| paragonimiasis and the genus paragonimus. | the review concentrates on literature that has appeared since the 1960s. since then, numerous species of paragonimus have been described, mainly from asia but also from africa and the americas. some of these cause disease in humans. recent information on life cycles and routes of transmission is summarized. all described species and their hosts are listed, with synonyms where known. for well-known species such as paragonimus westermani, subspecific taxa and strains are reviewed and genetic studi ... | 1999 | 10050273 |
| infection status of paragonimus westermani metacercariae in crayfish (cambaroides similis) collected from bogildo (islet), wando-gun, chollanam-do, korea. | during the period from october 1996 to november 1998, the infection status of paragonimus westermani metacercariae in freshwater crayfish (cambaroides similis) collected from bogildo (islet). wando-gun, chollanam-do, which is known for an endemic area of p. westermani in korea, were examined. the average infection rate of paragonimus metacercariae in crayfish was 88.6%, and mean number of metacercariae per infected crayfish was 30.2. this metacercarial density was the highest in the group of wei ... | 1999 | 10188385 |
| [a clinical research for eosinophilic meningoencephalitis caused by angiostrongyliasis]. | to improve the awareness of angiostrongyliasis. | 1999 | 11798667 |
| occurrence of a diploid type and a new first intermediate host of a human lung fluke, paragonimus westermani, in korea. | the biology, chromosome number, and karyotype of a lung fluke, paragonimus westermani (kerbert, 1878) collected in haenam, haenam-gun chollanam-do, korea were analyzed. we compared the size of metacercariae from haenam with those taken from a crayfish collected at youngam, youngam-gun, chollanam-do, korea. the mean length of p. westermani metacercariae from haenam was 300.3 microm and that from youngam was 362.0 microm. adult worms were recovered from the lungs of experimentally infected dogs. t ... | 2001 | 11888247 |
| intra- and interindividual variation in its1 of paragonimus westermani (trematoda: digenea) and related species: implications for phylogenetic studies. | we investigated the utility of the ribosomal first internal transcribed spacer (its1) for phylogenetic studies on trematodes of the genus paragonimus. numerous clones containing its1 pcr products were sequenced for p. miyazakii, p. macrorchis, and members of the p. ohirai and p. westermani species complexes. some additional data were obtained by direct sequencing of pcr products. the its1 is composed of three distinct regions: the short 5' end, followed by a tract of approximately 120 nucleotide ... | 1999 | 10222162 |
| paragonimiasis in yongjia county, zhejiang province, china: clinical, parasitological and karyotypic studies on paragonimus westermani. | paragonimiasis in yongjia county, zhejiang province, china, has been of such public health concern that a specialized institute for paragonimiasis control has been established there. the study reported here involved both parasitological research on paragonimus westermani in the endemic areas and a clinical analysis of 94 cases of paragonimiasis. eggs were found in sputum, feces and brain tissue in 45 cases. twenty patients, 19 of whom where children under 10 years of age, showed hepatic injury. ... | 2001 | 12041551 |
| severe pleuropulmonary paragonimiasis 8 years after emigration from a region of endemicity. | a laotian man who had resided only in the north-central united states for 8 years sought care for an acute, progressive syndrome of severe dyspnea, chest pain, bilateral pneumothoraces, lung and liver nodules, and marked peripheral blood eosinophilia. he habitually ate raw crabmeat imported pickled or frozen from southeast asia; he denied eating local crustaceans. ova consistent with the lung fluke paragonimus westermani were identified in a bronchoalveolar lavage specimen, and the eosinophilia ... | 2002 | 12060881 |
| genetic diversity in parthenogenetic triploid paragonimus westermani. | paragonimus westermani is a medically important foodborne trematode occurring throughout southeast asia. we have used molecular techniques to test the hypothesis that the parthenogenetic triploid form of p. westermani has arisen only once. sources of data for comparison were: (a) restriction fragment length polymorphisms (rflps) of ribosomal internal transcribed spacers (its); and (b) 'fingerprint' patterns observed when genomic digests were probed with simple sequence repeats (att)10 and (atgt) ... | 1999 | 10579435 |
| pathologic quiz case. pulmonary infestation by paragonimus westermani. | | 2000 | 11100074 |
| [discussion on two ecological aspects of paragonimus westermani cercaria]. | | 1998 | 12078297 |
| molecular phylogeographic studies on paragonimus westermani in asia. | the lung fluke, paragonimus westermani (kerbert, 1878), is widely distributed in asia, and exhibits much variation in its biological properties. previous phylogenetic studies using dna sequences have demonstrated that samples from north-east asia form a tight group distinct from samples from south asia (philippines, thailand, malaysia). among countries from the latter region, considerable molecular diversity was observed. this was investigated further using additional dna sequences (partial mito ... | 2000 | 11138020 |
| polymerase chain reaction (pcr)-based molecular discrimination between paragonimus westermani and p. miyazakii at the metacercarial stage. | the metacercariae of the lung flukes, paragonimus westermani and p. miyazakii, are of known medical importance as the pathogens causing human paragonimiasis. they are both found in the same freshwater crab species in japan and are morphologically quite similar. the aim of the present study was to establish molecular methods for accurate discrimination between individual metacercariae of the two species. in the first step, we amplified and sequenced the second internal transcribed spacer (its2) r ... | 2002 | 12144775 |
| excretory-secretory product of newly excysted metacercariae of paragonimus westermani directly induces eosinophil apoptosis. | eosinophils are important effector cells in host defense against parasites. excretory-secretory product (esp) produced by helminthic worms plays important roles in the uptake of nutrients, migration in the host tissue, and in immune modulation. however, little is known about the ability of the esp to directly trigger eosinophil apoptosis. this study investigated whether the esp of newly excysted metacercariae of paragonimus westermani could induce apoptosis in human eosinophils. apoptosis was as ... | 2000 | 10743354 |
| elevated il-5 levels in pleural fluid of patients with paragonimiasis westermani. | to investigate the pathogenic mechanisms of eosinophilic pleural effusion in patients with paragonimiasis, we measured the levels of il-5, granulocyte-macrophage colony-stimulating factor (gm-csf) and interferon-gamma (ifn-gamma) in pleural effusions. samples were obtained from 11 patients with paragonimus westermani infection. in addition, samples from 12 patients with pleural transudates, 16 with tuberculous pleurisy, seven with empyema and 20 with lung cancer were also examined. eosinophilia ... | 2001 | 11168004 |
| a clinical study of eosinophilic meningoencephalitis caused by angiostrongyliasis. | to improve the clinician's awareness of angiostrongyliasis. | 2002 | 12411101 |
| cloning and expression of a cysteine proteinase gene from paragonimus westermani adult worms. | a gene encoding a cysteine proteinase from paragonimus westermani has been cloned and expressed in escherichia coli. the cysteine proteinase cdna fragment was amplified by reverse transcription-polymerase chain reaction (rt-pcr) using degenerate oligonucleotide primers derived from the conserved active site of the cysteine proteinase. the 5' and 3' regions of the gene were amplified using a pcr technique for the rapid amplification of cdna ends. the cloned gene has an open reading frame of 687 b ... | 2000 | 10780554 |
| paragonimiasis westermani with multifocal lesions in lungs and skin. | we report a case of paragonimus westermani infection with a reticulonodular lesion in the right lung, left pleural effusion, and a mobile subcutaneous mass. analyses of pleural effusion and bronchoalveolar lavage fluid (balf) showed marked eosinophilia and high levels of eosinophil cationic protein and interleukin (il)-5. transbronchial lung biopsy revealed the presence of pneumonia with mild eosinophilic infiltration but remarkable lymphocytic infiltration. in this patient, high il-5 levels in ... | 2000 | 10830191 |
| paragonimus westermani: a cytosolic glutathione s-transferase of a sigma-class in adult stage. | we purified cytosolic glutathione s-transferase (gst) of adult paragonimus westermani monitoring its activity with 1-chloro-2,4-dinitrobenzene (cdnb). the enzyme was purified 18.4-fold to electrophoretic homogeneity with 21% recovery rate through a three-step procedure. the purified enzyme (pw28gst) has a subunit molecular weight of 28 kda with an isoelectric point at 4.6. monoclonal antibody (anti-pw28gst) against pw28gst did not cross-react with gsts from other helminths. cdna library was cons ... | 2000 | 10831383 |
| cysteine protease secreted by paragonimus westermani attenuates effector functions of human eosinophils stimulated with immunoglobulin g. | an immunoglobulin g (igg)-coated surface, such as that found on helminth parasites, is one of the most effective physiologic stimuli for eosinophil activation. the cysteine proteases secreted by tissue-invasive helminth larvae play an important role in evasion of the immune response by degrading the host immunoglobulins. in this study, we investigated whether cysteine proteases in the excretory-secretory product (esp) produced by paragonimus westermani newly excysted metacercariae (pwnem), which ... | 2001 | 11179333 |
| erhaia (gastropoda: rissooidea): phylogenetic relationships and the question of paragonimus coevolution in asia. | the human lung fluke paragonimus is transmitted by gastropod taxa of two superfamilies: ceritheoidea and rissooidea. the question whether or not paragonimus shows the same specificity of host-parasite coevolved relationship as the human blood fluke schistosoma was inspired by the finding of two sympatric snail species as hosts for paragonimus skrjabini in fujian province, china: gammatricula and erhaia. the former species can clearly be classified as pomatiopsidae: triculinae. the latter has pre ... | 2000 | 11220760 |
| gene sequencing for identification of paragonimus eggs from a human case. | to identify the etiologic agent from a paragonimiasis patient using molecular techniques. | 2000 | 12567661 |
| [investigation of paragonimus westermani infection in juvenile crabs in pargonimiasis endemic area in jing'an county, jiangxi province]. | | 2002 | 12567987 |
| [improvement in the preparation of paragonimus westermani chromosome for karyotype analysis]. | to improve paragonimus westermani chromosome preparation technique. | 2002 | 12567992 |
| clinical features of paragonimiasis cases recently found in japan: parasite-specific immunoglobulin m and g antibody classes. | we retrospectively analyzed clinical features in 30 patients who were referred to our laboratory and given a diagnosis of paragonimus westermani infection in 1999. our results indicate that pleurisy with eosinophilia and dominant immunoglobulin (ig) m antibody are characteristic features of the early stage of paragonimiasis, whereas igg antibody is dominant in the late stage. thus, in addition to tests for parasite-specific igg antibody, tests for igm-class antibody should always be considered f ... | 2001 | 11360226 |
| [paragonimiasis westermani caused by ingestion of chinese freshwater crabs]. | we encountered three cases of paragonimiasis westermani caused by ingestion of chinese freshwater crabs. all patients are chinese living in japan who had a history of eating raw shanghai crabs at the chinese restaurant owned by one of them. a few months later they complained of cough, chest pain and dyspnea. on examination, their peripheral blood showed a remarkable increase in eosinophils. chest radiographs revealed the presence of pleural effusion in all cases, and of pneumothorax in one. pleu ... | 2003 | 12772598 |
| molecular cloning and characterization of a mu-class glutathione s-transferase from clonorchis sinensis. | in biliary passages, clonorchis sinensis causes epithelial hyperplasia and is assumed to promote carcinogenesis. glutathione s-transferase (gst) is an antioxidant enzyme involved in phase ii defense in trematodes. a clone (pcsgstm1) encoding a gst was identified by screening a c. sinensis cdna library with a pcr-synthesized cdna probe. the predicted amino acid sequence encoded by pcsgstm1 cdna had a high degree of sequence identity and folding topology similar to the mu-class gsts. the estimated ... | 2001 | 11377741 |
| persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens. | antibody responses in serum and cerebrospinal fluid (csf) samples from patients with active and chronic paragonimiasis and in sera from patients on whom follow-up studies were done after praziquantel treatment were analyzed using antigens of paragonimus westermani prepared from eggs, metacercariae, juveniles of 4- and 7-week old, adult worms and recombinant protein of 28 kda cruzipain-like cysteine protease (rpw28ccp). the patient sera/csfs of active and chronic paragonimiasis revealed strong an ... | 2000 | 10905068 |
| partial characterization of a 17 kda protein of clonorchis sinensis. | a 17 kda protein from clonorchis sinensis adults was purified by a procedure including sephacryl s-200 hr gel filtration and q-sepharose anion exchange chromatography. the protein was proved to be a cysteine protease as it showed hydrolytic activity toward cbz-phe-arg-amc in the presence of dithiothreitol and was inhibited by specific inhibitors such as iodoacetic acid or trans epoxy-succinly-l-leucyl-amido(4-guanidino) butane. the polyclonal antibody raised against the protein reacted to 17 kda ... | 2000 | 10905071 |
| excretory-secretory product of paragonimus westermani newly excysted metacercariae inhibits superoxide production of granulocytes stimulated with igg. | it is well known that the cysteine proteases in excretory-secretory product (esp) of paragonimus westermani newly excysted metacercariae (pwnem) are capable of degrading igg in vitro. recent evidence suggests that the igg-coated surface, such as found on parasites, is one of the most effective physiologic stimuli for granulocyte activation. therefore, this study was designed to investigate the effect of excretory-secretory product (esp) of pwnem on superoxide production of granulocytes stimulate ... | 2000 | 10905073 |
| [the organ pathology in the acute stage of tissue helminthiases: the role of eosinophilia of the blood and tissues, blood immunoglobulins e and g4 and immune response-inducing factors]. | the pathogenesis of organic lesions in the early phase of tissue-dwelling helminthic infections has been analyzed and discussed by taking into account the previous findings [8, 9, 12], the results of current clinical and immunological studies [10, 11, 60] in the light of a fundamental literature data review. the products secreted by invasive helminthic larvae and the substances expressed onto their cuticula tegument surface mimic nonspecific [34, 43] and specific [21, 28, 33, 42] modulators of h ... | 2000 | 10981403 |
| separation of calcareous corpuscles from plerocercoids of spirometra mansoni and their binding proteins. | calcareous corpuscles, known as mineral concretions in platyhelminthes, may have special biological functions. in the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of spirometra mansoni (sparganum) using ficoll. purified calcareous corpuscles were reacted with crude extracts of platyhelminthes, including sparganum. proteins commonly binding to calcareous corpuscles were molecules of 10, 17, 22, and 35 kda. a 95-kda molecule was found to bind ... | 2000 | 11002990 |
| in vitro effect of praziquantel on paragonimus westermani by light and scanning electron microscopic observation. | the effect of praziquantel on p. westermani exposed in vitro was observed by stereomicroscope, light microscope and scanning electron microscope. following results were found. the worms incubated in 0.01 micro-g/ml praziquantel were moving after 26-hour incubation. however, all of them were immobilized immediately after incubation in solutions over 0.1 micro-g/ml concentration. all of the exposed worms showed severe vacuolization not only in tegument but in subtegument, intestine, ovary, testis, ... | 1987 | 12886079 |
| proteolytic activity of cysteine protease in excretory-secretory product of paragonimus westermani newly excysted metacercariae pivotally regulates il-8 production of human eosinophils. | this study investigated the effect of the excretory-secretory product (esp) of paragonimus westermani newly excysted metacercariae (pwnem) on il-8 production of human mature eosinophils. treatment of eosinophils with lower concentrations (0.3 and 1 microg/ml) of the esp significantly (p < 0.01) induced il-8 production, whereas treatment of cells with higher concentrations (3 and 10 microg/ml) did not. this effect of the esp was concentration-dependent. interestingly, the amount of il-8 productio ... | 2000 | 11012979 |
| schistosomiasis malayensis-like infection among the penan and other interior tribes (orang ulu) in upper rejang river basin sarawak malaysia. | a serosurvey of various indigenous interior tribes (orang ulu) in upper rejang river basin sarawak malaysia, the site of a multibillion ringgit hydroelectric power project, found 6.8% of the individual surveyed were seropositive for schistosomiasis, as determined by elisa method using the soluble egg antigen of schistosoma malayensis baling strain. in all age group, the seroprevalence rate is higher (9.5%) in males than in females (4.5%) except for the 31-40 age group. seroprevalence of schistos ... | 2001 | 11485090 |
| paragonimus westermani: cloning of a cathepsin f-like cysteine proteinase from the adult worm. | | 2001 | 11560415 |
| characterization and large-scale expression of the recombinant cysteine proteinase from adult clonorchis sinensis. | cysteine proteinases play important roles in the pathogenesis of several parasitic infections and have been proposed as targets for the structure-based approach of drug design. as the first step toward applying this strategy to design inhibitors as antiparasitic agents for clonorchis sinensis, we overexpressed and characterized the 24-kda cysteine proteinase from adult worms. first, the partial cysteine proteinase gene from c. sinensis was cloned by performing reverse transcription polymerase ch ... | 2001 | 11780837 |
| [a survey on intestinal parasites of soldiers in korea] | total of 2,643 korean soldiers were examined of their stool for parasitic infections by both cellophane thick smear and formalin-ether concentration techniques from august 1983 to december 1985. out of them, 73.6% were free from any parasite, 22.6% were ova positive and 4.0% cyst positive. the ova positive rates by species were ascaris lumbricoides 2.0%, trichuris trichiura 13.0%, hookworm and trichostrongylus orientalis 0.08% respectively, clonorchis sinensis 7.6%, metagonimus yokogawai 1.1%, p ... | 1986 | 12886100 |
| case report: paragonimiasis westermani with seroconversion from immunoglobulin (ig) m to igg antibody with the clinical course. | a 66-year-old man visited our hospital with primary complaint of cough. chest roentgenogram showed slight pleural effusion and pneumothorax in the left lung. eosinophilia (22.8%) was also found in his peripheral blood. multiple-dot enzyme-linked immunosorbent assay (dot-elisa) for the detection of parasite-specific immunoglobulin (ig) g antibody was used to screen his serum against various parasitic diseases, but no significant binding was observed with any of the 12 parasite antigens examined, ... | 2001 | 11791983 |
| recombinant paragonimus westermani yolk ferritin is a useful serodiagnostic antigen. | a recombinant protein of paragonimus westermani yolk ferritin was bacterially produced from a previously cloned complementary dna and was used as an antigen for an enzyme-linked immunosorbent assay (elisa) against paragonimiasis- and other helminth-infected sera to evaluate its serodiagnostic potential. the elisa revealed that paragonimiasis westermani had 88.2% sensitivity and 100% specificity. the positive and negative predictive values of the elisa were calculated to be 100% and 97.1%, respec ... | 2002 | 12001061 |
| structural and immunological characteristics of a 28-kilodalton cruzipain-like cysteine protease of paragonimus westermani expressed in the definitive host stage. | a complete cdna sequence encoding a 28-kda cruzipain-like cysteine protease of adult paragonimus westermani, termed pw28ccp, was isolated from an adult cdna library. the cdna contained a single open reading frame of 975 bp encoding 325 amino acids, which exhibited the structural motif and domain organization characteristic of cysteine proteases of non-cathepsin bs including a hydrophobic signal sequence, an erfnin motif, and essential cysteine residues as well as active sites in the mature catal ... | 2000 | 11063501 |
| purification and characterization of a 7-kda protein from clonorchis sinensis adult worms. | a 7-kda protein was purified from extracts of adult clonorchis sinensis by a combination of ammonium sulfate precipitation, anion exchange chromatography, cation exchange chromatography, gel-filtration chromatography, and reversed-phase fplc. the 7-kda protein exists in the excretory-secretory products of adult c. sinensis, but not in extracts of adult paragonimus westermani. also, the 7-kda protein reacted with the sera of patients with clonorchiasis but not with paragonimiasis or normal human ... | 2002 | 12099418 |
| active hepatic capsulitis caused by paragonimus westermani infection. | paragonimiasis is an important re-emerging parasitosis in japan. although the lungs and pleural cavity are the principal sites affected with the parasite, ectopic infection can occur in unexpected sites such as skin and brain. this case report describes a patient with active hepatic capsulitis due to paragonimus westermani infection. the patient was successfully treated with praziquantel at the dose of 75 mg/kg/day for 3 days. | 2002 | 12211538 |
| elevated levels of thymus and activation-regulated chemokine (tarc) in pleural effusion samples from patients infested with paragonimus westermani. | to investigate the pathogenic mechanisms of eosinophilic pleural effusion in patients with paragonimiasis, we measured the levels of various chemokines including thymus and activation-regulated chemokine (tarc), eotaxin, rantes and il-8 in pleural effusion samples. samples were obtained from 11 patients with paragonimus westermani infection, six patients with pleural transudate, eight with tuberculous pleurisy and five with empyema. high percentages of eosinophils were detected in pleural fluid ... | 2002 | 12390321 |
| [development of paragonimus westermani ichunensis in a reservoir host]. | experimental observations showed, that the muscle larva stage of the trematodes paragonimus westermani ichunensis located in a reservoir host can enlarge its body size. a rate of growth in trematode individuals varied both among different host animals and within one host specimens. when rodents are infected with such muscle larvae, the individuals with the size exceeding 1.15 x 0.33 mm invade into the lungs oh host, while smaller individuals secondarily migrate to muscles. | 2002 | 12481611 |
| heterotopic paragonimiasis in the omentum. | paragonimus westermani is a lung fluke but may be found in organs other than the lungs. a case of omental paragonimiasis was found incidentally by plain radiography and computed tomography and showed multiple, irregularly shaped, conglomerated calcifications in the intraperitoneum. many p. westermani ova were detected in the resected omentum. this uncommon calcification on the plain abdominal radiography and computed tomography scan was considered to represent radiologic findings of ectopic para ... | 2002 | 12488753 |
| hybridoma cell agglutination as a novel test to detect circulating antigen of schistosoma japonicum. | we developed a serodiagnostic test which is based on the agglutination of hybridoma cells. in the presence of specific antigen, agglutination of the fixed and stained cells occurs and can be visualized in analogy to traditional erythrocyte agglutination. the procedures were developed with a murine cell line producing a monoclonal antibody against a schistosome gut protein and sera of patients and mice infected with schistosoma japonicum. this test is capable of detecting circulating antigen duri ... | 2003 | 12535254 |
| [effect of triclabendazole on the ultrastructure of body wall and vitelline cells of paragonimus westermani]. | to observe the ultrastructural changes in the body wall and the vitelline cells of paragonimus westermani in vitro and in vivo before and after triclabendazole treatment. | 1999 | 12563729 |
| an epidemiologic study on clonorchiasis and metagonimiasis in riverside areas in korea. | a study was carried out to figure the cases and to observe the endemic status of clonorchiasis and metagonimiasis in endemic areas in korea. total 13,373 inhabitants living in 40 villages along 7 main rivers and 9 small streams were subjected to stool examination. they were selected randomly among the riverside population. their specimens were examined both by cellphane thick smear method and stoll's egg countung technique. this study was performed during the period from may 1979 to april 1980. ... | 1981 | 12902708 |
| [biological characteristics of the juvenile paragonimus westermani]. | to observe the biological characteristics of the juvenile of paragonimus westermani. | 1999 | 12563738 |
| [an epidemiological study on zoonoses in korea] | helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. for past several decades in korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. however, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; an ... | 1981 | 12902720 |
| phylogenetic relationship of ribosomal its2 and mitochondrial coi among diploid and triploid paragonimus westermani isolates. | we compared patterns of intraspecific polymorphism of two markers with contrasting modes of evolution, nuclear ribosomal dna (rdna) and mitochondrial dna (mtdna), in the lung fluke, diploid and triploid paragonimus westermani from three geographical regions of korea. the genetic distances between three populations of korean diploid and triploid p. westermani showed no significant difference in the nucleotide sequences of the mitochondrial cytochrome c oxidase subunit i (mtcoi) and ribosomaal sec ... | 2003 | 12666730 |
| immunoblot observation of antigenic protein fractions in paragonimus westermani reacting with human patients sera. | in order to observe the antigenic fractions in saline extract of adult paragonimus westermani, proteins in the crude extract were separated by sodium dodecyl sulfate-polyacylamide gel electrophoresis (sds-page) in reducing conditions. the separated protein fractions were transferred to nitrocellulose paper on which 20 sera from human paragonimiasis were reacted and immunoblotted. out of 15 stained protein bands in sds-page, 7 reacted with the sera. of 14 reacted bands, 30 kilodalton(kda) band wa ... | 1988 | 12811037 |
| [studies on the immunodiagnosis of rabbit clonorchiasis ii. immunoaffinity purification of whole worm antigen and characterization of egg, metacercaria and adult antigens of clonorchis sinensis] | the sensitivity and specificity of crude and affinity-purified antigens of clonorchis sinensis obtained from the infected rabbits were studied. stage-specific antigenic proteins from the eggs, metacercariae and adult worms were characterized by sds-polyacrylamide gel electrophoresis (sds-page) and enzyme-linked immunosorbent assay (elisa) the results were as follows: the antibody-binding antigen (aba) purified from whole worm crude antigen (wwa) by cnbr-activated sepharose 4b affinity chromatogr ... | 1988 | 12811052 |
| characterization and classification of five cysteine proteinases expressed by paragonimus westermani adult worm. | three new members of the cysteine proteinase gene family of paragonimus westermani have been isolated and classified. comparisons of the predicted amino acid sequences of pwcp2 (u69121), pwcp4 (u56958), and pwcp5 (u33215) were performed with those of the previously reported pwcp1 (u69120) and pwcp3 (u56865) sequence. the amino acid alignment showed conservation of the cysteine, histidine, and asparagine residue that form the catalytic triad. with 57 cysteine proteinases including pwcp1-5, we con ... | 2002 | 12856309 |
| [immunoelectrophoresis for fasciola hepatica] | in an attempt to investigate the specific antigenic substance of fasciola hepatica, ouchterlony tests and immunoelectrophoretic analyses were carried out. crude fasciola antigen was prepared and fractionated by sephadex g-200 column to antigen i, ii and iii according to protein content. crude antigens of paragonimus westermani, clonorchis sinensis and paramphistomum sp. were also prepared for control and absorption study. antiserum was prepared by injecting 0.5 ml of crude fasciola antigen with ... | 1979 | 12902763 |
| paragonimus westermani: molecular cloning, expression, and characterization of a recombinant yolk ferritin. | ferritin is an intracellular protein involved in iron metabolism. a cdna pwyf-1 cloned from the adult paragonimus westermani cdna library encoded a putative polypeptide of 216 amino acids homologous with ferritins of vertebrates and invertebrates. febinding motifs identified in pwyf-1 polypeptide were conserved and predicted to form a ferroxidase center. pwyf-1 polypeptide contained an extended peptide of 45 amino acids at its c-terminus. recombinant pwyf-1 protein, expressed and purified from e ... | 2002 | 12856317 |
| endemic foci of paragonimus westermani in chilgok, kyungpook province, korea. | by performing the county-wide surveys of paragonimus westermani infection in several areas in the chilgok county, kyungpook province, three endemic foci of paragonimus westermani were found. the foci found in this survey were haksan first dong, haksan second dong, and settom in kumhwa village, all in kasan myun, chilgok county. in the three communities, the positive rates for the fluke among the residents by the intradermal test with paragonimus antigen varied from 22.3 per cent to 25.0 per cent ... | 1977 | 12913428 |
| [ultrastructural studies on the surface of paragonimus westermani metacercaria] | the present study was undertaken to demonstrate the surface structure of paragonimus westermani metacercaria in korea with special reference to the distribution of sensory papillae. metacercariae were isolated from crayfish, one of the second intermediate host of p. westermani in bogil island, chollanam-do (province), korea, where has been known as an endemic area of human paragonimiasis. isolated metacercariae were excysted and examined with light, scanning and transmission electron microscopes ... | 1987 | 12886063 |
| detection of circulating antigens in rats experimentally infected with paragonimus westermani by elisa. | circulating antigens in rats experimentally infected with paragonimus westermani were examined by elisa. from a total of 22 albino rats, each fed with 25 metacercariae, blood samples were collected until 12 weeks after infection. the specific antibodies against p. westermani in the serum of an infected cat were purified by ammonium sulfate precipitation, deae anion-exchange chromatography and affinity chromatography serially. so-called double antibody sandwich elisa method was used for the detec ... | 1987 | 12886064 |
| purification of cystic fluid antigen of taenia solium metacestodes by affinity chromatography using monoclonal antibody and its antigenic characterization. | this study was undertaken to purify cystic fluid (cf) antigen of taenia solium metacestodes by affinity chromatography using specific monoclonal antibody (mcab) and to characterize the antigenicity of the purified antigen. the hybridoma cell lines, prepared by fusion between mouse plasmacytoma and spleen cells from balb/c mice immunized with cf, secrected antibodies reacting to various helminthic antigens. majority of cell lines reacted to cf only but some also reacted to parenchymal antigen of ... | 1986 | 12886091 |
| [purification of antigenic proteins of paragonimus westermani and their applicability to experimental cat paragonimiasis] | this study was designed to evaluate the partially purified antigens which were fractionated from crude extract of paragonimus westermani and to monitor the enzyme-linked immunosorbent assay (elisa) in experimental cat paragonimiasis during the course of infection as well as before and after chemotherapy. crude extract of 6-month-old adult p. westermani was fractionated to 5 antigens by successive applications of ammonium sulfate precipitation, ion exchange chromatography and gel filtration. and ... | 1986 | 12886094 |
| [elisa of paragonimiasis in cat by crude and purified antigens of paragonimus westermani] | enzyme-linked immunosorbent assay (elisa) using crude and affinity-purified antigens of adult worms of paragonimus westermani was performed for infected cat sera with different worm burden, from preinfection to 18th week after infection. crude antigen was used with supernatant of homogenated worms by freezing-thawing method, and the supernate was centrifuged for l hour at 10,000 rpm at 4c. affinity-purified antigen (antibody-bound antigen) was prepared from fractions (bound and unbound) of crude ... | 1986 | 12886095 |
| [preparation and characterization of monoclonal antibodies against clonorchis sinensis]. | to develop and identify monoclonal antibody (mcab) against adult clonorchis sinensis. | 1998 | 12078207 |
| [ultrastructure of the integument of adult paragonimus westermani] | the present study was performed to observe the ultrastructure of the integument of adult paragonimus westermani. dogs experimentally infected with 60 metacercariae of p. westermani were autopsied 4 months after the infection. adult p. westermani were extracted from the dogs and the fine structure was studied by means of scanning and transmission electron microscope. the findings are as follows: scanning electron microscopic findings showed that the spines and the papillae are distributed at whol ... | 1985 | 12888693 |
| [distribution of medically important freshwater snails and larval trematodes from parafossarulus manchouricus and semisulcospira libertina around the jinyang lake in kyongsang-nam-do, korea] | the jinyang lake is dammed up nam river, and surrounded by jinju city and four counties; jinyang, sanchong, hadong, and sachon in kyongsang-nam-do, korea. the area around this man-made lake have been known as an endemic focus of clonorchiasis in korea. the present study was first aimed to know the distribution of freshwater mollusks including medically important snails, and larval trematodes shed from parafossarulus manchouricus and semisulcospira libertina. in addtion to above studies, water an ... | 1983 | 12902649 |
| [on the applicability of partially purified antigenic preparations of paragonimus westermani] | in order to obtain more specific antigenic preparation for the diagnosis of human paragonimasis, crude saline extract of whole worm (=pwwwe), secretory-excretory components (pwsec) and secretion-excretion-free somatic extract (pwsm) of 12 week-old paragonimus westermani were filtrated through sephadex g-200 gel column. the adult paragonimus worms were obtained from experimentally infected dogs. a total of 11 antigenic solutions was lyophilized or diluted to adjust protein content of 1 mg/ml. to ... | 1983 | 12902657 |
| larval trematodes from semisulcospira snails in kyungpook province, korea. | in an attempt to determine the incidence of the cercarial larvae of trematodes, semisulcospira snails collected in judong stream, an endemic area of paragonimus westermani, in kyungpook province, korea, were examined from 1965 to 1980. the infestation rate for cercariae in the snails was 5.4 per cent, but in 1970 dropped to 4.0 per cent and remained at the same level, 4.2 per cent, in 1975. it then decreased to 3.5 per cent in 1980. seven species of cercariae, cercaria incerta(kobayashi, 1992) f ... | 1982 | 12902687 |
| [changes of liver and diaphragm in experimental paragonimiasis] | changes of abdominal organs in early paragonimiasis are described in dogs and cats. experimental infection was induced by feeding 40~50 and 20~30 metacercariae of paragonimus westermani to 15 dogs and 15 cats respectively. these animals were killed sequentially starting from 8 days to 120 days after infection. complete autopsies were carried out in all animals and the tissues were examined microscopically with the main emphasis on changes of the liver and the diaphragm. a definite bout of acute ... | 1982 | 12902688 |
| [agar-gel precipitin reactions in experimental paragonimiasis] | in an attempt to investigate the sensitivity of immunodiagnosis in cats experimentally infected with paragonimus westermani, agar-gel precipitin reaction were studied. metacercariae of p. westermani were administered to cats in various doses(2~100 metacercariae per cat) and antisera were obtained at an interval of a week. 1. precipitin bands appeared in homologous antigen-antibody in experimental paragonimiasis between 3 and 5 weeks after infection in all the cats. 2. almost all the cases in whi ... | 1981 | 12902705 |
| a human case of house dust mite tarsonemus floricolus collected from sputum. | a 23-year-old medical student showed a positive reaction on a skin test for paragonimus westermani, and two tarsonemus floricolus mites were subsequently found by sputum examination and identified morphologically. our report is the first human case of tarsonemus floricolus in korea. | 2003 | 12972731 |
| studies on the biological aspects of the larval stages of paragonimus westermani, especially the invasion of the second intermediate hosts. | | 1952 | 13010924 |
| studies on paragonimiasis in shizuoka prefecture i. an epidemiologic survey of paragonimus westermani along the banks of the kanko river. | | 1952 | 13034368 |
| paragonimus westermani infestation involving the anterior abdominal wall; report of a case simulating acute appendicitis. | | 1955 | 13271142 |
| [paragonimus westermani (ringeri) infection in man (haemoptysis parasitaria endemica). i. epidemiology and clinical picture]. | | 1960 | 13707905 |
| [paragonimus westermani (ringeri) infection in man (haemoptysis parasitaria endemica). i. epidemiology and clinical picture]. | | 1960 | 13707906 |
| [paragonimus westermani (ringeri) infection in man (hemoptysis parasitaria endemica). ii. therapy and prophylaxis]. | | 1960 | 13707907 |
| clinical evaluation of praziquantel(embay 8440; biltricide(r)) in the treatment of paragonimus westermani. | a total of 52 paragonimiasis patients was treated with praziquantel at three dose levels: 21 patients received 3 x 25.0 mg/kg bwt on a single day, 21 patients were treated with 3 x 25.0 mg/kg for 2 consecutive days and 10 patients were treated with the same dose for 3 consecutive days. follow-up examination were carried out at monthly up to 4 months (120 days) after treatment. fifteen (71.4%) out of 21 patients who received the drug 3 x 25.0 mg/kg bwt on a single day were parasitologically cured ... | 1981 | 12902716 |
| infection experiment of paragonimus westermani in its first intermediate host, semisulcospira libertina. | | 1961 | 13752878 |
| clonorchiasis and paragonimiasis in the republic of korea. report on a prevalence survey using intradermal tests. | until recently, information on the distribution of and prevalence of infection with the liver fluke, clonorchis sinensis, and the lung fluke, paragonimus westermani, was based almost entirely on surveys by stool or sputum examination, involving techniques insufficiently sensitive and too costly and time-consuming to allow of really adequate coverage. however, a simple and reliable intradermal test for these infections has now been developed, based on the use of purified antigens, and this has be ... | 1959 | 13842753 |
| paragonimus westermani (szechuan variety) and a new species of lung fluke--paragonimus szechuanensis. ii. studies on clinical aspects of paragonimasis szechuanensis--a new clinical entity. | | 1962 | 13879399 |
| the route of migration of the larva of paragonimus westermani in the final hosts. | | 1962 | 14009141 |
| splenic paragonimiasis: a case in a dog. | a case of paragonimus westermani cysts found deep inside the spleen is described. this spleen was from a dog that was experimentally infected via oral route with a hundred metacercariae and was killed 3 months after. at autopsy, beside the usual lesions due to paragonimus in the lungs, the spleen was the site of multiple parasitic cysts. the involved spleen was slightly enlarged and showed an area of hemorrhagic dome on the capsular surface. there found a total of 8 adult worms in four well dema ... | 1981 | 12902722 |
| epidemiological study of paragonimus westermani in wiseong county, kyungpook province, korea. | this survey was undertaken to determine the recent prevalence of lung fluke, paragonimus westermani, in the three localities of wiseong county, kyungpook province, korea and to compare the prevalence among the residents in the same areas between 1924 and 1980. the three localities, ku-seong, sam-chun, and um-ji villages (dong) have been known as the endemic areas of paragonimus westermani. the surveys were made on the general population. they were examined using intradermal test with paragonimus ... | 1980 | 12902733 |
| [lung findings in experimental paragonimiasis] | a pathological study was done to elucidate sequential changes of the lungs in various time intervals following experimental paragonimiasis in 15 dogs and 15 cats. the dogs and cats were fed with 30~50 metacercariae of paragonimus westermani, and were sacrificed at 15, 20, 30, 45, 60, 90 and 120 days after infection respecively. autopsies were performed immediately after death. gross and microscopic examination of the lungs showed following findings: 1. there were no qualitative difference in pat ... | 1979 | 12902754 |
| [a histopathologic study of the lungs infected with paragonimus westermani in the dog] | pulmonary paragonimiasis is one of the most important endemic parasitic disease in korea. although it is well known that paragonimus westermani invades into the lung tissue eventually resulting respiratory failure, the pathogenesis of inducing lung lesions is not fully elucidated. it is still debatable on the nature of morphological component of 'cyst' which is known to be the most consistent change in lung paragonimiasis. for the purpose of clarification of morphological changes associated with ... | 1979 | 12902758 |
| paragonimus westermani (kerbert, 1878) in thai leopards. | | 1964 | 14212886 |
| parasitism of paragonimus westermani metacercareae in potamon crabs. | | 1965 | 14281352 |
| an ecological study of paragonimus in malaysia. | an ecologic study on paragonimus in malaysia was attempted from may to september 1967. seven streams located in various directions and distances from kuala lumpur were surveyed for the study of intermediate hosts, snail and crab. one malayan village and one aborigine village where infected crabs were found, and two tuberculosis hospitals in k.l. were surveyed for the study of human population. intradermal tests along with sputum or stool examination to detect human infection by paragonimus were ... | 1978 | 12902783 |
| [precipitin reactions between host organ extracts and antisera for paragonimus and clonorchis] | precipitin reaction between host organ extracts and antisera for paragonimus and clonorchis were studied to know whether the antigens from two species of trematodes have common characters with those of host organs. the brain, lung, heart, liver and muscle of rat and rabbit were extracted in 0.1% saline solution by freezing-thawing technique. for immunization of rabbit, the extracts of paragonimus and clonorchis was emulsified with equal amount of freund's adjuvant and 1.0 ml of the mixture was i ... | 1976 | 12913436 |
| the origin of the triploid in paragonimus westermani on the basis of variable regions in the mitochondrial dna. | triploid, parthenogenetic forms of the lungfluke, paragonimus westermani, occur in japan, korea and china. the origin(s) of triploidy has been debated over the years. sequences of two regions in the mitochondrial dna, i.e. partial lrrna (16s), and a portion of the non-coding region, were obtained from natural populations of p. westermani. all triploid individuals (japan, korea, china) and a single tetraploid individual (china) had identical sequences in the 16s region studied. some sequence vari ... | 2003 | 14627442 |
| [cloning and identification of the gene fragments of paragonimus westermani]. | to screen and identify the recombinants from the cdna library of the adult paragonimus westermani(pwa) for immunodiagnosis and immunoprophylaxis. | 2003 | 14628343 |
| expermental chemotherapeutic effects of niclofolan (bayer 9015, bilevon) on the animals infected with paragonimus westermani or p. iloktsuenensis. | the new drug niclofolan (bayer 9015, bilevon), 5,5'-dichloro-2,2'-dihydroxy-3, 3'-dinitrobiphenyl, has been used in the treatment of paragonimus westermani in dogs and cats and p. iloktsuenensis in rats. it was proved that administration of daily dose of 1.0 mg/kg body weight for 3 days or in 2 doses of 2.0 mg/kg body weight by alternate days were evidently effective for the infected dogs, cats and rats with the lung flukes, and toxic manifestations were not found. | 1976 | 12913443 |
| [histochemical study on trematodes: distribution of carbonic anhydrase activity] | the purpose of the present study is to demonstrate the distribution of carbonic anhydrase pattern in the various termatodes (fasciola gigantica, paramphistoma orthocoelium, paragonimus westermani) by means of kurada staining method, and to correlate these findings with the histochemical data and harboring location. the results are summarized as follows: 1. in fasciloa gigantica, carbonic anhydrase activity was positive in the vitelline gland cells and eggs in the uterus. 2. in paramphistoma orth ... | 1972 | 12913508 |
| [immuno-diffusion and complement-fixation test in paragonimiasis of cat] | in order to observe the complement fixation test and immuno-diffusion test of paragonimiasis, the sera taken at 10 days intervals up to 150 days from cats infected with paragonimus westermani were examined by the above two immunological methods. the resultant findings were as follows: 1)the complement fixation test showed positive reaction 20 days after the infection with 20 metacercariae, and 40~50 days after the infection with 10 metacercariae. the highest titer was observed 110 days later fol ... | 1969 | 12913529 |
| infestation status of paragonimus westermani metacercariae in the second intermediate host in ulchin county, kyungpook province. | a study on the population density of crayfish intermediate hosts and infestation status of crayfish with encysted larvae of paragonimus westermani in ulchin county, kyungpook province, korea was conducted from may to october in 1986. the population density of the crayfish ranged from 1 to 13, with an average of 4 per manhour. among the six habitats, ducheon had a somewhat higher density than that of the others. of eight hundred and seventeen crayfish examined, 127 or 15.5 per cent harboured the ... | 1986 | 12886096 |
| [detection of schistosomia japonicum 5d gene by polymerase chain reaction and genechip technique]. | in order to develop the diagnostic genechip for specific detection of schistosoma japonicum (chinese mainland strain). | 2004 | 15132872 |
| evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. | the applicability of micro-elisa was evaluatd in human neuro-cysticercosis using paired samples of serum and csf. a total of 355 cases who were mostly neurologic patients was subjected. cystic fluid of c. cellulosae was used as antigen in protein concentration of 2.5 micro-g/ml. serum was diluted to 1:100 and csf was undiluted in the assay for the specific igg antibody level. the differential criterion of the positive reaction was the abs. of 0.18 in both samples. the results were summarized as ... | 1986 | 12886105 |
| cloning and characterization of a new cysteine proteinase secreted by paragonimus westermani adult worms. | the cysteine proteinases of paragonimus westermani are known to play important roles in invasion and pathogenesis to hosts and in immune modulation and nutrient uptake. in this study, we have cloned a new cysteine proteinase of p. westermani, pwcp2, from adult worms and tested its diagnostic usefulness. the pwcp2 gene had an open reading frame of 816 base pairs and a conserved catalytic triad of cysteine, histidine, and asparagine residues. the mature form of recombinant pwcp2 (rpwcp2) lacking a ... | 2004 | 15238695 |
| [antigenicity of the soluble egg antigen of paragonimus westermani] | to evaluate the immature eggs of paragonimus westermani as a source of diagnostic antigen, about a million eggs which were excreted by 104 adult worms were collected; their saline extract (soluble egg antigen; pwsea) was prepared. the specific igg and igm antibody levels were observed in experimental dog paragonimiasis by micro-elisa, using pwsea as well as whole worm extract of 12 week-old p. westermani (pwwwe). the protein composition of the pwsea was observed by disc-page. the results could b ... | 1986 | 12886107 |
| electrophoretic patterns of proteins from paragonimus westermani in early developmental stages. | in order to observe the protein compositions of soluble extracts of p. westermani, and their changes during early developmental stages, the crude saline extracts of 4, 6, 8, 10 and 12 week-old worms which were harvested from experimentally infected dogs were analysed by disc-page. the results were as follows: a total of 15 bands were identified from electrophoregrams of respective developmental stages. of them, 5 bands were recognized throughout the developmental stages. the number and protein a ... | 1985 | 12888660 |