glycogenformation by rhodococcus species and the effect of inhibition of lipid biosynthesis on glycogen accumulation in rhodococcus opacus pd630. | members of the genus rhodococcus were investigated for their ability to produce glycogen during cultivation on gluconate or glucose. strains belonging to rhodococcus ruber, rhodococcus opacus, rhodococcus fascians, rhodococcus erythropolis and rhodococcus equi were able to produce glycogen up to 0.2–5.6% of cellular dry weight (cdw). the glycogen content varied from 0.8% to 3.2% of cdw in cells of r. opacus pd630, which is a well-known oleaginous bacterium, during the exponential growth phase, w ... | 2010 | 21069909 |
| a new acylamidase from rhodococcus erythropolis ta37 can hydrolyze n-substituted amides. | a new acylamidase was isolated from rhodococcus erythropolis ta37 and characterized. n-substituted acrylamides (isopropyl acrylamide, n,n-dimethyl-aminopropyl acrylamide, and methylene-bis-acrylamide), acid para-nitroanilides (4'-nitroacetanilide, gly-pna, ala-pna, leu-pna), and n-acetyl derivatives of glycine, alanine, and leucine are good substrates for this enzyme. aliphatic amides (acetamide, acrylamide, isobutyramide, n-butyramide, and valeramide) are also used as substrates but with less e ... | 2010 | 21073421 |
| subcellular components of probiotics kocuria sm1 and rhodococcus sm2 induce protective immunity in rainbow trout (oncorhynchus mykiss, walbaum) against vibrio anguillarum. | the efficacy of cellular components of probiotics kocuria sm1 and rhodococcus sm2 to protect rainbow trout (oncorhynchus mykiss, walbaum) against vibriosis was assessed. groups of fish (average weight = 10-15 g) were immunized intraperitoneally (i.p.) with 0.1 ml of subcellular materials, i.e., 0.2 ± 0.05 mg protein per fish, comprising extracellular proteins (ecps), cell wall proteins (cwps) and whole cell proteins (wcps) of sm1 and sm2, respectively, or with 0.1 ml of phosphate-buffered saline ... | 2010 | 21078398 |
| genomic and functional analyses of rhodococcus equi phages reqipepy6, reqipoco6, reqipine5, and reqidocb7. | the isolation and results of genomic and functional analyses of rhodococcus equi phages reqipepy6, reqidocb7, reqipine5, and reqipoco6 (hereafter referred to as pepy6, docb7, pine5, and poco6, respectively) are reported. two phages, pepy6 and poco6, more than 75% identical, exhibited genome organization and protein sequence likeness to lactococcus lactis phage 1706 and clostridial prophage elements. an unusually high fraction, 27%, of pepy6 and poco6 proteins were predicted to possess at least o ... | 2010 | 21097585 |
| mycolic acid-containing bacteria induce natural-product biosynthesis in streptomyces species. | natural products produced by microorganisms are important starting compounds for drug discovery. secondary metabolites, including antibiotics, have been isolated from different streptomyces species. the production of these metabolites depends on the culture conditions. therefore, the development of a new culture method can facilitate the discovery of new natural products. here, we show that mycolic acid-containing bacteria can influence the biosynthesis of cryptic natural products in streptomyce ... | 2010 | 21097597 |
| biodegradation of rdx and mnx with rhodococcus sp. strain dn22: new insights into the degradation pathway. | previously we demonstrated that rhodococcus sp. strain dn22 can degrade rdx (hexahydro-1,3,5-trinitro-1,3,5-triazine) aerobically via initial denitration. the present study describes the role of oxygen and water in the key denitration step leading to rdx decomposition using (18)o(2) and h(2)(18)o labeling experiments. we also investigated degradation of mnx (hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine) with dn22 under similar conditions. dn22 degraded rdx and mnx giving no(2)(-), no(3)(-), nd ... | 2010 | 21105645 |
| expression, purification and functional characterization of a recombinant 2,3-dihydroxybiphenyl-1,2-dioxygenase from rhodococcus rhodochrous. | a 2,3-dihydroxybiphenyl (2,3-dhbp) dioxygenase gene from a rhodococcus sp. strain, named rrbphci and involved in the degradation of polychlorinated biphenyls (pcbs), was synthesized. rrbphci was expressed in escherichia coli and its encoded enzyme was purified. sds-page analysis indicated that the size of the protein encoded by rrbphci was about 32 kda. the activity of the 2,3-dhbp dioxygenase was 82.8 u/mg when the substrate was 2,3-dhbp, with optimum ph 8.0 at 30°c, and optimum temperature was ... | 2010 | 21113668 |
| inaugural structure from the duf3349 superfamily of proteins, mycobacterium tuberculosis rv0543c. | the first structure for a member of the duf3349 (pf11829) family of proteins, rv0543c from mycobacterium tuberculosis, has been determined using nmr-based methods and some of its biophysical properties characterized. rv0543c is a 100 residue, 11.3 kda protein that both size exclusion chromatography and nmr spectroscopy show to be a monomer in solution. the structure of the protein consists of a bundle of five α-helices, α1 (m1-y16), α2 (p21-c33), α3 (s37-g52), α4 (g58-h65) and α5 (s72-g87), held ... | 2010 | 21144816 |
| directed evolution of an aminoalcohol dehydrogenase for efficient production of double chiral aminoalcohols. | the aminoalcohol dehydrogenase (aadh) of rhodococcus erythropolis mak154, which can be used as a catalyst for the stereoselective reduction of (s)-1-phenyl-1-keto-2-methylaminopropane to d-pseudoephedrine (dpe), is inhibited by the accumulation of dpe in the reaction mixture, limiting the yield of dpe. to improve this weak point of the enzyme, random mutations were introduced into aadh, and a mutant enzyme library was constructed. the mutant library was screened with a color detectable high-thro ... | 2010 | 21163696 |
| modulation of cytokine secretion and oxidative metabolism of innate immune effectors by rhodococcus biosurfactant. | the glycolipid biosurfactant complex from rhodococcus ruber iegm 231 had a stimulatory effect on the production of il-12, il-18, and reactive oxygen species by cells of the innate immunity. this effect depended on the composition of cell cultures and presence of lps. it was primarily observed in non-stimulated cultures. the glycolipid biosurfactant complex had little effect on il-10 secretion by monocytes and mononuclear cells. | 2010 | 21165433 |
| [spectrum of primary immunodeficiencies in a tertiary hospital over a period of 10 years]. | more than 200 primary immunodeficiencies (pid) have been described and about 60% present during childhood. early diagnosis and treatment have been shown to improve patient outcome. | 2010 | 21168377 |
| ward round-- non-resolving pleural effusion in a patient with hiv infection. | | 2009 | 21174934 |
| abundance and community of snow bacteria from three glaciers in the tibetan plateau. | bacterial abundance and diversity in snow of east rongbuk, laohugou and hailuogou glaciers on the tibetan plateau were investigated through epifluorescence microscope and denaturing gradient gel electrophoresis. cell abundance ranged from 4.0 x 10(3) to 290.2 x 10(3) cells/ml. the phylogenetic trees placed the 16s rrna sequences in four major groups: proteobacteria, firmicutes, actinobacteria and bacteroidetes. brevundimonas, flavobacterium, hymenobacter, bacillus, polaromonas, rhodoferax and st ... | 2010 | 21174974 |
| functional characterization of a gene cluster involved in gentisate catabolism in rhodococcus sp. strain ncimb 12038. | rhodococcus sp. strain ncimb 12038 utilizes naphthalene as a sole source of carbon and energy, and degrades naphthalene via salicylate and gentisate. to identify the genes involved in this pathway, we cloned and sequenced a 12-kb dna fragment containing a gentisate catabolic gene cluster. among the 13 complete open reading frames deduced from this fragment, three (narikl) have been shown to encode the enzymes involved in the reactions of gentisate catabolism. nari is gentisate 1,2-dioxygenase wh ... | 2010 | 21181154 |
| flux-based analysis of sulfur metabolism in desulfurizing strains of rhodococcus erythropolis. | rhodococcus erythropolis has been studied widely for potential applications in biodesulfurization. previous works have been largely experimental with an emphasis on the characterization and genetic engineering of desulfurizing strains for improved biocatalysis. a systems modeling approach that can complement these experimental efforts by providing useful insights into the complex interactions of desulfurization reactions with various other metabolic activities is absent in the literature. in thi ... | 2010 | 21182538 |
| microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media. | cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. among the microorganisms studied here, the yeasts (schwanniomyces occidentalis, torulopsis candida, candida tropicalis, candida lipolytica, candida maltosa, candida paralipolytica) and two representative bacteria (rhodococcus sp. and pseudomonas putida) produced exocellular structures compos ... | 2010 | 21182540 |
| analyses of both the alkb gene transcriptional start site and alkb promoter-inducing properties of rhodococcus sp. strain bcp1 grown on n-alkanes. | rhodococcus sp. strain bcp1, known for its capacity to grow on short-chain n-alkanes (c(2) to c(7)) and to cometabolize chlorinated solvents, was found to also utilize medium- and long-chain n-alkanes (c(12) to c(24)) as energy and carbon sources. to examine this feature in detail, a chromosomal region which includes the alkb gene cluster encoding a non-heme di-iron monooxygenase (alkb), two rubredoxins, and one rubredoxin reductase was cloned from the bcp1 genome. furthermore, the activity of t ... | 2010 | 21193665 |
| effects of location for collection of air samples on a farm and time of day of sample collection on airborne concentrations of virulent rhodococcus equi at two horse breeding farms. | to determine whether airborne concentrations of virulent rhodococcus equi at 2 horse breeding farms varied on the basis of location, time of day, and month. | 2011 | 21194338 |
| gene expression analysis of methylotrophic oxidoreductases involved in the oligotrophic growth of rhodococcus erythropolis n9t-4. | rhodococcus erythropolis n9t-4 shows extremely oligotrophic growth requiring atmospheric co₂ without any additional carbon or energy source. we performed a gene expression analysis of the oxidoreductases, which are involved in methanol metabolism, under various growth and induction conditions in n9t-4. a real-time pcr analysis revealed that the genes encoding nad-dependent formaldehyde dehydrogenase (nfadh) and n,n'-dimethyl-4-nitrosoaniline-dependent methanol dehydrogenase (mdh) were strongly e ... | 2011 | 21228466 |
| expression in escherichia coli of biphenyl 2,3-dioxygenase genes from a gram-positive polychlorinated biphenyl degrader, rhodococcus jostii rha1. | rhodococcus jostii rha1 is a polychlorinated biphenyl degrader. multi-component biphenyl 2,3-dioxygenase (bpha) genes of rha1 encode large and small subunits of oxygenase component and ferredoxin and reductase components. they did not express enzyme activity in escherichia coli. to obtain bpha activity in e. coli, hybrid bpha gene derivatives were constructed by replacing ferredoxin and/or reductase component genes of rha1 with those of pseudomonas pseudoalcaligenes kf707. the results obtained i ... | 2011 | 21228494 |
| permeabilization induced by lipid ii-targeting lantibiotic nisin and its effect on the bioconversion of vitamin d3 to 25-hydroxyvitamin d3 by rhodococcus erythropolis. | vitamin d3 (vd3) is a fat-soluble prohormone in mammals. vd3 is inert and must be activated by hydroxylation at the c-25 and c-1α positions to exert its biological activity. we recently accomplished the bioconversion of vd3 to 25(oh)vd3 with a recombinant strain of rhodococcus erythropolis and found that the permeability of vd3 into the cytoplasm may be the rate-limiting step of 25(oh)vd3 production (sallam et al., 2010). when the cells were treated with the lipid ii-targeting lantibiotic nisin, ... | 2011 | 21237135 |
| immunocytochemical detection of rhodococcus equi in tracheal washes of foals. | the aim of the present study was to develop an immunocytochemical procedure for the early detection and demonstration of rhodococcus equi in smears of tracheal aspirates taken from live foals in field conditions. tracheal wash samples were collected from thoroughbred foals, aged 1-5 months and located in studs around bursa and istanbul, turkey. some foals were suspected of having r. equi infection on the basis of clinical examination (n=56) and others were unaffected control animals (n=54). seru ... | 2011 | 21238974 |
| improved identification of gordonia, rhodococcus and tsukamurella species by 5'-end 16s rrna gene sequencing. | the identification of fastidious aerobic actinomycetes such as gordonia, rhodococcus, and tsukamurella has remained a challenge leading to clinically significant misclassifications. this study is intended to examine the feasibility of partial 5'-end 16s rrna gene sequencing for the identification of gordonia, rhodococcus, and tsukamurella, and defined potential reference sequences for species from each of these genera. | 2011 | 21240067 |
| thoracic emphysematous lymphadenitis in a foal. | emphysema of lymph nodes is a rare finding that has been described in different anatomical locations and related to specific diseases in different animal species. herein is described a foal with rhodococcus equi infection that presented with emphysema and granulomatous inflammation of the bronchial and mediastinal lymph nodes. this is the first report of emphysematous lymphadenitis in a horse. | 2011 | 21251670 |
| effects of inactivated parapoxvirus ovis on the cumulative incidence of pneumonia and cytokine secretion in foals on a farm with endemic infections caused by rhodococcus equi. | the objectives of the present study were to determine if administration of inactivated parapoxvirus ovis (ippvo) can decrease the cumulative incidence of pneumonia and increase the number of ifn-?- and il-4-secreting cells among foals. fifty-nine foals were randomly assigned to 2 treatment groups (ippvo or placebo) prior to birth. at 24-48 h of age, foals received 2 ml of either ippvo or a placebo by intramuscular injection. injections were repeated 24h and 8 days later. the number of ifn-?- and ... | 2011 | 21255847 |
| acquired antimicrobial resistance in equine rhodococcus equi isolates. | | 2011 | 21257598 |
| [oxidative biotransformation of thioanisole by rhodococcus rhodochrous iegm 66 cells]. | comparative study of sulfoxidation activity of free and immobilized rhodococcus rhodochrous iegm 66 cells was performed. free rhodococcus cells (in the presence of 0.1 vol % n-hexadecane) displayed maximal oxidative activity towards thioanisole (0.5 g/l), a prochiral organic sulfide, added after 48-h cultivation of bacterial cells. higher sulfide concentrations inhibited sulfoxidation activity of rhodococcus. use of immobilized cells allowed the 2-day preparatory stage to be omitted and a comple ... | 2010 | 21261073 |
| [degradation of chlorinated biphenyls and products of their bioconversion by rhodococcus sp. b7a strain]. | strain rhodococcus sp. b7a isolated from artificially polluted soil destructs mono- and di-substituted ortho- and/or para-chlorinated biphenyls with utilization of chlorinated benzoic acids and shows high degradation activity as regards trichlorinated biphenyls. it is shown that p-hydroxybenzoic and protocatehoic acids are the products of p-chlorobenzoic acid catabolism. | 2010 | 21261074 |
| [intensification of surfactant synthesis in rhodococcus erythropolis ek-1 cultivated on hexadecane]. | activity of key enzymes of n-alkane metabolism was determined in cells of rhodococcus erythropolis ek-1, a surfactant producer grown on n-hexadecane. potassium cations were found to inhibit alkane hydroxylase and nadp(+)-dependent aldehyde dehydrogenase, while sodium cations were found to activate these enzymes. decreased potassium concentration (to 1 mm), increased sodium concentration (to 35 mm), and addition of 36 micromol/l fe(ii), required for alkane hydroxylase activity, resulted in increa ... | 2010 | 21261075 |
| molecular and biochemical characterization of the tetralin degradation pathway in rhodococcus sp. strain tfb. | the tetralin biodegradation pathway in rhodococcus sp. strain tfb, a gram-positive bacterium resistant to genetic manipulation, was characterized using a proteomic approach. relative protein expression in cell free extracts from tetralin- and glucose-grown cells was compared using the 2d-dige technique. identification of proteins specifically expressed in tetralin-grown cells was used to characterize a complete set of genes involved in tetralin degradation by reverse genetics. we propose a tetra ... | 2009 | 21261920 |
| rhodococcus equi human clinical isolates enter and survive within human alveolar epithelial cells. | rhodococcus equi is an emerging opportunistic human pathogen associated with immunosuppressed people, especially those infected with the human immunodeficiency virus (hiv). this pathogen resides primarily within lung macrophages of infected patients, which may explain in part its ability to escape normal pulmonary defense mechanisms. despite numerous studies as a pulmonary pathogen in foals, where a plasmid seems to play an important role in virulence, information on the pathogenesis of this pat ... | 2011 | 21262372 |
| [diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from xiaoxi national natural reserve, hunan province]. | to investigate the diversity of cultivable halophilic and halotolerant bacteria isolated from ordinary non-saline soil samples collected from xiaoxi national natural reserve (28 degrees 42' 15"-28 degrees 53' 15" n, 110 degrees 6' 50"-110 degrees 21' 35" e), hunan province, china. | 2010 | 21268889 |
| pulmonary rhodococcosis in a cat. | feline rhodococcus equi infection is rare, despite the bacteria is widespread in the environment. r equi infection is typically observed in equine species but the infection has also been reported in dogs, cats and other domestic animals. there are a few reports regarding pulmonary r equi infection in cats and the disease appears to be limited to the skin and the subcutaneous tissue. this report describes the pathological, microbiological and the virulence features associated with an acute necros ... | 2011 | 21273105 |
| homologous gene clusters of nicotine catabolism, including a new ?-amidase for a-ketoglutaramate, in species of three genera of gram-positive bacteria. | gram-positive soil bacteria arthrobacter nicotinovorans, nocardioides sp. js614 and rhodococcus opacus were shown to contain similarly organized clusters of homologous genes for nicotine catabolism. an uncharacterized gene of a predicted nitrilase within these gene clusters was cloned from a. nicotinovorans and biochemical data unexpectedly showed that the protein exhibited ?-amidase activity toward a-ketoglutaramate. structural modelling of the protein suggested the presence of the catalytic tr ... | 2011 | 21288482 |
| gamma-caprolactone stimulates growth of quorum-quenching rhodococcus populations in a large-scale hydroponic system for culturing solanum tuberosum. | bacteria degrading quorum sensing (qs) signals have been proposed as biocontrol agents able to quench qs-dependent expression of virulence symptoms caused by pectobacterium on potato plants. we report here that gamma-caprolactone (gcl) treatment stimulated growth of the native qs-degrading bacterial community in an industrial plant hydroponic system for culturing solanum tuberosum. post-gcl treatment, qs-degrading bacteria were mainly identified as rhodococcus isolates, while agrobacterium isola ... | 2011 | 21288487 |
| substrate specificity of haloalkane dehalogenases. | an enzyme's substrate specificity is one of its most important characteristics. the quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate-specificity data and analysis using multivariate statistics. we describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. the enzymes were characterized experimentally using a set of 30 ... | 2011 | 21294712 |
| isolation and characterization of rhodococcus sp. nb5 capable of degrading a high concentration of nitrobenzene. | an efficient nitrobenzene degrading bacterium strain nb5, which was able to utilize nitrobenzene as a sole source of carbon, nitrogen and energy under aerobic condition, was isolated from activated sludge in an oil refinery at hangzhou, china. based on phenotypic features, 16s rdna gene sequencing and g + c content analysis, strain nb5 was identified as rhodococcus sp. nb5. nitrobenzene degradation experiments using high performance liquid chromatograph (hplc) showed that strain nb5 could tolera ... | 2011 | 21298674 |
| crystallization and preliminary x-ray diffraction analysis of the wild-type haloalkane dehalogenase dhaa and its variant dhaa13 complexed with different ligands. | haloalkane dehalogenases make up an important class of hydrolytic enzymes which catalyse the cleavage of carbon-halogen bonds in halogenated aliphatic compounds. there is growing interest in these enzymes owing to their potential use in environmental and industrial applications. the haloalkane dehalogenase dhaa from rhodococcus rhodochrous ncimb 13064 can slowly detoxify the industrial pollutant 1,2,3-trichloropropane (tcp). structural analysis of this enzyme complexed with target ligands was co ... | 2011 | 21301099 |
| bioconversion of acrylonitrile to acrylic acid by rhodococcus ruber strain aksh-84. | a new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as rhodococcus ruber aksh-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. a simple and rapid hplc protocol was optimized for quantification of acrylic acid, acrylamide, and acrylonitrile. the optimal medium conditions for nitrilase activity were ph of 7.0, temperature of 30degreesc, agitation of 150 rpm, and ... | 2011 | 21301190 |
| bacteremia due to rhodococcus equi in an immunocompetent infant. | rhodococcus equi , previously known as corynebacterium equi, is one of the most important causes of zoonotic infection in grazing animals. increased cases of human infection with r. equi have been reported especially in immunocompromised patients. infection in immunocompetent patients is extremely rare. we report a case of r. equi bacteremia in a 26-day-old immunocompetent infant with recurrent swellings on different parts of the body. to the best of our knowledge, this is the first ever report ... | 2011 | 21304200 |
| immunohistochemical and taqman real-time pcr detection of mycobacterial infections in fish. | real-time pcr and immunohistochemistry (ihc) assays were developed to detect fish mycobacterial infections at the genus level, based on the rna polymerase ß subunit (rpob) gene and polyclonal anti-mycobacterium rabbit serum, respectively. the pcr assay positively identified a number of pathogenic mycobacteria including mycobacterium abscessus, m. avium ssp. avium, m. bohemicum, m. chelonae ssp. chelonae, m. farcinogenes, m. flavescens, m. fortuitum ssp. fortuitum, m. gastri, m. gordonae, m. immu ... | 2011 | 21306590 |
| development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways. | rhodococcus jostii rha1 accumulates chlorobenzoates (cba) during the degradation of polychlorinated biphenyls (pcbs). cba degradation is considered one of the rate-limiting steps in the complete degradation of pcbs. to reduce the accumulation of cbas, the upper pathway enzyme genes for pcb degradation of rha1 were introduced into a cba-degrading bacterium, burkholderia sp. nk8. the resulting recombinant strain exhibited no biphenyl 2,3-dioxygenase (bpha) activity encoded by bphaaabacad genes, wh ... | 2011 | 21310654 |
| comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries. | the preparation of enantiopure tertiary alcohols is of great contemporary interest due to the application of these versatile building blocks in organic synthesis and as precursors towards high value pharmaceutical compounds. herein, we describe two approaches taken towards the discovery of novel biocatalysts for the synthesis of these valuable compounds. the first approach was initiated with screening of 47 bacterial strains for hydrolytic activity towards the simple tertiary alcohol ester tert- ... | 2011 | 21318363 |
| chromosome diversity and similarity within the actinomycetales. | many chromosomes from actinomycetales, an order within the actinobacteria, have been sequenced over the last 10 years and the pace is increasing. this group of gram-positive and high g+c% bacteria is economically and medically important. however, this group of organisms also is just about the only order in the kingdom bacteria to have a relatively high proportion of linear chromosomes. chromosome topology varies within the order according to the genera. streptomyces, kitasatospora and rhodococcu ... | 2011 | 21320158 |
| isolation and characterization of rdx-degrading rhodococcus species from a contaminated aquifer. | groundwater contamination by the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) is a global problem. israel's coastal aquifer was contaminated with rdx. this aquifer is mostly aerobic and we therefore sought aerobic bacteria that might be involved in natural attenuation of the compound in the aquifer. rdx-degrading bacteria were captured by passively sampling the indigenous bacteria onto sterile sediments placed within sampling boreholes. aerobic rdx biodegradation potential was detecte ... | 2011 | 21327803 |
| a highly efficient adh-coupled nadh-recycling system for the asymmetric bioreduction of carbon-carbon double bonds using enoate reductases. | the asymmetric bioreduction of activated alkenes catalyzed by flavin-dependent enoate reductases from the oye-family represents a powerful method for the production of optically active compounds. for its preparative-scale application, efficient and economic nadh-recycling is crucial. a novel enzyme-coupled nadh-recycling system is proposed based on the concurrent oxidation of a sacrificial sec-alcohol catalyzed by an alcohol dehydrogenase (adh-a). due to the highly favorable position of the equi ... | 2011 | 21328323 |
| virulence quenching with a prenylated isoflavanone renders the malagasy legume dalbergia pervillei resistant to rhodococcus fascians. | the phytopathogenic actinomycete rhodococcus fascians induces leafy galls on a wide range of hosts, causing major economical losses in the ornamentals industry. although differences in the responsivity occur within species, no plant tested so far could be considered resistant to r. fascians strain d188 infection. here, we observed that members of the genus dalbergia, which belong to the fabaceae, did not develop leafy galls when challenged with r. fascians and we set out to unravel the mechanism ... | 2011 | 21332623 |
| glucose-mediated transcriptional repression of pcb/biphenyl catabolic genes in rhodococcus jostii rha1. | rhodococcus jostii rha1, a gram-positive polychlorinated biphenyl (pcb) degrader, exhibited biphasic growth in a medium containing biphenyl (bph) and glucose (glc), with consumption of glc and low 2,3-dihydroxybiphenyl 1,2-dioxygenase activity in the first growth stage. these results suggested the repression of bph metabolism in the presence of glc, in which rha1 preferentially utilized glc in the first growth stage and bph in the second stage. a reporter assay using a transcriptional fusion of ... | 2011 | 21335979 |
| [significance of evaluation of the antibiotic-resistant properties of bacterial producing strains in the hygienic standardization system]. | the study was undertaken to evaluate the antibiotic sensitivity of microorganisms that were used in biotechnological production and belonged to different taxons: gram-positive (bacillus subtilis, bacillus licheniformis, and bacillus thuringiensis) and gram-negative (alcaligenes denitrificans and pseudomonas caryophylii) bacteria and noncardioform actinomycetes (rhodococcus erythropolis and rhodococcus corallinus). the sensitivity of the strains to a range of antibiotics was determined by the aga ... | 2010 | 21344698 |
| the pe-ppe domain in mycobacterium reveals a serine a/ß hydrolase fold and function: an in-silico analysis. | the pe and ppe proteins first reported in the genome sequence of mycobacterium tuberculosis strain h37rv are now identified in all mycobacterial species. the pe-ppe domain (pfam id: pf08237) is a 225 amino acid residue conserved region located towards the c-terminus of some pe and ppe proteins and hypothetical proteins. our in-silico sequence analysis revealed that this domain is present in all mycobacteria, some rhodococcus and nocardia farcinica genomes. this domain comprises a pentapeptide se ... | 2011 | 21347309 |
| adventures in rhodococcus - from steroids to explosives. | rhodococcus is a genus of mycolic-acid-containing actinomycetes that utilize a remarkable variety of organic compounds as growth substrates. this degradation helps maintain the global carbon cycle and has increasing applications ranging from the biodegradation of pollutants to the biocatalytic production of drugs and hormones. we have been using rhodococcus jostii rha1 as a model organism to understand the catabolic versatility of rhodococcus and related bacteria. our approach is exemplified by ... | 2011 | 21358756 |
| enzymes hydrolyzing structural components and ferrous ion cause rusty-root symptom on ginseng (panax ginseng). | microbial induction of rusty-root was proved in this study. the enzymes hydrolyzing plant structural materials, including pectinase, pectolyase, ligninase, and cellulase, caused the rusty-root in ginseng. pectinase and pectolyase produced the highest rusty-color formation. ferrous ion (fe+++) caused the synergistic effect on rusty-root formation in ginseng when it was used with pectinase. the effect of ferric ion (fe++) on rusty-root formation was slow, compared with fe+++, probably due to gradu ... | 2011 | 21364303 |
| [purification and characterization of a ph-stable and thermostable biphenyl hydrolase from rhodococcus sp. r04]. | biphenyl hydrolase (bphd) is an enzyme of the biphenyl biodegradation pathway in rhodococcus sp. r04. expression, purification and properties of bphd, together with chemical modification were investigated. | 2010 | 21365917 |
| facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium. | we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ... | 2011 | 21374057 |
| probing the enantioselectivity of a diverse group of purified cobalt-centred nitrile hydratases. | in this study a diverse range of purified cobalt containing nitrile hydratases (nhases, ec 4.2.1.84) from rhodopseudomonas palustris haa2 (haa2), rhodopseudomonas palustris cga009 (009), sinorhizobium meliloti 1021 (1021), and nitriliruptor alkaliphilus (iso2), were screened for the first time for their enantioselectivity towards a broad range of chiral nitriles. enantiomeric ratios of >100 were found for the nhases from haa2 and cga009 on 2-phenylpropionitrile. in contrast, the fe-containing nh ... | 2011 | 21380438 |
| biosynthesis of the siderophore rhodochelin requires the coordinated expression of three independent gene clusters in rhodococcus jostii rha1. | in this work we report the isolation, structural characterization, and the genetic analysis of the biosynthetic origin of rhodochelin, a unique mixed-type catecholate-hydroxamate siderophore isolated from rhodococcus jostii rha1. rhodochelin structural elucidation was accomplished via ms(n)- and nmr-analysis and revealed the tetrapeptide to contain an unusual ester bond between an l-+¦-n-formyl-+¦-n-hydroxyornithine moiety and the side chain of a threonine residue. gene deletions within three pu ... | 2011 | 21381663 |
| sonographic characteristics of intraabdominal abscessation and lymphadenopathy attributable to rhodococcus equi infections in foals. | the sonographic characteristics of intraabdominal abscesses in 11 foals and intraabdominal lymphadenitis in five foals with rhodococcus equi infections are presented. intraabdominal abscesses were usually present in the ventral abdomen adjacent to the ventral body wall, well-marginated, and contained a mixed or complex echo pattern. lymphadenitis appeared as singular or multiple clusters of lymph nodes of mixed echogenicity adjacent to any portion of the gastrointestinal tract or body wall. sono ... | 2011 | 21382118 |
| effect of propionibacterium acnes-containing immunostimulant on interferon-gamma (ifn+¦) production in the neonatal foal. | production of the th1 cytokine interferon gamma (ifn+¦) is associated with resistance to intracellular pathogens, including rhodococcus equi. while neonatal foals are initially deficient in ifn+¦ production, expression of this cytokine increases throughout their first year of life. this is presumably the result of stimulation by environmental antigens including pathogen associated molecular patterns (pamps) signaling through toll-like receptors (tlr). this increased expression of ifn+¦ is likewi ... | 2011 | 21382640 |
| nitric oxide-mediated intracellular growth restriction of pathogenic rhodococcus equi can be prevented by iron. | rhodococcus equi is an intracellular pathogen which causes pneumonia in young horses and in immunocompromised humans. r. equi arrests phagosome maturation in macrophages at a prephagolysosome stage and grows inside a privileged compartment. here, we show that, in murine macrophages activated with gamma interferon and lipopolysaccharide, r. equi does not multiply but stays viable for at least 24 h. whereas infection control of other intracellular pathogens by activated macrophages is executed by ... | 2011 | 21383050 |
| crystallization and crystallographic analysis of the rhodococcus rhodochrous ncimb 13064 dhaa mutant dhaa31 and its complex with 1,2,3-trichloropropane. | haloalkane dehalogenases hydrolyze carbon-halogen bonds in a wide range of halogenated aliphatic compounds. the potential use of haloalkane dehalogenases in bioremediation applications has stimulated intensive investigation of these enzymes and their engineering. the mutant dhaa31 was constructed to degrade the anthropogenic compound 1,2,3-trichloropropane (tcp) using a new-ástrategy. this strategy enhances activity towards tcp by decreasing the accessibility of the active site to water molecule ... | 2011 | 21393851 |
| horizontal gene transfer (hgt) as a mechanism of disseminating rdx-degrading activity among actinomycete bacteria. | hexahydro-1,3,5-trinitro-1,3,5,-triazine (rdx) is a cyclic nitramine explosive that is a major component in many high-explosive formulations and has been found as a contaminant of soil and groundwater. the rdx-degrading gene locus xplab, located on pgkt2 in gordonia sp. ktr9, is highly conserved among isolates from disparate geographical locations suggesting a horizontal gene transfer (hgt) event. it was our goal to determine whether gordonia sp. ktr9 is capable of transferring pgkt2 and the ass ... | 2011 | 21395947 |
| identification and characterization of another 4-nitrophenol degradation gene cluster, nps, in rhodococcus sp. strain pn1. | 4-nitrophenol (4-np) is a toxic compound formed in soil by the hydrolysis of organophosphorous pesticides, such as parathion. we previously reported the presence of the 4-np degradation gene cluster (nphra1a2) in rhodococcus sp. strain pn1, which encodes a two-component 4-np hydroxylase system that oxidizes 4-np into 4-nitrocatechol. in the current study, another gene cluster (npsc and npsra2a1b) encoding a similar 4-np hydroxylase system was cloned from strain pn1. the enzymes from this 4-np hy ... | 2011 | 21396889 |
| identification of novel potential antibiotics for tuberculosis by in silico structure-based drug screening. | the enoyl-acyl carrier protein reductase of mycobacterium tuberculosis (mtb) is a key enzyme of the type ii fatty acid synthesis system. it is involved in the production of mycolic acid and is a known target for isoniazid, an effective antibiotic for tuberculosis treatment. the increasing prevalence of tuberculosis in many areas of the world, which is associated with the rise of drug-resistant mtb strains, presents a major global health threat. in this study, we attempted to identify novel antib ... | 2011 | 21397998 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered ralstonia eutropha strains. | the polyhydroxyalkanoate (pha) copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(hb-co-hhx)] has been shown to have potential to serve as a commercial bioplastic. synthesis of p(hb-co-hhx) from plant oil has been demonstrated with recombinant ralstonia eutropha strains expressing heterologous pha synthases capable of incorporating hb and hhx into the polymer. with these strains, however, short-chain-length fatty acids had to be included in the medium to generate pha with high hhx conten ... | 2011 | 21398488 |
| preliminary report on a catalyst derived from induced cells of rhodococcus rhodochrous strain dap 96253 that delays the ripening of selected climacteric fruit: bananas, avocados, and peaches. | despite the use of refrigeration, improved packaging, adsorbents, and ethylene receptor blockers, on average, nearly 40% of all fruits and vegetables harvested in the us are not consumed. many plant products, especially fruit, continue to ripen after harvesting, and as they do so, become increasingly susceptible to mechanical injury, resulting in increased rot. other plant products during transportation and storage are susceptible to chill injury (ci). there is a real need for products that can ... | 2011 | 21409422 |
| application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents. | a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ... | 2011 | 21419572 |
| novel bacterial strains for the removal of microcystins from drinking water. | microcystins (mc) and nodularin (nod) are common contaminants of drinking water around the world and due to their significant health impact it is important to explore suitable approaches for their removal. unfortunately, these toxins are not always removed by conventional water treatments. one of the most exciting areas that hold promise for a successful and cost effective solution is bioremediation of microcystins. recent work resulted in successful isolation and characterisation of 10 novel ba ... | 2011 | 21436548 |
| plasma pharmacokinetics, pulmonary distribution, and in vitro activity of gamithromycin in foals. | berghaus, l. j., giguère, s., sturgill, t. l., bade, d., malinski, t. j., huang, r. plasma pharmacokinetics, pulmonary distribution, and in vitro activity of gamithromycin in foals. j. vet. pharmacol. therap. doi: 10.1111/j.1365-2885.2011.01292.x. the objectives of this study were to determine the plasma and pulmonary disposition of gamithromycin in foals and to investigate the in vitro activity of the drug against streptococcus equi subsp. zooepidemicus (s. zooepidemicus) and rhodococcus equi. ... | 2011 | 21443748 |
| pivalic acid acts as a starter unit in a fatty acid and antibiotic biosynthetic pathway in alicyclobacillus, rhodococcus and streptomyces. | a biosynthetic pathway using pivalic acid as a starter unit was found in three bacterial species, alicyclobacillus acidoterrestris, rhodococcus erythropolis and streptomyces avermitilis. when deuterium-labelled pivalic acid was added to a. acidoterrestris and r. erythropolis nutrient media it was incorporated into fatty acids to give rise to tert-butyl fatty acids (t-fas). in addition, in r. erythropolis, pivalic acid was transformed into two starter units, i.e. isobutyric and 2-methylbutyric ac ... | 2011 | 21450004 |
| bacterial and plant signal integration via d3-type cyclins enhances symptom development in the arabidopsis-rhodococcus fascians interaction. | the phytopathogenic actinomycete rhodococcus fascians drives its host to form a nutrient-rich niche by secreting a mixture of cytokinins that triggers plant cell division and shoot formation. the discrepancy between the relatively low amount of secreted cytokinins and the severe impact of r. fascians infection on plant development has puzzled researchers for a long time. polyamine and transcript profiling of wild-type and cytokinin receptor mutant plants revealed that the bacterial cytokinins di ... | 2011 | 21459976 |
| maternal immunization with actinomycetales immunomodulators reduces parasitemias in offspring challenged with trypanosoma cruzi. | this article describes the first use of heat-killed, borate-buffered preparations of aerobic actinomycetales to immunize pregnant animals in order to determine the effect on their pregnancy and fertility and the survival coefficients of their offspring. pregnant rats received three injections of gordonia bronchialis, rhodococcus coprophylus or physiological saline and a proportion of their offspring were challenged with live trypanosoma cruzi at the time of weaning. levels of parasitemia and, in ... | 2011 | 21463197 |
| identification and characterization of uptake systems for glucose and fructose in rhodococcus jostii rha1. | rhodococcusjostii rha1 takes up and utilizes glucose and fructose as its sole carbon source. to address glucose and fructose transport systems in rha1, an in silico analysis was performed. blast searches revealed that the ro02365 and ro06844 genes share 51 and 47% amino acid identity, respectively, with the major facilitator superfamily (mfs) glucose transporter, glcp, of streptomycescoelicolor a3(2) and the ro01549-01552 and ro06781-06785 loci share 43-51% amino acid identity with proteins of t ... | 2011 | 21464575 |
| multidrug resistance in hydrocarbon-tolerant gram-positive and gram-negative bacteria. | new gram-positive and gram-negative bacteria were isolated from poeni oily sludge, using enrichment procedures. the six gram-positive strains belong to bacillus, lysinibacillus and rhodococcus genera. the eight gram-negative strains belong to shewanella, aeromonas, pseudomonas and klebsiella genera. isolated bacterial strains were tolerant to saturated (i.e., n-hexane, n-heptane, n-decane, n-pentadecane, n-hexadecane, cyclohexane), monoaromatic (i.e., benzene, toluene, styrene, xylene isomers, e ... | 2011 | 21478643 |
| early development of cytotoxic t lymphocytes in neonatal foals following oral inoculation with rhodococcus equi. | rhodococcus equi is an important respiratory pathogen of young foals for which a vaccine has long been sought. two major impediments to effective vaccination are the functionally immature type i immune responses of neonatal foals and early exposure to the bacterium via the environment. despite these obstacles, it appears that under specific circumstances foals can develop a protective immune response. in this study we investigated the protective mechanisms behind oral inoculation of foals with v ... | 2011 | 21481947 |
| structure-based redesign of cofactor binding in putrescine oxidase. | putrescine oxidase (puo) from rhodococcus erythropolis is a soluble homodimeric flavoprotein, which oxidizes small aliphatic diamines. in this study, we report the crystal structures and cofactor binding properties of wild-type and mutant enzymes. from a structural viewpoint, puo closely resembles the sequence-related human monoamine oxidases a and b. this similarity is striking in the flavin-binding site even if puo does not covalently bind the cofactor as do the monoamine oxidases. a remarkabl ... | 2011 | 21486042 |
| a successful bacterial coup d'état: how rhodococcus fascians redirects plant development. | rhodococcus fascians is a gram-positive phytopathogen that induces differentiated galls, known as leafy galls, on a wide variety of plants employing virulence genes located on a linear plasmid. the pathogenic strategy consists in the production of a mixture of six synergistically acting cytokinins that overwhelm the plant's homeostatic mechanisms, ensuring the activation of a signaling cascade that targets the plant cell cycle and directs the newly formed cells to differentiate into shoot merist ... | 2010 | 21495844 |
| genetic diversity of root nodule bacteria nodulating lotus corniculatus and anthyllis vulneraria in sweden. | very little is known about the genetic diversity and phylogeny of rhizobia nodulating lotus species in northern temperate regions. we have therefore studied the genetic diversity among a total of 61 root nodule bacteria isolated from lotus corniculatus and anthyllis vulneraria from different geographic sites and habitats in sweden by restriction fragment length polymorphism (rflp) of the internal transcribed spacer between their 16s rrna and 23s rrna (igs) region. a high diversity consisting of ... | 2011 | 21497473 |
| culturable bacteria in hydroponic cultures of moss racomitrium japonicum and their potential as biofertilizers for moss production. | the use of racomitrium japonicum, a drought resistant bryophyte used for roof-greening, is gradually increasing. however, its utilization is hampered by slow growth rate. here we isolated culturable bacteria from hydroponic cultivation samples to identify isolates that could promote moss growth. most of the isolates belonged to pseudomonas, rhodococcus, and duganella species. the isolates were biochemically characterized according to their type of interaction with plants, i.e., production of aux ... | 2011 | 21498111 |
| genome characterization of the polyvalent lytic bacteriophage gte2 with the potential for biocontrol of gordonia, rhodococcus and nocardia stabilized foams in activated sludge plants. | hydrophobic actinobacteria are commonly associated with the stabilization of foams in activated sludge systems. one possible attractive approach to control these foam stabilizing organisms is to use specific bacteriophages. we describe the genome characterization of a novel polyvalent dna phage, gte2, isolated from activated sludge. this phage is lytic for gordonia terrae, rhodococcus globerulus, rhodococcus erythropolis, rhodococcus erythropolis, nocardia otitididiscaviarum, and nocardia brasil ... | 2011 | 21498753 |
| biodegradation of sulfamethoxazole by individual and mixed bacteria. | antibiotic compounds, like sulfamethoxazole (smx), have become a concern in the aquatic environment due to the potential development of antibacterial resistances. due to excretion and disposal, smx has been frequently detected in wastewaters and surface waters. smx removal in conventional wastewater treatment plants (wwtps) ranges from 0% to 90%, and there are opposing results regarding its biodegradability at lab scale. the objective of this research was to determine the ability of pure culture ... | 2011 | 21499763 |
| naphthalene-degrading bacteria of the genus rhodococcus from the verkhnekamsk salt mining region of russia. | eight moderately halotolerant naphthalene-degrading strains of the genus rhodococcus isolated from soil samples and slime pit bottom sediment of the verkhnekamsk salt mining region of russia were characterized by pcr amplification of repetitive bacterial dna elements (rep-pcr) and identified by 16s ribosomal rna gene sequence analysis. the diversity of their dioxygenase (nar-like) genes was investigated as these genes are known to be involved in naphthalene-degradation. the analysis of the nar-l ... | 2011 | 21503712 |
| crystallization and preliminary x-ray diffraction analysis of recombinant chlorocatechol 1,2-dioxygenase from pseudomonas putida. | chlorocatechol 1,2-dioxygenase from the gram-negative bacterium pseudomonas putida (pp 1,2-ccd) is considered to be an important biotechnological tool owing to its ability to process a broad spectrum of organic pollutants. in the current work, the crystallization, crystallographic characterization and phasing of the recombinant pp 1,2-ccd enzyme are described. reddish-brown crystals were obtained in the presence of polyethylene glycol and magnesium acetate by utilizing the vapour-diffusion techn ... | 2011 | 21505253 |
| hydrocarbon-utilizing microorganisms naturally associated with sawdust. | sawdust, one of the materials used as sorbent for removing spilled oil from polluted environments was naturally colonized by hydrocarbon-utilizing fungi, 1×10(5)-2×10(5) colony forming units (cfu) g(-1), depending on the hydrocarbon substrate. this sorbent was initially free of hydrocarbon-utilizing bacteria. incubating wet sawdust at 30°c resulted in gradually increasing the fungal counts to reach after 6months between 5×10(6) and 7×10(6)cfug(-1), and the appearance of hydrocarbon-utilizing bac ... | 2011 | 21507457 |