| survey of infectious agents in the endangered darwin's fox (lycalopex fulvipes): high prevalence and diversity of hemotrophic mycoplasmas. | very little is known about the diseases affecting the darwin's fox (lycalopex fulvipes), which is considered to be one of the most endangered carnivores worldwide. blood samples of 30 foxes captured on chiloé island (chile) were tested with a battery of pcr assays targeting the following pathogens: ehrlichia/anaplasma sp., rickettsia sp., bartonella sp., coxiella burnetti, borrelia sp., mycoplasma sp., babesia sp., hepatozoon canis, hepatozoon felis, leishmania donovani complex, and filariae. an ... | 2013 | 24176254 |
| development and evaluation of serodiagnostic assays with recombinant bgsa1 of babesia gibsoni. | indirect elisa, dot-elisa and double antibody sandwich elisa (das-elisa) using truncated recombinant bgsa1 (rbgsa1) were developed for detecting babesia gibsoni infection in naturally infected dogs. truncated bgsa1 gene of 858 bp, encoding 32 kda protein was cloned in pet-32a(+) expression vector, expressed in escherichia coli and the recombinant protein was purified under native conditions. to evaluate the ability of the truncated rbgsa1as serodiagnostic reagent for b. gibsoni infection, a pane ... | 2014 | 25241636 |
| rhipicephalus turanicus, a new vector of hepatozoon canis. | the distribution of hepatozoon canis mainly encompasses areas where its main tick vector, rhipicephalus sanguineus sensu lato, is present. however, the detection of this pathogen in dogs, foxes and golden jackals well outside the areas inhabited by this tick species reinforced the hypothesis that additional ixodids are involved in the life cycle and transmission of this protozoon. the present study provides, for the first time, data supporting the sporogonic development of h. canis in specimens ... | 2016 | 27998321 |
| detection and molecular identification of hepatozoon canis and babesia vogeli from domestic dogs in palestine. | dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. this study aimed to observe tick borne pathogens in dogs from palestine including hepatozoon canis and babesia species. the prevalence of both h. canis and babesia species infections in apparently healthy dogs, from ten districts of the west bank was surveyed. dna was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). a primer set that amplifi ... | 2017 | 27938422 |
| hepatozoon canis and leishmania spp. coinfection in dogs diagnosed with visceral leishmaniasis. | this study describes the occurrence of dogs naturally co-infected with hepatozoon canis and two leishmania species: l. infantum or l. braziliensis. four dogs serologically diagnosed with visceral leishmaniasis were euthanized. liver and spleen samples were collected for histopathological analysis and dna isolation. h. canis meronts were observed in tissues from all four dogs. h. canis infection was confirmed by pcr followed by sequencing of a fragment of 18s rrna gene. leishmania detection and t ... | 2016 | 27925060 |
| molecular detection and prevalence of hepatozoon canis in dogs from punjab (pakistan) and hematological profile of infected dogs. | the intraleukocytic parasite, hepatozoon canis, causes the sometimes fatal tick borne disease canine hepatozoonosis. in this study, dogs from islamabad, lahore, and multan districts of the punjab region of pakistan were surveyed to investigate the presence and prevalence of h. canis infection and to determine the effects of the parasite on hematological parameters. blood samples were collected from 151 domestic dogs (149 pet, 2 stray) of both sexes and varying ages. data on sex, age, tick infest ... | 2017 | 27841956 |
| investigation of tick vectors of hepatozoon canis in brazil. | hepatozoon canis is a common apicomplexan parasite of dogs. in brazil, in addition to rhipicephalus sanguineus, amblyomma ovale, amblyomma cajennense, and rhipicephalus (boophilus) microplus have been suggested to act as vectors. the present study aimed to evaluate, under controlled conditions, the acquisition of h. canis by a. ovale, r. sanguineus, and a. cajennense after feeding on naturally infected dogs. cytological and histophatological examinations were performed to recover oocysts and oth ... | 2013 | 24209494 |
| transstadial transmission of hepatozoon canis from larvae to nymphs of rhipicephalus sanguineus. | hepatozoon canis is an apicomplexan parasite of dogs, which is known to become infected by ingesting rhipicephalus sanguineus adult ticks. to investigate the possibility of h. canis transovarial and transstadial transmission from larvae to nymphs, engorged adult female ticks were collected from a private animal shelter in southern italy, where h. canis infection is highly prevalent. female ticks (n=35) and egg batches were tested by pcr for h. canis. all eggs examined were pcr-negative whereas 8 ... | 2013 | 23537949 |
| molecular investigations of hepatozoon species in dogs and developmental stages of rhipicephalus sanguineus. | the occurrence and distribution of hepatozoon species in stray dogs, and the developmental stages of rhipicephalus sanguineus detached from the same dogs in diyarbakır province, turkey is reported. a total of 328 ticks, including 133 adults (55 males and 75 females consist of 63 partially engorged and 15 fully engorged) and 195 nymphs (91 partially engorged and 104 fully engorged) were detached from the dogs. fully engorged nymphs and females were incubated at 27 °c and relative humidity of 85 % ... | 2013 | 23535887 |
| high prevalence of hepatozoon-infection among shepherd dogs in a region considered to be free of rhipicephalus sanguineus. | blood samples and ticks were collected from 100 shepherd dogs, 12 hunting dogs and 14 stray dogs in southern hungary, in order to screen them for the presence of hepatozoon spp. by pcr. out of 126 blood samples, 33 were positive (26%). significantly more shepherd dogs (31%) were infected, than hunting (8%) and stray dogs (7%). three genotypes of hepatozoon canis were identified by sequencing, differing from each other in up to six nucleotides in the amplified portion of their 18s rrna gene. in d ... | 2013 | 23499483 |
| a golden jackal (canis aureus) from austria bearing hepatozoon canis--import due to immigration into a non-endemic area? | the protozoan hepatozoon canis, which is transmitted via ingestion of infected ticks by canine hosts, is not endemic to mid-latitude regions in europe. its distribution is supposed to be linked to the occurrence of its primary tick vector rhipicephalus sanguineus. a young male golden jackal (canis aureus) found as road kill close to vienna, austria, was infected by this pathogen. cloning and sequencing of the pcr product revealed 6 different haplotypes of h. canis. based on the sequences, no cle ... | 2013 | 23306030 |
| apparent tick paralysis by rhipicephalus sanguineus (acari: ixodidae) in dogs. | certain tick species including ixodes holocyclus can inoculate neurotoxins that induce a rapid, ascending flaccid paralysis in animals. rhipicephalus sanguineus, the most widespread tick of dogs, is recognized as a vector of several pathogens causing diseases in dogs and humans. a single report suggests its role as cause of paralysis in dogs. this study presents the clinical history of 14 young dogs heavily infested by r. sanguineus (intensity of infestation, 63-328) in an endemic area of southe ... | 2012 | 22546547 |
| a new pcr assay for the detection and differentiation of babesia canis and babesia vogeli. | babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. given the reports of an expanding and, in some cases, sympatric geographical distribution of babesia canis and babesia vogeli in dogs and associated vectors, in europe, the development of time-effici ... | 2017 | 28739301 |
| a pcr survey of vector-borne pathogens in different dog populations from turkey. | in the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in konya district, turkey, and tested by specific pcr for the presence of vector-borne pathogens. several pathogens were identified, most of which can cause substantial morbidity in dogs. pcr results revealed that 54 (28.1%) dogs were infected with one or more pathogens. positive results were obtained for babesia spp. in 4 dogs (2.1%), hepatozoon spp. in 8 dogs (4.2%) and mycoplasma spp ... | 2017 | 28682765 |
| a survey of canine haemoprotozoan parasites from turkey, including molecular evidence of an unnamed babesia. | canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. a reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as hepatozoon canis. ten samples hybridized to the theileria/babesia and babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrec ... | 2017 | 28673460 |
| molecular detection of vector-borne pathogens in dogs and cats from qatar. | vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries. | 2017 | 28633666 |
| molecular detection of tick-borne pathogens in wild red foxes (vulpes vulpes) from central italy. | spleen samples from 153 red foxes, shot during regular hunting season in the province of pisa (central italy), were examined to detect dna of anaplasma phagocytophilum, ehrlichia canis, coxiella burnetii, francisella tularensis, hepatozoon canis and babesia sp./theileria sp. dna of vector-borne pathogens was detected in 120 (78.43%; 95% ci: 71.06-84.66%) foxes. specifically, 75 (49%; 95% ci: 40.86-57.22%) animals scored pcr-positive per h. canis, 68 (44.44%; 95% ci: 36.42-52.69%) for e. canis, 3 ... | 2017 | 28502642 |
| detection and molecular characterization of babesia, theileria, and hepatozoon species in hard ticks collected from kagoshima, the southern region in japan. | to reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (pcr) system to detect the most common agents of tick-borne parasitic diseases, namely babesia, theileria, and hepatozoon parasites. we collected host-seeking or animal-feeding ticks in kagoshima prefecture, the southernmost region of kyusyu island in southwestern japan. twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for ... | 2017 | 28501503 |
| acute hepatozoonosis in dogs: a case report. | two male dogs aged between 2 and 3 months belonging to german shepherd and non descript breed were brought to outpatient unit of department of veterinary medicine, veterinary college, bidar with history of anorexia, weakness and going down in body condition since 15 days. clinical examination revealed fever, enlargement of popliteal and submandibular lymph nodes, pale mucous membranes and cachectic body condition in both the cases. haemato-biochemical examination revealed anemia, leukocytosis, t ... | 2017 | 28848272 |
| didelphis albiventris naturally infected with hepatozoon canis in southeastern brazil. | hepatozoon species are vector-borne pathogens that infect domestic and wild animals. marsupials of the species didelphis albiventris are adapted to urban and peri-urban areas and act as reservoir hosts for several parasites. the present study evaluated the occurrence of infection by hepatozoon species in synantropic d. albiventris from botucatu, são paulo, brazil. blood samples and ectoparasites from 19 d. albiventris were collected from urban and peri-urban areas. hepatozoon spp. detection was ... | 2017 | 28728938 |
| babesia, theileria, and hepatozoon species in ticks infesting animal hosts in romania. | babesia spp., theileria spp., and hepatozoon spp. are tick-transmitted apicomplexan parasites that cause several important diseases in animals. to increase current knowledge about the diversity of tick-transmitted pathogens in romania, we investigated the occurrence of babesia spp., theileria spp., and hepatozoon spp. in a wide range of tick species infesting animal hosts. we collected 852 ticks from 10 different animal species from 20 counties in romania. the assessment was based on detection o ... | 2017 | 28676905 |
| three different hepatozoon species in domestic cats from southern italy. | three species of hepatozoon, namely, hepatozoon felis, hepatozoon canis and hepatozoon silvestris may affect domestic and/or wild felids. although hepatozoonosis has been documented in a wide range of mammal species, data on cats are limited. to investigate the occurrence of these pathogens in cats, blood samples were collected from animals living in three provinces of southern italy (bari, lecce, and matera), and molecularly analysed by pcr amplification and sequencing of segments of the 18s rr ... | 2017 | 28576619 |
| transstadial transmission of hepatozoon canis by rhipicephalus sanguineus (acari: ixodidae) in field conditions. | this study investigated possible transovarial and transstadial transmission of hepatozoon canis by rhipicephalus sanguineus (latreille) ticks collected from naturally infected dogs in a municipal dog shelter and the grounds of the shelter. four hundred sixty-five engorged nymphs were collected from 16 stray dogs that were found to be infected with h. canis by blood smear and pcr analyses and maintained in an incubator at 28 °c for moulting. four hundred eighteen nymphs moulted to adults 14-16 d ... | 2017 | 28399211 |